AptBacterialDB

Modification : 5'-Radiolabelled ([γ-32P]-ATP-labeled)

Total records found: 19

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0059 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 19TAGGGAATTCGTCACGGATCC-GGCGTCCACTCCCAGCCGCTCACTAGTTTCTGCGTGGGTGA-CTGCAGGTCGACGCATGCGCCG845'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.Survival rate of mice with LPS-induced sepsis increased from 10% to 75% after treatment.12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0060 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 18TAGGGAATTCGTCACGGATCC-GGCGTCCACTCCCAGCCGGTCACTAGTTTCTGCGTGGGTGA-CTGCAGGTCGACGCATGCGCCG845'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0061 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 31TAGGGAATTCGTCACGGATCC-GGAGAATAACGACAAAAGGTAAACTACAGGCCCGGAGC-CTGCAGGTCGACGCATGCGCCG815'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0062 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 14TAGGGAATTCGTCACGGATCC-AAAAGTCCTTGCAAGATAGACGCAGCCAGCCGGGTAGTC-CTGCAGGTCGACGCATGCGCCG825'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0063 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 25TAGGGAATTCGTCACGGATCC-CTTGGCGTTACTTACCTGTACGTCGTAGAG-CTGCAGGTCGACGCATGCGCCG735'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0064 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 43TAGGGAATTCGTCACGGATCC-TGAACGACGTCGCATTAGCGAGTAGGTTTACGAAGTAAGA-CTGCAGGTCGACGCATGCGCCG835'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1038 3056304420182'-Fluoro-Pyrimidine-Modified RNA Aptamers Specific for Lipopolysaccharide Binding Protein (LBP)A011CCAAGCUUGCAUGCCUGCAG-GGACUGUGGAUUGUUCUGGGUGCCGCUUCUCCGAAUAUCGGACUCUCGAACUCCUGGGGA-GGUACCGAGCUCGAAUUCCC1005'-CCAAGCTTGCATGCCTGCAG-N60-GGTACCGAGCTCGAATTCCC-3'ssRNAGram-negative BacteriaMurine Lipopolysaccharide Binding Protein (mLBP)Identify aptamers that bind to and inhibit the LPS-LBP interaction or block of LBP-mediated transfer of LPS monomers to CD14.N/A8Nitrocellulose Filter Binding Assay267 ± 48 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/ABest CandidateN/AN/A
ABdb_1039 3056304420182'-Fluoro-Pyrimidine-Modified RNA Aptamers Specific for Lipopolysaccharide Binding Protein (LBP)A036CCAAGCUUGCAUGCCUGCAG-GAUAUACGGUGCCGCUUCUCUAACCGGUGGUCAUGCGGUUGGACGCUCGAACAGACUA-GGUACCGAGCUCGAAUUCCC985'-CCAAGCTTGCATGCCTGCAG-N60-GGTACCGAGCTCGAATTCCC-3'ssRNAGram-negative BacteriaMurine Lipopolysaccharide Binding Protein (mLBP)Identify aptamers that bind to and inhibit the LPS-LBP interaction or block of LBP-mediated transfer of LPS monomers to CD14.N/A8Nitrocellulose Filter Binding AssayN/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1203 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G1CAGGTCCATCGAGTGGTA-GGAGGTATTTAGTGCCAAGCCATCTCAAACGACGTCTGAG-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)3.1 ± 1.2 nMDetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1204 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G3CAGGTCCATCGAGTGGTA-TGCAGCGGACAGTGTGGGACCATCGCTGCGGATGTATGAA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)5.6 ± 2.4 nMDetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1205 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G7CAGGTCCATCGAGTGGTA-CCCAACCCACGATGCGCAAGAGGAATGCAGCCTACCAGCA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)4.6 ± 1.6 nMDetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/ABest CandidateN/AN/A
ABdb_1206 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G1-T1TAGGAGGAGGTATTTAGTGCCAAGCCATCTCAAACGACGTCTGAGTCGCACTGCTCCTG595'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.Achieved a detection limit of 1 nM.10Electrophoretic Mobility Shift Assay (EMSA)4.5 ± 2.2 nMDetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled) and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1207 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G2CAGGTCCATCGAGTGGTA-CCGAGTTCCCAATATTATGGCTATGCAGGATACTTCACCT-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1208 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G4CAGGTCCATCGAGTGGTA-TGAGTACTAGTTCCCCAGGAGAAAGCAGATCCCCAGGTAC-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1209 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G5CAGGTCCATCGAGTGGTA-GCACAGGACGCAAGATGAATGCAGCATACCAGTCCCTAGA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1210 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G6CAGGTCCATCGAGTGGTA-CAGCTGTCGACGCGTTACCGTGAACGGAACACCGATGACG-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1211 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G8CAGGTCCATCGAGTGGTA-TGCGTGATTGGACCAGGGAAAGATGCACCGCAAGACAAGA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1212 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G9CAGGTCCATCGAGTGGTA-AGGATAATCCGATACGCAAGAAGAAAGCAGATTACCAGGA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1213 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G10CAGGTCCATCGAGTGGTA-CAAAGTCGGCAAGGTGGAAAGCAGCCACACCACGACTAGT-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A