Modification : 5'-Thiolated and 5'-FAM Labeled

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_1807	https://doi.org/10.1016/j.aac.2023.07.005	2023	DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stages	B15	AGCAGCACAGAGGTCAGATG-GGCGGGTTTGGATCTTTGGTTGGCGCCTGTTTCTTTATGA-CCTATGCGTGCTACCGTGAA	80	5'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'	ssDNA	Bacillus Cereus (CICC 10041)	Whole cell	Identify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.	Achieve highly sensitive detection of B. cereus as low as 10 cfu/mL.	16	Flow Cytometry	16.13 ± 4.98 nM	Biosensor	Whole Cell-SELEX	5'-Thiolated and 5'-FAM Labeled	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1808	https://doi.org/10.1016/j.aac.2023.07.005	2023	DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stages	B16	AGCAGCACAGAGGTCAGATG-ATATGTTTACGCCAGTGGTATTATTGGGGTTGATATGTCA-CCTATGCGTGCTACCGTGAA	80	5'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'	ssDNA	Bacillus Cereus (CICC 10041)	Whole cell	Identify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.	Achieve highly sensitive detection of B. cereus as low as 10 cfu/mL.	16	Flow Cytometry	20.67 ± 5.23 nM	Biosensor	Whole Cell-SELEX	5'-Thiolated and 5'-FAM Labeled	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1922	39905704	2025	Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micra	Apt-ss1	TGACTGACGACGACTC-CCCGCAGGGGATCCGGATGCCGCGTTGGAGGAGATATGTATTATTCCATC-GACTGCTCGAGCTG	80	5'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'	ssDNA	Parvimonas Micra (ATCC 33270)	Whole cell	Isolate aptamers against P. micra and develop colorimetric aptsensor using Au@Fe3O4 nanoparticles.	Demonstrated a linear response across a range of 10–10(8) CFU/mL for P. micra with a limit of detection of 11 CFU/mL.	17	Fluorescence Binding Assay	33.84 ± 0.92 nM	Biosensor	Whole Cell-SELEX	5'-Thiolated and 5'-FAM Labeled	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1923	39905704	2025	Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micra	Apt-ss2	TGACTGACGACGACTC-GGAGAACTACCGCACCAACAATTCACCGTCTGGACGTTCTGCCCTCTCCC-GACTGCTCGAGCTG	80	5'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'	ssDNA	Parvimonas Micra (ATCC 33270)	Whole cell	Isolate aptamers against P. micra and develop colorimetric aptsensor using Au@Fe3O4 nanoparticles.	N/A	17	Fluorescence Binding Assay	N/A	Biosensor	Whole Cell-SELEX	5'-Thiolated and 5'-FAM Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1924	39905704	2025	Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micra	Apt-ss8	TGACTGACGACGACTC-GGGAGGTCAGACTATCTTGTTCCTCCTGGAGGTCGGGAAGAGAGTTGACG-GACTGCTCGAGCTG	80	5'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'	ssDNA	Parvimonas Micra (ATCC 33270)	Whole cell	Isolate aptamers against P. micra and develop colorimetric aptsensor using Au@Fe3O4 nanoparticles.	N/A	17	Fluorescence Binding Assay	N/A	Biosensor	Whole Cell-SELEX	5'-Thiolated and 5'-FAM Labeled	N/A	N/A	N/A	N/A	N/A
ABdb_1925	39905704	2025	Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micra	Apt-ss9	TGACTGACGACGACTC-GGGGGCTGTCAAAAATTACTCTTGGGCCCAGCAATGGTAATTTTCTGACC-GACTGCTCGAGCTG	80	5'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'	ssDNA	Parvimonas Micra (ATCC 33270)	Whole cell	Isolate aptamers against P. micra and develop colorimetric aptsensor using Au@Fe3O4 nanoparticles.	N/A	17	Fluorescence Binding Assay	N/A	Biosensor	Whole Cell-SELEX	5'-Thiolated and 5'-FAM Labeled	N/A	N/A	N/A	N/A	N/A