AptBacterialDB

Modification : 5'-Radiolabelled (32P-labeled)

Total records found: 20

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0009 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF1-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-ACUGUCCUCCCUUCAGAGAGCGCGGGACCCUUAACUUGGGGCCCACGAACAGCUUCAGUUCCGUCUCGGCGU-CAUAUGUGCGUCUACAUGGAUCCUCA1405'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.Addition of aptamer F1-1 in a 1000-fold excess over the colicin E3 CRD completely restored protein synthesis.12Nitrocellulose Filter Binding Assay2 ± 11 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0010 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF2-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-UCCCUGGCCCAAGAUCCUAAUAAAGUUUUUUCGGACCGGAGCGAAACCACUAUCCUCUUAAGCAAUCUGU-CAUAUGUGCGUCUACAUGGAUCCUCA1385'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.Addition of aptamer F2-1 in a 1000-fold excess over the colicin E3 CRD completely restored protein synthesis.12Nitrocellulose Filter Binding Assay14 ± 2 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0011 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF3-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GUACAACACAUCAUUACGGCUGCUAUUGGCUCCAAGCGUCUUUCUCCCUGGUCAAUAGUCCAGCCACCACG-CAUAUGUGCGUCUACAUGGAUCCUCA1395'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.N/A12Nitrocellulose Filter Binding Assay4 ± 15 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0012 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF4-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGAGGGCUCGGACUCUGAUUGCCUCCCCGCACC-CAUAUGUGCGUCUACAUGGAUCCUCA1385'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.N/A12Nitrocellulose Filter Binding Assay7 ± 1 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0013 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF5-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GUCAGCUGCUCGCGGGAUCGAUCCAUUCGGUGGCCAUGCUCCGGAAGAGGCUUCGCAAGACUCAGG-CAUAUGUGCGUCUACAUGGAUCCUCA1345'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.N/A12Nitrocellulose Filter Binding Assay10 ± 1 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0014 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF4-2GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGGGGGCUCGGACUCUGAUUGCCUCCCCGCACC-CAUAUGUGCGUCUACAUGGAUCCUCA1385'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.N/A12Nitrocellulose Filter Binding Assay294 ± 170 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0015 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.4GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UCACUGUUAUCCGAUAGCAGCGCGGGAUGA-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.2.0 μg of RNA aptamer S-PS8.4 effected ca. 71% inhibition of cell invasion by pil+ S. enterica serovar Typhi A21-6 but only ca. 19% inhibition in the case of the pilS::Kmr mutant.8Nitrocellulose Filter Binding Assay8.56 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0016 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.3GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AUUACCUAGAGCGGGAUAAAGUAUAGGUU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0017 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.2GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-CAUCGAGGAGGCGGGAUAUUCGAUGAGUU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0018 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.5GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-GAGUACGAGCGGGAUAGUAAUCGGGUGAU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0019 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.6GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-GAAGGGGUCCGGCUCGGGUGAGGGUCGGGU-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0020 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.9GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AGCUAGCGGGGGGGCUCGACGGUGGUGGGUU-GGGUCAAUGCGUCAUA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0021 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.7GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UAGCGGGAGCUUGGACCUGGUGGUCGCGGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0022 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.1GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UUGGGAUCAGCUCGGCGCUGGAGGAGGGGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0023 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.8GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AACUGUACAUGGGCGCAACAGGGAGUUAGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0356 251855032014Selection of peptidoglycan-specific aptamers for bacterial cells identificationAntibac1TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC555'-TCGCGCGAGTCGTCTG-N24-CCGCATCGTCCTCCC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923) and Escherichia Coli (E. Coli) TOP10PeptidoglycanIdentify aptamers that bind to the peptidoglycan of bacterial cells.High binding affinity for S. aureus, as the radioactivity of Antibac1 was 28 times higher than the negative control.5Saturation Binding Assay0.415 + 0.047 μMDetectionSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0357 251855032014Selection of peptidoglycan-specific aptamers for bacterial cells identificationAntibac2TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC555'-TCGCGCGAGTCGTCTG-N24-CCGCATCGTCCTCCC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923) and Escherichia Coli (E. Coli) TOP10PeptidoglycanIdentify aptamers that bind to the peptidoglycan of bacterial cells.High binding affinity for S. aureus, as the radioactivity of Antibac2 was 22 times higher than the negative control.5Saturation Binding Assay1.261 + 0.280 μMDetectionSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0696 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA20GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 70.86 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0697 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA23GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 61.50 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0698 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA34GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 72.42 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A