AptBacterialDB

Target : Clumping factor A (ClfA)

Total records found: 13

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_1934 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 7.1ACACTAGATCAGTCACAG-GATTAAGGATCGTTGTTGGGTGGTTTCCGTAATTACTATC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1935 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 15.1ACACTAGATCAGTCACAG-GGTTGCTCAGGAGAAGGGAACGTGGGTGGTGGCGCGGACG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1936 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 3.1ACACTAGATCAGTCACAG-ACGGGGAGAGGGTCATCTCTGGTTTCGGGGTGCTAATTCT-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1937 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 8.1ACACTAGATCAGTCACAG-CCCCGACCTGTACACGTACTACCCGATGACATTTGGTGTT-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1938 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 13.1ACACTAGATCAGTCACAG-TGGACGCGGCGGGCCGGATTAAGTTGAGGAGAGGGGGGTG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1939 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 16.1ACACTAGATCAGTCACAG-AGTATATATGGATTCCGCAACGTACGCAGGGGGTGTCGCC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1940 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 14.1ACACTAGATCAGTCACAG-CAGAGAGGGGCGGCGAGGGACAAAGGAGGGTAGGTGTGCC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1941 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 12.1ACACTAGATCAGTCACAG-ATCACAGCGGAGGTTTAACGTGTGGCGGGTGTGGGGACATG-GACTTGACTCAGACATCT775'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1942 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 11.1ACACTAGATCAGTCACAG-GAGCAACAGTCTCGCCTTCGTTACTGCGCGCGGGACGGGG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.CA 11.1 exhibited S. aureus binding in a linear range of 10(3) to 10(8) CFU/mL and a limit of detection (LOD) of 10(3) CFU/mL.11Bead-based Binding Assay263.8 ± 78 nMDetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1943 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 17.1ACACTAGATCAGTCACAG-AAGTGGAATCAAGCATGGGTATTTTAAGCATCGTATCTCG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1944 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 5.1ACACTAGATCAGTCACAG-TGTTTCACGGGGGGGAGGGTAACGGAAGCTAATGGTCTCG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1989 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4503–73AZCZGGZZCAAAGZGGCGAZZGGGCAZCZGGZZZZZAAGZ40N/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor A (ClfA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.Efficient capture of Staph. aureus over a wide range of cell densities from 5 × 10(2) CFU/ml to 5 × 10(9) CFU/ml.8Radiolabel Filter Binding Assay0·79 nMDetectionSELEXZ=BndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1991 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4522–5N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor A (ClfA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·35 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A