AptBacterialDB

Target Organism : Staphylococcus aureus (S. aureus)

Total records found: 292

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0001 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12b10_65GACGTCTTCGAATCCCCATACTGTGGCATTGGCTCAGGACGGTCTGGAGGATGGGGCTTGACGTG655'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)200 ± 20 (against short SEB peptide) and 420 nM (Full length SEB protein)DetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/ABest CandidateN/AN/A
ABdb_0002 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12b10TCAGCTGGACGTCTTCGAAT-CCCCATACTGTGGCATTGGCTCAGGACGGTCTGGAGGATGGGGCTTGACGTCATCATCTG-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0003 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12a10TCAGCTGGACGTCTTCGAAT-TACCAAGGTGTACGAGCGGCATTGGCTTAGGATGGTCTAGGAGAACGCCTTGTTCACGGG-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0004 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12d10TCAGCTGGACGTCTTCGAAT-GGGCATTGGCTAGGACGGTCTAGCAGGAGAAGATGTTAAGTGTGCCTTAGTCAGAGAGTG-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0005 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12e7TCAGCTGGACGTCTTCGAAT-GGGTATAGTGTCTGGCATTGGCGAGGATGGTCTCAAACGCTAAACCCAATCTGTATAACA-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0006 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BA8c3TCAGCTGGACGTCTTCGAAT-CGTGGGCATCGGGCTGCATGAATGGCATTGGCGAGGAGGGTGTGTTTGTAGGCAGCACCT-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0007 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BAE11a5TCAGCTGGACGTCTTCGAAT-ACCCGTACAGTCCTATGTTGGCATCCTCACTTGTGGCATTGGCAAGGATGGTCTTCTAG-TGTCAGGAGCTCGAATTCCC995'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0008 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BA8g1TCAGCTGGACGTCTTCGAAT-GCTCGACAGGGCACCACCGCCTCGGCATTGGCTAGGTTGGTCTCTTTGGCGGGGTTGCGC-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0049 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA20GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellIdentify aptamers that bind to S. aureus strains, including 8325-4, 196, and 04018, and develop a probe to distinguish them from S. epidermidis, Streptococcus, and E. coli.EC50 = 70.86 ± 39.22nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0050 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA23GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 = 61.50 ± 22.43nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0051 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA31GCAATGGTACGGTACTTCC-TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50=82.86 ± 33.20nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0052 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA34GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 =72.42 ± 35.23nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0053 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA43GCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 = 210.70 ± 135.91nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0132 224389272012A single-stranded DNA aptamer that selectively binds to Staphylococcus aureus enterotoxin BAPT(SEB1)GGTATTGAGGGTCGCATC-CACTGGTCGTTGTTGTCTGTTGTCTGTTATGTTGTTTCGT-GATGGCTCTAACTCTCCTCT785'-GGTATTGAGGGTCGCATC-N40-GATGGCTCTAACTCTCCTCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to SEB and quantification of all of the SEs in S. aureus contaminated food.Have an affinity and high selectivity for SEB.14N/AN/ADetectionSELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0133 224389272012A single-stranded DNA aptamer that selectively binds to Staphylococcus aureus enterotoxin BAPT(SEB2)GGTATTGAGGGTCGCATC-CCGTAGTGTGTTCTTATTCGTGTCTGTGTGTGTTCTGTCG-GATGGCTCTAACTCTCCTCT785'-GGTATTGAGGGTCGCATC-N40-GATGGCTCTAACTCTCCTCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to SEB and quantification of all of the SEs in S. aureus contaminated food.N/A14N/AN/ADetectionSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0139 221541692012Label-free detection of Staphylococcus aureus in skin using real-time potentiometric biosensors based on carbon nanotubes and aptamersNH2-AptamerGCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA single-walled carbon nanotube (SWCNT) functionalized with an aptamer-based biosensor to detect Staphylococcus aureus in real-time.With covalent functionalization, the minimum concentration detected was 8 × 10(2) CFU/mL; with the non-covalent approach, it was 10(7) CFU/mL.N/AN/AN/ADetectionN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0140 221541692012Label-free detection of Staphylococcus aureus in skin using real-time potentiometric biosensors based on carbon nanotubes and aptamersPyr-AptamerGCAATGGTACGGTACTTCCGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTATCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA single-walled carbon nanotube (SWCNT) functionalized with an aptamer-based biosensor to detect Staphylococcus aureus in real-time.With covalent functionalization, the minimum concentration detected was 8 × 10(2) CFU/mL; with the non-covalent approach, it was 10(7) CFU/mL.N/AN/AN/ADetectionN/A3'-C3-PyrN/AN/AN/AN/AN/A
ABdb_0142 224445662012Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella Typhimurium and Staphylococcus aureusAptamer2GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a luminescent bioassay for the simultaneous detection of S. Typhimurium and S. aureus using aptamer-conjugated MNPs and UCNPs (NaY0.78F4:Yb0.2, Tm0.02 UCNPs modified aptamer 1 and NaY0.28F4:Yb0.70, Er0.02 UCNPs modified aptamer 2).Display linear range of 10(1)-10(5) cfu/mL with a limit of detection of 8 cfu/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0236 236895052013Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticlesSA17TCCCTACGGCGCTAAC-CCCCCCAGTCCGTCCTCCCAGCCTCACACC-GCCACCGTGCTACAAC625'-TCCCTACGGCGCTAAC-N30-GCCACCGTGCTACAAC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC: 6538DR)Whole cellIdentify aptamers that binds to and recognize strains of S.aureus using aptamer-conjugated gold nanoparticles.LOD was 312 bacterial cells, and the lower limit of detection of the instrument was 63 ± 21 GNPs/μl for 100-nm particles and 508 ± 176 GNPs/μl for 60-nm particles.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)35 nM and 3.03 nM for SA17-GNPsBiosensorWhole Cell-SELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0237 236895052013Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticlesSA61TCCCTACGGCGCTAAC-CTCCCAACCGCTCCACCCTGCCTCCGCCTC-GCCACCGTGCTACAAC625'-TCCCTACGGCGCTAAC-N30-GCCACCGTGCTACAAC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC: 6538DR)Whole cellIdentify aptamers that binds to and recognize strains of S.aureus using aptamer-conjugated gold nanoparticles.LOD was 1250 bacterial cells, and the lower limit of detection of the instrument was 63 ± 21 GNPs/μl for 100-nm particles and 508 ± 176 GNPs/μl for 60-nm particles.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)129 nM and 9.9 nM for SA61-GNPsBiosensorWhole Cell-SELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0263 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA1AGCAGCACAGAGGTCAGATG-AGGCGATTACGCTTCTTGTACTTCAATAACGACTCAACTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy92.17 ± 16.75 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0264 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA15/40AGCAGCACAGAGGTCAGATG-TACTTATGCATTTCCTCCCACGATCTTATTTGAGAGTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.Limit of detection of 8.7±10(-3) μg/mL with linear range of 0.01-10 μg/mL SEA.12Fluorescence Spectroscopy48.57 ± 6.52 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0265 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.2AGCAGCACAGAGGTCAGATG-ATGATCGTAGTCATTTAAAATTTGAATACTATCAAAGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy235.00 ± 79.05 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0266 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA3AGCAGCACAGAGGTCAGATG-TACGAGCGGTGGTTTTACCCTGCAATACTTTTGGCTGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0267 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA25AGCAGCACAGAGGTCAGATG-ATAGATTTTTTTCATGATTCTTCATTTTTTTATTTGAAAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0268 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA29AGCAGCACAGAGGTCAGATG-TATTATCTTACTACGTTGCATCCTACTTTTATAGTCCCCT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0269 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA30AGCAGCACAGAGGTCAGATG-GGATCTTCCCTCAATGTTTATTGTATATCTGTACTCGTAA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0270 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA10AGCAGCACAGAGGTCAGATG-TCCAATTCAATTCATTTCTGAACTTAGTCGGCACTTTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0271 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA4AGCAGCACAGAGGTCAGATG-CTCCAGCAGCCTCATAGATACGTCGTATCTATTGTTTCCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0272 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.1AGCAGCACAGAGGTCAGATG-AAGGTCTTACATCAATTTATATATTTTTCCAATATCTGTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0275 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #2GGGAGAGCGGAAGCGUGCUGGGCC-GGGAGUUUUGAUACGGCUUCAUGCAGUAAUGUUUUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.The level of binding of #2 RNA aptamer to S. aureus was 1.6-fold higher than that of the control aptamer.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/ABest CandidateN/AN/A
ABdb_0276 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #10GGGAGAGCGGAAGCGUGCUGGGCC-GUUAAUACGGUGUCUUUUUCGGUCGUGUAUAAAACGGAAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0277 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #14GGGAGAGCGGAAGCGUGCUGGGCC-UAGGACAGUUCGUCCUCAUUACAUCGCCGCCUAACACAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0278 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #16GGGAGAGCGGAAGCGUGCUGGGCC-UUAGAAGUAGCCUGCUACGCAUGGUCGACUCAAGAAUCG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0279 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #18GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0280 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #21GGGAGAGCGGAAGCGUGCUGGGCC-AGUCUGACACGUAGACAGUUCUAUUACUUACGUCGAGA-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0281 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #23GGGAGAGCGGAAGCGUGCUGGGCC-ACAGUGUUCUAAUGCGACAAUGGAGUCUGUGGCAAAGUGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0282 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #24GGGAGAGCGGAAGCGUGCUGGGCC-UCUCAGGCCGACAUUCUGAGAACGCGAGGCGUAUUGAAG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0283 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #26GGGAGAGCGGAAGCGUGCUGGGCC-UUCAAGUAGGGGCGGUUUACUAUCUGGAUCUUGUAGUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0284 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #07GGGAGAGCGGAAGCGUGCUGGGCC-ACUUGGGGACGACGAGUAGAUAGUAAGGUGGAGACCUGGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0285 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #06GGGAGAGCGGAAGCGUGCUGGGCC-UAUGACAUAAGGUGGGCUGGGAAGCUAGAGCAUGUAAGG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0286 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #05GGGAGAGCGGAAGCGUGCUGGGCC-GUGAAGAAAAAGGGGCGGAUUGGGUAGUAGGGAGGAGAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0287 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #04GGGAGAGCGGAAGCGUGCUGGGCC-AGGAUAGGGGAUGAAGAAAAAAAGAAGGGUGCCGUGGCGC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0288 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone G1GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0335 238720602013Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteriaA3GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CECT 4630)Type IVB Pili structural proteinsDevelop a potentiometric biosensor based on carbon nanotubes (transducer layer) and aptamers (sensing layer) for the detection of bacteria.Display linear range from 8 × 10(2) CFU/mL–10(8) CFU/mL with LOD of 8 x10(2) CFU/mL and the signal was stable for at least one hour.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0339 239293942013A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detectionS. aureus (FASA)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped an aptamer-functionalized graphene oxide (GO) nano-biosensor for multiplexed pathogen detection.Detection limits (LOD) of 800.0 cfu/mL (S. aureus) and linear ranges of 10(4)–10(6) cfu/mL.N/AN/A35 nMBiosensorN/A5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0356 251855032014Selection of peptidoglycan-specific aptamers for bacterial cells identificationAntibac1TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC555'-TCGCGCGAGTCGTCTG-N24-CCGCATCGTCCTCCC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923) and Escherichia Coli (E. Coli) TOP10PeptidoglycanIdentify aptamers that bind to the peptidoglycan of bacterial cells.High binding affinity for S. aureus, as the radioactivity of Antibac1 was 28 times higher than the negative control.5Saturation Binding Assay0.415 + 0.047 μMDetectionSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0357 251855032014Selection of peptidoglycan-specific aptamers for bacterial cells identificationAntibac2TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC555'-TCGCGCGAGTCGTCTG-N24-CCGCATCGTCCTCCC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923) and Escherichia Coli (E. Coli) TOP10PeptidoglycanIdentify aptamers that bind to the peptidoglycan of bacterial cells.High binding affinity for S. aureus, as the radioactivity of Antibac2 was 22 times higher than the negative control.5Saturation Binding Assay1.261 + 0.280 μMDetectionSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0414 243259832014Graphene-based potentiometric biosensor for the immediate detection of living bacteriaSA20GCAATGGTACGGTACTTCCGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTATCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CECT 4630)Whole cellDeveloped a potentiometric aptasensor based on graphene oxide (GO-covalently conjugated aptamer) and reduced graphene oxide (RGO-non-covalently conjugated aptamer) to detect S. aureus.Able to detect 1 CFU/mL in a few minutes.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0415 243259832014Graphene-based potentiometric biosensor for the immediate detection of living bacteriaSA31GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CECT 4630)Whole cellDeveloped a potentiometric aptasensor based on graphene oxide (GO-covalently conjugated aptamer) and reduced graphene oxide (RGO-non-covalently conjugated aptamer) to detect S. aureus.Able to detect 1 CFU/mL in a few minutes.N/AN/AN/ABiosensorN/A3'-C3-PyrN/AN/AN/AN/AN/A
ABdb_0416 248045562014Aptamer-fluorescent silica nanoparticles bioconjugates based dual-color flow cytometry for specific detection of Staphylococcus aureusAptamerGCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDevelop aptamer recognition and FSiNPs label-based dual-colour flow cytometry assay (Aptamer/FSiNPs-DCFCM) for the detection of S. aureus.Detects as low as 1.5×10(2) and 7.6×10(2) cells/mL S. aureus in buffer and spiked milk, respectively.N/AN/AN/ABiosensorN/A5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0430 245686252014Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labelsApt₁GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a multiplex method for the simultaneous detection of three pathogenic bacteria using multicolour upconversion nanoparticles (UCNPs) coupled with aptamers.The linear range showed from 50-10(6) cfu/mL, and the limits of detection were 25 cfu/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0457 249138712014A sensitive gold nanoparticle-based colorimetric aptasensor for Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a gold nanoparticle-based colorimetric aptasensor for S. aureus using tyramine signal amplification (TSA) technology.Exhibited a linear detection range from 10 to 10⁶ cfu/mL with a limit of detection (LOD) of 9 cfu/mL.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0538 256243252015Neutralization of staphylococcal enterotoxin B by an aptamer antagonistA11ATACCAGCTTATTCAATT-TAGGCGGTGGAGATAGTAAGTGCAATCTAGGCGGTGGAGATAGTAAGTGCAATCTATGCC-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and block SEB-mediated toxic shock (TSS) in vitro.Inhibited SEB-mediated PBMC proliferation of 93% and TNF-α, IL-1β, IL-6, IL-2, and IFN-γ in culture supernatants were reduced by 77, 97, 64, 96, and 99%, respectively. 90% survival of mouse models of SEB-induced TSS.11Fluorescence Spectroscopy64 nMTherapeutics/ImmunmodulatorySELEX5′-Polyethylene glycol (PEG) with 5'-Amidation (NH₂) and 3’-Inverted Thymidine (3'-idT) and 5'-Biotinylated or 5'-FITC LabeledShowed no cytotoxic effects on PBMCs.N/AN/AN/AN/A
ABdb_0539 256243252015Neutralization of staphylococcal enterotoxin B by an aptamer antagonistA2ATACCAGCTTATTCAATT-GATCGATCGATCAGTCAGTCGACCAACGCACGACACGCATCGCTCTATCTCGTCGAACAG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and block SEB-mediated toxic shock (TSS) in vitro.A2 achieved 34% PBMC proliferation inhibition.11Fluorescence Spectroscopy26 nMTherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0540 258914722015Antibiotic loaded nanocapsules functionalized with aptamer gates for targeted destruction of pathogenSA20hpGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAptamer-gated nanocapsules for the specific targeting of vancomycin to bacteria for the controlled release of vancomycin.15-fold increase of efficacy for vancomycin with MIC of 0.420 μg/mL.N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-AlexaFluor 488 Labeled and 3'-(PEG)-AGGGCGC-BH1MICs of vancomycin-nanoparticles for S. epidermidis (control) were found to be 6.295 µg/mL.N/AN/AN/AN/A
ABdb_0541 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1C10AGCAGCACAGAGGTCAGATG-TATACTTCTAAAATTTGTTTGTATCTACGATGTTCTTCGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.Have a limit of detection of 6 ng/mL with a range of 0.01–10 μg/mL of SEC1.12Fluorescence Spectroscopy65.14 ± 11.64 nMBiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0542 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C2C36.2AGCAGCACAGAGGTCAGATG-TATCAGAATTAATGCTCTCGTAATTTTCGAATCGTCGTGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence Spectroscopy49.43 ± 11.76 nMBiosensorSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0543 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C3C34.1AGCAGCACAGAGGTCAGATG-CTTTCATTTTTATTCTTTTTGACTGTATTTTATGTACCAT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0544 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C4C4AGCAGCACAGAGGTCAGATG-CTAACCTAATTCGACCGTGTATTCTTCTGCGTTATTTACC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0545 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C5C10AGCAGCACAGAGGTCAGATG-TATACTTCTAAAATTTGTTTGTATCTACGATGTTCTTCGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0546 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C6C9AGCAGCACAGAGGTCAGATG-TCCATTATCAGGTTCTTTATTCTGTTGTTCAACTTATTAA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence Spectroscopy154.9 ± 45.67 nMBiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0547 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C7C32.1AGCAGCACAGAGGTCAGATG-TTTTAGATTTAGCTCTTATTTGTTCGAGCAATCCCAAAGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0548 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C8C2AGCAGCACAGAGGTCAGATG-GCCAACTCAATTTTTTGTTTTGTCTTTCCGATTGATCTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0549 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C9C6AGCAGCACAGAGGTCAGATG-CATATCGAATTTTCCTCGTGATTTTTCTTTCATTCTTAGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0550 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C10C11AGCAGCACAGAGGTCAGATG-TAGTTAATGGATTTTTCTTCTTTATCTTCTTATTTCTTGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0551 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C11C30AGCAGCACAGAGGTCAGATG-ATGTTTTCCCTTATCACTACTTTTATTTGTCTTTTTGCAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0552 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12UGUAAUUCUGCCAUUCUUUUUGGGGCGGAAUACAGGAUGU405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.fmA12-functionalized AgNPs resulted in specific S. aureus antimicrobial activity with only a 1.6 ± 1.0% survival of 12598 cells and 51.9 ± 2.0% survival of 10832 cells.8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)95 nM (by SPR) and 67.1 ± 6.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/A76% fmA12 survives in 10% mouse serum at 37°C over 24 h, and in 50% serum, 85.0% fmA12 survives after 5 h.N/A~52 h for fmA12.N/A
ABdb_0553 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmF07AUACGCUGCUCACUAAAGUCGGGAGUACUCCAAUGUUUUC405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)38.9 ± 4.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/ABest CandidateN/AN/A
ABdb_0554 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmE09AUCUAGCAAGCACUUGCAAACAUUCGAUAACGGGGAUUC395'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)418 ± 112 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0555 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmG12UGCCAAUGUAACUCAACACUCUUCAUUGAGCUGUCGGGCA405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)475 nM (by SPR) and 220 ± 24 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0556 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ3N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.7 ± 3.5 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0557 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ6N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)63.1 ± 7.3 μMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0558 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ9N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.1 ± 16.7 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0566 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGGCGTGGGAGGCAATGCCTTGCTTGTAGGCTTCCCCTGTGCGCG-GGTGGATCCATATTCCTACTCG1075'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0567 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA14CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT-GGTGGATCCATATTCCTACTCG1085'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence Spectroscopy3.49 ± 1.43 nMDetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0568 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA15CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACGCGCAAACAGATTAACACTCCGCCTAAGTCTGCCGCACGC-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0569 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA20CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCAGCGGGGGCTGCGCGGTGGAGTGCTGTGGGCG-GGTGGATCCATATTCCTACTCG985'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0570 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGAGCCAGGATGGGAGGTCTGTAGGTCTGCGGGGCGTG-GGTGGATCCATATTCCTACTCG1045'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0571 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB6CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGTCGGTGTCTGCCGGGGGATGTGGAGGCTGGGTGTTGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0572 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB7CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGGGCGGGCTACCTGGCTAGTACGCCATGATGCCTGCACGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0573 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB15CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACGCGCAAACAGATTAACACTCCGCCTAAGTCTGCCGCACGC-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0574 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/8ATACCAGCTTATTCAATT-AGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.PA#2/8 competes with IgG or IgG-Fc for binding to Protein A.11Bead-based Binding Assay1.06 ±0.2 μMDetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0575 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/22ATACCAGCTTATTCAATT-GCAGTACTGATGAGTGTAGCCGTATGATTATCGTTTGTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0576 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/34ATACCAGCTTATTCAATT-CCCCAACGAGTCGATATGTAGCCCACACTCTGATTCGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0577 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/11ATACCAGCTTATTCAATT-GGAGACGACAAACTATTACGTACTACGGCATGCACTTGGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0578 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/3ATACCAGCTTATTCAATT-CGACAAGTGGGCATTACGATTCTAGCCCTGATTATGTTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0579 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/6ATACCAGCTTATTCAATT-ACCGATCACTAGCCGACTAATTGGTTTCCGATCGCAGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0580 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#14/82ATACCAGCTTATTCAATT-CCACAACCGAACTCGTAAGACGTATGTAGCCGCCAACTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0581 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/52ATACCAGCTTATTCAATT-ACGCATTGGAGCCCGAAACTGATTCATTGAGCCTACCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0582 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/14ATACCAGCTTATTCAATT-ACGACCGTAGACGACTTACACTGATGTTGCGCATTTCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0583 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/31ATACCAGCTTATTCAATT-CGATGACGACTGTAGCCGCAATACGCCCCTGTTACGTTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0584 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/41ATACCAGCTTATTCAATT-GGACGCCGACTAACTTTACGTGGTTCTCCTACCGCCTAACC-ACAATCGTAATCAGTTAG775'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0585 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/43ATACCAGCTTATTCAATT-ACGAAATGTAGCCGATCCTGATTACTCTCTGTCAGCTTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0626 255627422015Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of staphylococcus aureusPrimary anti-S.aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDual-aptamer-based sandwich immunosensor (Apt/S.aureus/apt-AgNP) for the detection of S. aureus.Display a dynamic range from 10 to 1×10(6) cfu/mL with a low detection limit of 1.0 cfu/mL.2N/A35 nMBiosensorWhole Cell-SELEX5'-Biotinylated (Biotin-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0627 255627422015Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of staphylococcus aureusSecondary anti-S.aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDual-aptamer-based sandwich immunosensor (Apt/S.aureus/apt-AgNP) for the detection of S. aureus.Display a dynamic range from 10 to 1×10(6) cfu/mL with a low detection limit of 1.0 cfu/mL.2N/A129 nMBiosensorWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0644 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.06TGTACCGTCTGAGCGATTCGTAC-GATTACTATAATTTCCTATCGTCCGACCGCCGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.Display a sensitive detection of 200 nM alpha toxin.12Surface Plasmon Resonance (SPR)93.7 ± 7.0 nMDetectionSELEX5'-Amidation (NH₂-(CH2)6) or 5'-FAM LabeledN/AN/AN/AAverage half-life of ssDNA MREs in serum is about one hour.N/A
ABdb_0645 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.26TGTACCGTCTGAGCGATTCGTAC-CCTTGCCGATGCCTTTACGGTCTAGTTTGGATGT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0646 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.01TGTACCGTCTGAGCGATTCGTAC-TCGGGCGATGATACTTAGCACGGTCTAGGTCAAA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0647 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.20TGTACCGTCTGAGCGATTCGTAC-TAGCGGCAGAGTAGCACTCTATAGGTCGATGTTT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0648 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.02TGTACCGTCTGAGCGATTCGTAC-CGTGTCCTATTTTCTTCTCTGTTAACTCTCGTC-AGCCAGTCAGTGTTAAGGAGTGC795'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0649 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.06TGTACCGTCTGAGCGATTCGTAC-GATTACTATAATTTCTTATCGTCCGACCGCCGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0650 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.39TGTACCGTCTGAGCGATTCGTAC-TTTGATCTCGTGTGTCTAGTTGCGGCGGATTGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0651 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.10TGTACCGTCTGAGCGATTCGTAC-GGTCAACCTCACCGACTGCCGACCGTTTAATTCG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0652 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.43TGTACCGTCTGAGCGATTCGTAC-CGTCATTGCCTCGTAGTATTCTTATAGTCGGTAG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0653 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.44TGTACCGTCTGAGCGATTCGTAC-TCCCGAAAGCGCGTCAGCCTGGGAGGTTATGCGG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0696 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA20GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 70.86 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0697 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA23GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 61.50 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0698 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA34GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 72.42 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0699 254611752015A new aptamer/graphene interdigitated gold electrode piezoelectric sensor for rapid and specific detection of Staphylococcus aureusS. aureus aptamer.GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped an aptamer/graphene interdigitated gold electrode piezoelectric sensor to detect Staphylococcus aureus.Achieved a detection limit of 41 cfu/mL with a linear range of 4.1×10(1) to 4.1×10(5) cfu/mL. The detection is rapid, completing in less than 60 minutes.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0701 262417352015Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA GNPs-modified Raman-based biosensor was developed for the simultaneous detection of S. typhimurium and S. aureus.Linear detection range of 10^2 to 10^7 cfu/mL for both; LOD of 35 cfu/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0703 https://doi.org/10.1080/00032719.2015.10362782015Aptamer Immobilized Magnetoelastic Sensor for the Determination of Staphylococcus aureusS. aureus aptamer.GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDevelop an aptamer-based magnetoelastic sensor for the detection of S. aureus.Linear dynamic range of 10^1–10^11 cfu/mL and detection limit of 5 cfu/mL.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0756 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS3ATACCAGCTTATTCAATT-CCCGCCTCTGAGCATTATTAATGTTATACCTTACGGCTGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.Inhibited PBMCs proliferation with an inhibition rate of 84% and reduced IFN-γ, TNF-α, IL-2, and IL-6 secretions by 89%, 87%, 73%, 69%, respectively. PEGS3 allowed 79% of the mice to survive the lethal challenge.9Enzyme-Linked Aptamer Assay (ELAA)36.93 ± 7.29 nMTherapeutics/ImmunmodulatorySELEX5′-Polyethylene glycol (PEG) with 5'-Amidation (NH₂) and 3’-Inverted Thymidine (3'-idT) and 5'-Biotinylated or 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0757 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS6ATACCAGCTTATTCAATT-GCCGCGGAATTGTGGCTTGGTGTCGTGGTCAGGGGCTGG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.N/A9Enzyme-Linked Aptamer Assay (ELAA)N/ATherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0758 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS12ATACCAGCTTATTCAATT-GCCACTGTGGACTTGTTATTCGTCTTTGCCGTCTAATTCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.Inhibited PBMCs proliferation with an inhibition rate of 84%.9Enzyme-Linked Aptamer Assay (ELAA)79.35 ± 12.09 nMTherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0759 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS23ATACCAGCTTATTCAATT-GCAACAATGTTGTGTCAGCAAGTTGGTTGGCGGTGTATTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.N/A9Enzyme-Linked Aptamer Assay (ELAA)N/ATherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0760 https:doi.org10.1016j.carbon.2016.04.0142016Biodegradable graphene oxide and polyaptamer DNA hybrid hydrogels for implantable drug deliveryPolyaptamer (PA)ATCGGCTAGCACGTACAGAACTAAAAAAAAAAAA-GTCGGCTTAGCCTCAACCCCC-AAGGCAAAAAGGCAT70N/AssDNAEscherichia Coli (E. Coli) and Staphylococcus aureus (S. aureus)Kanamycin (Kan)Kan-loaded PA-GO (Kan/PA-GO) hybrid hydrogels for antibacterial effects.Significantly reduced the viability of E. coli and S. aureus to 24.2 ± 4.8% and 17.7 ± 0.7%, respectively.N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-PhosphateN/AN/AN/AN/AN/A
ABdb_0784 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8ATACCAGCTTATTCAATT-AGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.PA#2/8 and its truncated variant PA#2/8[S1-58] were able to differentiate between the highly Protein A-producing S. aureus Cowan strain.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)101.4 ± 5.9 nM (of 3′-biotinylated)  and 189.9 ± 13.0 nM (of 5′-biotinylated)DetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0785 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT585'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.PA#2/8 and its truncated variant PA#2/8[S1-58] were able to differentiate between the highly Protein A-producing S. aureus Cowan strain.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)11.3 ± 1.4 nM (of 3′-biotinylated)  and 23.7 ± 2.0 nM (of 5′-biotinylated)DetectionN/A5'-and 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0786 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-50]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTC505'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0787 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-43]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGG435'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0788 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S28-50]AGGGGGATAGAGGGGGTGGGTTC235'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0796 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC2TGCAGGATCCGGTATCCGTGGACGGTGTGCAGGATCCGGTATCCGTGGGCACGAGAATTCCTCCGTTGCG705'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.LOD in the minimum quantity of 5 ng SEB per 100 µl of human serum.12Enzyme-Linked Immunosorbent Assay (ELISA)2.3 × 10(−11) MBiosensorSELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0797 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC3TGCAGGATCCGGTATCCGTGCACACACACCCAACAACCAGCTGCCGCACCGGAGGAATTCTCGT645'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0798 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC7TGCAGGATCCGGTATCCGTGGGCGGGGCCATGCAGGATCCGGTATCCGTGGGCCGCAACGGAGGAATCTCGT725'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0799 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC8CCGTGGCCGCAACGGAGGAATTCTCGT275'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0800 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC9ACGAGAATTCCTCCGTTGCGGCACAGTTGGGCAGGAACCTGTGGGGCGTGCGCAACGGAGGAATTCTCGT705'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0801 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC10ACGAGAATTCCTCCGTTGCGGCCCACGGATACC335'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0802 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC11TGCAGGATCCGGTATCCGTGCGCAACGGAGGAATTCTCGT405'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0803 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC12ACGAGAATTCCTCCGTTGCGGCCCACGGATACAGGATCCTGCATGCCTGTCCACGGATACCGGATCCTCA705'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0804 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC17TGCAGGATCCGGTATCCGTGCCAAACACACCAAAAGCCCCCCCAACCCACAACCACGTCC605'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0805 273185662016Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugatesAptamerGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAptamer-functionalized silica magnetic nanoparticles-based assay for on-site determination of S. aureus.Detects S. aureus cells as low as 682 CFU in whole blood, with a linear range from 800 CFU/ml blood to 104 CFU/ml blood.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0809 274117752016Rapid and Selective Detection of Pathogenic Bacteria in Bloodstream Infections with Aptamer-Based RecognitionApt-S. aureusTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAACTTTTTTTTT71N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAptamer-based capture platform using Fe3O4@mTiO2 magnetic nanoparticles for the detection of bacteria.Display a favourable bacterial-capture efficiency of about 80% even at low infectious doses (10–2000 CFU/mL).N/AN/AN/ADetectionN/A3'-Amidation (NH₂-(CH2)7) and 3'-FAM Labeled ((CH2)7-FAM)N/AN/AN/AN/AN/A
ABdb_0812 266411082016Dual Recognition Strategy for Specific and Sensitive Detection of Bacteria Using Aptamer-Coated Magnetic Beads and Antibiotic-Capped Gold NanoclustersSA-aptamerGCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellAptamer-coated magnetic beads (Apt-MB) and Van-functionalized fluorescent gold nanoclusters (AuNCs@Van) were employed for specific capture of SA.Sensitive quantification of SA in the range of 32–10(8) cfu/mL with the detection limit of 16 cfu/mL via fluorescence intensity.N/AN/AN/ABiosensorN/A5′-Biotinylated and 5′-Cyanine3 (Cy3) and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0813 274275912016Duplex Identification of Staphylococcus aureus by Aptamer and Gold NanoparticlesSA23GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA45N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDevelop an aptamer-AuNPs-based colorimetric method for duplex identification of S. aureus.AuNPs as a sensor could well distinguish S. aureus from S. enteritidis and P. mirabilis.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0817 269712742016Dual-excitation upconverting nanoparticle and quantum dot aptasensor for multiplexed food pathogen detectionS. aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDual-excitation aptasensing platform based on the luminescent nanoparticles (QDs and UCNPs) for the detection of Salmonella typhimurium and Staphylococcus aureus.Linear detection range: 50 to 10⁶ cfu/mL; LOD: 16 cfu/mL (S. aureus).N/AN/A35 nMBiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0918 281195142017High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis AgentsAntibac1TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC55N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922), Klebsiella Pneumoniae (ATCC 27853), Proteus Mirabilis (ATCC 00557), Pseudomonas Aeruginosa (ATCC 700603), Staphylococcus aureus (S. aureus) (ATCC 29213), Streptococcus Pneumoniae (ATCC 23855), and Enterococcus Faecium (ATCC 29212), and Proteus Vulgaris, Morganella Morganii, Citrobacter Freundii, and Acinetobacter BaumanniiPeptidoglycanDevelop generic probes as biological recognition elements in biosensors for sepsis diagnosis in the clinical setting.Antibac1 bound with higher efficiency to both gram-positive (S. aureus, S. penumoniae, L. monocytogenes) and gram-negative (A. baumannii, C. freudii, K. pneumoniae, P. aeruginosa, P. mirabilis).N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)268.50 ± 54.34 nM (for A. baumannii); 51.74 ± 11.75 nM (for L. monocytogenes); 31.82 ± 4.38 nM (for E.coli); 170.10 ± 32.13 nM (for S.aureus) and 256.10 ± 47.89 nM (for K. pneumoniae)DiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_0919 281195142017High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis AgentsAntibac2TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC55N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922), Klebsiella Pneumoniae (ATCC 27853), Proteus Mirabilis (ATCC 00557), Pseudomonas Aeruginosa (ATCC 700603), Staphylococcus aureus (S. aureus) (ATCC 29213), Streptococcus Pneumoniae (ATCC 23855), and Enterococcus Faecium (ATCC 29212), and Proteus Vulgaris, Morganella Morganii, Citrobacter Freundii, and Acinetobacter BaumanniiPeptidoglycanDevelop generic probes as biological recognition elements in biosensors for sepsis diagnosis in the clinical setting.Antibac2 bound with higher efficiency to the gram-positive species S. aureus and E. faecium and to the gram-negative species (A. baumannii, C. freudii, K. pneumoniae, M. moraganii, P. aeruginosa, P. mirabilis, and E. coli).N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)71.92 ± 9.74 nM (for A. baumannii); 54.19 ± 12.09 nM (for L. monocytogenes); 62.43 ± 11.97 nM (for E.coli); 194.90 ± 38.55 nM (for S.aureus) and 195.90 ± 42.91 nM (for K. pneumoniae)DiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_0953 291608512017Development of An Impedimetric Aptasensor for the Detection of Staphylococcus aureusPA#2/8[S1-58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) (DSM 20231)Staphylococcus aureus Protein A (SpA)Developed an impedimetric aptasensor for the detection of S. aureus.Showed a limit of detection of 10 CFU/mL within 10 minutes.N/AElectrochemical impedance spectroscopy (EIS)18.5 ± 1.8BiosensorN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0956 288034752017Intuitive Label-Free SERS Detection of Bacteria Using Aptamer-Based in Situ Silver Nanoparticles SynthesisS.aureus-AptamerSTCCCTACGGCGCTAACCTCCCAACCGCTCCACCCTGCCTCCGCCTCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (CICC 21600)Whole cellDeveloped a SERS-based aptasensor using aptamer@AgNPs for the detection of bacteria.Exhibited a linear concentration, ranging from 10(1) to 10(7) cfu/mL with the detection limit of 1.5 cfu/mL.N/AN/AN/ABiosensorN/AFAM LabeledN/AN/AN/AN/AN/A
ABdb_0965 282877152017Dual-Recognition Förster Resonance Energy Transfer Based Platform for One-Step Sensitive Detection of Pathogenic Bacteria Using Fluorescent Vancomycin-Gold Nanoclusters and Aptamer-Gold NanoparticlesAptamerGCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Bacterial cell wallDevelop a dual-molecular FRET platform based on the recognition of bacterial cell walls by antibiotic and aptamer molecules, respectively, for the detection of pathogenic bacteria.Shows a linear concentration of Staph. aureus in the range from 20 to 10(8) cfu/mL with a detection limit of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated and Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0974 279514522017(1→3)-β-D-glucan aptamers labeled with technetium-99m: Biodistribution and imaging in experimental models of bacterial and fungal infectionSeq6CGACTGACGCCTCCAAGCCACACGTGTGAGAAGGGTGTTATCATGTATTTCGTGTTCCTTTCGTCATTCCTTTGTCTGCATGCATCGCTACGTG94N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)(1 → 3)-β-D-glucansRadiolabeled aptamers were evaluated to identify infectious foci caused by bacterial cells.The target/non-target ratios were 3.17 ± 0.22 for seq6 in S. aureus-infected animals.N/AN/A0.303 ± 0.067 μMImagingN/A5'-Radiolabelled (99mTc labeled)N/AIn vitro stability of seq6 aptamer labelled with 99mTc in the presence of 0.9% saline, plasma, and cysteine excess (molar ratio) was above 84.2 ± 2.9% in all cases at 24hrs.N/AN/AN/A
ABdb_0975 287158742017Detection of bacterial infection by a technetium-99m-labeled peptidoglycan aptamerAntibac1TCGCGCGAGTCGTCTGGGGACAGGGAGTGCGCTGCTCCCCCCGCACGTCCTCCC54N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)PeptidoglycanRadiolabeled aptamers were evaluated for biodistribution studies and scintigraphic imaging in infection‐bearing mice.Achieved a high target-to-non-target (T/NT) ratio of 4.7 ± 0.9 at 1.5 h and 4.6 ± 0.1 at 3.0 h in bacterial-infected models.N/ABinding Assay0.170 ± 0.032 μMImagingN/A5'-Inverted Thymidine (5'-idT), 3'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (99mTc labeled)N/ARadiolabeling yields were superior to 90% and 99mTc‐Antibac1 was highly stable in the presence of saline, plasma, and cysteine up to 6 h.N/ADistribution half-life: 6.7 min and Elimination half-life: 41.3 min.N/A
ABdb_0980 286917952017Copper-Based Metal-Organic Framework Nanoparticles with Peroxidase-Like Activity for Sensitive Colorimetric Detection of Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA colorimetric method for the detection of S. aureus was developed using aptamer-modified Cu-MOF nanoparticles.The linear range for S. aureus detection is 50-10,000 CFU/mL, with a detection limit of 20 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0981 281591082017An enhanced chemiluminescence resonance energy transfer aptasensor based on rolling circle amplification and WS2 nanosheet for Staphylococcus aureus detectionAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellA chemiluminescence resonance energy transfer aptasensor was developed for the detection of S. aureus with Co2+ enhanced N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functional flowerlike gold nanoparticles (Co2+/ABEI-AuNFs) and WS2 nanosheet.The CL signal was found to be linear within the range of 50 cfu/mL to 1.5 × 10(5) cfu/mL, and the limit of detection was 15 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1012 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB10TAGCTCACTCATTAGGCAC-TCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.A dual-labelled sandwich detection system was developed using biotinylated RAB10 and FITC-labelled RAB35, with a limit of detection (LOD) of 10^2 CFU/mL of S. aureus, and showed 2.7-fold more signal than other bacteria.10Flow Cytometry46 ± 24 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1013 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB20TAGCTCACTCATTAGGCAC-GCGTTACGTTAGTGGCCGCCTATGAGGACAGGCGGTTGTA-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry128 ± 45 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1014 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB28TAGCTCACTCATTAGGCAC-TGGACGTCGTGGCGGAGGTTTTATAAAACGGCGCCACTGT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry49 ± 39 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1015 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB35TAGCTCACTCATTAGGCAC-GGGGGGTTGTGCCATTTAAGATGACCGGTTGCCGCGATTT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.A dual-labelled sandwich detection system was developed using biotinylated RAB10 and FITC-labelled RAB35, with a limit of detection (LOD) of 10^2 CFU/mL of S. aureus, and showed 2.7-fold more signal than other bacteria.10Flow Cytometry34 ± 5 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1016 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB1TAGCTCACTCATTAGGCAC-CGGGTGGGCTCCAATATGAATCGCTTGCCCTGACGCTATCT-GCATAGTTAAGCCAGCC775'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry56 ± 87 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1017 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB3TAGCTCACTCATTAGGCAC-CGTAGTCTAGTGTCGATTAGTTTCCTTGAGACCTTGTGCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry37 ± 112 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1018 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB5TAGCTCACTCATTAGGCAC-CGTAGTCTAGTGTCGATTAGTTTCCTTGCTATTGCAGACCTTGTGCT-GCATAGTTAAGCCAGCC835'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry58 ± 14 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1019 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB9TAGCTCACTCATTAGGCAC-TCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1020 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB21TAGCTCACTCATTAGGCAC-GGGGGGTTGTGCCATTTAAGATGACCGGTTGCCAAGATG-GCATAGTTAAGCCAGCC755'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1080 https://doi.org/10.1002/slct.2018010082018Exploiting Stokes and anti-Stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applicationsS. aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTCCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellAptamer-functionalized QD and UCNP nanoprobes conjugated with partially complementary DNA-modified magnetic beads for separation of different bacteria.The limit of detection was 15 cfu/mL for S. aureus with a linear range from 10(2)-10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1084 297296422018Design and fabrication of an electrochemical aptasensor using Au nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite for rapid and sensitive detection of Staphylococcus aureusStaphylococcus aureus aptamerTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC46N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an impedimetric aptasensor using AuNPs/CNPs/CNFs nanocomposites for the rapid detection of S. aureus.Exhibited a wide linear dynamic range 1.2 × 10(1) to 1.2 × 10(8) CFU/mL with a LOD of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1150 295503462018An aptasensor for staphylococcus aureus based on nicking enzyme amplification reaction and rolling circle amplificationAptamerTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA chemiluminescence aptasensor for S. aureus detection based on aptamer recognition and the DNA amplification cycle (NEAR-RCA) was developed.Detect S. aureus specifically with a good linear correlation at 5-10(4) CFU/mL and limit of detection (LoD) of 5 CFU/mL.N/AN/A210.70 ± 135.91 nMBiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1180 322548372018Sensitive and specific detection of clinical bacteria via vancomycin-modified Fe3O4@Au nanoparticles and aptamer-functionalized SERS tagsSa1GCAATGGTACGGTACTTCCACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDevelop a dual-recognition SERS platform using vancomycin-modified Fe3O4@Au magnetic nanoparticles (Fe3O4@Au-Van MNPs) for the detection of E. coli and S. aureus.The LOD of the platform was 20 cells/mL for S. aureus, with a capture efficiency of up to 88.89% within 15 min.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6) and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1183 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC5CCTAACCGATATCACACTCAC-GGCTAGCGGACAGGTGTAGGTCGGAATCCTTCAGCGTAGA-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1184 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC7CCTAACCGATATCACACTCAC-AGTATACCGCTCCACCAGTGTGATATCGGGATCTGCTGAC-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1185 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC10CCTAACCGATATCACACTCAC-GTAGTGAGGTGGGTCGTGAAGGGAGGCACTACTGGTGCGT-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1186 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC13CCTAACCGATATCACACTCAC-GAGGTGAGGGTGCGAGGGTAAGGGGGCAAACCTGGTGCGT-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1187 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC16CCTAACCGATATCACACTCAC-ACGTAGTGAGGGTTGCGAGGGTAAGGTGGTCAGTATACGC-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1200 295338182018Culture-free, highly sensitive, quantitative detection of bacteria from minimally processed samples using fluorescence imaging by smartphoneS. aureus–specific aptamer (Sap)TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (USA300)Surface proteinDeveloped a smartphone-based detection using aptamer-functionalized fluorescent magnetic nanoparticles (FMNPs) for Staphylococcus aureus.Showed a minimum detectable concentration as low as 10 cfu/ml in a peanut milk sample within 10 min.N/AN/A3.03 nMBiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1231 314724132019Vertical capacitance aptasensors for real-time monitoring of bacterial growth and antibiotic susceptibility in bloodS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellVertical-type aptamer-functionalized sensor (aptasensor) for monitoring bacterial growth and antibiotic susceptibility in blood in real-time.Detect bacteria growth in blood at 10(0)-10(3) CFU/mL in real-time within 12 hours and the MIC for P. aeruginosa was approximately 1 μg/mL for both gentamicin and amikacin.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1257 313077212019Dual-recognition surface-enhanced Raman scattering(SERS)biosensor for pathogenic bacteria detection by using vancomycin-SERS tags and aptamer-Fe3O4@AuS. aureus-aptamerGCAATGGTACGGTACTTCCACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a dual-recognition SERS biosensor using Vancomycin-Au@MBA and aptamer-Fe3O4@Au for the detection of pathogenic bacteria.A detection limit of 3 cells/mL with a wide dynamic linear range from 10 to 10(7) cells/mL can be achieved within 50 min.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1278 313808722019A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamersSA31TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA45N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDevelop a transcription aptasensor by using a light-up RNA aptamer for culture-free detection of intact foodborne pathogens.The dynamic range was 10(2)-10(6) CFU/mL, and the LOD of the transcription aptasensor was estimated to be 77.0 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1279 316173472019Ultrasensitive Fluorometric Angling Determination of Staphylococcus aureus in Vitro and Fluorescence Imaging in Vivo Using Carbon Dots with Full-Color EmissionS. aureus aptamer (Apt)GCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellAn ultrasensitive magnetic fluorescence aptasensor was designed using Fe3O4/CD for the separation and detection of S. aureus.The FRET-based aptasensor achieved a wide linear range of 50–10(7) CFU/mL and a detection limit of 8 CFU/mL.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1332 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens10A-S1AAAAAAAAAA-TTTTT-TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC77N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.The detection limit of the current SPR aptasensors is 1 x 10(6) CFU/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-PolyA (10A) and Five thymine (5T)N/AN/ABest CandidateN/AN/A
ABdb_1333 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens10A-S2AAAAAAAAAA-TTTTT-GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA103N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (10A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1336 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens30A-S1AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-TTTTT-TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC97N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (30A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1337 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens30A-S2AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-TTTTT-GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA123N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (30A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1338 306220362019An electrochemical aptasensor for staphylococcal enterotoxin B detection based on reduced graphene oxide and gold nano-urchinsAptamer SEBTGCAGGATCCGGTATCCGTGCACACACACCCAACAACCAGCTGCCGCACCGGAGGAATTCTCGT64N/AssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)An electrochemical aptasensor was developed using a screen-printed electrode modified with reduced graphene oxide (rGO) and gold nano-urchins (AuNUs) for the detection of SEB.A wide linear range of 5.0–500.0 fM was observed, with a detection limit of 0.21 fM.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1347 311964182019Functional chimera aptamer and molecular beacon based fluorescent detection of Staphylococcus aureus with strand displacement-target recycling amplificationAptamerCACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT48N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a fluorescent detection of S. aureus based on a functional chimaera and a molecular beacon.Display a broad concentration range from 80 CFU/mL to 8 × 10(6) CFU/mL and the detection limit of 39 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1350 323331132020Inhibitory effects of aptamer targeted teicoplanin encapsulated PLGA nanoparticles for Staphylococcus aureus strainsSA20GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus) susceptible strains and MRSAWhole cellAptamer-PLGA nanoparticles (Apt-teicoplanin-PLGA NPs) for the delivery of teicoplanin antibiotic.MICs of teicoplanin decreased by 32- and 64-fold for susceptible strains and MRSA strains, respectively.N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1373 327925732020Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolatesAptamer 5GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (P101, T82, C970, R4308, R4319)Whole cellElectrical AST (e-AST) chips composed of 60 aptamer-functionalized capacitance sensors to detect bacteria and measure the antibiotic susceptibility to 11 antibiotics at five different concentrations.The discrepancies between e-AST and BMD tests were estimated to be 2.20% mE, 0.38% ME, and 0.38%, which are lower than the FDA requirements (mE ≤ 10%, ME ≤ 3%, and VME ≤ 1.5%).N/AN/AN/ADiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_1410 https://doi.org/10.1007/s12161-020-01821-42020Fluorescent Turn-on Aptasensor of Staphylococcus aureus Based on the FRET Between Green Carbon Quantum Dot and Gold NanoparticleStaphylococcus aureus aptamerGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped FRET-based aptasensor with CQDs and GNPs for the detection of S. aureus.Linear detection range of 10⁸ to 10¹ CFU/mL with a detection limit (LOD) of 10 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1436 323506132020A fluorescent aptasensor for Staphylococcus aureus based on strand displacement amplification and self-assembled DNA hexagonal structureAptamerCACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT48N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA fluorescent aptasensor based on MB-apt-cDNA duplex for S. aureus in milk samples.Exhibits a broad linear range from 7 to 7 × 10(7) CFU/mL, with a detection limit of 1.7 CFU/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1488 323475302020A sensitive and rapid bacterial antibiotic susceptibility test method by surface enhanced Raman spectroscopyS. aureus aptamerTCCCTACGGCGCTAACCTCCCAACCGCTCCACCCTGCCTCCGCCTCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (CICC 21600)Whole cellDeveloped a rapid antibiotic susceptibility test (AST) method and determined the MIC value by the Bacteria-aptamer@AgNPs-SERS method.When treated with 2(−1) μg/mL vancomycin for 1 h, the Raman peak intensity of S. aureus was 556 a.u., and when the concentration of antibiotics was sub-MIC (2(−2) and 2(−3) μg/mL), the peak value of 735 cm−1 increased, and the Raman intensity was 2177 a.u. and 2903 a.u.N/AN/AN/ADetectionN/AN/AN/AN/AN/AN/AN/A
ABdb_1503 343474272021Aptamer-Functionalized DNA-Silver Nanocluster Nanofilm for Visual Detection and Elimination of BacteriaApt-GTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAACGGGTGGGGTGGGGTGGGG80N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDNA-templated silver nanoclusters (N4-AgNC/Apt-G) for the visual detection and effective elimination of bacteria.After treatment with 5 μM N4-AgNCs/Apt-G, a significantly reduced number of colonies was observed. At the lowest inhibitory concentration of 6.25 μM, the number of colonies decreased by only 4-fold, which is significantly less than the 7-fold reduction observed at 5 μM. Obtained recovery rates ranging from 88% to 115% of S. aureus.N/AN/AN/ADiagnostic/TherapeuticsN/AN/AN4-AgNCs/Apt-G was found to be nontoxic at low concentrations, and cell viability was more than 80% at a concentration of 6.25 μM.N/AN/AN/AN/A
ABdb_1544 340516012021Sensitive detection of S. Aureus using aptamer- and vancomycin -copper nanoclusters as dual recognition strategyAptamerGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped an aptamer- and antibiotic-based dual detection sensor, combines copper nanoclusters (CuNCs) for the detection of S. aureus.Showed a linear range of 10(2)-10(8) CFU/mL, and a detection limit of 80 CFU/mL after 45 min.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1545 https://doi.org/10.1016/j.electacta.2021.1396332021A new electrochemical aptasensor based on gold/nitrogen-doped carbon nano-onions for the detection of Staphylococcus aureusAnti-S. aureus aptamerTCGGCACGTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC43N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an electrochemical aptasensor based on SPEs modified with AuNPs and NCNOs for the detection of S. aureus.Measured S. aureus quickly with a limit of detection of 3 CFU/mL in the linear range of 10–10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1586 340744322021One-step colorimetric detection of Staphylococcus aureus based on target-induced shielding against the peroxidase mimicking activity of aptamer-functionalized gold-coated iron oxide nanocompositesAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped a colorimetric sensor using apt-Fe3O4/Au nanocomposites for the detection of Staphylococcus aureus.Display a linear range from 10 to 10(6) CFU/mL, with the visible limit of detection as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1588 347535772021An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tagsS. aureus-aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an aptasensor using WGA modified-Fe3O4@Au MNPs and Streptavidin (SA)/aptamer co-functionalized SERS tags for S. aureus and L. mono detection.The LOD for S. aureus was 3 cells/mL and a favorable linear relation (10-10(7) cells/mL).N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_1594 340415802021Fabrication of gold/silver nanodimer SERS probes for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an aptamer-facilitated gold/silver nanodimer SERS probe for the simultaneous detection of the two bacteria with the help of magnetic separation enrichment.Achieved a linear range of 3.2 × 10(2) to 3.2 × 10(7) cfu/mL, with a detection limit of 96 cfu/mL.N/AN/AN/ABiosensorN/A5′-SH-A-Rox and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1596 344067472021Upconversion Nanoprobes Based on a Horseradish Peroxidase-Regulated Dual-Mode Strategy for the Ultrasensitive Detection of Staphylococcus aureus in MeatS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a colorimetric and fluorescent dual-mode nanoprobe using apt-MNPs and HRP-UCNPs-cDNA for the detection of S. aureus.Obtained limits of detection of 22 CFU/mL for fluorescence and 20 CFU/mL for colorimetry in a linear range of 56–5.6 × 10(6) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1611 355737942022Aptamer-Targeted Drug Delivery for Staphylococcus aureus BiofilmSA31GCAATGGTACGGTACTTCC-TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (DSM 20231)Whole cellAptamer-targeted liposomes encapsulating antibiotics for accumulation and delivery to eradicate S. aureus biofilm.SA31-modified liposomes fully eradicated all viable, culturable bacteria in all biofilm samples.N/AN/AN/ATargeted Delivery/TherapeuticsN/A3'-5-propargylamino-ddUTP-Cy5 or 3'-N3 modifiedN/ASA23 appeared more stable than the other aptamers in plasma over a 16-hour incubation period.N/AN/AN/A
ABdb_1639 https:doi.org10.1016j.arabjc.2022.1042742022Application of G-quadruplex aptamer conjugated MSNs to deliver ampicillin for suppressing S. aureus biofilm on mice bonePA763’-ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCTACAATCGTAATCAGTTAG-5’76N/AssDNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)MSNs-APT-AMP nanosystem for antibiofilm activity against S. aureus biofilm.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1640 https:doi.org10.1016j.arabjc.2022.1042742022Application of G-quadruplex aptamer conjugated MSNs to deliver ampicillin for suppressing S. aureus biofilm on mice bonePA633’-ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCTACAAT-5’63N/AssDNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)MSNs-APT-AMP nanosystem for antibiofilm activity against S. aureus biofilm.No significant biofilm on the surface of the bone after 48 h treatment with 100 µg/mL of the three-component system.N/AN/AN/ATherapeuticsN/AN/ANo significant toxicity at 100 µg/mL for MCF-7 cells in 48 h.N/ABest CandidateN/AN/A
ABdb_1641 https:doi.org10.1016j.snb.2022.1327522022Novel nanozyme-catalyzed and magnetically assisted colorimetric biosensor for Staphylococcus aureus detection with a low matrix effect from complex environmentsSA31GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Staphylococcus aureus Protein A (SpA)Vancomycin-modified magnetic nanoparticles (Fe3O4 @Au-Van MNPs) combined with an aptamer were prepared for S. aureus detection.The biosensor can distinguish more than 100 CFU/mL of pathogens by the naked eye and LOD of 2 CFU/mL for S. aureus.N/AN/AN/ABiosensorN/AFAM LabeledN/AN/AN/AN/AN/A
ABdb_1650 358691072022An innovative dual recognition aptasensor for specific detection of Staphylococcus aureus based on Au/Fe3O4 binary hybridAptGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 6538)Whole cellDeveloped an imprinted aptasensor (apt-AuNPs@ Fe3O4) for the quantification of S. aureus.Exhibited a wide linear range of 10(1)-10(7) CFU/mL with a Limit of Detection (LOD ) of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AThe aptasensor retained approximately 95% of its original activity for four weeks.N/AN/AN/A
ABdb_1651 358297782022An ultrasensitive sandwich-type electrochemical aptasensor using silver nanoparticle/titanium carbide nanocomposites for the determination of Staphylococcus aureus in milkAptsGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CICC 23656)Whole cellDeveloped a novel sandwich-type electrochemical aptasensor using AgNPs@Ti3C2 nanocomposites for the detection of S. aureus.Showed a low detection limit (LOD) of 1 CFU/mL and a linear range of 52–5.2 × 10(7) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/A92.68% of the initial current of the aptasensor was reserved when stored for 15 days at 4°C.N/AN/AN/A
ABdb_1652 358702832022An ultrasensitive and dual-recognition SERS biosensor based on Fe3O4@Au-Teicoplanin and aptamer functionalized Au@Ag nanoparticles for detection of Staphylococcus aureusS. aureus AptGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellThe SERS biosensor based on Fe3O4@Au-Tcp NPs as a capture probe and Au@Ag-DTNB-Apt NPs as a signal probe for the detection of S. aureus.Showed detection limit of 1.09 CFU/mL with a broad dynamic linear range of 7.6 × 10(1)-7.6 × 10(7) CFU/mL within 50 min.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1658 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K3CCGGAATTCCTAATACGACTCCCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTGTATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellDetermine the antibiofilm activity and binding specificity of the polyclonal DNA aptamers on S. aureus BPA-12 and E. coli EPEC 4.Showed the percentage of antibiofilm activity against S. aureus BPA-12 (25.8%) and E. coli EPEC 4 (0.3%).N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1659 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K4CCGGAATTCCTAATACGACTCCCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCCTATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the percentage of antibiofilm activity against S. aureus BPA-12 (26.3%) and E. coli EPEC 4 (2.8%).N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1660 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K6CCGGAATTCCTAATACGACTCGCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGATATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the highest percentage of antibiofilm activity against S. aureus BPA-12 (37.4%).N/AN/AN/ATherapeuticsN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_1661 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K13CCGGAATTCCTAATACGACTCCACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGGTATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the percentage of antibiofilm activity against S. aureus BPA-12 (31.8%) and E. coli EPEC 4 (9.4%).N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1662 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K15CCGGAATTCCTAATACGACTCCAGGACAGTACTCTGGACGGCAATACGTATATACGTACGGTATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the percentage of antibiofilm activity against S. aureus BPA-12 (19.1%) and E. coli EPEC 4 (-1.3%).N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1663 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K20CCGGAATTCCTAATACGACTCCCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGATATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the highest percentage of antibiofilm activity against E. coli EPEC 4 (15.4%).N/AN/AN/ATherapeuticsN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_1679 350312532022Architecting of an aptasensor for the staphylococcus aureus analysis by modification of the screen-printed carbon electrode with aptamer/Ag-Cs-Gr QDs/NTiO2AptamerTCGGCACGTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC43N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAn electrochemical aptasensor employing Apt/Ag–Cs-Gr QDs/NTiO2/SPCE was developed for the detection of S. aureus.Limit of Detection (LOD) of 3.3 CFU/mL and dynamic range of 10–5 × 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/ACompared with the initial current response, the aptsensor response changed less than 10% after 100 cycles’ continuous CV scans.N/AN/AN/A
ABdb_1680 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K3CCGGAATTCCTAATACGACTC-CCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)36,70 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1681 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K4CCGGAATTCCTAATACGACTC-CCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1682 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K6CCGGAATTCCTAATACGACTC-GCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K6 has the highest binding affinity to S. aureus BPA-12.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)17,01 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1683 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K13CCGGAATTCCTAATACGACTC-CACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)60,69 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1684 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K15CCGGAATTCCTAATACGACTC-CAGGACAGTACTCTGGACGGCAATACGTATATACGTACGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20,90 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1685 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K20CCGGAATTCCTAATACGACTC-CCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1718 360589392022Multiple amplification-based fluorometric aptasensor for highly sensitive detection of Staphylococcus aureusAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped a fluorometric aptasensor for the detection of S. aureus using magnetic nanoparticles (MNPs).The proposed fluorometric aptasensor displays LOD of (1.23 cfu/mL, a wide linear range (1 ~ 10(8) cfu/mL), and a fast detection speed (~ 1.5 h).N/AN/AN/ABiosensorN/A5'-Biotinylated (Biotin-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1725 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA37 (SA-10R-37)GCTTCCAGCTTATTGAAT-AAAGACGGGGGGGGGGACCGGCGTATGAGTGAAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.Achieved a limit of detection (LOD) of 39 CFUs and 414 CFUs in buffer and spiked tap water samples, respectively, with the cognate pair of SA37@Cy5 and SA81@FAM.10Flow Cytometry16.5 ± 3.41 nMBiosensorWhole Cell-SELEX5'-Cyanine5 (Cy5) LabeledN/AN/ABest CandidateN/AN/A
ABdb_1726 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA81 (SA-10R-81)GCTTCCAGCTATTGAA-AACGAGGCGCAGGGGGAGGGGGTGGTACAGATAAGATGGGG-GCGCTGAAGCGCGGAAGC755'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.Achieved a limit of detection (LOD) of 39 CFUs and 414 CFUs in buffer and spiked tap water samples, respectively, with the cognate pair of SA37@Cy5 and SA81@FAM.10Flow Cytometry14.47 ± 8.18 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1727 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA25 (SA-9R-25)GCTTCCAGCTTATTGAAT-GGGGAAGGTCGTCCGACGAACCCGGTCAGATAGGGTGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry44.92 ± 1.36 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1728 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA28 (SA-10R-28)GCTTCCAGCTTATTGAAT-GCGGCCACGGAGGGGGTGCCGGGCGTGGAATAAGATGTGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry77 ± 1.22 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1729 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA35 (SA-10R-35)GCTTCCAGCTTATTGAAT-CACAGGTGTGGGGAGGTCCCCATGGAGGTGGTTCAATG-GCGCTGAAGCGCGGAAGC745'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry58.77 ± 0.73 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1730 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA40 (SA-10R-40)GCTTCCAGCTTATTGAAT-CGACGTGAAGCAATCATGGGTGGGGTACGTCGGGTCATGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry126.95 ± 0.51 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1731 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-9R-12GCTTCCAGCTTTATGAAT-TGGGCGTGGCGACGGTGTCAGGTCGGGGCGGTAAGACGAGG-GCGCTGAAGCGCGGAAGC775'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1732 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-9R-57GCTTCCAGCTTATTGAAT-GGAACGAGATGGGGCATGGCGCCACGGAGGGGAATCAAGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1733 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-1GCTTCCAGCTTATTGAAT-CGAGGGTAAGGGAAGTATGGCCGGTGCCCTGGAGTCAATG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1734 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-18GCTTCCAGCTTATTGAAT-CGGGTGGGGGGAGCGACAGACGGAAAAGCCTGGGTCAATAG-GCGCTGAAGCGCGGAAGC775'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1735 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-55GCTTCCAGCTTATTGAAT-GGAGGGGCAGGGCATGGAGCTCGACATTCATGAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1736 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-59GCTTCCAGCTTATGGAAT-GTGCCGAGGCGATACATGCACAGGCATGGTGGGATAAGGTCGGG-GCGCTGAAGCGCGGAAGC805'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1737 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-69GCTTCCAGCTTATTGAAT-CGGAGAGTCTGGCGCAGCCCTACGCCGATGTAAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1738 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-72GCTTCCAGCTTATTGAAT-CGAGGTGTGGGAGACAACGCTCCGTGACCGAGGGGAAATG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1740 361089852022Naked-eye detection of Staphylococcus aureus in powdered milk and infant formula using gold nanoparticlesAnti-S. aureus aptamer (Apt1)TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (FPR3757 USA300)Whole cellDeveloped a colorimetric LSPR aptasensor using gold nanoparticles for the detection of S. aureus in milk and infant formula.Could be visually detected within 30 min, with detection limits of 7.5 × 10(4) CFU/mL and 8.4 × 10(4) CFU/mL in milk and infant formula, respectively.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1746 https://doi.org/10.1016/j.snb.2021.1308792022Aptamer-conjugated magnetic Fe3O4@Au core-shell multifunctional nanoprobe: A three-in-one aptasensor for selective capture, sensitive SERS detection and efficient near-infrared light triggered photothermal therapy of Staphylococcus aureusAptGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CMCC 26003)Whole cellDeveloped an aptasensor based on Fe3O4@Au nanocomposites (NCs) for capture, SERS detection, and photothermal therapy (PTT) of S. aureus.The detection limit is 25 cfu/mL, the cell capture efficiency (CCE) is as high as 68% and it has a high photothermal conversion efficiency of 39.28%.N/AN/AN/ADiagnostic/TherapeuticsN/A5'-ThiolatedThe cytotoxicity of Fe3O4@Au-Apt NCs to S. aureus is negligible.The SERS intensity of S. aureus on the Fe3O4@Au-Apt NCs remains almost unchanged within 12 days.N/AN/AN/A
ABdb_1768 https:doi.org10.1016j.snb.2023.1342182023Dual-mode sensing platform based on aptamer-tunable catalytic activity of mesoporous polydopamine/MnO2 nanozymes for detecting S. aureusSA31GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTA87N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellColourimetric-electrochemical sensing platform to detect S. aureus based on a dual-modal strategy.Shows a low detection limit in 3 CFU/mL with a linear range between 5 and 10(7) CFU/mL and in a real sample with the recovery of 95.15%− 115.28%.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1776 369069922023An ultrasensitive electrochemical aptasensor using Tyramide-assisted enzyme multiplication for the detection of Staphylococcus aureusSA37GCTTCCAGCTTATTGAATAAAGACGGGGGGGGGGACCGGCGTATGAGTGAAGATGGGGGCGCTGAAGCGCGGAAGC76N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellAn electrochemical aptasensor based on the tyramide signal amplification (TSA) technology was developed for the detection of S. aureus.Achieved a limit of detection (LOD) of 3 CFU/mL in buffer and 8 CFU/mL in both tap water and beef broth.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1777 368320382023Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu2WO4(OH)2 and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus DetectionAptTCGGCACGTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC43N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an aptasensing Platform based on a Green Nanocomposite consisting of Spongin Decorated Cu2WO4(OH)2 and AgNPs for Staphylococcus aureus detection.Measured S. aureus under a linear concentration range of 10–10(8) CFU/mL and a limit of quantification and detection of 12 and 1 CFU/mL, respectively.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1778 373272072023FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected AptamersT1-280ACTGTCrGrCrGrCrArCrGrCrGrUrGrUrGrUrArGrUrArCrArCrArCrGrArUrCrGrCrGrCrGrCrArCrArArUrArU46N/AssRNAStaphylococcus aureus (S. aureus)Iron-regulated Surface Determinant Protein A (IsdA)Develop a FRET-based aptasensor for single-molecule detection of IsdA.LOD value of T1-280 RNA was 113 pM.N/AFluorescence Resonance Energy Transfer Assay (FRET)2.2 ± 0.5 nMDetectionIn-silico Method5'-Cyanine3 (Cy3) Labeled or 5'-Biotinylated and 5′ end by six nucleotides (ACT GTC), rX = RNA nucleotideN/AN/AN/AN/AN/A
ABdb_1779 373272072023FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected AptamersT2-139040ACTGTCrArArUrUrUrGrArArUrArUrArUrUrArGrUrGrCrGrCrGrCrCrGrUrArGrUrGrUrGrUrArArArArArUrU46N/AssRNAStaphylococcus aureus (S. aureus)Iron-regulated Surface Determinant Protein A (IsdA)Develop a FRET-based aptasensor for single-molecule detection of IsdA.LOD value of T2-139040 RNA was 17 pM.N/AFluorescence Resonance Energy Transfer Assay (FRET)1.0 ± 0.3 nMDetectionIn-silico Method5'-Cyanine3 (Cy3) Labeled or 5'-Biotinylated and 5′ end by six nucleotides (ACT GTC), rX = RNA nucleotideN/AN/AN/AN/AN/A
ABdb_1780 373272072023FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected AptamersT3-117558ACTGTCrArArUrUrUrGrArGrUrGrUrGrUrGrArUrCrArUrArUrArUrCrGrUrArGrCrGrCrGrCrUrArCrArArCrC46N/AssRNAStaphylococcus aureus (S. aureus)Iron-regulated Surface Determinant Protein A (IsdA)Develop a FRET-based aptasensor for single-molecule detection of IsdA.LOD value of T3-117558 RNA was 11 pM.N/AFluorescence Resonance Energy Transfer Assay (FRET)0.7 ± 0.4 nMDetectionIn-silico Method5'-Cyanine3 (Cy3) Labeled or 5'-Biotinylated and 5′ end by six nucleotides (ACT GTC), rX = RNA nucleotideN/AN/AN/AN/AN/A
ABdb_1781 373272072023FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected AptamersA14ACTGTCCACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT54N/AssDNAStaphylococcus aureus (S. aureus)Iron-regulated Surface Determinant Protein A (IsdA)Develop a FRET-based aptasensor for single-molecule detection of IsdA.LOD value of A14 DNA was 485 pM.N/AFluorescence Resonance Energy Transfer Assay (FRET)4 ± 2 nMDetectionN/A5'-Cyanine3 (Cy3) Labeled or 5'-Biotinylated and 5′ end by six nucleotides (ACT GTC)N/AN/AN/AN/AN/A
ABdb_1783 365492132023Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tagsApt2GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CMCC 26003)Whole cellA SERS platform using aptamers modified-Fe3O4@SiO2-Au NCs was developed for simultaneous detection of E. coli and S. aureus.The detection limit for S. aureus was as low as 10 cfu/mL, and the linear concentration ranged from 10(1)-10(8) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) or 5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1784 https://doi.org/10.1016/j.snb.2023.1335542023A novel Aptamer-induced CHA amplification strategy for ultrasensitive detection of Staphylococcus aureus and NIR-triggered photothermal bactericidal Activity based on aptamer-modified magnetic Fe3O4@AuNRsAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a Fe3O4 @AuNRs-aptasensor based on CHA for ultrasensitive detection of S. aureus.Achieved a linear response ranging from 10(2) to 10(6) CFU/mL and a detection limit of 10(1) CFU/mL under optimal conditions.N/AN/AN/ABiosensorN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1799 369619212023Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell LevelS. aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellAn electrochemical sandwich sensor was developed for the detection of a single bacterial cell based on dual recognition by the bacteria-imprinted polymer film (BIF) and aptamer (Au@Fc-Apt).The sensor could detect as low as 10 CFU/mL in a milk sample and 1 CFU/mL in PBS with a linear range from 10 to 10(5) CFU/mL for S. aureus.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AAfter storage at 4°C for 17 days, the sensor still retained about 92% of the initial detection signal.N/AN/AN/A
ABdb_1800 https://doi.org/10.1002/celc.2023002572023Rolling Circle Amplification/G-Quadruplex-Based Dual-Signal Ratiometric Electrochemical Aptasensor for Ultrasensitive Detection of Pathogenic BacteriaAptamer (P1)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA87N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923 and ATCC 29213)Whole cellDeveloped a ratiometric dual-signal electrochemical biosensor for ultrasensitive detection of S. aureus based on an aptamer recognition-induced rolling circle amplification (RCA)/G-quadruplex strategy.Showed a good linear relationship between 10–10(6) CFU/mL with a detection limit of 10 CFU/mL.N/AN/AN/ABiosensorN/AFerrocene (Fc) conjugatedN/AN/AN/AN/AN/A
ABdb_1815 https://doi.org/10.1016/j.cej.2023.1430992023Design nanoprobe based on DNA tetrahedron supported hybridization chain reaction and its application to in situ analysis of bacteriaAptamer (AA)AGTGCGCAGCTAGCAAGGCCTTTTTTTTTTGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA118N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 6538)Whole cellDeveloped a nanoprobe (DTAAT) based on DNA tetrahedral supported hybridization chain reaction (HCR) for in situ analysis of SA.Detect SA with the detection limit as low as 26 CFU/mL and linear range of 50-5×10(6) CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM Labeled and 3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1817 367057342023A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS techniqueS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CICC 10473)Whole cellDeveloped an assembled aptasensor based on Fe3O4@Au@Ag nanocomposites for the detection of various pathogenic bacteria.Showed a linear range of 10-10(7) CFU/mL, with a detection limit of 5.70 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1824 https://doi.org/10.1016/j.microc.2023.1086052023A SERS biosensor based on aptamer-based Fe3O4@SiO2@Ag magnetic recognition and embedded SERS probes for ultrasensitive simultaneous detection of Staphylococcus aureus and Escherichia coliS. aureus aptamer (P1)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (BNCC 186335)Whole cellDeveloped a SERS biosensor based on aptamer-modified Fe3O4@SiO2@Ag and Au-MPBA/DTNB@Ag for simultaneous detection of S.aureus and E.coli.Rapidly detect S. aureus with the limit of detection (LOD) of 1 CFU/mL in 20 min and a linear range from 10(1) ∼ 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1826 https://doi.org/10.1016/j.microc.2023.1086812023An electrochemical and colorimetric dual-mode aptasensor for Staphylococcus aureus based on a multifunctional MOF and magnetic separation techniqueS. aureus AptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped a colorimetric and electrochemical dual-mode aptasensor based on ssDNA-Au/CuMOF and MB-Apt for S. aureus detection.Display a broad linear range from 10 to 10(8) CFU/mL, with colorimetric minimum resolution of 48 CFU/mL, and electrochemical detection limit of 5 CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1830 382503552024Rapid Detection and Identification of Vancomycin-Sensitive Bacteria Using an Electrochemical Apta-SensorApt1TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (RN4220)Whole cellA portable electrochemical biosensor was developed for the identification of Gram-positive bacteria based on a vancomycin-modified screen-printed carbon electrode.Demonstrated that capture was achieved in 10 min, with a limit of detection of only 2.7 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1840 387706562024Sensitive and on-Site Detection of Staphylococcus aureus Based on CRISPR/Cas 13a-Assisted Chemiluminescence Resonance Energy TransferS. aureus AptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDevelop a CRISPR/Cas 13a-assisted CRET-based strategy for the detection of S. aureus in real samples.Successfully detected S. aureus in drinking water and milk with detection limits of 20 and 30 cfu/mL, respectively, within the recovery of 90.07–105.50%.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1841 397279012024Competitive Electrochemical Apta-Assay Based on cDNA-Ferrocene and MXenes for Staphylococcus aureus Surface Protein A DetectionAPTATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Staphylococcus aureus Protein A (SpA)Developed a competitive aptasensor based on a ferrocene (Fc)-labeled cDNA hybridized (cDNA-Fc S13) on a specific aptamer (APT) for PrA in the presence of MXene nanosheets for the indirect detection of S. aureus.Displayed a linear range of 10–125 nM, an LOD of 3.33 nM, and a response time under 40 min.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1846 381692792024"Five birds one stone" tri-modal monitoring driven lab-on-magnetic aptasensor for accurate pathogen detection and enhanced germicidal applicationAnti-S. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 65389)Whole cellA fluorescence-colorimetric-photothermal tri-modal sensing platform was developed for the detection of S. aureus by apt/KCl@CDs and magnetic multi-walled carbon nanotube composites (M-MWCNTs).The detection limits of fluorescence, colorimetric and photothermal models were 4.81 cfu/mL, 3.40 cfu/mL and 6.74 cfu/mL, respectively with the same linear range of 10 - 1.0 ×10(7) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1849 https://doi.org/10.1016/j.biosx.2023.1004362024DNA origami-enhanced binding of aptamers to Staphylococcus aureus cellsPA#2/8 [1–58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) SA5Staphylococcus aureus Protein A (SpA)Developed a DNA origami-based biosensor for the detection of SA.Five-times higher affinity of the aptamer-functionalized DNA origami compared to pure aptamers.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)160 ± 9 nMBiosensorN/A5'-PolyA (21A) or PolyT (21 T)N/AN/AN/AN/AN/A
ABdb_1851 381804962024Development of an electrochemical sensitive aptasensor based on a zeolite imidazolate framework-8 and gold nanoparticles for the determination of Staphylococcus aureus bacteriaAptamerTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC46N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an electrochemical aptasensor based on a glassy carbon electrode (GCE) modified with zeolite imidazolate framework-8 (ZIF 8) and gold nanoparticles (AuNPs) for the detection of S. aureus.Showed a linear response in the concentration range of 1.5 × 10(1) to 1.5 × 10(7) CFU mL−1 and a detection limit of 3.4 CFU/mL.N/AN/A(9.04 ± 1.27) × 10(−1) nMBiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AResponse of the proposed aptasensor reached 98.9%, 94.3%, and 92.8% of the initial response on the first, second, and third days, respectively, when kept at 4°C.N/AN/AN/A
ABdb_1852 377516322024A novel paper-based electrochemiluminescence biosensor for non-destructive detection of pathogenic bacteria in real samplesAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTGCTAACCCCCCTTAATCCCCCC104N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a paper-based electrochemiluminescence (ECL) aptasensor based on a Ru-MOF-5 NFs modified Indium tin oxide (ITO) electrode for the detection of S. aureus.Exhibited a linear range from 5 to 10(8) CFU/mL, and a limit of detection (LOD) of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AAfter 9 days at 4°C, the ECL signal of aptsensor retained 95.8% of its initial value, suggesting satisfactory storage stability.N/AN/AN/A
ABdb_1869 376398932024Nano-biosensor based on manganese dioxide nanosheets and carbon dots for dual-mode determination of Staphylococcus aureusAptamer1TCGGCACGTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC43N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a fluorescence and colorimetry dual-mode sensor to detect S. aureus by MnO2 NSs and CDs.Had a broad linear range of 37 ∼ 3.7 × 10(6) CFU/mL and low detection limits of 9 CFU/mL (ratiometric fluorescence) and 22 CFU/mL (colorimetry).N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1870 376398932024Nano-biosensor based on manganese dioxide nanosheets and carbon dots for dual-mode determination of Staphylococcus aureusAptamer2GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a fluorescence and colorimetry dual-mode sensor to detect S. aureus by MnO2 NSs and CDs.Had a broad linear range of 37 ∼ 3.7 × 10(6) CFU/mL and low detection limits of 9 CFU/mL (ratiometric fluorescence) and 22 CFU/mL (colorimetry).N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1872 386698462024Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensorsS. aureus aptamerAACGAGGCGCAGGGGGAGGGGGTGGTACAGATAAGATGGGG41N/AssDNAStaphylococcus aureus (S. aureus) (CMCC(B) 26003)Whole cellDeveloped an aptamer-functionalized CNT-FET biosensor for the detection of these foodborne pathogens.Could detect S. aureus at a limit of detection (LOD) as low as 1.2 CFU in PBS buffer with a linear range from 10(2)-1.28 × 10(4) CFU/mL.N/AFlow Cytometry0.65 ± 0.2 μMBiosensorN/A5'-Thiolated (HS-(CH2)6), 5'-FAM Labeled and 3'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1889 398941032025Introduce a novel, extremely sensitive aptamer against staphylococcal enterotoxin type DAptamer 1CCTAACCGATATCACACTCAC-GGGTGCAGCCCGGAGCGGCTTGCATGATTGGGTTGGTGGG-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-N40-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin D (SED)Isolate high-affinity ssDNA aptamers with specificity for SED.The limit of detection (LOD) for SED was determined to be 45 nM.10Surface Plasmon Resonance (SPR) and Enzyme-Linked Apta-Sorbent Assay (ELASA)4.4 ± 2.26 nM (by SPR) and 13.43 nM (by ELASA)DiagnosticWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1895 397933722025Aptamer-molecularly imprinted polymer sensors for the detection of bacteria in waterS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) strain SH1000Whole cellDeveloped an electrochemical sensor based on aptamer-molecularly imprinted polymer (Apta-MIP) for the multiplexed detection of Staphylococcus aureus and Escherichia coli.Can detect S. aureus with a limit of detection of 4 CFU/mL and exhibited a broad dynamic range from 1 CFU/mL to 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1905 411236782025Portable AI-driven electrochemical aptasensor for real-time Staphylococcus aureus detection in food and beveragesIsdA-specific aptamerGCGCACGCGUGUGUAGUACACACGAUCGCGCGCACAAUAU40N/AssRNAStaphylococcus aureus (S. aureus) (ATCC 25923)Iron-regulated Surface Determinant Protein A (IsdA)Developed a portable electrochemical aptasensor integrated with machine learning using SPCE/AuNPs for the detection of S. aureus in food and beverage samples.The lowest detection limit for S. aureus was 1 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1913 4018787020253D DNA walkers integrated with self-reporting MOFs: Pioneering ratiometric electrochemical sensing for Staphylococcus aureusS. aureus aptamer (Apt)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a ratiometric electrochemical aptasensor utilizing a magnetic 3D DNA walking machine and self-assembly of MOF for the sensitive detection of S. aureus.Demonstrates a detection range from 10 to 2 × 10(5) CFU/mL and achieves a low LOD of 2.3 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AAfter a duration of seven days, the ratio value maintained 91.89% of its initial intensity.N/AN/AN/A
ABdb_1914 403357842025A novel strategy for ultrasensitive detection and effective inactivation of Staphylococcus aureus based on Fe3O4-QCS-PEI-Cu-aptamer and ladder-branch HCRAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an integrated strategy based on Fe3O4-quaternary ammonium chitosan-polyetherimide-Cu-aptamer (Fe3O4-QCS-PEI-Cu-aptamer) and ladder-branch hybridization chain reaction (HCR) for the detection and inactivation of S. aureus.The sensor demonstrated a limit of detection (LOD) of 3 CFU/mL for S. aureus and, under near-infrared (NIR) irradiation, achieved an antimicrobial efficiency of 99.946%.N/AN/AN/ADiagnostic/TherapeuticsN/A3'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1930 409166372025Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary ElectrophoresisAP3TCCCTACGGCGCTAACCTCCCAACCGCTCCACCCTGCCTCCGCCTCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 6538)Whole cellDeveloped an aptamer-based capillary sieving electrophoresis (CE)-Laser-induced fluorescence (LIF) detection of three bacteria, Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus.The measured limit of detection (LOD) for S. aureus was 1.67 × 10(7) CFU/mL and a linear range of 3.00-150 × 10(7) CFU/mL.N/AN/AN/ADetectionN/AExtension chain for AP3 (T3), 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1934 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 7.1ACACTAGATCAGTCACAG-GATTAAGGATCGTTGTTGGGTGGTTTCCGTAATTACTATC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1935 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 15.1ACACTAGATCAGTCACAG-GGTTGCTCAGGAGAAGGGAACGTGGGTGGTGGCGCGGACG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1936 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 3.1ACACTAGATCAGTCACAG-ACGGGGAGAGGGTCATCTCTGGTTTCGGGGTGCTAATTCT-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1937 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 8.1ACACTAGATCAGTCACAG-CCCCGACCTGTACACGTACTACCCGATGACATTTGGTGTT-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1938 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 13.1ACACTAGATCAGTCACAG-TGGACGCGGCGGGCCGGATTAAGTTGAGGAGAGGGGGGTG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1939 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 16.1ACACTAGATCAGTCACAG-AGTATATATGGATTCCGCAACGTACGCAGGGGGTGTCGCC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1940 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 14.1ACACTAGATCAGTCACAG-CAGAGAGGGGCGGCGAGGGACAAAGGAGGGTAGGTGTGCC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1941 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 12.1ACACTAGATCAGTCACAG-ATCACAGCGGAGGTTTAACGTGTGGCGGGTGTGGGGACATG-GACTTGACTCAGACATCT775'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1942 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 11.1ACACTAGATCAGTCACAG-GAGCAACAGTCTCGCCTTCGTTACTGCGCGCGGGACGGGG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.CA 11.1 exhibited S. aureus binding in a linear range of 10(3) to 10(8) CFU/mL and a limit of detection (LOD) of 10(3) CFU/mL.11Bead-based Binding Assay263.8 ± 78 nMDetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1943 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 17.1ACACTAGATCAGTCACAG-AAGTGGAATCAAGCATGGGTATTTTAAGCATCGTATCTCG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1944 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 5.1ACACTAGATCAGTCACAG-TGTTTCACGGGGGGGAGGGTAACGGAAGCTAATGGTCTCG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1946 413440102026SERS-based Aptasensor using Au NSs@Ag NPs/magnetic graphene oxide assemblies for simultaneous detection of Escherichia coli and Staphylococcus aureusApt2 & Apt4TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAACTTTTTTTTT71N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SERS aptasensor based on aptamer-magnetic graphene oxide (MGO) and dual-functional Au NSs@Ag NPs probes for simultaneous detection of E. coli and S. aureus.The SERS intensity shows a good linear relationship over a range of 50 to 10(6) CFU/mL, with a detection limit of 20 CFU/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-Thiolated (for apt1 & apt2) and 5'-Amidation (NH₂) (for apt3 & apt4)N/AN/AN/AN/AN/A
ABdb_1953 118086912002Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methodsAnti-staphylococcal enterotoxin B (SEB) aptamersN/AN/A5'-CCGGATCCGTTGATA-N20-GTGGTGTTGGCTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Developed an aptamer-based electrochemiluminescence assay using a dsDNA aptamer for biotoxin detection.Detection limit of less than 10 pg SEB with electrochemiluminescence assay.5N/AN/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated (for ECL) or 5'-Amidation (NH₂-(CH2)6) (for colorimetric assays)N/AN/AN/AN/AN/A
ABdb_1988 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4520–8PCGGCPPCGGGPACCPAPPAPCGGPPPAGCCCAGPCAGAA40N/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Staphylococcus aureus Protein A (SpA)Developed slow off-rate modified aptamer (SOMAmer) reagents against S. aureus cell surface-associated proteins, used to capture and detect S. aureus.Efficient capture of Staph. aureus over a wide range of cell densities from 5 × 10(2) CFU/ml to 5 × 10(9) CFU/ml.8Radiolabel Filter Binding Assay0·22 nMDetectionSELEXP=NapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1989 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4503–73AZCZGGZZCAAAGZGGCGAZZGGGCAZCZGGZZZZZAAGZ40N/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor A (ClfA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.Efficient capture of Staph. aureus over a wide range of cell densities from 5 × 10(2) CFU/ml to 5 × 10(9) CFU/ml.8Radiolabel Filter Binding Assay0·79 nMDetectionSELEXZ=BndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1990 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4531–56N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Staphylococcus aureus Protein A (SpA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.Able to bind whole cells of all Staph. aureus strains tested, with a detection limit of approx. 10(4) cells per well (10(5)-10(6) cells/ml).8Radiolabel Filter Binding Assay0·03 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1991 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4522–5N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor A (ClfA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·35 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1992 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4504–27N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor B (ClfB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay1·35 nMDetectionSELEXBndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1993 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4511–67N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor B (ClfB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay3·90 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1994 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4523–79N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor B (ClfB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·47 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1995 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4726–44N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding Protein A (FnbA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay4·38 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1996 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4745–51N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding Protein A (FnbA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·63 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1997 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4506–13N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding protein B (FnbB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay4·73 nMDetectionSELEXBndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1998 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4516–29N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding protein B (FnbB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·63 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1999 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4527–83N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding protein B (FnbB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·84 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2000 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4727–62N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein A (IsdA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·73 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2001 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4746–3N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein A (IsdA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·16 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2002 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4728–7N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein B (IsdB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·14 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2003 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4747–90N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein B (IsdB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay1·98 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2004 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4507–52N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein C (IsdC)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·15 nMDetectionSELEXBndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2005 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4517–71N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein C (IsdC)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·08 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2006 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4528–22N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein C (IsdC)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·07 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2007 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4731–69N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein H (IsdH)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay1·30 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2008 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4730–3N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)SasDDeveloped slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay2·17 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2012 244725392014DNA aptamers as a novel approach to neutralize Staphylococcus aureus α-toxinAT-27N/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAStaphylococcus aureus (S. aureus)α-toxin (exotoxin)Identify aptamers that bind to and inhibit the cytotoxic activity of a-toxin and the upregulation of inflammatory cytokines TNF-a and IL-17.Increased the viability of α-toxin-treated Jurkat T cells to about 85–90%.10N/AN/ATherapeutics/ImmunmodulatorySELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_2013 244725392014DNA aptamers as a novel approach to neutralize Staphylococcus aureus α-toxinAT-33N/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAStaphylococcus aureus (S. aureus)α-toxin (exotoxin)Identify aptamers that bind to and inhibit the cytotoxic activity of a-toxin and the upregulation of inflammatory cytokines TNF-a and IL-17.AT-33 inhibited α-toxin-induced upregulation of TNF-α and IL-17 in Jurkat T cells. Increased the viability of α-toxin-treated Jurkat T cells to about 85–90%.10N/AN/ATherapeutics/ImmunmodulatorySELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_2014 244725392014DNA aptamers as a novel approach to neutralize Staphylococcus aureus α-toxinAT-36N/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAStaphylococcus aureus (S. aureus)α-toxin (exotoxin)Identify aptamers that bind to and inhibit the cytotoxic activity of a-toxin and the upregulation of inflammatory cytokines TNF-a and IL-17.AT-36 inhibited α-toxin-induced upregulation of TNF-α and IL-17 in Jurkat T cells. Increased the viability of α-toxin-treated Jurkat T cells to about 85–90%.10N/AN/ATherapeutics/ImmunmodulatorySELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_2015 244725392014DNA aptamers as a novel approach to neutralize Staphylococcus aureus α-toxinAT-49N/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAStaphylococcus aureus (S. aureus)α-toxin (exotoxin)Identify aptamers that bind to and inhibit the cytotoxic activity of a-toxin and the upregulation of inflammatory cytokines TNF-a and IL-17.Increased the viability of α-toxin-treated Jurkat T cells to about 85–90%.10N/AN/ATherapeutics/ImmunmodulatorySELEX5'-BiotinylatedN/AN/AN/AN/AN/A