AptBacterialDB

Modification : 2'-Fluoro pyrimidines (2'-F-RNA)

Total records found: 40

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0073 195058162009A sensitive method to detect Escherichia coli based on immunomagnetic separation and real-time PCR amplification of aptamersE. coli specific RNA aptamerGGGAGAGCGGAAGCGUGCUGGGUCGCAGUUUGCGCGCGUUCCAAGUUCUCUCAUCACGGAAUACAUAACCCAGAGGUCGAU81N/AssRNAEscherichia Coli (E. Coli) DH5αWhole cellDevelop an aptamer-based, antibody-conjugated magnetic-bead sandwich assay to detect E. coli.Showed a wide dynamic range from 10¹ to 10(7) E. coli per ml and achieved a detection limit of 10 E. coli in a 1 ml sample.N/AN/AN/ABiosensorN/A2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0075 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C11GGGAGGAGGAGAGAUGUGAACUU-AUUCGGGCCCAGGAACCAACUAUAUAAAUGUCCCGAAUGCUUCGACG-AGAAACUCUACACUGGACUGGCG935'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 304 ± 13 nM and KI' of 163 nM for C11.9Nitrocellulose Filter Retention Assay186 ± 18 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/ABest CandidateN/AN/A
ABdb_0076 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C12GGGAGGAGGAGAGAUGUGAACUU-ACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCA-AGAAACUCUACACUGGACUGGCG915'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 66 ± 2 nM and KI' of 525 nM for C12.9Nitrocellulose Filter Retention Assay111 ± 12 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/AN/AN/AN/A
ABdb_0077 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C22GGGAGGAGGAGAGAUGUGAACUU-GACAGCGUGCCUAGAAGUCCAAGCUUAAAUAACCACGCUCGACAAGC-AGAAACUCUACACUGGACUGGCG935'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 214 ± 9 nM and KI' of 603 nM for C22.9Nitrocellulose Filter Retention Assay87 ± 20 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/AN/AN/AN/A
ABdb_0090 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerTwo Arm ( from clone I-1)GGGUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAUAGAUAGUAA645'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Specifically bound to the lipopolysaccharide, which includes the O antigen from the O157:H7 strain, but not to the LPS from the K12 strain.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)~110 nMDetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/ABest CandidateN/AN/A
ABdb_0091 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerClone I-1GGGAUACCAGCUUAUUCAAUU-UGAUUCCAUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAU-AGAUAGUAAGUGCAAUCU995'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Bound to the E. coli O157:H7 strain 2.8-fold better than the control.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/AN/AN/AN/A
ABdb_0092 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerClone III-2GGGAUACCAGCUUAUUCAAUU-CGCACUGUUUUGCCCAGCCGUGUGAAAUUGAUUAGACGUUUUGUAUGCGAUUUCGCCUUG-AGAUAGUAAGUGCAAUCU995'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Bound to the E. coli O157:H7 strain 2.3-fold better than the control.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/AN/AN/AN/A
ABdb_0273 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2GGGAGAGCGGAAGCGUGCUGGG-UAGUGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGA-CAUAACCCAGAGGUCGAUGGUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20.27 ± 4.372 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0274 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2 Truncated 43-merGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGAC395'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)27.64 ± 4.784 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0275 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #2GGGAGAGCGGAAGCGUGCUGGGCC-GGGAGUUUUGAUACGGCUUCAUGCAGUAAUGUUUUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.The level of binding of #2 RNA aptamer to S. aureus was 1.6-fold higher than that of the control aptamer.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/ABest CandidateN/AN/A
ABdb_0276 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #10GGGAGAGCGGAAGCGUGCUGGGCC-GUUAAUACGGUGUCUUUUUCGGUCGUGUAUAAAACGGAAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0277 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #14GGGAGAGCGGAAGCGUGCUGGGCC-UAGGACAGUUCGUCCUCAUUACAUCGCCGCCUAACACAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0278 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #16GGGAGAGCGGAAGCGUGCUGGGCC-UUAGAAGUAGCCUGCUACGCAUGGUCGACUCAAGAAUCG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0279 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #18GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0280 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #21GGGAGAGCGGAAGCGUGCUGGGCC-AGUCUGACACGUAGACAGUUCUAUUACUUACGUCGAGA-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0281 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #23GGGAGAGCGGAAGCGUGCUGGGCC-ACAGUGUUCUAAUGCGACAAUGGAGUCUGUGGCAAAGUGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0282 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #24GGGAGAGCGGAAGCGUGCUGGGCC-UCUCAGGCCGACAUUCUGAGAACGCGAGGCGUAUUGAAG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0283 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #26GGGAGAGCGGAAGCGUGCUGGGCC-UUCAAGUAGGGGCGGUUUACUAUCUGGAUCUUGUAGUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0284 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #07GGGAGAGCGGAAGCGUGCUGGGCC-ACUUGGGGACGACGAGUAGAUAGUAAGGUGGAGACCUGGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0285 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #06GGGAGAGCGGAAGCGUGCUGGGCC-UAUGACAUAAGGUGGGCUGGGAAGCUAGAGCAUGUAAGG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0286 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #05GGGAGAGCGGAAGCGUGCUGGGCC-GUGAAGAAAAAGGGGCGGAUUGGGUAGUAGGGAGGAGAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0287 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #04GGGAGAGCGGAAGCGUGCUGGGCC-AGGAUAGGGGAUGAAGAAAAAAAGAAGGGUGCCGUGGCGC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0288 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone G1GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0446 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmA_Apt1CACAGCGGGACAGUUUAGC-AUUAGCUAUCUUCCACGAUUCGAUCAGGCAGUACGUCGU-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer mA_Apt1 (~40% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/ABest CandidateN/AN/A
ABdb_0447 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmB_Apt23CACAGCGGGACAGUUUAGC-ACAGUUAUCCGACUGCUAUUCGAUCAGGCAGUACGUAGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells. A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer mB_Apt23 (~20% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0448 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmC_Apt41CACAGCGGGACAGUUUAGC-CAGGCUGUUCUGACGCAUAAGGAAUGCGCUGUUGCAGAG-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.mC_Apt41 neutralized 0.007 pmole of Stx2 (≤60% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0449 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmD_Apt46CACAGCGGGACAGUUUAGC-UUGGUCCUGCUUUGGAUAGUCGCGAAAGGGGUGCCACUG-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0450 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspA_Apt1CACAGCGGGACAGUUUAGC-ACAGUUAUCCGACUGCUAUUCGAUCAGGCAGUACGUAGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0451 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspB_Apt14CACAGCGGGACAGUUUAGC-ACCGAGCGGUUUUACGUCUCAAGUAGUAUCCCGUUUUGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Did not inhibit Stx2 binding.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0452 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspC_Apt22CACAGCGGGACAGUUUAGC-AUUAGCUAUCUUCCACGAUUCGAUCAGGCAGUACGUCGU-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer pC_Apt22 (≤60% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0453 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspD_Apt25CACAGCGGGACAGUUUAGC-UUGCCAUCCUGUACUAUGCUCUAUCGGGCGGUUUAGUGAUCCUUCGUCCAACUAUC-GGUAGGUGGGUGCGUCUAAA955'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Did not inhibit Stx2 binding.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0776 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc3 (31) -TruncatedUUCAAUUGCACGAAUUUGCUGUGUUUUUGGG315'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.Ec3(31)-biotin-streptavidin magnetic separation has LOD of 1.3 × 10(6) CFU/ml, Optical analytic technique has LOD of 5 × 10(4) CFU/ml, and Electrochemical Impedance Spectroscopy (EIS) has LOD of 2 × 10(4) CFU/mL of E. coli cells.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)225 nMBiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-Biotinylated or Thiolated or Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0777 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc3AUACCAGCUUAUUCAAUUGCACGAAUUUGCUGUGUUUUUGGGGGGGUCGGGGAGUAUAAGAUAGUAAGUGCAAUCU765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0778 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc2AUACCAGCUUAUUCAAUUCUUCUUUGAUCUGCUCGUAUCAUGGGACGGGAGGGUAUAGAUAGUAAGUGCAAUCU745'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0779 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc5AUACCAGCUUAUUCAAUUCGAAUUUAAGUUUCUUUUCUGGUAUAGUGGUUGGGUGGGUAAGAUAGUAAGUGCAAUCU775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1038 3056304420182'-Fluoro-Pyrimidine-Modified RNA Aptamers Specific for Lipopolysaccharide Binding Protein (LBP)A011CCAAGCUUGCAUGCCUGCAG-GGACUGUGGAUUGUUCUGGGUGCCGCUUCUCCGAAUAUCGGACUCUCGAACUCCUGGGGA-GGUACCGAGCUCGAAUUCCC1005'-CCAAGCTTGCATGCCTGCAG-N60-GGTACCGAGCTCGAATTCCC-3'ssRNAGram-negative BacteriaMurine Lipopolysaccharide Binding Protein (mLBP)Identify aptamers that bind to and inhibit the LPS-LBP interaction or block of LBP-mediated transfer of LPS monomers to CD14.N/A8Nitrocellulose Filter Binding Assay267 ± 48 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/ABest CandidateN/AN/A
ABdb_1039 3056304420182'-Fluoro-Pyrimidine-Modified RNA Aptamers Specific for Lipopolysaccharide Binding Protein (LBP)A036CCAAGCUUGCAUGCCUGCAG-GAUAUACGGUGCCGCUUCUCUAACCGGUGGUCAUGCGGUUGGACGCUCGAACAGACUA-GGUACCGAGCUCGAAUUCCC985'-CCAAGCTTGCATGCCTGCAG-N60-GGTACCGAGCTCGAATTCCC-3'ssRNAGram-negative BacteriaMurine Lipopolysaccharide Binding Protein (mLBP)Identify aptamers that bind to and inhibit the LPS-LBP interaction or block of LBP-mediated transfer of LPS monomers to CD14.N/A8Nitrocellulose Filter Binding AssayN/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1608 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su12GGGAGUCGACCGACCAGAA-CAUACUGAGUAAGAUCGGAAAUUUCGGUGUAAGGCCACGG-UAUGUGCGUCUACAUCUAGACUCAU845'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.Significantly reduced biofilm formation by 61.2%.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/ABest CandidateN/AN/A
ABdb_1609 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su057GGGAGUCGACCGACCAGAA-UGGAUGUAUGGAACUUGCAGAUCUUAACUGCACGAAGCGU-UAUGUGCGUCUACAUCUAGACUCAU845'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.N/A8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_1610 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su15GGGAGUCGACCGACCAGAA-ACACGUUGCUGAAACAUACCGAGUAACAUAAAGCGGGUG-UAUGUGCGUCUACAUCUAGACUCAU835'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.N/A8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A