AptBacterialDB

Target : MPT64 protein

Total records found: 17

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0065 192842972009The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosisM64CA (aptamers 20, 34, 41, 46, and 50 )GGGAGCTCAGAATAAACGCTCAA-TTCGGGAATGATTATCAAATTTATGCCCTCTGAT-TTCGACATGAGGCCCGGATC775'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinIdentify an aptamer that binds to MPT64, and an aptamer-MPT64 was designed to detect M. tuberculosis by measuring MPT64 protein levels in culture filtrates.The sandwich assay scheme based on the aptamer-protein complex had a high sensitivity (86.3%) and specificity (88.5%).12N/AN/ADetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0066 192842972009The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosisAptamer 11 (named M64RA)GGGAGCTCAGAATAAACGCTCAA-TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinIdentify an aptamer that binds to MPT64, and an aptamer-MPT64 was designed to detect M. tuberculosis by measuring MPT64 protein levels in culture filtrates.The sandwich assay scheme based on the aptamer-protein complex had a high sensitivity (86.3%) and specificity (88.5%).12N/AN/ADetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0933 284546522017Selection, characterization, and application of DNA aptamers for detection of Mycobacterium tuberculosis secreted protein MPT64Aptamer 17TCACTTCAAATGTGCGCTTC-GTCAACAGTCCAGTTGGGAGGTCGCTTATAGCTGCTTGTC-CGTCAAAACAGGGGGTAGAA805'-TCACTTCAAATGTGCGCTTC-N40-CGTCAAAACAGGGGGTAGAA-3'ssDNAMycobacterium Tuberculosis (H37Rv)MPT64 protein (Rv1980c)Identify DNA aptamers against MPT64 protein.The ELONA assay had a sensitivity and specificity of 91.3% and 90% on sputum samples.10Surface Plasmon Resonance (SPR)8.92 nMDetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0947 285511172017Electrochemical determination of M. tuberculosis antigen based on Poly(3,4-ethylenedioxythiophene) and functionalized carbon nanotubes hybrid platformMPT64 aptamerGTACAAACGACGGCCAGT-CCTTGGGATGATTCAAGCAAAGCCTCACGCCTACGGCTAA-GTCATAGCTGTCTCTCCTG775'-GTACAAACGACGGCCAGT-N40-GTCATAGCTGTCTCTCCTG-3'ssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDeveloped an electrochemical aptasensor based on MWCNT-doped PEDOT nano-composite for the detection of Mycobacterium tuberculosis.Exhibits a LOD of 0.5 fg/mL within 15 min for the detection of M. tb antigenic protein with recovery in between (88–95)%.N/AN/AN/ABiosensorSELEX5'-BiotinylatedN/AThe stability of the aptasensor is 27 days at 4°C, and the reusability is 7 times after repeated regeneration with 50 mM NaOH.N/AN/AN/A
ABdb_0948 284149752017Fullerene-doped polyaniline as new redox nanoprobe and catalyst in electrochemical aptasensor for ultrasensitive detection of Mycobacterium tuberculosis MPT64 antigen in human serumMBA ITGGGAGCTGATGTCGCATGGGTTTTGATCACATGA35N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinElectrochemical aptasensor based on GNPs-C60-PAn labelled with signal aptamer for the determination of MTB antigen.A wide linear detection range of 0.02 to 1000 pg/mL was obtained, with a detection limit of 20 fg/mL for MPT64.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0949 284149752017Fullerene-doped polyaniline as new redox nanoprobe and catalyst in electrochemical aptasensor for ultrasensitive detection of Mycobacterium tuberculosis MPT64 antigen in human serumMBA IITTCGGGAATGATTATCAAATTTATGCCCTCTGAT34N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinElectrochemical aptasensor based on GNPs-C60-PAn labelled with signal aptamer for the determination of MTB antigen.A wide linear detection range of 0.02 to 1000 pg/mL was obtained, with a detection limit of 20 fg/mL for MPT64.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0979 https://doi.org/10.1007/s00604-017-2174-72017Aptamer based voltammetric biosensor for the detection of Mycobacterium tuberculosis antigen MPT64Aptamer 3GTACAAACGACGGCCAGT-CCTTGGGATGATTCAAGCAAAGCCTCACGCCTACGGCTAA-GTCATAGCTGTCTCTCCTG775'-GTACAAACGACGGCCAGT-N40-GTCATAGCTGTCTCTCCTG-3'ssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDevelop an aptaelectrode based on graphene modified iron-oxide chitosan hybrid (CHIT-IO-GR) nanocomposite film deposited on fluorine tin oxide (FTO) for the detection of the M. tb. specific antigen MPT64.Exhibited a limit of detection (LOD) of 0.9 fg/mL within 20 min and a linear range from 1.0 × 10(5) fg/mL to 1.0 fg/mL.10N/AN/ABiosensorSELEX5'-BiotinylatedN/AThe biosensor retained about 80% of its initial activity after 10 uses and good stability of 17 day.Best CandidateN/AN/A
ABdb_1191 304210382018Dual-aptamer-based voltammetric biosensor for the Mycobacterium tuberculosis antigen MPT64 by using a gold electrode modified with a peroxidase loaded composite consisting of gold nanoparticles and a Zr(IV)/terephthalate metal-organic frameworkAPT-ITGGGAGCTGATGTCGCATGGGTTTTGATCACATGA35N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinA sandwich-like dual-aptamer strategy employing an ultrasensitive aptasensor for the voltammetric determination of M. tuberculosis. antigen MPT64 in human serum.Achieved an ultrasensitive detection limit of 10 fg/mL and a wide linear response range from 0.02 to 1000 pg/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1192 304210382018Dual-aptamer-based voltammetric biosensor for the Mycobacterium tuberculosis antigen MPT64 by using a gold electrode modified with a peroxidase loaded composite consisting of gold nanoparticles and a Zr(IV)/terephthalate metal-organic frameworkAPT-IITTCGGGAATGATTATCAAATTTATGCCCTCTGAT34N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinA sandwich-like dual-aptamer strategy where two different aptamers are immobilized on a gold electrode to synergistically bind the target MPT64 proteinAchieved an ultrasensitive detection limit of 10 fg/mL and a wide linear response range from 0.02 to 1000 pg/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1325 312021032019A sandwich-type electrochemical aptasensor for Mycobacterium tuberculosis MPT64 antigen detection using C60NPs decorated N-CNTs/GO nanocomposite coupled with conductive PEI-functionalized metal-organic frameworkMPT64 antigen aptamer Ⅰ (MAA Ⅰ)TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA35N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDevelop a sandwich-type electrochemical aptasensor using (C60NPs-N-CNTs/GO) for the detection of MPT64 antigen.Showed a wide linear range from 1 fg/mL to 1 ng/mL with a limit of detection (LOD) as low as 0.33 fg/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AOxidation current remains 94.5% of the initial level after 80 cycles, and peak current remains approximately 98.7%, 93.5%, and 90.6% of the initial peak current after 7, 14, and 21 days, respectively.N/AN/AN/A
ABdb_1326 312021032019A sandwich-type electrochemical aptasensor for Mycobacterium tuberculosis MPT64 antigen detection using C60NPs decorated N-CNTs/GO nanocomposite coupled with conductive PEI-functionalized metal-organic frameworkMPT64 antigen aptamer Ⅱ (MAA Ⅱ)TTCGGGAATGATTATCAAATTTATGCCCTCTGAT34N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDevelop a sandwich-type electrochemical aptasensor using (C60NPs-N-CNTs/GO) for the detection of MPT64 antigen.Showed a wide linear range from 1 fg/mL to 1 ng/mL with a limit of detection (LOD) as low as 0.33 fg/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AOxidation current remains 94.5% of the initial level after 80 cycles, and peak current remains approximately 98.7%, 93.5%, and 90.6% of the initial peak current after 7, 14, and 21 days, respectively.N/AN/AN/A
ABdb_1340 300786222019Electrochemical aptasensor using optimized surface chemistry for the detection of Mycobacterium tuberculosis secreted protein MPT64 in human serumAptamerTCACTTCAAATGTGCGCTTC-GTCAACAGTCCAGTTGGGAGGTCGCTTATAGCTGCTTGTC-CGTCAAAACAGGGGGTAGAA80N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDeveloped an electrochemical aptasensor using thiol PEGylated aptamer HS-(CH2)6-OP(O)2O-(CH2CH2O)6-TTTTT-aptamer and 6-mercaptohexanol for the detection of MPT64.A spiked human serum sample displays a limit of detection of 81 pM in 30 minutes with the long linker aptamer/MCH.N/AN/A8.92 nMBiosensorN/AHS-(CH2)6-OP(O)2O-(CH2CH2O)6-5'-TTTTT-aptamer-3' and HS-(CH2)6-5'-TTTTT-aptamer-3'N/AN/AN/AN/AN/A
ABdb_1713 363545052022Simultaneous Amperometric Aptasensor Based on Diazonium Grafted Screen-Printed Carbon Electrode for Detection of CFP10 and MPT64 Biomarkers for Early Tuberculosis DiagnosisMPT64 aptamerTTTTTTTTTTGGGAGCTGATGTCGCATGGGTTTTGATCACATGA44N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinAmperometric dual aptasensor for the simultaneous detection of M. tb. secreted antigens CFP10 and MPT64 for the diagnosis of TB.Achieved a detection limit of 1.82 ng/mL and a linear range of 0.75–250 ng/mL for MPT64 antigen within 2.5 h.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1893 412456392025Simultaneous and sensitive detection of Mycobacterium tuberculosis and SARS-CoV-2 antigens employing an electrochemical impedance spectroscopy aptasensorMPT64 aptamer sequence (17)GTCAACAGTCCAGTTGGGAGGTCGCTTATAGCTGCTTGTC40N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDevelop an EIS-based aptasensor fabricated to detect MPT64 and S-glycoprotein simultaneously, employing a dual-platform approach on a screen-printed gold electrode (SPGE).Achieved detection limits of 0.053 pg/mL in buffer and in human serum of 0.085 pg/mL with a linear range of 0.01 pg/mL to 10 pg/mL in both buffer and human serum.N/AN/A8.92 nMBiosensorN/A5'-Thiolated (HS(CH2)6-(CH2CH2O)6-TTTTT)N/AThe aptasensor maintained good storage stability for up to 22 days.N/AN/AN/A
ABdb_2086 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7615-18N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MPT64 protein (Rv1980c)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay12.3 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2087 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14496-43N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MPT64 protein (Rv1980c)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay2.07 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2121 317001252019An aptasensor for the detection of Mycobacterium tuberculosis secreted immunogenic protein MPT64 in clinical samples towards tuberculosis detectionAptamerN/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv)MPT64 proteinElectrochemical impedance spectroscopy (EIS) aptasenosor based on an IDE for the detection of MPT64.Display LOD of 4.1 fM and the specificity and sensitivity for the sputum sample analysis 100% and 76.47%, respectively, and for the serum sample analysis 100% and 88.24%, respectively.N/AN/A8.92 nMBiosensorN/A5'-Thiolated (HS-(CH6)6-OP(O)2O-(CH2CH2O)6-5′-TTTTT)N/AN/AN/AN/AN/A