Modification : 5'-FAM Labeled and Biotinylated

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_0322	23811206	2013	In vitro selection of a DNA aptamer targeted against Shigella dysenteriae	S 1	ATAGGAGTCACGACGACCAGAA-CGGAACTAGCGTTTAAATGCCAGGACTGAAGTAGGCAGGG-TATGTGCGTCTACCTCTTGACTAAT	87	5'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'	ssDNA	Shigella Dysenteriae	Whole cell	Identify an aptamer targeted against Shigella dysenteriae and develop a sandwich-type fluorescent bioassay for quantification.	Obtained linear range between 10(2)-10(7) cfu/mL of S. dysenteriae, and the limit of detection was 50 cfu/mL	8	Fluorescence Binding Assay	23.47 ± 2.48 nM	Detection	Whole Cell-SELEX	5'-FAM Labeled and Biotinylated	N/A	N/A	Best Candidate	N/A	N/A
ABdb_0713	https://doi.org/10.1039/C5AY00214A	2015	Selection, identification, and application of dual DNA aptamers against Shigella sonnei	Sp1	TGAGCCCAAGCCCTGGTATG-TTCTTCCCTTTTATTAGTCCTGTATTCCTCTACTGTTGCC-GGCAGGTCTACTTTGGGATC	80	5'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'	ssDNA	Shigella Sonnei (ATCC 51334)	Whole cell	Identify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.	With sandwich Sp1 and Sp20, the aptamer assay achieved a concentration range for S. sonnei of 10(2) cfu/mL to 10(7) cfu/mL and a detection limit of 30 cfu/mL.	12	Flow Cytometry	5.98 ± 0.835 nM	Detection	Whole Cell-SELEX	5'-FAM Labeled and Biotinylated	N/A	N/A	N/A	N/A	N/A