Target Organism : Mycobacterium Bovis

Total records found: 3

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_0753	27529508	2016	A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against Tuberculosis	BM2	GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CCCCATGAACTAGGCTCCACAATGAGTTTGG-GGGTCAATGCGTCATA	89	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Bovis (bacillus Calmette–Guérin (BCG))	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.	Ki of BM2 was estimated to be 10.34 ± 0.27 nM, with a LOD of 1 × 10^4 cfu/100 μL of BCG.	10	Enzyme-Linked Oligonucleotide Assay (ELONA)	8.59 ± 1.23 nM	Therapeutics/Immunmodulatory	SELEX	Biotinylated	BM2 was demonstrated to be safe with low toxicity based on in vitro and in vivo toxicity analyses.	BM2–BCG complex could be detected and remained stable for at least 30 days in vivo.	N/A	N/A	N/A
ABdb_0754	27529508	2016	A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against Tuberculosis	BM1	GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CGGTCGTAGACCGGGAGTTGTTGTTAGTTCA-GGGTCAATGCGTCATA	89	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Bovis (bacillus Calmette–Guérin (BCG))	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.	N/A	10	Enzyme-Linked Oligonucleotide Assay (ELONA)	N/A	Therapeutics/Immunmodulatory	SELEX	Biotinylated	N/A	N/A	Best Candidate	N/A	N/A
ABdb_0755	27529508	2016	A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against Tuberculosis	BM4	GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CGCGCAGCGGGTCGACTTCATTCCTCACCAT-GGGTCAATGCGTCATA	89	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Bovis (bacillus Calmette–Guérin (BCG))	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.	N/A	10	Enzyme-Linked Oligonucleotide Assay (ELONA)	N/A	Therapeutics/Immunmodulatory	SELEX	Biotinylated	N/A	N/A	N/A	N/A	N/A