Target Organism : Vibrio Parahaemolyticus

Total records found: 88

Download:

Search:
AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0125 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA3PATAGGAGTCACGACGACCAGAA-TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.Showed a high binding affinity of 76% for V. parahemolyticus and a low apparent binding affinity of 4% for other bacteria.9Flow Cytometry16.88 ± 1.92DetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0126 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA1TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG405'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A1 was 64%.9Flow Cytometry22.92 ± 4.72 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0127 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA1PATAGGAGTCACGACGACCAGAA-TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A1P was 67%.9Flow Cytometry21.45 ± 2.62 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0128 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA3TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT405'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A3 was 75%.9Flow Cytometry24.03 ± 5.18 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0129 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA17PATAGGAGTCACGACGACCAGAA-AGGCCGGCCCCTCTGAACTAGATGCAGGGGAGGCGAGCGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0130 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA18PATAGGAGTCACGACGACCAGAA-GCGGGCAGCTCCACCGGTAGGCTCCGAGTCATCACGGTCG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A18P was 62%.9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0131 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA21PATAGGAGTCACGACGACCAGAA-TTTTGACGAAGTCAGTGCGTCGAAGCGCAAGCATTACAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0336 242670762013A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labelsApt1ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellAptamer-modified QDs (QD-apt) were developed to selectively capture and simultaneously detect the target bacteria.For V. parahaemolyticus cells, a linear range of 3.4 × 10(4) to 3.4 × 10(7) cfu/mL was obtained with QD 535-apt 1-FCM, and a detection limit of 5 × 10³ cfu/mL was achieved.N/AN/AN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0431 245686252014Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labelsApt₂ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a multiplex method for the simultaneous detection of three pathogenic bacteria using multicolour upconversion nanoparticles (UCNPs) coupled with aptamers.The linear range showed from 50-10(6) cfu/mL, and the limits of detection were 10 cfu/mL for V. parahemolyticus.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0434 246505832014A universal fluorescent aptasensor based on AccuBlue dye for the detection of pathogenic bacteriaVP-aptTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellAptamer is hybridized with complementary DNA (cDNA) to form fluorescent dsDNA.Exhibit a linear concentration range of 50 to 10(6) cfu/mL, and the LOD was 35 cfu/mL in 1.5 h for V. parahaemolyticus.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0461 https://doi.org/10.1007/s00604-014-1406-32014Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles.Apt1 ( V. parahaemolyticus aptamer)ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDevelop a dual fluorescence resonance energy transfer (FRET) from gQDs and rQDs and amorphous carbon nanoparticles (CNPs) for simultaneous detection of V. parahaemolyticus and S. typhimurium.LOD: 25 cfu/mL and linear range from 50 to 10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0692 263022562015Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticusApt 1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a colorimetric aptasensor system based on sandwich-type complex (AuNPs−HRP−aptamer−target−aptamer−MNPs) to detect V. parahemolyticus.Linear detection range: 10 to 10^6 cfu/mL, with a limit of detection (LOD) of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0693 263022562015Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticusApt 2TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a colorimetric aptasensor system based on sandwich-type complex (AuNPs−HRP−aptamer−target−aptamer−MNPs) to detect V. parahemolyticus.Linear detection range: 10 to 10^6 cfu/mL, with a limit of detection (LOD) of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0821 https://doi.org/10.1016/j.foodcont.2015.11.0312016Vibrio parahaemolyticus detection aptasensor using surface-enhanced Raman scatteringAptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellA SERS-based aptasensor was developed using Au@Ag core-shell nanoparticles for the detection of V. parahaemolyticus.Achieved a concentration in the linear range of 10–10(6) cfu/mL, with a limit of detection of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (for apt 1) and 5'-Cyanine3 (Cy3) Labeled (for apt 2)N/AN/AN/AN/AN/A
ABdb_0999 https://doi.org/10.1007/s00604-017-2383-02017Rolling circle amplification based amperometric aptamer/immuno hybrid biosensor for ultrasensitive detection of Vibrio parahaemolyticusVp-aptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped an antibody-aptamer-based hetero-sandwich amperometric biosensor for the detection of V. parahaemolyticus.Achieved a range of 2.2 to 2.2 x10(8) cfu/mL, and the limit of detection is as low as 2 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AAfter 2 weeks of storage at 4°C, the fabricated electrochemical aptasensor retains about 95.2% of its initial sensing current response.N/AN/AN/A
ABdb_1048 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-2TCAGCACGT-ATAAGCATGAATTGACCAACCTAAACTTATTCATTTTCCAGCACCTCTAATATTACTGGC-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.75% binding affinity to V. parahaemolyticus than to other foodborne bacteria (less than 18%).8Flow Cytometry10.3 ± 4.5 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1049 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-1TCAGCACGT-AGCCCTATTTGTTTTCTCTTGTCTCTGTACTGCTGATGTTGGTCATTCTCTTTTCTCGGT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry19.2 ±  2.8  nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1050 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-3TCAGCACGT-TATCTAGTCAGATATCCAGACAGTCGCGGCTGGAAGCTTCTGTTAAGAATTTGAGATACT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry25.2  ±  3.1 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1051 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-4TCAGCACGT-GCGGGCATTATTGCACTCCACGGCGAGTAGTGCTCACAGAGTCATTTACACCGGTCGTAT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry17.2  ±  5.1 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1151 297773092018Fluorometric determination of Vibrio parahaemolyticus using an F0F1-ATPase-based aptamer and labeled chromatophoresV. parahaemolyticus aptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped an F0F1-ATPase-based aptasensor for the fluorometric determination of V. parahaemolyticus.The relative fluorescence varies linearly over the 15 to 1.5 × 10(6) cfu/mL Vibrio parahaemolyticus concentration range, and the limit of detection is 15 cfu/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1152 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#1ATACCAGCTTATTCAATT-CCATGGTCCCTCGTGTTTATTATGTTGTCTGAACTGGCTG-AGATTGCACTTACTATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.VPCAapta#1 was significantly higher (798.41 ng/ul) than that of the other aptamer.11Surface Plasmon Resonance (SPR)2.04 ± 0.12 nMDiagnosticWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1153 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#2ATACCAGCTTATTCAATT-CCACGTTTCCACGTATCCTCGGAGCTTGCTTAACCGTACG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1154 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#3ATACCAGCTTATTCAATT-TGCGTAGTTCTTTTAACAACCATTCAGCGTATTTTCGTGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1155 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#4ATACCAGCTTATTCAATT-CCCTCGGCGTAACTAGTACCGTTGCACCACCAACGTGATT-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1156 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#5ATACCAGCTTATTCAATT-CGCAAGGGATTTCGGACCGGGCTTGGTCACACACAGAGCA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1157 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#6ATACCAGCTTATTCAATT-CCAGCAGTGGGGCATGGGGGAACGATAAGATTATAAGGGGG-AGATAGTAAGTGCAATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1158 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#7ATACCAGCTTATTCAATT-CACGCAAGCAGAAGCCACTCCCCCTGGTCGTACTATTTAG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1159 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#8ATACCAGCTTATTCAATT-CACAGGCGTACCACTGCCCCAACGATCCTTTTGGTTACCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1160 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#9ATACCAGCTTATTCAATT-GTCGGAGAGCCTGGCCGTCTGCTCGTAAGTACTATCATAGC-AGATAGTAAGTGCAATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1161 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#10ATACCAGCTTATTCAATT-TGGAGAAGCGGCAGTTGATGCCTGGTACCCGTCTGCTACG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1162 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#11ATACCAGCTTATTCAATT-CGTAAAGGACGCTGCCATGAATGTGCTATCCAAGCCTGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1163 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#12ATACCAGCTTATTCAATT-CGTAAAGAACGCTGCCATGAATGTGCTATCCAAGCCTGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1164 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#13ATACCAGCTTATTCAATT-CGTAAGAACGCTGCCATGAATGTGCTATCCAGGCCTGGG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1165 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#14ATACCAGCTTATTCAATT-CAACGGAGGGAAGTTTCCATGTCCGGTACCAAGCGGTTTTTG-AGATAGTAAGTGCAATCT785'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1166 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#15ATACCAGCTTATTCAATT-GCACGGGAGGCACCCTGACATTGCAAATCCCATCGGTGTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1167 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#16ATACCAGCTTATTCAATT-ACCTATAACAGCCATCATACGAGGTAATCCCCTCCCTCGA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1168 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#17ATACCAGCTTATTCAATT-CCAACTGGATCTTACTGAGGAACGGTCCATTAGCACATCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1169 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#18ATACCAGCTTATTCAATT-CGCGACCACATCCTGCGTAAACACATCTCCGCCAGTCCAT-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1170 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#19ATACCAGCTTATTCAATT-CGCAAGAGCAGCATACAGCACGAACGAGCCATTTACGCCA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1171 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#20ATACCAGCTTATTCAATT-CGAAGACCAGACATGGTAGCCACGCATAGTCCAACTTAAG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1172 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#21ATACCAGCTTATTCAATT-ACACCACACTAATGCATGCATTCTGTGAGTCACAGTTCA-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1173 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#22ATACCAGCTTATTCAATT-CACTCAAACCCAAACCATGCAAACTGAACAACGCAACACA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1174 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#23ATACCAGCTTATTCAATT-CACCAAACTGCCCAAATTTGAGGACGCCCTATACATGCG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1175 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#24ATACCAGCTTATTCAATT-GCCCAATACGTATGTTGATACATGCCCCTTACCCTTTGCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1176 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#25ATACCAGCTTATTCAATT-CACCGTAGTAAGTAAGCAGACGCTAAGGATGTTGCCAGTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1177 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#26ATACCAGCTTATTCAATT-GTGACATGAACTGGTTTTCCACAGCTTAGGATCATGGATG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1178 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#27ATACCAGCTTATTCAATT-GGGCATGTGGGCTTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1179 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#28ATACCAGCTTATTCAATT-CCGTCGCTATAACCCTGTCTTTATATATCTTCGCCCACGGG-AGATAGTAAGTGCAATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1228 311835762019Surface-enhanced Raman spectroscopic single step detection of Vibrio parahaemolyticus using gold coated polydimethylsiloxane as the active substrate and aptamer modified gold nanoparticlesV.P. AptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellAptamer-based SERS method using Au-PDMS film for the detection of V. parahaemolyticus.The detection limit is 12 cfu/mL with a wide linear range from 1.2 × 10(2) to 1.2 × 10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1255 306086832019Visualized Detection of Vibrio parahaemolyticus in Food Samples Using Dual-Functional Aptamers and Cut-Assisted Rolling Circle AmplificationA-AptTACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCAAAAAAAAAA71N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellA biosensor using Dual-Apt and cut-assisted rolling circle amplification (CA-RCA) for rapid and visualized detection of Vibrio parahaemolyticus.Shows a good linear correlation in the range from 10(6) to 10 CFU/mL and LOD as low as 10 CFU/mL (g) in real food samples.N/AN/AN/ABiosensorN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1256 306086832019Visualized Detection of Vibrio parahaemolyticus in Food Samples Using Dual-Functional Aptamers and Cut-Assisted Rolling Circle AmplificationD-AptCCTCAGCATAAGCATGAATTGACCAACCTAAACTTATTCATTTTCCAGCACCTCTAATATTACTGGCGCAGTGCACCCACCCACCCACCC90N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellA biosensor using Dual-Apt and cut-assisted rolling circle amplification (CA-RCA) for rapid and visualized detection of Vibrio parahaemolyticus.Shows a good linear correlation in the range from 10(6) to 10 CFU/mL and LOD as low as 10 CFU/mL (g) in real food samples.N/AN/AN/ABiosensorN/A5'-Phosphate (3'-end phosphorylation)N/AN/AN/AN/AN/A
ABdb_1258 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA3PATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.Exhibits a wide linear detection range (from 10(2) to 10(7) cfu/mL), and the detection limit could be as low as 10 cfu/mL.N/AFlow Cytometry50.76 ± 8.92 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1259 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry32.53 ± 6.86 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1260 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA2GCAAAGAAACAGTGACTCGTTGA23N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry37.44 ± 6.98 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1261 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA3GCAACGAAACAGTGACTCGTTGA23N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry34.61 ± 3.47 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1262 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4CAACGAAACAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry28.65 ± 3.05 nMBiosensorWhole Cell-SELEX5'-FAM Labeled and 5'-Biotinylated (Biotin-TTTTTTTTT)N/AN/ABest CandidateN/AN/A
ABdb_1263 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA5AACGAAACAGTGACTCGTT19N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry422.0 ± 21.93 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1264 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA6ACGAAACAGTGACTCGT17N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry412.9 ± 27.12 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1265 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-1CAACGAAACATTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1004 ± 187.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1266 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-2CAACGAAACAGTTACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1103 ± 180.2 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1267 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-3CAACGAAACTGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry958.9 ± 183.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1268 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-4CAACGAAACAGCGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1070 ± 73.05 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1269 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-5CAACGAAACAGTGTCTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1119 ± 187.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1270 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-6CAACGAAATAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1070 ± 125.4 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1271 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-7CAACGAACCAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry423.2 ± 36.91 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1272 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-8CAACGATACAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry467.0 ± 33.38 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1273 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-9CAACGAAACAGTGAGTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry376.4 ± 54.25 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1384 329938652020A universal signal-on electrochemical assay for rapid on-site quantitation of vibrio parahaemolyticus using aptamer modified magnetic metal-organic framework and phenylboronic acid-ferrocene co-immobilized nanolabelAptamerTTTTTTTTTCAACGAAACAGTGACTCGTTG30N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a signal-on type electrochemical aptasensor (Fe3O4@NMOF-Apt) to rapidly and on-site detect V.P.Detects V.P in the range of 10–10(9) cfu/mL with an LOD of 3 cfu/mL and within only 20 min.N/ALinearized adsorption isotherm16.8 nM (with V.P.) and 2.2 ± 0.2 nM (with Fe3O4@NMOF-Apt)BiosensorN/A5'-Amidation (NH₂)N/ANo significant difference in the measured blank and signals was observed over 3 months with aptasensor, when 91.3% of the initial signal was obtained.N/AN/AN/A
ABdb_1385 329053292020Simple Colorimetric Assay for Vibrio parahaemolyticus Detection Using Aptamer-Functionalized NanoparticlesV.P. AptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a visual colorimetric assay using aptamer-conjugated MNPs and AuNPs for the detection of V. parahaemolyticus.Shows a linear range of 10-10(6) cfu/mL, with a limit of detection of 2.4 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) or ThiolatedN/AN/AN/AN/AN/A
ABdb_1444 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.01 (ID 5)TTTTTAAGCCCACAGACGWYCGGCAGGCACAGTYYGTCAAGGXCGYGCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitW = indole‐dU,Y = phenol‐dU,X = amine‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1445 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.02 (ID 6)TTTTTAAGCCCACCYCGCYGTGCAAGGCGAACGCCATCAGTGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitY = phenol‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1446 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.03 (ID 7)TTTTTAAGCCCACCYCCGWYCGAAGGCCACAGCYCATGCGCGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitY = phenol‐dU,W = indole‐dU, five additional Ts at the 5'-end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1447 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.04 (ID 8)TTTTTAACACGACCCCACYGTGCGAGCCGAACACCACCACGGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitY = phenol‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1448 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.05 (ID 9)TTTTTAACACGACXYAGCYGTGWGGGCCGAACACCAGGCACGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitX = amine‐dU,Y = phenol‐dU, five additional Ts at the 5'‐end, and CCATG at the 3′‐end, five additional Ts at the 5′‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1449 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.06 (ID 12)TTTTTAACACGACAGCAGWYCGGCGGGCACAGTGCGTGCGAGXCGYGCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.Aptamer ID 12 showed specific binding to Vp.2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitW = indole‐dU,Y = phenol‐dU,X = amine‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/ABest CandidateN/AN/A
ABdb_1450 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.07 (ID 13)TTTTTAACACGACCAWACYGTGGCAGACGAACGCCGTCACAGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitY = phenol‐dU,W = indole‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1451 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.08 (ID 14)TTTTTAAGCCCACGCGGCYGTGAGXCGCACAGCCAWAGCACGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitW = indole‐dU,Y = phenol‐dU,X = amine‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1452 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.09 (ID 15)GCCCACTGAACTGTGGCGGGCACAGGATGTGGAAGTGGGC40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitN/AN/AN/AN/AN/AN/A
ABdb_1500 320000582020A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrateApt 1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a SERS aptasensor using Apt-Au-PDMS film for simultaneous multiple-pathogen detection.Can selectively detect 18 cfu/mL cells.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1557 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA1TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG40N/AssDNAVibrio Parahaemolyticus (CICC 21617)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1558 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA3TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (CICC 21617)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1559 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA18PATAGGAGTCACGACGACCAGAAGCGGGCAGCTCCACCGGTAGGCTCCGAGTCATCACGGTCGTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (CICC 21617)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1567 337808522021Microfluidic thread-based electrochemical aptasensor for rapid detection of Vibrio parahaemolyticusAptamerATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a thread-based microfluidic nano-aptasensor based on MoS2 modified threaded electrodes for V. parahaemolyticus detection.The proposed aptasensor has a dynamic detection range of 10–10(6) CFU/mL, a detection limit of 5.74 CFU/mL, and an assay time of 30 min.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1569 334797972021A turn-on-type fluorescence resonance energy transfer aptasensor for vibrio detection using aptamer-modified polyhedral oligomeric silsesquioxane-perovskite quantum dots/Ti3C2 MXenes composite probesVP aptamerTTTTTTTTTCAACGAAACAGTGACTCGTTG30N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a turn-on–type aptasensor based on FRET pair using aptamer modified polyhedral oligomeric silsesquioxane-perovskite quantum dots (POSS-PQDs-Apt) as signal probe and titanium carbide (Ti3C2) MXenes as quencher for Vibrio parahaemolyticus (VP) determination.Under optimized conditions, the assay can determine VP in the concentration range 10(2) - 10(6) cfu/mL with the detection limit (LOD) of 30 cfu/mL and 100 cfu/mL by naked eye in aquaculture water.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1579 https://doi.org/10.1016/j.snb.2021.1303372021Electrochemical aptasensor for simultaneous detection of foodborne pathogens based on a double stirring bars-assisted signal amplification strategyApt-V.PTGGCTAGCTCAGTCATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTCCGCG60N/AssDNAVibrio ParahaemolyticusWhole cellDeveloped a double stirring bar-based signal-amplified strategy using aptamer-embedded tetrahedral DNA nanostructures for the simultaneous electrochemical detection of Vibrio parahaemolyticus (V.P) and Salmonella typhimurium (S.T).Display a lower detection limit of 4 CFU/mL for V.P. within a detection range of 10–10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1582 https://doi.org/10.1016/j.microc.2021.1061322021A novel photoelectrochemical aptamer sensor based on rare-earth doped Bi2WO6 and Ag2S for the rapid detection of Vibrio parahaemolyticusAptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a novel photoelectrochemical aptasensor based on rare-earth doped Bi2WO6 and Ag2S for the detection of V. parahaemolyticus.Showed a wide linear range from 3.2 × 10(2) CFU/mL to 3.2 × 10(8) CFU/mL, and a detection limit of 40 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AAfter 2 weeks of storage at 4°C in a refrigerator, the biosensor retained 95% of its initial response.N/AN/AN/A
ABdb_1745 https://doi.org/10.1016/j.snb.2021.1308392022A dual-mode aptasensor for foodborne pathogens detection using Pt, phenylboric acid and ferrocene modified Ti3C2 MXenes nanoprobeV.P-AptTTTTTTCACCCCACCTCGCTCCCGTGACACTAATGCTA38N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a dual-mode aptasensor based on electrochemical and colorimetric modes using PBA-Fc@Pt@MXenes nanoprobe for the detection of V.P in shrimps.Showed a LOD and linear range of 5 CFU/mL and 10(1) to 10 (8) CFU/mL by electrochemical mode and 30 CFU/mL and 10(2) to 10 (8) CFU/mL by coulometric mode.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AThe electrochemical signal retained 89.6% of its original value after 2 weeks.N/AN/AN/A
ABdb_1928 409166372025Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary ElectrophoresisAP1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped an aptamer-based capillary sieving electrophoresis (CE)-Laser-induced fluorescence (LIF) detection of three bacteria, Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus.The measured limit of detection (LOD) for V. parahaemolyticus was 3.70 × 10(6) CFU/mL and a linear range of 0.80-124 × 10(7) CFU/mL.N/AN/AN/ADetectionN/A5'-FAM LabeledN/AN/AN/AN/AN/A