AptBacterialDB

Target : HspX antigen

Total records found: 12

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_1003 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH63GTCTTGACTAGTTACGCC-GGGAACAATATGTTCAAGGGCTCTTTAAAGTTTTAGTTCGTTTG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.LOD was 32 ng for H63.8Isothermal Titration Calorimetry (ITC)3.71 x 10-7 ± 2.12 x 10-11 MDiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AIndian Patent application no. 201611001550
ABdb_1004 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH63 SL-2 M6AGGGCTTTTTTTTTTTTTAGTTCGTTTG285'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.Exhibited 2.5-fold higher binding with respect to the parent H63, and H63 SL-2 M6 has an LOD of 16 ng.8Isothermal Titration Calorimetry (ITC)9 x 10-8 ± 2 x 10-14 MDiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AIndian Patent application no. 201611001550
ABdb_1005 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH33GTCTTGACTAGTTACGCC-ACACTAGAAGGTATTTACTATTCTGTGTTAACATGTACTAGCCA-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1006 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH66GTCTTGACTAGTTACGCC-AAGTTTTAATACAACTCACATTAGGGAAAGCTTGCATCCAGGCC-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1007 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH70GTCTTGACTAGTTACGCC-CAGGACAGGTTAAAATTTTTCTGGATCCCTTTTCATTCTCCATGG-TCATTCAGTTGGCGCCTC815'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1008 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH3GTCTTGACTAGTTACGCC-CATACACGGTTACACGTACTGGATAATGTTGATTATGCTTCTGC-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1009 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH18GTCTTGACTAGTTACGCC-TAGAACCAAACCTTGTGTAGAAGGGCTTCTCCCGTGTGCTTTGA-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1010 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH47GTCTTGACTAGTTACGCC-GGATGAACTGTTCCGCTGTGTATCACGCCAAAAGTCCTTATTTG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1011 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH48GTCTTGACTAGTTACGCC-CATGAAACCATCTGGTCCGCAATGGGCGCTTGGAATATTCGTCA-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1093 303505642018Aptamer-Based TB Antigen Tests for the Rapid Diagnosis of Pulmonary Tuberculosis: Potential Utility in Screening for TuberculosisH63 SL-2-M6AGGGCTTTTTTTTTTTTTAGTTCGTTTG28N/AssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenDeveloped an Aptamer ALISA and an electrochemical sensor (ECS) for the direct detection of HspX in sputum.Aptamer ALISA demonstrated excellent sensitivity of 94.1% (95% CI, 86.8–98%) and specificity of 100% (95% CI, 98.4–100%). Aptamer-based ECS detected HspX at ∼13 pM.N/AN/AN/ABiosensorN/AALISA: 5′-Biotinylated and ECS: 5'-Methylene Blue (MB) and 3'-ThiolatedN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1276 303521982019A novel aptamer-based test for the rapid and accurate diagnosis of pleural tuberculosisH63 SL-2 M6AGGGCTTTTTTTTTTTTTAGTTCGTTTG28N/AssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenAptamer-Linked Immobilized Sorbent Assay (ALISA) was developed to detect pleural TB (pTB).Detects pTB with ~93% sensitivity and ~98% specificity.N/AN/AN/ADiagnosticN/A5'-BiotinylatedN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1277 309886112019Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitisH63 SL-2 M6AGGGCTTTTTTTTTTTTTAGTTCGTTTG28N/AssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenDeveloped a structural switching electrochemical aptasensor using methylene blue (MB) and an aptamer for the detection of HspX in CSF samples for the diagnosis of TBM.Can detect as low as 10 pg HspX in CSF with ~95% sensitivity and ~97.5% specificity in ≤30 minutes.N/AN/AN/ADiagnosticN/A5'-Methylene Blue (MB) and 3'-C6 thiol modificationN/AN/AN/AN/AIndian Patent application no. 201611001550