AptBacterialDB

Method : SELEX and Mod-SELEX

Total records found: 7

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_1896 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS8CGTACGGTCGACGCTAGC-ACACCCCCCAGGATCATAAATTCTCTCGCTGTAGC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1897 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS10CGTACGGTCGACGCTAGC-ACCAGCCATAGTCACATCAAAACTAACTCACATTC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1898 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS12CGTACGGTCGACGCTAGC-CCCATACTCATCACCATTCACATCACTCACCTAGC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1899 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS1GGATCCGAGCTCCACGTG-GCTAGGTGAGTGATGTGAATGGTGATGAGTATGGG-GCTAGCGTCGACCGTACG715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S1, the Ru-modified oligonucleotide, bound S. pneumoniae R6 with slightly lower affinity than S9.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry125 ± 91 nMDetectionSELEX and Mod-SELEXT=dURu(bpy)TP1, 5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1900 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS9ACACCCCCCAGGATCATAAATTCTCTCGCTGTAGC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S9 bound better to the bacterial target than the modified sequence S1.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry118 ± 1 nMDetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1901 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS11ACCAGCCATAGTCACATCAAAACTAACTCACATTC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S11 exhibited a lower propensity at binding to the bacterial target.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry541 ± 1 nMDetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1902 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS13CCCATACTCATCACCATTCACATCACTCACCTAGC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A