AptBacterialDB

Target Organism : Streptococcus Mutans

Total records found: 32

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0396 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G6-25GGATCTGGTTAGTGAAGAGGGGGGAGGGGGGGTTGGGCTGTGGGGCGGTTAGTCGT56N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A6Flow CytometryN/ADetectionIn Silico Maturation (ISM)5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0397 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-5ATACATCTTAAGTCTCGTGGGGGGAGGGGGGGTTGGTGGGCTTT44N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)β-structure of membrane associated proteinImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.Colorimetric assay demonstrated detection of S. mutans cells at a concentration range of 1 × 10(5)-10(8) CFU/mL.11Flow Cytometry33 nMDetectionIn Silico Maturation (ISM)5'-ThiolatedN/AN/ABest CandidateN/AN/A
ABdb_0398 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-6ATACTATCGCATTCCTTCCGAGGGGGGAGGGGGGGGTGGGGGTCGGT47N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)β-structure of membrane associated proteinImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.Showed the highest binding signals to S. mutans with a 16-fold increase over SMa#3-6-P1.11Flow Cytometry33 nMDetectionIn Silico Maturation (ISM)5'-Fluorescein LabeledN/AN/ABest CandidateN/AN/A
ABdb_0399 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#3-6-P1ATACCAGCTTATTCAATTGGGGGGAGGGGGGGTTGGGCTGTGGGGCGGTTAGCCGC56N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A3Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0400 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-20CTACGTCTAGATTCCAGTCGAGGGGGGAGGGGGGGTTTTGGATCGGT47N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A11Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0401 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G10-11CAACCTGCTTATCTAAGGGGGGGAGGGGGGGTTGTGGGTAGGT43N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A10Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0402 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G2-1CTAGCAGTTCATTCAATAGGGGGGACGGGGGGTTAGGCTAGACTATGTCTAATCA55N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A2Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0403 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G5-5ATACACGTAAGTACCATTGGGCGGGGGGGGTGTTGTTTTCTACTATGTGCAATCG55N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A5Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0404 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-8ACACCACTTAATTCCATGCGAGGGGGGAGGGGGGGTTCGGGGACGGC47N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A11Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0602 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-1CGGATCCATGGGCACTATTTATATCAA-GCATCGGTCCTGAAGTTGCTCTAGTGCCCGTGTGCTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0603 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-2CGGATCCATGGGCACTATTTATATCAA-GGGCTAGCCCCGGATCACCACTTTCCCTGCTTGATGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0604 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-3CGGATCCATGGGCACTATTTATATCAA-TGTAACGGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0605 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-4CGGATCCATGGGCACTATTTATATCAA-TGGCACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0606 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-5CGGATCCATGGGCACTATTTATATCAA-CCTAACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0607 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-6CGGATCCATGGGCACTATTTATATCAA-CGGTTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0608 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-7CGGATCCATGGGCACTATTTATATCAA-GTGTTTCGCGCTATTTCCGTACAACCCGCGGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_1348 313182032019Engineering DNA-Nanozyme Interfaces for Rapid Detection of Dental BacteriaS. mutans-binding aptamerATACTATCGCATTCCTTCCGAGGGGGGAGGGGGGGGTGGGGGTCGGT47N/AssDNAStreptococcus Mutans (ATCC 25175)Whole cellDeveloped a DNA-engineered nanozyme interface using aptamers for the detection of dental bacteria.The detection limit is 12 CFU/mL, and the linear range is 0 to 10(9) CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1614 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateAptBHAAACCTGCTTATCTAAGGGGGGGAGGGGGGGTAGTGGGTGGGT43N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.AptBH-AgNPs at a concentration of 100 μg/mL after 48 h inhibited 43% of the biofilm formation and degraded 63% of the formed biofilm.N/AN/AN/ATherapeuticsN/A3'-BiotinylatedAgNPs-AptBH is weakly toxic, with cell viability of 96% even at 100 μg/mL. At 75 and 100 μg/mL, these concentrations caused ∼approximately 9% and 11% hemolysis, respectively.AgNPs-AptBH complex has not decomposed over time for 360 min in the cell culture medium.Best CandidateN/AN/A
ABdb_1615 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G10-11CAACCTGCTTATCTAAGGGGGGGAGGGGGGGTTGTGGGTAGGT43N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1616 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G11-6ATACTATCGCATTCCTTCCGAGGGGGGAGGGGGGGGTGGGGGTCGGT47N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1617 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G11-5ATACATCTTAAGTCTCGTGGGGGGAGGGGGGGTTGGTGGGCTTT44N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1618 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G11-20CTACGTCTAGATTCCAGTCGAGGGGGGAGGGGGGGTTTTGGATCGGT47N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1619 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G10-6ACACCAGCGTATTCTCTTGGGGGGAGGGGGGGTTGGGGGTCGGT44N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1620 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-1GCATCGGTCCTGAAGTTGCTCTAGTGCCCGTGTGCTCAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1621 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-2GGGCTAGCCCCGGATCACCACTTTCCCTGCTTGATGCAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1622 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-3TGTAACGGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1623 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-4TGGCACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1624 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-5CCTAACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1625 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-6CGGTTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1626 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-7GTGTTTCGCGCTATTTCCGTACAACCCGCGGACGCCTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1627 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateFAMGCAATGGTACGGTACTTCCCAAAAGTGCACGCTACTTTGCTAA43N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_2017 271512932016Identification of ssDNA aptamers specific to clinical isolates of Streptococcus mutans strains with different cariogenicityH19N/AN/A5'-GCAATGGTACGGTACTTCC-45N-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStreptococcus MutansWhole cellIdentify a high-affinity aptamer against cariogenic S. mutans strains.Aptamer H19 showed strong binding, as indicated by higher fluorescence intensity, with Strains 12 and 17 compared with other strains.9Flow Cytometry69.45 ± 38.53 nMDetectionSubtractive SELEX5'-FAM LabeledN/AN/AN/AN/AN/A