Target : Binary toxin, B chain (CdtB)

Total records found: 2

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_1986	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5556–51	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Binary toxin, B chain (CdtB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	6.9 nM	Detection	SELEX	5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1987	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5556–67	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Binary toxin, B chain (CdtB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	4.4 nM	Detection	SELEX	5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A