Target : Outer membrane protein Ag1 (E. coli OMP Ag1)

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_1499 322008872020DNA aptamer-based non-faradaic impedance biosensor for detecting E. coliOMP Ag1 aptamerATCCGTCACACCTGCTCT-ACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA-TGGTGTTGGCTCCCGTAT73N/AssDNAEscherichia Coli (E. Coli) BL21 strainOuter membrane protein Ag1 (E. coli OMP Ag1)Developed an impedance-based biosensor using aptamer-functionalized Interdigitated Electrode (IDE) arrays to detect E. coli.Detection limit of 9 cfu/mL and linear concentration range of 25–1000 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1853 https://doi.org/10.1016/j.microc.2024.1114282024Colorimetric and fluorescent dual-mode detection of Escherichia coli using Fe3O4 nanoparticle coated with fluorescein-labeled aptamerE. coli aptamerATCCGTCACACCTGCTCT-ACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA-TGGTGTTGGCTCCCGTAT73N/AssDNAEscherichia Coli (E. Coli) BL21 strainOuter membrane protein Ag1 (E. coli OMP Ag1)Developed a dual-mode colorimetric and fluorescence assay employing Fe3O4 nanoparticles (NPs) coated with FAM-Apt for the identification of E. coli.Acheived an impressive limit of detection (LOD) of 10 CFU/mL in the colorimetric mode and 6 CFU/mL in the fluorescence mode.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A