AptBacterialDB

Modification : 5'-Fluorescein Labeled

Total records found: 15

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0398 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-6ATACTATCGCATTCCTTCCGAGGGGGGAGGGGGGGGTGGGGGTCGGT47N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)β-structure of membrane associated proteinImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.Showed the highest binding signals to S. mutans with a 16-fold increase over SMa#3-6-P1.11Flow Cytometry33 nMDetectionIn Silico Maturation (ISM)5'-Fluorescein LabeledN/AN/ABest CandidateN/AN/A
ABdb_0574 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/8ATACCAGCTTATTCAATT-AGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.PA#2/8 competes with IgG or IgG-Fc for binding to Protein A.11Bead-based Binding Assay1.06 ±0.2 μMDetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0575 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/22ATACCAGCTTATTCAATT-GCAGTACTGATGAGTGTAGCCGTATGATTATCGTTTGTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0576 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/34ATACCAGCTTATTCAATT-CCCCAACGAGTCGATATGTAGCCCACACTCTGATTCGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0577 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/11ATACCAGCTTATTCAATT-GGAGACGACAAACTATTACGTACTACGGCATGCACTTGGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0578 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/3ATACCAGCTTATTCAATT-CGACAAGTGGGCATTACGATTCTAGCCCTGATTATGTTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0579 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/6ATACCAGCTTATTCAATT-ACCGATCACTAGCCGACTAATTGGTTTCCGATCGCAGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0580 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#14/82ATACCAGCTTATTCAATT-CCACAACCGAACTCGTAAGACGTATGTAGCCGCCAACTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0581 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/52ATACCAGCTTATTCAATT-ACGCATTGGAGCCCGAAACTGATTCATTGAGCCTACCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0582 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/14ATACCAGCTTATTCAATT-ACGACCGTAGACGACTTACACTGATGTTGCGCATTTCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0583 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/31ATACCAGCTTATTCAATT-CGATGACGACTGTAGCCGCAATACGCCCCTGTTACGTTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0584 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/41ATACCAGCTTATTCAATT-GGACGCCGACTAACTTTACGTGGTTCTCCTACCGCCTAACC-ACAATCGTAATCAGTTAG775'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0585 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/43ATACCAGCTTATTCAATT-ACGAAATGTAGCCGATCCTGATTACTCTCTGTCAGCTTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0962 295947122017Fluorometric graphene oxide-based detection of Salmonella enteritis using a truncated DNA aptamerSE54FTACCAAAATGTTGGATTGGATGTTGTACTGGGTTGCATAGGTAGTCCAGAAGCC54N/AssDNASalmonella Enteritidis (S. Enteritidis) (ATCC 13076)Whole cellTruncated aptamer was used to develop a fluorometric graphene oxide (GO) based assay for the detection of S. enteriditis.LOD of 38 and 25 cfu/mL for the full length SE54 and SE54T, respectively, with a linear response from 10(2) to 10(7) cfu/mL.N/AFluorescence Spectroscopy6.3 nMDetectionN/A5'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0963 295947122017Fluorometric graphene oxide-based detection of Salmonella enteritis using a truncated DNA aptamerSE54TGGATTGGATGTTGTACTGGGTTGCATAGG29N/AssDNASalmonella Enteritidis (S. Enteritidis) (ATCC 13076)Whole cellTruncated aptamer was used to develop a fluorometric graphene oxide (GO) based assay for the detection of S. enteriditis.LOD of 38 and 25 cfu/mL for the full length SE54 and SE54T, respectively with a linear response from 10(2) to 10(7) cfu/mL.N/AFluorescence Spectroscopy3.2 nMDetectionN/A5'-Fluorescein LabeledN/AN/ABest CandidateN/AN/A