AptBacterialDB

Target : Staphylococcus aureus Protein A (SpA)

Total records found: 36

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0552 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12UGUAAUUCUGCCAUUCUUUUUGGGGCGGAAUACAGGAUGU405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.fmA12-functionalized AgNPs resulted in specific S. aureus antimicrobial activity with only a 1.6 ± 1.0% survival of 12598 cells and 51.9 ± 2.0% survival of 10832 cells.8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)95 nM (by SPR) and 67.1 ± 6.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/A76% fmA12 survives in 10% mouse serum at 37°C over 24 h, and in 50% serum, 85.0% fmA12 survives after 5 h.N/A~52 h for fmA12.N/A
ABdb_0553 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmF07AUACGCUGCUCACUAAAGUCGGGAGUACUCCAAUGUUUUC405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)38.9 ± 4.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/ABest CandidateN/AN/A
ABdb_0554 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmE09AUCUAGCAAGCACUUGCAAACAUUCGAUAACGGGGAUUC395'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)418 ± 112 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0555 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmG12UGCCAAUGUAACUCAACACUCUUCAUUGAGCUGUCGGGCA405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)475 nM (by SPR) and 220 ± 24 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0556 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ3N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.7 ± 3.5 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0557 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ6N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)63.1 ± 7.3 μMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0558 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ9N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.1 ± 16.7 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0574 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/8ATACCAGCTTATTCAATT-AGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.PA#2/8 competes with IgG or IgG-Fc for binding to Protein A.11Bead-based Binding Assay1.06 ±0.2 μMDetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0575 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/22ATACCAGCTTATTCAATT-GCAGTACTGATGAGTGTAGCCGTATGATTATCGTTTGTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0576 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/34ATACCAGCTTATTCAATT-CCCCAACGAGTCGATATGTAGCCCACACTCTGATTCGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0577 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/11ATACCAGCTTATTCAATT-GGAGACGACAAACTATTACGTACTACGGCATGCACTTGGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0578 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/3ATACCAGCTTATTCAATT-CGACAAGTGGGCATTACGATTCTAGCCCTGATTATGTTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0579 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/6ATACCAGCTTATTCAATT-ACCGATCACTAGCCGACTAATTGGTTTCCGATCGCAGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0580 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#14/82ATACCAGCTTATTCAATT-CCACAACCGAACTCGTAAGACGTATGTAGCCGCCAACTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0581 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/52ATACCAGCTTATTCAATT-ACGCATTGGAGCCCGAAACTGATTCATTGAGCCTACCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0582 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/14ATACCAGCTTATTCAATT-ACGACCGTAGACGACTTACACTGATGTTGCGCATTTCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0583 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/31ATACCAGCTTATTCAATT-CGATGACGACTGTAGCCGCAATACGCCCCTGTTACGTTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0584 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/41ATACCAGCTTATTCAATT-GGACGCCGACTAACTTTACGTGGTTCTCCTACCGCCTAACC-ACAATCGTAATCAGTTAG775'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0585 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/43ATACCAGCTTATTCAATT-ACGAAATGTAGCCGATCCTGATTACTCTCTGTCAGCTTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0784 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8ATACCAGCTTATTCAATT-AGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.PA#2/8 and its truncated variant PA#2/8[S1-58] were able to differentiate between the highly Protein A-producing S. aureus Cowan strain.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)101.4 ± 5.9 nM (of 3′-biotinylated)  and 189.9 ± 13.0 nM (of 5′-biotinylated)DetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0785 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT585'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.PA#2/8 and its truncated variant PA#2/8[S1-58] were able to differentiate between the highly Protein A-producing S. aureus Cowan strain.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)11.3 ± 1.4 nM (of 3′-biotinylated)  and 23.7 ± 2.0 nM (of 5′-biotinylated)DetectionN/A5'-and 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0786 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-50]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTC505'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0787 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-43]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGG435'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0788 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S28-50]AGGGGGATAGAGGGGGTGGGTTC235'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0953 291608512017Development of An Impedimetric Aptasensor for the Detection of Staphylococcus aureusPA#2/8[S1-58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) (DSM 20231)Staphylococcus aureus Protein A (SpA)Developed an impedimetric aptasensor for the detection of S. aureus.Showed a limit of detection of 10 CFU/mL within 10 minutes.N/AElectrochemical impedance spectroscopy (EIS)18.5 ± 1.8BiosensorN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1381 322893692020Accurate MRSA identification through dual-functional aptamer and CRISPR-Cas12a assisted rolling circle amplificationApt AATATACACCCCACCTCGCTCCCGTGACACTAATGCTATTTTTT43N/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA) (ATCC 17802)Staphylococcus aureus Protein A (SpA)Dual-functional technology based on aptamer and CRISPR-Cas12a-assisted RCA for detection of MRSA.Fluorescence intensity shows a linear correlation with the logarithm of MRSA concentration, ranging from 10(6) to 10(2) CFU/mL, spanning six orders of magnitude.N/AN/AN/ADetectionN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1590 338949562021A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognitionE. coli-aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 35150)Staphylococcus aureus Protein A (SpA)Developed a SERS aptasensor using aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) and PA modified-SERS tags (Au@DTNB@PA) for the detection of bacteria.The limit of detection (LOD) was 10 cells/mL for E. coli.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1591 338949562021A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognitionL. mono-aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNAListeria MonocytogenesStaphylococcus aureus Protein A (SpA)Developed a SERS aptasensor using aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) and PA modified-SERS tags (Au@DTNB@PA) for the detection of bacteria.The limit of detection (LOD) was 10 cells/mL for L. mono.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1592 338949562021A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognitionS. typhi-aptamerATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium)Staphylococcus aureus Protein A (SpA)Developed a SERS aptasensor using aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) and PA modified-SERS tags (Au@DTNB@PA) for the detection of bacteria.The limit of detection (LOD) for S. typhi was 25 cells/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1639 https:doi.org10.1016j.arabjc.2022.1042742022Application of G-quadruplex aptamer conjugated MSNs to deliver ampicillin for suppressing S. aureus biofilm on mice bonePA763’-ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCTACAATCGTAATCAGTTAG-5’76N/AssDNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)MSNs-APT-AMP nanosystem for antibiofilm activity against S. aureus biofilm.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1640 https:doi.org10.1016j.arabjc.2022.1042742022Application of G-quadruplex aptamer conjugated MSNs to deliver ampicillin for suppressing S. aureus biofilm on mice bonePA633’-ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCTACAAT-5’63N/AssDNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)MSNs-APT-AMP nanosystem for antibiofilm activity against S. aureus biofilm.No significant biofilm on the surface of the bone after 48 h treatment with 100 µg/mL of the three-component system.N/AN/AN/ATherapeuticsN/AN/ANo significant toxicity at 100 µg/mL for MCF-7 cells in 48 h.N/ABest CandidateN/AN/A
ABdb_1641 https:doi.org10.1016j.snb.2022.1327522022Novel nanozyme-catalyzed and magnetically assisted colorimetric biosensor for Staphylococcus aureus detection with a low matrix effect from complex environmentsSA31GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Staphylococcus aureus Protein A (SpA)Vancomycin-modified magnetic nanoparticles (Fe3O4 @Au-Van MNPs) combined with an aptamer were prepared for S. aureus detection.The biosensor can distinguish more than 100 CFU/mL of pathogens by the naked eye and LOD of 2 CFU/mL for S. aureus.N/AN/AN/ABiosensorN/AFAM LabeledN/AN/AN/AN/AN/A
ABdb_1841 397279012024Competitive Electrochemical Apta-Assay Based on cDNA-Ferrocene and MXenes for Staphylococcus aureus Surface Protein A DetectionAPTATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Staphylococcus aureus Protein A (SpA)Developed a competitive aptasensor based on a ferrocene (Fc)-labeled cDNA hybridized (cDNA-Fc S13) on a specific aptamer (APT) for PrA in the presence of MXene nanosheets for the indirect detection of S. aureus.Displayed a linear range of 10–125 nM, an LOD of 3.33 nM, and a response time under 40 min.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1849 https://doi.org/10.1016/j.biosx.2023.1004362024DNA origami-enhanced binding of aptamers to Staphylococcus aureus cellsPA#2/8 [1–58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) SA5Staphylococcus aureus Protein A (SpA)Developed a DNA origami-based biosensor for the detection of SA.Five-times higher affinity of the aptamer-functionalized DNA origami compared to pure aptamers.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)160 ± 9 nMBiosensorN/A5'-PolyA (21A) or PolyT (21 T)N/AN/AN/AN/AN/A
ABdb_1988 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4520–8PCGGCPPCGGGPACCPAPPAPCGGPPPAGCCCAGPCAGAA40N/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Staphylococcus aureus Protein A (SpA)Developed slow off-rate modified aptamer (SOMAmer) reagents against S. aureus cell surface-associated proteins, used to capture and detect S. aureus.Efficient capture of Staph. aureus over a wide range of cell densities from 5 × 10(2) CFU/ml to 5 × 10(9) CFU/ml.8Radiolabel Filter Binding Assay0·22 nMDetectionSELEXP=NapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1990 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4531–56N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Staphylococcus aureus Protein A (SpA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.Able to bind whole cells of all Staph. aureus strains tested, with a detection limit of approx. 10(4) cells per well (10(5)-10(6) cells/ml).8Radiolabel Filter Binding Assay0·03 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A