Modification : Biotinylated or FAM Labeled

Total records found: 6

Download:

Search:

AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_0343	24572295	2014	Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeys	ZXL1	GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GGCGCCATAGCGACGGGGCCATTCCAAGAA-GGGTCAATGCGTCATA	88	5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Tuberculosis (H37Rv) (ATCC 93009)	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).	ZXL1 bound to virulent M. tb H37Rv much more strongly (52.2%) than to the other Mycobacteria strains tested, M. smegmatis (2.5%) and BCG (13.9%). LPS-stimulated human DC groups showed that ZXL1 decreased IL-10 production by 31–36% and increased IL-12 production by 52–91%, thereby reducing the progression of infection and bacterial loads in mice and rhesus monkeys.	12	Enzyme-Linked Oligonucleotide Assay (ELONA)	436.3 ± 37.84 nM	Therapeutics/Immunmodulatory	SELEX	Biotinylated or FAM Labeled	ZXL1 showed no significant cytotoxicity at concentrations ranging from 2 to 15 microM at the 168-hour test.	N/A	Best Candidate	N/A	N/A
ABdb_2018	26850356	2016	Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosis	T9	N/A	N/A	5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Tuberculosis (M.Tb.) Beijing genotype strains	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.	Specificity and sensitivity were 95.31% and 83.00% (for 100 aPTB serum samples), 98.70% and 92.71% (for 96 aPTB sputum samples), and 94.44% and 88.71% (for 62 EPTB serum samples), respectively.	13	Enzyme-Linked Oligonucleotide Assay (ELONA)	668 ± 159 nM	Detection	SELEX	Biotinylated or FAM Labeled	N/A	N/A	Best Candidate	N/A	N/A
ABdb_2019	26850356	2016	Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosis	T25	N/A	N/A	5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Tuberculosis (M.Tb.) Beijing genotype strains	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.	N/A	13	Enzyme-Linked Oligonucleotide Assay (ELONA)	685 ± 131 nM	Detection	SELEX	Biotinylated or FAM Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_2020	26850356	2016	Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosis	T14	N/A	N/A	5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Tuberculosis (M.Tb.) Beijing genotype strains	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.	N/A	13	Enzyme-Linked Oligonucleotide Assay (ELONA)	815 ± 144 nM	Detection	SELEX	Biotinylated or FAM Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_2021	26850356	2016	Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosis	T15	N/A	N/A	5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Tuberculosis (M.Tb.) Beijing genotype strains	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.	N/A	13	Enzyme-Linked Oligonucleotide Assay (ELONA)	736 ± 207 nM	Detection	SELEX	Biotinylated or FAM Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_2022	26850356	2016	Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosis	T6	N/A	N/A	5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'	ssDNA	Mycobacterium Tuberculosis (M.Tb.) Beijing genotype strains	Mannose-capped lipoarabinomannan (ManLAM) epitope	Identify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.	N/A	13	Enzyme-Linked Oligonucleotide Assay (ELONA)	998 ± 207 nM	Detection	SELEX	Biotinylated or FAM Labeled	N/A	N/A	N/A	N/A	N/A