Year : 2005

Total records found: 9

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_0015	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.4	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UCACUGUUAUCCGAUAGCAGCGCGGGAUGA-GGGUCAAUGCGUCAUA	88	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	2.0 μg of RNA aptamer S-PS8.4 effected ca. 71% inhibition of cell invasion by pil+ S. enterica serovar Typhi A21-6 but only ca. 19% inhibition in the case of the pilS::Kmr mutant.	8	Nitrocellulose Filter Binding Assay	8.56 nM	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	Best Candidate	N/A	N/A
ABdb_0016	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.3	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AUUACCUAGAGCGGGAUAAAGUAUAGGUU-GGGUCAAUGCGUCAUA	87	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	N/A	8	Nitrocellulose Filter Binding Assay	N/A	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0017	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.2	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-CAUCGAGGAGGCGGGAUAUUCGAUGAGUU-GGGUCAAUGCGUCAUA	87	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	N/A	8	Nitrocellulose Filter Binding Assay	N/A	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0018	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.5	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-GAGUACGAGCGGGAUAGUAAUCGGGUGAU-GGGUCAAUGCGUCAUA	87	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	N/A	8	Nitrocellulose Filter Binding Assay	N/A	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0019	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.6	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-GAAGGGGUCCGGCUCGGGUGAGGGUCGGGU-GGGUCAAUGCGUCAUA	88	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	N/A	8	Nitrocellulose Filter Binding Assay	N/A	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0020	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.9	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AGCUAGCGGGGGGGCUCGACGGUGGUGGGUU-GGGUCAAUGCGUCAUA	89	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	N/A	8	Nitrocellulose Filter Binding Assay	N/A	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0021	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.7	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UAGCGGGAGCUUGGACCUGGUGGUCGCGGC-GGGUCAAUGCGUCAUA	88	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	N/A	8	Nitrocellulose Filter Binding Assay	N/A	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0022	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.1	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UUGGGAUCAGCUCGGCGCUGGAGGAGGGGC-GGGUCAAUGCGUCAUA	88	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	N/A	8	Nitrocellulose Filter Binding Assay	N/A	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0023	16189080	2005	Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi	S-PS8.8	GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AACUGUACAUGGGCGCAACAGGGAGUUAGC-GGGUCAAUGCGUCAUA	88	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssRNA	Salmonella Typhi	Type IVB Pili structural proteins	Identify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.	N/A	8	Nitrocellulose Filter Binding Assay	N/A	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A