AptBacterialDB

Target : Toxin A

Total records found: 41

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0263 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA1AGCAGCACAGAGGTCAGATG-AGGCGATTACGCTTCTTGTACTTCAATAACGACTCAACTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy92.17 ± 16.75 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0264 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA15/40AGCAGCACAGAGGTCAGATG-TACTTATGCATTTCCTCCCACGATCTTATTTGAGAGTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.Limit of detection of 8.7±10(-3) μg/mL with linear range of 0.01-10 μg/mL SEA.12Fluorescence Spectroscopy48.57 ± 6.52 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0265 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.2AGCAGCACAGAGGTCAGATG-ATGATCGTAGTCATTTAAAATTTGAATACTATCAAAGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy235.00 ± 79.05 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0266 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA3AGCAGCACAGAGGTCAGATG-TACGAGCGGTGGTTTTACCCTGCAATACTTTTGGCTGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0267 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA25AGCAGCACAGAGGTCAGATG-ATAGATTTTTTTCATGATTCTTCATTTTTTTATTTGAAAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0268 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA29AGCAGCACAGAGGTCAGATG-TATTATCTTACTACGTTGCATCCTACTTTTATAGTCCCCT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0269 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA30AGCAGCACAGAGGTCAGATG-GGATCTTCCCTCAATGTTTATTGTATATCTGTACTCGTAA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0270 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA10AGCAGCACAGAGGTCAGATG-TCCAATTCAATTCATTTCTGAACTTAGTCGGCACTTTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0271 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA4AGCAGCACAGAGGTCAGATG-CTCCAGCAGCCTCATAGATACGTCGTATCTATTGTTTCCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0272 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.1AGCAGCACAGAGGTCAGATG-AAGGTCTTACATCAATTTATATATTTTTCCAATATCTGTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0499 242874072014Aptamer biosensor for sensitive detection of toxin A of Clostridium difficile using gold nanoparticles synthesized by Bacillus stearothermophilusAptamerTAGTGATGCCTTTGTTGAGA-AGACTTGGGGGCAGGGTCGTGAGGACGTACGTGCGAGTGT-TCTTCATCGTCCACTAAATT805'-TAGTGATGCCTTTGTTGAGA-N40-TCTTCATCGTCCACTAAATT-3'ssDNAClostridium DifficileToxin A (TOA)Developed an electrochemical biosensor to detect TOA based on Nafion-thionine-GNPS-modified screen-printed electrode (SPE).Shows a linear range of 0–200 ng/mL with the detection limit of 1 nM for TOA.N/AN/AN/ABiosensorN/A5'-FITC LabeledN/A87.6% of its original response remaining after storage for 2 weeks at 4°C in PBS (pH 7.0).N/AN/AN/A
ABdb_0656 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.33TGTACCGTCTGAGCGATTCGTAC-CATAGGGTGCTTTTCAAGGCCACACGTTAGTGTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.100 nM or 6.6 μg/mL of Exotoxin A in human serum was detected.14Surface Plasmon Resonance (SPR)4.2 and 4.5 μMDiagnosticDecoy-SELEX5'-Amidation (NH₂-(CH2)6) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0657 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.02TGTACCGTCTGAGCGATTCGTAC-TACGCCACACGTGGTGAGGGATTCGATCGCTTGA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0658 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.20TGTACCGTCTGAGCGATTCGTAC-TGCTATTCATCACCACTCTAGAGCCACTTTTAAA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0659 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.22TGTACCGTCTGAGCGATTCGTAC-TGGGCGGCGAGCCACCCGGCAATTTAGTACAGGC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0660 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.36TGTACCGTCTGAGCGATTCGTAC-AAAGCATCCAGCCGGTATGTGCCAGAGTCTCTGA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0661 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.38TGTACCGTCTGAGCGATTCGTAC-AAATGTGAGTGGCCAGGCATCAGGTACGTCGGTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0662 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.03TGTACCGTCTGAGCGATTCGTAC-GGATAGGTGCCTCTGCTTCATCATGTTGAACTTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0663 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.13TGTACCGTCTGAGCGATTCGTAC-AGTTTCACCAGTCGCCTGTTAGCCGTGATATACG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0664 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.27TGTACCGTCTGAGCGATTCGTAC-TGTCAATATTACGTTGCTCTTAGGTTCACCATCT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0665 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.28TGTACCGTCTGAGCGATTCGTAC-TTGTGATTCAAATAGGCGTGTTGGTGTGAGACCT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0666 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.23TGTACCGTCTGAGCGATTCGTAC-CGTGGATCATGCTTCGCGTCGGTTTATAGGTTCC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0667 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.39TGTACCGTCTGAGCGATTCGTAC-AGAAGAGCATCGGTAACTTCCATAGGAGATGGGG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0668 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.06TGTACCGTCTGAGCGATTCGTAC-CATCCGAGGGTATTGTATGCGTATATCCTAGTCG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0669 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.10TGTACCGTCTGAGCGATTCGTAC-CAAGTTCCTCATGGAGGGTGCTCAGAGCTTAGAC-AGCCAGTCAGTGTTAAGGAGTAA805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0670 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.34TGTACCGTCTGAGCGATTCGTAC-AGGGGGATTCCTAGGGCCCGGCCCAACGCTGTTT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0671 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.42TGTACCGTCTGAGCGATTCGTAC-CAAGACCCTTGAATCACGGGTAGGGTCTCGTAAC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0756 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS3ATACCAGCTTATTCAATT-CCCGCCTCTGAGCATTATTAATGTTATACCTTACGGCTGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.Inhibited PBMCs proliferation with an inhibition rate of 84% and reduced IFN-γ, TNF-α, IL-2, and IL-6 secretions by 89%, 87%, 73%, 69%, respectively. PEGS3 allowed 79% of the mice to survive the lethal challenge.9Enzyme-Linked Aptamer Assay (ELAA)36.93 ± 7.29 nMTherapeutics/ImmunmodulatorySELEX5′-Polyethylene glycol (PEG) with 5'-Amidation (NH₂) and 3’-Inverted Thymidine (3'-idT) and 5'-Biotinylated or 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0757 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS6ATACCAGCTTATTCAATT-GCCGCGGAATTGTGGCTTGGTGTCGTGGTCAGGGGCTGG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.N/A9Enzyme-Linked Aptamer Assay (ELAA)N/ATherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0758 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS12ATACCAGCTTATTCAATT-GCCACTGTGGACTTGTTATTCGTCTTTGCCGTCTAATTCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.Inhibited PBMCs proliferation with an inhibition rate of 84%.9Enzyme-Linked Aptamer Assay (ELAA)79.35 ± 12.09 nMTherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0759 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS23ATACCAGCTTATTCAATT-GCAACAATGTTGTGTCAGCAAGTTGGTTGGCGGTGTATTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.N/A9Enzyme-Linked Aptamer Assay (ELAA)N/ATherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1183 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC5CCTAACCGATATCACACTCAC-GGCTAGCGGACAGGTGTAGGTCGGAATCCTTCAGCGTAGA-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1184 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC7CCTAACCGATATCACACTCAC-AGTATACCGCTCCACCAGTGTGATATCGGGATCTGCTGAC-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1185 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC10CCTAACCGATATCACACTCAC-GTAGTGAGGTGGGTCGTGAAGGGAGGCACTACTGGTGCGT-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1186 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC13CCTAACCGATATCACACTCAC-GAGGTGAGGGTGCGAGGGTAAGGGGGCAAACCTGGTGCGT-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1187 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC16CCTAACCGATATCACACTCAC-ACGTAGTGAGGGTTGCGAGGGTAAGGTGGTCAGTATACGC-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1971 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4936–4N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay3.1 nMDetectionSELEX5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1972 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4939–280N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay1.6 nMDetectionSELEX5-naphthylmethylaminocarbonyl-dU (NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1973 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4943–51N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.8Equilibrium Binding Assay1.3 nMDetectionSELEX5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1974 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5564–89N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay9.8 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1975 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5570–54N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay6.6 nMDetectionSELEX5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A