Target : Acetohydroxyacid synthase (AHAS)

Total records found: 11

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_0527	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt1	ATGCGGATCCCGCGC-CGAGTGAGGGCGAGGCGCGCTCCTGCCGGT-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	IC₅₀ of 28.94 ± 0.002 nM and showed a MIC of 5.36 μg/ml against MDR (M22, M23, and P887) and XDR (X24, X59) strains.	10	Enzyme-Linked Immunosorbent Assay (ELISA)	1.06 ± 0.10 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	N/A	N/A	N/A
ABdb_0528	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt6	ATGCGGATCCCGCGC-CGGCCAGGGGACGAGCGCGCCCTGATCGTG-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	IC₅₀of 22.35 ± 0.001 nM and showed MIC of 6.24 μg/ml against MDR (M22, M23, and P887) and XDR (X24, X59) strains.	10	Enzyme-Linked Immunosorbent Assay (ELISA)	0.210 ± 0.05 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	N/A	N/A	N/A
ABdb_0529	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Apt1_M3 (17-mer)	ATGCGGATCCCGCGC-GAGGGCGAGGCGCGCTC-GCGCAAGCTTCGCGC	47	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	Significant growth inhibition against MDR-TB and XDR-TB strains of tuberculosis with a very low MIC of 5.36 μg/ml.	10	Enzyme-Linked Immunosorbent Assay (ELISA)	4.90 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	Mtb-Apt1 (17-mer) and Mtb-Apt6 (20-mer) do not exhibit significant toxicity at the tested concentrations ranging from 0 to 20 μM.	N/A	N/A	N/A	N/A
ABdb_0530	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Apt6_M3 (20-mer)	ATGCGGATCCCGCGC-CAGGGGACGAGCGCGCCCTG-GCGCAAGCTTCGCGC	50	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	Significant growth inhibition against MDR-TB and XDR-TB strains of tuberculosis with a very low MIC of 6.24 μg/ml.	10	Enzyme-Linked Immunosorbent Assay (ELISA)	364 nM	Therapeutics	DNA-SELEX	5'-Biotinylated	Mtb-Apt1 (17-mer) and Mtb-Apt6 (20-mer) do not exhibit significant toxicity at the tested concentrations ranging from 0 to 20 μM.	N/A	Best Candidate	N/A	N/A
ABdb_0531	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt2	ATGCGGATCCCGCGC-GGCACCCAGTGTGGCGCGCCTCCCTCCGTC-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	N/A	10	Enzyme-Linked Immunosorbent Assay (ELISA)	0.286 ± 0.64 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	Best Candidate	N/A	N/A
ABdb_0532	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt3	ATGCGGATCCCGCGC-GCCCACCTGTGGGGCGCGCCTCCCTCCGTC-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	Showed moderate to lower inhibition specificities.	10	Enzyme-Linked Immunosorbent Assay (ELISA)	0.677 ± 0.14 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	N/A	N/A	N/A
ABdb_0533	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt4	ATGCGGATCCCGCGC-GCCCACGTGTGGTGCGCGCCTCCTCGTAGT-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	Showed moderate to lower inhibition specificities.	10	Enzyme-Linked Immunosorbent Assay (ELISA)	0.956 ± 0.20 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	N/A	N/A	N/A
ABdb_0534	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt5	ATGCGGATCCCGCGC-GGCACCCAGTGTGGCGCGCCTCCTCGTAGT-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	N/A	10	Enzyme-Linked Immunosorbent Assay (ELISA)	2.03 ± 0.12 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	N/A	N/A	N/A
ABdb_0535	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt7	ATGCGGATCCCGCGC-ACGCGACAGCAGTGCGCGCCCCGTCCCGGT-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	N/A	10	Enzyme-Linked Immunosorbent Assay (ELISA)	1.02 ± 0.13 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	N/A	N/A	N/A
ABdb_0536	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt8	ATGCGGATCCCGCGC-CGACGGAGGGAGGCGCGCCACACTGGGTGC-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	N/A	10	Enzyme-Linked Immunosorbent Assay (ELISA)	0.55 ± 0.05 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	N/A	N/A	N/A
ABdb_0537	25988243	2015	Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthase	Mtb-Apt9	ATGCGGATCCCGCGC-GCACCGGCAGGAGCGCGCCTCGCCCTCACT-GCGCAAGCTTCGCGC	60	5'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'	ssDNA	Mycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strains	Acetohydroxyacid synthase (AHAS)	Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.	N/A	10	Enzyme-Linked Immunosorbent Assay (ELISA)	1.66 ± 0.22 μM	Therapeutics	DNA-SELEX	5'-Biotinylated	N/A	N/A	N/A	N/A	N/A