AptBacterialDB

Target : Mannose-capped lipoarabinomannan (ManLAM) epitope

Total records found: 24

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0343 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL1GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GGCGCCATAGCGACGGGGCCATTCCAAGAA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).ZXL1 bound to virulent M. tb H37Rv much more strongly (52.2%) than to the other Mycobacteria strains tested, M. smegmatis (2.5%) and BCG (13.9%). LPS-stimulated human DC groups showed that ZXL1 decreased IL-10 production by 31–36% and increased IL-12 production by 52–91%, thereby reducing the progression of infection and bacterial loads in mice and rhesus monkeys.12Enzyme-Linked Oligonucleotide Assay (ELONA)436.3 ± 37.84 nMTherapeutics/ImmunmodulatorySELEXBiotinylated or FAM LabeledZXL1 showed no significant cytotoxicity at concentrations ranging from 2 to 15 microM at the 168-hour test.N/ABest CandidateN/AN/A
ABdb_0344 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL2GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-AGTCCACGATAATCACACACACGGACACCC-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0345 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL4GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CACGCCCAATAGTGGCTCCAGCTCATCTCT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0346 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL5GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CATCGATGTACCCTACCTACATGTTACGTT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0347 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL6GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-ACCTACAGCGACACCTAACTGAGTAGAACT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0348 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL7GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TACCTAACACTTCCTGCTCCACCTTTATTA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0349 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL8GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CCCTGATTCCCCCTCATCCGATGGACATCT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0350 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL9GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GACCCTCATGATACCAACCACTCCAGTCAA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0351 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL10GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TATCCCAGTGGCATTAACTACCCACTCGCA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0352 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL3GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TCACGGAGTGAGGGGCTGTTCATTAAGAGA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0353 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL12GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TCGCCTGATAGGTCTGTGATCCAATCCAAT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0354 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL13GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TCTGCTTGCCGCTGATCCGCCAATTCGATC-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0355 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL14GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TCGTGCCGACGCTGCCGAATAGCACTGCAC-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0753 275295082016A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against TuberculosisBM2GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CCCCATGAACTAGGCTCCACAATGAGTTTGG-GGGTCAATGCGTCATA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Bovis (bacillus Calmette–Guérin (BCG))Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.Ki of BM2 was estimated to be 10.34 ± 0.27 nM, with a LOD of 1 × 10^4 cfu/100 μL of BCG.10Enzyme-Linked Oligonucleotide Assay (ELONA)8.59 ± 1.23 nMTherapeutics/ImmunmodulatorySELEXBiotinylatedBM2 was demonstrated to be safe with low toxicity based on in vitro and in vivo toxicity analyses.BM2–BCG complex could be detected and remained stable for at least 30 days in vivo.N/AN/AN/A
ABdb_0754 275295082016A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against TuberculosisBM1GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CGGTCGTAGACCGGGAGTTGTTGTTAGTTCA-GGGTCAATGCGTCATA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Bovis (bacillus Calmette–Guérin (BCG))Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.N/A10Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0755 275295082016A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against TuberculosisBM4GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CGCGCAGCGGGTCGACTTCATTCCTCACCAT-GGGTCAATGCGTCATA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Bovis (bacillus Calmette–Guérin (BCG))Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.N/A10Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0832 282066802017A single-stranded DNA aptamer against mannose-capped lipoarabinomannan enhances anti-tuberculosis activity of macrophages through downregulation of lipid-sensing nuclear receptor peroxisome proliferator-activated receptor γ expressionZXL1GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GGCGCCATAGCGACGGGGCCATTCCAAGAA-GGGTCAATGCGTCATA88N/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeDownregulate PPARγ expression by blocking the binding of ManLAM to MR on macrophages, and ZXL1 enhances IL-1β and IL-12 mRNA expression and cytokine production in ManLAM-treated macrophages but decreases IL-10 production.The percentage of macrophages that took up iH37Rv decreased from 15.9% to 7.2%, thereby inhibiting ManLAM-induced immunosuppression of DCs.N/AN/AN/ATherapeutics/ImmunmodulatoryN/ABiotinylatedN/AN/AN/AN/AN/A
ABdb_0966 https://doi.org/10.1016/j.snb.2017.07.0742017A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphoneDirect Dot-Blot AptamerGGCGCCATAGCGACGGGGCCATTCCAAGAA30N/AssDNAMycobacterium Tuberculosis (H37Ra)Mannose-capped lipoarabinomannan (ManLAM) epitopeSmartphone-based method using enzyme-linked aptamers for point-of-need M.tb detection.The direct dot-blot system provided a lower limit of quantitation of 10(4) CFU/mL and greater accuracy.N/AN/AN/ADetectionN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0967 https://doi.org/10.1016/j.snb.2017.07.0742017A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphoneIndirect Dot-Blot AptamerGCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GGCGCCATAGCGACGGGGCCATTCCAAGAA-GGGTCAATGCGTCATA88N/AssDNAMycobacterium Tuberculosis (H37Ra)Mannose-capped lipoarabinomannan (ManLAM) epitopeSmartphone-based method using enzyme-linked aptamers for point-of-need M.tb detection.The limit of quantitation (LOQ) for indirect assays was 10(5) CFU/mL.N/AN/AN/ADetectionN/AN/AN/AN/AN/AN/AN/A
ABdb_2018 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT9N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.Specificity and sensitivity were 95.31% and 83.00% (for 100 aPTB serum samples), 98.70% and 92.71% (for 96 aPTB sputum samples), and 94.44% and 88.71% (for 62 EPTB serum samples), respectively.13Enzyme-Linked Oligonucleotide Assay (ELONA)668 ± 159 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_2019 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT25N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.N/A13Enzyme-Linked Oligonucleotide Assay (ELONA)685 ± 131 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/AN/AN/AN/A
ABdb_2020 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT14N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.N/A13Enzyme-Linked Oligonucleotide Assay (ELONA)815 ± 144 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/AN/AN/AN/A
ABdb_2021 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT15N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.N/A13Enzyme-Linked Oligonucleotide Assay (ELONA)736 ± 207 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/AN/AN/AN/A
ABdb_2022 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT6N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.N/A13Enzyme-Linked Oligonucleotide Assay (ELONA)998 ± 207 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/AN/AN/AN/A