Target Organism : Vibrio Cholerae

Total records found: 11

Download:

Search:

AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_1040	29408200	2018	Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA	CT916	CATCCGTCACACCTGCTC-GGCAAAAAGGATTGCCCAGGTCTGCTGTCTAGCCGGATTC-GGTGTTGGCTCCCGTATC	76	5'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'	ssDNA	Vibrio Cholerae	Cholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))	Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.	Detection limits of 2.1 ng/ml in buffer and 2.4 ng/ml in tap water, with a linear range from about 1 ng/ml to 1000 ng/ml.	12	Bead-based Affinity Chromatography	48.5 ± 0.5  nM	Biosensor	SELEX	Amidation (NH₂-(CH2)6) or Biotinylated and AlexaFluor 488 Labeled	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1041	29408200	2018	Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA	CT910	CATCCGTCACACCTGCTC-GTACACAGGAGCCAAACCCAACCAGACCAACCTGAAGCC-GGTGTTGGCTCCCGTATC	75	5'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'	ssDNA	Vibrio Cholerae	Cholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))	Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.	N/A	12	Bead-based Affinity Chromatography	54.6 ± 6.7 nM	Biosensor	SELEX	AlexaFluor 488 Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1042	29408200	2018	Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA	CT928	CATCCGTCACACCTGCTC-CACCCGTCACACTTATCCGTCAACTGGGCTCCCACTGGG-GGTGTTGGCTCCCGTATC	75	5'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'	ssDNA	Vibrio Cholerae	Cholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))	Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.	N/A	12	Bead-based Affinity Chromatography	23.6 ± 1.2 nM	Biosensor	SELEX	AlexaFluor 488 Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1043	29408200	2018	Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA	CT931	CATCCGTCACACCTGCTC-CCAGCGTGCGTCGAGGGGGACCCCTGTCAGCCCCCTCGCG-GGTGTTGGCTCCCGTATC	76	5'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'	ssDNA	Vibrio Cholerae	Cholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))	Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.	N/A	12	Bead-based Affinity Chromatography	50.2 ± 13.2 nM	Biosensor	SELEX	AlexaFluor 488 Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1044	29408200	2018	Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA	CT938	CATCCGTCACACCTGCTC-CACCCGTCCACTCATCCGTCACACCTGTTCTCCCCATG-GGTGTTGGCTCCCGTATC	74	5'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'	ssDNA	Vibrio Cholerae	Cholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))	Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.	N/A	12	Bead-based Affinity Chromatography	23.2 ± 0.4 nM	Biosensor	SELEX	AlexaFluor 488 Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1045	29408200	2018	Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA	CT939	CATCCGTCACACCTGCTC-CACCCGTCACACTTATCCGTCACACTGGGCCCCACTGGG-GGTGTTGGCTCCCGTATC	75	5'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'	ssDNA	Vibrio Cholerae	Cholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))	Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.	N/A	12	Bead-based Affinity Chromatography	41.6 ± 4.0 nM	Biosensor	SELEX	AlexaFluor 488 Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1046	29408200	2018	Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA	CT943	CATCCGTCACACCTGCTC-CACCCGTCCACTCATCCGACACACCTGCTCTCCCCACTG-GGTGTTGGCTCCCGTATC	75	5'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'	ssDNA	Vibrio Cholerae	Cholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))	Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.	N/A	12	Bead-based Affinity Chromatography	26.8 ± 0.5 nM	Biosensor	SELEX	AlexaFluor 488 Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1047	29408200	2018	Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA	CT946	CATCCGTCACACCTGCTC-CCCACAACCGGTCCGGTGTTCGGTCCCCGTATCACGCCCC-GGTGTTGGCTCCCGTATC	76	5'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'	ssDNA	Vibrio Cholerae	Cholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))	Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.	N/A	12	Bead-based Affinity Chromatography	30.4 ± 1.5 nM	Biosensor	SELEX	AlexaFluor 488 Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1494	32994896	2020	Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1	V.ch27	GCCTGTTGTGAGCCTCCTAAC-GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCAC-CATGCTTATTCTTGTCTCC	81	5'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'	ssDNA	Vibrio Cholerae O1 [Inaba (ATCC 39315) and Ogawa]	Whole cell	Identify aptamers and develop aptamer-nanobody-based ELISA for V. cholerae O1 detection.	The binding efficiency for V.ch27 was 53.3%.	12	Flow Cytometry	20.186 ± 3.655 pM	Detection	Whole Cell-SELEX	5'-FITC Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1495	32994896	2020	Aptamer-nanobody based ELASA for detection of Vibrio cholerae O2	V.ch47	GCCTGTTGTGAGCCTCCTAAC-CGTATTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTC-CATGCTTATTCTTGTCTCC	79	5'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'	ssDNA	Vibrio Cholerae O1 [Inaba (ATCC 39315) and Ogawa]	Whole cell	Identify aptamers and develop aptamer-nanobody-based ELISA for V. cholerae O1 detection.	Identify 10(4) CFU/ml with 25 pM of biotinylated aptamer and only 20 μg/ml of VHH without any cross-reactivity.	12	Flow Cytometry	15.404 ± 4.776 pM	Detection	Whole Cell-SELEX	5'-Biotinylated and 5'-FITC Labeled	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1952	11808691	2002	Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods	Anti-cholera whole toxin aptamers	N/A	N/A	5'-CCGGATCCGTTGATA-N20-GTGGTGTTGGCTCCC-3'	ssDNA	Vibrio Cholerae	Cholera toxin	Developed an aptamer-based electrochemiluminescence assay using a dsDNA aptamer for biotoxin detection.	Detection limit of less than 40 ng in the cholera electrochemiluminescence assay.	5	N/A	N/A	Biosensor	Magnetic Bead (MB)-based SELEX	5'-Biotinylated (for ECL) or 5'-Amidation (NH₂-(CH2)6) (for colorimetric assays)	N/A	N/A	N/A	N/A	N/A