Target Organism : Salmonella Typhi

Total records found: 207

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0015 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.4GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UCACUGUUAUCCGAUAGCAGCGCGGGAUGA-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.2.0 μg of RNA aptamer S-PS8.4 effected ca. 71% inhibition of cell invasion by pil+ S. enterica serovar Typhi A21-6 but only ca. 19% inhibition in the case of the pilS::Kmr mutant.8Nitrocellulose Filter Binding Assay8.56 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0016 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.3GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AUUACCUAGAGCGGGAUAAAGUAUAGGUU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0017 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.2GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-CAUCGAGGAGGCGGGAUAUUCGAUGAGUU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0018 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.5GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-GAGUACGAGCGGGAUAGUAAUCGGGUGAU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0019 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.6GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-GAAGGGGUCCGGCUCGGGUGAGGGUCGGGU-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0020 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.9GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AGCUAGCGGGGGGGCUCGACGGUGGUGGGUU-GGGUCAAUGCGUCAUA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0021 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.7GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UAGCGGGAGCUUGGACCUGGUGGUCGCGGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0022 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.1GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UUGGGAUCAGCUCGGCGCUGGAGGAGGGGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0023 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.8GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AACUGUACAUGGGCGCAACAGGGAGUUAGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0067 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 33TTTGGTCCTTGTCTTATGTCCAGAATGC-TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.In a pull-down assay format, detection limits were 10¹–10² CFU S. Typhimurium/9 mL rinsate, while in a recirculation format, detection limits were 10²–10³ CFU/25 mL rinsate.7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0068 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 23TTTGGTCCTTGTCTTATGTCCAGAATGC-CCGCCTTTACTAAATTGACGAACATAGGAATCAATGAAGC-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.N/A7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0069 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 24TTTGGTCCTTGTCTTATGTCCAGAATGC-GGGAGTCAGAACGCCTGGGCAAGCATAGTACTCGCCGGAA-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.N/A7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0070 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 25TTTGGTCCTTGTCTTATGTCCAGAATGC-TAAAGAGGAATCGACAGGCATCAGAGTCCGTAATGCGGGG-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.N/A7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0071 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 45TTTGGTCCTTGTCTTATGTCCAGAATGC-GAGGAAAGTCTATAGCAGAGGAGATGTGTGAACCGAGTAA-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.N/A7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0072 195691562009Immediate detection of living bacteria at ultralow concentrations using a carbon nanotube based potentiometric aptasensorAptamerGGGAACAGUCCGAGCCUCACUGUUAUCCGAUAGCAGCGCGGGAUGAGGGUCAAUGCGUCAUAGGAUCCCGC71N/AssRNASalmonella Typhi (CECT 409)Type IVB Pili structural proteinsDevelop a potentiometric biosensor using an aptamer-SWCNT for selectively detecting a single CFU of ST in close to real time.Display detection range from 0.2 CFU/mL (1 CFU in 5 mL PBS) to 10(6) CFU/mL within 60sec.N/AN/AN/ABiosensorN/A3'-Amidation ((CH2)5-NH₂)N/AN/AN/AN/AN/A
ABdb_0123 218751072011Identification of environmental reservoirs of nontyphoidal salmonellosis: aptamer-assisted bioconcentration and subsequent detection of salmonella typhimurium by quantitative polymerase chain reactionSal aptamerCTCACCAGGAGATTACAACATGG-TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG-AGCTCAGACCAAAAGTGACCATC865'-CTCACCAGGAGATTACAACATGG-N40-AGCTCAGACCAAAAGTGACCATC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDetection of Salmonella Typhimurium by serovar-specific DNA aptamer-conjugated dynabeads in water samples.Sediments from the selected sampling locations also exhibit Salmonella Typhimurium in the range of 3.37 × 10(4) to 3.08 × 10(9) cfu/100 g.N/AN/AN/ADetectionN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0137 231906952012Aptasensors for rapid detection of Escherichia coli O157:H7 and Salmonella typhimuriumA2CCAAAGGCTACGCGTTAACGTGGTGTTGG29N/AssDNASalmonella Typhimurium (S. Typhimurium) (CMCC 50115)Whole cellAptasensors based on label-free aptamers and gold nanoparticles (AuNPs) for the detection of E. coli O157:H7 and Salmonella typhimurium.Detects as low as 10(5) CFU/ml of target bacteria within 20 min or less, with 100% specificity.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0141 224445662012Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella Typhimurium and Staphylococcus aureusAptamer1TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50761)Whole cellDeveloped a luminescent bioassay for the simultaneous detection of S. Typhimurium and S. aureus using aptamer-conjugated MNPs and UCNPs (NaY0.78F4:Yb0.2, Tm0.02 UCNPs modified aptamer 1 and NaY0.28F4:Yb0.70, Er0.02 UCNPs modified aptamer 2).Display linear range of 10(1)-10(5) cfu/mL with a limit of detection of 5 cfu/mL for S. Typhimurium.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0157 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-1CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTG465'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0158 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-2ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCAT405'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0159 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-3GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGC395'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).Successfully detect S. typhimurium down to 600 CFU/mL (equivalent to 18 live cells in 30 μL of assay volume) and distinguish it from other Salmonella species, including S. enteritidis and S. choleraesuis.12Flow Cytometry25 nMDetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0160 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-4CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0161 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-5GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTCGCATAGGTAGTCCAGAAGCC615'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0162 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-6CTCCTCTGACTGTAACCACG-GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGCC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0163 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-7CTCCTCTGACTGTAACCACG-GTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGTC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0164 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-8CTCCTCTGACTGTAACCACG-GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0165 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-9CTCCTCTGACTGTAACCACG-ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCATC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0185 222263272012Bio-capture of S. Typhimurium from surface water by aptamer for culture-free quantificationSal aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-based bio-capture assay for the detection of selective serovar species through molecular-beacon-based real-time PCR.Achieved a detection limit of 1 CFU/PCR or 100 CFU/ml.N/AN/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0207 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-1_40GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGC395'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0208 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-6_60GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTCGCATAGGTAGTCCAGAAGCC615'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0209 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-12_80CTCCTCTGACTGTAACCACG-GTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGTC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0210 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-12_60CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT595'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium and 62 ± 12% (ST-12, 60nt) in S. typhimurium.12Flow Cytometry4.5 ± 0.4 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0211 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-20_80CTCCTCTGACTGTAACCACG-ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCATC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0212 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-20_40ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCAT405'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0213 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-33_60CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow Cytometry51.0 ± 4.3 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0214 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST2PATAGGAGTCACGACGACCAGAA-AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.The limit of detection (LOD) was 25 cfu/mL, and the percentage gated fluorescence intensity above library background was 20% when ST9P was incubated with L. monocytogenes cells and 72% when incubated with S. typhimurium.9Flow Cytometry6.33 ± 0.58 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0215 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST2AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA405'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 82 ± 2.34% for ST2.9Flow Cytometry16.34 ± 0.45 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0216 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST3PATAGGAGTCACGACGACCAGAA-TTTCGGCTAAGGACGGGTGAAATACATTTAATAGGGTGGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.N/A9Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0217 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST7PATAGGAGTCACGACGACCAGAA-TAAGTACAGGACTGGAGTATTAGCGGGGTCCATGCAAGGC-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.N/A9Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0218 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST9AATCAATAGAAGACAAAGTCCGAAACAGGTGTGACGGTAA405'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 72 ± 1.57% for ST9.9Flow Cytometry9.45 ± 0.63 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0219 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST9PATAGGAGTCACGACGACCAGAA-AATCAATAGAAGACAAAGTCCGAAACAGGTGTGACGGTAA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 66 ± 1.45% for ST9P.9Flow Cytometry11.14 ± 0.12 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0220 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC4CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGGCGTGGGGGCAATGCCTGCTTGTAGGCTTCCCCTGTGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.C4 showed strong specific binding to S. Typhimurium, and the increase in the fluorescence intensity of C4 was less than 30% against E. coli and S. aureus.10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0221 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC1CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGTCGGAGCCAGGATGCGAGGTCTGTAGGTCTGCGGGCGG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0222 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGCGGGCGAGTTGACGGCGTAATCGTGCTGCCGCGTG-GGTGGATCCATATTCCTACTCG995'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0223 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGGGCGTGGCTCAGAGTGGGGGTCGGTCAGTCTTGTGCGCG-GGTGGATCCATATTCCTACTCG1025'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0224 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC5CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGACGCCTGCGTGGCTGAGGCTTCGGTCTGCGCCG-GGTGGATCCATATTCCTACTCG1015'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0225 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC6CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CGTGCGGCGGGCAGGATGGGATGCTGTAGGTCTGCGGGCGCGCG-GGTGGATCCATATTCCTACTCG1045'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0226 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC7CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CGTGCGGAGCCAGGATGGGAGGTCTGTAGGTCTGCGGGGCGCG-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0227 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC8CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGGGACGGGTACCGGGCGTGTGGCGTGCCTGCCGCG-GGTGGATCCATATTCCTACTCG995'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0228 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC9CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGTGCGGGCCGGATGGGAGCTCTGTAGGTTGCGGGCGCGG-GGTGGATCCATATTCCTACTCG1015'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0229 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA1/SA-1CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGGGGCCAGGATGCGA-GGTGGATCCATATTCCTACTCG825'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0230 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA2/SA-2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GGTCTGTAGGTCTGCGGCGCGCG-GGTGGATCCATATTCCTACTCG835'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0231 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA3/SA-3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGGGGCCAGGATGCGAGGTCTGTAGGTCTGCGGCGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0273 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2GGGAGAGCGGAAGCGUGCUGGG-UAGUGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGA-CAUAACCCAGAGGUCGAUGGUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20.27 ± 4.372 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0274 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2 Truncated 43-merGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGAC395'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)27.64 ± 4.784 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0331 234946202013Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sortingS8-7GTATACGTATTACCTGCAGC-CTGATGTGTGGGTAGGTGTCGTTGATTTCTTCTGGTGGGG-CGATATCTCGGAGATCTTGC805'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNASalmonella Typhimurium (S. Typhimurium) (Strain S913)Whole cellIdentifying aptamers selected by whole-cell SELEX and developing a qPCR-based capture-detection platform for Salmonella Typhimurium.N/A10Flow Cytometry1.73±0.54 μMDetectionWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0332 234946202013Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sortingS8-46GTATACGTATTACCTGCAGC-CTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG-CGATATCTCGGAGATCTTGC805'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNASalmonella Typhimurium (S. Typhimurium) (Strain S913)Whole cellIdentifying aptamers selected by whole-cell SELEX and developing a qPCR-based capture-detection platform for Salmonella Typhimurium.Achieved a Lower Limit of Detection (LOD) of 10(2)-10(3) CFU in a 290-μl sample, and the mean capture efficiency ranged from 3.6% to 12.6%.10Flow Cytometry0.74±0.20 μMDetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0333 238720602013Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteriaA1GGGAACAGUCCGAGCCUCACUGUUAUCCGAUAGCAGCGCGGGAUGAGGGUCAAUGCGUCAUAGGAUCCCGC71N/AssRNASalmonella Typhi (CECT 409)Type IVB Pili structural proteinsDevelop a potentiometric biosensor based on carbon nanotubes (transducer layer) and aptamers (sensing layer) for the detection of bacteria.Display linear range from 0.2 CFU/mL (1 CFU in 5 mL PBS) to 10(6) CFU/mL of ST and LOD of 0.2 CFU/mL.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0337 242670762013A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labelsApt2ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-modified Quantum Dots (QD-apt) were developed for selective bacterial capture.For S. typhimurium, a linear range obtained between the concentrations 3.8 × 10(4) to 3.8 × 10(7) cfu/mL was obtained with QD 585-apt 2-FCM, and a detection limit of 5 × 10³ cfu/mL was achieved.N/AN/AN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0340 239293942013A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detectionS. enterica (FASE)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-functionalized graphene oxide (GO) nano-biosensor for multiplexed pathogen detection.Detection limits (LOD) of 61.0 cfu/mL (S. enterica) and linear ranges of 42.2–675.0 cfu/mL.N/AN/AN/ABiosensorN/A5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0413 244451152014An aptamer-based electrochemical biosensor for the detection of SalmonellaAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellAptamer-based electrochemical biosensor for Salmonella detection.A detection limit of 3 cfu/mL was achieved, with a linear range of 2.4 cfu/mL to 2.4 × 10^3 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0432 245686252014Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labelsApt₃ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a multiplex method for the simultaneous detection of three pathogenic bacteria using multicolour upconversion nanoparticles (UCNPs) coupled with aptamers.The linear range showed from 50-10(6) cfu/mL, and the limits of detection were 15 cfu/mL for S. typhimurium.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0433 246505832014A universal fluorescent aptasensor based on AccuBlue dye for the detection of pathogenic bacteriaST-aptAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA universal fluorescent aptasensor based on the AccuBlue dye was developed for the detection of pathogenic bacteria.Exhibit a linear concentration range of 50 to 10(6) cfu/mL, and the LOD was 25 cfu/mL in 1.5 h for S. typhimurium.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0454 https://doi.org/10.1016/j.foodcont.2013.09.0462014A visual detection method for Salmonella Typhimurium based on aptamer recognition and nanogold labelingAptamer 1TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (ATCC 50761)Whole cellDeveloped a sandwich detection method based on the recognition of aptamers coupled with nanogold labelling and silver signal amplification for the detection of Salmonella Typhimurium.Obtained a linear range of 10-10(6) cfu/mL, and the detection limit was observed to be 7 cfu/mL.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0455 https://doi.org/10.1016/j.foodcont.2013.09.0462014A visual detection method for Salmonella Typhimurium based on aptamer recognition and nanogold labelingAptamer 2TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (ATCC 50761)Whole cellDeveloped a sandwich detection method based on the recognition of aptamers coupled with nanogold labelling and silver signal amplification for the detection of Salmonella Typhimurium.Obtained a linear range of 10-10(6) cfu/mL, and the detection limit was observed to be 7 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0456 https://doi.org/10.1007/s00604-014-1170-42014Fluorescent aptasensor for the determination of Salmonella typhimurium based on a graphene oxide platformAptamerGGGAGCTCAGAATAAACGCTCAAGGGCAGGTGTTATGTGTACTGCTACAGTGTGGTTGTTCGACATGAGGCCCGGAC77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (CMCC 50115)Whole cellDeveloped a fluorescent aptasensor based on a graphene oxide platform for the determination of S. typhimurium.Displays a linear response to bacteria in the concentration range from 1 × 10(3) to 1 × 10(8) CFU/mL, with a detection limit of 100 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0458 251883922014Gold nanoparticle-based enzyme-linked antibody-aptamer sandwich assay for detection of Salmonella TyphimuriumSTM-binding aptamerATCCGTCACACCTGCTCTGGAGCAATATGGTGGAGAAACGTGGTGTTGGCTCCCGTAT58N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (CMCC 50115)Whole cellDevelop a gold nanoparticle-based enzyme-linked antibody-aptamer sandwich (nano-ELAAS) method for quantitative detection of STM.Quantitative detection range: 1 × 10³ to 1 × 10⁸ CFU/mL and LOD: 1 × 10³ CFU/mL, and a selectivity of >10-fold for STM in samples containing other bacteria at higher concentration, with an assay time of less than 3 h.N/ALPS-Aptamer Plate Binding Assay19.59 ± 0.35 nMBiosensorN/A5'-Amidation (NH₂-(CH2)6) and 5'-FITC Labeled and 5'-BiotinylatedN/AMMP-aptamers and nanoprobes were stored at 4°C for 2 weeks, no obvious change of the S/B was observed.N/AN/AN/A
ABdb_0462 https://doi.org/10.1007/s00604-014-1406-32014Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles.Apt2 (S. typhimurium aptamer)ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (ATCC 14028)Whole cellDevelop a dual fluorescence resonance energy transfer (FRET) from gQDs and rQDs and amorphous carbon nanoparticles (CNPs) for simultaneous detection of V. parahaemolyticus and S. typhimurium.LOD: 35 cfu/mL and linear range from 50 to 10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0465 250963912014Development of ssDNA aptamers for the sensitive detection of Salmonella typhimurium and Salmonella enteritidisSt1CCGATGTCCGTTAGGGCTCCTCCATAGAT295'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that can be used as detection probes in a polyvalent directed aptamer polymer (PDAP), which specifically bind to S. enteritidis and S. typhimurium.The binding efficiency of the PDAPs of St1 was approximately 20-fold higher than that of the single aptamer.10Fluorescence Spectroscopy0.530 ± 0.01 μM (of single aptamer) and 25 ± 5.6 nM (of PDAP)DetectionWhole Cell-SELEX5'-FAM Labeled, 3'-Thiolated and Poly-D-Lysine (PDL)N/AN/AN/AN/AN/A
ABdb_0466 244919612014Lateral flow biosensor for DNA extraction-free detection of Salmonella based on aptamer mediated strand displacement amplificationCapture-aptamer (c-aptamer)TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCCGAACAAGCTGAGGATGAAGAACAACGGCT89N/AssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (OMP)The gold-nanoparticle-based lateral flow biosensor was developed for the rapid detection of S. enteritidis.As low as 10(1) CFU of S. enteritidis per mL was detected.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0467 244919612014Lateral flow biosensor for DNA extraction-free detection of Salmonella based on aptamer mediated strand displacement amplificationAmplify-aptamer (a-aptamer)TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCCGAACAAGCTGAGGATGAAGAACAACGGCT89N/AssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (OMP)The gold-nanoparticle-based lateral flow biosensor was developed for the rapid detection of S. enteritidis.As low as 10(1) CFU of S. enteritidis per mL was detected.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0673 254762862015Aptamer-functionalized localized surface plasmon resonance sensor for the multiplexed detection of different bacterial speciesSty-aptTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 2421)Whole cellThe aptamer-immobilized LSPR sensor was developed for bacteria detection.Showed a detection limit of 30 cfu per assay.N/AN/AN/ABiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0675 https://doi.org/10.1007/s00604-015-1649-72015Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubesAnti-Salmonella aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50761)Whole cellDevelop a label-free impedimetric aptasensor based on an amino-modified aptamer bound to rGO-MWCNT composite for the detection of Salmonella.Exhibits a linear range from 75 to 7.5 × 10(5) cfu/mL and a detection limit of 25 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0700 262417352015Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellA GNPs-modified Raman-based biosensor was developed for the simultaneous detection of S. typhimurium and S. aureus.Linear detection range of 10^2 to 10^7 cfu/mL for both; LOD of 15 cfu/mL for S. typhimurium.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0702 254675002015Pathogen detection in complex samples by quartz crystal microbalance sensor coupled to aptamer functionalized core-shell type magnetic separationAptamerCTGATGTGTGGGTAGGTGTCGTTGATTTCTTCTGGTGGGG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an aptamer-based QCM biosensor for the determination of Salmonella in food samples.Showed specific detection of Salmonella cells at 100 CFU/mL from a milk sample and a linear response range from 100 to 4 × 10(4) CFU/mL cells.N/AQuartz Crystal Microbalance (QCM)3259 CFU mL−1BiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0704 https://doi.org/10.1039/C4AY02880E2015Rapid and sensitive detection of Salmonella typhimurium using aptamer-conjugated carbon dots as fluorescence probeS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop a fluorescence probe based on amino-modified aptamer conjugated to carboxyl-modified carbon dots (CDs) for detection of Salmonella typhimurium.Limit of Detection (LOD): 50 cfu mL⁻¹ and linear range of 10(3)-10(5) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0730 274242152016Gold nanoparticle-DNA aptamer conjugate-assisted delivery of antimicrobial peptide effectively eliminates intracellular Salmonella enterica serovar TyphimuriumAptHis (6H7)GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC40N/AssDNASalmonella typhimurium infected HeLa cellsS. typhimurium Whole cellHis-aptamer conjugated AuNP platform for the loading of His-tagged AMP (C-terminally hexahistidine-tagged A3-APO (A3-APOHis) AMPs) to eliminate intracellular S. Typhimurium cells.80% viability increase of infected HeLa cells and 100% survival rate of infected mice.N/AN/AN/ATargeted Delivery/TherapeuticsN/A3'-Thiolated (A10-Thiol) and Cyanine5 (Cy5) LabeledAuNP-Apt(His)-A3-APO(His) led to the increased viability of S. Typhimurium-infected HeLa cells.∼80% of A3-APOHis remained in serum after 48 h of incubation with AuNP-Apt(His)-A3-APO(His).N/AN/AN/A
ABdb_0731 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL26TAGCTCACTCATTAGGCA-CATTTGTGGCACCAAATTTGAATTAATCAAGACAGTGTGGT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.SAL 26 can detect 10(2) CFU/mL in the gold nanoparticle-based colourimetric assay and has a limit of detection (LOD) of 10(3) CFU/mL in the aptamer sedimentation assay.10Fluorescence Spectroscopy123 ± 23 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0732 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL33TAGCTCACTCATTAGGCA-CTCGTATTGAGGAGCTCGTATTATTTAATTCACACTGTGCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence Spectroscopy221 ± 72 nMBiosensorWhole Cell-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0733 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL28TAGCTCACTCATTAGGCA-CTCTGTCGTCAACAGTCTAACTTGTAGAATTGATGTTACTG-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.Limit of detection (LOD) of 10(3)CFU/mL in the aptamer sedimentation assay.10Fluorescence Spectroscopy195 ± 46 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0734 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL43TAGCTCACTCATTAGGCA-CGAGACCACCGGCAACCTTTCACAAAGGGGAGGCTAA-GCATAGTTAAGCCAGCC725'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence Spectroscopy294 ± 112 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0735 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL21TAGCTCACTCATTAGGCA-CAGCAACTCGCTTTGATACCGGTAGAGTTTGTATTGCTCAA-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0736 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL37TAGCTCACTCATTAGGCA-CTTAGTTCAGACGCGAGTATTATCGGCGTCCAAGCAAGGG-GCATAGTTAAGCCAGCC755'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0737 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL20TAGCTCACTCATTAGGCA-CATATTCAGCAGTTGTATGATAGCGGGTCGCGAGTGTGTAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0738 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL40TAGCTCACTCATTAGGCA-CTGCATTACATATATGGCAGTGGGTTAACCTGGATTGAGGA-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence Spectroscopy204 ± 73 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0739 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL16TAGCTCACTCATTAGGCA-CCCGATTGTACTAATGAAGATGGACACTGAGAGGGGGATGG-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0740 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL11TAGCTCACTCATTAGGCA-CTCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.Limit of detection (LOD) of 10(3)CFU/mL in the aptamer sedimentation assay.10Fluorescence Spectroscopy184 ± 43 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0741 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL45TAGCTCACTCATTAGGCA-CCGTGCCAACTGAGGGATACAGGGGGAGTGTCGGTCTACCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence Spectroscopy294 ± 133 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0742 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL23TAGCTCACTCATTAGGCA-CCTCACAGCCCTTCTTCCCGTATTTGTAGACTAGTTACATT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0743 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL48TAGCTCACTCATTAGGCA-CCACACTGTCTTGATTTTGGATTTGTCGGTGCTGACCTGTG-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0744 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL9TAGCTCACTCATTAGGCA-CTGCGAGTGAACTCCTCCTTTTGTATTTAGTGTGCGGATCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0745 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL29TAGCTCACTCATTAGGCA-CATGTGGAGTGCGAACTGTCTGGTCTTATCGGGCTCGCTGT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0746 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB5CAGTCCAGGACAGATTCGCGAG-CCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATC-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.LOD was 7.9 × 10(3) CFU/mL and a linear range from 7.9 × 10(2) to 7.9 × 10(6) CFU/mL of S. typhimurium with less than 1 h.10Quartz Crystal Microbalance (QCM)58.5 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0747 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumA24CAGTCCAGGACAGATTCGCGAG-GCACAATCCACCTCTCACCGCACGCCACGCACTGCCTCTGTCCCG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)19.63 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0748 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB30AATCGCTGAATGAAATCCACGTG-GGAAGTGTGTGGGTGACCAGAGGTGTGGTGATGGGATTGTCGTA-CCTCGCGAATCTGTCCTGGACTG905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)51.02 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0749 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB31CAGTCCAGGACAGATTCGCGAG-CCGCACCGCCCTATCCTCGTGTCCCTGCCCAACAGCGCCGCCCGC-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0750 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumA37CAGTCCAGGACAGATTCGCGAG-GTGGGGCGGGAAAGTGTGTGTAGGTGGTGGCGTTGTCACGGCGGG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0751 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB16CAGTCCAGGACAGATTCGCGAG-GGGCTGTGTGAGAAGTGGGTGTGGTGGTGGATATGCAACGTTGTA-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0752 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB21CAGTCCAGGACAGATTCGCGAG-GTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0806 265998602016Salmonella typhimurium detection using a surface-enhanced Raman scattering-based aptasensorApt1AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA SERS-based aptasensor bearing Au@Ag core/shell nanoparticles (NPs) functionalized with apt 1 and X-rhodamine (ROX)-modified apt 2 was developed for the detection of bacteria.A calibration curve is obtained in the range of 15 to 1.5 × 10(6) CFU/mL with a limit of detection of 15 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0807 265998602016Salmonella typhimurium detection using a surface-enhanced Raman scattering-based aptasensorApt2AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA SERS-based aptasensor bearing Au@Ag core/shell nanoparticles (NPs) functionalized with apt 1 and X-rhodamine (ROX)-modified apt 2 was developed for the detection of bacteria.A calibration curve is obtained in the range of 15 to 1.5 × 10(6) CFU/mL with a limit of detection of 15 CFU/mL.N/AN/AN/ABiosensorN/A5'-X-rhodamine (ROX) modifiedN/AN/AN/AN/AN/A
ABdb_0808 268949872016Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sampleAptamer (N45)TTTGGTCCTTGTCTTATGTCCAGAATGCGAGGAAAGTCTATAGCAGAGGAGATGTGTGAACCGAGTAAATTTCTCCTACTGGGATAGGTGGATTAT96N/AssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (OMP)Developed a label-free impedimetric aptamer-based biosensor for S. typhimurium detection.Rapid (30 min) detection with a concentration range of 1×10(1) to 1×10(8) CFU/mL and limit of detection (LOD) of 6 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0816 270637162016Magnetic Nanoparticles-based Aptasensor Using Gold Nanoparticles as Colorimetric Probes for the Detection of Salmonella typhimuriumS. typhimurium aptamerATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor for S. typhimurium detection using gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs).The assay shows a linear response over a range of 25 to 10(5) cfu/mL, and the detection limit was improved to 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (for Apt1) and 5'-Biotinylated (for Apt2)N/AN/AN/AN/AN/A
ABdb_0818 269712742016Dual-excitation upconverting nanoparticle and quantum dot aptasensor for multiplexed food pathogen detectionS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDual-excitation aptasensing platform based on the luminescent nanoparticles (QDs and UCNPs) for the detection of Salmonella typhimurium and Staphylococcus aureus.Linear detection range: 50 to 10⁶ cfu/mL; LOD: 28 cfu/mL (S. typhimurium).N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0822 https://doi.org/10.1039/C6AY02623K2016SERS aptasensor detection of Salmonella typhimurium using a magnetic gold nanoparticle and gold nanoparticle based sandwich structureS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50761)Whole cellDeveloped a SERS aptasensor using aptamer-functionalized magnetic gold nanoparticles (MGNPs) and mercaptobenzoic acid (4-MBA) functionalized gold nanoparticles (GNPs) based sandwich structure for the detection of S. typhimurium.Exhibit a good linear range from 10 cfu/mL to 10(7) cfu/mL, and the LOD is 5 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0952 https://doi.org/10.1016/j.foodcont.2017.07.0162017SERS aptasensor for Salmonella typhimurium detection based on spiny gold nanoparticlesS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a novel SGNPs-based SERS aptasensor functionalized with p-MBA for the detection of S. typhimurium.Display linear range 10(1) cfu/mL to 10(5) cfu/mL, with a limit of detection (LOD) of 4 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated or BiotinylatedN/AN/AN/AN/AN/A
ABdb_0958 281076862017A novel aptasensor for the colorimetric detection of S. typhimurium based on gold nanoparticlesS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellColorimetric aptasensor was developed based on color changing GNPs for the detection of S. typhimurium.Quantitative detection of S. typhimurium from 10(2) to 10(7) cfu/mL with a detection limit as low as 56 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0959 281079302017A chemiluminescent aptasensor based on rolling circle amplification and Co2+/N-(aminobutyl)-N-(ethylisoluminol) functional flowerlike gold nanoparticles for Salmonella typhimurium detectionS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellChemiluminescent aptasensor based on rolling circle amplification (RCA) using Co2+/ABEI-AuNFs-cDNA complex was developed for the detection of S. typhimurium.The limit of detection (LOD) was 10 cfu/mL. The method exhibited excellent selectivity over non-target bacteria.N/AN/AN/ABiosensorN/A3'-Biotinylated (Biotin-TTTTTT)N/AN/AN/AN/AN/A
ABdb_0960 290306712017Graphene-based label-free electrochemical aptasensor for rapid and sensitive detection of foodborne pathogenS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellrGO-azophloxine (AP) nanocomposite aptasensor was developed for the detection of foodborne pathogens.Exhibited a linear range of detection from 10(8) to 10(1) cfu/mL with a detection limit of 10(1) cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AAptasensor showed good stability for up to 20 days, with a 5.1% increase in current response when stored in ultrapure water at 4°C.N/AN/AN/A
ABdb_0961 286457562017An aptamer-based PCR method coupled with magnetic immunoseparation for sensitive detection of Salmonella Typhimurium in ground turkeyS. Typhimurium aptamerCAGTCCAGGACAGATTCGCGAGCCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATCCACGTGGATTTCATTCAGCGATT90N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-based PCR method coupled with magnetic immunoseparation was developed to detect S. Typhimurium from ground turkey.Able to detect 10(2) CFU/mL of S. Typhimurium in pure culture, and 10(3) CFU/mL of S. Typhimurium in ground turkey.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0972 289106782017Colorimetric aptasensor for the detection of Salmonella enterica serovar typhimurium using ZnFe2O4-reduced graphene oxide nanostructures as an effective peroxidase mimeticsApt 1 (Capture)AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellThe colorimetric aptasensor platform was developed based on ZnFe2O4/rGO nanostructures, which act as an artificial enzyme (mimetic) that catalyses the oxidation of TMB by H2O2 to detect S. typhimurium.The limit of detection (LOD) of 11 cfu/mL, and the detection range was 11 to 1.10 × 10(5) cfu/mL of S. typhimurium in buffer solution.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0973 289106782017Colorimetric aptasensor for the detection of Salmonella enterica serovar typhimurium using ZnFe2O4-reduced graphene oxide nanostructures as an effective peroxidase mimeticsApt 2 (Signal)AAAAAAAAAAAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA52N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellThe colorimetric aptasensor platform was developed based on ZnFe2O4/rGO nanostructures, which act as an artificial enzyme (mimetic) that catalyses the oxidation of TMB by H2O2 to detect S. typhimurium.Limit of detection (LOD) of 11 cfu/mLN/AN/AN/ABiosensorN/A5'-PolyA (12A)N/AN/AN/AN/AN/A
ABdb_0976 288604622017Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meatAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 2421)Whole cellDevelop a portable LSPR biosensor-based device on gold nanoparticles (AuNPs) for the detection of Salmonella typhimurium in pork meat samples.Limit of detection (LOD) of 10(4) cfu/mL in both pure culture and spiked pork samples, and total analysis time of 30-35 minutes.N/AN/AN/ABiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0978 275263402017Aptamer biosensor for Salmonella typhimurium detection based on luminescence energy transfer from Mn2+-doped NaYF4:Yb, Tm upconverting nanoparticles to gold nanorodsS. typhimurium aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellA LET method based on UCNPs and gold nanorods (Au NRs) was developed for the determination of Salmonella typhimurium.Linear range: 12 to 5×10^5 cfu/mL and Limit of Detection (LOD): 11 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0997 https://doi.org/10.1007/s12161-017-0864-82017Fabricating a Novel Raman Spectroscopy-Based Aptasensor for Rapidly Sensing Salmonella typhimuriumS. typhimurium aptamer (STA)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a surface-enhanced Raman scattering (SERS) based aptasensor with Raman active molecule attached GNRs and cDNA for the detection of Salmonella typhimurium (ST).Observed linear ST concentration from 56 to 56 × 10(7) cfu/mL with a LOD of 9 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1000 https://doi.org/10.1016/j.jelechem.2017.10.0542017Development of an aptasensor using reduced graphene oxide chitosan complex to detect SalmonellaAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a rGO-CHI electrochemical aptasensor to detect Salmonella.Had a wide detection range of 10(1) to 10(6) CFU/mL with a low limit of detection of 10(1) CFU/mL.N/AN/AN/ABiosensorN/A3'-Thiolated ((C12)-SH) and 5'-Amidation ((C12)-NH₂)N/AN/AN/AN/AN/A
ABdb_1002 293574052018Combat biofilm by bacteriostatic aptamer-functionalized graphene oxideST-3 (or ST-33)CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG60N/AssDNASalmonella Typhimurium (S. Typhimurium)BiofilmAptamer-GO conjugate reduces the cellular membrane potential to inhibit biofilm formation.93.3 ± 3.4% inhibition ratio at the initial stage of biofilm formation and 84.6 ± 5.1% degradation ratio on formed biofilms.12N/AN/ATherapeuticsN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1066 298966412018Aptamer based SERS detection of Salmonella typhimurium using DNA-assembled gold nanodimersAptamer (ssDNA1)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDevelop SERS-based aptasensor using DNA-assembled gold nanodimers (AuNDs) for detection of S. typhimurium.Display a linear range in the 10(2) to 10(7) cfu/mL, and the limit of detection is 35 cfu/mL within 1hr.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1083 https://doi.org/10.1002/slct.2018010082018Exploiting Stokes and anti-Stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applicationsS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellaptamer-functionalized QD and UCNP nanoprobes that were conjugated with partially complementary DNA-modified magnetic beads for separation.The limit of detection was 12 cfu/mL for S. typhimurium with a linear range from 10(2)-10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1124 298706922018Carbon nanotube-based aptasensor for sensitive electrochemical detection of whole-cell SalmonellaSalmonella aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an amino-modified aptasensor using MWCNTs-deposited ITO electrode for the detection of Salmonella.It possesses a linear range from 10 mV/s to 90 mV /s, and the detection limit was 5.5 × 10(1) cfu/mL and 6.7 × 10(1) cfu/mL for S. Enteritidis and S. Typhimurium, respectively.N/AN/AN/ABiosensorN/AAmidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1126 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS1GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGCTGGTGGCCATTGGCTCGTGTCTCGTTACTGCCGAGTG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.Exhibited a linear concentration ranging from 2×10(1) to 2×10(5) CFU/mL and LOD of 20 CFU/mL.10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1127 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS2GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GGAATGACCTGGATTGACCCGTGAGTGTAGCATTTGTCCG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1128 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS3GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TTGACGATACGCAGGGGCGATACAGACAGTCTGCGTATTC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1129 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS4GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CTGTATCCCTCCTCGCTTCCATACCCAAACCGGACACACC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1130 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS5GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CTGCATGCACCTCACGCATCGAGCACTCACTCCTTCTACG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1131 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS6GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CCTCCCGAGGACACACACACACCATCGCGGTACTCTCCCG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1132 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS7GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GCGCACCTCCGCCCTGTCGGGCGTCGTTTCCCCACGAATC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1133 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS8GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GTTTCCGTATGCGCAGCGCCATACACCTGTCTGATCTCCCC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1134 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS9GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGTCGCAAGGACTCGATCTGGTGGTACCTGCTTTCCGTTG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1135 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS10GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGAGCACCAAGGATAGTTAGCGGTCGCCTTCACACTAGGC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1136 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS11GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CTGACAGATTAAAAGTGGTTGGGCAACTTCTGCTTGCGAA-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)4.41 × 10(-12) MDetectionWhole Cell-SELEX5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1137 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS12GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GGTGGTGTTGACAATTGAACCTTTGCAGGGGTCTGGTGGG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1138 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS13GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GTGGAGGTGCGCTATAGGTCTCTCCGGGTACTGATCCAAT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1139 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS14GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CCGACCCGTAACGCCAGTGAGGGAACACTCTGGAATAGTT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1140 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS15GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CGAGCAAACTGATAAACTCCGCCATGTCAGGCAAAGCTTT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1141 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS16GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CTTCGTTCCGTTGACATAATTCAATTTTGCGTATTTCTTG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1142 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS17GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-ATGGGGCTAGTCTACTTCTGTGTCTACCTAGGATTCCGCT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1143 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS18GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGTAGGCGTAACCTCCTACTCTGTGCACTGTCTAAAGTGAC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1144 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS19GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CGCGGCGTACGCTATCTTGTAACCTCCCGAACTATAACCAC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1145 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS20GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CAAGCGACTCGACCGTGGTACCTCCCCAATAGCCATTTGA-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1146 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS21GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TCTCGCAGCCTCTTCCAATCTTGTTATTTACGCTTTCTGT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1147 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS22GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CACAAAGGAGCAAGAACTCAATTGCGCTCGTACCTTGTGG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1148 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS23GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GTAGCTGGGGTCCGGATCTGCGGTTCAGTGCGCTATTGTG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1149 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS24GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGACGGCTATCTCATTTGGCACATCATTTAGTAAGCTATC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)3.75 ×10(-3) MDetectionWhole Cell-SELEX5'-Cyanine5 (Cy5) Labeled or 5′-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1196 300810312018Label-free aptasensors based on fluorescent screening assays for the detection of Salmonella typhimuriumS. typhimurium binding Aptamer (Apt1)CCAAAGGCTACGCGTTAACGTGGTGTTGG29N/AssDNASalmonella Typhimurium (S. Typhimurium) (MTCC 3232)Whole cellA label-free fluorescent aptasensor was developed for the detection of Salmonella typhimurium using SYBR Green I.Achieved a linear range of approximately 1530-96938 CFU/mL and a detection limit of 733 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1197 300810312018Label-free aptasensors based on fluorescent screening assays for the detection of Salmonella typhimuriumS. typhimurium binding Aptamer (Apt2)ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (MTCC 3232)Whole cellAn aptasensor based on FRET between Rhodamine B and gold nanoparticles for the detection of Salmonella typhimurium was developed.Achieved concentrations ranging from 1530 to 96938 CFU/mL, with a detection limit of 464 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1198 303595192018Ω-Shaped Fiber-Optic Probe-Based Localized Surface Plasmon Resonance Biosensor for Real-Time Detection of Salmonella TyphimuriumAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-immobilized Ω-shaped fiber-optic localized surface plasmon resonance (FOLSPR) biosensor for S. Typhimurium detection.Achieved high detection sensitivity for S. Typhimurium down to 128 CFU/mL within a linear range from 5 × 10(2) to 1 × 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1199 https://doi.org/10.1016/j.snb.2017.08.1602018Nanoporous gold as a suitable substrate for preparation of a new sensitive electrochemical aptasensor for detection of Salmonella typhimuriumAnti-S. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a label-free electrochemical aptasensor based on nanoporous gold (NPG/Au/GCE) for the detection of Salmonella typhimurium.Capable of detecting S. typhimurium in a wide linear dynamic range 6.5 × 10(2) to 6.5 × 10(8) CFU/mL with a LOD of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1202 300014872018Magnetism-Resolved Separation and Fluorescence Quantification for Near-Simultaneous Detection of Multiple PathogensS. typ aptamerATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-modified fluorescent-magnetic multifunctional nanoprobes (apt-FMNPs) for near-simultaneous detection of multiple pathogens.The detection limits of 150 cfu/mL in milk, 120 cfu/mL in human serum, and 120 cfu/mL in human urine for S. typ.N/AN/AN/ABiosensorN/A5'-Biotinylated and 3'-FAM LabeledBacterial viability remained about 100% during incubation with apt-FMNPs nanoprobes at successive incubation time points of 0, 0.5, 1, 3, 6, 12, and 18 h.N/AN/AN/AN/A
ABdb_1225 312163062019Aptamer-based fluorometric determination of Salmonella Typhimurium using Fe3O4 magnetic separation and CdTe quantum dotsAptamer (ssDNA1)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Outer membrane protein (OMP)Develop an aptamer-based (Apt-MNPs) fluorescence assay for the determination of Salmonella Typhimurium.Exhibit a linear range of 10 to 10(10) cfu/mL with LOD of 1 cfu/mL within 2 h, and is successfully applied to the analysis of spiked food samples with good recoveries from 90% to 105%.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_1245 308256572019A reduced graphene oxide-titanium dioxide nanocomposite based electrochemical aptasensor for rapid and sensitive detection of Salmonella entericaS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a rGO-TiO2 electrochemical aptasensor for the detection of Salmonella bacteria.Exhibited high sensitivity with a wide detection range (10(8) to 10(1) cfu/mL), a low detection limit of 10(1) cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AShowed good durability up to 21 days with a 10% decrease in signal after 30 days of non-use.N/AN/AN/A
ABdb_1247 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA27CCTTCTAACAGAATGTTGTTAGATAGC27N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.Detection limits (3σ/S) obtained within 30 minutes using LA27 with respect to LPS, Pa-LPS, and Ecoli-LPS were 38.7, 88.0, and 154 ng/mL, respectively.N/AIsothermal Titration Calorimetry (ITC)46.2 ± 9.5 nMBiosensorN/A5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1248 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA80ATAGGAGTCACGACGACCAGCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTCTATGTGCGTCTACCTCTTGA80N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.Detection limits (3σ/S) obtained using LA80 with respect to LPS, Pa-LPS, and Ecoli-LPS were 0.185, 2.56 and 2.82 μg/mL, respectively.N/AIsothermal Titration Calorimetry (ITC)102 ± 17 nMBiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1249 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA60TCACGACGACCAGCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTCTATGTGC60N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1250 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA54CGACGACCAGCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTCTATG54N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1251 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA48CGACCAGCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTCT48N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1252 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA40CCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTC40N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1253 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA36GCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGC36N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.LA36 has good specificity.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1254 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA22TTCTAACAGAATGTTGTTAGAT22N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1329 314105762019Electrochemical determination of Salmonella typhimurium by using aptamer-loaded gold nanoparticles and a composite prepared from a metal-organic framework (type UiO-67) and grapheneAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellAn aptamer-based assay based on a metal-organic framework-graphene composite of type UiO-67/GR and an aptamer-gold nanoparticles-horseradish peroxidase (Apt-AuNP-HRP) conjugate is described for the determination of S.typhimurium.Linear detection range from 2 × 10(1) – 2 × 10(8) cfu/mL and a lower detection limit of 5 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/A91.3% of the original current is retained after storage at 4°C for 14 days with the modified electrodes, allowing monitoring of 2 × 10(5) cfu/mL S. typhimurium.N/AN/AN/A
ABdb_1438 320706112020Rapid and sensitive detection of Salmonella Typhimurium using nickel nanowire bridge for electrochemical impedance amplificationAptamerCAGTCCAGGACAGATTCGCGAGCCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATCCACGTGGATTTCATTCAGCGATT90N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an electrochemical aptasensor using an aptamer-coated gold interdigitated microelectrode and antibody-modified NiNWs to detect Salmonella typhimurium.This electrochemical aptasensor quantitatively detected Salmonella at concentrations ranging from 10(2) to 10(6) CFU/mL within 2 h, with a detection limit of 80 CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1440 316550502020Rapid and sensitive detection of Salmonella with reduced graphene oxide-carbon nanotube based electrochemical aptasensorS. Typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop a biosensor using reduced graphene oxide-carbon nanotubes (rGO-CNT) nanocomposite via the hydrothermal method for label-free electrochemical detection of S. enterica.Display a wide linear dynamic range from 10(1) until 10(8) cfu/mL with a 10(1) cfu/mL of the limit of detection.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AssDNA/rGO-CNT/GCE aptasensor showed good to excellent stability when stored for 20 days in ultrapure water at 4°C.N/AN/AN/A
ABdb_1454 332417942020Rapid and highly sensitive detection of Salmonella typhimurium in lettuce by using magnetic fluorescent nanoparticlesS. typhimurium aptamerAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Outer membrane protein (OMP)Develop a fluorescent sensor (FMNCs-Apt), based on Fe3O4 magnetic nanoparticles and aptamer-modified carbon quantum dots for the detection of S. typhimurium in lettuce.Detection limit (LOD) of 100 CFU/mL in vegetable washing solution and 138 CFU/mL in lettuce samples with a linear range of 10(3)-10(6) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1497 https://doi.org/10.1016/j.foodcont.2020.1072812020A novel AuNPs colorimetric sensor for sensitively detecting viable Salmonella typhimurium based on dual aptamersApt1GAGGAAAGTCTATAGCAGAGGAGATGTGTGAACCGAGTAA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor based on AuNPs for visual detection of viable S. typhimurium.Showed a linear range from 3.3 × 10(1) to 3.3 × 10(6) CFU/mL and the detection limit of 33 CFU/mL in pure culture and 95 CFU/mL in spiked milk.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1498 https://doi.org/10.1016/j.foodcont.2020.1072812020A novel AuNPs colorimetric sensor for sensitively detecting viable Salmonella typhimurium based on dual aptamersApt2CTCCTCTGACTGTAACCACGGAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGCCGCATAGGTAGTCCAGAAGCC80N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor based on AuNPs for visual detection of viable S. typhimurium.Showed a linear range from 3.3 × 10(1) to 3.3 × 10(6) CFU/mL and the detection limit of 33 CFU/mL in pure culture and 95 CFU/mL in spiked milk.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1501 320000582020A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrateApt 2AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a SERS aptasensor using Apt-Au-PDMS film for simultaneous multiple-pathogen detection.Can selectively detect 27 cfu/mL cells.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1540 346649412021Efficient Eradication of Bacterial Biofilms with Highly Specific Graphene-Based Nanocomposite SheetsS. Typhimurium aptamer (ST-NH2)TTTTTAAGCCCACTGGCGTTCGGACATCACAGCTCGTGCAGGTCGTGCCATG52N/AssDNASalmonella Typhimurium (S. Typhimurium) (CMCC 50115)Whole cellICG@GO-Apt nanosheets for eradication of biofilm associated with Salmonella Typhimurium.Shows an efficient biofilm elimination with an efficiency of greater than 99.99% in an abscess formation model.N/AN/AN/ATargeted Delivery/TherapeuticsN/A3'-Amidation (NH₂) or TAMRA, 5'-FAM Labeled or Amidation (NH₂)ICG@GO-Apt NSs had almost no cytotoxicity to HEK 293 cells, with more than 90% and lacked hemolytic activities.N/AN/AN/AN/A
ABdb_1541 332851252021An aptamer biosensor based dual signal amplification system for the detection of salmonella typhimuriumS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ASI 1174)Whole cellDeveloped a signal-cascade-amplification method named “immuno-HCR-SERS” for the detection of S. typhimurium.Highly sensitive detection showing a linear range from 10 to 10(5) CFU/mL, with a limit of detection (LOD) of 6 CFU/mL in 3.5 h.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1542 348288202021A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of Salmonella typhimurium in MilkCapture aptamer (Bapt)GAGGAAAGTCTATAGCAGAGGAGATGTGTGAACCGAGTAA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colourimetric assay based on aptamer-catalysed hairpin assembly signal amplification strategy (Y-CHA ) for the detection of S. typhimurium in milk.Exhibited a concentration range of 10(2) to 10(6) CFU/mL, with a sensitivity of 2.4 × 10(2) CFU/mL under optimal conditions and 2.8 × 10(3) CFU/mL in real milk samples.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1543 348288202021A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of Salmonella typhimurium in MilkTarget aptamer (Tapt)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colourimetric assay based on aptamer-catalysed hairpin assembly signal amplification strategy (Y-CHA ) for the detection of S. typhimurium in milk.Exhibited a concentration range of 10(2) to 10(6) CFU/mL, with a sensitivity of 2.4 × 10(2) CFU/mL under optimal conditions and 2.8 × 10(3) CFU/mL in real milk samples.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1549 330760892021Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimuriumAptamer1AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor using gold nanoclusters (aptamers@BSA-AuNCs) for the detection of S. typhimurium.Exhibited a wide linear range of 10(1)-10(6) cfu/mL with a detection limit as low as 1 cfu/mL.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1550 330760892021Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimuriumAptamer2TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor using gold nanoclusters (aptamers@BSA-AuNCs) for the detection of S. typhimurium.Exhibited a wide linear range of 10(1)-10(6) cfu/mL with a detection limit as low as 1 cfu/mL.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1563 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA6GGGCAGGTGTTATGTGTACTGCTACAGTGTGGTTG35N/AssDNASalmonella Typhimurium (S. Typhimurium) (CICC 21484)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Show a linear range for monoaptasensor in the range of 1 × 10(1) to 1 × 10(9) CFU/mL and for polyaptasensor ranging from 1 × 10(1) to 1 × 10(7) CFU/mL with an LOD of 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1564 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA16GGCAGGACAACAGCGTGTAGTATCAGCTTACGGTG35N/AssDNASalmonella Typhimurium (S. Typhimurium) (CICC 21484)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Show a linear range for monoaptasensor in the range of 1 × 10(1) to 1 × 10(9) CFU/mL and for polyaptasensor ranging from 1 × 10(1) to 1 × 10(7) CFU/mL with an LOD of 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1565 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA18PCCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG35N/AssDNASalmonella Typhimurium (S. Typhimurium) (CICC 21484)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Show a linear range for monoaptasensor in the range of 1 × 10(1) to 1 × 10(9) CFU/mL and for polyaptasensor ranging from 1 × 10(1) to 1 × 10(7) CFU/mL with an LOD of 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1580 https://doi.org/10.1016/j.snb.2021.1303372021Electrochemical aptasensor for simultaneous detection of foodborne pathogens based on a double stirring bars-assisted signal amplification strategyApt-S.TTGGCTAGCTCAGTCATATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAGCCGCG60N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a double stirring bar-based signal-amplified strategy using aptamer-embedded tetrahedral DNA nanostructures for the simultaneous electrochemical detection of Vibrio parahaemolyticus (V.P) and Salmonella typhimurium (S.T).Display a lower detection limit of 7 CFU/mL for S.T. within a detection range of 10–10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1587 335903782021Sensitive colorimetric aptasensor based on g-C3N4@Cu2O composites for detection of Salmonella typhimurium in food and waterAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA colorimetric aptasensor using graphitic carbon nitride (g-C3N4) nanosheets and copper oxide(I) (Cu2O) nanocrystals was developed for detecting S. typhimurium.Exhibited linear range from 1.5 × 10(1) to 1.5 × 10(5) CFU/ml, with a detection limit of 15 CFU/ml.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1592 338949562021A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognitionS. typhi-aptamerATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium)Staphylococcus aureus Protein A (SpA)Developed a SERS aptasensor using aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) and PA modified-SERS tags (Au@DTNB@PA) for the detection of bacteria.The limit of detection (LOD) for S. typhi was 25 cells/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1593 340415802021Fabrication of gold/silver nanodimer SERS probes for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an aptamer-facilitated gold/silver nanodimer SERS probe for the simultaneous detection of the two bacteria with the help of magnetic separation enrichment.Achieved a linear range of 10(2) to 10(7) cfu/mL, with a detection limit of 50 cfu/mL.N/AN/AN/ABiosensorN/A5′-SH-A-Cy3 and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1598 10.33263/BRIAC112.870287152021Novel Competitive Voltammetric Aptasensor Based on Electrospun Carbon Nanofibers-Gold Nanoparticles Modified Graphite Electrode for Salmonella enterica serovar DetectionAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an electrochemical aptasensor based on aptamer-linked GNPs/CNF-Chi/GEs for the detection of Salmonella enterica Serovar.Exhibited a linear range of 10 to 10(5) CFU/mL with the limit of detection (LOD) 1.223 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1715 354482892022Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride NanosphereS. typhimurium AptTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDevelop an off-on fluorescence aptasensor for the detection of S. typhimurium and use HCNS-Cap for disinfection.The fluorescence assay showed a linear range of 30 to 3 × 10(4) CFU/mL and a detection limit of 13 CFU/mL. The bactericidal efficiency of HCNS-Cap (95.0%) within 12 h was better than that of HCNS (85.1%) and Cap (72.9%).N/AN/AN/ADiagnostic/TherapeuticsN/ACyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1716 362058282022Aptamer-AuNP-conjugated carboxymethyl chitosan-functionalized graphene oxide for colorimetric identification of Salmonella typhimuriumS. typhimurium AptamerGCGCTCGGCCTCCTCTGCCATCTCATTCGCGAGCC35N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop a novel aptamer-AuNP-conjugated carboxymethyl chitosan–functionalized graphene oxide (CMC/GO@Apt-Au NP) probe for the determination of S. typhimurium.Achieved a linear range from 10(2) to 10(7) CFU/mL with a limit of detection (LOD) of 10 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AThe relative response of aptasensor decreased to ~ 77% within 11 days of storage.N/AN/AN/A
ABdb_1717 346934712022Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12aAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellAn electrochemical biosensor platform based on HCR-based CRISPR-Cas12a for specific detection of S. typhimurium.Selectively and sensitively quantify S. typhimurium in samples with detection limits of 20 CFU/mL and a linear range of 10-10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1744 352752702022DNAzyme-controlled plasmonic coupling for SERS-based determination of Salmonella typhimurium using hybridization chain reaction self-assembled G-quadruplexAptAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 1925)Whole cellDeveloped a SERS aptasensor based on hybridization chain reaction (HCR) self-assembled G-quadruplex DNAzyme (GQH DNAzyme)-controlled plasmonic coupling for S. typhimurium detection.Display a linear range from 5 to 5.0 × 10(5) cfu/mL and the limit of detection (LOD) of 4 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1792 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt (mono-apt)GAGAGAGAATATAAGGGAAAAAAAAAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG82N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.N/AN/AEnzyme-Linked Immunosorbent Assay (ELISA)389.63 nM (with mono-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1793 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt1 (multi-apt)GAAGTGTACGTAGCCTGATTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1794 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt2 (multi-apt)TCGTCTACACTACTGCTCTTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1795 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt3 (multi-apt)TCTCCAATGACGTACCTGTTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1796 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt4 (multi-apt)GTTGCAGTACTCTACGAGTTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1797 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt5 (multi-apt)TGTACTGGACAAGTGACGTTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1812 378937442023A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent AptamerApt1GAAGTGTACGTAGCCTGATTCTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG60N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric/fluorescent dual-mode method based on a DNA-nanotriangle programmed multivalent aptamer (NTri-Multi-Apt) for the detection of Salmonella.Achieved a linear range of 1.0 × 10(2)–1.0 × 10(7) CFU/mL and LODs of 316 and 60 CFU/mL for colorimetric and fluorescent detection, respectively.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.89 nMBiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1813 378937442023A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent AptamerApt2TACTGCTCTTCTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG50N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric/fluorescent dual-mode method based on a DNA-nanotriangle programmed multivalent aptamer (NTri-Multi-Apt) for the detection of Salmonella.Achieved a linear range of 1.0 × 10(2)–1.0 × 10(7) CFU/mL and LODs of 316 and 60 CFU/mL for colorimetric and fluorescent detection, respectively.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.89 nMBiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1814 378937442023A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent AptamerApt3CGTACCTGTTCTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG50N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric/fluorescent dual-mode method based on a DNA-nanotriangle programmed multivalent aptamer (NTri-Multi-Apt) for the detection of Salmonella.Achieved a linear range of 1.0 × 10(2)–1.0 × 10(7) CFU/mL and LODs of 316 and 60 CFU/mL for colorimetric and fluorescent detection, respectively.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.89 nMBiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1821 367057342023A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS techniqueS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an assembled aptasensor based on Fe3O4@Au@Ag nanocomposites for the detection of various pathogenic bacteria.Showed a linear range of 10-10(7) CFU/mL, with a detection limit of 2.27 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1827 373112992023Joint concanavalin A-aptamer enabled dual recognition for anti-interference visual detection of Salmonella typhimurium in complex food matricesS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA dual recognition platform based on Concanavalin A (Con A)-aptamer joint strategy for sensitive determination of S. typhimurium.Exhibited linear range of 7.0 × 10(1) ∼ 7.0 × 10(9) CFU/mL, along with a detection limit as low as 23 CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1848 384525912024Aptamer-mediated double strand displacement amplification with microchip electrophoresis for ultrasensitive detection of Salmonella typhimuriumAptamer (Apt)CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT59N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-mediated double SDA-MCE method for ultrasensitive detection of S. typhimurium.Achieved a linear range of 30–1.0 × 10(5) CFU/mL and the limit of detection for S. typhimurium down to 6 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1871 386698462024Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensorsS. enterica aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-functionalized CNT-FET biosensor for the detection of these foodborne pathogens.Could detect S. enterica at a limit of detection (LOD) as low as 1 CFU in PBS buffer with a linear range from 62.5–6.4 × 10(4) CFU/mL.N/AFlow Cytometry8.9 ± 2.0 nMBiosensorN/A5'-Thiolated (HS-(CH2)6), 5'-FAM Labeled and 3'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1906 409479252025Smartphone-Based Fluorescence/Colorimetric Dual-Mode Aptasensor for the Detection of Salmonella Using Multivalent Aptamer and CHA AmplificationAptamerGTGAGAGTGGGTCATCGAAAAAACTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTCTGATGTCGGTAGT82N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a dual-mode portable fluorescent/colorimetric aptasensor, leveraging multivalent aptamer competitive assays and catalytic hairpin assembly (CHA) for the detection of Salmonella.Enables the quantitative detection of Salmonella within a linear range of 10 to 10(7) CFU/mL, with detection limits of 28 CFU/mL for colorimetric detection and 10 CFU/mL for fluorescent detection.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1910 409313012025A novel fluorescent aptasensor using magnetic silica nanospheres and Ag@MOF nanocomposites for extraction and detection of Salmonella Typhimurium in eggsAptamerTATGGCGGCGTCACCCGACGGGGACTTGCATTATGACAG39N/AssDNASalmonella Typhimurium (S. Typhimurium) (PTCC 1709)Whole cellDeveloped a fluorescent platform based on an aptamer-Ag@MOF and Fe3O4@KCC‐1 probes for the detection of S. Typhimurium in eggs.Achieved a linear detection range of 7.5 to 7.5 × 10⁶ CFU/mL, a detection limit of 4.61 CFU/mL, and a total detection time of 90 min.N/AN/AN/ABiosensorN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1921 400531472025Fluorescent aptasensor for detection of Salmonella typhimurium using boric acid-functionalized terbium metal-organic framework and magnetic nanoparticlesAptamerTAGGGAAGAGAAGGACATATGATCAGGAGTCATGCTACCACTGGTGATTACGCCTCGCTCAGCTTGACTAGTACATGACCACTTGA86N/AssDNASalmonella Typhimurium (S. Typhimurium) (MTCC 3232)Whole cellA fluorescent detection platform was developed using boric acid-functionalized terbium metal–organic framework (BA-Tb-MOF) and carboxyl-modified magnetic nanoparticles (MNPs) to identify S. typhimurium.Showed an inverse linear relationship within the range of 10(1)–10(9) CFU/mL, and the detection limit was 4 CFU/mL.N/AN/AN/ABiosensorWhole Cell-SELEX5'-FAM Labeled and 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1931 399618262025Self-protective DNAzyme-based dual-responsive three-way Y-probe for simultaneous determination of multiple pathogenic bacteriaAPT(S)CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT59N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 1925)Whole cellDeveloped a DNAzyme-based self-protecting dual-response three-way Y nanoprobe (SD-DTY) for the simultaneous detection of two foodborne pathogens.The limit of detection was 3.2 cfu/mL, and the linear range was 10-10(5) cfu/mL, with a 2-hour response time.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_2109 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA10N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.N/A15Fluorescence Binding Assay28 ± 4 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_2110 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA7N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.EA7 binds to the lipid A region of LPS and exhibits a detection limit of 3 ng/mL and a linear range of 5–250 ng/mL.15Fluorescence Binding Assay102 ± 17 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_2111 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA5N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.N/A15Fluorescence Binding Assay149 ± 30 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_2119 302453242018Aptamer functionalized MoS2-rGO nanocomposite based biosensor for the detection of Vi antigenAnti-Vi aptamers populationN/AN/A5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNASalmonella TyphiVi polysaccharide antigen (Vi antigen)Developed a novel aptamer functionalized MoS2-rGO-based electrochemical aptasensor for Vi polysaccharide antigen-mediated detection of enteric fever.Responded linearly in the range between 0.1 ng/mL to 1000 ng/mL with a detection limit of 100 pg/mL.6Saturation Binding Assay638.6 nMBiosensorSELEX5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_2120 https://doi.org/10.1016/j.jelechem.2018.07.0022018Target-induced aptamer displacement on gold nanoparticles and rolling circle amplification for ultrasensitive live Salmonella typhimurium electrochemical biosensingS. typhimurium (STM)-binding aptamer.N/AN/AN/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop an aptasensor for ultrasensitive detection of live S. typhimurium by combining target-induced aptamer displacement on gold nanoparticles (AuNPs) deposited electrode with rolling circle amplification (RCA).Showed a LOD of 16 CFU/mL with a wide linear detection range of 20 to 2 × 10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AWhen the sensor was stored at 4°C for 10 days, the electrical signal response was 90% of that obtained at the time of preparation.N/AN/AN/A
ABdb_2129 357654982022Single-stranded DNA aptamer-based rolling circle amplification as anti-chicken Salmonella bacteriostaticAnti-Salmonella DNA aptamerN/AN/AN/AssDNASalmonella Typhimurium (S. Typhimurium) and Salmonella Enteritidis (S. Enteritidis)Whole cellIdentify an aptamer targeting Salmonella in the form of RCA-p that could inhibit bacterial growth, multiplication, and viability.Significant reduction in bacterial viability.N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A