AptBacterialDB

Modification : 2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)

Total records found: 3

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0075 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C11GGGAGGAGGAGAGAUGUGAACUU-AUUCGGGCCCAGGAACCAACUAUAUAAAUGUCCCGAAUGCUUCGACG-AGAAACUCUACACUGGACUGGCG935'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 304 ± 13 nM and KI' of 163 nM for C11.9Nitrocellulose Filter Retention Assay186 ± 18 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/ABest CandidateN/AN/A
ABdb_0076 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C12GGGAGGAGGAGAGAUGUGAACUU-ACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCA-AGAAACUCUACACUGGACUGGCG915'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 66 ± 2 nM and KI' of 525 nM for C12.9Nitrocellulose Filter Retention Assay111 ± 12 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/AN/AN/AN/A
ABdb_0077 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C22GGGAGGAGGAGAGAUGUGAACUU-GACAGCGUGCCUAGAAGUCCAAGCUUAAAUAACCACGCUCGACAAGC-AGAAACUCUACACUGGACUGGCG935'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 214 ± 9 nM and KI' of 603 nM for C22.9Nitrocellulose Filter Retention Assay87 ± 20 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/AN/AN/AN/A