AptBacterialDB

Method : SELEX

Total records found: 1461

Download:

Search:
AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0001 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12b10_65GACGTCTTCGAATCCCCATACTGTGGCATTGGCTCAGGACGGTCTGGAGGATGGGGCTTGACGTG655'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)200 ± 20 (against short SEB peptide) and 420 nM (Full length SEB protein)DetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/ABest CandidateN/AN/A
ABdb_0002 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12b10TCAGCTGGACGTCTTCGAAT-CCCCATACTGTGGCATTGGCTCAGGACGGTCTGGAGGATGGGGCTTGACGTCATCATCTG-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0003 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12a10TCAGCTGGACGTCTTCGAAT-TACCAAGGTGTACGAGCGGCATTGGCTTAGGATGGTCTAGGAGAACGCCTTGTTCACGGG-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0004 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12d10TCAGCTGGACGTCTTCGAAT-GGGCATTGGCTAGGACGGTCTAGCAGGAGAAGATGTTAAGTGTGCCTTAGTCAGAGAGTG-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0005 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BB12e7TCAGCTGGACGTCTTCGAAT-GGGTATAGTGTCTGGCATTGGCGAGGATGGTCTCAAACGCTAAACCCAATCTGTATAACA-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0006 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BA8c3TCAGCTGGACGTCTTCGAAT-CGTGGGCATCGGGCTGCATGAATGGCATTGGCGAGGAGGGTGTGTTTGTAGGCAGCACCT-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0007 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BAE11a5TCAGCTGGACGTCTTCGAAT-ACCCGTACAGTCCTATGTTGGCATCCTCACTTGTGGCATTGGCAAGGATGGTCTTCTAG-TGTCAGGAGCTCGAATTCCC995'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0008 127994282003A DNA Spiegelmer to staphylococcal enterotoxin BA8g1TCAGCTGGACGTCTTCGAAT-GCTCGACAGGGCACCACCGCCTCGGCATTGGCTAGGTTGGTCTCTTTGGCGGGGTTGCGC-TGTCAGGAGCTCGAATTCCC1005'-TCAGCTGGACGTCTTCGAAT-N60-TGTCAGGAGCTCGAATTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (Full-length protein and short SEB peptides)Identify aptamers against the L-configured peptide and the full-length SEB protein.N/A12Surface Plasmon Resonance (SPR)N/ADetectionMirror-Image SELEXL-DNA SpiegelmerN/AN/AN/AN/AN/A
ABdb_0009 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF1-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-ACUGUCCUCCCUUCAGAGAGCGCGGGACCCUUAACUUGGGGCCCACGAACAGCUUCAGUUCCGUCUCGGCGU-CAUAUGUGCGUCUACAUGGAUCCUCA1405'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.Addition of aptamer F1-1 in a 1000-fold excess over the colicin E3 CRD completely restored protein synthesis.12Nitrocellulose Filter Binding Assay2 ± 11 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0010 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF2-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-UCCCUGGCCCAAGAUCCUAAUAAAGUUUUUUCGGACCGGAGCGAAACCACUAUCCUCUUAAGCAAUCUGU-CAUAUGUGCGUCUACAUGGAUCCUCA1385'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.Addition of aptamer F2-1 in a 1000-fold excess over the colicin E3 CRD completely restored protein synthesis.12Nitrocellulose Filter Binding Assay14 ± 2 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0011 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF3-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GUACAACACAUCAUUACGGCUGCUAUUGGCUCCAAGCGUCUUUCUCCCUGGUCAAUAGUCCAGCCACCACG-CAUAUGUGCGUCUACAUGGAUCCUCA1395'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.N/A12Nitrocellulose Filter Binding Assay4 ± 15 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0012 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF4-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGAGGGCUCGGACUCUGAUUGCCUCCCCGCACC-CAUAUGUGCGUCUACAUGGAUCCUCA1385'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.N/A12Nitrocellulose Filter Binding Assay7 ± 1 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0013 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF5-1GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GUCAGCUGCUCGCGGGAUCGAUCCAUUCGGUGGCCAUGCUCCGGAAGAGGCUUCGCAAGACUCAGG-CAUAUGUGCGUCUACAUGGAUCCUCA1345'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.N/A12Nitrocellulose Filter Binding Assay10 ± 1 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0014 150230712004In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNAF4-2GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGGGGGCUCGGACUCUGAUUGCCUCCCCGCACC-CAUAUGUGCGUCUACAUGGAUCCUCA1385'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'ssRNAEscherichia Coli (E. Coli)C-terminal ribonuclease domain of Colicin E3Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.N/A12Nitrocellulose Filter Binding Assay294 ± 170 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0015 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.4GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UCACUGUUAUCCGAUAGCAGCGCGGGAUGA-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.2.0 μg of RNA aptamer S-PS8.4 effected ca. 71% inhibition of cell invasion by pil+ S. enterica serovar Typhi A21-6 but only ca. 19% inhibition in the case of the pilS::Kmr mutant.8Nitrocellulose Filter Binding Assay8.56 nMTherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0016 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.3GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AUUACCUAGAGCGGGAUAAAGUAUAGGUU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0017 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.2GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-CAUCGAGGAGGCGGGAUAUUCGAUGAGUU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0018 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.5GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-GAGUACGAGCGGGAUAGUAAUCGGGUGAU-GGGUCAAUGCGUCAUA875'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0019 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.6GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-GAAGGGGUCCGGCUCGGGUGAGGGUCGGGU-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0020 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.9GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AGCUAGCGGGGGGGCUCGACGGUGGUGGGUU-GGGUCAAUGCGUCAUA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0021 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.7GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UAGCGGGAGCUUGGACCUGGUGGUCGCGGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0022 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.1GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-UUGGGAUCAGCUCGGCGCUGGAGGAGGGGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0023 161890802005Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhiS-PS8.8GCGGAAUUCUAAUACGACUCACUAUAGGGAACAGUCCGAGCC-AACUGUACAUGGGCGCAACAGGGAGUUAGC-GGGUCAAUGCGUCAUA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssRNASalmonella TyphiType IVB Pili structural proteinsIdentify an aptamer that binds to type IVB pili and inhibits the entry of the piliated strain (but not that of the nonpiliated strain) into human THP-1 cells.N/A8Nitrocellulose Filter Binding AssayN/ATherapeuticsSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0024 171888712007In vitro selection of RNA aptamer against Escherichia coli release factor 1Class II-1GGACCGAGAAGUUACCCUGUAAUCUUAGGAUGAAUCGCAUGCUCUAGCGACCUUUUCGGCUUCGGCGUACGCACAUCGCAGCAAC85N/AssRNAEscherichia Coli (E. Coli)Release factor 1 (RF-1)Identify aptamers that bind to and inhibit the action of RF-1 to enhance the efficiency of nonsense suppression.Aptamer class II-1 (12 μM) increased the suppression efficiency (44% → 89%).11Surface Plasmon Resonance (SPR)30 ± 6 nMTherapeuticsSELEX3'-Biotinylated (Biotin-d(A)11) or 3'-TexasRed LabeledN/AN/AN/AN/AN/A
ABdb_0025 172651802007Fluorescence assay based on aptamer-quantum dot binding to Bacillus thuringiensis sporesAptamerCATCCGTCACACCTGCTCTGGCCACTAACATGGGGACCAGGTGGTGTTGGCTCCCGTATC60N/AssDNABacillus Thuringiensis (BT)BT sporesAptamer coupled to fluorescent zinc sulfide-capped, cadmium selenide quantum dots (QD) for detecting BT spores.Limit of detection (LOD) between 10³ and 10(4) CFU.N/AN/AN/ABiosensorSELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0026 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL1FATCCGTCACCCCTGCTCTCGTCGCTATGAAGTAACAAAGATAGGAGCAATCGGGTGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0027 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL3FATCCGTCACACCTGCTCTAACGAAGACTGAAACCAAAGCAGTGACAGTGCTGAATGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0028 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL4FATCCGTCACACCTGCTCTCGGTGACAATAGCTCGATCAGCCCAAAGTCGTCAGATGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0029 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL6FATCCGTCACACCTGCTCTAACGAAATAGACCACAAATCGATACTTTATGTTATTGGTGTTGGCTCCCGTAT715'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0030 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL7FATCCGTCACACCTGCTCTGTCGAATGCTCTGCCTGGAAGAGTTGTTAGCAGGGATGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0031 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL8FATCCGTCACACCTGCTCTTAAGCCGAGGGGTAAATCTAGGACAGGGGTCCATGATGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0032 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL9FATCCGTCACACCTGCTCTACTGGCCGGCTCAGCATGACTAAGAAGGAAGTTATGTGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0033 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL10FATCCGTCACACCTGCTCTGGTACGAATCACAGGGGATGCTGGAAGCTTGGCTCTTGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0034 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL1RATACGGGAGCCAACACCACCCGATTGCTCCTATCTTTGTTACTTCATAGCGACGAGAGCAGGGGTGACGGAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0035 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL3RATACGGGAGCCAACACCATTCAGCACTGTCACTGCTTTGGTTTCAGTCTTCGTTAGAGCAGGTGTGACGGAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0036 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL4RATACGGGAGCCAACACCATCTGACGACTTTGGGCTGATCGAGCTATTGTCACCGAGAGCAGGTGTGACGGAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0037 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL6RATACGGGAGCCAACACCAATAACATAAAGTATCGATTTGTGGTCTATTTCGTTAGAGCAGGTGTGACGGAT715'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0038 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL7RATACGGGAGCCAACACCATCCCTGCTAACAACTCTTCCAGGCAGAGCATTCGACAGAGCAGGTGTGACGGAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0039 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL8RATACGGGAGCCAACACCATCATGGACCCCTGTCCTAGATTTACCCCTCGGCTTAAGAGCAGGTGTGACGGAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0040 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL9RATACGGGAGCCAACACCACATAACTTCCTTCTTAGTCATGCTGAGCCGGCCAGTAGAGCAGGTGTGACGGAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0041 187591122008In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexesL10RATACGGGAGCCAACACCAAGAGCCAAGCTTCCAGCATCCCCTGTGATTCGTACCAGAGCAGGTGTGACGGAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) O111:B4 and K12 strainsLipopolysaccharide (LPS) and Whole cellIdentify aptamers against LPS and develop Apt-C1qrs complexes to inhibit bacterial growth by triggering the classical complement cascade and rapid passive immunity.Significantly reduced the colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10× dilutions of the bacteria.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0042 188033932008Selection of aptamers against live bacterial cellshemag1PAGCAGCACAGAGGTCAGATG-TAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGTG-CCTATGCGTGCTACCGTGAA785'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).The number of aptamer molecules binding to each cell (on average, ∼164 ± 47 aptamer molecules per cell).8Flow Cytometry13 ± 3 nMDetectionSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0043 188033932008Selection of aptamers against live bacterial cellshemag1TAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGATG395'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0044 188033932008Selection of aptamers against live bacterial cellsmag1TGAGCCCCACTAAAGTTGCAATCATGTCGTCAGCTTTGGG405'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0045 188033932008Selection of aptamers against live bacterial cellshemag3CGTCGCGGCATATTTCCAGTGGAACGGTTACGATATGTTG405'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0046 188033932008Selection of aptamers against live bacterial cellsmag1PAGCAGCACAGAGGTCAGATG-TGAGCCCCAGTAAAGTTGCAATCATGTCGTCAGCTTTGGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0047 188033932008Selection of aptamers against live bacterial cellshemag3PAGCAGCACAGAGGTCAGATG-TCGTCGCGGCATATTTCCAGTGGAACGGTTACGATATGTTG-CCTATGCGTGCTACCGTGAA815'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0048 182148752008Detection and titer estimation of Escherichia coli using aptamer-functionalized single-walled carbon-nanotube field-effect transistorsAptamerGGGAGAGCGGAAGCGUGCUGGGUCGCAGUUUGCGCGCGUUCCAAGUUCUCUCAUCACGGAAUACAUAACCCAGAGGUCGAU81N/AssRNAEscherichia Coli (E. Coli) DH5αWhole cellDevelop aptamer-functionalized SWNT-FET sensors for the detection of E. coli.The SWNT-FETs showed a conductance decrease of more than 50% after binding with E. coli in less than 20 min.N/AN/AN/ADetectionSELEX3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0049 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA20GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellIdentify aptamers that bind to S. aureus strains, including 8325-4, 196, and 04018, and develop a probe to distinguish them from S. epidermidis, Streptococcus, and E. coli.EC50 = 70.86 ± 39.22nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0050 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA23GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 = 61.50 ± 22.43nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0051 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA31GCAATGGTACGGTACTTCC-TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50=82.86 ± 33.20nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0052 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA34GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 =72.42 ± 35.23nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0053 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA43GCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 = 210.70 ± 135.91nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0054 186712602009Preliminary development of DNA aptamer-Fc conjugate opsoninsα-PDGA 2FCATCCGTCACACCTGCTCTG-GTTCGCCCCGGTCAAGGAGA-GTGGTGTTGGCTCCCGTATC605'-CATCCGTCACACCTGCTCTG-N20-GTGGTGTTGGCTCCCGTATC-3'ssDNABacillus Anthracis (BA)Poly-alpha-D-glutamic acid (α-PDGA-coated magnetic beads (MBs)) part of the B.anthracis capsuleIdentify aptamers against α-PDGA-MBs and couple them to Fc fragments of murine IgG DNA to act as potential opsonins.Increase in phagocytic index (P.I.) up to threefold in the first 30 min of exposure to α-PDGA-MBs.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-Amidation (NH₂) and 5'-BiotinylatedCell viability ranged from 90% to 95% in all experiments.N/ABest CandidateN/AN/A
ABdb_0055 186712602009Preliminary development of DNA aptamer-Fc conjugate opsoninsα-PDGA 5RGATACGGGAGCCAACACCAC-TAACTTGTTGCTGATCTTAT-CAGAGCAGGTGTGACGGATG605'-CATCCGTCACACCTGCTCTG-N20-GTGGTGTTGGCTCCCGTATC-3'ssDNABacillus Anthracis (BA)Poly-alpha-D-glutamic acid (α-PDGA-coated magnetic beads (MBs)) part of the B.anthracis capsuleIdentify aptamers against α-PDGA-MBs and couple them to Fc fragments of murine IgG DNA to act as potential opsonins.Increase in phagocytic index (P.I.) up to threefold in the first 30 min of exposure to α-PDGA-MBs.5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-Amidation (NH₂) and 5'-BiotinylatedCell viability ranged from 90% to 95% in all experiments.N/ABest CandidateN/AN/A
ABdb_0056 186712602009Preliminary development of DNA aptamer-Fc conjugate opsoninsα-PDGA 2RGATACGGGAGCCAACACCAC-TCTCCTTGACCGGGGCGAAC-CAGAGCAGGTGTGACGGATG605'-CATCCGTCACACCTGCTCTG-N20-GTGGTGTTGGCTCCCGTATC-3'ssDNABacillus Anthracis (BA)Poly-alpha-D-glutamic acid (α-PDGA-coated magnetic beads (MBs)) part of the B.anthracis capsuleIdentify aptamers against α-PDGA-MBs and couple them to Fc fragments of murine IgG DNA to act as potential opsonins.N/A5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-Amidation (NH₂) and 5'-BiotinylatedCell viability ranged from 90% to 95% in all experiments.N/AN/AN/AN/A
ABdb_0057 186712602009Preliminary development of DNA aptamer-Fc conjugate opsoninsα-PDGA 5FCATCCGTCACACCTGCTCTG-GATAAGATCAGCAACAAGTTA-GTGGTGTTGGCTCCCGTATC615'-CATCCGTCACACCTGCTCTG-N20-GTGGTGTTGGCTCCCGTATC-3'ssDNABacillus Anthracis (BA)Poly-alpha-D-glutamic acid (α-PDGA-coated magnetic beads (MBs)) part of the B.anthracis capsuleIdentify aptamers against α-PDGA-MBs and couple them to Fc fragments of murine IgG DNA to act as potential opsonins.N/A5Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ATherapeuticsMagnetic Bead (MB)-based SELEX5'-Amidation (NH₂) and 5'-BiotinylatedCell viability ranged from 90% to 95% in all experiments.N/AN/AN/AN/A
ABdb_0058 197514192009Antibiotic resistance in bacteria: novel metalloenzyme inhibitorsMetallo-β-lactamase-targeting aptamersCGCGAGCTCCGCGCG-AACCAAACTTGGATCGGTGCACATGTCGAA-CGCGCGCATATGGCGC615'-CGCGAGCTCCGCGCG-N30-CGCGCGCATATGGCGC-3'ssDNABacillus Cereus 5/B/6 and Escherichia Coli (E. Coli) TAP56Metallo-β-lactamase active sitesIdentify aptamers that bind and inhibit the hydrolytic enzyme activity by interfering with the active-site metal ions of the β-lactamase enzyme.LC50 values in the presence of 5 μM cephalexin: 75 μM and 32 μM for B. cereus 5/B/6 and E. coli TAP56, respectively.21Metallo-β-lactamase activity assaysKi = 0.92 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0059 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 19TAGGGAATTCGTCACGGATCC-GGCGTCCACTCCCAGCCGCTCACTAGTTTCTGCGTGGGTGA-CTGCAGGTCGACGCATGCGCCG845'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.Survival rate of mice with LPS-induced sepsis increased from 10% to 75% after treatment.12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0060 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 18TAGGGAATTCGTCACGGATCC-GGCGTCCACTCCCAGCCGGTCACTAGTTTCTGCGTGGGTGA-CTGCAGGTCGACGCATGCGCCG845'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0061 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 31TAGGGAATTCGTCACGGATCC-GGAGAATAACGACAAAAGGTAAACTACAGGCCCGGAGC-CTGCAGGTCGACGCATGCGCCG815'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0062 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 14TAGGGAATTCGTCACGGATCC-AAAAGTCCTTGCAAGATAGACGCAGCCAGCCGGGTAGTC-CTGCAGGTCGACGCATGCGCCG825'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0063 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 25TAGGGAATTCGTCACGGATCC-CTTGGCGTTACTTACCTGTACGTCGTAGAG-CTGCAGGTCGACGCATGCGCCG735'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0064 192656722009A novel lipopolysaccharide-antagonizing aptamer protects mice against endotoxemiaAptamer 43TAGGGAATTCGTCACGGATCC-TGAACGACGTCGCATTAGCGAGTAGGTTTACGAAGTAAGA-CTGCAGGTCGACGCATGCGCCG835'-TAGGGAATTCGTCACGGATCC-N40-CTGCAGGTCGACGCATGCGCCG-3'ssDNAGram-negative BacteriaEndotoxin (Lipopolysaccharide (LPS))Identify aptamers that bind to and inhibit LPS-induced sepsis by decreasing NF-κB activation of monocytes.N/A12Nitrocellulose Filter Binding AssayN/ADiagnostic/TherapeuticsSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_0065 192842972009The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosisM64CA (aptamers 20, 34, 41, 46, and 50 )GGGAGCTCAGAATAAACGCTCAA-TTCGGGAATGATTATCAAATTTATGCCCTCTGAT-TTCGACATGAGGCCCGGATC775'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinIdentify an aptamer that binds to MPT64, and an aptamer-MPT64 was designed to detect M. tuberculosis by measuring MPT64 protein levels in culture filtrates.The sandwich assay scheme based on the aptamer-protein complex had a high sensitivity (86.3%) and specificity (88.5%).12N/AN/ADetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0066 192842972009The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosisAptamer 11 (named M64RA)GGGAGCTCAGAATAAACGCTCAA-TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinIdentify an aptamer that binds to MPT64, and an aptamer-MPT64 was designed to detect M. tuberculosis by measuring MPT64 protein levels in culture filtrates.The sandwich assay scheme based on the aptamer-protein complex had a high sensitivity (86.3%) and specificity (88.5%).12N/AN/ADetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0067 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 33TTTGGTCCTTGTCTTATGTCCAGAATGC-TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.In a pull-down assay format, detection limits were 10¹–10² CFU S. Typhimurium/9 mL rinsate, while in a recirculation format, detection limits were 10²–10³ CFU/25 mL rinsate.7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0068 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 23TTTGGTCCTTGTCTTATGTCCAGAATGC-CCGCCTTTACTAAATTGACGAACATAGGAATCAATGAAGC-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.N/A7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0069 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 24TTTGGTCCTTGTCTTATGTCCAGAATGC-GGGAGTCAGAACGCCTGGGCAAGCATAGTACTCGCCGGAA-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.N/A7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0070 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 25TTTGGTCCTTGTCTTATGTCCAGAATGC-TAAAGAGGAATCGACAGGCATCAGAGTCCGTAATGCGGGG-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.N/A7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0071 190498622009Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovarsAptamer 45TTTGGTCCTTGTCTTATGTCCAGAATGC-GAGGAAAGTCTATAGCAGAGGAGATGTGTGAACCGAGTAA-ATTTCTCCTACTGGGATAGGTGGATTAT965'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNASalmonella Typhimurium (S. Typhimurium) DT104Outer membrane protein (OMP)Identify aptamers that capture and detect Salmonella enterica serovar. Typhimurium.N/A7Electrophoretic Mobility Shift Assay (EMSA)N/ABiosensorSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0074 192348742009Aptamer-based electrochemical biosensor for Botulinum neurotoxinBoNT/A aptamerATACCAGCTTATTCAATTGACATGACTGGGATTTTTGGCGAAATCGAAGGAAGCGGAGAGATAGTAAGTGCAATCT76N/AssDNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A) toxoidDeveloped an aptamer-based electrochemical sensor for the detection of Botulinum neurotoxin.Achieved a limit of detection (LOD) of 40 pg/ml within 24 h.N/AN/A3.0 ± 0.8 × 10(9) M−1BiosensorSingle micro-bead SELEX5'-Biotinylated and 3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0075 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C11GGGAGGAGGAGAGAUGUGAACUU-AUUCGGGCCCAGGAACCAACUAUAUAAAUGUCCCGAAUGCUUCGACG-AGAAACUCUACACUGGACUGGCG935'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 304 ± 13 nM and KI' of 163 nM for C11.9Nitrocellulose Filter Retention Assay186 ± 18 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/ABest CandidateN/AN/A
ABdb_0076 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C12GGGAGGAGGAGAGAUGUGAACUU-ACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCA-AGAAACUCUACACUGGACUGGCG915'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 66 ± 2 nM and KI' of 525 nM for C12.9Nitrocellulose Filter Retention Assay111 ± 12 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/AN/AN/AN/A
ABdb_0077 204523282010In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxinS132B-C22GGGAGGAGGAGAGAUGUGAACUU-GACAGCGUGCCUAGAAGUCCAAGCUUAAAUAACCACGCUCGACAAGC-AGAAACUCUACACUGGACUGGCG935'-GGGAGGAGGAGAGATGTGAACTT-N47-AGAAACTCTACACTGGACTGGCG-3'ssRNAClostridium BotulinumBotulinum neurotoxins (BoNT)-light chain of type A (BoNT/A)Identify aptamers against the light chain of type A BoNT (BoNT/A) and inhibit the endopeptidase activity.IC50 values of 214 ± 9 nM and KI' of 603 nM for C22.9Nitrocellulose Filter Retention Assay87 ± 20 nMTherapeuticsSELEX2'-Fluoro pyrimidines (2'-F-RNA) and Radiolabelled ([α-33P]-GTP labeled)N/AN/AN/AN/AN/A
ABdb_0080 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 4RATCCGTCACACCTGCTCT-ACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA-TGGTGTTGGCTCCCGTAT735'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.Detect as few as 30 live, unlabeled E. coli per ml using FRET assay.5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0081 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 3RATCCGTCACACCTGCTCT-GTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCG-TGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.N/A5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/AN/AN/AN/A
ABdb_0082 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 8FATACGGGAGCCAACACCA-CGACTAACACGACCGTTGGGGGGGGCTCGCGCGGGC-AGAGCAGGTGTGACGGAT725'-ATACGGGAGCCAACACCA-N36-AGAGCAGGTGTGACGGAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.N/A5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/AN/AN/AN/A
ABdb_0083 https://doi.org/10.4028/www.scientific.net/KEM.439-440.14562010In Vitro Selection of Oligonucleotide Acid Aptamers against Pathogenic Vibrio Alginolyticus by SELEXSequence AGCCCAATCGCCGTCTTCTACA-TGTGCGTGTGTCAGCGAATATCACCTAGAGGTCGG-AGTGCCAACGCCCTCCTGAT765'-GCCCAATCGCCGTCTTCTACA-N35-AGTGCCAACGCCCTCCTGAT-3'ssDNAVibrio AlginolyticusWhole cellIdentify a high-affinity aptamer against Vibrio alginolyticus.Bind to different sites of the V. alginolyticus surface.15N/AN/ADetectionSELEX5'-DIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0084 https://doi.org/10.4028/www.scientific.net/KEM.439-440.14562010In Vitro Selection of Oligonucleotide Acid Aptamers against Pathogenic Vibrio Alginolyticus by SELEXSequence BCCCAATCGCCGTCTTCTACA-TGTATGCTGCCGAGGTTGGCCTCTTAGTCAGCTGAGAGTGCCAACGCCCTCCTGATATGCCCAATCGCCGTCTTCTACATTGTGGTCGTGGGAATGCATTCATCCGTTGCCGG-AGTGCCAACGCCCTCC1495'-GCCCAATCGCCGTCTTCTACA-N35-AGTGCCAACGCCCTCCTGAT-3'ssDNAVibrio AlginolyticusWhole cellIdentify a high-affinity aptamer against Vibrio alginolyticus.Bind to different sites of the V. alginolyticus surface.15N/AN/ADetectionSELEX5'-DIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0085 213817552011Aptamer-mediated inhibition of Mycobacterium tuberculosis polyphosphate kinase 2PPK2 G9CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTC-AACACATAGGTTTGGTTAGGTTGGTTGGTTGAATTA-AAGCTTTGCAGAGAGGATCCTT995'-CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTC-N35-AAGCTTTGCAGAGAGGATCCTT-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)PPK2 EnzymeIdentify aptamers that bind to and inhibit the catalytic activities of enzyme PPK2.Inhibited PPK2 protein with an IC50 of 39.3 ± 10 nM and inhibited the NDK activities of both the LHK and VC PPK2 proteins with IC50 values of >1 μM and 105 ± 5 nM, respectively.20Isothermal Titration Calorimetry (ITC)870 ± 220 nMTherapeuticsSELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0086 216274662011Aptamer selection for the detection of Escherichia coli K88Aptamer 19CGTACGGTCGACGCTAGC-GGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTC-CACGTGGAGCTCGGATCC965'-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3'ssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)K88 fimbriae proteinIdentify aptamers that bind to and detect the K88 fimbriae protein of E.coli K88 surface protein.N/A11Fluorescence Spectroscopy44 ± 7 nMDetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0087 216274662011Aptamer selection for the detection of Escherichia coli K88Aptamer 31CGTACGGTCGACGCTAGC-ACACTCTTTTGCTCGTGTTTTTGCCTGTTACATAAAATGAATCAGTGGATGTTTCCTTCT-CACGTGGAGCTCGGATCC965'-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3'ssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)K88 fimbriae proteinIdentify aptamers that bind to and detect the K88 fimbriae protein of E.coli K88 surface protein.N/A11Fluorescence Spectroscopy36 ± 8 nMDetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0088 216274662011Aptamer selection for the detection of Escherichia coli K88Aptamer 7CGTACGGTCGACGCTAGC-GGCGGCCGTGAAATTTGCCAAATGCCGTCTTGGCTTTCGCCCAATGTATCCTGGGTGTTC-CACGTGGAGCTCGGATCC965'-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3'ssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)K88 fimbriae proteinIdentify aptamers that bind to and detect the K88 fimbriae protein of E.coli K88 surface protein.N/A11Fluorescence Spectroscopy43 ± 6 nMDetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0089 216274662011Aptamer selection for the detection of Escherichia coli K88Aptamer 37CGTACGGTCGACGCTAGC-GGAGACCGTACCATCTGTTCGTGGAAGCGCTTTGCTCGTCCATTAGCCTTGTGCTCGTGC-CACGTGGAGCTCGGATCC965'-CGTACGGTCGACGCTAGC-N60-CACGTGGAGCTCGGATCC-3'ssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)K88 fimbriae proteinIdentify aptamers that bind to and detect the K88 fimbriae protein of E.coli K88 surface protein.Fluorescence intensity of other bacteria (ETEC K99, S. aureus, E. coli TOP10) is lower than that of ETEC K88.11Fluorescence Spectroscopy25 ± 4 nMDetectionSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0090 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerTwo Arm ( from clone I-1)GGGUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAUAGAUAGUAA645'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Specifically bound to the lipopolysaccharide, which includes the O antigen from the O157:H7 strain, but not to the LPS from the K12 strain.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)~110 nMDetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/ABest CandidateN/AN/A
ABdb_0091 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerClone I-1GGGAUACCAGCUUAUUCAAUU-UGAUUCCAUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAU-AGAUAGUAAGUGCAAUCU995'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Bound to the E. coli O157:H7 strain 2.8-fold better than the control.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/AN/AN/AN/A
ABdb_0092 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerClone III-2GGGAUACCAGCUUAUUCAAUU-CGCACUGUUUUGCCCAGCCGUGUGAAAUUGAUUAGACGUUUUGUAUGCGAUUUCGCCUUG-AGAUAGUAAGUGCAAUCU995'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Bound to the E. coli O157:H7 strain 2.3-fold better than the control.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/AN/AN/AN/A
ABdb_0093 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A24PAGCAGCACAGAGGTCAGATG-GGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGG-CCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.20A24P forms a branched structure with high affinity for the target cell mixture (gated fluorescence intensity above library of 64%).20Flow Cytometry9.1 ± 0.8 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0094 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes15A3PTTCACGGTAGCACGCATAGG-GACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGG-CATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.Show a gated fluorescence above the background of 48%.20Flow Cytometry9.6 ± 0.3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0095 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A1CAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCC40N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.72% gated fluorescence intensity above the library.20Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0096 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A1PTTCACGGTAGCACGCATAGG-CAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCC-CATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.72% gated fluorescence intensity above the library.20Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0097 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A8CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC40N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.20A8 binding to the target GAS cells yielded 72% gated fluorescence above background.20Flow Cytometry4 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0098 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A8PAGCAGCACAGAGGTCAGATG-CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC-CCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.20A8P binding to the target GAS cells yielded 68% gated fluorescence above background.20Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0099 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A9CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG39N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.65% gated fluorescence intensity above the library.20Flow Cytometry9 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0100 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A9PAGCAGCACAGAGGTCAGATG-CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG-CCTATGCGTGCTACCGTGAA79N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.66% gated fluorescence intensity above the library.20Flow Cytometry9.1 ± 0.6 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0101 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A12PTTCACGGTAGCACGCATAGG-GCCCGACACTCGTCCACCCGATACCTCTCATGTGTCCC-CATCTGACCTCTGTGCTGCT78N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.N/A20Flow Cytometry25 ± 3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0102 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A14PAGCAGCACAGAGGTCAGATG-GGCATGGGGAAGAGAAAGCGGGATAACTTCGTTACCGGGC-CCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.N/A20Flow Cytometry17 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0103 217439202011Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties1–PAGCGCGGATCCCGCGC-GATGTGGGTGTAGTTGGAGGGTAAACGTT-CGCGCGAAGCTTGCG595'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNABacillus Anthracis (BA)Protective antigen (PA) of anthrax toxinDetection of PA based on the reduction in the fluorescence emission according to the formation of the aptamer-PA ternary complex.The limit of PA detection using this system was determined to be approximately 1 nM.8Fluorescence Microplate Reader (Fluorescence Spectroscopy)8.53 nMBiosensorSELEXN/AN/AN/AN/AN/AKorea Pat., KR101152354B1 (2012-06-13)
ABdb_0104 217439202011Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties2–PAGCGCGGATCCCGCGC-CAGACCGTAAGGGATGCCGCCTAAACACC-CGCGCGAAGCTTGCG595'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNABacillus Anthracis (BA)Protective antigen (PA) of anthrax toxinDetection of PA based on the reduction in the fluorescence emission according to the formation of the aptamer-PA ternary complex.The limit of PA detection using this system was determined to be approximately 1 nM.8Fluorescence Microplate Reader (Fluorescence Spectroscopy)1.51 nMBiosensorSELEXN/AN/AN/AN/AN/AKorea Pat., KR101152354B1 (2012-06-13)
ABdb_0105 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF23ATACCAGCTTATTCAATT-CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.F23 showed great specificity to P. aeruginosa and no cross-reactivity with other bacterial species.15Flow Cytometry17.27 ± 5.00 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0106 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF12ATACCAGCTTATTCAATT-CCCCCGTTGCTTTCGCTTTTCCTTTTGCTTTTGTTCGCTTCGTCCCTGCTTCCTTTCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0107 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF8ATACCAGCTTATTCAATT-CCGTGGCGTTCTGTTTGTTCGTTGTTTTTTGGTTTTTCCTTCTTTTTTTGTTTTTGGTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0108 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF1ATACCAGCTTATTCAATT-GGCGCGCGTTGTGTGGTTTGGCTTTTTTCGTTTGTTTTTCTTGGGTGCTTGTCTTCGTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0109 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF20ATACCAGCTTATTCAATT-CCCTCGCCGTACGTTCTCCGTTTTGTCAGGTGTTGTTTTTTCCGTCCTCTTCTCGTGTGC-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0110 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF4ATACCAGCTTATTCAATT-CCACCGATTCCCTTCGATCGTATTCGTGTTGCTCTCGTGTTGTAATGCGTCCTGTGCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0111 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF6ATACCAGCTTATTCAATT-GGCACCGGCTTGTCTGTTTCTCTTGGTTCGCCGCTGGACTTTGTTAGCTCCCTCCGCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0112 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF7ATACCAGCTTATTCAATT-CCCCACAACCTGACCTTTCTATTCCGTTCCCCTTTTTTCAGCTTCTTTCCGGCCTCTGTT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0113 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF18ATACCAGCTTATTCAATT-CCACCACCGGGTTGTTTTTCCTGCAGGCCTTTATCGTTTTCCGCGCTCGATTCGGTCATG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0114 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF5ATACCAGCTTATTCAATT-CCACCGTGCTTTATATTACCCGTCCACCCTTCGGTCTTTCTCCCCGTGTCGCTCCGCTTT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0115 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF21ATACCAGCTTATTCAATT-CCACCGGGCCTCGTATTCGTACTGTTTGATTCTTTGGCTTTGTAGCGGACGTACTGGGTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0116 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF2ATACCAGCTTATTCAATT-CCCCCGCGCCCGTCTCTCTCAATCTCCGCATTCTTTAGTTCTCATCCTCCCTGTGTGTTT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0117 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF24ATACCAGCTTATTCAATT-GACACCCGGAGTAATTCCGTTTTAAGCGTCTTTCATGTGTTCCCTTTCGTTCCTCCCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0118 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF15ATACCAGCTTATTCAATT-CCCAAGGTCGAGTTCCTAGTCTTACCGTATTTCACTTTCCTGGTTCTTACCTCCCCCTCA-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0119 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF22ATACCAGCTTATTCAATT-CGGCGCGAGTCGACTGCACAGGGTCCTATTGCTTATGGTCATGGTTTCGTTTCATCCTCG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0120 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF16ATACCAGCTTATTCAATT-CCCGCGATATGACCATCCAGCTGCTCCTCTGTTATTGTTGGTTTTCCTGTTTTCCTTTGT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0121 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF17ATACCAGCTTATTCAATT-CCCCAGTGCTCAGCTTCTCCTCCGTCTCATTCTTCTGTAGGATCGTTCTGTTTTGGTACT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow Cytometry57.63 ± 11.64 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0122 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF10ATACCAGCTTATTCAATT-CCCACCGTCCTCGTGCTTAGTCTTTATGTTTCGTCCTTTTCTTTCTTCGTTCTGTCGCCT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0124 223100302012Development of RNA aptamers for detection of Salmonella EnteritidisS25GGGUUCACUGCAGACUUGACGAAGCUU-GAGAGAUGCCCCCUGAUGTGCAUUCUUGUUGUGUUGCGGC-AAUGGAUCCACAUCTACGAAUUC905'-GGGUUCACUGCAGACUUGACGAAGCUU-N40-AAUGGAUCCACAUCUACGAAUUC-3'ssRNASalmonella Enteritidis (S. Enteritidis)Whole cellIdentify an aptamer that binds to the outer membrane protein C and specifically recognizes S. enteritidis without any cross-reactivity to other Salmonella serovars.Fluorescence intensity of S. Enteritidis enhanced as the concentration of the aptamer S25 increased from 5 to 30 μg.10Flow CytometryN/ADetectionMagnetic Bead (MB)-based SELEX5'-Fluoro-labeled with Fluorescein-maleimideN/AN/AN/AN/AN/A
ABdb_0125 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA3PATAGGAGTCACGACGACCAGAA-TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.Showed a high binding affinity of 76% for V. parahemolyticus and a low apparent binding affinity of 4% for other bacteria.9Flow Cytometry16.88 ± 1.92DetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0126 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA1TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG405'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A1 was 64%.9Flow Cytometry22.92 ± 4.72 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0127 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA1PATAGGAGTCACGACGACCAGAA-TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A1P was 67%.9Flow Cytometry21.45 ± 2.62 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0128 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA3TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT405'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A3 was 75%.9Flow Cytometry24.03 ± 5.18 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0129 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA17PATAGGAGTCACGACGACCAGAA-AGGCCGGCCCCTCTGAACTAGATGCAGGGGAGGCGAGCGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0130 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA18PATAGGAGTCACGACGACCAGAA-GCGGGCAGCTCCACCGGTAGGCTCCGAGTCATCACGGTCG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A18P was 62%.9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0131 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA21PATAGGAGTCACGACGACCAGAA-TTTTGACGAAGTCAGTGCGTCGAAGCGCAAGCATTACAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0132 224389272012A single-stranded DNA aptamer that selectively binds to Staphylococcus aureus enterotoxin BAPT(SEB1)GGTATTGAGGGTCGCATC-CACTGGTCGTTGTTGTCTGTTGTCTGTTATGTTGTTTCGT-GATGGCTCTAACTCTCCTCT785'-GGTATTGAGGGTCGCATC-N40-GATGGCTCTAACTCTCCTCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to SEB and quantification of all of the SEs in S. aureus contaminated food.Have an affinity and high selectivity for SEB.14N/AN/ADetectionSELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0133 224389272012A single-stranded DNA aptamer that selectively binds to Staphylococcus aureus enterotoxin BAPT(SEB2)GGTATTGAGGGTCGCATC-CCGTAGTGTGTTCTTATTCGTGTCTGTGTGTGTTCTGTCG-GATGGCTCTAACTCTCCTCT785'-GGTATTGAGGGTCGCATC-N40-GATGGCTCTAACTCTCCTCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to SEB and quantification of all of the SEs in S. aureus contaminated food.N/A14N/AN/ADetectionSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0134 222189722012Development of aptamer beacons for rapid presumptive detection of Bacillus sporesBAS-6FATACGGGAGCCAACACCATCCCTCTTAGGATACAAAGCCAAACTGAGCCCGTGCAGAGCAGGTGTGACGGAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNABacillus Anthracis (BA) (nonpathogenic Sterne strain)Anthrose and Whole B. anthracis sporesAptamer beacon that specifically binds to the Bacillus spores.Limit of detection (LOD) of approximately 30,000 to 60,000 B. anthracis spores per ml.5N/AN/ABiosensorMagnetic Bead (MB)-based SELEX5'-TYE 665 dye and 3'-Iowa Black end labelsN/AN/AN/AN/AN/A
ABdb_0135 222189722012Development of aptamer beacons for rapid presumptive detection of Bacillus sporesBAS-6RATCCGTCACACCTGCTCT-GCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGGA-TGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNABacillus Anthracis (BA) (nonpathogenic Sterne strain)Anthrose and Whole B. anthracis sporesAptamer beacon that specifically binds to the Bacillus spores.Limit of detection (LOD) of approximately 30,000 to 60,000 B. anthracis spores per ml.5N/AN/ABiosensorMagnetic Bead (MB)-based SELEX5'-TYE 665 dye and 3'-Iowa Black end labelsN/AN/AN/AN/AN/A
ABdb_0138 225206542012Evaluation of the clinical value of ELISA based on MPT64 antibody aptamer for serological diagnosis of pulmonary tuberculosisMPT64-A1GGGAGCTCAGAATAAACGCTCAAA-GGGAGCTCAGAATAAACGCTCAAAAACGCTCAAGAGGCCCGGATCTTCGACATGAGGCCCGGATC-CGACATGAGGCCCGGATC1075'-GGGAGCTCAGAATAAACGCTCAAA-N35-CGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv)MPT64 antibodyIdentify aptamers against MPT64 antibody and develop a sandwich ELISA for the serological diagnosis of pulmonary TB (PTB).LOD was 2.5 mg/L, with a linear range varying from 10 mg/L to 800 mg/L.12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0147 223702802012Harnessing aptamers for electrochemical detection of endotoxinB1CTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCA-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.Achieved a linear detection range of 0.01 to 1 ng/mL of LPS with a detection time of less than 15 minutes.3Surface Plasmon Resonance (SPR)20 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0148 223702802012Harnessing aptamers for electrochemical detection of endotoxinB2CTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)12 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0149 223702802012Harnessing aptamers for electrochemical detection of endotoxinB3CTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGTTGGCCAGATGATATAAAGGGTCAACCCCCCA-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)32 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0150 223702802012Harnessing aptamers for electrochemical detection of endotoxinB4CTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCG-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)21 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0151 223702802012Harnessing aptamers for electrochemical detection of endotoxinB5CTTCTGCCCGCCTCCTTCC-CTAAGCACAGGGAAACCAGCTAATGAGTTAGGCCTGTCCCCCACG-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)484 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0152 223702802012Harnessing aptamers for electrochemical detection of endotoxinB6CTTCTGCCCGCCTCCTTCC-CAATGGACCTATTCGAGTACTGAATAGAACAGTCGGCGCTCTGGG-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)298 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0153 223702802012Harnessing aptamers for electrochemical detection of endotoxinB7CTTCTGCCCGCCTCCTTCC-TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)61 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0154 223702802012Harnessing aptamers for electrochemical detection of endotoxinB8CTTCTGCCCGCCTCCTTCC-ATCCAATACCTCAGAACTCAGTTCGAGTCGTAAAGGGGAATCGCA-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)71 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0155 223702802012Harnessing aptamers for electrochemical detection of endotoxinB9CTTCTGCCCGCCTCCTTCC-ACCGATCCATCGAGTTTCTGAGAAAGGCCCGGAGAAACCGCGAGA-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)15 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0156 223702802012Harnessing aptamers for electrochemical detection of endotoxinB10CTTCTGCCCGCCTCCTTCC-TCAATCTAACCATGCATGCAGTTTAGGCAGGATTCGTTATCGCAA-GGAGACGAGATAGGCGGACACT865'-CTTCTGCCCGCCTCCTTCC-45N-GGAGACGAGATAGGCGGACACT-3'ssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Lipopolysaccharide (LPS)Identify aptamers showing specific affinity to LPS and develop an electrochemical aptasensor on a gold surface for its detection.N/A3Surface Plasmon Resonance (SPR)2336 nMBiosensorNECEEM-based non-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0157 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-1CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTG465'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0158 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-2ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCAT405'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0159 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-3GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGC395'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).Successfully detect S. typhimurium down to 600 CFU/mL (equivalent to 18 live cells in 30 μL of assay volume) and distinguish it from other Salmonella species, including S. enteritidis and S. choleraesuis.12Flow Cytometry25 nMDetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0160 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-4CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0161 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-5GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTCGCATAGGTAGTCCAGAAGCC615'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0162 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-6CTCCTCTGACTGTAACCACG-GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGCC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0163 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-7CTCCTCTGACTGTAACCACG-GTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGTC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0164 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-8CTCCTCTGACTGTAACCACG-GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0165 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-9CTCCTCTGACTGTAACCACG-ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCATC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0166 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8E06TGATCCGGGCCTCATGTCGAACCCACACCCCACAACCACCCAGCCCCAGCCCGCTATTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0167 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8F04TGATCCGGGCCTCATGTCGAACACAACACCCAGCCAACGACCACAACTCCAACTCATTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0168 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8C10TGATCCGGGCCTCATGTCGAAACCACAACCCAACAACAAGAACCACAAAGAGCCCCTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0169 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8B06TGATCCGGGCCTCATGTCGAACACACACAGAACCACAACCACTAAACACGAGGGCCTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0170 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8B10GATCCGGGCCTCATGTCGAACACCCCCCAACTAAAACAACAAAACACCACCGCCATTGAGCGTTTATTCTGAGCTCCCA795'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.Digoxigenin-B10 bound to the Salmonella O8 target with high specificity, producing a clear fluorescent signal within 1.5–2 h.11Aptamer-Linked Immobilized Sorbent Assay (ALISA)32.04 nMDetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0171 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8C04TGATCCGGGCCTCATGTCGAACCAACAAGGCAGAAAGAAACCGACAAACCGCACTGTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0172 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8F05TGATCCGGGCCTCATGTCGAACCAACAAGGCAGAAAGAAACCGACAAACCGCACTGTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0173 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8B05TGATCCGGGCCTCATGTCGAACCGAACGACTCAAAGATCAAGCCAAGCCACGCCCGTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0174 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8G05TGATCCGGGCCTCATGTCGAACCAACCCAACACCAAAGAGACCACCACCACACGAGTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)175.9 nMDetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0175 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8D04TGATCCGGGCCTCATGTCGAACACGAGCCACCAACGCACCAAAACCCGTCCTCCACTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0176 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8E04TGATCCGGGCCTCATGTCGAAGGCACGCGCACCAAAACACAAACTCCCCCCGCACCTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0177 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8G06TGATCCGGGCCTCATGTCGAAGGGCCACCTAACCACCTCTGCCAATACCCCCCGCGTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0178 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8C05TGATCCGGGCCTCATGTCGAAGGGAAGTCATGGCATGGATGCGCTCCCCGCCCACCTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0179 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8C06TGATCCGGGCCTCATGTCGAAGGGAAGTCATGGCATGGATGCGCTCCCCGCCCACCTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0180 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8H05TGATCCGGGCCTCATGTCGAACGGCGCAGTGACACGGTAGGGAGTCGTGCCGCGGCTTGAGCGTTTATTCTGAGCTCCCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0181 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8H06TGGGAGCTCAGAATAAACGCTCAAGGTGGGCGGGCGGCGTTGCTGTTCTTTTGCTGGTGTTCGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0182 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8F06TGGGAGCTCAGAATAAACGCTCAAGAGGCGGCCTGTTCGCTTGTTGTGGGTCCGCTTGGTTCGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0183 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8A05TGGGAGCTCAGAATAAACGCTCAAGGGCACTGGGTGGTGATGTTTTGTTTGTCTGGCGGTTCGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0184 https://doi.org/10.1007/s00604-012-0825-22012Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8D06TGGGAGCTCAGAATAAACGCTCAAGGGGGGGGTGGAGGGGTATGCAGTTTCGGTGGCCGTTCGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNASalmonella O8Whole cellIdentified aptamers for the detection of Salmonella O8.N/A11Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0185 222263272012Bio-capture of S. Typhimurium from surface water by aptamer for culture-free quantificationSal aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-based bio-capture assay for the detection of selective serovar species through molecular-beacon-based real-time PCR.Achieved a detection limit of 1 CFU/PCR or 100 CFU/ml.N/AN/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0187 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-1CTCCTCTGACTGTAACCACG-CACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0188 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-2CTCCTCTGACTGTAACCACG-TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACT-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0189 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-3CTCCTCTGACTGTAACCACG-TATACGCGCTTGCCCCTTAGTCATACGAACTGATTCAATC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0190 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-4CTCCTCTGACTGTAACCACG-TGCCGCTAAACGCCGGCTCATCGTTATGCTTTTCATTGCA-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0191 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-5CTCCTCTGACTGTAACCACG-TAACGTACCAAAATGTTGGATTGGATGTTGTACTGGGTT-GCATAGGTAGTCCAGAAGCC795'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0192 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-6TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCC605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0193 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-7CTCCTCTGACTGTAACCACGTATACGCGCTTGCCCCTTAGTCATACGAACTGATTCAATC605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0194 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-8CTCCTCTGACTGTAACCACGAACGATTCAAGAACTGTTGGTTGTCGGCTTATTTTCGCCA605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0195 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-9CTCCTCTGACTGTAACCACGCACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).Can successfully detect S. enteritidis down to 600 CFU/mL (equivalent to 18 CFU in 30 μL assay volume) in 10 min and distinguish it from other Salmonella species, including S. typhimurium and S. choleraesuis.12N/AN/ABiosensorWhole Cell-SELEX5'-6-hydroxyhexyl disulfide group (5'-/5ThioMC6)N/AN/ABest CandidateN/AN/A
ABdb_0196 229711462012Aptamer-based impedimetric sensor for bacterial typingSENT-10TACCAAAATGTTGGATTGGATGTTGTACTGGGTTGCATAGGTAGTCCAGAAGCC545'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellDevelopment of an aptamer-based impedimetric sensor for the typing of bacteria (AIST-B).N/A12N/AN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0197 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-3 (80nt)CTCCTCTGACTGTAACCACG-TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACT-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0198 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-3 (60nt)TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCC605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry7.8 ± 6.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0199 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-6 (79nt)CTCCTCTGACTGTAACCACG-TAACGTACCAAAATGTTGGATTGGATGTTGTACTGGGTT-GCATAGGTAGTCCAGAAGCC795'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry53 ± 7 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0200 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-6_54TACCAAAATGTTGGATTGGATGTTGTACTGGGTTGCATAGGTAGTCCAGAAGCC545'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry6.3 ± 0.58 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0201 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-20_80CTCCTCTGACTGTAACCACG-CACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry28 ± 3.8 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0202 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-20_60CTCCTCTGACTGTAACCACGCACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis and 43 ± 4% (SE-20, 60nt) in S. enteritidis.12Flow Cytometry7.1 ± 0.62 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0203 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-22_80CTCCTCTGACTGTAACCACG-TATACGCGCTTGCCCCTTAGTCATACGAACTGATTCAATC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry30 ± 4.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0204 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-22_60CTCCTCTGACTGTAACCACGTATACGCGCTTGCCCCTTAGTCATACGAACTGATTCAATC605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry5.3 ± 0.7 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0205 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-11_60CTCCTCTGACTGTAACCACGAACGATTCAAGAACTGTTGGTTGTCGGCTTATTTTCGCCA605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry6.9 ± 0.4 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0206 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-34_80CTCCTCTGACTGTAACCACG-TGCCGCTAAACGCCGGCTCATCGTTATGCTTTTCATTGCA-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry56 ± 7.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0207 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-1_40GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGC395'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0208 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-6_60GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTCGCATAGGTAGTCCAGAAGCC615'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0209 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-12_80CTCCTCTGACTGTAACCACG-GTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGTC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0210 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-12_60CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT595'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium and 62 ± 12% (ST-12, 60nt) in S. typhimurium.12Flow Cytometry4.5 ± 0.4 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0211 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-20_80CTCCTCTGACTGTAACCACG-ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCATC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0212 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-20_40ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCAT405'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0213 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-33_60CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow Cytometry51.0 ± 4.3 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0214 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST2PATAGGAGTCACGACGACCAGAA-AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.The limit of detection (LOD) was 25 cfu/mL, and the percentage gated fluorescence intensity above library background was 20% when ST9P was incubated with L. monocytogenes cells and 72% when incubated with S. typhimurium.9Flow Cytometry6.33 ± 0.58 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0215 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST2AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA405'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 82 ± 2.34% for ST2.9Flow Cytometry16.34 ± 0.45 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0216 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST3PATAGGAGTCACGACGACCAGAA-TTTCGGCTAAGGACGGGTGAAATACATTTAATAGGGTGGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.N/A9Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0217 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST7PATAGGAGTCACGACGACCAGAA-TAAGTACAGGACTGGAGTATTAGCGGGGTCCATGCAAGGC-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.N/A9Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0218 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST9AATCAATAGAAGACAAAGTCCGAAACAGGTGTGACGGTAA405'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 72 ± 1.57% for ST9.9Flow Cytometry9.45 ± 0.63 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0219 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST9PATAGGAGTCACGACGACCAGAA-AATCAATAGAAGACAAAGTCCGAAACAGGTGTGACGGTAA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 66 ± 1.45% for ST9P.9Flow Cytometry11.14 ± 0.12 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0220 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC4CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGGCGTGGGGGCAATGCCTGCTTGTAGGCTTCCCCTGTGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.C4 showed strong specific binding to S. Typhimurium, and the increase in the fluorescence intensity of C4 was less than 30% against E. coli and S. aureus.10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0221 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC1CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGTCGGAGCCAGGATGCGAGGTCTGTAGGTCTGCGGGCGG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0222 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGCGGGCGAGTTGACGGCGTAATCGTGCTGCCGCGTG-GGTGGATCCATATTCCTACTCG995'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0223 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGGGCGTGGCTCAGAGTGGGGGTCGGTCAGTCTTGTGCGCG-GGTGGATCCATATTCCTACTCG1025'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0224 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC5CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGACGCCTGCGTGGCTGAGGCTTCGGTCTGCGCCG-GGTGGATCCATATTCCTACTCG1015'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0225 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC6CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CGTGCGGCGGGCAGGATGGGATGCTGTAGGTCTGCGGGCGCGCG-GGTGGATCCATATTCCTACTCG1045'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0226 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC7CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CGTGCGGAGCCAGGATGGGAGGTCTGTAGGTCTGCGGGGCGCG-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0227 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC8CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGGGACGGGTACCGGGCGTGTGGCGTGCCTGCCGCG-GGTGGATCCATATTCCTACTCG995'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0228 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC9CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGTGCGGGCCGGATGGGAGCTCTGTAGGTTGCGGGCGCGG-GGTGGATCCATATTCCTACTCG1015'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0229 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA1/SA-1CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGGGGCCAGGATGCGA-GGTGGATCCATATTCCTACTCG825'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0230 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA2/SA-2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GGTCTGTAGGTCTGCGGCGCGCG-GGTGGATCCATATTCCTACTCG835'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0231 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA3/SA-3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGGGGCCAGGATGCGAGGTCTGTAGGTCTGCGGCGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0232 236982752013Highly specific and cost-efficient detection of Salmonella Paratyphi A combining aptamers with single-walled carbon nanotubesApt22GAATTCAGTCGGACAGCG-ATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGCG-GATGGACGAATATCGTCTCCC805'-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3'ssDNASalmonella Paratyphi AWhole cellIdentify aptamers that bind to and detect S. Paratyphi A based on the noncovalent self-assembly of individual SWNTs and DNAzyme-labelled aptamer detection probe.LOD of the method was 10(3) cfu/mL with a range from 10(3) to 10(7) cfu/mL, and detection signals of target bacteria were 4.4-fold higher than S. Enteritidis, 4.3-fold higher than S. Arizonae, 56.3-fold higher than S. aureus, and 24.3-fold higher than E. coli K88.17Fluorescence Spectroscopy47 ± 3 nMBiosensorWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0233 236982752013Highly specific and cost-efficient detection of Salmonella Paratyphi A combining aptamers with single-walled carbon nanotubesApt10GAATTCAGTCGGACAGCG-GATGATGGACGTATATCGTCTCCCATGAATTCAGTCGGACAGCG-GATGGACGAATATCGTCTCCC835'-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3'ssDNASalmonella Paratyphi AWhole cellIdentify aptamers that bind to and detect S. Paratyphi A based on the noncovalent self-assembly of individual SWNTs and DNAzyme-labelled aptamer detection probe.N/A17Fluorescence Spectroscopy73 ± 9 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0234 236982752013Highly specific and cost-efficient detection of Salmonella Paratyphi A combining aptamers with single-walled carbon nanotubesApt 45GAATTCAGTCGGACAGCG-ATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGC-GATGGACGAATATCGTCTCCC795'-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3'ssDNASalmonella Paratyphi AWhole cellIdentify aptamers that bind to and detect S. Paratyphi A based on the noncovalent self-assembly of individual SWNTs and DNAzyme-labelled aptamer detection probe.N/A17Fluorescence Spectroscopy68 ± 6 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0235 236982752013Highly specific and cost-efficient detection of Salmonella Paratyphi A combining aptamers with single-walled carbon nanotubesApt 60GAATTCAGTCGGACAGCG-CGCCCACCCATAATGGATCAGGGCGGGCACCACGATG-GATGGACGAATATCGTCTCCC765'-GAATTCAGTCGGACAGCG-N40-GATGGACGAATATCGTCTCCC-3'ssDNASalmonella Paratyphi AWhole cellIdentify aptamers that bind to and detect S. Paratyphi A based on the noncovalent self-assembly of individual SWNTs and DNAzyme-labelled aptamer detection probe.N/A17Fluorescence Spectroscopy56 ± 9 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0236 236895052013Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticlesSA17TCCCTACGGCGCTAAC-CCCCCCAGTCCGTCCTCCCAGCCTCACACC-GCCACCGTGCTACAAC625'-TCCCTACGGCGCTAAC-N30-GCCACCGTGCTACAAC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC: 6538DR)Whole cellIdentify aptamers that binds to and recognize strains of S.aureus using aptamer-conjugated gold nanoparticles.LOD was 312 bacterial cells, and the lower limit of detection of the instrument was 63 ± 21 GNPs/μl for 100-nm particles and 508 ± 176 GNPs/μl for 60-nm particles.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)35 nM and 3.03 nM for SA17-GNPsBiosensorWhole Cell-SELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0237 236895052013Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticlesSA61TCCCTACGGCGCTAAC-CTCCCAACCGCTCCACCCTGCCTCCGCCTC-GCCACCGTGCTACAAC625'-TCCCTACGGCGCTAAC-N30-GCCACCGTGCTACAAC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC: 6538DR)Whole cellIdentify aptamers that binds to and recognize strains of S.aureus using aptamer-conjugated gold nanoparticles.LOD was 1250 bacterial cells, and the lower limit of detection of the instrument was 63 ± 21 GNPs/μl for 100-nm particles and 508 ± 176 GNPs/μl for 60-nm particles.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)129 nM and 9.9 nM for SA61-GNPsBiosensorWhole Cell-SELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0238 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA1ATCCAGACGTGACGCAGC-ATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTG-TGGACACGGTGGCTTAGTA755'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA1 and DTMRSA3 showed the best specificity for MRSA.17Flow Cytometry1.6 ± 0.5 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/ABest CandidateN/AN/A
ABdb_0239 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA2ATCCAGAGTGACGCAGCA-CGACACGTTAGGTTGGTTAGGTTGGTTAGTTTCTTG-TGGACACGGTGGCTTA705'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA2 and DTMRSA4 bound to all three types of clinical bacterial strains.17Flow Cytometry2.0 ± 0.6 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0240 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA3ATCCAGAGTGACGCAGCA-GTAGATGGTTTGGTTGGTGTGGTTTCCTACTGATGTTGGG-TGGACACGGTGGCTTAGTA775'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA1 and DTMRSA3 showed the best specificity for MRSA.17Flow Cytometry1.3 ± 0.5 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0241 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA4ATCCAGAGTGACGCAGCA-TTATGGGGTGTGGTGGGGGGTTAATGCGTTGGTTATCCG-TGTGGACACGGTGGCTTA755'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA2 and DTMRSA4 bound to all three types of clinical bacterial strains.17Flow Cytometry9.5 ± 2 × 10(1) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0242 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 1GCAATGGTACGGTACTTCC-CAACCCCGTCTATCACGTCGCTCTTGCGTTGGTTG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)7.5 ~ 10 nM (for ALS)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0243 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 2GCAATGGTACGGTACTTCC-CAACCCCGTCTATCACGTCGCTGTTGCGTTGGTTG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)7.5 ~ 10 nM (for ALS)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0244 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 3GCAATGGTACGGTACTTCC-CGCCGCCGCGTTCTCATCGCGTGATGTAGTGTCCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)17.5 ~ 20 nM (for ALS) and 30 ~ 35 nM (for 3O-C12-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0245 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 4GCAATGGTACGGTACTTCC-CGCCGGCTTCTCTTGCCGTGATGTAGTGTCCG-CAAAAGTGCACGCTACTTTGCTAA755'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)15 nM (for ALS) and 20 ~ 25 nM (for 3O-C12-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0246 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 5GCAATGGTACGGTACTTCC-CGGGGCCCGCTTCTGGTGCGGTGTACTAGTGACCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.ALSap-5 at 0.5 µM, about 90% of biofilm, 50.9% reduced pyocyanin secretions, as well as secretions of LasA protease and LasB elastase were inhibited.14Enzyme-Linked Immunosorbent Assay (ELISA)10 ~ 12.5 nM (for ALS) and 20 nM (for 3O-C12-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/ABest CandidateN/AN/A
ABdb_0247 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 6GCAATGGTACGGTACTTCC-CGGGGCGGGCTGTCATGCCCATCCTACCGTGACCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)15 ~ 17.5 nM (for ALS) and 45 ~ 50 nM (for C4-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0248 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 7GCAATGGTACGGTACTTCC-CGGGGCGGCCTGTGTTGGCCTACCTAGCGAGACCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.N/A14Enzyme-Linked Immunosorbent Assay (ELISA)12.5 ~ 15 nM (for ALS) and 35 ~ 40 nM (for C4-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/AN/AN/AN/A
ABdb_0249 https://doi.org/10.1007/s12257-012-0556-62013Screening and anti-virulent study of N-acyl homoserine lactones DNA aptamers against Pseudomonas aeruginosa quorum sensingALSap 8GCAATGGTACGGTACTTCC-CGCTGCCCCCTGTCCTGGGTTAGCTAGCGAGAGCG-CAAAAGTGCACGCTACTTTGCTAA785'-GCAATGGTACGGTACTTCC-N35-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAPseudomonas Aeruginosa PAO1Amino lactam surrogate (ALS) of N-acyl homoserine lactone (HSL)DNA aptamers that bind specifically to HSL and show strong inhibitory activity on biofilm formation.86% of pyocyanin secretion was inhibited by 6 µM of ALSap-8, and biofilm formation, and the secretions of LasA protease and LasB elastase were also decreased by 9.3%, 17.5%, and 19% respectively.14Enzyme-Linked Immunosorbent Assay (ELISA)10 nM (for ALS) and 25 ~ 30 nM (for C4-HSL)TherapeuticsSELEXN/AAptamers had no influence on bacterial growth.N/ABest CandidateN/AN/A
ABdb_0250 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-17AGTATACGTATTACCTGCAGC-GAGGGAAGAAGGGCCAGCACAGATCAGATCAATCGCTCCG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.Lbi-17 showed maximum binding of 78.7 ± 12.38% with an LOD of the combined AMC-qPCR method of 1.8 log10 CFU/500μl L.monocytogenes and ranged from 26% to 77%.6Flow Cytometry35.7 ± 8.02 μMBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0251 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-16AGTATACGTATTACCTGCAGC-AAATACTTTAGATCTAAGAGTGTTTCGAAAAGACAACAGA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0252 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-118AGTATACGTATTACCTGCAGC-TTGAATTAATAGATTAGTATACTTGGGAATCGTCCTAATA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0253 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-200AGTATACGTATTACCTGCAGC-AGGAAGACAAATTCCGCCAAAAAGTGGATATAACCAATAA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0254 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-203AGTATACGTATTACCTGCAGC-ATAGAGTAGAAGCTACACTACGTAATCACAGACAGATCCA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0255 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA15GGGAGCTCAGAATAAACGCTCAA-TACTATCGCGGAGACAGCGCGGGAGGCACCGGGGA-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.Display a LOD of 75 CFU/mL and a wide linear range from 10(2) to 10(7).8Fluorescence Spectroscopy48.74 ± 3.11 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0256 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA1GGGAGCTCAGAATAAACGCTCAA-GGGGGGCCTAGACTAGGGGGAGAGGGTGGGACGGT-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy86.41 ± 3.34 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0257 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA10GGGAGCTCAGAATAAACGCTCAA-GGGGCGGCGGCGGTGGTACGGGGTTGGGAGCGGGC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy203.12 ± 1.86 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0258 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA9GGGAGCTCAGAATAAACGCTCAA-GGCGTATGCGCAGCGAGGGCGGCCGGGCGACGTCG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy88.36 ± 3.60 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0259 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA8GGGAGCTCAGAATAAACGCTCAA-CCACGGGAACAACATCGTGGCAGGGACGAGCGTCCT-TTCGACATGAGGCCCGGATC795'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy120.91 ± 2.85 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0260 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA4GGGAGCTCAGAATAAACGCTCAA-GCGCGCTGCCGACGCGGGGGGGCTGATTAGCGTGG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy205.83 ± 1.65 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0261 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA13GGGAGCTCAGAATAAACGCTCAA-ACTGAGGGGCGGCGGACGGGATGGGAAATGTAGG-TTCGACATGAGGCCCGGATC775'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy299.94 ± 1.67 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0262 https:doi.org10.1016j.foodcont.2013.03.0112013Selection, identification and application of a DNA aptamer against Listeria monocytogenesA12GGGAGCTCAGAATAAACGCTCAA-TAGCGTGGGTAACCGTGTTGGGGGGTGCCACGGTC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to recognise Listeria monocytogenes.N/A8Fluorescence Spectroscopy63.41 ± 3.39 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0263 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA1AGCAGCACAGAGGTCAGATG-AGGCGATTACGCTTCTTGTACTTCAATAACGACTCAACTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy92.17 ± 16.75 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0264 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA15/40AGCAGCACAGAGGTCAGATG-TACTTATGCATTTCCTCCCACGATCTTATTTGAGAGTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.Limit of detection of 8.7±10(-3) μg/mL with linear range of 0.01-10 μg/mL SEA.12Fluorescence Spectroscopy48.57 ± 6.52 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0265 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.2AGCAGCACAGAGGTCAGATG-ATGATCGTAGTCATTTAAAATTTGAATACTATCAAAGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy235.00 ± 79.05 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0266 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA3AGCAGCACAGAGGTCAGATG-TACGAGCGGTGGTTTTACCCTGCAATACTTTTGGCTGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0267 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA25AGCAGCACAGAGGTCAGATG-ATAGATTTTTTTCATGATTCTTCATTTTTTTATTTGAAAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0268 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA29AGCAGCACAGAGGTCAGATG-TATTATCTTACTACGTTGCATCCTACTTTTATAGTCCCCT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0269 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA30AGCAGCACAGAGGTCAGATG-GGATCTTCCCTCAATGTTTATTGTATATCTGTACTCGTAA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0270 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA10AGCAGCACAGAGGTCAGATG-TCCAATTCAATTCATTTCTGAACTTAGTCGGCACTTTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0271 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA4AGCAGCACAGAGGTCAGATG-CTCCAGCAGCCTCATAGATACGTCGTATCTATTGTTTCCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0272 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.1AGCAGCACAGAGGTCAGATG-AAGGTCTTACATCAATTTATATATTTTTCCAATATCTGTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0273 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2GGGAGAGCGGAAGCGUGCUGGG-UAGUGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGA-CAUAACCCAGAGGUCGAUGGUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20.27 ± 4.372 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0274 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2 Truncated 43-merGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGAC395'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)27.64 ± 4.784 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0275 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #2GGGAGAGCGGAAGCGUGCUGGGCC-GGGAGUUUUGAUACGGCUUCAUGCAGUAAUGUUUUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.The level of binding of #2 RNA aptamer to S. aureus was 1.6-fold higher than that of the control aptamer.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/ABest CandidateN/AN/A
ABdb_0276 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #10GGGAGAGCGGAAGCGUGCUGGGCC-GUUAAUACGGUGUCUUUUUCGGUCGUGUAUAAAACGGAAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0277 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #14GGGAGAGCGGAAGCGUGCUGGGCC-UAGGACAGUUCGUCCUCAUUACAUCGCCGCCUAACACAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0278 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #16GGGAGAGCGGAAGCGUGCUGGGCC-UUAGAAGUAGCCUGCUACGCAUGGUCGACUCAAGAAUCG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0279 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #18GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0280 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #21GGGAGAGCGGAAGCGUGCUGGGCC-AGUCUGACACGUAGACAGUUCUAUUACUUACGUCGAGA-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0281 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #23GGGAGAGCGGAAGCGUGCUGGGCC-ACAGUGUUCUAAUGCGACAAUGGAGUCUGUGGCAAAGUGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0282 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #24GGGAGAGCGGAAGCGUGCUGGGCC-UCUCAGGCCGACAUUCUGAGAACGCGAGGCGUAUUGAAG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0283 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #26GGGAGAGCGGAAGCGUGCUGGGCC-UUCAAGUAGGGGCGGUUUACUAUCUGGAUCUUGUAGUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0284 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #07GGGAGAGCGGAAGCGUGCUGGGCC-ACUUGGGGACGACGAGUAGAUAGUAAGGUGGAGACCUGGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0285 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #06GGGAGAGCGGAAGCGUGCUGGGCC-UAUGACAUAAGGUGGGCUGGGAAGCUAGAGCAUGUAAGG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0286 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #05GGGAGAGCGGAAGCGUGCUGGGCC-GUGAAGAAAAAGGGGCGGAUUGGGUAGUAGGGAGGAGAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0287 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #04GGGAGAGCGGAAGCGUGCUGGGCC-AGGAUAGGGGAUGAAGAAAAAAAGAAGGGUGCCGUGGCGC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0288 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone G1GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0289 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA101ATTACTTACGCTATCTAAttt-GGGGGGTGGGTTGTTTGGGATGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0290 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA102ATTACTTACGCTATCTAAttt-GGGGGGGGAACATGTTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting Assay3.5 nM (for B4) and 1.4 nM (for DL1d-A6)DetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0291 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA103ATTACTTACGCTATCTAAttt-GGGGCGGGACTTATTTGGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0292 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA104ATTACTTACGCTATCTAAttt-GGGGGTGGGTGCTTTTGTGGTGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0293 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA105ATTACTTACGCTATCTAAttt-TAGGGGTGGGTTCAATTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0294 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA106ATTACTTACGCTATCTAAttt-GGGGGGCGGGTATTAATGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0295 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA107ATTACTTACGCTATCTAAttt-GTTTTCGTGGGGGGGTGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0296 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA108ATTACTTACGCTATCTAAttt-GCACTAAAGGGGAGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0297 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA109ATTACTTACGCTATCTAAttt-TTGCTTTAGGGGAGGTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting Assay7.7 nM (for B4) and 4.1 nM (for DL1d-A6)DetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0298 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA110ATTACTTACGCTATCTAAttt-TCGCTGATGGGGAGGAGGGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0299 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA111ATTACTTACGCTATCTAAttt-GCCCGATGGGGGTGGCGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0300 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G02ATTACTTACGCTATCTAAttt-TTGCTGTAGGGGAGGAGGGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Displayed the highest specificity, exhibiting a 36% higher specificity index than that of the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0301 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G19ATTACTTACGCTATCTAAttt-TTTTTCGGGGGGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0302 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G14ATTACTTACGCTATCTAAttt-TTGCTTTAGAGGAGGCGGGTGGAG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0303 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G11ATTACTTACGCTATCTAAttt-TCGGTGATGGGGAGGAGGCGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0304 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G03ATTACTTACGCTATCTAAttt-GTTTTATGGGGGTGGCGTGTGGCG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0305 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G06ATTACTTACGCTATCTAAttt-TCGCTGATGGGGAGGAGCGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0306 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G10ATTACTTACGCTATCTAAttt-TTACTTTTGGGGGGGCGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0307 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G02ATTACTTACGCTATCTAAttt-GCACGTTTGGGGAGGTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0308 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G09ATTACTTACGCTATCTAAttt-TTTTTCGAGGGGAGATTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0309 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G20ATTACTTACGCTATCTAAttt-GCGCGAGTGGGGGGGTGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0310 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G04ATTACTTACGCTATCTAAttt-GCCCGCGTCGGGGGGTGGGGGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0311 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G01ATTACTTACGCTATCTAAttt-TCGCTATGGGGGTGGCGGCTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0312 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G17ATTACTTACGCTATCTAAttt-TAGCTTTAGGGGTCGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0313 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G08ATTACTTACGCTATCTAAttt-GAGCTTTAGAGGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0314 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G04ATTACTTACGCTATCTAAttt-TTCCTTGGGGAGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0315 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G06ATTACTTACGCTATCTAAttt-GTTTTCGGGGGCTGGTGGTTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0316 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G09ATTACTTACGCTATCTAAttt-TGGCTCGGGGGGTGTTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0317 233572352013Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approachE1GCAATGGTACGGTACTTCC-ACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCG-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolated and characterized aptamers against Escherichia coli.Display binding ranging from 42.6% to 131.8% to E. coli.10Fluorescence Spectroscopy12.4 nMDetectionWhole Cell-SELEX3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0318 233572352013Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approachE2GCAATGGTACGGTACTTCC-CCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCG-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolated and characterized aptamers against Escherichia coli.Display binding ranging from 42.6% to 131.8% to E. coli.10Fluorescence Spectroscopy25.2 nMDetectionWhole Cell-SELEX3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0319 233572352013Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approachE10GCAATGGTACGGTACTTCC-GTTGCACTGTGCGGCCGAGCTGCCCCCTGGTTTGTGAATACCCTGGG-CAAAAGTGCACGCTACTTTGCTAA905'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolated and characterized aptamers against Escherichia coli.Display binding ranging from 42.6% to 131.8% to E. coli.10Fluorescence Spectroscopy14.2 nMDetectionWhole Cell-SELEX3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0320 233572352013Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approachE12GCAATGGTACGGTACTTCC-GCGAGGGCCAACGGTGGTTACGTCGCTACGGCGCTACTGGTTGAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolated and characterized aptamers against Escherichia coli.Display binding ranging from 42.6% to 131.8% to E. coli.10Fluorescence Spectroscopy16.8 nMDetectionWhole Cell-SELEX3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0322 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS 1ATAGGAGTCACGACGACCAGAA-CGGAACTAGCGTTTAAATGCCAGGACTGAAGTAGGCAGGG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellIdentify an aptamer targeted against Shigella dysenteriae and develop a sandwich-type fluorescent bioassay for quantification.Obtained linear range between 10(2)-10(7) cfu/mL of S. dysenteriae, and the limit of detection was 50 cfu/mL8Fluorescence Binding Assay23.47 ± 2.48 nMDetectionWhole Cell-SELEX5'-FAM Labeled and BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0323 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS12ATAGGAGTCACGACGACCAGAA-CCTGGCGGGTCCCGGGGTAAACGGCACAAACGATAAAGAA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0324 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS2ATAGGAGTCACGACGACCAGAA-AAGATGACACTTGGCAGCCGCCTCGAGTGTCCTACACGCA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0325 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS8ATAGGAGTCACGACGACCAGAA-GCCAGATGAGGCCGGCAGGGCCCAAGTGTTGCTCGGGCTA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0326 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS21ATAGGAGTCACGACGACCAGAA-TGCACGGGACAAGAGTACACGCCGATTGCCAGGCACAGTG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding Assay75.49 ± 3.74 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0327 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS10ATAGGAGTCACGACGACCAGAA-GGGGAAGCCGATCAGGCCAATCATTGAGGGTGAACTAGCT-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0328 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS19ATAGGAGTCACGACGACCAGAA-TTATCGGCTGGCAAAACTGCGGCTGGAGCTCACAACTAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0329 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS13ATAGGAGTCACGACGACCAGAA-TCAGCGAGGGCCAATTAGAAGGGTACTCATGTCTGTGGAC-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0330 238112062013In vitro selection of a DNA aptamer targeted against Shigella dysenteriaeS24ATAGGAGTCACGACGACCAGAA-CCAGGCGGAATGTGTCTTCGTTTTGCGAGTGTTAAGGGCG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAShigella DysenteriaeWhole cellAptamer S 1 binds to S. dysenteriae with high affinity and specificity. The binding is subsequently used in a sandwich-type fluorescent bioassay for quantificationN/A8Fluorescence Binding AssayN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0331 234946202013Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sortingS8-7GTATACGTATTACCTGCAGC-CTGATGTGTGGGTAGGTGTCGTTGATTTCTTCTGGTGGGG-CGATATCTCGGAGATCTTGC805'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNASalmonella Typhimurium (S. Typhimurium) (Strain S913)Whole cellIdentifying aptamers selected by whole-cell SELEX and developing a qPCR-based capture-detection platform for Salmonella Typhimurium.N/A10Flow Cytometry1.73±0.54 μMDetectionWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0332 234946202013Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sortingS8-46GTATACGTATTACCTGCAGC-CTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG-CGATATCTCGGAGATCTTGC805'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNASalmonella Typhimurium (S. Typhimurium) (Strain S913)Whole cellIdentifying aptamers selected by whole-cell SELEX and developing a qPCR-based capture-detection platform for Salmonella Typhimurium.Achieved a Lower Limit of Detection (LOD) of 10(2)-10(3) CFU in a 290-μl sample, and the mean capture efficiency ranged from 3.6% to 12.6%.10Flow Cytometry0.74±0.20 μMDetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0336 242670762013A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labelsApt1ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellAptamer-modified QDs (QD-apt) were developed to selectively capture and simultaneously detect the target bacteria.For V. parahaemolyticus cells, a linear range of 3.4 × 10(4) to 3.4 × 10(7) cfu/mL was obtained with QD 535-apt 1-FCM, and a detection limit of 5 × 10³ cfu/mL was achieved.N/AN/AN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0337 242670762013A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labelsApt2ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-modified Quantum Dots (QD-apt) were developed for selective bacterial capture.For S. typhimurium, a linear range obtained between the concentrations 3.8 × 10(4) to 3.8 × 10(7) cfu/mL was obtained with QD 585-apt 2-FCM, and a detection limit of 5 × 10³ cfu/mL was achieved.N/AN/AN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0343 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL1GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GGCGCCATAGCGACGGGGCCATTCCAAGAA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).ZXL1 bound to virulent M. tb H37Rv much more strongly (52.2%) than to the other Mycobacteria strains tested, M. smegmatis (2.5%) and BCG (13.9%). LPS-stimulated human DC groups showed that ZXL1 decreased IL-10 production by 31–36% and increased IL-12 production by 52–91%, thereby reducing the progression of infection and bacterial loads in mice and rhesus monkeys.12Enzyme-Linked Oligonucleotide Assay (ELONA)436.3 ± 37.84 nMTherapeutics/ImmunmodulatorySELEXBiotinylated or FAM LabeledZXL1 showed no significant cytotoxicity at concentrations ranging from 2 to 15 microM at the 168-hour test.N/ABest CandidateN/AN/A
ABdb_0344 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL2GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-AGTCCACGATAATCACACACACGGACACCC-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0345 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL4GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CACGCCCAATAGTGGCTCCAGCTCATCTCT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0346 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL5GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CATCGATGTACCCTACCTACATGTTACGTT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0347 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL6GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-ACCTACAGCGACACCTAACTGAGTAGAACT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0348 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL7GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TACCTAACACTTCCTGCTCCACCTTTATTA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0349 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL8GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CCCTGATTCCCCCTCATCCGATGGACATCT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0350 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL9GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GACCCTCATGATACCAACCACTCCAGTCAA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0351 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL10GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TATCCCAGTGGCATTAACTACCCACTCGCA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0352 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL3GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TCACGGAGTGAGGGGCTGTTCATTAAGAGA-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0353 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL12GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TCGCCTGATAGGTCTGTGATCCAATCCAAT-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0354 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL13GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TCTGCTTGCCGCTGATCCGCCAATTCGATC-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0355 245722952014Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeysZXL14GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-TCGTGCCGACGCTGCCGAATAGCACTGCAC-GGGTCAATGCGTCATA885'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentify aptamers that bind to and inhibit ManLAM recognition by host cells and ManLAM-induced immunosuppression of CD11c+ dendritic cells (DCs).N/A12Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0356 251855032014Selection of peptidoglycan-specific aptamers for bacterial cells identificationAntibac1TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC555'-TCGCGCGAGTCGTCTG-N24-CCGCATCGTCCTCCC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923) and Escherichia Coli (E. Coli) TOP10PeptidoglycanIdentify aptamers that bind to the peptidoglycan of bacterial cells.High binding affinity for S. aureus, as the radioactivity of Antibac1 was 28 times higher than the negative control.5Saturation Binding Assay0.415 + 0.047 μMDetectionSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0357 251855032014Selection of peptidoglycan-specific aptamers for bacterial cells identificationAntibac2TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC555'-TCGCGCGAGTCGTCTG-N24-CCGCATCGTCCTCCC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923) and Escherichia Coli (E. Coli) TOP10PeptidoglycanIdentify aptamers that bind to the peptidoglycan of bacterial cells.High binding affinity for S. aureus, as the radioactivity of Antibac2 was 22 times higher than the negative control.5Saturation Binding Assay1.261 + 0.280 μMDetectionSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0358 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-27ACGGCTCGCACTCTCTGATTT-GGCATAGCTGCCGGGAGGGGGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.The binding signal of EcA5-27 for E. coli NSM59 was notably higher (~ 1.5-fold) than for laboratory strains of E. coli, and 8.5- and 56-fold to additional uropathogenic isolates compared with laboratory strains.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)110 nMDetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/ABest CandidateN/AN/A
ABdb_0359 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-01ACGGCTCGCACTCTCTGATTT-CGGCACCCCGTCGCTATGTTGACC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0360 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-02ACGGCTCGCACTCTCTGATTT-GGGGAGGTGGCGACCGCTTCTCAG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0361 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-03ACGGCTCGCACTCTCTGATTT-GGGGTCAGATATTAAACCGTGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0362 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-04ACGGCTCGCACTCTCTGATTT-GGGAGAGGGAGTGGTCTGGGAGAG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0363 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-05ACGGCTCGCACTCTCTGATTT-GCGGGCTTCGACACAGTGGGGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0364 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-06ACGGCTCGCACTCTCTGATTT-GGGAGGGGCGGCGAAGGAGTGGCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0365 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-07ACGGCTCGCACTCTCTGATTT-GGAAGCGGTGGGGATCGTGTGTGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0366 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-08ACGGCTCGCACTCTCTGATTT-GGGAGCAAATCCGGAATGTGGGGC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0367 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-09ACGGCTCGCACTCTCTGATTT-GGCGGAGGGGTTCGGGGTTGGCGC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0368 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-10ACGGCTCGCACTCTCTGATTT-GGCGGGGCGTGGGGGATGTGTGTG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0369 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-11ACGGCTCGCACTCTCTGATTT-GGAATGCGAAGTGTGGCCTAGGGC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0370 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-12ACGGCTCGCACTCTCTGATTT-GGGCGGGGGGGGATTCCGAGGCGC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0371 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-13ACGGCTCGCACTCTCTGATTT-GGGGGGGTGGCATTTTGGGGTGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0372 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-14ACGGCTCGCACTCTCTGATTT-GCGGGGAAAGAGAAGGAAGCGTCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0373 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-15ACGGCTCGCACTCTCTGATTT-GGGCCCGAGTGGCGGTAGTTTCAG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0374 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-16ACGGCTCGCACTCTCTGATTT-GGGGGGTGGTGAAGGCCTGGGGGA-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0375 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-17ACGGCTCGCACTCTCTGATTT-GGGCCGGAGGGGCGCCTGCACCCA-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0376 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-18ACGGCTCGCACTCTCTGATTT-GGGGTTAGGCGAGGGGGGTGGGTG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0377 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-19ACGGCTCGCACTCTCTGATTT-CGGACGGTAGGGAAGGGGGGGGCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0378 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-20ACGGCTCGCACTCTCTGATTT-GGCGAGGCAGGGTGCGGGGGCCCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0379 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-21ACGGCTCGCACTCTCTGATTT-GCGTGTGGTGGGTGAGGGGTCTGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0380 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-22ACGGCTCGCACTCTCTGATTT-GGGGATAGCAGGACAATGAGGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0381 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-23ACGGCTCGCACTCTCTGATTT-GGCCGCGTGTGTGTCCGACTGGTG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0382 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-24ACGGCTCGCACTCTCTGATTT-GGGCGAGGAGAGAGGCGGAGGGCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0383 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-25ACGGCTCGCACTCTCTGATTT-GCCGTGGTGTGTGTGATGGTCGGT-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0384 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-26ACGGCTCGCACTCTCTGATTT-GGCTTGGCTCCTCACGGGGGGTGA-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0385 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-28ACGGCTCGCACTCTCTGATTT-GGAGGGGTTGACCATGACCGGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0386 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-29ACGGCTCGCACTCTCTGATTT-GGGGGAAGGGCCAATGGATTGTGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0387 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-14GCTGCAATACTCATGGACAG-GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC-GTCTGGAGTACGACCCTGAA805'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.With a PI cutoff value of 40%, the ic-ELAA had higher positive detection rates than two commercial indirect ELISA.11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)15.61 ± 2.36 nMDetectionSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0388 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-3GCTGCAATACTCATGGACAG-GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG-GTCTGGAGTACGACCCTGAA805'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.N/A11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)32.81 ± 7.75 nMDetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0389 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-51GCTGCAATACTCATGGACAG-GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC-GTCTGGAGTACGACCCTGAA785'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.N/A11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)20.81 ± 4.48 nMDetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0390 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-49GCTGCAATACTCATGGACAG-GCAGACGTCAGAACAATACCAATACTAATCCTTGGACCGC-GTCTGGAGTACGACCCTGAA805'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.N/A11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)N/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0391 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-20GCTGCAATACTCATGGACAG-CAACGAACAATACATTATCTACATCACATTGCCCCGCGTG-GTCTGGAGTACGACCCTGAA805'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.N/A11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)N/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0392 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-48GCTGCAATACTCATGGACAG-GCACGACATAACAACACTTATAGACTACTTCCCCCCTCGC-GTCTGGAGTACGACCCTGAA805'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.N/A11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)N/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0393 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-37GCTGCAATACTCATGGACAG-GTCCAAACACTATCTCTTCACTTGCTGTTGTTCCCGTGGC-GTCTGGAGTACGACCCTGAA805'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.N/A11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)N/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0394 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-82GCTGCAATACTCATGGACAG-GCACAACACATTAGACTTTGCTCTTCGGTCCCTCCGGCT-GTCTGGAGTACGACCCTGAA795'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.N/A11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)N/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0395 244176932014Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serumWKB-53GCTGCAATACTCATGGACAG-GCTACCACCAAACATACGCACTCGTCGTCTGCCCTATGTG-GTCTGGAGTACGACCCTGAA805'-GCTGCAATACTCATGGACAG-N40-GTCTGGAGTACGACCCTGAA-3'ssDNAMycoplasma Bovis (M. Bovis)P48 proteinIndirect competitive enzyme-linked aptamer assay (ic-ELAA) for the serological detection of M. bovis.N/A11Indirect Enzyme-Linked Aptamer Assay (i-ELAA)N/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0405 248139972014Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEXG43UAAUACGACUCACUAUAGGGAGACAAGACUAGACGCUCAA-AAUAGGAACUUAGAACAACCCUCUCCCUCAUGUAGCGAACCCGAACCAGG-UUCGACAUGAGACUCACAACAGUUCCCUUUAGUGAGGGUUAAUU134N/AssRNAMycobacterium Tuberculosis (H37Rv)EsxG proteinIdentify aptamers against EsxG over its homologue EsxA.The affinity of aptamer G43 to EsxG was about ten times higher than the affinity of aptamer G78 and also showed no significant binding to EsxA.5Surface Plasmon Resonance (SPR)8.04 ± 1.9 nMDetectionSPR based SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0406 248139972014Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEXG78UAAUACGACUCACUAUAGGGAGACAAGACUAGACGCUCAA-GCGUUUAAACGUUGCACCGUCGUUGACGGGACCGCUUGAGACAUUCGCUC-UUCGACAUGAGACUCACAACAGUUCCCUUUAGUGAGGGUUAAUU134N/AssRNAMycobacterium Tuberculosis (H37Rv)EsxG proteinIdentify aptamers against EsxG over its homologue EsxA.G78 showed some binding to EsxA, though a significantly lower binding signal was observed (p < 0.01) when compared to that of EsxG.5Surface Plasmon Resonance (SPR)78.85 ± 9.4 nMDetectionSPR based SELEXN/AN/AN/AN/AN/AN/A
ABdb_0407 248139972014Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEXG31UAAUACGACUCACUAUAGGGAGACAAGACUAGACGCUCAA-UAACGACUCACCAACACAUCGACUAAUUUCCCUAAAUGACUUAACUGAAU-UUCGACAUGAGACUCACAACAGUUCCCUUUAGUGAGGGUUAAUU134N/AssRNAMycobacterium Tuberculosis (H37Rv)EsxG proteinIdentify aptamers against EsxG over its homologue EsxA.N/A5Surface Plasmon Resonance (SPR)N/ADetectionSPR based SELEXN/AN/AN/AN/AN/AN/A
ABdb_0408 248139972014Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEXG70UAAUACGACUCACUAUAGGGAGACAAGACUAGACGCUCAA-ACUCGUGACACUUACGAACGACCUAUGGCGGGAGAAUCCUAGCCGCUACG-UUCGACAUGAGACUCACAACAGUUCCCUUUAGUGAGGGUUAAUU134N/AssRNAMycobacterium Tuberculosis (H37Rv)EsxG proteinIdentify aptamers against EsxG over its homologue EsxA.N/A5Surface Plasmon Resonance (SPR)N/ADetectionSPR based SELEXN/AN/AN/AN/AN/AN/A
ABdb_0409 247638182014Rapid fluorescent detection of Escherichia coli K88 based on DNA aptamer library as direct and specific reporter combined with immuno-magnetic separationApt B12TGGGAGCTCAGAATAAACGCTCAA-GGCACACAGGACTATACAGTGTTGCAGTGTTGCTG-TTCGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)Whole cellDNA aptamer library for rapid detection of ETEC K88 combined with immuno-magnetic separation (IMS).Exhibit detection limit of 1.1 × 10(3) CFU/ml in pure culture and 2.2 × 10(3) CFU/g in artificially contaminated faecal sample with aptamer library.13Fluorescence Binding Assay15 ± 4 nMDiagnosticWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0410 247638182014Rapid fluorescent detection of Escherichia coli K88 based on DNA aptamer library as direct and specific reporter combined with immuno-magnetic separationApt H11TGGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)Whole cellDNA aptamer library for rapid detection of ETEC K88 combined with immuno-magnetic separation (IMS).Exhibit detection limit of 1.1 × 10(3) CFU/ml in pure culture and 2.2 × 10(3) CFU/g in artificially contaminated faecal sample with aptamer library.13Fluorescence Binding Assay66 ± 7 nMDiagnosticWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0411 247638182014Rapid fluorescent detection of Escherichia coli K88 based on DNA aptamer library as direct and specific reporter combined with immuno-magnetic separationApt C09TGGGAGCTCAGAATAAACGCTCAA-TGGCCGTGTGGATAGAGGCGTGTTGTATGGGTGTG-TTCGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)Whole cellDNA aptamer library for rapid detection of ETEC K88 combined with immuno-magnetic separation (IMS).Exhibit detection limit of 1.1 × 10(3) CFU/ml in pure culture and 2.2 × 10(3) CFU/g in artificially contaminated faecal sample with aptamer library.13Fluorescence Binding Assay52 ± 8 nMDiagnosticWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0412 247638182014Rapid fluorescent detection of Escherichia coli K88 based on DNA aptamer library as direct and specific reporter combined with immuno-magnetic separationApt H12TGGGAGCTCAGAATAAACGCTCAA-GGGGAGGCAGTGTGTTGTGCCGTGTGTATGCTTGG-TTCGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)Whole cellDNA aptamer library for rapid detection of ETEC K88 combined with immuno-magnetic separation (IMS).Exhibit detection limit of 1.1 × 10(3) CFU/ml in pure culture and 2.2 × 10(3) CFU/g in artificially contaminated faecal sample with aptamer library.13Fluorescence Binding Assay106 ± 12 nMDiagnosticWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0417 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT17CGCGTCAGAGGTGTGTCGGGGCTGTGTAGATCTACATGGG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0418 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT12CAGTCGCTTTCCGTTCTCCGGCAGGTTCATTGTGGTTTCG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0419 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT11CATATCAGGTCGTCACCGTAACAGAGCTCTCGCAATCACG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0420 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT9CAAAGGCAGCAGTAGCATGGCGATTTACATCAATTATTGG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0421 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT8CAATATCAGAAGTAGCGCGAAGGACGACATGTCAGGAAGG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0422 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT7CACACATCCTCGCAGCCTCGTACCTGATTCCAGTCTATTG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0423 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT6CCAGGAAGGCGAGAGCCGAGAAGCGATCCTTGGGTATAGG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0424 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT5CGGCATCCGTTCACGTACCTGTCCTAGTTATCACCGTTTG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0425 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT5.1CAAGCAGAGTTCCGAGACACAGTACCACACGCATATCCGG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0426 255361052014A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matricesFT4CACACAGAGACGGTGAAGGCGCCGACCAGTTCCTAAAGAG40WAP40mssDNAFrancisella TularensisWhole cellDeveloped a two-step enrichment process using DNA aptamer cocktail for improved cultivation and detection of F. tularensis in lettuce and soil.This two-step approach resulted in a lower limit of detection from a starting inoculum of 1 cell/mL.11N/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0435 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-2AGTATACGTATTACCTGCAGC-TGGGGGGTGGTTGGGGGTAGTATATCGGGTCAGTGGTGCG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0436 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-103AGTATACGTATTACCTGCAGC-GGGAGGCGTGAAGTGTGATGGTGTGGGGGGTAGTGGCCGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0437 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-109AGTATACGTATTACCTGCAGC-CCGCTCAACAAGGTGCTACAAAGATCCTGTGTCCCTTGGC-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0438 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-116AGTATACGTATTACCTGCAGC-TACTCGTTATTTCGTAGCACTTTTCCCCACCACCTTGGTG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.LM6-116 showed strong genus-specific binding affinity when screened against five different species within the Listeria genus.6Flow Cytometry74.4 ±52.69 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0439 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-118AGTATACGTATTACCTGCAGC-CTGGTATAGACGTTATTCGTCCGCTCTTGTATCCTTGTGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0440 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-121AGTATACGTATTACCTGCAGC-TGTCGGTGGGGGGTGGCTTGAGTACGTGACGTGGTGTGGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0441 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM12-2AGTATACGTATTACCTGCAGC-CCATTGTTCTATCGCTAGCTCGCTCTGGCCAGCTCCTTGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0442 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM12-6AGTATACGTATTACCTGCAGC-TCTGTGTTCCGTTTTCGATTCTTACTGTGTTTTCGGGTGC-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow Cytometry106.4 ±43.91 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0443 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM12-13AGTATACGTATTACCTGCAGC-TGGGGGGGGGGGGAGGGGTCGGTGAGCGTGGGAGGAGGAG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0444 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM12-74AGTATACGTATTACCTGCAGC-CCGACCTTCTATCCTTCCAGTTTTTTGTAAACTCTTCTGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0446 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmA_Apt1CACAGCGGGACAGUUUAGC-AUUAGCUAUCUUCCACGAUUCGAUCAGGCAGUACGUCGU-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer mA_Apt1 (~40% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/ABest CandidateN/AN/A
ABdb_0447 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmB_Apt23CACAGCGGGACAGUUUAGC-ACAGUUAUCCGACUGCUAUUCGAUCAGGCAGUACGUAGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells. A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer mB_Apt23 (~20% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0448 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmC_Apt41CACAGCGGGACAGUUUAGC-CAGGCUGUUCUGACGCAUAAGGAAUGCGCUGUUGCAGAG-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.mC_Apt41 neutralized 0.007 pmole of Stx2 (≤60% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0449 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmD_Apt46CACAGCGGGACAGUUUAGC-UUGGUCCUGCUUUGGAUAGUCGCGAAAGGGGUGCCACUG-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0450 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspA_Apt1CACAGCGGGACAGUUUAGC-ACAGUUAUCCGACUGCUAUUCGAUCAGGCAGUACGUAGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0451 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspB_Apt14CACAGCGGGACAGUUUAGC-ACCGAGCGGUUUUACGUCUCAAGUAGUAUCCCGUUUUGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Did not inhibit Stx2 binding.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0452 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspC_Apt22CACAGCGGGACAGUUUAGC-AUUAGCUAUCUUCCACGAUUCGAUCAGGCAGUACGUCGU-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer pC_Apt22 (≤60% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0453 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspD_Apt25CACAGCGGGACAGUUUAGC-UUGCCAUCCUGUACUAUGCUCUAUCGGGCGGUUUAGUGAUCCUUCGUCCAACUAUC-GGUAGGUGGGUGCGUCUAAA955'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Did not inhibit Stx2 binding.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0463 250963912014Development of ssDNA aptamers for the sensitive detection of Salmonella typhimurium and Salmonella enteritidisSe1CACACCGGAAGGGATGCCACCTAAACCCC295'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellIdentify aptamers that can be used as detection probes in a polyvalent directed aptamer polymer (PDAP), which specifically bind to S. enteritidis and S. typhimurium.The binding efficiency of the PDAP of Se1 was approximately 100-fold higher than that of the single aptamer.10Fluorescence Spectroscopy4.66 ± 0.35 μM (of single aptamer) and 57.86 ± 14.5 nM (of PDAP)DetectionWhole Cell-SELEX5'-FAM Labeled, 3'-Thiolated and Poly-D-Lysine (PDL)N/AN/AN/AN/AN/A
ABdb_0464 250963912014Development of ssDNA aptamers for the sensitive detection of Salmonella typhimurium and Salmonella enteritidisSe2CACAGATGACGTCTGGCACATAATTAACAC305'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellIdentify aptamers that can be used as detection probes in a polyvalent directed aptamer polymer (PDAP), which specifically bind to S. enteritidis and S. typhimurium.The binding efficiency of the PDAP for Se2 was approximately 80-fold higher than that of the single aptamer.10Fluorescence Spectroscopy3.83 ± 0.10 μM (of single aptamer) and 55.84 ± 24.7 nM (of PDAP)DetectionWhole Cell-SELEX5'-FAM Labeled, 3'-Thiolated and Poly-D-Lysine (PDL)N/AN/AN/AN/AN/A
ABdb_0465 250963912014Development of ssDNA aptamers for the sensitive detection of Salmonella typhimurium and Salmonella enteritidisSt1CCGATGTCCGTTAGGGCTCCTCCATAGAT295'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that can be used as detection probes in a polyvalent directed aptamer polymer (PDAP), which specifically bind to S. enteritidis and S. typhimurium.The binding efficiency of the PDAPs of St1 was approximately 20-fold higher than that of the single aptamer.10Fluorescence Spectroscopy0.530 ± 0.01 μM (of single aptamer) and 25 ± 5.6 nM (of PDAP)DetectionWhole Cell-SELEX5'-FAM Labeled, 3'-Thiolated and Poly-D-Lysine (PDL)N/AN/AN/AN/AN/A
ABdb_0468 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesb03TGGGAGCTCAGAATAAACGCTCAA-TTTTCCTGTTGTTGTTGTTTTTGGGGTTTTTTGTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0469 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesh03TGGGAGCTCAGAATAAACGCTCAA-ATTTGTTTTGTTGGTGTTGTTGGCAGGTTTTGTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5′-DIG (Digoxigenin) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0470 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesh04TGGGAGCTCAGAATAAACGCTCAA-TTTTTTGTTTTAGTTTTGTTTTGGTTTGTAGTGTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0471 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesc04TGGGAGCTCAGAATAAACGCTCAA-TGTTTTTTTGTGGGTTTGTTTTTTTCTTCTTTTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0472 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesc01TGGGAGCTCAGAATAAACGCTCAA-TGTCGTTGTCGTTTTGTGTTTTTTTGGTGTTTTTGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0473 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesa04TGGGAGCTCAGAATAAACGCTCAA-TTGTTTGTTTTGGTTTTTTCGTTTTTTTTTGTTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0474 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesb02TGGGAGCTCAGAATAAACGCTCAA-TATGTTGTCTTTTGTTTTGTGTTTTTTGTTGGTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0475 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesf01TGGGAGCTCAGAATAAACGCTCAA-GTGGTATAGTTCTTTTTTTTTGTTTTTTTGTTTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)150.8 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0476 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesb04TGGGAGCTCAGAATAAACGCTCAA-TGGTCTTGTCGTTTTATTGTTTTTTTTTTTCTGTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0477 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenese01TGGGAGCTCAGAATAAACGCTCAA-CTTTGTTCTTCTTTGCTTTTTTTTTCTTTTTTTGTT-CGACATGAGGCCCGGATCA795'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.Digoxigenin-aptamer could bind to the target L. monocytogenes with much higher specificity, resulting in a clear fluorescent signal.9Aptamer-Linked Immobilized Sorbent Assay (ALISA)60.01 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0478 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesh02TGGGAGCTCAGAATAAACGCTCAA-TGTTTGTCTGTTTTCGTTTGTCTATTTGGTTCAGTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0479 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenese05TGGGAGCTCAGAATAAACGCTCAA-GCTGTTTTTTTCGTGTTGGGTTTTTTGTTTTGTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0480 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesd02TGGGAGCTCAGAATAAACGCTCAA-TTGTTTTTTTTTTTTGGTGTTTTTTTTTTATTTT-CGACATGAGGCCCGGATCA775'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0481 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesc03TGGGAGCTCAGAATAAACGCTCAA-TTGTTTGTTTTTTTTTTGGCTGTTATTTTGTTTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0482 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesd03TGGGAGCTCAGAATAAACGCTCAA-CTGTCTTTTGTTTTTGTTGTGTTTTTTTGTTTTTGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0483 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesg02TGGGAGCTCAGAATAAACGCTCAA-TGTCACTAGGCTTTTGGGATTCTCTGTGCATTTTGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0484 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesg01TGGGAGCTCAGAATAAACGCTCAA-TGTTGGTCTGTGTTATATTTTTTGTATCTCGTTCTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0485 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesg03TGGGAGCTCAGAATAAACGCTCAA-TTCCTGGTTTATGTTGGGTTTTTTTGTTTCCTGGTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0486 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesf04TGGGAGCTCAGAATAAACGCTCAA-GCCTTCTGCCTGTGTACGTTAGTGTTTGTGTCTATTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0487 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesd04TGGGAGCTCAGAATAAACGCTCAA-GGCTTTTTCGTCTTGTTCCTTGTTATTTGTTGTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0488 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenese03TGGGAGCTCAGAATAAACGCTCAA-GGTTGTGCTTTGTATTCTCTTTTCTGTTTGATTTTTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0489 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesh01TGGGAGCTCAGAATAAACGCTCAA-TGTCTTTCCGTCGATATGCTGCTGACGCTTGGGGCTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0490 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesa02TGGGAGCTCAGAATAAACGCTCAA-TCACTGTTTGTCTGTTTGCTGGGTTTTTTGTTTTGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0491 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesc02TGGGAGCTCAGAATAAACGCTCAA-CTCTCAATGTGTATCTTGTTGCCGTTGTTCTTGTGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0492 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesd05TGGGAGCTCAGAATAAACGCTCAA-TGTAGTTTTTGTTTTGCGTGGTTTTAGATGCTGGCTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0493 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenese02TGGGAGCTCAGAATAAACGCTCAA-TGTACCGGTGGTATTGTTTATGGTTGGCTGTTCTGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0494 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesc05TGGGAGCTCAGAATAAACGCTCAA-CAGCCGGGGTGCGGGGCAAGGAGAGCGCGGTCAATTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0495 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesf02TGGGAGCTCAGAATAAACGCTCAA-CGGGGTGGGACTTTTAGCGTATTTGTGCTGGCGTGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0496 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenese04TGGGAGCTCAGAATAAACGCTCAA-GGGGGCGAGTGGAAGAGGCAGGGAAGGGAAATGCGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0497 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesg04TGGGAGCTCAGAATAAACGCTCAA-GGGGGGGGGGTATTGGCAGAGGTGGTTAGGTGGCCCTT-CGACATGAGGCCCGGATCA815'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0498 https://doi.org/10.1016/S2095-3119(14)60766-82014In vitro Selection of DNA Aptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenesa03TGGGAGCTCAGAATAAACGCTCAA-GGCGGGAGGAGACCGACCAGGCTCAGGGTGGGGCGTT-CGACATGAGGCCCGGATCA805'-GGGAGCTCAGAATAAACGCTCAA-35N-CGACATGAGGCCCGGATC-3'ssDNAListeria MonocytogenesWhole cellIdentify aptamers and developed a fluorescently-labeled aptamer assay for detecting L. monocytogenes.N/A9Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0502 260253072015Efficient suppression of biofilm formation by a nucleic acid aptamerAptamer 3GGGAGCTCAGAATAAACGCTCAA-GGCAGGACAACAGCGTGTAGTATCAGCTTACGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNASalmonella CholeraesuisFlagellaIdentify an aptamer that binds to flagella and inhibits biofilm formation by restricting its rotational frequency and covering its binding sites that attach to the matrix surfaces.13.8% survival ratio of living cells in biofilms with 1.1 μM aptamer and 5 μg/mL ampicillin sodium.14Fluorescence Spectroscopy41 ± 2 nMTherapeuticsSELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0503 260253072015Efficient suppression of biofilm formation by a nucleic acid aptamerAptamer 1GGGAGCTCAGAATAAACGCTCAA-GGCCAGCAGCGAGTGTGGAGTATTGTGTTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNASalmonella CholeraesuisFlagellaIdentify an aptamer that binds to flagella and inhibits biofilm formation by restricting its rotational frequency and covering its binding sites that attach to the matrix surfaces.N/A14Fluorescence Spectroscopy50 ± 2 nMTherapeuticsSELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0504 260253072015Efficient suppression of biofilm formation by a nucleic acid aptamerAptamer 2GGGAGCTCAGAATAAACGCTCAA-GGGCAGGTGTTATGTGTACTGCTACAGTGTGGTTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNASalmonella CholeraesuisFlagellaIdentify an aptamer that binds to flagella and inhibits biofilm formation by restricting its rotational frequency and covering its binding sites that attach to the matrix surfaces.N/A14Fluorescence Spectroscopy54 ± 2 nMTherapeuticsSELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0505 260253072015Efficient suppression of biofilm formation by a nucleic acid aptamerAptamer 4GGGAGCTCAGAATAAACGCTCAA-GGCACGGCATGTGTGGTATGTGGTGCCTGTACTCG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNASalmonella CholeraesuisFlagellaIdentify an aptamer that binds to flagella and inhibits biofilm formation by restricting its rotational frequency and covering its binding sites that attach to the matrix surfaces.N/A14Fluorescence Spectroscopy53 ± 5 nMTherapeuticsSELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0506 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 36CCGTCAGCCGAGGACCACAC-TTGGTTGCTGAATCCCCTCGTCTTGGCTTTCTTTGTCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.Mtb36 aptamer bound to M. tuberculosis H37Ra bacteria more than 4 times for M. bovis and 70 times for E. coli bacteria.7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)5.09 ±1.43 nMDetectionWhole Cell-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0507 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 17CCGTCAGCCGAGGACCACAC-GCGTCAATATGCTCGAGTCCCTTACTCCGTAATCTTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0508 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 57CCGTCAGCCGAGGACCACAC-GAGGCGGGGCGTACATGATGGAGGTGTTGTGTTCTTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0509 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 25CCGTCAGCCGAGGACCACAC-ATAGGAAGTATGCGGTCGTAGTTAATCGCCTGTCCTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0510 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 32CCGTCAGCCGAGGACCACAC-CTGGGCTCACTCACTGGCGTATCATTCGTCCGCGGTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0511 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 3CCGTCAGCCGAGGACCACAC-CGCTTCACGTGTCAGTGAATTTTCTCCATCGTTTGGTGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0512 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 40CCGTCAGCCGAGGACCACAC-TGTAGCGCATCTACGGGCTCTTCATTACGTCTATATCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0513 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 33CCGTCAGCCGAGGACCACAC-TTCACACACGGGTATAAACGCTCTGGTATGTCAAGCCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0514 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 35CCGTCAGCCGAGGACCACAC-TCGGACCTAGTGCTAGGGTCTCTAAGAAGTATCGGGGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0515 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 49CCGTCAGCCGAGGACCACAC-TTCTCCGATCTTAGTCAACTTGGACCATGAATGCGGGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0516 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 46CCGTCAGCCGAGGACCACAC-TATTTACGCTGACCGACGATTTTCTATCAGAGTGCCGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0517 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 13CCGTCAGCCGAGGACCACAC-CGATCACTGTACAGTCCTGGATAAGCCGTTCTTTCCGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0518 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 11CCGTCAGCCGAGGACCACAC-CGCCGGCCTTCTTACAAGACCTGTTCAATTCCCAGTGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0519 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 50CCGTCAGCCGAGGACCACAC-AGGCTCACGCCCTGTCAATACTGCCTCTTGTCCTTCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0520 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 38CCGTCAGCCGAGGACCACAC-ATTCGGCTGAAGACCCGAGCCGGTCATCCGGTGTTCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0521 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 28CCGTCAGCCGAGGACCACAC-CTCCATCACGTGTAGTCAGCTGACCATTGATCGTGCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0522 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 8CCGTCAGCCGAGGACCACAC-CTATCCCGTGGTCCATTGCTTTTCCCGGTCTCTTCTCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0523 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 52CCGTCAGCCGAGGACCACAC-CTTTGTGCCGGGAAAAGACTGGCCTGTGTTGACTTGCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0524 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 2CCGTCAGCCGAGGACCACAC-CGGCGCTTTTCTCATCCTACCTCCGCTCTTACCTGTATGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0525 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 9CCGTCAGCCGAGGACCACAC-GCCGTGCTGAGTCTGTCAGCAGTTTGCTAGTCTTCCCTGC-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0526 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 44CCGTCAGCCGAGGACCACAC-TTACACGAAGGCTCGGCCCATCTCTCTCGTGTCACTTCGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0527 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt1ATGCGGATCCCGCGC-CGAGTGAGGGCGAGGCGCGCTCCTGCCGGT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.IC₅₀ of 28.94 ± 0.002 nM and showed a MIC of 5.36 μg/ml against MDR (M22, M23, and P887) and XDR (X24, X59) strains.10Enzyme-Linked Immunosorbent Assay (ELISA)1.06 ± 0.10 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0528 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt6ATGCGGATCCCGCGC-CGGCCAGGGGACGAGCGCGCCCTGATCGTG-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.IC₅₀of 22.35 ± 0.001 nM and showed MIC of 6.24 μg/ml against MDR (M22, M23, and P887) and XDR (X24, X59) strains.10Enzyme-Linked Immunosorbent Assay (ELISA)0.210 ± 0.05 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0529 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseApt1_M3 (17-mer)ATGCGGATCCCGCGC-GAGGGCGAGGCGCGCTC-GCGCAAGCTTCGCGC475'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.Significant growth inhibition against MDR-TB and XDR-TB strains of tuberculosis with a very low MIC of 5.36 μg/ml.10Enzyme-Linked Immunosorbent Assay (ELISA)4.90 μMTherapeuticsDNA-SELEX5'-BiotinylatedMtb-Apt1 (17-mer) and Mtb-Apt6 (20-mer) do not exhibit significant toxicity at the tested concentrations ranging from 0 to 20 μM.N/AN/AN/AN/A
ABdb_0530 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseApt6_M3 (20-mer)ATGCGGATCCCGCGC-CAGGGGACGAGCGCGCCCTG-GCGCAAGCTTCGCGC505'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.Significant growth inhibition against MDR-TB and XDR-TB strains of tuberculosis with a very low MIC of 6.24 μg/ml.10Enzyme-Linked Immunosorbent Assay (ELISA)364 nMTherapeuticsDNA-SELEX5'-BiotinylatedMtb-Apt1 (17-mer) and Mtb-Apt6 (20-mer) do not exhibit significant toxicity at the tested concentrations ranging from 0 to 20 μM.N/ABest CandidateN/AN/A
ABdb_0531 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt2ATGCGGATCCCGCGC-GGCACCCAGTGTGGCGCGCCTCCCTCCGTC-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)0.286 ± 0.64 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0532 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt3ATGCGGATCCCGCGC-GCCCACCTGTGGGGCGCGCCTCCCTCCGTC-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.Showed moderate to lower inhibition specificities.10Enzyme-Linked Immunosorbent Assay (ELISA)0.677 ± 0.14 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0533 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt4ATGCGGATCCCGCGC-GCCCACGTGTGGTGCGCGCCTCCTCGTAGT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.Showed moderate to lower inhibition specificities.10Enzyme-Linked Immunosorbent Assay (ELISA)0.956 ± 0.20 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0534 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt5ATGCGGATCCCGCGC-GGCACCCAGTGTGGCGCGCCTCCTCGTAGT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)2.03 ± 0.12 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0535 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt7ATGCGGATCCCGCGC-ACGCGACAGCAGTGCGCGCCCCGTCCCGGT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)1.02 ± 0.13 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0536 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt8ATGCGGATCCCGCGC-CGACGGAGGGAGGCGCGCCACACTGGGTGC-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)0.55 ± 0.05 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0537 259882432015Development of ssDNA aptamers as potent inhibitors of Mycobacterium tuberculosis acetohydroxyacid synthaseMtb-Apt9ATGCGGATCCCGCGC-GCACCGGCAGGAGCGCGCCTCGCCCTCACT-GCGCAAGCTTCGCGC605'-ATGCGGATCCCGCGC-N30-GCGCAAGCTTCGCGC-3'ssDNAMycobacterium Tuberculosis MDR (M22, M23, and P887) strains and XDR (X24, X59) strainsAcetohydroxyacid synthase (AHAS)Identify aptamers that bind to and inhibit the activity of the AHAS enzyme and bacterial growth.N/A10Enzyme-Linked Immunosorbent Assay (ELISA)1.66 ± 0.22 μMTherapeuticsDNA-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0538 256243252015Neutralization of staphylococcal enterotoxin B by an aptamer antagonistA11ATACCAGCTTATTCAATT-TAGGCGGTGGAGATAGTAAGTGCAATCTAGGCGGTGGAGATAGTAAGTGCAATCTATGCC-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and block SEB-mediated toxic shock (TSS) in vitro.Inhibited SEB-mediated PBMC proliferation of 93% and TNF-α, IL-1β, IL-6, IL-2, and IFN-γ in culture supernatants were reduced by 77, 97, 64, 96, and 99%, respectively. 90% survival of mouse models of SEB-induced TSS.11Fluorescence Spectroscopy64 nMTherapeutics/ImmunmodulatorySELEX5′-Polyethylene glycol (PEG) with 5'-Amidation (NH₂) and 3’-Inverted Thymidine (3'-idT) and 5'-Biotinylated or 5'-FITC LabeledShowed no cytotoxic effects on PBMCs.N/AN/AN/AN/A
ABdb_0539 256243252015Neutralization of staphylococcal enterotoxin B by an aptamer antagonistA2ATACCAGCTTATTCAATT-GATCGATCGATCAGTCAGTCGACCAACGCACGACACGCATCGCTCTATCTCGTCGAACAG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and block SEB-mediated toxic shock (TSS) in vitro.A2 achieved 34% PBMC proliferation inhibition.11Fluorescence Spectroscopy26 nMTherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0541 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1C10AGCAGCACAGAGGTCAGATG-TATACTTCTAAAATTTGTTTGTATCTACGATGTTCTTCGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.Have a limit of detection of 6 ng/mL with a range of 0.01–10 μg/mL of SEC1.12Fluorescence Spectroscopy65.14 ± 11.64 nMBiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0542 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C2C36.2AGCAGCACAGAGGTCAGATG-TATCAGAATTAATGCTCTCGTAATTTTCGAATCGTCGTGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence Spectroscopy49.43 ± 11.76 nMBiosensorSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0543 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C3C34.1AGCAGCACAGAGGTCAGATG-CTTTCATTTTTATTCTTTTTGACTGTATTTTATGTACCAT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0544 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C4C4AGCAGCACAGAGGTCAGATG-CTAACCTAATTCGACCGTGTATTCTTCTGCGTTATTTACC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0545 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C5C10AGCAGCACAGAGGTCAGATG-TATACTTCTAAAATTTGTTTGTATCTACGATGTTCTTCGT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0546 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C6C9AGCAGCACAGAGGTCAGATG-TCCATTATCAGGTTCTTTATTCTGTTGTTCAACTTATTAA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence Spectroscopy154.9 ± 45.67 nMBiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0547 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C7C32.1AGCAGCACAGAGGTCAGATG-TTTTAGATTTAGCTCTTATTTGTTCGAGCAATCCCAAAGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0548 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C8C2AGCAGCACAGAGGTCAGATG-GCCAACTCAATTTTTTGTTTTGTCTTTCCGATTGATCTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0549 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C9C6AGCAGCACAGAGGTCAGATG-CATATCGAATTTTCCTCGTGATTTTTCTTTCATTCTTAGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0550 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C10C11AGCAGCACAGAGGTCAGATG-TAGTTAATGGATTTTTCTTCTTTATCTTCTTATTTCTTGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0551 250531022015Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C11C30AGCAGCACAGAGGTCAGATG-ATGTTTTCCCTTATCACTACTTTTATTTGTCTTTTTGCAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin C1 (SEC1)Identify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect SEC1 in milk samples.N/A12Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0552 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12UGUAAUUCUGCCAUUCUUUUUGGGGCGGAAUACAGGAUGU405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.fmA12-functionalized AgNPs resulted in specific S. aureus antimicrobial activity with only a 1.6 ± 1.0% survival of 12598 cells and 51.9 ± 2.0% survival of 10832 cells.8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)95 nM (by SPR) and 67.1 ± 6.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/A76% fmA12 survives in 10% mouse serum at 37°C over 24 h, and in 50% serum, 85.0% fmA12 survives after 5 h.N/A~52 h for fmA12.N/A
ABdb_0553 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmF07AUACGCUGCUCACUAAAGUCGGGAGUACUCCAAUGUUUUC405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)38.9 ± 4.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/ABest CandidateN/AN/A
ABdb_0554 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmE09AUCUAGCAAGCACUUGCAAACAUUCGAUAACGGGGAUUC395'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)418 ± 112 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0555 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmG12UGCCAAUGUAACUCAACACUCUUCAUUGAGCUGUCGGGCA405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)475 nM (by SPR) and 220 ± 24 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0556 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ3N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.7 ± 3.5 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0557 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ6N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)63.1 ± 7.3 μMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0558 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ9N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.1 ± 16.7 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0559 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-CA 20CACACACGGAACCCCGACAACATACATACGGTGAGGGTGG40N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 55% increase in the gated fluorescence intensities above background.8Flow Cytometry7 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0560 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-CA 20PTTCACGGTAGCACGCATAGG-CACACACGGAACCCCGACAACATACATACGGTGAGGGTGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 61% increase in the gated fluorescence intensities above background.8Flow Cytometry12 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0561 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 9GGGGAGGAGAAAAAGAGGACCAGAGTAACGATTCGTGGGG40N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 55% increase in the gated fluorescence intensities above background.8Flow Cytometry71 ± 23 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0562 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 9PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAAAAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.D-Cells 9P showed an increase in the gated fluorescence above background by 65%.8Flow Cytometry44 ± 8 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0563 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-Cells 1PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAAAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT79N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow Cytometry20 ± 3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0564 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-Cells 1GGGGAGGAGAAAAGAGGACCAGAGTAACGATTCGTGGGG39N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0565 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 20PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAATAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0566 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGGCGTGGGAGGCAATGCCTTGCTTGTAGGCTTCCCCTGTGCGCG-GGTGGATCCATATTCCTACTCG1075'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0567 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA14CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT-GGTGGATCCATATTCCTACTCG1085'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence Spectroscopy3.49 ± 1.43 nMDetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0568 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA15CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACGCGCAAACAGATTAACACTCCGCCTAAGTCTGCCGCACGC-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0569 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA20CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCAGCGGGGGCTGCGCGGTGGAGTGCTGTGGGCG-GGTGGATCCATATTCCTACTCG985'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0570 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGAGCCAGGATGGGAGGTCTGTAGGTCTGCGGGGCGTG-GGTGGATCCATATTCCTACTCG1045'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0571 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB6CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGTCGGTGTCTGCCGGGGGATGTGGAGGCTGGGTGTTGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0572 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB7CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGGGCGGGCTACCTGGCTAGTACGCCATGATGCCTGCACGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0573 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB15CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACGCGCAAACAGATTAACACTCCGCCTAAGTCTGCCGCACGC-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0574 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/8ATACCAGCTTATTCAATT-AGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.PA#2/8 competes with IgG or IgG-Fc for binding to Protein A.11Bead-based Binding Assay1.06 ±0.2 μMDetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0575 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/22ATACCAGCTTATTCAATT-GCAGTACTGATGAGTGTAGCCGTATGATTATCGTTTGTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0576 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/34ATACCAGCTTATTCAATT-CCCCAACGAGTCGATATGTAGCCCACACTCTGATTCGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0577 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/11ATACCAGCTTATTCAATT-GGAGACGACAAACTATTACGTACTACGGCATGCACTTGGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0578 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/3ATACCAGCTTATTCAATT-CGACAAGTGGGCATTACGATTCTAGCCCTGATTATGTTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0579 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/6ATACCAGCTTATTCAATT-ACCGATCACTAGCCGACTAATTGGTTTCCGATCGCAGTCC-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0580 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#14/82ATACCAGCTTATTCAATT-CCACAACCGAACTCGTAAGACGTATGTAGCCGCCAACTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0581 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/52ATACCAGCTTATTCAATT-ACGCATTGGAGCCCGAAACTGATTCATTGAGCCTACCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0582 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#2/14ATACCAGCTTATTCAATT-ACGACCGTAGACGACTTACACTGATGTTGCGCATTTCTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0583 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#4/31ATACCAGCTTATTCAATT-CGATGACGACTGTAGCCGCAATACGCCCCTGTTACGTTGT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0584 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/41ATACCAGCTTATTCAATT-GGACGCCGACTAACTTTACGTGGTTCTCCTACCGCCTAACC-ACAATCGTAATCAGTTAG775'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0585 262217302015In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein APA#6/43ATACCAGCTTATTCAATT-ACGAAATGTAGCCGATCCTGATTACTCTCTGTCAGCTTGG-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838)Staphylococcus aureus Protein A (SpA)Identify aptamers against Protein A.N/A11Bead-based Binding AssayN/ADetectionFluMag-SELEX5'-Fluorescein Labeled and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0586 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-1CGGATCCATGGGCACTATTTATATCAA-TATGCCAGCATTTCGCCAACGGTGGTCATACAGTGTGAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0587 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-2CGGATCCATGGGCACTATTTATATCAA-GGGTATCCCACTCGGACGTTCATACCTGGCTGGTTGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0588 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-3CGGATCCATGGGCACTATTTATATCAA-AGCGCGTTTTGGACATGCGCTAGCTTCAATTTGAGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0589 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-4CGGATCCATGGGCACTATTTATATCAA-GCCAATGCACTCTGGTTATTCCCCTAAACATCCCGGAAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0590 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-5CGGATCCATGGGCACTATTTATATCAA-CGTTTGGGGCTGTGTTGCAAGACCGTACGTTGCCCCCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0591 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-6CGGATCCATGGGCACTATTTATATCAA-TGCAACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0592 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-7CGGATCCATGGGCACTATTTATATCAA-TGGTACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0593 252698122015Screening and development of DNA aptamers specific to several oral pathogensAPG-8CGGATCCATGGGCACTATTTATATCAA-TTACAGTATCCTCCCACGTTGGTTCGTGTCTTACGGAAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 APG aptamers were effectively bound to P. gingivalis cells; 3 out of 8 aptamers showed slight binding to the lysates of T. denticola pellet.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0594 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-1CGGATCCATGGGCACTATTTATATCAA-CCCGTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0595 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-2CGGATCCATGGGCACTATTTATATCAA-GGCTAGGCCCCGGATCACCACTTTCCCTGCTTGATGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0596 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-3CGGATCCATGGGCACTATTTATATCAA-CCTTACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0597 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-4CGGATCCATGGGCACTATTTATATCAA-GGCGTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0598 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-5CGGATCCATGGGCACTATTTATATCAA-CTGCACGTGGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0599 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-6CGGATCCATGGGCACTATTTATATCAA-GACTACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0600 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-7CGGATCCATGGGCACTATTTATATCAA-GCCATTCGTCACTGAGTTGCTCTAGTGCCGTGTGCTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0601 252698122015Screening and development of DNA aptamers specific to several oral pathogensATD-8CGGATCCATGGGCACTATTTATATCAA-CTAAACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNATreponema Denticola (ATCC 33521)Whole cellScreen and develop aptamers specific to oral pathogens.All 8 ATD aptamers bound to the cell lysates of T. denticola, some showed cross-binding to P. gingivalis and E.coli, but not to S. mutans.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0602 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-1CGGATCCATGGGCACTATTTATATCAA-GCATCGGTCCTGAAGTTGCTCTAGTGCCCGTGTGCTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0603 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-2CGGATCCATGGGCACTATTTATATCAA-GGGCTAGCCCCGGATCACCACTTTCCCTGCTTGATGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0604 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-3CGGATCCATGGGCACTATTTATATCAA-TGTAACGGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0605 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-4CGGATCCATGGGCACTATTTATATCAA-TGGCACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0606 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-5CGGATCCATGGGCACTATTTATATCAA-CCTAACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0607 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-6CGGATCCATGGGCACTATTTATATCAA-CGGTTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0608 252698122015Screening and development of DNA aptamers specific to several oral pathogensASM-7CGGATCCATGGGCACTATTTATATCAA-GTGTTTCGCGCTATTTCCGTACAACCCGCGGACGCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Mutans (ATCC 25175)Whole cellScreen and develop aptamers specific to oral pathogens.ASM showed selective binding to S. mutans cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0609 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-1CGGATCCATGGGCACTATTTATATCAA-CCTCACATAGTGACGATGTGGTTTGGTACCTCTATGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0610 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-2CGGATCCATGGGCACTATTTATATCAA-CCGTGGATGTACGAAATTAACCGCACACCTAGCTACCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0611 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-3CGGATCCATGGGCACTATTTATATCAA-CGCATGCTTCTCGGGATACGGGCGGCTCGATAGAGTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0612 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-4CGGATCCATGGGCACTATTTATATCAA-GCTTACTACGTTTGCCTCAGTGTGTTCCGGTCTTAACAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0613 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-5CGGATCCATGGGCACTATTTATATCAA-CCCATACCTTTTTCGATAGTAAGTGCCATGCCCATCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0614 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-6CGGATCCATGGGCACTATTTATATCAA-GCCTAGAACCCCCTGTCTAGTCAAGTAGTGCGAGTGCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0615 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-7CGGATCCATGGGCACTATTTATATCAA-GCCTTACCGCTCTATCACTCGTCTGTTGCCACTCAGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0616 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-8CGGATCCATGGGCACTATTTATATCAA-TGCGCCGATAGTTTCGATCATGATGCATCTGGCTGTCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0617 252698122015Screening and development of DNA aptamers specific to several oral pathogensASO-9CGGATCCATGGGCACTATTTATATCAA-CGGTAGTACTACTGGAGACAACTTCGCATACTTTAGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Oralis (ATCC 10557)Whole cellScreen and develop aptamers specific to oral pathogens.ASO showed selective binding to S.oralis cell lysates without cross-reaction to the cell lysates of P. gingivalis or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0618 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-1CGGATCCATGGGCACTATTTATATCAA-TTGCTACAGGTATTGCACAACCTCGTGTGGTGTCCGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0619 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-2CGGATCCATGGGCACTATTTATATCAA-CGGTAGTACTACTGGAGACAACTTCGCATACTTTAGTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0620 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-3CGGATCCATGGGCACTATTTATATCAA-GCTGGCAACTGGATGCACTCGTCCTCAGCCTCGGTCAAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0621 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-4CGGATCCATGGGCACTATTTATATCAA-GCCCGTGAGACTATACCCTATGCACTAGTTGCGTAATAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0622 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-5CGGATCCATGGGCACTATTTATATCAA-GCTCGCGTCTGAGGGAAGTTACGTTATTTCGCTTGTAAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0623 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-6CGGATCCATGGGCACTATTTATATCAA-CCGGACTTTAAGCGGCTTCTCGTGGCTGGGTAATCCTAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0624 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-7CGGATCCATGGGCACTATTTATATCAA-CCTGACCGATGTTGTATTAATGGCTCCGGGTCTTATGAG-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0625 252698122015Screening and development of DNA aptamers specific to several oral pathogensASS-8CGGATCCATGGGCACTATTTATATCAA-CGAGGGGTTGGTTTGTGGTGTTGCCGCCACTACAGCCAA-AATGTCGTTGGTGGCCC835'-CGGATCCATGGGCACTATTTATATCAA-N40-AATGTCGTTGGTGGCCC-3'ssDNAStreptococcus Sanguis (ATCC 10556)Whole cellScreen and develop aptamers specific to oral pathogens.Several ASS aptamers showed cross-reactions with lysates of P. gingivalis and/or T. denticola.N/AModified Western Blot AssayN/ADetectionWhole Cell-SELEXDIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0626 255627422015Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of staphylococcus aureusPrimary anti-S.aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDual-aptamer-based sandwich immunosensor (Apt/S.aureus/apt-AgNP) for the detection of S. aureus.Display a dynamic range from 10 to 1×10(6) cfu/mL with a low detection limit of 1.0 cfu/mL.2N/A35 nMBiosensorWhole Cell-SELEX5'-Biotinylated (Biotin-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0627 255627422015Aptamer-conjugated silver nanoparticles for electrochemical dual-aptamer-based sandwich detection of staphylococcus aureusSecondary anti-S.aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDual-aptamer-based sandwich immunosensor (Apt/S.aureus/apt-AgNP) for the detection of S. aureus.Display a dynamic range from 10 to 1×10(6) cfu/mL with a low detection limit of 1.0 cfu/mL.2N/A129 nMBiosensorWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0628 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA1UUCAGGGACCAGUGUUUGCCUUGUGCCAGUC315'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.IC50 value is higher than 15 μM.8Ribogreen dye-based fluorescence intensity assay108 ± 33.4 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0629 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA2CCCAUAGUGAGUCGUAUUAAUUG235'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.IC50 value is higher than 15 μM.8Ribogreen dye-based fluorescence intensity assay296 ± 57.1 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0630 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA3GCGACCCUAUAGUGAGUCGUAUUAAUGCGC305'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.IC50 value is higher than 15 μM.8Ribogreen dye-based fluorescence intensity assay388 ± 79.2 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0631 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA4GCGCCCCUAUAUGAGUCGUAUUAAUUGCGC305'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.IC50 value is higher than 15 μM.8Ribogreen dye-based fluorescence intensity assay105 ± 10.9 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0632 258413812015Development of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationABA5GCGCCCCCAUAGUGAGUCGUAUUAAUACGC305'-GCGCGGATCCCGCGC-N30-CGCGCGA AGCTTGCG-3'ssRNABacillus Anthracis (BA)Anthrax toxin (ATR/TEM8 Von Willebrand factor type A (VWA) domain)Identify aptamers that bind to the anthrax toxin receptor (ATR), which blocks the binding of PA to its receptor, in order to neutralize anthrax toxin.ABA 5 rescued cells to at least 50% maximum cell viability, with an IC50 value of 5 μM.8Ribogreen dye-based fluorescence intensity assay205 ± 69.8 nMTherapeuticsSELEXN/AN/AN/AN/AN/AN/A
ABdb_0633 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisMA1GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.Sandwich ELISA based on MA2 and MA1 display a linear range of 8 × 10(4) to 128 × 10(4) CFU with the detection limit of 1 × 10(4) CFU.10Bio-Layer Interferometry (BLI)12.02 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0634 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisMA2GGGAGCTCAGAATAAACGCTCAA-TATCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.Sandwich ELISA based on MA2 and MA1 display a linear range of 8 × 10(4) to 128 × 10(4) CFU with the detection limit of 1 × 10(4) CFU.10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0635 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE6-15GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACCGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0636 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-3GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACCGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0637 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-4GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACTGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0638 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE7-12GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAGTAGACTGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0639 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-5GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGTCTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0640 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE9-16GGGAGCTCAGAATAAACGCTCAA-CGGGCTAGTCAATAGACTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0641 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP6-5GGGAGCTCAGAATAAACGCTCAA-TATCCCTATTCCGCTCCATGCTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0642 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP10-4GGGAGCTCAGAATAAACGCTCAA-TACCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0643 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP10-8GGGAGCTCAGAATAAACGCTCAA-CATCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0644 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.06TGTACCGTCTGAGCGATTCGTAC-GATTACTATAATTTCCTATCGTCCGACCGCCGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.Display a sensitive detection of 200 nM alpha toxin.12Surface Plasmon Resonance (SPR)93.7 ± 7.0 nMDetectionSELEX5'-Amidation (NH₂-(CH2)6) or 5'-FAM LabeledN/AN/AN/AAverage half-life of ssDNA MREs in serum is about one hour.N/A
ABdb_0645 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.26TGTACCGTCTGAGCGATTCGTAC-CCTTGCCGATGCCTTTACGGTCTAGTTTGGATGT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0646 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.01TGTACCGTCTGAGCGATTCGTAC-TCGGGCGATGATACTTAGCACGGTCTAGGTCAAA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0647 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.20TGTACCGTCTGAGCGATTCGTAC-TAGCGGCAGAGTAGCACTCTATAGGTCGATGTTT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0648 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.02TGTACCGTCTGAGCGATTCGTAC-CGTGTCCTATTTTCTTCTCTGTTAACTCTCGTC-AGCCAGTCAGTGTTAAGGAGTGC795'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0649 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.06TGTACCGTCTGAGCGATTCGTAC-GATTACTATAATTTCTTATCGTCCGACCGCCGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0650 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.39TGTACCGTCTGAGCGATTCGTAC-TTTGATCTCGTGTGTCTAGTTGCGGCGGATTGTC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0651 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.10TGTACCGTCTGAGCGATTCGTAC-GGTCAACCTCACCGACTGCCGACCGTTTAATTCG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0652 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.43TGTACCGTCTGAGCGATTCGTAC-CGTCATTGCCTCGTAGTATTCTTATAGTCGGTAG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0653 256331022015In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serumR12.44TGTACCGTCTGAGCGATTCGTAC-TCCCGAAAGCGCGTCAGCCTGGGAGGTTATGCGG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAStaphylococcus aureus (S. aureus)Molecular recognition element (MRE) targeting alpha toxinIdentify aptamer against MRE and develop modified sandwich ELISA to detect alpha toxin in undiluted human serum samples.N/A12Surface Plasmon Resonance (SPR)N/ADetectionSELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0654 255909732015Analytical bioconjugates, aptamers, enable specific quantitative detection of Listeria monocytogenesLMCA2ATACCAGCTTATTCAATT-CCAAAAGCGCACCCATATATGTTCTATGTCCCCCACCTCG-AGATTGCACTTACTATCT765'-ATACCAGCTTATTCAATT-N40-AGATTGCACTTACTATCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Listeriolysin O (LLO) proteinDeveloped an aptamer-based sandwich assay (ABSA) platform to distinguish L. monocytogenes from other Listeria species and other bacterial genera.Exhibited a linear response from 20 to 2×106 CFU per mL with a LOD of 20 CFU/mL.10Surface Plasmon Resonance (SPR)2.01×10(-12) MBiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0655 255909732015Analytical bioconjugates, aptamers, enable specific quantitative detection of Listeria monocytogenesLMCA26ATACCAGCTTATTCAATT-CGATGGGGATTACTTATCATGAGAAACCAGCAGCATTTG-AGATTGCACTTACTATCT755'-ATACCAGCTTATTCAATT-N40-AGATTGCACTTACTATCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Listeriolysin O (LLO) proteinDeveloped an aptamer-based sandwich assay (ABSA) platform to distinguish L. monocytogenes from other Listeria species and other bacterial genera.Exhibited a linear response from 20 to 2×106 CFU per mL with a LOD of 20 CFU/mL.10Surface Plasmon Resonance (SPR)1.56×10(-10) MBiosensorWhole Cell-SELEXCyanine5 (Cy5) LabeledN/AN/ABest CandidateN/AN/A
ABdb_0656 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.33TGTACCGTCTGAGCGATTCGTAC-CATAGGGTGCTTTTCAAGGCCACACGTTAGTGTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.100 nM or 6.6 μg/mL of Exotoxin A in human serum was detected.14Surface Plasmon Resonance (SPR)4.2 and 4.5 μMDiagnosticDecoy-SELEX5'-Amidation (NH₂-(CH2)6) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0657 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.02TGTACCGTCTGAGCGATTCGTAC-TACGCCACACGTGGTGAGGGATTCGATCGCTTGA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0658 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.20TGTACCGTCTGAGCGATTCGTAC-TGCTATTCATCACCACTCTAGAGCCACTTTTAAA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0659 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.22TGTACCGTCTGAGCGATTCGTAC-TGGGCGGCGAGCCACCCGGCAATTTAGTACAGGC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0660 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.36TGTACCGTCTGAGCGATTCGTAC-AAAGCATCCAGCCGGTATGTGCCAGAGTCTCTGA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0661 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.38TGTACCGTCTGAGCGATTCGTAC-AAATGTGAGTGGCCAGGCATCAGGTACGTCGGTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0662 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.03TGTACCGTCTGAGCGATTCGTAC-GGATAGGTGCCTCTGCTTCATCATGTTGAACTTA-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0663 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.13TGTACCGTCTGAGCGATTCGTAC-AGTTTCACCAGTCGCCTGTTAGCCGTGATATACG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0664 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.27TGTACCGTCTGAGCGATTCGTAC-TGTCAATATTACGTTGCTCTTAGGTTCACCATCT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0665 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.28TGTACCGTCTGAGCGATTCGTAC-TTGTGATTCAAATAGGCGTGTTGGTGTGAGACCT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0666 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.23TGTACCGTCTGAGCGATTCGTAC-CGTGGATCATGCTTCGCGTCGGTTTATAGGTTCC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0667 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.39TGTACCGTCTGAGCGATTCGTAC-AGAAGAGCATCGGTAACTTCCATAGGAGATGGGG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0668 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.06TGTACCGTCTGAGCGATTCGTAC-CATCCGAGGGTATTGTATGCGTATATCCTAGTCG-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0669 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.10TGTACCGTCTGAGCGATTCGTAC-CAAGTTCCTCATGGAGGGTGCTCAGAGCTTAGAC-AGCCAGTCAGTGTTAAGGAGTAA805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0670 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.34TGTACCGTCTGAGCGATTCGTAC-AGGGGGATTCCTAGGGCCCGGCCCAACGCTGTTT-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0671 266360982015Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human SerumR14.42TGTACCGTCTGAGCGATTCGTAC-CAAGACCCTTGAATCACGGGTAGGGTCTCGTAAC-AGCCAGTCAGTGTTAAGGAGTGC805'-TGTACCGTCTGAGCGATTCGTAC-34N-AGCCAGTCAGTGTTAAGGAGTGC-3'ssDNAPseudomonas AeruginosaExotoxin AIdentify ssDNA MREs against Exotoxin A and develop a sandwich ELISA for its detection in human serum.N/A14N/AN/ADiagnosticDecoy-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0713 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp1TGAGCCCAAGCCCTGGTATG-TTCTTCCCTTTTATTAGTCCTGTATTCCTCTACTGTTGCC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.With sandwich Sp1 and Sp20, the aptamer assay achieved a concentration range for S. sonnei of 10(2) cfu/mL to 10(7) cfu/mL and a detection limit of 30 cfu/mL.12Flow Cytometry5.98 ± 0.835 nMDetectionWhole Cell-SELEX5'-FAM Labeled and BiotinylatedN/AN/AN/AN/AN/A
ABdb_0714 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp13TGAGCCCAAGCCCTGGTATG-TATCCGATTTTATCTGTCCAATATTCCCTGTGTCTACCTT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry97.38 ± 36.2 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0715 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp16TGAGCCCAAGCCCTGGTATG-TATCTTCACTTGACCTTTACCGCAATCGTCCATGTATCTG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry682.2 ± 125 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0716 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp19TGAGCCCAAGCCCTGGTATG-TTCTACCCAACTGCTTTAATAGTCACTGTCATAGTTCCAC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0717 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp20TGAGCCCAAGCCCTGGTATG-TCGTCGATTATTGTTTCAGTTCAGTTCCCCCGCGTTCCGA-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.With sandwich Sp1 and Sp20, the aptamer assay achieved a concentration range for S. sonnei of 10(2) cfu/mL to 10(7) cfu/mL and a detection limit of 30 cfu/mL.12Flow Cytometry14.32 ± 2.19 nMDetectionWhole Cell-SELEX5'-FAM Labeled and HEX (hexachlorofluorescein) LabeledN/AN/AN/AN/AN/A
ABdb_0718 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp22TGAGCCCAAGCCCTGGTATG-CCTACCTTAGTCCGTTTCGCTGTGTTTGTCCAATATTCCT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry4.98 ± 0.357 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0719 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp23TGAGCCCAAGCCCTGGTATG-CTTCACTTGACCCTTATCGCTTCTAACACAACCGCTTTTT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry5.735 ± 0.478 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0720 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp28TGAGCCCAAGCCCTGGTATG-TATTCCCCCTTATTTTAGCCATTTTGTTACATCTTCCGTC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry72.89 ± 22.16 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0721 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp29TGAGCCCAAGCCCTGGTATG-ATATTCGTTCTTTTCGCCTTAACTTCTCGTGTTTCCTTAC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry47.83 ± 9.7 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0731 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL26TAGCTCACTCATTAGGCA-CATTTGTGGCACCAAATTTGAATTAATCAAGACAGTGTGGT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.SAL 26 can detect 10(2) CFU/mL in the gold nanoparticle-based colourimetric assay and has a limit of detection (LOD) of 10(3) CFU/mL in the aptamer sedimentation assay.10Fluorescence Spectroscopy123 ± 23 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0732 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL33TAGCTCACTCATTAGGCA-CTCGTATTGAGGAGCTCGTATTATTTAATTCACACTGTGCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence Spectroscopy221 ± 72 nMBiosensorWhole Cell-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0733 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL28TAGCTCACTCATTAGGCA-CTCTGTCGTCAACAGTCTAACTTGTAGAATTGATGTTACTG-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.Limit of detection (LOD) of 10(3)CFU/mL in the aptamer sedimentation assay.10Fluorescence Spectroscopy195 ± 46 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0734 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL43TAGCTCACTCATTAGGCA-CGAGACCACCGGCAACCTTTCACAAAGGGGAGGCTAA-GCATAGTTAAGCCAGCC725'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence Spectroscopy294 ± 112 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0735 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL21TAGCTCACTCATTAGGCA-CAGCAACTCGCTTTGATACCGGTAGAGTTTGTATTGCTCAA-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0736 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL37TAGCTCACTCATTAGGCA-CTTAGTTCAGACGCGAGTATTATCGGCGTCCAAGCAAGGG-GCATAGTTAAGCCAGCC755'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0737 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL20TAGCTCACTCATTAGGCA-CATATTCAGCAGTTGTATGATAGCGGGTCGCGAGTGTGTAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0738 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL40TAGCTCACTCATTAGGCA-CTGCATTACATATATGGCAGTGGGTTAACCTGGATTGAGGA-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence Spectroscopy204 ± 73 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0739 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL16TAGCTCACTCATTAGGCA-CCCGATTGTACTAATGAAGATGGACACTGAGAGGGGGATGG-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0740 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL11TAGCTCACTCATTAGGCA-CTCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.Limit of detection (LOD) of 10(3)CFU/mL in the aptamer sedimentation assay.10Fluorescence Spectroscopy184 ± 43 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0741 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL45TAGCTCACTCATTAGGCA-CCGTGCCAACTGAGGGATACAGGGGGAGTGTCGGTCTACCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence Spectroscopy294 ± 133 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0742 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL23TAGCTCACTCATTAGGCA-CCTCACAGCCCTTCTTCCCGTATTTGTAGACTAGTTACATT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0743 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL48TAGCTCACTCATTAGGCA-CCACACTGTCTTGATTTTGGATTTGTCGGTGCTGACCTGTG-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0744 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL9TAGCTCACTCATTAGGCA-CTGCGAGTGAACTCCTCCTTTTGTATTTAGTGTGCGGATCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0745 270704142016Selection and Characterization of Aptamers Using a Modified Whole Cell Bacterium SELEX for the Detection of Salmonella enterica Serovar TyphimuriumSAL29TAGCTCACTCATTAGGCA-CATGTGGAGTGCGAACTGTCTGGTCTTATCGGGCTCGCTGT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC 3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify an aptamer and develop a nanogold-based colourimetric method or sedimentation assay for detecting Salmonella enterica serovar Typhimurium cells.N/A10Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0746 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB5CAGTCCAGGACAGATTCGCGAG-CCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATC-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.LOD was 7.9 × 10(3) CFU/mL and a linear range from 7.9 × 10(2) to 7.9 × 10(6) CFU/mL of S. typhimurium with less than 1 h.10Quartz Crystal Microbalance (QCM)58.5 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0747 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumA24CAGTCCAGGACAGATTCGCGAG-GCACAATCCACCTCTCACCGCACGCCACGCACTGCCTCTGTCCCG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)19.63 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0748 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB30AATCGCTGAATGAAATCCACGTG-GGAAGTGTGTGGGTGACCAGAGGTGTGGTGATGGGATTGTCGTA-CCTCGCGAATCTGTCCTGGACTG905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)51.02 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0749 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB31CAGTCCAGGACAGATTCGCGAG-CCGCACCGCCCTATCCTCGTGTCCCTGCCCAACAGCGCCGCCCGC-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0750 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumA37CAGTCCAGGACAGATTCGCGAG-GTGGGGCGGGAAAGTGTGTGTAGGTGGTGGCGTTGTCACGGCGGG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0751 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB16CAGTCCAGGACAGATTCGCGAG-GGGCTGTGTGAGAAGTGGGTGTGGTGGTGGATATGCAACGTTGTA-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0752 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB21CAGTCCAGGACAGATTCGCGAG-GTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0753 275295082016A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against TuberculosisBM2GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CCCCATGAACTAGGCTCCACAATGAGTTTGG-GGGTCAATGCGTCATA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Bovis (bacillus Calmette–Guérin (BCG))Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.Ki of BM2 was estimated to be 10.34 ± 0.27 nM, with a LOD of 1 × 10^4 cfu/100 μL of BCG.10Enzyme-Linked Oligonucleotide Assay (ELONA)8.59 ± 1.23 nMTherapeutics/ImmunmodulatorySELEXBiotinylatedBM2 was demonstrated to be safe with low toxicity based on in vitro and in vivo toxicity analyses.BM2–BCG complex could be detected and remained stable for at least 30 days in vivo.N/AN/AN/A
ABdb_0754 275295082016A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against TuberculosisBM1GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CGGTCGTAGACCGGGAGTTGTTGTTAGTTCA-GGGTCAATGCGTCATA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Bovis (bacillus Calmette–Guérin (BCG))Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.N/A10Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0755 275295082016A Single ssDNA Aptamer Binding to Mannose-Capped Lipoarabinomannan of Bacillus Calmette-Guérin Enhances Immunoprotective Effect against TuberculosisBM4GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-CGCGCAGCGGGTCGACTTCATTCCTCACCAT-GGGTCAATGCGTCATA895'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N31-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Bovis (bacillus Calmette–Guérin (BCG))Mannose-capped lipoarabinomannan (ManLAM) epitopeIdentified aptamers against ManLAM and served as an immune enhancer for BCG against M. tb H37Rv infection via blocking MR binding, triggering ManLAM–CD44 signalling, and enhancing M1 macrophage and Th1 activation via cellular surface CD44 in vitro and in vivo.N/A10Enzyme-Linked Oligonucleotide Assay (ELONA)N/ATherapeutics/ImmunmodulatorySELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0756 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS3ATACCAGCTTATTCAATT-CCCGCCTCTGAGCATTATTAATGTTATACCTTACGGCTGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.Inhibited PBMCs proliferation with an inhibition rate of 84% and reduced IFN-γ, TNF-α, IL-2, and IL-6 secretions by 89%, 87%, 73%, 69%, respectively. PEGS3 allowed 79% of the mice to survive the lethal challenge.9Enzyme-Linked Aptamer Assay (ELAA)36.93 ± 7.29 nMTherapeutics/ImmunmodulatorySELEX5′-Polyethylene glycol (PEG) with 5'-Amidation (NH₂) and 3’-Inverted Thymidine (3'-idT) and 5'-Biotinylated or 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0757 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS6ATACCAGCTTATTCAATT-GCCGCGGAATTGTGGCTTGGTGTCGTGGTCAGGGGCTGG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.N/A9Enzyme-Linked Aptamer Assay (ELAA)N/ATherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0758 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS12ATACCAGCTTATTCAATT-GCCACTGTGGACTTGTTATTCGTCTTTGCCGTCTAATTCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.Inhibited PBMCs proliferation with an inhibition rate of 84%.9Enzyme-Linked Aptamer Assay (ELAA)79.35 ± 12.09 nMTherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0759 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS23ATACCAGCTTATTCAATT-GCAACAATGTTGTGTCAGCAAGTTGGTTGGCGGTGTATTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.N/A9Enzyme-Linked Aptamer Assay (ELAA)N/ATherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0761 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-52CATACGATTTAGGTGACACTATAG-CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.P12-52 bound strongly to all three Gram-positive bacteria tested (S. aureus, E. faecalis and E. freudini).12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)11.97 ± 2.94 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0762 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-31CATACGATTTAGGTGACACTATAG-CCCTCCGGGGGGGGGGGTCATCGGGATACCTGGTAAGGATA-ATTTCTCCTACTGGGATAGGTGGA895'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.The binding of P12-31 was 10-fold stronger than that of the random library.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)87.03 ± 17.32 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0763 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-55CATACGATTTAGGTGACACTATAG-CCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.The binding of P12-55 to E. coli cells was more than 100-fold stronger than that of the random library.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)161.0 ± 34.74 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0764 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-17CATACGATTTAGGTGACACTATAG-GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.P12-17 could only bind to E. faecalis. The binding of P12-17 was 10-fold stronger than that of the random library.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)56.33 ± 15.87 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0765 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-11CATACGATTTAGGTGACACTATAG-CCCTCCGGGGGGGTCATCGGGATACCTGGTAAGGATA-ATTTCTCCTACTGGGATAGGTGGA855'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0766 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-21CATACGATTTAGGTGACACTATAG-CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0767 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-9CATACGATTTAGGTGACACTATAG-TGCGGGCGGAGGACACGGACCCGTATGGGAGCAATGCACG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0768 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-15CATACGATTTAGGTGACACTATAG-GGCCACCCACAGGCACTCCGCTCATGAATCGTGGAGTCGG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0769 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-30CATACGATTTAGGTGACACTATAG-CACCACGGACACGATCCCAAGCTAGGAGGTGCGGCGGGGT-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0770 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-48CATACGATTTAGGTGACACTATAG-TGGCACAGCACGTCGCACGGTCCCCGGGAGGTGTTCACTG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0771 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-51CATACGATTTAGGTGACACTATAG-TCGCGAGTGCGTGTACGCCACACATCACAAAAGGGGTGTG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0772 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-66CATACGATTTAGGTGACACTATAG-GGCAACATTCAGACATACCAGTACCCACTCGGACTTCCCG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0776 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc3 (31) -TruncatedUUCAAUUGCACGAAUUUGCUGUGUUUUUGGG315'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.Ec3(31)-biotin-streptavidin magnetic separation has LOD of 1.3 × 10(6) CFU/ml, Optical analytic technique has LOD of 5 × 10(4) CFU/ml, and Electrochemical Impedance Spectroscopy (EIS) has LOD of 2 × 10(4) CFU/mL of E. coli cells.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)225 nMBiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-Biotinylated or Thiolated or Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0777 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc3AUACCAGCUUAUUCAAUUGCACGAAUUUGCUGUGUUUUUGGGGGGGUCGGGGAGUAUAAGAUAGUAAGUGCAAUCU765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0778 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc2AUACCAGCUUAUUCAAUUCUUCUUUGAUCUGCUCGUAUCAUGGGACGGGAGGGUAUAGAUAGUAAGUGCAAUCU745'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0779 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc5AUACCAGCUUAUUCAAUUCGAAUUUAAGUUUCUUUUCUGGUAUAGUGGUUGGGUGGGUAAGAUAGUAAGUGCAAUCU775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0780 281211692016Development of a DNA aptamer that binds specifically to group A Streptococcus serotype M312L18AGCCTGTTGTGAGCCTCCTAAC-TCCTCGAGGGGGGGGGGATGAAAAGGAAAACGCAACAA-CATGCTTATTCTTGTCTCC785'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAGroup A Streptococcus (GAS) serotype M3Whole cellIdentify aptamers against S. pyogenes serotype M3.74% efficiency toward the S. pyogenes serotype M3.12Flow Cytometry7.47 ± 1.72 pMDetectionWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0781 281211692016Development of a DNA aptamer that binds specifically to group A Streptococcus serotype M312L12AGCCTGTTGTGAGCCTCCTAAC-GAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA-CATGCTTATTCTTGTCTCC785'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAGroup A Streptococcus (GAS) serotype M3Whole cellIdentify aptamers against S. pyogenes serotype M3.N/A12Flow Cytometry8.25 ± 1.43 pMDetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0782 272348802016Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolatesAci49GCCTGTTGTGAGCCTCCTAAC-TACATGGTCAACCAAATTCTTGCAAATTCTGCATTCCTACTGT-CATGCTTATTCTTGTCTCC835'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAAcinetobacter Baumannii (ATCC 19606)Whole cellIdentify aptamers against and develop a sandwich enzyme-linked aptamer sorbent assay (ELASA) for the rapid detection of A. baumannii from clinical isolates.Display detection limit of 10(3) CFU/ml, and the sensitivity of the test toward the clinical isolates was 95.47%.12Flow Cytometry7.547 ± 1.353 pMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0783 272348802016Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolatesAci55GCCTGTTGTGAGCCTCCTAAC-GTTACATGGTCAACCAAATTCTTGCAAATTCTGCATTCCT-CATGCTTATTCTTGTCTCC805'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAAcinetobacter Baumannii (ATCC 19606)Whole cellIdentify aptamers against and develop a sandwich enzyme-linked aptamer sorbent assay (ELASA) for the rapid detection of A. baumannii from clinical isolates.N/A12Flow Cytometry10.70 ± 2:561 pMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0796 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC2TGCAGGATCCGGTATCCGTGGACGGTGTGCAGGATCCGGTATCCGTGGGCACGAGAATTCCTCCGTTGCG705'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.LOD in the minimum quantity of 5 ng SEB per 100 µl of human serum.12Enzyme-Linked Immunosorbent Assay (ELISA)2.3 × 10(−11) MBiosensorSELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0797 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC3TGCAGGATCCGGTATCCGTGCACACACACCCAACAACCAGCTGCCGCACCGGAGGAATTCTCGT645'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0798 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC7TGCAGGATCCGGTATCCGTGGGCGGGGCCATGCAGGATCCGGTATCCGTGGGCCGCAACGGAGGAATCTCGT725'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0799 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC8CCGTGGCCGCAACGGAGGAATTCTCGT275'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0800 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC9ACGAGAATTCCTCCGTTGCGGCACAGTTGGGCAGGAACCTGTGGGGCGTGCGCAACGGAGGAATTCTCGT705'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0801 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC10ACGAGAATTCCTCCGTTGCGGCCCACGGATACC335'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0802 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC11TGCAGGATCCGGTATCCGTGCGCAACGGAGGAATTCTCGT405'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0803 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC12ACGAGAATTCCTCCGTTGCGGCCCACGGATACAGGATCCTGCATGCCTGTCCACGGATACCGGATCCTCA705'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0804 270913272016Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatographyHedC17TGCAGGATCCGGTATCCGTGCCAAACACACCAAAAGCCCCCCCAACCCACAACCACGTCC605'-TGCAGGATCCGGTATCCGTG-N40-CGCAACGGAGGAATTCTCGT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Identify aptamers that bind to and detect SEB in infected serum samples.N/A12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0814 267685822016DNA aptamers for the detection of Haemophilus influenzae type b by cell SELEXClone 63GCCTGTTGTGAGCCTCCTAAC-AGGAGGCGAACGGGCAGGTTCTTGGGGAGACAAGAATAAG-CATGCTTATTCTTGTCTCC805'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAHaemophilus Influenzae type b (Hib) (ATCC 10211)Whole cellIdentify an aptamer against Hib for the detection of H. influenzae.Clone 63 could detect Hib in patients’ CSF samples at 60 CFU/mL.7Flow Cytometry28.46 pMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0815 267685822016DNA aptamers for the detection of Haemophilus influenzae type b by cell SELEXClone 2, 42, 45GCCTGTTGTGAGCCTCCTAAC-GCCAGGTGTTTTGGCGGTAGGGGTGAAACAACAGGGGG-CATGCTTATTCTTGTCTCC785'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAHaemophilus Influenzae type b (Hib) (ATCC 10211)Whole cellIdentify an aptamer against Hib for the detection of H. influenzae.N/A7Flow Cytometry47.10 pMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0823 280693772017Development of an aptamer-ampicillin conjugate for treating biofilmsAptamer 3GGGAGCTCAGAATAAACGCTCAA-GGCAGGACAACAGCGTGTAGTATCAGCTTACGGTG-TTCGACATGAGGCCCGGATC78N/AssDNASalmonella CholeraesuisFlagellaAptamer-ampicillin conjugate (Apt3-Amp) to inhibit biofilm formation.Biofilms containing the conjugate had the lowest survival ratio (37.22 ± 1.12%) compared with control conjugates.14Fluorescence Spectroscopy41 ± 2 nMTherapeuticsWhole Cell-SELEX3'-Amidation (NH₂) and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0824 280693772017Development of an aptamer-ampicillin conjugate for treating biofilmsAptamer 1GGGAGCTCAGAATAAACGCTCAA-GGCCAGCAGCGAGTGTGGAGTATTGTGTTGTGGTG-TTCGACATGAGGCCCGGATC78N/AssDNASalmonella CholeraesuisFlagellaAptamer-ampicillin conjugate (Apt3-Amp) to inhibit biofilm formation.N/A14Fluorescence Spectroscopy50 ± 2 nMTherapeuticsWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0825 280693772017Development of an aptamer-ampicillin conjugate for treating biofilmsAptamer 2GGGAGCTCAGAATAAACGCTCAA-GGGCAGGTGTTATGTGTACTGCTACAGTGTGGTTG-TTCGACATGAGGCCCGGATC78N/AssDNASalmonella CholeraesuisFlagellaAptamer-ampicillin conjugate (Apt3-Amp) to inhibit biofilm formation.N/A14Fluorescence Spectroscopy54 ± 2 nMTherapeuticsWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0826 280693772017Development of an aptamer-ampicillin conjugate for treating biofilmsAptamer 4GGGAGCTCAGAATAAACGCTCAA-GGCACGGCATGTGTGGTATGTGGTGCCTGTACTCG-TTCGACATGAGGCCCGGATC78N/AssDNASalmonella CholeraesuisFlagellaAptamer-ampicillin conjugate (Apt3-Amp) to inhibit biofilm formation.N/A14Fluorescence Spectroscopy53 ± 5 nMTherapeuticsWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0827 286467192017DNA aptamer-based colorimetric detection platform for Salmonella EnteritidisCrn-1AAGGGCTGGCTGGGATGGA-CCCTCCCGAAACGAGCTGTCTCTTAACGGAAGCTAATCTGCC-TCACTCCACGGACCCCACT805'-AAGGGCTGGCTGGGATGGA-N42-TCACTCCACGGACCCCACT-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellIdentify aptamers that bind to and develop a sandwich aptamer-based colorimetric bioassay to detect S. Enteritidis at 4°C.Display a LOD of 10(3) CFU/mL for S. Enteritidis.12Isothermal Titration Calorimetry (ITC)0.971 ± 0.013 µMBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0828 286467192017DNA aptamer-based colorimetric detection platform for Salmonella EnteritidisCrn-2AAGGGCTGGCTGGGATGGA-TGTAAGAAGGGAGGAAAGGACCTAAGACCTGCTATATTGCGA-TCACTCCACGGACCCCACT805'-AAGGGCTGGCTGGGATGGA-N42-TCACTCCACGGACCCCACT-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellIdentify aptamers that bind to and develop a sandwich aptamer-based colorimetric bioassay to detect S. Enteritidis at 4°C.Display a LOD of 10(3) CFU/mL for S. Enteritidis.12Isothermal Titration Calorimetry (ITC)0.309 ± 0.071 µMBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0829 286467192017DNA aptamer-based colorimetric detection platform for Salmonella EnteritidisCrn-3AAGGGCTGGCTGGGATGGA-GGATGGCATTACCTATGCGGTAGATTGCCGACGACCACGAC-TCACTCCACGGACCCCACT795'-AAGGGCTGGCTGGGATGGA-N42-TCACTCCACGGACCCCACT-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cellIdentify aptamers that bind to and develop a sandwich aptamer-based colorimetric bioassay to detect S. Enteritidis at 4°C.N/A12Isothermal Titration Calorimetry (ITC)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0830 295945922017Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assayApt22GCTGTGTGACTCCTGCAA-GCGGGAAGAGGGTAAGGGGAGGGAGGGTAACGCGGAGAAGGCAA-GCAGCTGTATCTTGTCTCC815'-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)Antigen 85 (Ag85A) (FbpA)Identify aptamers that bind to and develop a graphene oxide-based fluorometric assay to detect FbpA.Display LOD in PBS and serum of 1.5 nM and 2.1 nM, respectively, with an analytical linear range from 5 to 200 nM of FbpA.12Flow Cytometry62.95 nMBiosensorMagnetic Bead (MB)-based Protein SELEXATTO647N-labeledN/AN/ABest CandidateN/AN/A
ABdb_0831 295945922017Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assayApt8GCTGTGTGACTCCTGCAA-TCAGGAAAGAACTTAGGGGTGGGAGGAGGGTATAAATACGGAAT-GCAGCTGTATCTTGTCTCC815'-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)Antigen 85 (Ag85A) (FbpA)Identify aptamers that bind to and develop a graphene oxide-based fluorometric assay to detect FbpA.N/A12Flow Cytometry202 nMBiosensorMagnetic Bead (MB)-based Protein SELEXATTO647N-labeledN/AN/AN/AN/AN/A
ABdb_0834 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN08ATGAGAGCGTCGGTGTGGTAACTTAATCCTCTAGTTTTAATTCTAGATACGGCGCATGCACTCTATGTAGGAGGGTGCGGAAGTA855'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry54.9 ± 7.8 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0835 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN27ATGAGAGCGTCGGTGTGGTAACTAGTCTGATTTCTATTTCCTTTAATTAGTCTGCACACATTGCATTGTAGGAGGGTGCGGAAGTA865'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry28.5 ± 4.9 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0836 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN27.SHTAATTAGTCTGCACACATTGCATTGTAGGAGGGTGCGGAAGTA435'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry26.9 ± 5.6 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0837 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN08.SHATGCACTCTATGTAGGAGGGTGCGGA265'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry52.9 ± 10.4 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0838 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN17.SHATCCTCCTATCTATCGGTAGTTGTAGGAGGGTGCGG365'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry29 ± 7.3 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0839 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN21.SHAGAGCGTCGGTGTGGTAACTGTTCAGGAGGATGACATTGTCGCCT455'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry18.1 ± 3.4 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0840 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaSt17Lp21ATCCTCCTAGGTAACTGTTCAGGATGTAGGAGGGT355'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry16.9 ± 3.9 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0841 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaSt21Lp17AAGCGTCGGTGTTCTATCGGTAGTTGACACCGACGCCT385'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry13.5 ± 2 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0842 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaSt08Lp17ATGCACTCTTCTATCGGTAGTTGAGGGTGCGG325'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry12.5 ± 2.4 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0843 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-6CGTGATGATGTTGAGTTG-GGGTGATGGGTGCATGTGATGAAAGGGGTTCGTGCTATGCTGTTTTGTCTAATAATACTAGTCCTTGCCAAGGTTTATTC-CAGTAATGCCAACCAATCT1175'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.66.26% fluorescent cell intensity for E. coli O157 with less cross-reactivity.10Flow Cytometry107.6 ± 67.8 pMDetectionWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0844 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-5CGTGATGATGTTGAGTTG-GAGTTGCGTTGCTGAATGCATGGGCTGTACTAGGTTGGTGCTACTCTGTAATGTCTACTATTACTATTCCATCGCAACGTATACTG-CAGTAATGCCAACCAATCT1235'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.57.41% fluorescent cell intensity for E. coli O157 with less cross-reactivity.10Flow Cytometry120.4 ± 32.2 pMDetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0845 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-1CGTGATGATGTTGAGTTG-GCCGAGATTGAAGCTGGTGACCATTGGCCTTACTGGGATGCTCTTGTGATTACTACTACTAATCGTTGTGAACGTAAATGC-CAGTAATGCCAACCAATCT1185'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.N/A10Flow CytometryN/ADetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0846 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-2CGTGATGATGTTGAGTTG-GTGTTAATCCGTGTATGCCATGAGACCGGTAAGTCCTATTTTCGCTCCTGGAATGGTACAAGCTCATGTCAAGATATACTC-CAGTAATGCCAACCAATCT1185'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.N/A10Flow CytometryN/ADetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0847 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-3CGTGATGATGTTGAGTTG-GGGCAGATCCGTGCACATGATCTTAGCCGTACGTGTTCTGCGGTATAGTCTATTCATACTACCACTTCGCAATGTATATCG-CAGTAATGCCAACCAATCT1185'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.N/A10Flow CytometryN/ADetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0848 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-4CGTGATGATGTTGAGTTG-GTCGAGTACGGATCACTTGGAGATTGGCCATCGAGCTATTCTGTAATGTGTAATCCTACTACACCTTCGCATCGTTAATGC-CAGTAATGCCAACCAATCT1185'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.N/A10Flow CytometryN/ADetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0849 289414532017Development of a DNA Aptamer for Screening Neisseria meningitidis Serogroup B by Cell SELEXK3GCCTGTTGTGAGCCTCCTAACGCCAGGCGTTTTGGCCGTAGGCGTGGGAGACAAGAATAAGCA635'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNANeisseria Meningitidis Serogroup B (ATCC 13090)Whole cellIdentity aptamers bind to and detect N. meningitidis in patients’ CSF samples.Detected 10(2) CFU of CSF isolated N. meningitidis.6Flow Cytometry28.3 ± 8.9 pMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0850 289414532017Development of a DNA Aptamer for Screening Neisseria meningitidis Serogroup B by Cell SELEXK4N/AN/A5'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNANeisseria Meningitidis Serogroup B (ATCC 13090)Whole cellIdentity aptamers bind to and detect N. meningitidis in patients’ CSF samples.N/A6Flow Cytometry39.1 ± 8.6 pMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0851 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-5AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.Display a LOD of 10(4) with a linear range from 10(4) to 10(7) cfu/mL.12Flow Cytometry10.69 ± 0.89 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0852 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-2AGCAGCACAGAGGTCAGATG-GCCTGGCCAGGTGCCCCGATATAGCGACGCCTTGCCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0853 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-4AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCCCGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0854 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-12AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0855 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-1AGCAGCACAGAGGTCAGATG-CGCCCTACACCACTCTCGGAGCGCTGTACGGCATCCCTGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0856 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-15AGCAGCACAGAGGTCAGATG-GCCCGCACCACACACAGATGTCCATGTGTGCGCTGCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0857 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-11AGCAGCACAGAGGTCAGATG-GCCTGGCCAGGTGCCCCGATATAGCGACGCCTTGCCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0858 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-23AGCAGCACAGAGGTCAGATG-GCCTGGCCAGGTGCCCCGATATAGCGACGCCTTGCCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0859 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-14AGCAGCACAGAGGTCAGATG-GCCTGGCCAGGTGCGCCGATATAGCCTGCCCTTGCCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0860 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-18AGCAGCACAGAGGTCAGATG-GCCTGGCCACGTGCGCGGATATAGCCTGCCCTTGGCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0861 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-9AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCCCGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0862 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-25AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCCCGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0863 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-28AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCCCGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0864 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-7AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCGGGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0865 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-6AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0866 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-17AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0867 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-21AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0868 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-26AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0869 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-27AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0870 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-30AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0871 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-29AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCGGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0872 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-3AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCGGGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0873 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-13AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0874 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-16AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0875 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-22AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0876 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-24AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0877 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-19AGCAGCACAGAGGTCAGATG-GTCACACGGCCCGTCTGCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0878 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-20AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTCTGGCACGCCGGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0879 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-8AGCAGCACAGAGGTCAGATG-GCCCGCGCCCCTTCTGCCGTTGGTCGGAACCCCTGTGCGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0880 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-10AGCAGCACAGAGGTCAGATG-GCCCGCGCGGGTTCTGCCGTTGGTGGGAACCCCTGTGCGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0881 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB2pAGCAGCACAGAGGTCAGATG-CCGGGCAGCGGTCAATGCCGCACCTTCCATATGATCGGGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.The per cent gated fluorescence intensity above the background was 8.9% for BB2p.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0882 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10pAGCAGCACAGAGGTCAGATG-GGCCCCCTGCCTGCCAAAAAGGTGTTGCCAGGTTGGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.The per cent gated fluorescence intensity above the background was 11.2% for BB10p.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0883 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16pAGCAGCACAGAGGTCAGATG-CTCCCAGGCCGTTGGGGCGTTGCCTGCGTGCACCCGGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.The per cent gated fluorescence intensity above the background was 7.0% for BB16p.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0884 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB2CCGGGCAGCGGTCAATGCCGCACCTTCCATATGATCGGGG405'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.BB2 showed a lower binding affinity than the aptamer BB2p.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0885 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10GGCCCCCTGCCTGCCAAAAAGGTGTTGCCAGGTTGGCGGC405'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.Demonstrated higher binding affinity than the original full-length aptamer.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0886 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16CTCCCAGGCCGTTGGGGCGTTGCCTGCGTGCACCCGGGGC405'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.Demonstrated higher binding affinity than the original full-length aptamer.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0887 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-6fGGCCCCCTGCCTGCCAAAAAGGTGTTGCCAGGTT345'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0888 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-10fGGCCCCCTGCCTGCCAAAAAGGTGTTGCCA305'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0889 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-14fGGCCCCCTGCCTGCCAAAAAGGTGTT265'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0890 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-2rCCCCCTGCCTGCCAAAAAGGTGTTGCCAGGTTGGCGGC385'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0891 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-9rCCTGCCAAAAAGGTGTTGCCAGGTTGGCGGC315'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0892 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-13rCCAAAAAGGTGTTGCCAGGTTGGCGGC275'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0893 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16-11fCTCCCAGGCCGTTGGGGCGTTGCCTGCGT295'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.LOD of the assay was 1000 cfu/mL with a linear range from 10(3) to 10(7) cfu/mL.12Flow Cytometry18.66 ± 1.41 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0894 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16-13fCTCCCAGGCCGTTGGGGCGTTGCCTGC275'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0895 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16-15fCTCCCAGGCCGTTGGGGCGTTGCCT255'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0896 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16-8rCCGTTGGGGCGTTGCCTGCGTGCACCCGGGGC325'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0897 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-3ATACCAGCTTATTCAATTCCA-TGGTCCCTCGTGTTTATTATGTTGTCTGAACTGGCTG-AGATTGCACTTACTATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.Sandwich complex pair of SS-3 and SS-4 shows a linear dynamic range of 10(3)-10(6) cells/mL, and a limit of detection of 10(3) cells per mL for S. sonnei.8Surface Plasmon Resonance (SPR)39.32 ± 5.02 nMBiosensorWhole Cell-SELEX5'-Cyanine5 (Cy5) Labeled or 5′-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0898 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-4ATACCAGCTTATTCAATTCCA-CACATACCAAAAACACAGCACACTTCATCAATTTCACG-AGATTGCACTTACTATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.Sandwich complex pair of SS-3 and SS-4 shows a linear dynamic range of 10(3)-10(6) cells/mL, and a limit of detection of 10(3) cells per mL for S. sonnei.8Surface Plasmon Resonance (SPR)15.89 ± 1.77 nMBiosensorWhole Cell-SELEX5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0899 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-1ATACCAGCTTATTCAATTCCA-GGGCATGTGGACCTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0900 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-2ATACCAGCTTATTCAATTCCA-CCCCATGTCTGTTTCTTTTAACAGGTAATCCGCCTTATGC-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0901 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-5ATACCAGCTTATTCAATTCCA-CAGGACAAAAATTCGGGAAGGCGGCCTTCCACTCTTTCTG-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0902 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-6ATACCAGCTTATTCAATTCCA-ATACAGTGAAGGCCCAGGAGGCAAATTCTAGGACGAAAGC-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0903 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-7ATACCAGCTTATTCAATTCCA-CACCAAGTCGCCTCCTCTGCCCCCATGTAAATCGTTACAT-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0904 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-8ATACCAGCTTATTCAATTCCA-GAACAATTTCACGCACACCGCGTAGATACCACCTCGTGCA-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0905 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-9ATACCAGCTTATTCAATTCCA-CGACGAACTACACCACAGGCCCTTAACACATCTACTTGTA-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0906 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-10ATACCAGCTTATTCAATTCCA-CATGCTAACTGCCATCACCACTATTTATGATTCCATCCAT-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0907 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-11ATACCAGCTTATTCAATTCCA-CGCAACACAGAAACACCCGTACACAAACAGTTTCAGCCCG-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0908 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-12ATACCAGCTTATTCAATTCCA-TGCGTAGTTCTCTTAACAACCATTCAGCGTATTTTCGTGG-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0909 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-13ATACCAGCTTATTCAATTCCA-CAGGCACGAGTTCATCACACACCATACACAGTTCGTTACT-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0910 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-14ATACCAGCTTATTCAATTCCA-CCACACCACACACTACACGCATAAAACCACGAAACTACAC-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0911 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-15ATACCAGCTTATTCAATTCCA-GGGTATGTGGACTTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0912 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-16ATACCAGCTTATTCAATTCCA-CATACAGTGGCCAGATTTACCAACACACCTTTCCATACGC-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0913 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-17ATACCAGCTTATTCAATTCCA-CCACTCACATATTAACAACACCACATGAATATATTTCG-AGATTGCACTTACTATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0914 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-18ATACCAGCTTATTCAATTCCA-ACAGGGCATGTCTTCATCAACGCTTACCACACACCGCTCG-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0915 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-19ATACCAGCTTATTCAATTCCA-CACTCATGCGCACCGCGTCGCACTTAATCAGTGTTGTGC-AGATTGCACTTACTATCT785'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0916 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-20ATACCAGCTTATTCAATTCCA-GGGCATGTGGACTTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0917 285135592017Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor PlatformSS-21ATACCAGCTTATTCAATTCCA-CAGACACACCAACGCACGGGCACACGCTACAAATGTAAGT-AGATTGCACTTACTATCT795'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAShigella Sonnei (KCTC 2518)Whole cellIdentify and develop an aptamer-based fluorescent biosensor assay to detect and discriminate S. sonnei cells.N/A8Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0920 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 1GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATC555'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.The detection time for M. tuberculosis was 70 min, and the detection limit was 100 cfu/mL.14Fluorescence Spectroscopy37 ± 4 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0921 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 2GGGAGCTCAGAATAAACGCTCAA-GGCACACAGGACTATACAGTGTTGCAGTGTTGCTG-TTCGACATGAGGCCCGGATC555'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy97 ± 4 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0922 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 3GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATC555'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy101 ± 5 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0923 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 4GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATC555'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy98 ± 7 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0924 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 5GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTACCCGATATGTGTCCGAGTGGTG-TTCGACATGAGGCCCGGATC555'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy96 ± 8 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0925 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 6GGGAGCTCAGAATAAACGCTCAA-GGCACGATGTGGCTACATCGATCGCGGTACTGGTG-TTCGACATGAGGCCCGGATC555'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy103 ± 6 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0926 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 7GGGAGCTCAGAATAAACGCTCAA-GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy107 ± 9 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0927 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 8GGGAGCTCAGAATAAACGCTCAA-GGCAGCAGCAATGTAACACTGTGTGTATGTGTTGG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy102 ± 8 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0928 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer 9GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy108 ± 4 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0929 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer10GGGAGCTCAGAATAAACGCTCAA-CCCTGCGGGGCTGCCCGATATGTGTCCAGGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy110 ± 3 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0930 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer11GGGAGCTCAGAATAAACGCTCAA-GGCACGATGTGGCTACATCGATCGCGGTACTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy103 ± 4 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0931 286891122017Selection of a new Mycobacterium tuberculosis H37Rv aptamer and its application in the construction of a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensorAptamer12GGGAGCTCAGAATAAACGCTCAA-GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify an aptamer against H37Rv and develop a SWCNT/aptamer/Au-IDE MSPQC H37Rv sensor to distinguish it from M. smegmatis and BCG strain.N/A14Fluorescence Spectroscopy110 ± 7 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0933 284546522017Selection, characterization, and application of DNA aptamers for detection of Mycobacterium tuberculosis secreted protein MPT64Aptamer 17TCACTTCAAATGTGCGCTTC-GTCAACAGTCCAGTTGGGAGGTCGCTTATAGCTGCTTGTC-CGTCAAAACAGGGGGTAGAA805'-TCACTTCAAATGTGCGCTTC-N40-CGTCAAAACAGGGGGTAGAA-3'ssDNAMycobacterium Tuberculosis (H37Rv)MPT64 protein (Rv1980c)Identify DNA aptamers against MPT64 protein.The ELONA assay had a sensitivity and specificity of 91.3% and 90% on sputum samples.10Surface Plasmon Resonance (SPR)8.92 nMDetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_0935 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.1AGCGC(U^y)CGCGCGGCG(U^y)GC-ACAACAAA(U^y)G(U^y)GACCA(U^y)GCGA(U^y)(U^y)CCCCA(U^y)A(U^y)CCAGGCACA-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0936 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.2AGCGC(U^y)CGCGCGGCG(U^y)GC-GA(U^y)GCG(U^y)G(U^y)GG(U^y)G(U^y)GGAG(U^y)(U^y)G(U^y)G(U^y)GAG(U^y)GG(U^y)C(U^y)GCG(U^y)(U^y)G(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0937 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.5BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)(U^y)CA(U^y)GCCC(U^y)G(U^y)G(U^y)C(U^y)(U^y)GC(U^y)C(U^y)(U^y)G(U^y)GAG(U^y)(U^y)G(U^y)(U^y)G(U^y)G(U^y)CA-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0938 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.8AGCGC(U^y)CGCGCGGCG(U^y)GC-G(U^y)G(U^y)G(U^y)A(U^y)GCG(U^y)(U^y)CA(U^y)G(U^y)GG(U^y)GAGG(U^y)C(U^y)(U^y)GCG(U^y)C(U^y)G(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0939 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.10AGCGC(U^y)CGCGCGGCG(U^y)GC-GAG(U^y)G(U^y)G(U^y)G(U^y)GGG(U^y)CCGAG(U^y)GGG(U^y)GG(U^y)CAGGG(U^y)(U^y)(U^y)G(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.Showed the highest % binding to the DH5α cells, with per cent bound ranging from 2.9 to 21%.12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0940 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.14BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)C(U^y)G(U^y)G(U^y)GCGG(U^y)G(U^y)(U^y)A(U^y)GCGGG(U^y)(U^y)GGG(U^y)(U^y)G(U^y)(U^y)(U^y)G(U^y)G(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.Showed the highest % binding to the DH5α cells, with per cent bound ranging from 2.9 to 21%.12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0941 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.16BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)(U^y)(U^y)GCG(U^y)CCGGG(U^y)(U^y)(U^y)A(U^y)GCGGG(U^y)(U^y)G(U^y)C(U^y)CG(U^y)G(U^y)C(U^y)G(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0942 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.17BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)C(U^y)(U^y)G(U^y)(U^y)GGG(U^y)CGACGG(U^y)C(U^y)G(U^y)(U^y)CA(U^y)G(U^y)GGG(U^y)G(U^y)(U^y)G(U^y)CCC-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0943 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.18BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)(U^y)G(U^y)G(U^y)(U^y)CACG(U^y)CA(U^y)(U^y)(U^y)CGCAC(U^y)CC(U^y)C(U^y)CAGC(U^y)ACG(U^y)(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.Showed the highest % binding to the DH5α cells, with per cent bound ranging from 2.9 to 21%.12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0944 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.24AGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)G(U^y)G(U^y)(U^y)(U^y)G(U^y)GG(U^y)ACGAGCG(U^y)G(U^y)G(U^y)GGA(U^y)GG(U^y)CCG(U^y)G(U^y)CA-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0945 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.28AGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)CC(U^y)CGCG(U^y)(U^y)(U^y)GGA(U^y)(U^y)CA(U^y)G(U^y)(U^y)GG(U^y)(U^y)(U^y)G(U^y)CGG(U^y)G(U^y)A(U^y)(U^y)G(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.Showed the highest % binding to the DH5α cells, with per cent bound ranging from 2.9 to 21%.12Saturation Binding Assay27.4 ± 18.7 nMDetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP) and Radiolabelled ([γ-32P]-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0946 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.30AGCGC(U^y)CGCGCGGCG(U^y)GC-ACAGAAAG(U^y)G(U^y)GGCCA(U^y)G(U^y)G(U^y)(U^y)G(U^y)G(U^y)CCC(U^y)GGCAGG(U^y)(U^y)AG(U^y)(U^y)(U^y)A-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC815'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0947 285511172017Electrochemical determination of M. tuberculosis antigen based on Poly(3,4-ethylenedioxythiophene) and functionalized carbon nanotubes hybrid platformMPT64 aptamerGTACAAACGACGGCCAGT-CCTTGGGATGATTCAAGCAAAGCCTCACGCCTACGGCTAA-GTCATAGCTGTCTCTCCTG775'-GTACAAACGACGGCCAGT-N40-GTCATAGCTGTCTCTCCTG-3'ssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDeveloped an electrochemical aptasensor based on MWCNT-doped PEDOT nano-composite for the detection of Mycobacterium tuberculosis.Exhibits a LOD of 0.5 fg/mL within 15 min for the detection of M. tb antigenic protein with recovery in between (88–95)%.N/AN/AN/ABiosensorSELEX5'-BiotinylatedN/AThe stability of the aptasensor is 27 days at 4°C, and the reusability is 7 times after repeated regeneration with 50 mM NaOH.N/AN/AN/A
ABdb_0979 https://doi.org/10.1007/s00604-017-2174-72017Aptamer based voltammetric biosensor for the detection of Mycobacterium tuberculosis antigen MPT64Aptamer 3GTACAAACGACGGCCAGT-CCTTGGGATGATTCAAGCAAAGCCTCACGCCTACGGCTAA-GTCATAGCTGTCTCTCCTG775'-GTACAAACGACGGCCAGT-N40-GTCATAGCTGTCTCTCCTG-3'ssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDevelop an aptaelectrode based on graphene modified iron-oxide chitosan hybrid (CHIT-IO-GR) nanocomposite film deposited on fluorine tin oxide (FTO) for the detection of the M. tb. specific antigen MPT64.Exhibited a limit of detection (LOD) of 0.9 fg/mL within 20 min and a linear range from 1.0 × 10(5) fg/mL to 1.0 fg/mL.10N/AN/ABiosensorSELEX5'-BiotinylatedN/AThe biosensor retained about 80% of its initial activity after 10 uses and good stability of 17 day.Best CandidateN/AN/A
ABdb_0982 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-01CATATCCGCGTCGCTGCGCTCAGACCCACCACTACGCACC405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0983 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-02CATATCCGTGTCGCTGCGCTCAGACCCACCACCACGCACC405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0984 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-03CATATCCGCGTCGCTGCGCTCAGACCCACCACCACGCACC405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.STC-03 showed better affinity to E. aerogenes, K. pneumoniae, C. freundii, and B. subtilis.18Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)27.2 nM (for E. coli), 11.9 nM (for E. aerogenes), 9.22 nM (for K. pneumoniae), 15.9 nM (for C. freundii), 9.97 nM (for B. subtilis), and 16.4 nM (for S. epidermidis)DetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0985 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-04ATATCCGCGTCGCTGCGCTCAGACCCACCACCACGCACC395'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0986 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-05CATATCCGCGTCGCTGCGCTCAGACCCACCACCCGCCC385'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0987 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-06GGGCGGGGGTGCTGGGGGAATGGAGTGCTGCGTGCTGCGG405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0988 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-07GGGCGGGGGGTGCTGGGGGAATGGAGTGCTGCGTGCTGCG405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0989 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-08GGGCGGGGGTGCTGGGAGAATGGAGTGCTGCGTGCTGCAG405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0990 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-09GGGCAGGTGTGCTGGGGGAATGGAGTGCTGCGTGCTGCGG405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0991 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-10AGACCGCAGGTGCACCGGGCGACGTCTCTGGGTGTGGTGA405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0992 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-11AGACCGCAGGTGCACTGGGCGACGTCTCTGGGTGTGGTGT405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0993 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-12GGACCGCAGGTGCACTGGGCGACGTCTCTGGGTGTGGTGT405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.STC-12 showed better affinity to E. coli and S. epidermidis.18Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)38.5 nM (for E. coli), 33.7 nM (for E. aerogenes), 16.3 nM (for K. pneumoniae), 25.6 nM (for C. freundii), 18.9 nM (for B. subtilis), and 13.7 nM (for S. epidermidis)DetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0994 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-13GGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0995 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-14CGGGGTGGGACCAGTCTTGCGCGGGTGAC295'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0996 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-15GGACTGGAGTCTAGACCGGGTAGCTGTGGT305'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.N/A18N/AN/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1003 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH63GTCTTGACTAGTTACGCC-GGGAACAATATGTTCAAGGGCTCTTTAAAGTTTTAGTTCGTTTG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.LOD was 32 ng for H63.8Isothermal Titration Calorimetry (ITC)3.71 x 10-7 ± 2.12 x 10-11 MDiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AIndian Patent application no. 201611001550
ABdb_1004 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH63 SL-2 M6AGGGCTTTTTTTTTTTTTAGTTCGTTTG285'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.Exhibited 2.5-fold higher binding with respect to the parent H63, and H63 SL-2 M6 has an LOD of 16 ng.8Isothermal Titration Calorimetry (ITC)9 x 10-8 ± 2 x 10-14 MDiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AIndian Patent application no. 201611001550
ABdb_1005 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH33GTCTTGACTAGTTACGCC-ACACTAGAAGGTATTTACTATTCTGTGTTAACATGTACTAGCCA-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1006 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH66GTCTTGACTAGTTACGCC-AAGTTTTAATACAACTCACATTAGGGAAAGCTTGCATCCAGGCC-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1007 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH70GTCTTGACTAGTTACGCC-CAGGACAGGTTAAAATTTTTCTGGATCCCTTTTCATTCTCCATGG-TCATTCAGTTGGCGCCTC815'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1008 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH3GTCTTGACTAGTTACGCC-CATACACGGTTACACGTACTGGATAATGTTGATTATGCTTCTGC-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1009 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH18GTCTTGACTAGTTACGCC-TAGAACCAAACCTTGTGTAGAAGGGCTTCTCCCGTGTGCTTTGA-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1010 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH47GTCTTGACTAGTTACGCC-GGATGAACTGTTCCGCTGTGTATCACGCCAAAAGTCCTTATTTG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1011 302059662018Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitisH48GTCTTGACTAGTTACGCC-CATGAAACCATCTGGTCCGCAATGGGCGCTTGGAATATTCGTCA-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenIdentify an aptamer that binds to HspX and develop an assay to diagnose TBM in CSF samples.N/A8Isothermal Titration Calorimetry (ITC)N/ADiagnosticSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1012 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB10TAGCTCACTCATTAGGCAC-TCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.A dual-labelled sandwich detection system was developed using biotinylated RAB10 and FITC-labelled RAB35, with a limit of detection (LOD) of 10^2 CFU/mL of S. aureus, and showed 2.7-fold more signal than other bacteria.10Flow Cytometry46 ± 24 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1013 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB20TAGCTCACTCATTAGGCAC-GCGTTACGTTAGTGGCCGCCTATGAGGACAGGCGGTTGTA-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry128 ± 45 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1014 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB28TAGCTCACTCATTAGGCAC-TGGACGTCGTGGCGGAGGTTTTATAAAACGGCGCCACTGT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry49 ± 39 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1015 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB35TAGCTCACTCATTAGGCAC-GGGGGGTTGTGCCATTTAAGATGACCGGTTGCCGCGATTT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.A dual-labelled sandwich detection system was developed using biotinylated RAB10 and FITC-labelled RAB35, with a limit of detection (LOD) of 10^2 CFU/mL of S. aureus, and showed 2.7-fold more signal than other bacteria.10Flow Cytometry34 ± 5 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1016 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB1TAGCTCACTCATTAGGCAC-CGGGTGGGCTCCAATATGAATCGCTTGCCCTGACGCTATCT-GCATAGTTAAGCCAGCC775'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry56 ± 87 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1017 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB3TAGCTCACTCATTAGGCAC-CGTAGTCTAGTGTCGATTAGTTTCCTTGAGACCTTGTGCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry37 ± 112 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1018 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB5TAGCTCACTCATTAGGCAC-CGTAGTCTAGTGTCGATTAGTTTCCTTGCTATTGCAGACCTTGTGCT-GCATAGTTAAGCCAGCC835'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry58 ± 14 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1019 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB9TAGCTCACTCATTAGGCAC-TCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1020 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB21TAGCTCACTCATTAGGCAC-GGGGGGTTGTGCCATTTAAGATGACCGGTTGCCAAGATG-GCATAGTTAAGCCAGCC755'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1021 290985172018Influence of aptamer-targeted antibiofilm agents for treatment of Pseudomonas aeruginosa biofilmsPA-ap1 (F23)CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellAptamer-ciprofloxacin-SWNTs complex for antibiofilm activity.90% inhibitory efficiency of complex aptamer-ciprofloxacin-SWNTs on biofilm formation.16Flow Cytometry17.27 ± 5.00 nMTargeted Delivery/TherapeuticsWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1022 295809442018Inhibition of anthrax lethal factor by ssDNA aptamersML12 (30mer)CGAGGGAGACGCGAACCTTCTCGCCTTGGG305'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNABacillus Anthracis (BA)Lethal factor (LF)Identify aptamers against LF and interfere with the interaction with its substrate MEK1 by blocking the active site of LF to prevent protease activity.Effective inhibitor of LF with an IC₅₀ of 15 ± 1.5 µM and ~85% cell viability.8Fluorescence Spectroscopy11.0 ± 2.7 nMTherapeuticsSELEX3'-Cyanine3 (Cy3) LabeledIn RAW 264.7 cells, the cell viability remained unchanged from 0.08 to 10 µM of ML12, indicating that it appears to be non-toxic.N/ABest CandidateN/AN/A
ABdb_1023 295809442018Inhibition of anthrax lethal factor by ssDNA aptamersML6GGACCAGCCGCCGCGCCTTGACCGGGGGTA305'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNABacillus Anthracis (BA)Lethal factor (LF)Identify aptamers against LF and interfere with the interaction with its substrate MEK1 by blocking the active site of LF to prevent protease activity.N/A8Fluorescence SpectroscopyN/ATherapeuticsSELEX3'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1024 295809442018Inhibition of anthrax lethal factor by ssDNA aptamersML7GGAGAGAGGGAGACGCGCAACCTCGACCCGT315'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNABacillus Anthracis (BA)Lethal factor (LF)Identify aptamers against LF and interfere with the interaction with its substrate MEK1 by blocking the active site of LF to prevent protease activity.N/A8Fluorescence SpectroscopyN/ATherapeuticsSELEX3'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1025 295809442018Inhibition of anthrax lethal factor by ssDNA aptamersML12 (60mer)GCGCGGATCCCGCGC-CGAGGGAGACGCGAACCTTCTCGCCTTGGG-CGCGCGAAGCTTGCG605'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNABacillus Anthracis (BA)Lethal factor (LF)Identify aptamers against LF and interfere with the interaction with its substrate MEK1 by blocking the active site of LF to prevent protease activity.N/A8Fluorescence Spectroscopy17.5 ± 4.8 nMTherapeuticsSELEX3'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1026 299640282018Selection of DNA aptamers to Streptococcus pneumonia and fabrication of graphene oxide based fluorescent assayLyd-3TGACGAGCCCAAGTTACCT-GCCCCCGAACCATACCACACGATGCCCCGTACCCCAGCCACC-AGAATCTCCGCTGCCTACA805'-TGACGAGCCCAAGTTACCT-N42-AGAATCTCCGCTGCCTACA-3' (Library 1) and 5'-AAGGGCTGGCTGGGATGGA-N42-TCACTTCACGGACCCCACT-3' (Library 2)ssDNAStreptococcus PneumoniaeWhole cellIdentify aptamers that bind to and develop a graphene oxide/aptamer-based label-free fluorescent assay to detect Streptococcus pneumoniae and inhibit biofilm formation.Show a detection limit of 15 cfu mL-1. Biofilm formation was reduced from 100% to 35.8% at a Lyd-3 concentration of 1 μM compared with the no-aptamer control.20Fluorescence Spectroscopy661.8 ± 111.3 nMDiagnostic/TherapeuticsWhole Cell-SELEXFAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1027 299640282018Selection of DNA aptamers to Streptococcus pneumonia and fabrication of graphene oxide based fluorescent assayLyd-1AAGGGCTGGCTGGGATGGA-CCCTCCCGAAACGAGCTGTCTCTTAACGGAAGCTAATCTGCC-TCACTCCACGGACCCCACT805'-TGACGAGCCCAAGTTACCT-N42-AGAATCTCCGCTGCCTACA-3' (Library 1) and 5'-AAGGGCTGGCTGGGATGGA-N42-TCACTTCACGGACCCCACT-3' (Library 2)ssDNAStreptococcus PneumoniaeWhole cellIdentify aptamers that bind to and develop a graphene oxide/aptamer-based label-free fluorescent assay to detect Streptococcus pneumoniae and inhibit biofilm formation.At 100 nM of Lyd-1, biofilm formation decreased to 90.8%.20Fluorescence Spectroscopy844.7 ± 123.6 nMDiagnostic/TherapeuticsWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_1028 299640282018Selection of DNA aptamers to Streptococcus pneumonia and fabrication of graphene oxide based fluorescent assayLyd-2TGACGAGCCCAAGTTACCT-CACCCGCCTGGCAAAAAACACCACGACATTTTTCTCACCCCC-AGAATCTCCGCTGCCTACA805'-TGACGAGCCCAAGTTACCT-N42-AGAATCTCCGCTGCCTACA-3' (Library 1) and 5'-AAGGGCTGGCTGGGATGGA-N42-TCACTTCACGGACCCCACT-3' (Library 2)ssDNAStreptococcus PneumoniaeWhole cellIdentify aptamers that bind to and develop a graphene oxide/aptamer-based label-free fluorescent assay to detect Streptococcus pneumoniae and inhibit biofilm formation.At 100 nM of Lyd-2, biofilm formation decreased to 92.6%.20Fluorescence Spectroscopy1984.8 ± 347.5 nMDiagnostic/TherapeuticsWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_1029 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-12AGCAGCACAGAGGTCAGATG-GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.Limit of detection of 70 cfu/mL with a range of 70–7100 cfu/mL.12Flow Cytometry44 ± 5 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1030 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-2AGCAGCACAGAGGTCAGATG-GTTCGGGGTCGGGGTGAGTGGGGCCTAGGAGTGGGGGCGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1031 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-8AGCAGCACAGAGGTCAGATG-ATGGGGGGCGGGGAGGTGGGTACAGGGTCGGGGATGGCAG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1032 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-10AGCAGCACAGAGGTCAGATG-CGGGCGGGGCGTGGGGTGTTGGAGTGGAGGGCGGGGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow Cytometry54  ±  8 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1033 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-15AGCAGCACAGAGGTCAGATG-CAGGGTGCGGGAGGGCCAAAGGGGGGAGGGCCCGGGGGGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1034 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-21AGCAGCACAGAGGTCAGATG-GGGGGAGGCGCCGGGCAGGGGGCTCGGGGGAGGCGGGCGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1035 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-26AGCAGCACAGAGGTCAGATG-TTCAGGGCGGGGTAAACGGGAGGTGGGGGGGGCTTGGGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow Cytometry130  ±  24 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1036 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-28AGCAGCACAGAGGTCAGATG-TAATACTACCAACTTCTTTGCCTGGCGTAAGTAACAGTCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow Cytometry132 ± 18 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1037 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-32AGCAGCACAGAGGTCAGATG-TACACAATGCTTCGATAATTGACGCTACTTCATTTTATTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1038 3056304420182'-Fluoro-Pyrimidine-Modified RNA Aptamers Specific for Lipopolysaccharide Binding Protein (LBP)A011CCAAGCUUGCAUGCCUGCAG-GGACUGUGGAUUGUUCUGGGUGCCGCUUCUCCGAAUAUCGGACUCUCGAACUCCUGGGGA-GGUACCGAGCUCGAAUUCCC1005'-CCAAGCTTGCATGCCTGCAG-N60-GGTACCGAGCTCGAATTCCC-3'ssRNAGram-negative BacteriaMurine Lipopolysaccharide Binding Protein (mLBP)Identify aptamers that bind to and inhibit the LPS-LBP interaction or block of LBP-mediated transfer of LPS monomers to CD14.N/A8Nitrocellulose Filter Binding Assay267 ± 48 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/ABest CandidateN/AN/A
ABdb_1039 3056304420182'-Fluoro-Pyrimidine-Modified RNA Aptamers Specific for Lipopolysaccharide Binding Protein (LBP)A036CCAAGCUUGCAUGCCUGCAG-GAUAUACGGUGCCGCUUCUCUAACCGGUGGUCAUGCGGUUGGACGCUCGAACAGACUA-GGUACCGAGCUCGAAUUCCC985'-CCAAGCTTGCATGCCTGCAG-N60-GGTACCGAGCTCGAATTCCC-3'ssRNAGram-negative BacteriaMurine Lipopolysaccharide Binding Protein (mLBP)Identify aptamers that bind to and inhibit the LPS-LBP interaction or block of LBP-mediated transfer of LPS monomers to CD14.N/A8Nitrocellulose Filter Binding AssayN/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1040 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT916CATCCGTCACACCTGCTC-GGCAAAAAGGATTGCCCAGGTCTGCTGTCTAGCCGGATTC-GGTGTTGGCTCCCGTATC765'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.Detection limits of 2.1 ng/ml in buffer and 2.4 ng/ml in tap water, with a linear range from about 1 ng/ml to 1000 ng/ml.12Bead-based Affinity Chromatography48.5 ± 0.5  nMBiosensorSELEXAmidation (NH₂-(CH2)6) or Biotinylated and AlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_1041 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT910CATCCGTCACACCTGCTC-GTACACAGGAGCCAAACCCAACCAGACCAACCTGAAGCC-GGTGTTGGCTCCCGTATC755'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography54.6 ± 6.7 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1042 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT928CATCCGTCACACCTGCTC-CACCCGTCACACTTATCCGTCAACTGGGCTCCCACTGGG-GGTGTTGGCTCCCGTATC755'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography23.6 ± 1.2 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1043 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT931CATCCGTCACACCTGCTC-CCAGCGTGCGTCGAGGGGGACCCCTGTCAGCCCCCTCGCG-GGTGTTGGCTCCCGTATC765'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography50.2 ± 13.2 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1044 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT938CATCCGTCACACCTGCTC-CACCCGTCCACTCATCCGTCACACCTGTTCTCCCCATG-GGTGTTGGCTCCCGTATC745'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography23.2 ± 0.4 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1045 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT939CATCCGTCACACCTGCTC-CACCCGTCACACTTATCCGTCACACTGGGCCCCACTGGG-GGTGTTGGCTCCCGTATC755'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography41.6 ± 4.0 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1046 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT943CATCCGTCACACCTGCTC-CACCCGTCCACTCATCCGACACACCTGCTCTCCCCACTG-GGTGTTGGCTCCCGTATC755'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography26.8 ± 0.5 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1047 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT946CATCCGTCACACCTGCTC-CCCACAACCGGTCCGGTGTTCGGTCCCCGTATCACGCCCC-GGTGTTGGCTCCCGTATC765'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography30.4 ± 1.5 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1048 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-2TCAGCACGT-ATAAGCATGAATTGACCAACCTAAACTTATTCATTTTCCAGCACCTCTAATATTACTGGC-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.75% binding affinity to V. parahaemolyticus than to other foodborne bacteria (less than 18%).8Flow Cytometry10.3 ± 4.5 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1049 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-1TCAGCACGT-AGCCCTATTTGTTTTCTCTTGTCTCTGTACTGCTGATGTTGGTCATTCTCTTTTCTCGGT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry19.2 ±  2.8  nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1050 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-3TCAGCACGT-TATCTAGTCAGATATCCAGACAGTCGCGGCTGGAAGCTTCTGTTAAGAATTTGAGATACT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry25.2  ±  3.1 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1051 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-4TCAGCACGT-GCGGGCATTATTGCACTCCACGGCGAGTAGTGCTCACAGAGTCATTTACACCGGTCGTAT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry17.2  ±  5.1 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1052 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H7Apt-1TGAGCCCAAGCCCTGGTATG-TTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry18.97 ± 1.73 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1053 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H8Apt-2TGAGCCCAAGCCCTGGTATG-TGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry20.73 ± 2.29 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1054 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H9Apt-3TGAGCCCAAGCCCTGGTATG-TAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry16.44 ± 2.16 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1055 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H10Apt-4TGAGCCCAAGCCCTGGTATG-AGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry15.13 ± 0.88 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1056 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H11Apt-5TGAGCCCAAGCCCTGGTATG-CGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Lipopolysaccharide (LPS)Identify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.Apt-5 shows a strong affinity for all stages of bacterial growth (adjustment, log, and stationary phases).14Flow Cytometry9.04 ± 2.08 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1057 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H12Apt-6TGAGCCCAAGCCCTGGTATG-GTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry36.67 ± 4.55 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1058 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H13Apt-7TGAGCCCAAGCCCTGGTATG-TGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry17.11 ± 0.31 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1059 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H14Apt-8TGAGCCCAAGCCCTGGTATG-TCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCA-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry24.68 ± 1.95 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1060 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H15Apt-9TGAGCCCAAGCCCTGGTATG-TTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry20.99 ± 0.37 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1067 292421482018Whole-bacterium SELEX of DNA aptamers for rapid detection of E.coli O157:H7 using a QCM sensorS1CAGTCCAGGACAGATTCGCGAG-TGGTCGTGGTGAGGTGCGTGTATGGGTGGTGGATGAGTGTGTGGC-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellIdentify aptamers specifically bound to Escherichia coli O157:H7 and develop a QCM-based aptasensor for its detection.The limit of detection (LOD) was 1.46 × 10^3 CFU/mL, with a total detection time of 50 minutes.19Quartz Crystal Microbalance (QCM)10.30 nMBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1068 292421482018Whole-bacterium SELEX of DNA aptamers for rapid detection of E.coli O157:H7 using a QCM sensorS2CAGTCCAGGACAGATTCGCGAG-GCGGGAATAGGATGCGGCTGGAAGGAGAGGTGTTGGTGGGTGGTG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellIdentify aptamers specifically bound to Escherichia coli O157:H7 and develop a QCM-based aptasensor for its detection.N/A19Quartz Crystal Microbalance (QCM)N/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1069 292421482018Whole-bacterium SELEX of DNA aptamers for rapid detection of E.coli O157:H7 using a QCM sensorS3CAGTCCAGGACAGATTCGCGAG-GTGCGGTGACGTGAGGGGGAGAGGCGTTGGTGTAGGCTGTTGGTG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellIdentify aptamers specifically bound to Escherichia coli O157:H7 and develop a QCM-based aptasensor for its detection.N/A19Quartz Crystal Microbalance (QCM)N/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1071 297661802018A nitrocellulose membrane-based integrated microfluidic system for bacterial detection utilizing magnetic-composite membrane microdevices and bacteria-specific aptamersAptamerACAGCACCACAGACCACATATCACATGCTGTCGCCTTGCGATATCAATTCCAGTGATGTTTGTCTTCCTGCC72N/AssDNAAcinetobacter BaumanniiWhole cellA nitrocellulose membrane-based integrated microfluidic system for detecting bacteria.Display a limit of detection as low as 450 CFU/reaction for AB within 40 minutes and a linear range from 4.5×10(4) to 4.5 CFU/reaction.N/AN/AN/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1073 292608612018Whole-Cell Pseudomonas aeruginosa Localized Surface Plasmon Resonance AptasensorBt-aptamerATACCAGCTTATTCAATTCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTGAGATAGTAAGTGCAATCT96N/AssDNAPseudomonas Aeruginosa PAO1Whole cellA localized surface plasmon resonance (LSPR) based sensing platform was developed to detect the whole-cell Pseudomonas aeruginosa strain PAO1.Achieve a limit of detection (LOD) of 10 cfu/mL with a linear range of 10(0)–10(3) cfu/mL.N/AN/A17.27 ± 5.00 nMBiosensorWhole Cell-SELEX5′-Biotinylated Polyethene Glycol (Bt-PEG) thiol/PEG thiol (1:3) or 5'-6FAM-aptamer-Biotin-3'N/AStable in ambient conditions for ≥2 months.N/AN/AN/A
ABdb_1075 355476762018Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide systemPA1TGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGACGTGACCGCTGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGAC845'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify and develop an aptamer-based fluorescence assay (aptamer-CDs/GO system) for culture-independent detection of P. aeruginosa.Achieved a wide detection range from 10(1) to 10(7) CFU/mL with a detection limit as low as 9 CFU/mL.12N/A15.16 ± 3.62 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)N/AN/AN/ABest CandidateN/AN/A
ABdb_1076 355476762018Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide systemPA2TGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGACGTGACCGTTGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGAC845'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify and develop an aptamer-based fluorescence assay (aptamer-CDs/GO system) for culture-independent detection of P. aeruginosa.N/A12N/A14.11 ± 2.59 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)N/AN/AN/AN/AN/AN/A
ABdb_1077 355476762018Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide systemPA3TGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGACACGACGCATGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGAC845'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify and develop an aptamer-based fluorescence assay (aptamer-CDs/GO system) for culture-independent detection of P. aeruginosa.N/A12N/A32.59 ± 8.89 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)N/AN/AN/AN/AN/AN/A
ABdb_1078 355476762018Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide systemPA4TGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGACGCACGTTGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGAC825'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify and develop an aptamer-based fluorescence assay (aptamer-CDs/GO system) for culture-independent detection of P. aeruginosa.N/A12N/A16.39 ± 8.14 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)N/AN/AN/AN/AN/AN/A
ABdb_1079 355476762018Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide systemPA5TGGACCTTGCGATTGACAGCTCGTGGCACGTGCCGCTCGCCTTGGACCTTGCGATTGACAGCAGACATGAGTCTCAGGAC805'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify and develop an aptamer-based fluorescence assay (aptamer-CDs/GO system) for culture-independent detection of P. aeruginosa.N/A12N/A30.69 ± 7.15 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)N/AN/AN/AN/AN/AN/A
ABdb_1085 302454722018G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host CellsHupB-1GTCTTGACTAGTTACGCC-TAGGAAGGGAAGGAAGATAGAGAGTAGGTCGTCGGAGGCAGGGG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N3-41X-TCATTCAGTTGGCGCCTC-3' (*N = 3nt sequence tag and **X = 41nt random region)ssDNAMycobacterium Tuberculosis (M.Tb.)Histone-like protein HupB (Rv2986c)Identify aptamers that recognise and inhibit the DNA-binding activity of HupB, and significantly block Mtb entry into THP-1 monocytic cells.Displayed the highest binding to HupB (≥5 fold relative to RDL).8Isothermal Titration Calorimetry (ITC)N/ATherapeuticsSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/APatent application no. 20171100124
ABdb_1086 302454722018G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host CellsHupB-2GTCTTGACTAGTTACGCC-TAGTAAAGGGGGTAAGAATCAATTGCGAGGTGGAGTGGGAGTGG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N3-41X-TCATTCAGTTGGCGCCTC-3' (*N = 3nt sequence tag and **X = 41nt random region)ssDNAMycobacterium Tuberculosis (M.Tb.)Histone-like protein HupB (Rv2986c)Identify aptamers that recognise and inhibit the DNA-binding activity of HupB, and significantly block Mtb entry into THP-1 monocytic cells.Displayed the highest binding to HupB (≥5 fold relative to RDL).8Isothermal Titration Calorimetry (ITC)N/ATherapeuticsSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/APatent application no. 20171100124
ABdb_1087 302454722018G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host CellsHupB-4GTCTTGACTAGTTACGCC-TAGGTGATTGCTGGAGTGGGTAGCAGATGGGGGGGGGGTTCTTGG-TCATTCAGTTGGCGCCTC815'-GTCTTGACTAGTTACGCC-N3-41X-TCATTCAGTTGGCGCCTC-3' (*N = 3nt sequence tag and **X = 41nt random region)ssDNAMycobacterium Tuberculosis (M.Tb.)Histone-like protein HupB (Rv2986c)Identify aptamers that recognise and inhibit the DNA-binding activity of HupB, and significantly block Mtb entry into THP-1 monocytic cells.Displayed the highest binding to HupB (≥5 fold relative to RDL).8Isothermal Titration Calorimetry (ITC)N/ATherapeuticsSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/APatent application no. 20171100124
ABdb_1088 302454722018G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host CellsHupB-13GTCTTGACTAGTTACGCC-TTTGGGAAGGGGGGGGAGGAAGTTCGATGGTGTTGTGGACCGGG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N3-41X-TCATTCAGTTGGCGCCTC-3' (*N = 3nt sequence tag and **X = 41nt random region)ssDNAMycobacterium Tuberculosis (M.Tb.)Histone-like protein HupB (Rv2986c)Identify aptamers that recognise and inhibit the DNA-binding activity of HupB, and significantly block Mtb entry into THP-1 monocytic cells.Displayed the highest binding to HupB (≥5 fold relative to RDL).8Isothermal Titration Calorimetry (ITC)N/ATherapeuticsSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/APatent application no. 20171100124
ABdb_1089 302454722018G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host CellsHupB-1TTAGGAAGGGAAGGAAGATAGAGAGTAGGTCGTCGGAGGCAGGGG445'-GTCTTGACTAGTTACGCC-N3-41X-TCATTCAGTTGGCGCCTC-3' (*N = 3nt sequence tag and **X = 41nt random region)ssDNAMycobacterium Tuberculosis (M.Tb.)Histone-like protein HupB (Rv2986c)Identify aptamers that recognise and inhibit the DNA-binding activity of HupB, and significantly block Mtb entry into THP-1 monocytic cells.N/A8Isothermal Titration Calorimetry (ITC)N/ATherapeuticsSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/APatent application no. 20171100124
ABdb_1090 302454722018G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host CellsHupB-2TTAGTAAAGGGGGTAAGAATCAATTGCGAGGTGGAGTGGGAGTGG445'-GTCTTGACTAGTTACGCC-N3-41X-TCATTCAGTTGGCGCCTC-3' (*N = 3nt sequence tag and **X = 41nt random region)ssDNAMycobacterium Tuberculosis (M.Tb.)Histone-like protein HupB (Rv2986c)Identify aptamers that recognise and inhibit the DNA-binding activity of HupB, and significantly block Mtb entry into THP-1 monocytic cells.N/A8Isothermal Titration Calorimetry (ITC)N/ATherapeuticsSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/APatent application no. 20171100124
ABdb_1091 302454722018G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host CellsHupB-4TTAGGTGATTGCTGGAGTGGGTAGCAGATGGGGGGGGGGTTCTTGG455'-GTCTTGACTAGTTACGCC-N3-41X-TCATTCAGTTGGCGCCTC-3' (*N = 3nt sequence tag and **X = 41nt random region)ssDNAMycobacterium Tuberculosis (M.Tb.)Histone-like protein HupB (Rv2986c)Identify aptamers that recognise and inhibit the DNA-binding activity of HupB, and significantly block Mtb entry into THP-1 monocytic cells.HupB-4T treated bacteria yielded reduced intracellular CFU by ∼57.8%.8Isothermal Titration Calorimetry (ITC)1.72 ± 0.00002 μM,TherapeuticsSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledNo loss in the viability of THP-1 cells was observed on exposure to aptamers.In fetal bovine serum (FBS) for 3 hr at 37°C, no change was observed in the integrity of aptamer bands.Best CandidateN/APatent application no. 20171100124
ABdb_1092 302454722018G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host CellsHupB-13TTTTGGGAAGGGGGGGGAGGAAGTTCGATGGTGTTGTGGACCGGG445'-GTCTTGACTAGTTACGCC-N3-41X-TCATTCAGTTGGCGCCTC-3' (*N = 3nt sequence tag and **X = 41nt random region)ssDNAMycobacterium Tuberculosis (M.Tb.)Histone-like protein HupB (Rv2986c)Identify aptamers that recognise and inhibit the DNA-binding activity of HupB, and significantly block Mtb entry into THP-1 monocytic cells.HupB-13T treated bacteria yielded reduced intracellular CFU by ∼45.5%.8Isothermal Titration Calorimetry (ITC)0.17 ± 0.00000005 μMTherapeuticsSubtractive SELEX5'-Biotinylated and 5'-FAM LabeledNo loss in the viability of THP-1 cells was observed on exposure to aptamers.In fetal bovine serum (FBS) for 3 hr at 37°C, no change was observed in the integrity of aptamer bands.Best CandidateN/APatent application no. 20171100124
ABdb_1095 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-01ATACCAGCTTATTCAATT-GGTCGTGTGGACTTGCGATTTCGGTTTGGTGTGGTTGGTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1096 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-02ATACCAGCTTATTCAATT-GGGCATGTGGACTTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.VFCA-02 and VFCA-03 was linear in the range of 4 × 10(1) to 4 × 10(5) CFU/mL of target cell.9Surface Plasmon Resonance (SPR)1.28 × 10(-10) MBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1097 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-03ATACCAGCTTATTCAATT-GGGCATGTGGGCTTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.VFCA-02 and VFCA-03 was linear in the range of 4 × 10(1) to 4 × 10(5) CFU/mL of target cell.9Surface Plasmon Resonance (SPR)1.25 × 10(-9) MBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1098 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-04ATACCAGCTTATTCAATT-TGGCATGTGGACTTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1099 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-05ATACCAGCTTATTCAATT-GGGCATGTGGACTTGCGGTTTCGGTTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1100 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-06ATACCAGCTTATTCAATT-GGGCGTGTGGACTTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1101 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-07ATACCAGCTTATTCAATT-GGGCATGTGGACTTGCGATTTCGGTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1102 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-08ATACCAGCTTATTCAATT-GGGCATGTGGACTTGCGACTTCGGTTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1103 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-09ATACCAGCTTATTCAATT-AGACAGCATAGCACTGTAACGATTGGTTTGGTGTGGTTGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1104 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-10ATACCAGCTTATTCAATT-CCAGAATTGGTGGGTCGACTGCTGGTGTCCTATAAAGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1105 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-11ATACCAGCTTATTCAATT-CGTGGGCGGTAGAGCCAATGCTACTGGAGCGCGTATCCTA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1106 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-12ATACCAGCTTATTCAATT-GTCGGAGAGCCCGGCCGTCTGCTCGTAAGTACTATCATAGC-AGATAGTAAGTGCAATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1107 295537042018Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeriVFCA-13ATACCAGCTTATTCAATT-GCGGACGGATAGTAAATAGCCCATGTACGCTGCTGGCGTC-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Fischeri (ATCC 49387)Whole cellIdentify aptamers against Vibrio fischeri and develop an aptamer-based paper strip sensor for its detection.N/A9Surface Plasmon Resonance (SPR)N/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1108 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt1AGGGTTGGTCGTCCAGCATTCCGTTCGATTGTAGTTCTGACTCTTGTGAAGTTAATGAGCTCCTTTTGCTGACTGTTGTT805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1109 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt2TTCTGATGAGAGTTGCGACCTCCCGGATGAGTGCCCTGGCTCCGGGTTTGTCTATTTTTGGGGGTGTTGACAGATATCCT805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.Vapt2 could detect the pathogen in a wide concentration range: 8–2.0 × 10^8 cfu/ml.The Limit of Detection (LOD) was 8 cfu/ml.13Fluorescence Microscopy26.8 ± 5.3 nMDetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1110 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt3ATCGGATATGAGGCCGGGTATTAAGTGGGGTTCCATATGCAAGGCAGATCTCCCAGTGTTTTTTCAGGATTGCGTTACTG805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_1111 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt4AAGAGGGCTTGGGAACTCTAGGCGTCTGTGCATTAAGCCATGTCTGCCGGGAAAGATCTGTGAAGTGTTCGCCCGCACTT805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1112 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt5GCATGGGTATAGGGAAACCCGAGTCTAGTTTACACTGACGTTAGGAGATTACGTCTGCCGACAGAACATTCTCTCTGTGA805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1113 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt6GGCGGGGTGTTATGAATCCACCGCGATCCGTTATGTTAGGCTGTAATCATTAAGCACTTTATGTGCACTCGTGGTATGCC805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1114 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt7AATTACTGGAGCTTGGCCGTAGGTAGAAAGATTTGCATTATACCTGGGGGGCTCTCTGGTTGTGTTTAATTGGTTACCCC805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1115 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt8ATGCTGCACTAGTTACCTTGCCTTTGCTTTCTTATTCTGCTCGTGCGTAAAAGTGGTTTTTCTTGCGTTGCGGGTGCTTG805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1116 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt9TGTTCGTTCGACTTCTCCGTCGTTTTTGTATTAACGTAGACCTTGGTTGAGCATGCTTACCTCTGCTTGGATTAATGACG805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1117 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt10TAAGGATCTTGGGTTCATGCCGCAACTGGGATGTTTGCTGCCTCCTTTATGGAGCTTAGCTGCGAGCGTTCTTGTTTGGT805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1118 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt11GTCTAAGTGCTTCTAGGCGGATTTGGCGCGTTCCGACGATTAGACTAGGACAACGATTCAGTGGTGTCGATGGTGTGTTC805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1119 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt12GGGGTAAGCCCGCGGCTCTCTCCTATGTATTCAGTGTACTCACGAAGATGTCCGTACTCCGTACGCTGCTATGCGTTCCC805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1120 302705412018Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCRVapt13TCGGGGGCGCAGCTTGGGCCGTTGCTCCTGCCGGTCTCTGTGTGCGTGCGGTTCTCGCTCTTGTGGCTCCGTCCTTGGGG805'-GGCGAAACATCTT-N80-TAGTGACGGTAAGCTTGGCAC-3'ssDNAVibrio VulnificusWhole cellIdentify an aptamer for the detection of V. vulnificus.N/A13Fluorescence MicroscopyN/ADetectionSELEXN/AN/AN/AN/AN/AN/A
ABdb_1126 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS1GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGCTGGTGGCCATTGGCTCGTGTCTCGTTACTGCCGAGTG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.Exhibited a linear concentration ranging from 2×10(1) to 2×10(5) CFU/mL and LOD of 20 CFU/mL.10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1127 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS2GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GGAATGACCTGGATTGACCCGTGAGTGTAGCATTTGTCCG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1128 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS3GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TTGACGATACGCAGGGGCGATACAGACAGTCTGCGTATTC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1129 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS4GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CTGTATCCCTCCTCGCTTCCATACCCAAACCGGACACACC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1130 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS5GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CTGCATGCACCTCACGCATCGAGCACTCACTCCTTCTACG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1131 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS6GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CCTCCCGAGGACACACACACACCATCGCGGTACTCTCCCG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1132 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS7GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GCGCACCTCCGCCCTGTCGGGCGTCGTTTCCCCACGAATC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1133 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS8GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GTTTCCGTATGCGCAGCGCCATACACCTGTCTGATCTCCCC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1134 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS9GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGTCGCAAGGACTCGATCTGGTGGTACCTGCTTTCCGTTG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1135 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS10GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGAGCACCAAGGATAGTTAGCGGTCGCCTTCACACTAGGC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1136 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS11GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CTGACAGATTAAAAGTGGTTGGGCAACTTCTGCTTGCGAA-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)4.41 × 10(-12) MDetectionWhole Cell-SELEX5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1137 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS12GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GGTGGTGTTGACAATTGAACCTTTGCAGGGGTCTGGTGGG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1138 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS13GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GTGGAGGTGCGCTATAGGTCTCTCCGGGTACTGATCCAAT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1139 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS14GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CCGACCCGTAACGCCAGTGAGGGAACACTCTGGAATAGTT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1140 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS15GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CGAGCAAACTGATAAACTCCGCCATGTCAGGCAAAGCTTT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1141 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS16GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CTTCGTTCCGTTGACATAATTCAATTTTGCGTATTTCTTG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1142 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS17GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-ATGGGGCTAGTCTACTTCTGTGTCTACCTAGGATTCCGCT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1143 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS18GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGTAGGCGTAACCTCCTACTCTGTGCACTGTCTAAAGTGAC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1144 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS19GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CGCGGCGTACGCTATCTTGTAACCTCCCGAACTATAACCAC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1145 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS20GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CAAGCGACTCGACCGTGGTACCTCCCCAATAGCCATTTGA-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1146 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS21GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TCTCGCAGCCTCTTCCAATCTTGTTATTTACGCTTTCTGT-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1147 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS22GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CACAAAGGAGCAAGAACTCAATTGCGCTCGTACCTTGTGG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1148 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS23GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GTAGCTGGGGTCCGGATCTGCGGTTCAGTGCGCTATTGTG-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1149 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS24GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGACGGCTATCTCATTTGGCACATCATTTAGTAAGCTATC-AGATAGTAAGTGCAATCT1005'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)3.75 ×10(-3) MDetectionWhole Cell-SELEX5'-Cyanine5 (Cy5) Labeled or 5′-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1152 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#1ATACCAGCTTATTCAATT-CCATGGTCCCTCGTGTTTATTATGTTGTCTGAACTGGCTG-AGATTGCACTTACTATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.VPCAapta#1 was significantly higher (798.41 ng/ul) than that of the other aptamer.11Surface Plasmon Resonance (SPR)2.04 ± 0.12 nMDiagnosticWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1153 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#2ATACCAGCTTATTCAATT-CCACGTTTCCACGTATCCTCGGAGCTTGCTTAACCGTACG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1154 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#3ATACCAGCTTATTCAATT-TGCGTAGTTCTTTTAACAACCATTCAGCGTATTTTCGTGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1155 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#4ATACCAGCTTATTCAATT-CCCTCGGCGTAACTAGTACCGTTGCACCACCAACGTGATT-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1156 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#5ATACCAGCTTATTCAATT-CGCAAGGGATTTCGGACCGGGCTTGGTCACACACAGAGCA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1157 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#6ATACCAGCTTATTCAATT-CCAGCAGTGGGGCATGGGGGAACGATAAGATTATAAGGGGG-AGATAGTAAGTGCAATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1158 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#7ATACCAGCTTATTCAATT-CACGCAAGCAGAAGCCACTCCCCCTGGTCGTACTATTTAG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1159 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#8ATACCAGCTTATTCAATT-CACAGGCGTACCACTGCCCCAACGATCCTTTTGGTTACCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1160 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#9ATACCAGCTTATTCAATT-GTCGGAGAGCCTGGCCGTCTGCTCGTAAGTACTATCATAGC-AGATAGTAAGTGCAATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1161 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#10ATACCAGCTTATTCAATT-TGGAGAAGCGGCAGTTGATGCCTGGTACCCGTCTGCTACG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1162 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#11ATACCAGCTTATTCAATT-CGTAAAGGACGCTGCCATGAATGTGCTATCCAAGCCTGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1163 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#12ATACCAGCTTATTCAATT-CGTAAAGAACGCTGCCATGAATGTGCTATCCAAGCCTGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1164 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#13ATACCAGCTTATTCAATT-CGTAAGAACGCTGCCATGAATGTGCTATCCAGGCCTGGG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1165 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#14ATACCAGCTTATTCAATT-CAACGGAGGGAAGTTTCCATGTCCGGTACCAAGCGGTTTTTG-AGATAGTAAGTGCAATCT785'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1166 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#15ATACCAGCTTATTCAATT-GCACGGGAGGCACCCTGACATTGCAAATCCCATCGGTGTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1167 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#16ATACCAGCTTATTCAATT-ACCTATAACAGCCATCATACGAGGTAATCCCCTCCCTCGA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1168 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#17ATACCAGCTTATTCAATT-CCAACTGGATCTTACTGAGGAACGGTCCATTAGCACATCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1169 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#18ATACCAGCTTATTCAATT-CGCGACCACATCCTGCGTAAACACATCTCCGCCAGTCCAT-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1170 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#19ATACCAGCTTATTCAATT-CGCAAGAGCAGCATACAGCACGAACGAGCCATTTACGCCA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1171 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#20ATACCAGCTTATTCAATT-CGAAGACCAGACATGGTAGCCACGCATAGTCCAACTTAAG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1172 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#21ATACCAGCTTATTCAATT-ACACCACACTAATGCATGCATTCTGTGAGTCACAGTTCA-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1173 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#22ATACCAGCTTATTCAATT-CACTCAAACCCAAACCATGCAAACTGAACAACGCAACACA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1174 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#23ATACCAGCTTATTCAATT-CACCAAACTGCCCAAATTTGAGGACGCCCTATACATGCG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1175 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#24ATACCAGCTTATTCAATT-GCCCAATACGTATGTTGATACATGCCCCTTACCCTTTGCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1176 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#25ATACCAGCTTATTCAATT-CACCGTAGTAAGTAAGCAGACGCTAAGGATGTTGCCAGTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1177 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#26ATACCAGCTTATTCAATT-GTGACATGAACTGGTTTTCCACAGCTTAGGATCATGGATG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1178 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#27ATACCAGCTTATTCAATT-GGGCATGTGGGCTTGCGATTTCGGTTTGGTGTGGTTGGGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1179 294486352018Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticusVPCA_Apta#28ATACCAGCTTATTCAATT-CCGTCGCTATAACCCTGTCTTTATATATCTTCGCCCACGGG-AGATAGTAAGTGCAATCT775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIsolate specific aptamers against Vibrio parahaemolyticus and develop an SPR biosensor for its detection.N/A11Surface Plasmon Resonance (SPR)N/ADiagnosticWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1183 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC5CCTAACCGATATCACACTCAC-GGCTAGCGGACAGGTGTAGGTCGGAATCCTTCAGCGTAGA-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1184 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC7CCTAACCGATATCACACTCAC-AGTATACCGCTCCACCAGTGTGATATCGGGATCTGCTGAC-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1185 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC10CCTAACCGATATCACACTCAC-GTAGTGAGGTGGGTCGTGAAGGGAGGCACTACTGGTGCGT-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1186 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC13CCTAACCGATATCACACTCAC-GAGGTGAGGGTGCGAGGGTAAGGGGGCAAACCTGGTGCGT-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1187 302364832018Staggered Target SELEX, a novel approach to isolate non-cross-reactive aptamer for detection of SEA by apta-qPCRC16CCTAACCGATATCACACTCAC-ACGTAGTGAGGGTTGCGAGGGTAAGGTGGTCAGTATACGC-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-40N-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers by ST-SELEX and use them for the detection of SEA via apta-Real-time PCR (apta-qPCR).The limit of detection (LOD) was 146.67 fM.10Surface Plasmon Resonance (SPR)7.44 ± 0.6 nMDetectionStaggered Target SELEX (ST-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1203 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G1CAGGTCCATCGAGTGGTA-GGAGGTATTTAGTGCCAAGCCATCTCAAACGACGTCTGAG-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)3.1 ± 1.2 nMDetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1204 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G3CAGGTCCATCGAGTGGTA-TGCAGCGGACAGTGTGGGACCATCGCTGCGGATGTATGAA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)5.6 ± 2.4 nMDetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1205 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G7CAGGTCCATCGAGTGGTA-CCCAACCCACGATGCGCAAGAGGAATGCAGCCTACCAGCA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)4.6 ± 1.6 nMDetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/ABest CandidateN/AN/A
ABdb_1206 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G1-T1TAGGAGGAGGTATTTAGTGCCAAGCCATCTCAAACGACGTCTGAGTCGCACTGCTCCTG595'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.Achieved a detection limit of 1 nM.10Electrophoretic Mobility Shift Assay (EMSA)4.5 ± 2.2 nMDetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled) and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1207 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G2CAGGTCCATCGAGTGGTA-CCGAGTTCCCAATATTATGGCTATGCAGGATACTTCACCT-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1208 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G4CAGGTCCATCGAGTGGTA-TGAGTACTAGTTCCCCAGGAGAAAGCAGATCCCCAGGTAC-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1209 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G5CAGGTCCATCGAGTGGTA-GCACAGGACGCAAGATGAATGCAGCATACCAGTCCCTAGA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1210 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G6CAGGTCCATCGAGTGGTA-CAGCTGTCGACGCGTTACCGTGAACGGAACACCGATGACG-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1211 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G8CAGGTCCATCGAGTGGTA-TGCGTGATTGGACCAGGGAAAGATGCACCGCAAGACAAGA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1212 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G9CAGGTCCATCGAGTGGTA-AGGATAATCCGATACGCAAGAAGAAAGCAGATTACCAGGA-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1213 288826272018Selection and characterization of DNA aptamers for detection of glutamate dehydrogenase from Clostridium difficileanti-G10CAGGTCCATCGAGTGGTA-CAAAGTCGGCAAGGTGGAAAGCAGCCACACCACGACTAGT-TCGCACTGCTCCTGAACGTAC795'-CAGGTCCATCGAGTGGTAGGA-N40-TCGCACTGCTCCTGAACGTAC-3'ssDNAClostridium DifficileGlutamate dehydrogenase (GDH)Identify anti-GDH DNA aptamers and develop a structuring-switching aptamer sensor for its detection.N/A10Electrophoretic Mobility Shift Assay (EMSA)N/ADetectionSELEX5'-Radiolabelled ([γ-32P]-ATP-labeled)N/AN/AN/AN/AN/A
ABdb_1214 317045872019Theranostic Application of a Novel G-Quadruplex-Forming DNA Aptamer Targeting Malate Synthase of Mycobacterium tuberculosisMS10-TruncGGTGGTGGTGG115'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (H37Rv)Surface-associated Malate synthase (MS -Whole cells)Identify aptamers that bind to and inhibit the MS enzyme activity, thereby blocking bacterial entry into host cells.Inhibited MS activity with an IC₅₀ of 251.1 nM; Ki of 230 nM; inhibited M. tb invasion into human THP-1 monocytic cells.10Surface Plasmon Resonance (SPR)1.9 × 10−11 MTherapeuticsSELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AIndian Patent application no. 201611021901
ABdb_1215 317045872019Theranostic Application of a Novel G-Quadruplex-Forming DNA Aptamer Targeting Malate Synthase of Mycobacterium tuberculosisMS4GGACCAGGTGAGTGGATGCGGGTGCGGGTGGGGGTGAGGGGGGG445'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (H37Rv)Surface-associated Malate synthase (MS -Whole cells)Identify aptamers that bind to and inhibit the MS enzyme activity, thereby blocking bacterial entry into host cells.Inhibition of MS activity, the aptamers were ranked as MS10 (IC50 of 275.6 nM, Ki of 270 nM) > MS5 > MS20 > MS4 > MS6.10Surface Plasmon Resonance (SPR)9.9 × 10−9 MTherapeuticsSELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1216 317045872019Theranostic Application of a Novel G-Quadruplex-Forming DNA Aptamer Targeting Malate Synthase of Mycobacterium tuberculosisMS5GCAGAGAGAGAGAAAGTGAGAAGGGCGGGGGGGAGAGGGTGGGG445'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (H37Rv)Surface-associated Malate synthase (MS -Whole cells)Identify aptamers that bind to and inhibit the MS enzyme activity, thereby blocking bacterial entry into host cells.Inhibition of MS activity, the aptamers were ranked as MS10 (IC50 of 275.6 nM, Ki of 270 nM) > MS5 > MS20 > MS4 > MS6.10Surface Plasmon Resonance (SPR)2.7 × 10−6 MTherapeuticsSELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1217 317045872019Theranostic Application of a Novel G-Quadruplex-Forming DNA Aptamer Targeting Malate Synthase of Mycobacterium tuberculosisMS6GGTGGGATGGGGGGGGGAGGGTAGGGTGTGCAAGTAGAGGGTC435'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (H37Rv)Surface-associated Malate synthase (MS -Whole cells)Identify aptamers that bind to and inhibit the MS enzyme activity, thereby blocking bacterial entry into host cells.Inhibition of MS activity, the aptamers were ranked as MS10 (IC50 of 275.6 nM, Ki of 270 nM) > MS5 > MS20 > MS4 > MS6.10Surface Plasmon Resonance (SPR)2.8 × 10−10 MTherapeuticsSELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1218 317045872019Theranostic Application of a Novel G-Quadruplex-Forming DNA Aptamer Targeting Malate Synthase of Mycobacterium tuberculosisMS10GGTGTGTTGACTGAGGGGGTGGGGTGGGTGGTGGTGGATATAGC445'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (H37Rv)Surface-associated Malate synthase (MS -Whole cells)Identify aptamers that bind to and inhibit the MS enzyme activity, thereby blocking bacterial entry into host cells.Inhibition of MS activity, the aptamers were ranked as MS10 (IC50 of 275.6 nM, Ki of 270 nM) > MS5 > MS20 > MS4 > MS6.10Surface Plasmon Resonance (SPR)1.2 × 10−8 MTherapeuticsSELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1219 317045872019Theranostic Application of a Novel G-Quadruplex-Forming DNA Aptamer Targeting Malate Synthase of Mycobacterium tuberculosisMS20GGGTTGTAAGAGGGAGCTGGGAGGGGTCGGGGGGGGGGGTGGC435'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (H37Rv)Surface-associated Malate synthase (MS -Whole cells)Identify aptamers that bind to and inhibit the MS enzyme activity, thereby blocking bacterial entry into host cells.Inhibition of MS activity, the aptamers were ranked as MS10 (IC50 of 275.6 nM, Ki of 270 nM) > MS5 > MS20 > MS4 > MS6.10Surface Plasmon Resonance (SPR)9.1 × 10−7 MTherapeuticsSELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1220 307797542019C4-HSL aptamers for blocking quorum sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysisA16CCATCCACACTCCGCAAGTGGGGAGGGGAGAGACGACGATCCTGTGGGTTTTCTGCAGTGAGTCGTGTTTTCGACTTATTGCGTCGGCTGCCTCTACAT995'-CCATCCACACTCCGCAAG-N30-TTTT-hybridsequence-TTTT-N10-CGTCGGCTGCCTCTACAT-3'ssDNAPseudomonas AeruginosaC4-HSL of the rhl system quorum sensing moleculeIdentify aptamers that inhibit biofilm formation and quorum sensing.Biofilm formation by P. aeruginosa was reduced by about 1/3.10Saturation Binding Assay28.47 nMTherapeuticsStructure Switching SELEXN/AAptamers caused no effect on bacterial growth.N/AN/AN/AN/A
ABdb_1221 307797542019C4-HSL aptamers for blocking quorum sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysisA46CCATCCACACTCCGCAAGTATGAGGACAGTTGGAGGGCGCGCCGTGTTTTTTCTGCAGTGAGTCGTGTTTCCTCGCTCACGCGTCGGCTGCCTCTACAT995'-CCATCCACACTCCGCAAG-N30-TTTT-hybridsequence-TTTT-N10-CGTCGGCTGCCTCTACAT-3'ssDNAPseudomonas AeruginosaC4-HSL of the rhl system quorum sensing moleculeIdentify aptamers that inhibit biofilm formation and quorum sensing.Biofilm formation by P. aeruginosa was reduced by about 1/3.10Saturation Binding AssayN/ATherapeuticsStructure Switching SELEXN/AAptamers caused no effect on bacterial growth.N/AN/AN/AN/A
ABdb_1222 307797542019C4-HSL aptamers for blocking quorum sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysisA1CCATCCACACTCCGCAAGTTTTAGGAGTAGTGGTGTGGGGACCGACTCTTTTCTGCAGTGAGTCGTGTTTTCGAGTCATCCCGTCGGCTGCCTCTACAT995'-CCATCCACACTCCGCAAG-N30-TTTT-hybridsequence-TTTT-N10-CGTCGGCTGCCTCTACAT-3'ssDNAPseudomonas AeruginosaC4-HSL of the rhl system quorum sensing moleculeIdentify aptamers that inhibit biofilm formation and quorum sensing.Biofilm formation by P. aeruginosa was reduced by about 1/3.10Saturation Binding AssayN/ATherapeuticsStructure Switching SELEXN/AAptamers caused no effect on bacterial growth.N/AN/AN/AN/A
ABdb_1223 307797542019C4-HSL aptamers for blocking quorum sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysisA2CCATCCACACTCCGCAAGTGAGATGTGGTGTAGGCAACTCGGCATAGATTTTCTGCAGTGAGTCGTGTTTTTTGACACGGCCGTCGGCTGCCTCTACAT995'-CCATCCACACTCCGCAAG-N30-TTTT-hybridsequence-TTTT-N10-CGTCGGCTGCCTCTACAT-3'ssDNAPseudomonas AeruginosaC4-HSL of the rhl system quorum sensing moleculeIdentify aptamers that inhibit biofilm formation and quorum sensing.Biofilm formation by P. aeruginosa was reduced by about 1/3.10Saturation Binding AssayN/ATherapeuticsStructure Switching SELEXN/AAptamers caused no effect on bacterial growth.N/AN/AN/AN/A
ABdb_1226 314037642019Rapid Detection of Mycoplasma-Infected Cells by an ssDNA Aptamer ProbeAptamer A15-1GTGGGGTTGAAAACGCCGGAGAGGGTGTGTGGGTGGGGTA405'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMycoplasma-Infected Cells (Jeko-1 lymphoma cells)Whole cellDevelop an aptamer probe for the rapid detection of mycoplasma-infected cells.Mycoplasma-positive cells showed a strong signal, but no signal was detected in the mycoplasma-negative cells.15Flow Cytometry24.5 nMDetectionWhole Cell-SELEX5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1233 https://doi.org/10.1021/acssuschemeng.9b013142019Development of a Sensitive Diagnostic Device Based on Zeolitic Imidazolate Frameworks-8 Using Ferrocene–Graphene Oxide as Electroactive Indicator for Pseudomonas aeruginosa DetectionP.aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellAn electrochemical biosensor (Fc-GO/Apt/HZIFs-8/GCE ) based on aptamers immobilized in engineered zeolitic imidazolate Framework-8 (ZIFs-8) for the detection of P. aeruginosa in human urine samples.Exhibits a wide linear dynamic range (from 1.2 × 10(1) to 1.2 × 10(7) CFU/mL) with a low detection limit of 1 CFU/mL.N/AN/AN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1238 309501492019Recognition of Helicobacter pylori by protein-targeting aptamersHp1AGTGTGCTCTTCTCAGGTCT-GTGGCCGGGGGCACTAATGCGGGCTATAAGTCTCCTTGGG-GCAGTGTCTCAGCATACGCA805'-AGTGTGCTCTTCTCAGGTCT-40N-GCAGTGTCTCAGCATACGCA-3'ssDNAHelicobacter PyloriH. pylori surface recombinant antigens (HP-Ag)Identify aptamers against H pylori surface recombinant antigens.N/AN/AFluorescence Binding Assay35.60 ± 6.36 nMDetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1239 309501492019Recognition of Helicobacter pylori by protein-targeting aptamersHp2AGTGTGCTCTTCTCAGGTCT-CCTATACCTGGCATCAGAGAGCTAGGGGCCACGGTTCGCA-GCAGTGTCTCAGCATACGCA805'-AGTGTGCTCTTCTCAGGTCT-40N-GCAGTGTCTCAGCATACGCA-3'ssDNAHelicobacter PyloriH. pylori surface recombinant antigens (HP-Ag)Identify aptamers against H pylori surface recombinant antigens.N/AN/AFluorescence Binding Assay204.38 ± 97.31 nMDetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1240 309501492019Recognition of Helicobacter pylori by protein-targeting aptamersHp3AGTGTGCTCTTCTCAGGTCT-GGTGCTGCGATGCTTTCTGGTGGTGTATGGTTGTCTTTTG-GCAGTGTCTCAGCATACGCA805'-AGTGTGCTCTTCTCAGGTCT-40N-GCAGTGTCTCAGCATACGCA-3'ssDNAHelicobacter PyloriH. pylori surface recombinant antigens (HP-Ag)Identify aptamers against H pylori surface recombinant antigens.N/AN/AFluorescence Binding AssayN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1241 309501492019Recognition of Helicobacter pylori by protein-targeting aptamersHp4AGTGTGCTCTTCTCAGGTCT-CGGCGCGGTTGTGGGTACCTAGGGTTGTTGTTGCTTCTCA-GCAGTGTCTCAGCATACGCA805'-AGTGTGCTCTTCTCAGGTCT-40N-GCAGTGTCTCAGCATACGCA-3'ssDNAHelicobacter PyloriH. pylori surface recombinant antigens (HP-Ag)Identify aptamers against H pylori surface recombinant antigens.Hp4 had excellent binding to H pylori cells and almost no binding to E coli, S aureus, or V anguillarum.N/AFluorescence Binding Assay26.48 ± 5.72 nMDetectionSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1242 309501492019Recognition of Helicobacter pylori by protein-targeting aptamersHp5AGTGTGCTCTTCTCAGGTCT-GGGCTGTGGGTCGGCACGTCGTCTCTTCATGGTTGTGGTG-GCAGTGTCTCAGCATACGCA805'-AGTGTGCTCTTCTCAGGTCT-40N-GCAGTGTCTCAGCATACGCA-3'ssDNAHelicobacter PyloriH. pylori surface recombinant antigens (HP-Ag)Identify aptamers against H pylori surface recombinant antigens.N/AN/AFluorescence Binding AssayN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1243 309501492019Recognition of Helicobacter pylori by protein-targeting aptamersHp6AGTGTGCTCTTCTCAGGTCT-TTAGGGACCCATGGGCTAACCCGGGCACAAGATTGTCTCA-GCAGTGTCTCAGCATACGCA805'-AGTGTGCTCTTCTCAGGTCT-40N-GCAGTGTCTCAGCATACGCA-3'ssDNAHelicobacter PyloriH. pylori surface recombinant antigens (HP-Ag)Identify aptamers against H pylori surface recombinant antigens.N/AN/AFluorescence Binding AssayN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1244 309501492019Recognition of Helicobacter pylori by protein-targeting aptamersHp7AGTGTGCTCTTCTCAGGTCT-ACACAACCTGCCGTATTGTAACCGTCGTCCCCCCGAAGCA-GCAGTGTCTCAGCATACGCA805'-AGTGTGCTCTTCTCAGGTCT-40N-GCAGTGTCTCAGCATACGCA-3'ssDNAHelicobacter PyloriH. pylori surface recombinant antigens (HP-Ag)Identify aptamers against H pylori surface recombinant antigens.N/AN/AFluorescence Binding AssayN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1258 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA3PATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.Exhibits a wide linear detection range (from 10(2) to 10(7) cfu/mL), and the detection limit could be as low as 10 cfu/mL.N/AFlow Cytometry50.76 ± 8.92 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1259 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry32.53 ± 6.86 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1260 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA2GCAAAGAAACAGTGACTCGTTGA23N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry37.44 ± 6.98 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1261 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA3GCAACGAAACAGTGACTCGTTGA23N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry34.61 ± 3.47 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1262 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4CAACGAAACAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry28.65 ± 3.05 nMBiosensorWhole Cell-SELEX5'-FAM Labeled and 5'-Biotinylated (Biotin-TTTTTTTTT)N/AN/ABest CandidateN/AN/A
ABdb_1263 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA5AACGAAACAGTGACTCGTT19N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry422.0 ± 21.93 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1264 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA6ACGAAACAGTGACTCGT17N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry412.9 ± 27.12 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1265 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-1CAACGAAACATTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1004 ± 187.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1266 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-2CAACGAAACAGTTACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1103 ± 180.2 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1267 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-3CAACGAAACTGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry958.9 ± 183.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1268 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-4CAACGAAACAGCGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1070 ± 73.05 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1269 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-5CAACGAAACAGTGTCTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1119 ± 187.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1270 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-6CAACGAAATAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1070 ± 125.4 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1271 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-7CAACGAACCAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry423.2 ± 36.91 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1272 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-8CAACGATACAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry467.0 ± 33.38 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1273 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-9CAACGAAACAGTGAGTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry376.4 ± 54.25 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1282 315116142019Detection of Gram-negative bacterial outer membrane vesicles using DNA aptamersGN6ATACCAGCTTATTCAATT-GGGTGAGGGGGGGTTCACAACGTTAAAGATAGACGGGGGA-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAGram-negative Bacteria (Escherichia Coli (E. Coli) DH5α, Escherichia Coli (E. Coli) K12, and Serratia Marcescens)Whole cellIsolated aptamers against multiple Gram-negative bacterial species and developed an aptamer-based detection tool (ELAA) towards bacterial secretory cargo released from the outer membranes of Gram-negative bacteria.Can detect as low as 25 ng/mL of bacterial OMVs, and dissociation constants of GN6 to OMVs derived from E. coli DH5α, E. coli K12 and S. marcescens were 0.13 ± 0.01 μg/ml, 3.70 ± 0.98 μg/ml and 0.23 ± 0.16 μg/ml, respectively.12Fluorescence Binding Assay29.94 ± 2.49 nM (for E. coli DH5α), 59.70 ± 10.89 nM (for E. coli K12) and 38.98 ± 6.46 nM (for S. marcescens)DetectionSequential Toggle Cell-SELEX (STC-SELEX)5'-Biotinylated and 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1283 315116142019Detection of Gram-negative bacterial outer membrane vesicles using DNA aptamersGN12ATACCAGCTTATTCAATT-CCGAGTCCAGACTCACCGCCGCCTCCTCAAGACGTGCTGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAGram-negative Bacteria (Escherichia Coli (E. Coli) DH5α, Escherichia Coli (E. Coli) K12, and Serratia Marcescens)Whole cellIsolated aptamers against multiple Gram-negative bacterial species and developed an aptamer-based detection tool towards bacterial secretory cargo released from the outer membranes of Gram-negative bacteria.GN12 was 3.6 times higher in binding to 10(8) cells of Gram-negative bacteria than to Gram-positive bacteria tested.12Fluorescence Binding Assay20.36 ± 2.38 nM (for E. coli DH5α), 24.80 ± 3.98 nM (for E. coli K12) and 53.83 ± 17.70 nM (for S. marcescens)DetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1284 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA1GACGCTTACTCAGGTGTGACTCG-TCCATACCTCCTATTCCTATCTGATCACCATGAGCTCTCGCCTGAACTGG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1285 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA2GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.VA2 could recognize different V. alginolyticus prevalent in Beibu Gulf with high specificity and affinity.9Flow Cytometry14.31 ± 4.26 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledNo cytotoxic effects shown in vitro and in vivo.N/ABest CandidateN/AN/A
ABdb_1286 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA3GACGCTTACTCAGGTGTGACTCG-TATCTTGTCAGCATATAGCCGCCCTGTCTCGTGCCTCTAATTGGGCTTGT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1287 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA4GACGCTTACTCAGGTGTGACTCG-CTTGGTGGGGGGTGGCGGGTTGGTGATTAGTTGCGTGTACAACCGTTGCG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1288 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA5GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1289 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA6GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1290 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA7GACGCTTACTCAGGTGTGACTCG-GGGGCACTCTGGGGAAGGACGTAAGCGATAGCCGACCTCGTGGAATATTA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1291 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA8GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.VA8 could recognize different V. alginolyticus prevalent in Beibu Gulf with high specificity and affinity but less potent than VA2.9Flow Cytometry90.00 ± 13.51 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledNo cytotoxic effects shown in vitro and in vivo.N/ABest CandidateN/AN/A
ABdb_1292 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA9GACGCTTACTCAGGTGTGACTCG-TCACCGTCATACACGGTCACCTGTTCTGCTATCACCCTGACGTGATTGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1293 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA10GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1294 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA11GACGCTTACTCAGGTGTGACTCG-TGGCGCAAGGGAAGGTGTTGGGGGATGGTTGGTGGGGTGTGTGGTTGTAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1295 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA12GACGCTTACTCAGGTGTGACTCG-ATGGCTGACTATATAAGAGAACGGGGGCTGCGGTGGTCCCGATCTGGCAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1296 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA13GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1297 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA14GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1298 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA15GACGCTTACTCAGGTGTGACTCG-TGGCGATGTACGCACCGCCCTCAGACTCATTCAGCATATAGCCTAGTAAC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1299 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA16GACGCTTACTCAGGTGTGACTCG-GGGGTGGATTGGGTGGGGTGGTTGGTTCGTTTTAAAGTACTGTGTAAGCG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1300 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA17GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1301 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA18GACGCTTACTCAGGTGTGACTCG-GCTGCGTTAACATATAGGGCATTTGAGTGCGCGCATAGGGTTGTGGCGAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1302 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA19GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1303 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA20GACGCTTACTCAGGTGTGACTCG-TCCAGTCAGTATATAACTCCTCGACCCTGTGATCGGCTACGGTGGCAGTA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1304 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA21GACGCTTACTCAGGTGTGACTCG-GAGTGCTCGGTTGGTGTTGGGGGAGGGTGGTGGGTGACCATGCTACATGT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1305 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA22GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1306 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA23GACGCTTACTCAGGTGTGACTCG-GGGCGGGTGGACGGGGGTGGTATGGAGGTTGGAGTAAGCGGTTTCACCGA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1307 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA24GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1308 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA25GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1309 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA26GACGCTTACTCAGGTGTGACTCG-TCTGGTAGCATATAGCCGGCAGAGCACGGTCCCCTCATGGTTGGCACGTA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1310 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA27GACGCTTACTCAGGTGTGACTCG-GCCATGCTCACTATATAAGATATGTAAGTTGGACTACATATGTATGGCTG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1311 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA28GACGCTTACTCAGGTGTGACTCG-GCTGTGATCGTATGGACGCTGCAGTTGGATGACGCTATGAGGTATTCCAA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1312 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA29GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1313 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA30GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1314 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA31GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1315 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA32GACGCTTACTCAGGTGTGACTCG-CACTATTGTCAAGTCACGAGTTAGGTACCTATGTGATCTGAGGTATCAA-CGAAGGACGCAGATGAAGTCTC945'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1316 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA33GACGCTTACTCAGGTGTGACTCG-CTACTCAGGTGACCCTCAAACGTATCTAGTTTGGCAGCGAGGTTCCTTCG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1317 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA34GACGCTTACTCAGGTGTGACTCG-TTGCGAATATAGCGACGAGTATCGTCAGAGTCGCCGGGTCCAGGTTCGA-CGAAGGACGCAGATGAAGTCTC945'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1318 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA35GACGCTTACTCAGGTGTGACTCG-GGTGGTGGGGATTTGGGGTGGTTGGTTCATCTATCTGGTCGTTACCGGGG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1319 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA36GACGCTTACTCAGGTGTGACTCG-CGCGGCACCAGCACGACGGTTCGATTGGCTGCGGAAACCACGCACTGCAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1320 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA37GACGCTTACTCAGGTGTGACTCG-GGTGTTGGTGGGTGGCGGGGTGGTGGTTGGTGTTCCCGAACCTGATGAAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1321 315633152019Rapid identification and quantitation of the viable cells of Lactobacillus casei in fermented dairy products using an aptamer-based strategy powered by a novel cell-SELEX protocolApt-1ATCGTATACCTAGAAGATATGACCGGCAGTTGATGAGATAGAGGCGCTGGTGGGTAGATA605'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNALactobacillus Casei (ATCC 393)Whole cellAn aptamer-based strategy using PEG and chitosan-modified graphene oxide, with complementary ring-mediated RCA, was developed for qualitative and quantitative detection of viable L. casei in dairy products.N/A8Flow Cytometry21.1 ± 2.7 nMDetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1322 315633152019Rapid identification and quantitation of the viable cells of Lactobacillus casei in fermented dairy products using an aptamer-based strategy powered by a novel cell-SELEX protocolApt-2GGCTCACCTACAGGCTGCGGAGTCATAGCATCGTGACAGAGTCGAGTGTCGACTATACGT605'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNALactobacillus Casei (ATCC 393)Whole cellAn aptamer-based strategy using PEG and chitosan-modified graphene oxide, with complementary ring-mediated RCA, was developed for qualitative and quantitative detection of viable L. casei in dairy products.Achieved a detection limit of 10(5) cfu/mL and selective detection of L. casei in commercial dairy drinks, with a dynamic range of 10(5) to 10(9) cfu/mL.8Flow Cytometry19.3 ± 3.7 nMDetectionWhole Cell-SELEX3'-Biotinylated (biotin-AAAAAAAAAA)N/AN/AN/AN/AN/A
ABdb_1323 315633152019Rapid identification and quantitation of the viable cells of Lactobacillus casei in fermented dairy products using an aptamer-based strategy powered by a novel cell-SELEX protocolApt-3TCGAAGTGAGCGCGCGGTGTGGTGACTGTGTTGCAGATGGATGCATGGAGGTGATGATGA605'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNALactobacillus Casei (ATCC 393)Whole cellAn aptamer-based strategy using PEG and chitosan-modified graphene oxide, with complementary ring-mediated RCA, was developed for qualitative and quantitative detection of viable L. casei in dairy products.Achieved a detection limit of 10(5) cfu/mL and selective detection of L. casei in commercial dairy drinks, with a dynamic range of 10(5) to 10(9) cfu/mL.8Flow Cytometry15.6 ± 4.1 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1341 https://doi.org/10.1016/j.snb.2018.12.1122019Screening of highly-specific aptamers and their applications in paper-based microfluidic chips for rapid diagnosis of multiple bacteriaA1ACAGCACCACAGACCACATATCACATGCTGTCGCCTTGCGATATCAATTCCAGTGATGTTTGTCTTCCTGCC725'-GGCAGGAAGACAAACA-N40-TGGTCTGTGGTGCTGT-3'ssDNAAcinetobacter BaumanniiWhole cellIdentify aptamers and develop a dual-aptamer, NC-based microfluidic chip for fast diagnosis of three common nosocomial bacteria.LOD was estimated to be 10(3) for AB.3Fluorescence Spectroscopy6.8 ± 1.9 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1342 https://doi.org/10.1016/j.snb.2018.12.1122019Screening of highly-specific aptamers and their applications in paper-based microfluidic chips for rapid diagnosis of multiple bacteriaE27ACAGCACCACAGACCACAGATCATACCAGTGCGGCCGTTAGCCTCGTTAATCTGGCGTTTGTCTTCCTGCC715'-GGCAGGAAGACAAACA-N40-TGGTCTGTGGTGCTGT-3'ssDNAEscherichia Coli (E. Coli)Whole cellIdentify aptamers and develop a dual-aptamer, NC-based microfluidic chip for fast diagnosis of three common nosocomial bacteria.LOD was estimated to be 10(4) for EC.3Fluorescence Spectroscopy11.9 ± 3.7 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1343 https://doi.org/10.1016/j.snb.2018.12.1122019Screening of highly-specific aptamers and their applications in paper-based microfluidic chips for rapid diagnosis of multiple bacteriaO28GGGGAAGACAAACACCTCATAGTCGGTATCGGCCGTTTGGGCGTTTTTCCGATGGTCTGTGGTGCTGT685'-GGCAGGAAGACAAACA-N40-TGGTCTGTGGTGCTGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers and develop a dual-aptamer, NC-based microfluidic chip for fast diagnosis of three common nosocomial bacteria.LOD was estimated to be 10(5) CFU/μL for MRSA.3Fluorescence Spectroscopy199.6 ± 35.8 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1346 https://doi.org/10.1016/j.foodcont.2018.11.0482019Naked-eyes detection of Shigella flexneri in food samples based on a novel gold nanoparticle-based colorimetric aptasensorSh. flexneri binding aptamerCCGGACTAGGGCTGGTTAGCTTCAATACTGCTGGGCGAGG40N/AssDNAShigella Flexneri (ATCC 12022)Whole cellDeveloped a colourimetric aptasensor based on an aptamer and gold nanoparticles (AuNPs) for the visual detection of Sh. flexneri.Achieved a linear range from 10(2) to 10(6) CFU/mL with the detection limit of 80 CFU/mL, and the detection time was 20 min or less.14N/A42.6 ± 7.11 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1349 319531752020Single-stranded DNA (ssDNA) Aptamer targeting SipA protein inhibits Salmonella Enteritidis invasion of intestinal epithelial cellsApt17TAGGGAAGAGAAGGACATATGAT-GCAATGGAACCGCTGAACGACCCTAGCATTATCAGTGTGG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNASalmonella Enteritidis (S. Enteritidis) TM 6 andTM 68SipA proteinIdentify an aptamer targeting the SipA protein and interfere with the function of effector proteins to prevent host cell invasion.70% and 37.7% inhibition ratios against adhesion and invasion of S. enteritidis TM 6 to Caco-2 cells, 45.71% and 39.5% against those of S. enteritidis TM 68, respectively.9Fluorescence Spectroscopy114.9 nM (at 27°C) and 63.4nM (at 37°C)TherapeuticsMagnetic Bead (MB)-based SELEX3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1367 324995572020Identification of two aptamers binding to Legionella pneumophila with high affinity and specificityR10C5GCAATGGTACGGTACTTCCGGACAGTGCTGAAAACTGTGACCCCCCAAAAGTGCACGCTACTTTGCTAA695'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNALegionella Pneumophila (Lp 120292)Whole cellIdentify an aptamer against Lp and utilise it as a biorecognition element in a biosensor to detect Lp in real-time and in situ.Around 60% of lp120292 cells are stained by R10C5 and 20% of Pseudomonas strains.10Flow Cytometry116 nMBiosensorWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/APatent application no US 16/850,355
ABdb_1368 324995572020Identification of two aptamers binding to Legionella pneumophila with high affinity and specificityR10C1GCAATGGTACGGTACTTCCCCACCCCACGCTGCTCCCAAAAGTGCACGCTACTTTGCTAA605'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNALegionella Pneumophila (Lp 120292)Whole cellIdentify an aptamer against Lp and utilise it as a biorecognition element in a biosensor to detect Lp in real-time and in situ.R10C1 shows significantly more binding to Lp than to Pseudomonas.10Flow Cytometry135 nMBiosensorWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/APatent application no US 16/850,355
ABdb_1386 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF501TAGGGAAGAGAAGGACATATGAT-TTTCTCAACGGGACCATCACTTACCTCAAGTACTTGGACG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1387 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF502TAGGGAAGAGAAGGACATATGAT-CCGGCTATCTCCCTACCGTGGCCGAGTACCTCAAACGTTT-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1388 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF503TAGGGAAGAGAAGGACATATGAT-GTCAACTCATTTATGGTGCTCCTCGTACCTCAGGTGGTTA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1389 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF504TAGGGAAGAGAAGGACATATGAT-GGCCATACCTCGTGCCTTCTGTGATCATCTCTATCAATTG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1390 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF505TAGGGAAGAGAAGGACATATGAT-CCTCTCTCTTACTGCTACTGGGCAGGGTACTCAATTACGT-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1391 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF506TAGGGAAGAGAAGGACATATGAT-CGGTCCCGACTCAATATTGTTCCCTCCCCTTATCAGGCGG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1392 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF507TAGGGAAGAGAAGGACATATGAT-TCCTCTAATCAACTCTATGCCTTATCCCCTTGGTCAGGAC-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1393 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF508TAGGGAAGAGAAGGACATATGAT-ACTGGCCTTGACACCCTGTTGTGGCTTGATGACAATAACA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.EF508 exhibited more than 20- to 800-fold higher binding to E. faecalis target cells than to non-target cells.11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)37 ± 4 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1394 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF509TAGGGAAGAGAAGGACATATGAT-CCTCACTCTTGACCCAAAGTGCATGCTCTATTCATTCGGA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1395 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF510TAGGGAAGAGAAGGACATATGAT-GCTTCTGTGCACATTAAGGCACTCGTCTTCACTGTGGTTC-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1396 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF511TAGGGAAGAGAAGGACATATGAT-CCTAACTCACTTACCAGCACGAGGTGCCTGTACCATCAAT-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1397 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF512TAGGGAAGAGAAGGACATATGAT-CTCTCATCACAGGAATTTGAATTTCCCTTGTGGACAGTAA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1398 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF513TAGGGAAGAGAAGGACATATGAT-GATGTGAATTCCGTCCCTTGGTCAGACACTTCAACACCGG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1399 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF514TAGGGAAGAGAAGGACATATGAT-TCTCGACGCTATGATCAAGACGCAGTATGATGGCACATCA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1400 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF515TAGGGAAGAGAAGGACATATGAT-TTAACCCTCATTTAATGGCCGCGTCAATCCGCAAAGGGTC-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1401 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF516TAGGGAAGAGAAGGACATATGAT-TTCCTTCGCAGGACACCGATGGCCAGGCGCGAGTCAATAT-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1404 326859852020Electrochemical aptasensor using boron-carbon nanorods decorated by nickel nanoparticles for detection of E. coli O157:H7Anti-E. coli O157:H7 aptamerATCCAGAGTGACGCAGCA-GGGTGGCGAGACTGGGCGGGTGTCGGGAAGTGAACCGGTGGCGTG-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDeveloped a label-free impedimetric aptasensor for the detection of E. coli O157:H7 employing boron-carbon nanorods decorated by nickel nanoparticles (BC-Ni) nanostructured platform.Detect E. coli O157:H7 selectively with a detection limit of 10 cfu and a dynamic detection range of 10(0) to 10(5) cfu in water, juice, and faecal samples.15Bio-Layer Interferometry (BLI)69.73 nMBiosensorMicrotiter Plate-based Cell SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1405 328107752020Highly adaptable and sensitive FRET-based aptamer assay for the detection of Salmonella paratyphi ASal 1ATTAGTCAAGAGGTAGACGCACATAAGGGGTCTGGTGTCGGGCCGCGGGTCAGGGGGGTAAGGGATTCTGGTCGTCGTGACTCCTAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Paratyphi A (ATCC 9150)Whole cellIdentify an aptamer and develop a GO/CdTeQDaptamer-based FRET assay for the detection of Salmonella paratyphi A.The detection limit was 10 CFU/mL within 15 min with no cross-reactivity with other bacterial species.11Indirect ELASA (aptamer linked immunosorbent assay)N/ABiosensorWhole Cell-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_1406 328107752020Highly adaptable and sensitive FRET-based aptamer assay for the detection of Salmonella paratyphi ASal 2ATTAGTCAAGAGGTAGACGCACATAAGGAGTCACGACGACCAGAAACGTTTGCGGTGTTGAGCGGTTCTGGTCGTCGTGACTCCTAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Paratyphi A (ATCC 9150)Whole cellIdentify an aptamer and develop a GO/CdTeQDaptamer-based FRET assay for the detection of Salmonella paratyphi A.The detection limit was 10(3) CFU/mL.11Indirect ELASA (aptamer linked immunosorbent assay)N/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1407 328107752020Highly adaptable and sensitive FRET-based aptamer assay for the detection of Salmonella paratyphi ASal 3ATTAGTCAAGAGGTAGACGCACATAACGGCGGCAGCGAGGGCGAACCAGGGGGGGCACACCGAGCTTCTGGTCGTCGTGACTCCTAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Paratyphi A (ATCC 9150)Whole cellIdentify an aptamer and develop a GO/CdTeQDaptamer-based FRET assay for the detection of Salmonella paratyphi A.N/A11Indirect ELASA (aptamer linked immunosorbent assay)N/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1408 328107752020Highly adaptable and sensitive FRET-based aptamer assay for the detection of Salmonella paratyphi ASal 4ATTAGTCAAGAGGTAGACGCACATAAGTATTTAGCGAACTCGCGGAGGTTCAGTAAAGAATGTACTTCTGGTCGTCGTGACTCCTAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Paratyphi A (ATCC 9150)Whole cellIdentify an aptamer and develop a GO/CdTeQDaptamer-based FRET assay for the detection of Salmonella paratyphi A.N/A11Indirect ELASA (aptamer linked immunosorbent assay)N/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1409 328107752020Highly adaptable and sensitive FRET-based aptamer assay for the detection of Salmonella paratyphi ASal 5ATTAGTCAAGAGGTAGACGCACATAGCCACTCGACCCGCCAGAAACGGCGACAGGATGGCCGCGGTTCTGGTCGTCGTGACTCCTAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Paratyphi A (ATCC 9150)Whole cellIdentify an aptamer and develop a GO/CdTeQDaptamer-based FRET assay for the detection of Salmonella paratyphi A.N/A11Indirect ELASA (aptamer linked immunosorbent assay)N/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1415 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV8AGTATACGTATTACCTGCAGC-CAATCATGACCGCCCACCTCACTCG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cell (Cell wall protein)Isolate aptamers that specifically bind V. vulnificus across all culture phases.The LOD of the V8 from cytometry is 29.96 CFU/mL, and the linear range is 102–5 × 105 CFU/mL.13Flow Cytometry11.22 ± 1.30 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AV8 and V13 can tolerate diluted serum as well as oyster infusion.Best CandidateN/AN/A
ABdb_1416 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV9AGTATACGTATTACCTGCAGC-CCTGGACATCATTGAGTACTCGTCT-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1417 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV11AGTATACGTATTACCTGCAGC-TCCCAACCAATACCAGTACGTTGTA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1418 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV12AGTATACGTATTACCTGCAGC-TATGGATTTGCGTCATGTTTATGTG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1419 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV13AGTATACGTATTACCTGCAGC-CCAACCCTATGCTTCAACGGTCTTT-GCAAAGATCTCCGAGATATCG675'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow Cytometry15.47 ± 0.39 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AV8 and V13 can tolerate diluted serum as well as oyster infusion.Best CandidateN/AN/A
ABdb_1420 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV18AGTATACGTATTACCTGCAGC-TGTGGGTGGGTGGGTGGTATCTGCA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1421 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV20AGTATACGTATTACCTGCAGC-CATCCCCTCTCCTGTTGCCCTGACA-GCAAAGATCTCCGAGATATCG675'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1422 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV28AGTATACGTATTACCTGCAGC-CCTGGACATCATTGAGTACTCGTCT-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1423 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV31AGTATACGTATTACCTGCAGC-TGTGGGTGGGATTAGGTTCGGGTGG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1424 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV38AGTATACGTATTACCTGCAGC-CCAGACTTCAATCGCGTCAACCGTT-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1425 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV39AGTATACGTATTACCTGCAGC-TGATGGTTGTATGACTGGATGTCAA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1426 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV40AGTATACGTATTACCTGCAGC-TCCCCTTTGCATGGCGGTGACACTG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1427 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV41AGTATACGTATTACCTGCAGC-CACCTAGAACACATTGCAACATTAG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1428 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV44AGTATACGTATTACCTGCAGC-TGCTCCTCGACTGTTGTTAATCGTG-GCAGATCTCCGAGATATCG655'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1429 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV49AGTATACGTATTACCTGCAGC-TGACATCGTCTGACCTCCACAAGCA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1430 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV53AGTATACGTATTACCTGCAGC-TGGGTCCGTATGTTGGTGTATGTGA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1431 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV59AGTATACGTATTACCTGCAGC-TGTATACCCGACCGTACCGACGTAA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1432 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV69AGTATACGTATTACCTGCAGC-TCACCTTCACACACTCCCTTCTTCG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1433 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV71AGTATACGTATTACCTGCAGC-CCTGTACAAGCAGTATGTCAGCTGA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1434 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyTV8CAATCATGACCGCCCACCTCACTCG255'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.The fluorescent intensity of V. vulnificus was significantly greater than that of other species.13Flow Cytometry17.44 ± 1.30 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1435 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyTV13CCAACCCTATGCTTCAACGGTCTTT255'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.The fluorescent intensity of V. vulnificus was significantly greater than that of other species.13Flow Cytometry13.21 ± 2.19 nmBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1455 325158422020The Diversity of a Polyclonal FluCell‐SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosaC1R1TAGGGAAGAGAAGGACATATGAT-GCGCGCGAGATTAACCCCCCAATGCTGCACCGAGCCACGA-TCAAGTGGTCATGTACTAGTCAA865'-TAGGGAAGAGAAGGACATATGAT-40N-TCAAGTGGTCATGTACTAGTCAA-3'ssDNAPseudomonas Aeruginosa PAO1Outer membrane protein (OMP) OprF, OprM and OprDIdentify an aptamer library that specifically targets different clinically relevant strains, thereby inhibiting virulence-associated cellular functions.With 250 cells as the lowest measured cell number by the C1R1. The R16 aptamer library outperformed single aptamers in labelling and inhibiting biofilm and motility.16Fluorescence Spectroscopy31 ± 2 nMTherapeuticsFluCell‐SELEX5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1456 325158422020The Diversity of a Polyclonal FluCell‐SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosaC2R1TAGGGAAGAGAAGGACATATGAT-GCGGCAGGGAAGGACTATGTGGGTGAAAGGAGTGCGCGGG-TCAAGTGGTCATGTACTAGTCAA865'-TAGGGAAGAGAAGGACATATGAT-40N-TCAAGTGGTCATGTACTAGTCAA-3'ssDNAPseudomonas Aeruginosa PAO1Outer membrane protein (OMP) OprF, OprM and OprDIdentify an aptamer library that specifically targets different clinically relevant strains, thereby inhibiting virulence-associated cellular functions.The R16 aptamer library outperformed single aptamers in labelling and inhibiting biofilm and motility.16Fluorescence SpectroscopyN/ATherapeuticsFluCell‐SELEX5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1457 325158422020The Diversity of a Polyclonal FluCell‐SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosaC2R2TAGGGAAGAGAAGGACATATGAT-GCAGCGGGATGGGGTAAATGGTGGCGAGAGGCGTCGGGGG-TCAAGTGGTCATGTACTAGTCAA865'-TAGGGAAGAGAAGGACATATGAT-40N-TCAAGTGGTCATGTACTAGTCAA-3'ssDNAPseudomonas Aeruginosa PAO1Outer membrane protein (OMP) OprF, OprM and OprDIdentify an aptamer library that specifically targets different clinically relevant strains, thereby inhibiting virulence-associated cellular functions.The R16 aptamer library outperformed single aptamers in labelling and inhibiting biofilm and motility.16Fluorescence SpectroscopyN/ATherapeuticsFluCell‐SELEX5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1458 325158422020The Diversity of a Polyclonal FluCell‐SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosaC4R2TAGGGAAGAGAAGGACATATGAT-GCGGGTTGACTAGTACATGACCACTTGAGTCGCTTGAACT-TCAAGTGGTCATGTACTAGTCAA865'-TAGGGAAGAGAAGGACATATGAT-40N-TCAAGTGGTCATGTACTAGTCAA-3'ssDNAPseudomonas Aeruginosa PAO1Outer membrane protein (OMP) OprF, OprM and OprDIdentify an aptamer library that specifically targets different clinically relevant strains, thereby inhibiting virulence-associated cellular functions.Labelling efficiency by C4R2 of approximately 60 % fluorescence signal relative to R16 values. The R16 aptamer library outperformed single aptamers in labelling and inhibiting biofilm and motility.16Fluorescence SpectroscopyN/ATherapeuticsFluCell‐SELEX5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1459 325158422020The Diversity of a Polyclonal FluCell‐SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosaC6R3TAGGGAAGAGAAGGACATATGAT-GCGGGGAGAGGCGAAAGAAGCTGGGATGGAAGGGCGTAGG-TCAAGTGGTCATGTACTAGTCAA865'-TAGGGAAGAGAAGGACATATGAT-40N-TCAAGTGGTCATGTACTAGTCAA-3'ssDNAPseudomonas Aeruginosa PAO1Outer membrane protein (OMP) OprF, OprM and OprDIdentify an aptamer library that specifically targets different clinically relevant strains, thereby inhibiting virulence-associated cellular functions.The R16 aptamer library outperformed single aptamers in labelling and inhibiting biofilm and motility.16Fluorescence SpectroscopyN/ATherapeuticsFluCell‐SELEX5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1460 325158422020The Diversity of a Polyclonal FluCell‐SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosaC10R5TAGGGAAGAGAAGGACATATGAT-GCAGCCACAGCAGAGACGGGAAGGGCCAGGGTTGAGCGGG-TCAAGTGGTCATGTACTAGTCAA865'-TAGGGAAGAGAAGGACATATGAT-40N-TCAAGTGGTCATGTACTAGTCAA-3'ssDNAPseudomonas Aeruginosa PAO1Outer membrane protein (OMP) OprF, OprM and OprDIdentify an aptamer library that specifically targets different clinically relevant strains, thereby inhibiting virulence-associated cellular functions.The R16 aptamer library outperformed single aptamers in labelling and inhibiting biofilm and motility.16Fluorescence SpectroscopyN/ATherapeuticsFluCell‐SELEX5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1461 325158422020The Diversity of a Polyclonal FluCell‐SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosaC10R6TAGGGAAGAGAAGGACATATGAT-GCGGCGGTGGGGCTTTCGGTGATTTGGGCGGTTTGGCGGG-TCAAGTGGTCATGTACTAGTCAA865'-TAGGGAAGAGAAGGACATATGAT-40N-TCAAGTGGTCATGTACTAGTCAA-3'ssDNAPseudomonas Aeruginosa PAO1Outer membrane protein (OMP) OprF, OprM and OprDIdentify an aptamer library that specifically targets different clinically relevant strains, thereby inhibiting virulence-associated cellular functions.The R16 aptamer library outperformed single aptamers in labelling and inhibiting biofilm and motility.16Fluorescence SpectroscopyN/ATherapeuticsFluCell‐SELEX5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1462 326630062020Selection of Specific DNA Aptamers for Hetero-Sandwich-Based Colorimetric Determination of Campylobacter jejuni in FoodCJA1CTGCGATCAAGTTACGCACCTCGCCATGTTCCCCGCCCGGCATGTGTTATGCCCCTGTG595'-AGCAGCACAGAGGTCAGATG-N59-TTCACGGTAGCACGCATAGG-3'ssDNACampylobacter JejuniWhole cellIdentify aptamers and develop a hetero-sandwich-based assay for colorimetric analysis of C. jejuni from contaminated samples.C. jejuni can be detected from 1.7 × 10(1) to 1.7 × 10(6) CFU/mL. The limit of detection (LOD) is obtained as 10 CFU/mL in PBS and 13 CFU/mL in contaminated milk samples.12Fluorescence Binding Assay1.37 ± 0.28 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1463 326630062020Selection of Specific DNA Aptamers for Hetero-Sandwich-Based Colorimetric Determination of Campylobacter jejuni in FoodCJA2GTTGGGTGCGGCGGGAGGGTCCCATCGGAAACCAAGCTGTGCTGAGATTTTTCCGCGAT595'-AGCAGCACAGAGGTCAGATG-N59-TTCACGGTAGCACGCATAGG-3'ssDNACampylobacter JejuniWhole cellIdentify aptamers and develop a hetero-sandwich-based assay for colorimetric analysis of C. jejuni from contaminated samples.N/A12Fluorescence Binding Assay1.78 ± 0.88 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1464 326630062020Selection of Specific DNA Aptamers for Hetero-Sandwich-Based Colorimetric Determination of Campylobacter jejuni in FoodCJA3CACGCAAGTTAATCAGCGTGACATCAGGTCTAGATTCCGGCTTATAGCGCCAGGTTTCC595'-AGCAGCACAGAGGTCAGATG-N59-TTCACGGTAGCACGCATAGG-3'ssDNACampylobacter JejuniWhole cellIdentify aptamers and develop a hetero-sandwich-based assay for colorimetric analysis of C. jejuni from contaminated samples.N/A12Fluorescence Binding Assay2.01 ± 0.90 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1465 326630062020Selection of Specific DNA Aptamers for Hetero-Sandwich-Based Colorimetric Determination of Campylobacter jejuni in FoodCJA4GGTGGGCTCTGTTCGGGAGGGGTATTGTTAGGACCGAGAGGCTGCATTCCGCCAGCTGC595'-AGCAGCACAGAGGTCAGATG-N59-TTCACGGTAGCACGCATAGG-3'ssDNACampylobacter JejuniWhole cellIdentify aptamers and develop a hetero-sandwich-based assay for colorimetric analysis of C. jejuni from contaminated samples.N/A12Fluorescence Binding Assay2.26 ± 0.91 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1466 326630062020Selection of Specific DNA Aptamers for Hetero-Sandwich-Based Colorimetric Determination of Campylobacter jejuni in FoodCJA5GCTCTGTCTTTTCAGCTTTATCCGGGTGTGGTGGGGGGGGGATTTGCTTGGTCGGTTCT595'-AGCAGCACAGAGGTCAGATG-N59-TTCACGGTAGCACGCATAGG-3'ssDNACampylobacter JejuniWhole cellIdentify aptamers and develop a hetero-sandwich-based assay for colorimetric analysis of C. jejuni from contaminated samples.N/A12Fluorescence Binding Assay3.53 ± 1.38 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1467 329801072020A novel method combining aptamer-Ag10NPs based microfluidic biochip with bright field imaging for detection of KPC-2-expressing bacteriaXK10GGCAGGACACCGTAACGGGTATGCAGCTATCCCGGGCGCTGTCTGAAGATCGTGTGCTGCT61N/AssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseDeveloped a PDMS/glass microfluidic biochip integrated with aptamer-modified Ag(10)NPs nano-biosensors to detect whether bacteria express KPC-2.Detects the target bacterium with a detection limit of 10(2) CFU and a capture efficiency exceeding 90% in ∼1 h.8Surface Plasmon Resonance (SPR)0.81 nMBiosensorProtein SELEX and Whole Cell-SELEX5'-Biotinylated and 5′-Thiolated (SH-AAAAA) and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1470 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx1.1)ATCCAGAGTGACGCAGCA-GTAGTTTGTTGGTTATTACGGCGGGTTGCGATGGGTGCGAATCGG-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx1 (Stx1.1)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.LOD of 44.5 pg/mL for stx1 with minimal cross-reactivity and a dynamic response range from 50 pg/mL to 100 ng/mL.7Bio-Layer Interferometry (BLI)8.27 nM (of peptide) and 47.2 pM (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1471 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx1.3)ATCCAGAGTGACGCAGCA-GGATAGGACGTCAAATTAGGGCCCGGTACAACGAAAGCCCACAAC-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx1 (Stx1.2)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.N/A7Bio-Layer Interferometry (BLI)31.0 nM (of peptide) and 30.3 μΜ (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1472 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx2.1)ATCCAGAGTGACGCAGCA-GGGGGCAGGTTCATGGCTTGGGTGCGGTGGGCATGATTCGTGGTG-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx2 (Stx2.1)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.N/A7Bio-Layer Interferometry (BLI)45.2 nM (of peptide) and 83.8 nM (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1473 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx2.2)ATCCAGAGTGACGCAGCA-TGTATCTCTTACTTAAGCCTTTGGTTCGGTAACAGCCTGGCATGC-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx2 (Stx2.2)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.N/A7Bio-Layer Interferometry (BLI)110 nM (of peptide) and 25.2 μM (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1474 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx2.3)ATCCAGAGTGACGCAGCA-GGAAAGGACGTCAAATTAGGGGCCGGGACAACGAAAGCCCACAAC-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx2 (Stx2.3)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.LOD of 41.3 pg/mL for stx2 with minimal cross-reactivity and a dynamic response range from 50 pg/mL to 100 ng/mL.7Bio-Layer Interferometry (BLI)4.6 nM (of peptide) and 28.6 pM (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1476 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp1N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry18.10 ± 6.2 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1477 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp2N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry13.38 ± 3.8 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1478 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp3N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry11.46 ± 4.1 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1479 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp4N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry14.18 ± 4.3 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1480 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp5N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry21.78 ± 9.2 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1481 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp6TAGGGAAGAGAAGGACATATGAT-GCGCTGTGCGGATGTCATGATGTGCCTCTTCCCTGTGTCCGC-TTGACTAGTACATGACCACTTGA885'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.The detection limit of this aptasensor was as low as 10(5) CFU/ml within 10 minutes and a linear range of 10(5)-10(8) CFU/ml.10Flow Cytometry9.82 ± 3.6 nMBiosensorWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/ASubmission Number Petty patent no.: 1803001734
ABdb_1482 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp7N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry12.85 ± 4.1 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1483 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp8N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry32.90 ± 17.4 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1484 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp9N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry12.18 ± 4.3 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1485 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp10N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry32.85 ± 12.2 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1490 355161862020Selection of potential aptamers for specific growth stage detection of Yersinia enterocoliticaM1AGCAGCACAGAGGTCAGATGATATAACCTTAATAAATAAAATATAAATTATTTAATCTTACCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-40N-TTCACGGTAGCACGCATAGG-3'ssDNAYersinia Enterocolitica (CICC 21669)Whole cellIdentified aptamers bound to the different growth stages of Y. enterocolitica.Showed good affinity to all different stages of bacteria (adjustment phase, log phase, and pre-stationary phase).10Flow Cytometry37.93 ± 7.88 nMDetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1491 355161862020Selection of potential aptamers for specific growth stage detection of Yersinia enterocoliticaM5AGCAGCACAGAGGTCAGATGCTTGGTTCCACCGTACTGACTGTAGTAAAATCTGATCACTCCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-40N-TTCACGGTAGCACGCATAGG-3'ssDNAYersinia Enterocolitica (CICC 21669)Whole cellIdentified aptamers bound to the different growth stages of Y. enterocolitica.Showed good affinity to all different stages of bacteria (adjustment phase, log phase, and pre-stationary phase).10Flow Cytometry74.96 ± 21.34 nMDetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1492 355161862020Selection of potential aptamers for specific growth stage detection of Yersinia enterocoliticaM7AGCAGCACAGAGGTCAGATGTAGTCGGTCTTCTTGTTTGAAACTGCTAATTTTGAAAAAACCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-40N-TTCACGGTAGCACGCATAGG-3'ssDNAYersinia Enterocolitica (CICC 21669)Whole cellIdentified aptamers bound to the different growth stages of Y. enterocolitica.Showed good affinity to all different stages of bacteria (adjustment phase, log phase, and pre-stationary phase).10Flow Cytometry73.02 ± 18.76 nMDetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1494 329948962020Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1V.ch27GCCTGTTGTGAGCCTCCTAAC-GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCAC-CATGCTTATTCTTGTCTCC815'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAVibrio Cholerae O1 [Inaba (ATCC 39315) and Ogawa]Whole cellIdentify aptamers and develop aptamer-nanobody-based ELISA for V. cholerae O1 detection.The binding efficiency for V.ch27 was 53.3%.12Flow Cytometry20.186 ± 3.655 pMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1495 329948962020Aptamer-nanobody based ELASA for detection of Vibrio cholerae O2V.ch47GCCTGTTGTGAGCCTCCTAAC-CGTATTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTC-CATGCTTATTCTTGTCTCC795'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAVibrio Cholerae O1 [Inaba (ATCC 39315) and Ogawa]Whole cellIdentify aptamers and develop aptamer-nanobody-based ELISA for V. cholerae O1 detection.Identify 10(4) CFU/ml with 25 pM of biotinylated aptamer and only 20 μg/ml of VHH without any cross-reactivity.12Flow Cytometry15.404 ± 4.776 pMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1504 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-10 (truncated)GGCAGGACACCGTAACGGGTATGCAGCTATCCCGGGCGCTGTCTGAAGATCGTGTGCTGCT615'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.The detection limit of Biotin-XK-10 was 104 CFU/mL, and that of Biotin-Ag-XK-10 was 103 CFU/mL.4Surface Plasmon Resonance (SPR) and Microarray Chip0.81 ± 0.13 nM (by SPR) and 12.9 ± 1.03 nM (by microarray chip)BiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/ABest CandidateN/AN/A
ABdb_1505 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-1ACCCCATATCGCGTCAAATTTGGCTACCTCACATGCCCAC405'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1506 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-2GACAGGCAGGACACCGTAAC-AAGCCCGTGCGGCTTCGTGCGATGGATACAGTGGGCGGGG-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1507 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-3GACAGGCAGGACACCGTAAC-ACTAGTAACCCCTAACATCTTCCGCGTTCTTATAGGCCCC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1508 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-4GACAGGCAGGACACCGTAAC-ACTCCATGGGGTTTGTAGGGCTCGCTGTAATTATACTTTA-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1509 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-5GACAGGCAGGACACCGTAAC-ACTCCCCCTTCCCTGTCTGCTCTACCTGCCTGTTCCCTGC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1510 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-6GACAGGCAGGACACCGTAAC-AGGTTGTTGCCGCTCCCCTCGTCTGTTGGGGGTTGTCCTC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1511 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-7GACAGGCAGGACACCGTAAC-ATAACTTCTCTCCCGTCAGTCTCCGCAGCCCCATTCCATC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1512 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-8GACAGGCAGGACACCGTAAC-ATCGCAGGGCCCCCGTTATTATTCCCCACTTCCTTCTGTC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1513 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-9GACAGGCAGGACACCGTAAC-CGGGCTTATCGGTCCGTCTGGAAGGTCCTTTCCTATTCGC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1514 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-10GACAGGCAGGACACCGTAAC-GGGTATGCAGCTATCCCGGGCGCTGTCTGAAGATCGTGTG-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1515 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-11GACAGGCAGGACACCGTAAC-GGGTATACAGCTATCCCGGGCGCTGTCTGAAGATCGTGTG-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1516 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-12GACAGGCAGGACACCGTAAC-GGGTATGCAGCTATCCCGGGCGCTGTCTGAAGATCGTGTC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1517 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-13GACAGGCAGGACACCGTAAC-GGGTACGCAGCTATCCCGGGCGCTGTCTGAAGATCGTGTG-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1518 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-14GACAGGCAGGACACCGTAAC-GTACTGCCTCCCTCCTGCCGTCCCTCTCTCTAGTCTCTGC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1519 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-15GACAGGCAGGACACCGTAAC-GTCCGCCTCTGTATTCCTGCCTCTGTTCCTGCCTTCTGTC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1520 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-16GACAGGCAGGACACCGTAAC-TATACCTTCCATCCCGGTCCTGCTCCTCGTCTTTTGTGTC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1521 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-17GACAGGCAGGACACCGTAAC-TCCCACCTCTTCCCCCCTTCTAGGTTCATCTCGCGCCACC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1522 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-18GACAGGCAGGACACCGTAAC-TCGTCATTCGTCATTCCTGCCCTTTCCTGCCTGATCTGTC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1523 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-19GACAGGCAGGACACCGTAAC-TGCCCTCCCTTCCTGCCTGGTCCTGCCGTATTATTTTGTC-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1524 333790052021Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEXXK-20GACAGGCAGGACACCGTAAC-TTTCCTGTATTGTGGAAGTAAACGTCTGAGTGGTCGATGT-CTGCTACCTCCCTCCTCTTC805'-GACAGGCAGGACACCGTAAC-N40-CTGCTACCTCCCTCCTCTTC-3' (Library I) and 5'-GACAGGCAGGACACCGTAACNNNGTCCNNNNNGGACNNGCCNNNNGGCNNNGTTACGGTGCTGCTACCTCCCTCCTCTTC-3' (Library II)ssDNAEscherichia Coli expressing KPC-2 (KPC-2 E. Coli)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseIdentify aptamers that specifically recognize KPC-2 and KPC-2 E. coli.N/A4Surface Plasmon Resonance (SPR) and Microarray ChipN/ABiosensorPrecision-SELEX (Protein SELEX and Whole Cell-SELEX)Biotinylated or FITC or TAMRA LabeledN/AN/AN/AN/AN/A
ABdb_1525 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-2AAGGAGCAGCGTGGAGGTTA-CCAGGAGGACCCTATTCTCGTGTATCGACGAGATCCAGTG-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.Limit of detection (LOD) of HPA-2 was 88 cfu/mL.9Fluorescence Spectroscopy19.3 ± 3.2 nMBiosensorSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1526 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-1AAGGAGCAGCGTGGAGGTTA-CGCCTGATCCAGGTGCTATCTTCCGCCCTGTTTCTTTGGT-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1527 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-3AAGGAGCAGCGTGGAGGTTA-CTCCTCGGTCCTTTCAGGTAGACGCTCTTACGCCCTCAGT-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.The binding ability order was HPA-2 > HPA-3 > HPA-5 > HPA-9.9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1528 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-4AAGGAGCAGCGTGGAGGTTA-CGTGTATCCCCTGTGTGTTTGTACTCGGCTACTGTATCCG-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1529 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-5AAGGAGCAGCGTGGAGGTTA-CTCGTTTGGATACGTCATCGGTAGGACTACGGTACCTAAAC-ACCACGACGACACACCCTAA815'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.The binding ability order was HPA-2 > HPA-3 > HPA-5 > HPA-9.9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1530 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-6AAGGAGCAGCGTGGAGGTTA-GCACAGGAGACAGGGGGAGATGATACTCGGCGTTTCAAGG-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1531 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-7AAGGAGCAGCGTGGAGGTTA-CTCGCGCCCTTCTTTCAGTAGCAGTGTACGGATCTTGCGG-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1532 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-8AAGGAGCAGCGTGGAGGTTA-TGCATATCGCTAGCTGATAAGCTGATGCCATCGTGTCTAC-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1533 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-9AAGGAGCAGCGTGGAGGTTA-GCATCTTTGAGGAATTTTCGTCAAAGGACCGAGTAACGGC-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.The binding ability order was HPA-2 > HPA-3 > HPA-5 > HPA-9.9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1534 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-10AAGGAGCAGCGTGGAGGTTA-AGTTGCTCTTGATCGTCGACCAGTCGCTATGCAGCACCCC-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1535 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-11AAGGAGCAGCGTGGAGGTTA-CTGCACGAATGATCCCCCGCCATGCTGTAGTTCCGTCTTA-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1536 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-12AAGGAGCAGCGTGGAGGTTA-CTGTAACGCCCCGCTGATTCTTTCTCAGCGTGCTAGGCGG-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1537 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-13AAGGAGCAGCGTGGAGGTTA-ATCGCTCCGTCCAGTAAGAGTTGCCTACAATGCCCGCCGGC-ACCACGACGACACACCCTAA815'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1538 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-14AAGGAGCAGCGTGGAGGTTA-CAAATGAGCCTTAGAGCTGTGCAGAGTTCGAAGCTTGGTG-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1539 335857562021Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA AptamersHPA-15AAGGAGCAGCGTGGAGGTTA-CCTCGGTGTTCGTTCTGACTACGTTCCCTGGGACCCGCGT-ACCACGACGACACACCCTAA805'-AAGGAGCAGCGTGGAGGTTA-N40-ACCACGACGACACACCCTAA-3'ssDNAHelicobacter Pylori (ATCC 43504)Whole cellIdentify aptamers that can detect H. pylori with no specificity for other bacteria.N/A9Fluorescence SpectroscopyN/ABiosensorSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1570 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI05TAGCTCACTCATTAGGCACGACTCACTCTTGAAGGGGTGAGGCATGGTGGTATCAGCATAGTTAAGCCAGCC725'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry144.4 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1571 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI08TAGCTCACTCATTAGGCACATGCACCGAGGTCAGAAGTGCCTGTAATACACACCCCTCGGCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry301.4 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1572 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI 9TAGCTCACTCATTAGGCACCGAGTGCAAGATGTCCTACCCGATGAAGTAGGTTGGGTCTGCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry279.7 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1573 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI23TAGCTCACTCATTAGGCACCTTGGCTTAAAATGATAGCTAGTGGCAGATTGTTAATTTGGCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.The assay is able to detect 10(2) CFU/mL cells.10Flow Cytometry231.2 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1574 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI27TAGCTCACTCATTAGGCACATGGCAAGGTTGCCTTTTTGAGCGCGCTGCATAGTTAAGCCAGCC645'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry328.9 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1575 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI31TAGCTCACTCATTAGGCACCAGCCGGAAGGACCAGCTAGCTCACTCATTAGGCACCGAAGTGTGGTGCATAGTTAAGCCAGCC835'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry186.1 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1576 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI37TAGCTCACTCATTAGGCACGAACGGGCTTGTCTTTCCATAATTCGTGCGAAAGTGCCGCATAGTTAAGCCAGCC745'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry176.7 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1577 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI42TAGCTCACTCATTAGGCACTAGCGGAAGCTTAGTGTAAACGAGTGATAATGTGTTATGTGCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry173.6 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1599 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE1TAGGGAAGAGAAGGACATATGA-CGATTACCGGAGCTGTGTCACCGGGCGCCAGTTGATGTGG-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1600 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE2TAGGGAAGAGAAGGACATATGA-TATGATGGGAGTAACGATTGTCCGACATGGTACCACCCCA-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1601 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE3TAGGGAAGAGAAGGACATATGA-GGTGCACCTCTCGCCCGAATCAGATCCCCACGGCGCCCCCTA-TTGACTAGTACATGACCACTTGA875'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1602 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE4TAGGGAAGAGAAGGACATATGA-ATCGGCTACGAGGTCCCGACTGCCGGACTGGCCCTTGGTC-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1603 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE5TAGGGAAGAGAAGGACATATGA-ACACCGCCACGATGCAACTGCCAAACGCACCGTACGCCTG-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1604 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE6TAGGGAAGAGAAGGACATATGA-CGAGAGGCCGCTGGGTCTAGACGTCCGGGTGCCACTCGCG-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1605 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE7TAGGGAAGAGAAGGACATATGA-TAACGCCTGTAGTACTCCACCTTAATCCGTTGCCAGGAAT-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1606 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE8TAGGGAAGAGAAGGACATATGA-CATTCTGCACCGCCATCAGTCTTTCTTCGATACCGCGTTT-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.The limit of detection observed visually was 10(2) cells/mL with GO coating and 10(3) cells/mL without GO. The detection limit in real-time coconut water samples was also 10(2) cells/mL.7Fluorescence Microplate Reader (Fluorescence Spectroscopy)15.90 ± 3.07 nMBiosensorWhole Cell-SELEX5'-FAM Labeled, 5'-Thiolated and 5'-Amidation (NH₂)N/AN/ABest CandidateN/AN/A
ABdb_1607 358467532022Selection and Identification of a DNA Aptamer for Multidrug-Resistant Acinetobacter baumannii Using an In-House Cell-SELEX MethodologyA01CAGGGGACGCACCAAGG-TTTTGTTTTTTCTTTGCTTCTTTTTGCTTTTTTTT-CCATGACCCGCGTGCTGCGTGA745'-CAGGGGACGCACCAAGG-N35-CCATGACCCGCGTGCTGCGTGAT-3'ssDNAMultidrug-resistant (MDR) Acinetobacter BaumanniiWhole cellIdentify aptamers against MDR A. baumannii.Preferential binding to A. baumannii over other species. 25% of positive events for A. baumannii above the 12.66% of the negative control.7Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledAptamer binding does not seem to interfere with the A. baumannii cell growth even after 24h (5.88E+02 x 1.45E+03; p=0.700).N/AN/AN/AN/A
ABdb_1608 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su12GGGAGUCGACCGACCAGAA-CAUACUGAGUAAGAUCGGAAAUUUCGGUGUAAGGCCACGG-UAUGUGCGUCUACAUCUAGACUCAU845'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.Significantly reduced biofilm formation by 61.2%.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/ABest CandidateN/AN/A
ABdb_1609 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su057GGGAGUCGACCGACCAGAA-UGGAUGUAUGGAACUUGCAGAUCUUAACUGCACGAAGCGU-UAUGUGCGUCUACAUCUAGACUCAU845'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.N/A8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_1610 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su15GGGAGUCGACCGACCAGAA-ACACGUUGCUGAAACAUACCGAGUAACAUAAAGCGGGUG-UAUGUGCGUCUACAUCUAGACUCAU835'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.N/A8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_1612 356162772022Targeted inhibition of methicillin-resistant Staphylococcus aureus biofilm formation by a graphene oxide-loaded aptamer/berberine bifunctional complexAptamer 1GGGAGCTCAGAATAAACGCTCAATACTATCGCGGAGACAGCGCGGGAGGCACCGGGGAGATCCGGGCCTCATGTCGAA785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Penicillin binding protein 2a (PBP2a)Identify a PBP2a-targeted aptamer in conjunction with GO for the delivery of berberine to treat chronic infections caused by MRSA biofilms.The linear range was 10^3–10^7 CFU/mL, and the detection limit for the fluorescent assay was 800 CFU/mL. The inhibition rate peaked at 92.8% after treatment with the GO-berberine/aptamer 1 (containing 200 nM aptamer 1) complex.14Fluorescence Spectroscopy82.97 ± 8.86 nMDiagnostic/TherapeuticsSELEXFAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1613 356162772022Targeted inhibition of methicillin-resistant Staphylococcus aureus biofilm formation by a graphene oxide-loaded aptamer/berberine bifunctional complexAptamer 2GGGAGCTCAGAATAAACGCTCAAGGCACACAGGACTATACAGTGTTGCAGTGTTGCTGGATCCGGGCCTCATGTCGAA785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Penicillin binding protein 2a (PBP2a)Identify a PBP2a-targeted aptamer in conjunction with GO for the delivery of berberine to treat chronic infections caused by MRSA biofilms.N/A14Fluorescence Spectroscopy152.92 ± 29.26 nMDiagnostic/TherapeuticsSELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_1629 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt1ATCCAGAGTGACGCAGCA-TGACAGACGGGAGAGACACGACGGGGGCGGGGAGTGAGAAGCGCGCGCTG-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.Display a linear range between 7.0 × 10(4) and 7.0 × 10(7) CFU/mL, and the limit of detection of SWV was 7.0 × 10(4) CFU/mL.8Fluorescence Spectroscopy11 nMBiosensorWhole Cell-SELEXFAM Labeled and ThiolatedN/AN/ABest CandidateN/AN/A
ABdb_1630 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt2ATCCAGAGTGACGCAGCA-TGTGGTGTACGTATCATTTATGCTTTAATAGTGTGCCGTTGTTGCTTGCC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1631 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt3ATCCAGAGTGACGCAGCA-TGACGGGTAGGACTTCCTGTGTTAGGTTGTAATGTTGGTGAAGCGTCCCG-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1632 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt4ATCCAGAGTGACGCAGCA-TGGGGCCGGACAGGGCAAACTGGCTGAAGCGGAGGGTGTCGGCGCCGCTC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1633 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt5ATCCAGAGTGACGCAGCA-TGAGGGGAGGTAAGGGGTGAGCTCGCATGTACCGTTGGGAGAGGGGGGGC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1634 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt6ATCCAGAGTGACGCAGCA-TGTGGGCAAGTCGGTGACGAACCAGGTGTCAAGGTCCTGCGCGCTCGCAC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1635 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt7ATCCAGAGTGACGCAGCA-TGGAGCGGTGGGCACGGTTGGGTCGCGTGGATGGAGGCGGGCGTGTGCGC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1636 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt8ATCCAGAGTGACGCAGCA-TGGGACGGTGCGGGGGAGGAGGATGAGCGTGGTCGTTGGGTGGCTGCCGC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1637 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt9ATCCAGAGTGACGCAGCA-CGAACGTTAGACGATTTGTGCAGTAGTGGCCACAGCCTAATCGTGTTGCC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1638 360050122022Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocoliticaApt10ATCCAGAGTGACGCAGCA-TGCCAGTATGCCCACTCTCATTCGGCTTCTTTGTGTTGCATGTTACGCCC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAYersinia Enterocolitica (WA-314)Virulence factor Yersinia adhesin A (YadA)Identify an aptamer against YadA and develop an electrochemical biosensor for its detection.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1642 351046732022An antigen-targeting assay for Lyme disease: Combining aptamers and SERS to detect the OspA proteinIon-OspA-59CATGACACCGTACCTGCTCT-TGCTTTTCGTGCGCGCATAAAATACCTTGATACTGTGCCGTATGAAAGCG-AAGCACGCCAGGGACTATTA905'-CATGACACCGTACCTGCTCT-N50-AAGCACGCCAGGGACTATTA-3'ssDNABorrelia BurgdorferiOuter Surface Protein A (OspA)Develop SERS-based aptasensor to detect OspA in serum samples.OspA limit-of-detection determined to be 1 × 10−4 ng/mL with 91% sensitivity and 96% specificity.10Fluorescence anisotropy (FA)2.18 nMBiosensorRAPID-SELEX5'-DTPA dithiol and T at 86 position is replaced by 5-Octadiynyl dU moeityN/AN/ABest CandidateN/AN/A
ABdb_1643 351046732022An antigen-targeting assay for Lyme disease: Combining aptamers and SERS to detect the OspA proteinIon-OspA-21CATGACACCGTACCTGCTCT-AATAAGCACGCCAGGGACTATTAGATCGGAATAGCACACGTCTGAACTCC-AAGCACGCCAGGGACTATTA905'-CATGACACCGTACCTGCTCT-N50-AAGCACGCCAGGGACTATTA-3'ssDNABorrelia BurgdorferiOuter Surface Protein A (OspA)Develop SERS-based aptasensor to detect OspA in serum samples.N/A10Fluorescence anisotropy (FA)N/ABiosensorRAPID-SELEX5'-DTPA dithiol and T at 86 position is replaced by 5-Octadiynyl dU moeityN/AN/AN/AN/AN/A
ABdb_1644 351046732022An antigen-targeting assay for Lyme disease: Combining aptamers and SERS to detect the OspA proteinIon-OspA-46CATGACACCGTACCTGCTCT-AATAAGCACGCCAGGGACTATTAGATCGGAAGAGCACACGTGTGAACTCC-AAGCACGCCAGGGACTATTA905'-CATGACACCGTACCTGCTCT-N50-AAGCACGCCAGGGACTATTA-3'ssDNABorrelia BurgdorferiOuter Surface Protein A (OspA)Develop SERS-based aptasensor to detect OspA in serum samples.N/A10Fluorescence anisotropy (FA)N/ABiosensorRAPID-SELEX5'-DTPA dithiol and T at 86 position is replaced by 5-Octadiynyl dU moeityN/AN/AN/AN/AN/A
ABdb_1645 351046732022An antigen-targeting assay for Lyme disease: Combining aptamers and SERS to detect the OspA proteinIon-OspA-22CATGACACCGTACCTGCTCT-ACGAGATTCAAGCACTCCAGGGACTATTAGATCGGAAGAGCACACGTCTG-AAGCACGCCAGGGACTATTA905'-CATGACACCGTACCTGCTCT-N50-AAGCACGCCAGGGACTATTA-3'ssDNABorrelia BurgdorferiOuter Surface Protein A (OspA)Develop SERS-based aptasensor to detect OspA in serum samples.N/A10Fluorescence anisotropy (FA)N/ABiosensorRAPID-SELEX5'-DTPA dithiol and T at 86 position is replaced by 5-Octadiynyl dU moeityN/AN/AN/AN/AN/A
ABdb_1646 351046732022An antigen-targeting assay for Lyme disease: Combining aptamers and SERS to detect the OspA proteinIon-OspA-39CATGACACCGTACCTGCTCT-ACGAGATTCAAGCACGCCAGGGATTATTAGATCGGAAGAGCACACGTCTG-AAGCACGCCAGGGACTATTA905'-CATGACACCGTACCTGCTCT-N50-AAGCACGCCAGGGACTATTA-3'ssDNABorrelia BurgdorferiOuter Surface Protein A (OspA)Develop SERS-based aptasensor to detect OspA in serum samples.N/A10Fluorescence anisotropy (FA)N/ABiosensorRAPID-SELEX5'-DTPA dithiol and T at 86 position is replaced by 5-Octadiynyl dU moeityN/AN/AN/AN/AN/A
ABdb_1647 351046732022An antigen-targeting assay for Lyme disease: Combining aptamers and SERS to detect the OspA proteinIon-OspA-55CATGACACCGTACCTGCTCT-TGCTTTTCGTGCGCGCATAAAATACTTTGATACTGTGCCGGATGAAAGCG-AAGCACGCCAGGGACTATTA905'-CATGACACCGTACCTGCTCT-N50-AAGCACGCCAGGGACTATTA-3'ssDNABorrelia BurgdorferiOuter Surface Protein A (OspA)Develop SERS-based aptasensor to detect OspA in serum samples.N/A10Fluorescence anisotropy (FA)N/ABiosensorRAPID-SELEX5'-DTPA dithiol and T at 86 position is replaced by 5-Octadiynyl dU moeityN/AN/AN/AN/AN/A
ABdb_1664 363321352022DNA Aptamer Targets Mycobacterium tuberculosis DevR/DosR Response Regulator Function by Inhibiting Its Dimerization and DNA Binding ActivityR6DevR22 (APT-1)GTCTTGACTAGTTACGCC-CTGCTGGTCTTTAGTCTCTATTGAGTGGCTAAAGTTGAGGCAG-TCATTCAGTTGGCGCCTC795'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)DevR proteinIdentify an aptamer against DevR and inhibit DevR-dependent transcription by blocking DevR dimerisation and DNA-binding activity in Mycobacterium smegmatis.APT-1, APT-2, APT-5, and APT-7 exhibited a maximum of ∼40% inhibition of Rv1738 promoter activity.8Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ATherapeuticsSubtractive Nitrocellulose Membrane (NCM)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1665 363321352022DNA Aptamer Targets Mycobacterium tuberculosis DevR/DosR Response Regulator Function by Inhibiting Its Dimerization and DNA Binding ActivityR6DevR25 (APT-2)GTCTTGACTAGTTACGCC-CTGCCTTTTCAAAAGTTTATTGGGATGTACTTTTGTTCAGGCAG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)DevR proteinBy targeting DevR dimerization, it blocks the essential step in DevR activation, thus intercepting dormancy pathways in mycobacteria.APT-1, APT-2, APT-5, and APT-7 exhibited a maximum of ∼40% inhibition of Rv1738 promoter activity.8Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ATherapeuticsSubtractive Nitrocellulose Membrane (NCM)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1666 363321352022DNA Aptamer Targets Mycobacterium tuberculosis DevR/DosR Response Regulator Function by Inhibiting Its Dimerization and DNA Binding ActivityR6DevR28 (APT-3)GTCTTGACTAGTTACGCC-CATAAACGCAGTGACGTTCCCAGAATTGTGGCTGATGATTTTGG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)DevR proteinBy targeting DevR dimerization, it blocks the essential step in DevR activation, thus intercepting dormancy pathways in mycobacteria.N/A8Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ATherapeuticsSubtractive Nitrocellulose Membrane (NCM)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1667 363321352022DNA Aptamer Targets Mycobacterium tuberculosis DevR/DosR Response Regulator Function by Inhibiting Its Dimerization and DNA Binding ActivityR6DevR10 (APT-4)GTCTTGACTAGTTACGCC-GCGAGAATGTGCGCAAAGTCTCATGTCAGTATGTGGTCTTTTCG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)DevR proteinBy targeting DevR dimerization, it blocks the essential step in DevR activation, thus intercepting dormancy pathways in mycobacteria.APT-4 inhibited Rv1738 promoter activity in a time-dependent manner, with an inhibition value of 61% at 96 hours and inhibited DevR–DevR interaction in vivo by ∼35%.8Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ATherapeuticsSubtractive Nitrocellulose Membrane (NCM)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1668 363321352022DNA Aptamer Targets Mycobacterium tuberculosis DevR/DosR Response Regulator Function by Inhibiting Its Dimerization and DNA Binding ActivityR7DevR16 (APT-5)GTCTTGACTAGTTACGCC-CTGCCTTGGCTCGAAGGGGTGATGAGAAGTAGGCGGGAAGGCAG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)DevR proteinBy targeting DevR dimerization, it blocks the essential step in DevR activation, thus intercepting dormancy pathways in mycobacteria.APT-1, APT-2, APT-5, and APT-7 exhibited a maximum of ∼40% inhibition of Rv1738 promoter activity.8Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ATherapeuticsSubtractive Nitrocellulose Membrane (NCM)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1669 363321352022DNA Aptamer Targets Mycobacterium tuberculosis DevR/DosR Response Regulator Function by Inhibiting Its Dimerization and DNA Binding ActivityR7DevR28 (APT-6)GTCTTGACTAGTTACGCC-CGAGGGGAAGGATGGGGTGGAGGGAGGTGGGGGAGGGTTGGTGG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)DevR proteinBy targeting DevR dimerization, it blocks the essential step in DevR activation, thus intercepting dormancy pathways in mycobacteria.APT-6 inhibited Rv1738 promoter activity in a time-dependent manner, with an inhibition value of 66% at 96 hours and inhibited DevR–DevR interaction in vivo by ∼35%.8Aptamer-Linked Immobilized Sorbent Assay (ALISA)4.724 nMTherapeuticsSubtractive Nitrocellulose Membrane (NCM)-based SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1670 363321352022DNA Aptamer Targets Mycobacterium tuberculosis DevR/DosR Response Regulator Function by Inhibiting Its Dimerization and DNA Binding ActivityR7DevR29 (APT-7)GTCTTGACTAGTTACGCC-CCCAAGAACCCGCTCGCCGTGGTGACGTCGATCATGCCTTTTGG-TCATTCAGTTGGCGCCTC805'-GTCTTGACTAGTTACGCC-N44-TCATTCAGTTGGCGCCTC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)DevR proteinBy targeting DevR dimerization, it blocks the essential step in DevR activation, thus intercepting dormancy pathways in mycobacteria.APT-1, APT-2, APT-5, and APT-7 exhibited a maximum of ∼40% inhibition of Rv1738 promoter activity.8Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ATherapeuticsSubtractive Nitrocellulose Membrane (NCM)-based SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1673 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersEC1GGGGTCATAGTATCCTAGTTG-AATGATGACATTCCGAGGTACCCAGAGTGCGATACTAACCGGCCTGCTTG-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 25922)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry1.165 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1674 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersEC2GGGGTCATAGTATCCTAGTTG-CTCGCGCGCCCAATGTTTGCATCGATCTGATGGGCATAACGGCCTTGAAT-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 25922)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry10.26 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1675 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersEC3GGGGTCATAGTATCCTAGTTG-CCGACGAGACTTCCACAACGCTGCCAAGCCATCGTCCCCCCGCACTCAGG-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 25922)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry3.17 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1676 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersLM1GGGGTCATAGTATCCTAGTTG-CCGCCCGTAGAGGATTGTAGCCGCCTTTCGCCAACCTAAACCGAGACCTG-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry38.3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1677 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersLM2GGGGTCATAGTATCCTAGTTG-TCGTTCATCTGAGCTCCTGGTATCGCGTGTGTATACGAGCCTCACATTCA-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry9.03 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1678 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersLM3GGGGTCATAGTATCCTAGTTG-CCGTCCCGGTAGTCTGAGCTTACAATGTCTGATTGGAAATGCACACCAAG-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry8.89 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1680 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K3CCGGAATTCCTAATACGACTC-CCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)36,70 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1681 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K4CCGGAATTCCTAATACGACTC-CCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1682 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K6CCGGAATTCCTAATACGACTC-GCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K6 has the highest binding affinity to S. aureus BPA-12.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)17,01 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1683 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K13CCGGAATTCCTAATACGACTC-CACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)60,69 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1684 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K15CCGGAATTCCTAATACGACTC-CAGGACAGTACTCTGGACGGCAATACGTATATACGTACGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20,90 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1685 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K20CCGGAATTCCTAATACGACTC-CCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1686 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K3CCGGAATTCCTAATACGACTC-CCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)28,06 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1687 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K4CCGGAATTCCTAATACGACTC-CCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1688 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K6CCGGAATTCCTAATACGACTC-GCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)31,99 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1689 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K13CCGGAATTCCTAATACGACTC-CACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K13 and S15K15 have high binding affinity to E. coli EPEC 4.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)5,21 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1690 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K15CCGGAATTCCTAATACGACTC-CAGGACAGTACTCTGGACGGCAATACGTATATACGTACGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K13 and S15K15 have high binding affinity to E. coli EPEC 4.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)8,89 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1691 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K20CCGGAATTCCTAATACGACTC-CCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1692 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K3CCGGAATTCCTAATACGACTC-CCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K3 and S15K13 have a high binding affinity for S. agalactiae.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)6,84 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1693 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K4CCGGAATTCCTAATACGACTC-CCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1694 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K6CCGGAATTCCTAATACGACTC-GCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20,79 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1695 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K13CCGGAATTCCTAATACGACTC-CACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K3 and S15K13 have a high binding affinity for S. agalactiae.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)6,53 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1696 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K15CCGGAATTCCTAATACGACTC-CAGGACAGTACTCTGGACGGCAATACGTATATACGTACGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)45,36 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1697 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K20CCGGAATTCCTAATACGACTC-CCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1701 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS8-1CCGGAATTCCTAATACGACTC-TGCGGACTGTATCGGTCGGTCGAAAATAGTGAAGTGC-TATTGAAACGCGGCCGCGG775'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1702 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS8-4CCGGAATTCCTAATACGACTC-ACTACGCACGGCGCGAGTAAATCGATCATGGTACTGTGGC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1703 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS8-7CCGGAATTCCTAATACGACTC-GGTTCCGGTAAGATTAGATCATAACGTATGGCTAGCGCCA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1704 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS9-3CCGGAATTCCTAATACGACTC-GAAAACGTACCACTGGGATGGGTTGTGGGAGAGGGCCAGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.G-quadruplex sequence motifs are found in the S9-3, S10-10 and S10-15 aptamer sequences, with G-scores of 40, 20 and 10 (2 motifs), and 13, respectively.10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1705 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS10-1CCGGAATTCCTAATACGACTC-TATGGAGTTTGTCCGTATGATTACGTGATATCGCGACGGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1706 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS10-2CCGGAATTCCTAATACGACTC-CAGCAAACGCAGTCCAACAGCCGACAAACGGTCTTGAGGC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1707 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS10-5CCGGAATTCCTAATACGACTC-TACAAAAAGTCGTGGTCCAGTTGCCATGTGTAAAACTGTG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1708 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS10-6CCGGAATTCCTAATACGACTC-AACCAGACCACGCGAGAGGGCTCACAGTGAGACGTGAAGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1709 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS10-7CCGGAATTCCTAATACGACTC-TGGTGGGTAAAGACACCATACTGATAGTTACAAGGATGTT-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1710 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS10-8CCGGAATTCCTAATACGACTC-GCAACTTCAGTTCAGCAAGGTGCCGGCCACGCGACGGTCC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.N/A10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1711 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS10-10CCGGAATTCCTAATACGACTC-ATCCAGCAGATGTGCGCGGGTTGGTGGGGGAACGGTGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.G-quadruplex sequence motifs are found in the S9-3, S10-10 and S10-15 aptamer sequences, with G-scores of 40, 20 and 10 (2 motifs), and 13, respectively.10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1712 10.4308/hjb.29.6.789-7982022Isolation of DNA Aptamers for Enteropathogenic Escherichia coli (EPEC) Detection using Bacterial-SELEX ApproachS10-15CCGGAATTCCTAATACGACTC-GTAGCACTATAGGCAGCACGAATATGGCCGTCGGAGTTGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIdentify aptamers against pathogenic E. coli EPEC K1.1.G-quadruplex sequence motifs are found in the S9-3, S10-10 and S10-15 aptamer sequences, with G-scores of 40, 20 and 10 (2 motifs), and 13, respectively.10N/AN/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1720 348099852022Surface plasmon resonance aptasensor for Brucella detection in milkB46GGCGGCGATGAGGATGAC-GAGAGTAAAGGCCATCGGCGGCCATTTATGTTGTACCC-ACCACTGCGTGACTGCC735'-GGCGGCGATGAGGATGAC-N38-ACCACTGCGTGACTGCC-3'ssDNABrucella MelitensisWhole cellDeveloped a surface plasmon resonance (SPR) aptasensor for the detection of B. melitensis in milk samples.LOD value as low as 27 ± 11 cells.15Fluorescence Binding Assay616 ± 13 cells/mlBiosensorWhole Cell-SELEX5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1721 348099852022Surface plasmon resonance aptasensor for Brucella detection in milkB48GGCGGCGATGAGGATGAC-ACTATTTACGTTGGAACTTAAGTCCCACATGCACTGCC-ACCACTGCGTGACTGCC735'-GGCGGCGATGAGGATGAC-N38-ACCACTGCGTGACTGCC-3'ssDNABrucella MelitensisWhole cellDeveloped a surface plasmon resonance (SPR) aptasensor for the detection of B. melitensis in milk samples.N/A15Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1722 348099852022Surface plasmon resonance aptasensor for Brucella detection in milkB70GGCGGCGATGAGGATGAC-CTATAGTGCTCAAGTGCGATGCCAAGCTGGCACGATAG-ACCACTGCGTGACTGCC735'-GGCGGCGATGAGGATGAC-N38-ACCACTGCGTGACTGCC-3'ssDNABrucella MelitensisWhole cellDeveloped a surface plasmon resonance (SPR) aptasensor for the detection of B. melitensis in milk samples.B70 showed about 35% higher affinity for Brucella cells than the B46 aptamer, and the SPR sensor showed an LOD of 27 ± 11 cells.15Fluorescence Binding Assay632 ± 132 cells/mlBiosensorWhole Cell-SELEX5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1723 348099852022Surface plasmon resonance aptasensor for Brucella detection in milkB72GGCGGCGATGAGGATGAC-TCCGTGACAAGTGCGATGCCATTCCGCGTGACAGTGAT-ACCACTGCGTGACTGCC735'-GGCGGCGATGAGGATGAC-N38-ACCACTGCGTGACTGCC-3'ssDNABrucella MelitensisWhole cellDeveloped a surface plasmon resonance (SPR) aptasensor for the detection of B. melitensis in milk samples.N/A15Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1725 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA37 (SA-10R-37)GCTTCCAGCTTATTGAAT-AAAGACGGGGGGGGGGACCGGCGTATGAGTGAAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.Achieved a limit of detection (LOD) of 39 CFUs and 414 CFUs in buffer and spiked tap water samples, respectively, with the cognate pair of SA37@Cy5 and SA81@FAM.10Flow Cytometry16.5 ± 3.41 nMBiosensorWhole Cell-SELEX5'-Cyanine5 (Cy5) LabeledN/AN/ABest CandidateN/AN/A
ABdb_1726 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA81 (SA-10R-81)GCTTCCAGCTATTGAA-AACGAGGCGCAGGGGGAGGGGGTGGTACAGATAAGATGGGG-GCGCTGAAGCGCGGAAGC755'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.Achieved a limit of detection (LOD) of 39 CFUs and 414 CFUs in buffer and spiked tap water samples, respectively, with the cognate pair of SA37@Cy5 and SA81@FAM.10Flow Cytometry14.47 ± 8.18 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1727 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA25 (SA-9R-25)GCTTCCAGCTTATTGAAT-GGGGAAGGTCGTCCGACGAACCCGGTCAGATAGGGTGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry44.92 ± 1.36 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1728 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA28 (SA-10R-28)GCTTCCAGCTTATTGAAT-GCGGCCACGGAGGGGGTGCCGGGCGTGGAATAAGATGTGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry77 ± 1.22 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1729 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA35 (SA-10R-35)GCTTCCAGCTTATTGAAT-CACAGGTGTGGGGAGGTCCCCATGGAGGTGGTTCAATG-GCGCTGAAGCGCGGAAGC745'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry58.77 ± 0.73 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1730 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA40 (SA-10R-40)GCTTCCAGCTTATTGAAT-CGACGTGAAGCAATCATGGGTGGGGTACGTCGGGTCATGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry126.95 ± 0.51 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1731 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-9R-12GCTTCCAGCTTTATGAAT-TGGGCGTGGCGACGGTGTCAGGTCGGGGCGGTAAGACGAGG-GCGCTGAAGCGCGGAAGC775'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1732 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-9R-57GCTTCCAGCTTATTGAAT-GGAACGAGATGGGGCATGGCGCCACGGAGGGGAATCAAGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1733 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-1GCTTCCAGCTTATTGAAT-CGAGGGTAAGGGAAGTATGGCCGGTGCCCTGGAGTCAATG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1734 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-18GCTTCCAGCTTATTGAAT-CGGGTGGGGGGAGCGACAGACGGAAAAGCCTGGGTCAATAG-GCGCTGAAGCGCGGAAGC775'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1735 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-55GCTTCCAGCTTATTGAAT-GGAGGGGCAGGGCATGGAGCTCGACATTCATGAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1736 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-59GCTTCCAGCTTATGGAAT-GTGCCGAGGCGATACATGCACAGGCATGGTGGGATAAGGTCGGG-GCGCTGAAGCGCGGAAGC805'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1737 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-69GCTTCCAGCTTATTGAAT-CGGAGAGTCTGGCGCAGCCCTACGCCGATGTAAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1738 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-72GCTTCCAGCTTATTGAAT-CGAGGTGTGGGAGACAACGCTCCGTGACCGAGGGGAAATG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1739 https://doi.org/10.1016/j.snb.2021.1309332022Introducing an SPRi-based titration assay using aptamers for the detection of Legionella pneumophilaR10C5GCAATGGTACGGTACTTCCGGACAGTGCTGAAAACTGTGACCCCCCAAAAGTGCACGCTACTTTGCTAA69N/AssDNALegionella Pneumophila (Lp) strain Lp02Whole cellSPRi-based titration assay using Lp aptamer for detecting L. pneumophila.The limit of detection for this system was 10(4.4) cells/ml, with a linear dynamic range of 10(4.3) –10(7.7) cells/ml.N/AN/AN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/APatent application no US 16/850,355
ABdb_1748 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt1ATCCAGAGTGACGCAGCA-TGGGACGGTGCGGGGGAGGAGGATGAGCGTGGTCGTTGGGTGGCTGCCGC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence Spectroscopy214.4 nMBiosensorWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_1749 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt1_RCATCCAGAGTGACGCAGCA-GCGGCAGCCACCCAACGACCACGCTCATCCTCCTCCCCCGCACCGTCCCA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.Using SWV, exhibiting a linear detection range of 4.0 × 10(4)–7.0 × 10(7) CFU/mL, and a limit of detection of 4.0 × 10(4) CFU/mL at pH 5.0.8Fluorescence Spectroscopy3.4 nMBiosensorWhole Cell-SELEXFAM Labeled and ThiolatedN/AN/ABest CandidateN/AN/A
ABdb_1750 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt2ATCCAGAGTGACGCAGCA-TGTGGGGGCATGGGAAGGTGGGTAAGCCGTTGCGTGGGATGTGGCGGGTC-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1751 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt2_RCATCCAGAGTGACGCAGCA-GACCCGCCACATCCCACGCAACGGCTTACCCACCTTCCCATGCCCCCACA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1752 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt3ATCCAGAGTGACGCAGCA-TGGGGCGAAGGACGGTGGATGTGTGTAGGCGCGGGTGGGTCGCACGGGGT-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1753 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt3_RCATCCAGAGTGACGCAGCA-ACCCCGTGCGACCCACCCGCGCCTACACACATCCACCGTCCTTCGCCCCA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1754 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt4ATCCAGAGTGACGCAGCA-TGACAGACGGGAGAGACACGACGGGGGCGGGGAGTGAGAAGCGCGCGCTG-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1755 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt4_RCATCCAGAGTGACGCAGCA-CAGCGCGCGCTTCTCACTCCCCGCCCCCGTCGTGTCTCTCCCGTCTGTCA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1756 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt5ATCCAGAGTGACGCAGCA-TGTGGCGGTGGCAAGGTCGGTGGGTGCGGCGGCGTACCGGTGGTGGGGGG-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1757 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt5_RCATCCAGAGTGACGCAGCA-CCCCCCACCACCGGTACGCCGCCGCACCCACCGACCTTGCCACCGCCACA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1758 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt6ATCCAGAGTGACGCAGCA-TGACCGGCGGGGGGTGGTTCGCTGATAGGGCGTGTGGACCGGGGGTCGCA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1759 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt6_RCATCCAGAGTGACGCAGCA-TGCGACCCCCGGTCCACACGCCCTATCAGCGAACCACCCCCCGCCGGTCA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1760 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt7ATCCAGAGTGACGCAGCA-TGGGCGGGTAGTGGGGCAACGTGAGCGCGTGGGGTGTGGCAGGCGTGGCT-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1761 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt7_RCATCCAGAGTGACGCAGCA-AGCCACGCCTGCCACACCCCACGCGCTCACGTTGCCCCACTACCCGCCCA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1762 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt8ATCCAGAGTGACGCAGCA-AGATGACTTAGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-AATGTCGTTGGTGGCCC815'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1763 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt8_RCATCCAGAGTGACGCAGCA-AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAAGTCATCT-AATGTCGTTGGTGGCCC815'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1764 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt9ATCCAGAGTGACGCAGCA-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCATCGAGTGTA-AATGTCGTTGGTGGCCC815'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1765 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt9_RCATCCAGAGTGACGCAGCA-TACACTCGATGACACACTCTTTCCCTACACGACGCTCTTCCGATCT-AATGTCGTTGGTGGCCC815'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1766 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt10ATCCAGAGTGACGCAGCA-TGGGTGTGGTGGCGCGGGTGGCGTGGTGCGTGTACAGCTGGTTGCGGGCG-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1767 368296722023Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2Apt10_RCATCCAGAGTGACGCAGCA-CGCCCGCAACCAGCTGTACACGCACCACGCCACCCGCGCCACCACACCCA-AATGTCGTTGGTGGCCC855'-ATCCAGAGTGACGCAGCA-N50-AATGTCGTTGGTGGCCC-3'ssDNAMoraxella Catarrhalis (Bc5)Ubiquitous Surface Protein A2 (UspA2)Identify aptamers against and develop an electrochemical biosensor functionalized with an aptamer as a bioreceptor to detect UspA2.N/A8Fluorescence SpectroscopyN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1789 375919002023Aptamer-based assay for rapid detection, surveillance, and screening of pathogenic Leptospira in water samplesLAP1GGGCGTTAAAGATACAGGAACCGGTGTCGAGGTCTGAAGAATCCT455'-GAGTATCCAGACGCAGCA-N45-TGGACAGGCTTAGTCGGT-3'ssDNALeptospira InterrogansOuter membrane protein (OMP)Development of an aptamer-gold nanoparticle-based colorimetric assay for pathogenic Leptospira detection.N/A10Fluorescence SpectroscopyN/ABiosensorModified Microtiter Plate-based Cell SELEX5'-FITC LabeledN/AN/AN/AN/APatent Application No. 202241038353
ABdb_1790 375919002023Aptamer-based assay for rapid detection, surveillance, and screening of pathogenic Leptospira in water samplesLAP2CGTACGTCTGTCTCTGCATCTTATTGCTATGGAATAATGTTGTTCTGG485'-GAGTATCCAGACGCAGCA-N45-TGGACAGGCTTAGTCGGT-3'ssDNALeptospira InterrogansOuter membrane protein (OMP)Development of an aptamer-gold nanoparticle-based colorimetric assay for pathogenic Leptospira detection.N/A10Fluorescence SpectroscopyN/ABiosensorModified Microtiter Plate-based Cell SELEX5'-FITC LabeledN/AN/AN/AN/APatent Application No. 202241038353
ABdb_1791 375919002023Aptamer-based assay for rapid detection, surveillance, and screening of pathogenic Leptospira in water samplesLAP3TGGCGTTAGAGATACCGGAACCGGTGTCGGGCGTCTGAAGAATCC455'-GAGTATCCAGACGCAGCA-N45-TGGACAGGCTTAGTCGGT-3'ssDNALeptospira InterrogansOuter membrane protein (OMP)Development of an aptamer-gold nanoparticle-based colorimetric assay for pathogenic Leptospira detection.Exhibited a detection limit of 57 CFU/mL and a linear response in the concentration range of 6 × 10(5) to 60 CFU/mL.10Fluorescence Spectroscopy133.13 ± 22 nMBiosensorModified Microtiter Plate-based Cell SELEX5'-FITC LabeledN/AN/ABest CandidateN/APatent Application No. 202241038353
ABdb_1801 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB3AGCAGCACAGAGGTCAGATG-AGGCCCGGGTTTGGTTCTGGGGTTGGCGGGCTGCGTGAGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1802 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB4AGCAGCACAGAGGTCAGATG-GAAGGGTTTGGTGGTAAATTGCGTGGTTGGCTGTTGATGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1803 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB6AGCAGCACAGAGGTCAGATG-GGCGGGTTTGGATCTTTGGTTGGCGCTTGTTTCTTTATGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1804 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB7AGCAGCACAGAGGTCAGATG-TCGGTCGGGTTTGGGTGTTGGTTGGCGATAAAGATAACTG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow Cytometry19.64 ± 4.11 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1805 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB11AGCAGCACAGAGGTCAGATG-GTGTAGGTCAATCGACGCCACCCATAGCAACCATCCTGCG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1806 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB14AGCAGCACAGAGGTCAGATG-GTGGGCAGTGGGGGGAGGGGGAGGTGGAGGTTAGACGTGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow Cytometry28.64 ± 3.10 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1807 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB15AGCAGCACAGAGGTCAGATG-GGCGGGTTTGGATCTTTGGTTGGCGCCTGTTTCTTTATGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.Achieve highly sensitive detection of B. cereus as low as 10 cfu/mL.16Flow Cytometry16.13 ± 4.98 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1808 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB16AGCAGCACAGAGGTCAGATG-ATATGTTTACGCCAGTGGTATTATTGGGGTTGATATGTCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.Achieve highly sensitive detection of B. cereus as low as 10 cfu/mL.16Flow Cytometry20.67 ± 5.23 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1810 374372662023Specific Instantaneous Detection of Klebsiella pneumoniae for UTI Diagnosis with a Plasmonic Gold Nanoparticle Conjugated AptasensorKPBA1GGCTGGATGGGGCGTGT-GGAGCCCCGTTAGAATATCAGAGGTGGTGG-CAACGGTGCGGACAGCG645'-GGCTGGATGGGGCGTGT-30N-CAACGGTGCGGACAGCG-3'ssDNAKlebsiella Pneumoniae (MTCC-7028)Whole cellDeveloped localized surface plasmon resonance (LSPR) aptasensor using a tailor-made plasmonic aptamer-gold nanoparticle (AuNP) for detection of Klebsiella pneumoniae.LoD as low as 3.4 × 10(3) CFU/mL within 5 min.10Flow CytometryN/ABiosensorWhole Cell-SELEX5′-Thiolated (5′-/5ThioMC6-D) and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1829 374294292024Assessment of the growth inhibition and anti-biofilm activity of aptamer (PmA2G02) against Proteus mirabilis 1429TPmA2G02ATTACTTACGCTATCTAAttttGCTGTAGGGGAGGAGGGTGGGTtttTTATCATCTGGTATGTTA65N/AssDNAProteus Mirabilis (1429T)BiofilmInhibit biofilm formation, adhesion, and mobility, along with a reduction in biofilm-related genes, namely rsbA, fliC2 & fimD, in P. mirabilis.Aptamer treatment resulted in a 50% reduction in biofilm thickness and a 2.29-fold and 1.34-fold decrease in mRNA expression of the fliC2 and fimD genes, respectively.N/AN/AN/ATherapeuticsWhole Cell-SELEXN/AAptamer treatment did not significantly affect cell viability.N/AN/AN/AN/A
ABdb_1832 388981152024Cell-SELEX for aptamer discovery and its utilization in constructing electrochemical biosensor for rapid and highly sensitive detection of Legionella pneumophila serogroup 1AY-19TCCCTACGGCGCTAAC-GACATGATGGTGCCAAACAACATTCGGAAAGCCTGACCGT-CCACCGTGCTACAAC715'-TCCCTACGGCGCTAAC-N40-CCACCGTGCTACAAC-3'ssDNALegionella Pneumophila serogroup 1 (L. pneumophila SG1) (ATCC 33152)Whole cellIdentify an aptamer against L. pneumophila SG1 and develop an electrochemical aptasensor for its detection.Limit of Detection (LOD): 4.6 CFU/mL and linear trend ranging from 10 to 10(8) CFU/mL.10Fluorescence Binding Assay14.19 nMDetectionWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1833 388981152024Cell-SELEX for aptamer discovery and its utilization in constructing electrochemical biosensor for rapid and highly sensitive detection of Legionella pneumophila serogroup 1AY-3TCCCTACGGCGCTAAC-TGGCAAATAATGCAATTGAAGAAAGCCCCCCCCTGCCCGA-CCACCGTGCTACAAC715'-TCCCTACGGCGCTAAC-N40-CCACCGTGCTACAAC-3'ssDNALegionella Pneumophila serogroup 1 (L. pneumophila SG1) (ATCC 33152)Whole cellIdentify an aptamer against L. pneumophila SG1 and develop an electrochemical aptasensor for its detection.N/A10Fluorescence Binding Assay15.61 nMDetectionWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1834 388981152024Cell-SELEX for aptamer discovery and its utilization in constructing electrochemical biosensor for rapid and highly sensitive detection of Legionella pneumophila serogroup 1AY-29TCCCTACGGCGCTAAC-TGTGAAACAAGGCAAGGGAACTGACGTCATAAGGATAGCA-CCACCGTGCTACAAC715'-TCCCTACGGCGCTAAC-N40-CCACCGTGCTACAAC-3'ssDNALegionella Pneumophila serogroup 1 (L. pneumophila SG1) (ATCC 33152)Whole cellIdentify an aptamer against L. pneumophila SG1 and develop an electrochemical aptasensor for its detection.N/A10Fluorescence Binding Assay17.38 nMDetectionWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1835 388981152024Cell-SELEX for aptamer discovery and its utilization in constructing electrochemical biosensor for rapid and highly sensitive detection of Legionella pneumophila serogroup 1AY-37TCCCTACGGCGCTAAC-AAGATGAAAGACGACCGGACAGTACATATAGCGCTCTCGC-CCACCGTGCTACAAC715'-TCCCTACGGCGCTAAC-N40-CCACCGTGCTACAAC-3'ssDNALegionella Pneumophila serogroup 1 (L. pneumophila SG1) (ATCC 33152)Whole cellIdentify an aptamer against L. pneumophila SG1 and develop an electrochemical aptasensor for its detection.N/A10Fluorescence Binding Assay17.96 nMDetectionWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1836 388981152024Cell-SELEX for aptamer discovery and its utilization in constructing electrochemical biosensor for rapid and highly sensitive detection of Legionella pneumophila serogroup 1AY-31TCCCTACGGCGCTAAC-ACCTGCAGTAGGGAATGCGAATGATGAGACGCCTGATTGG-CCACCGTGCTACAAC715'-TCCCTACGGCGCTAAC-N40-CCACCGTGCTACAAC-3'ssDNALegionella Pneumophila serogroup 1 (L. pneumophila SG1) (ATCC 33152)Whole cellIdentify an aptamer against L. pneumophila SG1 and develop an electrochemical aptasensor for its detection.N/A10Fluorescence Binding Assay68.83 nMDetectionWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1837 388981152024Cell-SELEX for aptamer discovery and its utilization in constructing electrochemical biosensor for rapid and highly sensitive detection of Legionella pneumophila serogroup 1AY-24TCCCTACGGCGCTAAC-ACAGACAGAATGAGTGAACATAGGCCAATAACGCACGTCC-CCACCGTGCTACAAC715'-TCCCTACGGCGCTAAC-N40-CCACCGTGCTACAAC-3'ssDNALegionella Pneumophila serogroup 1 (L. pneumophila SG1) (ATCC 33152)Whole cellIdentify an aptamer against L. pneumophila SG1 and develop an electrochemical aptasensor for its detection.N/A10Fluorescence Binding Assay75.24 nMDetectionWhole Cell-SELEX5'-Thiolated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1856 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterAb1 (A00)TGGTGCGTGCTATTCAGAGT-GAGGGACGCATGATTGGGTGACTCCGGGAGATCATGCAAG-GAACCTGTAGCCACGAATAC805'-TGGTGCGTGCTATTCAGAGT-N40-GAACCTGTAGCCACGAATAC-3'ssDNAAliarcobacter Butzerli (ATCC 49616)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay14.12 ± 2.31 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1857 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterAb2TGGTGCGTGCTATTCAGAGT-GGGGTGGACCGGGGGGATCGAATAACGCATCGCGCCTAGT-GAACCTGTAGCCACGAATAC805'-TGGTGCGTGCTATTCAGAGT-N40-GAACCTGTAGCCACGAATAC-3'ssDNAAliarcobacter Butzerli (ATCC 49616)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay26.26 ± 9.87 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1858 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterAb3TGGTGCGTGCTATTCAGAGT-CCTCTAGGCGGTAAGGATCGGACCTTGGTCTGATGGTGAG-GAACCTGTAGCCACGAATAC805'-TGGTGCGTGCTATTCAGAGT-N40-GAACCTGTAGCCACGAATAC-3'ssDNAAliarcobacter Butzerli (ATCC 49616)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay66.51 ± 24.28 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1859 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterAb4TGGTGCGTGCTATTCAGAGT-GACGGATACGGATTCAGGGCGGAGTGCCAAGATACGGATG-GAACCTGTAGCCACGAATAC805'-TGGTGCGTGCTATTCAGAGT-N40-GAACCTGTAGCCACGAATAC-3'ssDNAAliarcobacter Butzerli (ATCC 49616)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay97.98 ± 37.42 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1860 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterAb5TGGTGCGTGCTATTCAGAGT-GTTGAGGATTAGGATCGCTTTAAACGATTAGGATCCGAAT-GAACCTGTAGCCACGAATAC805'-TGGTGCGTGCTATTCAGAGT-N40-GAACCTGTAGCCACGAATAC-3'ssDNAAliarcobacter Butzerli (ATCC 49616)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay142.2 ± 39.3 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1861 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterA01GCGCGGGCTATTCAGAGTGAGGGCCGCGTGAGCGGGCGACTCCGGGAGATCGCGCGCG58N/AssDNAAliarcobacter Butzerli (ATCC 49616)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay27.91 ± 13.34 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1862 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterA02GAGCGGGCGACTCCGGGAGATCGCGCGCGG30N/AssDNAAliarcobacter Butzerli (ATCC 49616)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.Exhibited a wide detection range of up to 1 × 10(7) CFU/mL and the lowest limit of detection (LOD) of 1 CFU/mL, for each bacterium.12Fluorescence Binding Assay6.86 ± 5.77 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1863 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterA03CGACTCCGGGAGATCG16N/AssDNAAliarcobacter Butzerli (ATCC 49616)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay19.33 ± 9.76 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1864 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterC00GTCCTGAGACTCATGTCTGCTGTCAATCGCAAGGTCCATGTCCTGAGACTCATGTCTGCTGTCAATCGCAAGGTCCA77N/AssDNACampylobacter Jejuni (ATCC 33560)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay48.12 ± 6.15 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1865 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterC01CGCGTGTCTGCTGTCAATCGCAAGGTCCATGTCCCGCG38N/AssDNACampylobacter Jejuni (ATCC 33560)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.Exhibited a wide detection range of up to 1 × 10(7) CFU/mL and the lowest limit of detection (LOD) of 1 CFU/mL, for each bacterium.12Fluorescence Binding Assay27.69 ± 5.66 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1866 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterC02CGCGAGTCTGCTGTCAATCGCAAGGCGCG29N/AssDNACampylobacter Jejuni (ATCC 33560)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay61.58 ± 19.805 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1867 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterC03CGCTGTCAATCGCG14N/AssDNACampylobacter Jejuni (ATCC 33560)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay240.2 ± 106.85 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1868 376194712024A biosensor encompassing fusarinine C-magnetic nanoparticles and aptamer-red/green carbon dots for dual-channel fluorescent and RGB discrimination of Campylobacter and AliarcobacterC04GCGTCCATGTCGC13N/AssDNACampylobacter Jejuni (ATCC 33560)Whole cellDeveloped a bi-module biosensor using FsC-MNPs and aptamer-CDs to discriminate Campylobacter and Aliarcobacter.N/A12Fluorescence Binding Assay259.8 ± 55.1 nMBiosensorWhole Cell-SELEX with the High-Throughput Sequencing (HTS)5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1873 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 3 (Ap3)NNNNNNNNNNNNNNNNNNNNNGGGGGCTTTCAAGGGCGCGACGAAGCTGTTTTATTCTC59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.Achieved a detection limit of 0.1 ag/mL EC proteins (equivalent to 3 protein particles per mL) within 120 min from the plasma sample.15Isothermal Titration Calorimetry (ITC)8.30 x 10-7 MBiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_1874 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 1 (Ap1)GACGTTTTGCAAGTGCGGCTACTACAAGCGAAGTTCCATCATTTTAGAGTCATAAACGC59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1875 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 6 (Ap6)CCGCCTGGAAGGCATTTCGCCGGGAAGACCCACGTCACTCCTCCCCTGAACTTCGGTTC59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1876 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 2 (Ap2)TATACATTGATTTATCGGACCACTGCAGTACCACGCAGGATGGACGGCCATCGTTAATA59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1877 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 4 (Ap4)TAGGATTCGTTTATTCGTGGGGCTGTAGTTCCGATAAAACCGCCAAGGGATCGTTGCCT59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1878 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 5 (Ap5)ATTAGATGTAAAACGCCACGGGTAGCAGTTAAAGTCGTATTTATAGGCACAACGAAGAT59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1879 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 7 (Ap7)CAATTAGACAGGCTCTAAGCTTTGGACCCAGCGTAGAAAGTTTGTTATTTCTTCGGGCT59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1880 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 8 (Ap8)ATAAGCTTTTTAAGGCAAGGACGCGGCCCGGTACTTGCGTTGGAGGAATCTTAAGTTAT59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1881 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 9 (Ap9)CATTTATAACTACGATTGTGGTCACATTCCGTGGATGGAATCACTTCTCAGGTGCCGAG59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1882 403598082025Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)Aptamer 10 (Ap10)TGTTTACACATATTCTTGCGCATTACTTGCTTTGGCATTATCTGTATCCCCCTCAGCGG59N/AssDNAMycobacterium Tuberculosis (H37Rv)ESAT6/CFP10 fusion proteins (EC proteins)Develop a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing) assay designed for the rapid and sensitive detection of M.tb. antigens from peripheral blood.N/A15Isothermal Titration Calorimetry (ITC)N/ABiosensorMagnetic Bead (MB)-based SELEXN/AN/AN/AN/AN/AN/A
ABdb_1883 414312702025Bioimaging with fluorescent nucleic-acid aptamers for the specific detection and quantification of Pseudomonas aeruginosa alone and in heterogeneous bacterial populationsF23ATACCAGCTTATTCAATT-CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG-AGATAGTAAGTGCAATCT96N/AssDNAPseudomonas Aeruginosa (ATCC 15692)Whole cellDevelop a fluorescent aptamer for detecting Pseudomonas aeruginosa.73.79% of live P. aeruginosa cells (3102/4204) were labeled by the Cy5‐F23 aptamer, compared to only 0.06% of live S. aureus (4/6767).15Flow Cytometry17.27 ± 5.00 nMImagingWhole Cell-SELEX5'-Cyanine5 (Cy5) LabeledN/AThe aptamer remains highly stable for 72 hours.N/AN/AN/A
ABdb_1884 404519602025Screening and characterization of nucleic acid aptamers targeting Brucella surface antigens: identification and analysis of their corresponding binding sitesWS-6GCTGCAATACTCATGGACAGCACGGCACGGGCTGTCGATCTCGAGGGAAACGGCACATGTGTCTGGAGTACGACCCTGAA80N/AssDNABrucella S2 vaccine strainOuter membrane protein BamA (β-barrel assembly machinery A)Identify aptamers against Brucella and conjugate them to liposomes for selective recognition and significantly reduced bacterial invasion of host cells.WS-6 significantly reduced Brucella invasion, as evidenced by a decrease in qPCR Ct from 22.34 to 20.64 (P < 0.05), confirming a reduced intracellular bacterial load.13Indirect Enzyme-Linked Aptamer Assay (i-ELAA)16.23 ± 5.84 nMTherapeuticsWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AThe aptamer demonstrated stable binding to the Brucella S2 vaccine across a temperature range of 16–50°C, with optimal binding observed between 16°C and 50°C and under neutral conditions (pH 7.6) in 2h.Best CandidateN/AN/A
ABdb_1885 404519602025Screening and characterization of nucleic acid aptamers targeting Brucella surface antigens: identification and analysis of their corresponding binding sitesWS-26GCTGCAATACTCATGGACAGCGGAACGCTGTCTCCTCATTCAATCTAGGAGTCTGCGTGAGTCTGGAGTACGACCCTGAA80N/AssDNABrucella S2 vaccine strainWhole cellBinds BamA β-barrel assembly protein → blocks/inhibits bacterial invasion into host cells.N/A13Indirect Enzyme-Linked Aptamer Assay (i-ELAA)20.33 ± 8.12 nMTherapeuticsWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1886 404519602025Screening and characterization of nucleic acid aptamers targeting Brucella surface antigens: identification and analysis of their corresponding binding sitesWS-17GCTGCAATACTCATGGACAGGACAACGTCCTGTACCAGCGCTACGCCAGATCAGTTTTAGGTCTGGAGTACGACCCTGAA80N/AssDNABrucella S2 vaccine strainWhole cellBinds BamA β-barrel assembly protein → blocks/inhibits bacterial invasion into host cells.N/A13Indirect Enzyme-Linked Aptamer Assay (i-ELAA)17.11 ± 6.35 nMTherapeuticsWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1887 404519602025Screening and characterization of nucleic acid aptamers targeting Brucella surface antigens: identification and analysis of their corresponding binding sitesWS-32GCTGCAATACTCATGGACAGGGGACTCAGGTCCCCGGGAGCGCAATGGTGGGGTTTTAATGTCTGGAGTACGACCCTGAA80N/AssDNABrucella S2 vaccine strainWhole cellBinds BamA β-barrel assembly protein → blocks/inhibits bacterial invasion into host cells.N/A13Indirect Enzyme-Linked Aptamer Assay (i-ELAA)13.08 ± 2.97 nMTherapeuticsWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1888 404519602025Screening and characterization of nucleic acid aptamers targeting Brucella surface antigens: identification and analysis of their corresponding binding sitesWS-44GCTGCAATACTCATGGACAGGGACCTGTCCCTGGCCTCTTAGGGTAAAATTTTGCCACAAGTCTGGAGTACGACCCTGAA80N/AssDNABrucella S2 vaccine strainWhole cellBinds BamA β-barrel assembly protein → blocks/inhibits bacterial invasion into host cells.N/A13Indirect Enzyme-Linked Aptamer Assay (i-ELAA)15.03 ± 4.2 nMTherapeuticsWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1889 398941032025Introduce a novel, extremely sensitive aptamer against staphylococcal enterotoxin type DAptamer 1CCTAACCGATATCACACTCAC-GGGTGCAGCCCGGAGCGGCTTGCATGATTGGGTTGGTGGG-GTTGGTCGTCATTGGAGTATC825'-CCTAACCGATATCACACTCAC-N40-GTTGGTCGTCATTGGAGTATC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin D (SED)Isolate high-affinity ssDNA aptamers with specificity for SED.The limit of detection (LOD) for SED was determined to be 45 nM.10Surface Plasmon Resonance (SPR) and Enzyme-Linked Apta-Sorbent Assay (ELASA)4.4 ± 2.26 nM (by SPR) and 13.43 nM (by ELASA)DiagnosticWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1896 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS8CGTACGGTCGACGCTAGC-ACACCCCCCAGGATCATAAATTCTCTCGCTGTAGC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1897 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS10CGTACGGTCGACGCTAGC-ACCAGCCATAGTCACATCAAAACTAACTCACATTC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1898 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS12CGTACGGTCGACGCTAGC-CCCATACTCATCACCATTCACATCACTCACCTAGC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1899 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS1GGATCCGAGCTCCACGTG-GCTAGGTGAGTGATGTGAATGGTGATGAGTATGGG-GCTAGCGTCGACCGTACG715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S1, the Ru-modified oligonucleotide, bound S. pneumoniae R6 with slightly lower affinity than S9.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry125 ± 91 nMDetectionSELEX and Mod-SELEXT=dURu(bpy)TP1, 5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1900 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS9ACACCCCCCAGGATCATAAATTCTCTCGCTGTAGC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S9 bound better to the bacterial target than the modified sequence S1.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry118 ± 1 nMDetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1901 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS11ACCAGCCATAGTCACATCAAAACTAACTCACATTC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S11 exhibited a lower propensity at binding to the bacterial target.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry541 ± 1 nMDetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1902 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS13CCCATACTCATCACCATTCACATCACTCACCTAGC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1915 400169202025Cascaded Strand Displacement Amplification and CRISPR/Cas12a Aptasensor Utilizing MoS2 Nanoflowers for Colorectal Cancer Biomarker Porphyromonas gingivalis DetectionApt-Pg1TGACTGACGACGACTC-AGTGGAGTATCGCCTTGCCGACCCGGGTGTCTACGATCAGTGAGTGGTAGT-GACTGCTCGAGCTG815'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellIdentify specific aptamers targeting P. gingivalis and develop an aptasensor based on MoS2 nanoflowers that integrates strand displacement amplification and CRISPR/Cas12a double-amplification for detection.N/A15Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1916 400169202025Cascaded Strand Displacement Amplification and CRISPR/Cas12a Aptasensor Utilizing MoS2 Nanoflowers for Colorectal Cancer Biomarker Porphyromonas gingivalis DetectionApt-Pg2TGACTGACGACGACTC-TGGAGCTCGGGTATTCTCGTCAGACCCTCCTGAGTTGATTTTAGCAACCG-GACTGCTCGAGCTG805'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellIdentify specific aptamers targeting P. gingivalis and develop an aptasensor based on MoS2 nanoflowers that integrates strand displacement amplification and CRISPR/Cas12a double-amplification for detection.N/A15Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1917 400169202025Cascaded Strand Displacement Amplification and CRISPR/Cas12a Aptasensor Utilizing MoS2 Nanoflowers for Colorectal Cancer Biomarker Porphyromonas gingivalis DetectionApt-Pg3TGACTGACGACGACTC-CGGCGAGTAACGACTATGTCGCACGGGTGTCTTACGAGACGGTTGGGGTC-GACTGCTCGAGCTG805'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellIdentify specific aptamers targeting P. gingivalis and develop an aptasensor based on MoS2 nanoflowers that integrates strand displacement amplification and CRISPR/Cas12a double-amplification for detection.N/A15Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1918 400169202025Cascaded Strand Displacement Amplification and CRISPR/Cas12a Aptasensor Utilizing MoS2 Nanoflowers for Colorectal Cancer Biomarker Porphyromonas gingivalis DetectionApt-Pg4TGACTGACGACGACTC-CTTGTCCATGGCTTGTCCACTCGGGTGTCTGGACAATGAAACCGAACTGC-GACTGCTCGAGCTG805'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellIdentify specific aptamers targeting P. gingivalis and develop an aptasensor based on MoS2 nanoflowers that integrates strand displacement amplification and CRISPR/Cas12a double-amplification for detection.N/A15Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1919 400169202025Cascaded Strand Displacement Amplification and CRISPR/Cas12a Aptasensor Utilizing MoS2 Nanoflowers for Colorectal Cancer Biomarker Porphyromonas gingivalis DetectionApt-Pg8TGACTGACGACGACTC-AGAACGCTTTATGCGCACGGGGGACTTCGGAGTCGTCGAGTACGGGTACC-GACTGCTCGAGCTG805'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellIdentify specific aptamers targeting P. gingivalis and develop an aptasensor based on MoS2 nanoflowers that integrates strand displacement amplification and CRISPR/Cas12a double-amplification for detection.N/A15Fluorescence Binding Assay183.79 ± 3.27 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1920 400169202025Cascaded Strand Displacement Amplification and CRISPR/Cas12a Aptasensor Utilizing MoS2 Nanoflowers for Colorectal Cancer Biomarker Porphyromonas gingivalis DetectionApt-Pg8ATGACTGACGACGACTCAGAACGCTTTATGCGCACGGGGGACTTCGGAGTCGTCGAGT575'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAPorphyromonas Gingivalis (ATCC 33277)Whole cellIdentify specific aptamers targeting P. gingivalis and develop an aptasensor based on MoS2 nanoflowers that integrates strand displacement amplification and CRISPR/Cas12a double-amplification for detection.Achieving a limit of detection of 10 CFU/mL.15Fluorescence Binding Assay133.15 ± 48.56 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1921 400531472025Fluorescent aptasensor for detection of Salmonella typhimurium using boric acid-functionalized terbium metal-organic framework and magnetic nanoparticlesAptamerTAGGGAAGAGAAGGACATATGATCAGGAGTCATGCTACCACTGGTGATTACGCCTCGCTCAGCTTGACTAGTACATGACCACTTGA86N/AssDNASalmonella Typhimurium (S. Typhimurium) (MTCC 3232)Whole cellA fluorescent detection platform was developed using boric acid-functionalized terbium metal–organic framework (BA-Tb-MOF) and carboxyl-modified magnetic nanoparticles (MNPs) to identify S. typhimurium.Showed an inverse linear relationship within the range of 10(1)–10(9) CFU/mL, and the detection limit was 4 CFU/mL.N/AN/AN/ABiosensorWhole Cell-SELEX5'-FAM Labeled and 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1922 399057042025Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micraApt-ss1TGACTGACGACGACTC-CCCGCAGGGGATCCGGATGCCGCGTTGGAGGAGATATGTATTATTCCATC-GACTGCTCGAGCTG805'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAParvimonas Micra (ATCC 33270)Whole cellIsolate aptamers against P. micra and develop colorimetric aptsensor using Au@Fe3O4 nanoparticles.Demonstrated a linear response across a range of 10–10(8) CFU/mL for P. micra with a limit of detection of 11 CFU/mL.17Fluorescence Binding Assay33.84 ± 0.92 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1923 399057042025Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micraApt-ss2TGACTGACGACGACTC-GGAGAACTACCGCACCAACAATTCACCGTCTGGACGTTCTGCCCTCTCCC-GACTGCTCGAGCTG805'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAParvimonas Micra (ATCC 33270)Whole cellIsolate aptamers against P. micra and develop colorimetric aptsensor using Au@Fe3O4 nanoparticles.N/A17Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-Thiolated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1924 399057042025Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micraApt-ss8TGACTGACGACGACTC-GGGAGGTCAGACTATCTTGTTCCTCCTGGAGGTCGGGAAGAGAGTTGACG-GACTGCTCGAGCTG805'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAParvimonas Micra (ATCC 33270)Whole cellIsolate aptamers against P. micra and develop colorimetric aptsensor using Au@Fe3O4 nanoparticles.N/A17Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-Thiolated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1925 399057042025Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micraApt-ss9TGACTGACGACGACTC-GGGGGCTGTCAAAAATTACTCTTGGGCCCAGCAATGGTAATTTTCTGACC-GACTGCTCGAGCTG805'-TGACTGACGACGACTC-N50-GACTGCTCGAGCTG-3'ssDNAParvimonas Micra (ATCC 33270)Whole cellIsolate aptamers against P. micra and develop colorimetric aptsensor using Au@Fe3O4 nanoparticles.N/A17Fluorescence Binding AssayN/ABiosensorWhole Cell-SELEX5'-Thiolated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1934 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 7.1ACACTAGATCAGTCACAG-GATTAAGGATCGTTGTTGGGTGGTTTCCGTAATTACTATC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1935 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 15.1ACACTAGATCAGTCACAG-GGTTGCTCAGGAGAAGGGAACGTGGGTGGTGGCGCGGACG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1936 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 3.1ACACTAGATCAGTCACAG-ACGGGGAGAGGGTCATCTCTGGTTTCGGGGTGCTAATTCT-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1937 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 8.1ACACTAGATCAGTCACAG-CCCCGACCTGTACACGTACTACCCGATGACATTTGGTGTT-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1938 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 13.1ACACTAGATCAGTCACAG-TGGACGCGGCGGGCCGGATTAAGTTGAGGAGAGGGGGGTG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1939 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 16.1ACACTAGATCAGTCACAG-AGTATATATGGATTCCGCAACGTACGCAGGGGGTGTCGCC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1940 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 14.1ACACTAGATCAGTCACAG-CAGAGAGGGGCGGCGAGGGACAAAGGAGGGTAGGTGTGCC-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1941 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 12.1ACACTAGATCAGTCACAG-ATCACAGCGGAGGTTTAACGTGTGGCGGGTGTGGGGACATG-GACTTGACTCAGACATCT775'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1942 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 11.1ACACTAGATCAGTCACAG-GAGCAACAGTCTCGCCTTCGTTACTGCGCGCGGGACGGGG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.CA 11.1 exhibited S. aureus binding in a linear range of 10(3) to 10(8) CFU/mL and a limit of detection (LOD) of 10(3) CFU/mL.11Bead-based Binding Assay263.8 ± 78 nMDetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1943 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 17.1ACACTAGATCAGTCACAG-AAGTGGAATCAAGCATGGGTATTTTAAGCATCGTATCTCG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1944 415331982026Fluorescent aptasensor targeting the fibrinogen-binding domain of clumping factor A for rapid detection of Staphylococcus aureusCA 5.1ACACTAGATCAGTCACAG-TGTTTCACGGGGGGGAGGGTAACGGAAGCTAATGGTCTCG-GACTTGACTCAGACATCT765'-ACACTAGATCAGTCACAG-N40-GACTTGACTCAGACATCT-3'ssDNAStaphylococcus aureus (S. aureus)Clumping factor A (ClfA)Identify aptamers specific to ClfA and develop a fluorescence assay on the GO platform for its detection.N/A11N/AN/ADetectionNi-NTA affinity SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1951 104519131999In vitro selection of DNA aptamers to anthrax spores with electrochemiluminescence detectionAptamer poolN/AN/AN/AssDNABacillus Anthracis (BA)Anthrax sporesDevelop an aptamer–magnetic bead-electrochemiluminescence (AM-ECL) sandwich assay for detecting anthrax spores.Display broad dynamic range equivalent to 10– 6×10(6) anthrax spores.4ECL-based and Colorimetric Peroxidase-Based Aptamer Plate Binding AssayN/ADetectionSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1952 118086912002Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methodsAnti-cholera whole toxin aptamersN/AN/A5'-CCGGATCCGTTGATA-N20-GTGGTGTTGGCTCCC-3'ssDNAVibrio CholeraeCholera toxinDeveloped an aptamer-based electrochemiluminescence assay using a dsDNA aptamer for biotoxin detection.Detection limit of less than 40 ng in the cholera electrochemiluminescence assay.5N/AN/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated (for ECL) or 5'-Amidation (NH₂-(CH2)6) (for colorimetric assays)N/AN/AN/AN/AN/A
ABdb_1953 118086912002Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methodsAnti-staphylococcal enterotoxin B (SEB) aptamersN/AN/A5'-CCGGATCCGTTGATA-N20-GTGGTGTTGGCTCCC-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)Developed an aptamer-based electrochemiluminescence assay using a dsDNA aptamer for biotoxin detection.Detection limit of less than 10 pg SEB with electrochemiluminescence assay.5N/AN/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated (for ECL) or 5'-Amidation (NH₂-(CH2)6) (for colorimetric assays)N/AN/AN/AN/AN/A
ABdb_1954 165501912006Anti-Francisella tularensis DNA aptamers detect tularemia antigen from different subspecies by Aptamer-Linked Immobilized Sorbent AssayAptamer cocktailN/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAFrancisella Tularensis subspecies (subsp) JaponicaTularemia Bacterial AntigenIdentify aptamers that bind specifically to the tularemia bacterial antigen from the subspecies japonica or holarctica.The detection limit for the aptamer cocktail is 25 ng (1.7 × 10^3/ml of bacteria).10Aptamer-Linked Immobilized Sorbent Assay (ALISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1955 174422752007Aptamer from whole-bacterium SELEX as new therapeutic reagent against virulent Mycobacterium tuberculosisNK2N/AN/A5'-GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Membrane proteinIdentify an aptamer that binds to and improves CD4+T cells & produces IFN-γ by blocking some membrane proteins of H37Rv, decreases the bacterial number, and prolongs the survival rate in the mouse model by inhibiting the invasion of M. tuberculosis into phagocytes and CD8+T cells.Intracellular IFN-γ levels in CD3+CD4+ T cells increased from approximately 35% (without aptamer) to approximately 55% (with the NK2). The half-life of survival in mice was prolonged by 3 days with a single injection of NK2 aptamer treatment.10Isothermal Titration Calorimetry (ITC)N/ATherapeuticsWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1956 173443502007Development and characterization of monoclonal antibodies and aptamers against major antigens of Mycobacterium avium subsp. paratuberculosisAptamer 94N/AN/A5'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40-ATTTCTCCTACTGGGATAGGTGGATTAT-3'ssDNAMycobacterium Avium subsp. paratuberculosis (ATCC 19698)MAP0105c gene productIdentify aptamers that were screened against the MAP0105c gene product.Bound specifically to the N-terminal half of MAP0105c, and nearly all mycobacteria tested.12N/AN/ADetectionCounter-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1957 190528512009Plastic-adherent DNA aptamer-magnetic bead and quantum dot sandwich assay for Campylobacter detectionC2 and C3N/AN/AN/AssDNACampylobacter Jejuni (ATCC 29428)Surface proteinIdentify aptamers against surface proteins from C. jejuni and develop a sandwich assay ( (C2 aptamer-MB-bacteria-C3 aptamer-QD complexes) for its detection.Exhibits detection limits as low as an average of 2.5 cfu equivalents in buffer and 10–250 cfu in various food matrices.5N/AN/ABiosensorSELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1958 205825872010Selection and characterization of DNA aptamers with binding selectivity to Campylobacter jejuni using whole-cell SELEXONS-23N/AN/A5'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNACampylobacter Jejuni (A9a)Whole cell (bind via proteinaceous nature for cell surface target)Identify aptamers against C. jejuni and detect them in a mixed cell population.ONS-23 showed high binding affinity (25–36%) for all other C. jejuni strains screened (inclusivity) and low apparent binding affinity (1–5%) with non-C. jejuni strains (exclusivity).10Flow Cytometry292.8 ± 53.1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AWO Patent WO/2011/097420
ABdb_1959 230564922012Selection and application of ssDNA aptamers to detect active TB from sputum samplesCSIR 2.11N/AN/A5'-GCCTGTTGTGAGCCTCCTAAC-N49-CATGCTTATTCTTGTCTCCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)CFP-10 and ESAT-6 monomer and heterodimerIdentify aptamers against the CFP-10.ESAT-6 heterodimer and active TB from human sputum samples.CSIR 2.11 achieved 100% sensitivity and 68.75% specificity in detecting antigen in sputum samples.6Surface Plasmon Resonance (SPR)8 ± 1.07 nMDetectionSELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1960 230564922012Selection and application of ssDNA aptamers to detect active TB from sputum samplesCSIR 2.19N/AN/A5'-GCCTGTTGTGAGCCTCCTAAC-N49-CATGCTTATTCTTGTCTCCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)CFP-10 and ESAT-6 monomer and heterodimerIdentify aptamers against the CFP-10.ESAT-6 heterodimer and active TB from human sputum samples.N/A6Surface Plasmon Resonance (SPR)1.6 ± 0.5 nMDetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_1961 230564922012Selection and application of ssDNA aptamers to detect active TB from sputum samplesCSIR 2.2N/AN/A5'-GCCTGTTGTGAGCCTCCTAAC-N49-CATGCTTATTCTTGTCTCCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)CFP-10 and ESAT-6 monomer and heterodimerIdentify aptamers against the CFP-10.ESAT-6 heterodimer and active TB from human sputum samples.N/A6Surface Plasmon Resonance (SPR)21.5 ± 4.3 nMDetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_1962 230564922012Selection and application of ssDNA aptamers to detect active TB from sputum samplesCSIR 2.15N/AN/A5'-GCCTGTTGTGAGCCTCCTAAC-N49-CATGCTTATTCTTGTCTCCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)CFP-10 and ESAT-6 monomer and heterodimerIdentify aptamers against the CFP-10.ESAT-6 heterodimer and active TB from human sputum samples.N/A6Surface Plasmon Resonance (SPR)12 ± 1.6 nMDetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_1963 230564922012Selection and application of ssDNA aptamers to detect active TB from sputum samplesCSIR 2.21N/AN/A5'-GCCTGTTGTGAGCCTCCTAAC-N49-CATGCTTATTCTTGTCTCCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)CFP-10 and ESAT-6 monomer and heterodimerIdentify aptamers against the CFP-10.ESAT-6 heterodimer and active TB from human sputum samples.N/A6Surface Plasmon Resonance (SPR)10.2 ± 3.4 nMDetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_1964 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK2N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Significantly decreased macrophage invasion from 64.58% (without aptamers) to 33.02% (with 10th aptamer pool), and 26.01% (with NK2).10Flow Cytometry31 ± 4 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1965 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK1N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry48 ± 13 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1966 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK7N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry35 ± 9 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1967 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK8N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry107 ± 44 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1968 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK10N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry95 ± 28 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1969 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK20N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry55 ± 16 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1971 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4936–4N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay3.1 nMDetectionSELEX5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1972 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4939–280N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay1.6 nMDetectionSELEX5-naphthylmethylaminocarbonyl-dU (NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1973 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4943–51N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.8Equilibrium Binding Assay1.3 nMDetectionSELEX5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1974 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5564–89N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay9.8 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1975 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5570–54N/AN/AN/AssDNAClostridium DifficileToxin A (TcdA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay6.6 nMDetectionSELEX5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1976 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4937–55N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.76 nMDetectionSELEX5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1977 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4938–17N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.45 nMDetectionSELEX5-benzylaminocarbonyl-dU (BndU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1978 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4940–23N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.8Equilibrium Binding Assay0.07 nMDetectionSELEX5-naphthylmethylaminocarbonyl-dU (NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1979 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4944–30N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.08 nMDetectionSELEX5-tryptaminocarbonyl-dU (TrpdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1980 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5566–74N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.04 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1981 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5573–4N/AN/AN/AssDNAClostridium DifficileToxin B (TcdB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.07 nMDetectionSELEX5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1982 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers4758–6N/AN/AN/AssDNAClostridium DifficileBinary toxin, A chain (CdtA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.22 nMDetectionSELEX5-tryptaminocarbonyl-dU (TrpdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1983 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5574–49N/AN/AN/AssDNAClostridium DifficileBinary toxin, A chain (CdtA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay1.1 nMDetectionSELEX5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1984 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5579–11N/AN/AN/AssDNAClostridium DifficileBinary toxin, A chain (CdtA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay0.05 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1985 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5579–12N/AN/AN/AssDNAClostridium DifficileBinary toxin, A chain (CdtA)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.8Equilibrium Binding Assay0.69 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1986 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5556–51N/AN/AN/AssDNAClostridium DifficileBinary toxin, B chain (CdtB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay6.9 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1987 236802402013Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers5556–67N/AN/AN/AssDNAClostridium DifficileBinary toxin, B chain (CdtB)Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.N/A8Equilibrium Binding Assay4.4 nMDetectionSELEX5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1988 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4520–8PCGGCPPCGGGPACCPAPPAPCGGPPPAGCCCAGPCAGAA40N/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Staphylococcus aureus Protein A (SpA)Developed slow off-rate modified aptamer (SOMAmer) reagents against S. aureus cell surface-associated proteins, used to capture and detect S. aureus.Efficient capture of Staph. aureus over a wide range of cell densities from 5 × 10(2) CFU/ml to 5 × 10(9) CFU/ml.8Radiolabel Filter Binding Assay0·22 nMDetectionSELEXP=NapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1989 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4503–73AZCZGGZZCAAAGZGGCGAZZGGGCAZCZGGZZZZZAAGZ40N/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor A (ClfA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.Efficient capture of Staph. aureus over a wide range of cell densities from 5 × 10(2) CFU/ml to 5 × 10(9) CFU/ml.8Radiolabel Filter Binding Assay0·79 nMDetectionSELEXZ=BndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1990 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4531–56N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Staphylococcus aureus Protein A (SpA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.Able to bind whole cells of all Staph. aureus strains tested, with a detection limit of approx. 10(4) cells per well (10(5)-10(6) cells/ml).8Radiolabel Filter Binding Assay0·03 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_1991 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4522–5N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor A (ClfA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·35 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1992 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4504–27N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor B (ClfB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay1·35 nMDetectionSELEXBndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1993 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4511–67N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor B (ClfB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay3·90 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1994 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4523–79N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Clumping factor B (ClfB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·47 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1995 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4726–44N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding Protein A (FnbA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay4·38 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1996 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4745–51N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding Protein A (FnbA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·63 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1997 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4506–13N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding protein B (FnbB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay4·73 nMDetectionSELEXBndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1998 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4516–29N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding protein B (FnbB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·63 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_1999 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4527–83N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Fibronectin-binding protein B (FnbB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·84 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2000 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4727–62N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein A (IsdA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·73 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2001 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4746–3N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein A (IsdA)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·16 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2002 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4728–7N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein B (IsdB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·14 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2003 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4747–90N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein B (IsdB)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay1·98 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2004 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4507–52N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein C (IsdC)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·15 nMDetectionSELEXBndU (5-benzylaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2005 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4517–71N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein C (IsdC)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·08 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2006 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4528–22N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein C (IsdC)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay0·07 nMDetectionSELEXTrpdU (5-tryptaminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2007 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4731–69N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)Iron-regulated Surface Determinant Protein H (IsdH)Developed slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay1·30 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2008 249357142014Specific capture and detection of Staphylococcus aureus with high-affinity modified aptamers to cell surface components4730–3N/AN/AN/AssDNAStaphylococcus aureus (S. aureus) NRS384 (USA300)SasDDeveloped slow off-rate modified aptamer (SOMAmer) reagents to several Staphylococcus aureus cell surface-associated proteins via SELEX.N/A8Radiolabel Filter Binding Assay2·17 nMDetectionSELEXNapdU (5-naphthylmethyl-aminocarbonyl-dU) and 5′biotin-dA or 5′fluorescein-biotin-dA and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2010 249682392014CFP10 and ESAT6 aptamers as effective Mycobacterial antigen diagnostic reagentsCE24N/AN/A5'-GCGGAATTCTAATACGACTCACTATAGGGA ACAGTCCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.)CE (Culture filtrate protein (CFP10) and Early secreted antigenic target (ESAT6)) proteinsIdentify aptamers against CE proteins and develop an aptamer-based ELONA assay for detection in serum samples.Sensitivity and specificity using CE24 aptamer-based ELONA) were 100% and 94.1%.15Enzyme-Linked Immunosorbent Assay (ELISA)3.75 × 10(−7) MDetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_2011 249682392014CFP10 and ESAT6 aptamers as effective Mycobacterial antigen diagnostic reagentsCE15N/AN/A5'-GCGGAATTCTAATACGACTCACTATAGGGA ACAGTCCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.)CE (Culture filtrate protein (CFP10) and Early secreted antigenic target (ESAT6)) proteinsIdentify aptamers against CE proteins and develop an aptamer-based ELONA assay for detection in serum samples.Sensitivity and specificity using CE15 aptamer-based ELONA) were 89.6% and 94.1%.15Enzyme-Linked Immunosorbent Assay (ELISA)1.6 × 10(−7) MDetectionSELEXBiotinylatedN/AN/AN/AN/AN/A
ABdb_2012 244725392014DNA aptamers as a novel approach to neutralize Staphylococcus aureus α-toxinAT-27N/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAStaphylococcus aureus (S. aureus)α-toxin (exotoxin)Identify aptamers that bind to and inhibit the cytotoxic activity of a-toxin and the upregulation of inflammatory cytokines TNF-a and IL-17.Increased the viability of α-toxin-treated Jurkat T cells to about 85–90%.10N/AN/ATherapeutics/ImmunmodulatorySELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_2013 244725392014DNA aptamers as a novel approach to neutralize Staphylococcus aureus α-toxinAT-33N/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAStaphylococcus aureus (S. aureus)α-toxin (exotoxin)Identify aptamers that bind to and inhibit the cytotoxic activity of a-toxin and the upregulation of inflammatory cytokines TNF-a and IL-17.AT-33 inhibited α-toxin-induced upregulation of TNF-α and IL-17 in Jurkat T cells. Increased the viability of α-toxin-treated Jurkat T cells to about 85–90%.10N/AN/ATherapeutics/ImmunmodulatorySELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_2014 244725392014DNA aptamers as a novel approach to neutralize Staphylococcus aureus α-toxinAT-36N/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAStaphylococcus aureus (S. aureus)α-toxin (exotoxin)Identify aptamers that bind to and inhibit the cytotoxic activity of a-toxin and the upregulation of inflammatory cytokines TNF-a and IL-17.AT-36 inhibited α-toxin-induced upregulation of TNF-α and IL-17 in Jurkat T cells. Increased the viability of α-toxin-treated Jurkat T cells to about 85–90%.10N/AN/ATherapeutics/ImmunmodulatorySELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_2015 244725392014DNA aptamers as a novel approach to neutralize Staphylococcus aureus α-toxinAT-49N/AN/A5'-ACCCCTGCAGGATCCTTTGCTGGTACC-N42-AGTATCGCTAATCAGTCTAGAGGGCCCCAGAAT-3'ssDNAStaphylococcus aureus (S. aureus)α-toxin (exotoxin)Identify aptamers that bind to and inhibit the cytotoxic activity of a-toxin and the upregulation of inflammatory cytokines TNF-a and IL-17.Increased the viability of α-toxin-treated Jurkat T cells to about 85–90%.10N/AN/ATherapeutics/ImmunmodulatorySELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_2016 255111122015Development of a fluorescent enzyme-linked DNA aptamer-magnetic bead sandwich assay and portable fluorometer for sensitive and rapid listeria detectionLLO-3N/AN/A5'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAListeria Monocytogenes (ATCC 19115)Listeriolysin O (LLO) proteinA fluorescent DNA aptamer-magnetic bead sandwich assay was developed to detect LLO protein.Demonstrated LODs in the range of 4 to 61 L. monocytogenes cells or the equivalent LLO produced by 4 to 61 cells on average in a separate titration trial.8Enzyme-Linked Immunosorbent Assay (ELISA)-like Aptamer Microplate Affinity Ranking (ELASA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-BiotinylatedN/AN/AN/AN/APatent filled
ABdb_2017 271512932016Identification of ssDNA aptamers specific to clinical isolates of Streptococcus mutans strains with different cariogenicityH19N/AN/A5'-GCAATGGTACGGTACTTCC-45N-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStreptococcus MutansWhole cellIdentify a high-affinity aptamer against cariogenic S. mutans strains.Aptamer H19 showed strong binding, as indicated by higher fluorescence intensity, with Strains 12 and 17 compared with other strains.9Flow Cytometry69.45 ± 38.53 nMDetectionSubtractive SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_2018 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT9N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.Specificity and sensitivity were 95.31% and 83.00% (for 100 aPTB serum samples), 98.70% and 92.71% (for 96 aPTB sputum samples), and 94.44% and 88.71% (for 62 EPTB serum samples), respectively.13Enzyme-Linked Oligonucleotide Assay (ELONA)668 ± 159 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_2019 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT25N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.N/A13Enzyme-Linked Oligonucleotide Assay (ELONA)685 ± 131 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/AN/AN/AN/A
ABdb_2020 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT14N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.N/A13Enzyme-Linked Oligonucleotide Assay (ELONA)815 ± 144 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/AN/AN/AN/A
ABdb_2021 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT15N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.N/A13Enzyme-Linked Oligonucleotide Assay (ELONA)736 ± 207 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/AN/AN/AN/A
ABdb_2022 268503562016Generation and application of ssDNA aptamers against glycolipid antigen ManLAM of Mycobacterium tuberculosis for TB diagnosisT6N/AN/A5'-GCGGAATTCAACAGTCCGAGCC-N30-GGGTCAATGCGTCATA-3'ssDNAMycobacterium Tuberculosis (M.Tb.) Beijing genotype strainsMannose-capped lipoarabinomannan (ManLAM) epitopeIdentify an aptamer against ManLAM and develop an ELONA-based assay for the diagnosis of TB antigens using serum or sputum samples.N/A13Enzyme-Linked Oligonucleotide Assay (ELONA)998 ± 207 nMDetectionSELEXBiotinylated or FAM LabeledN/AN/AN/AN/AN/A
ABdb_2109 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA10N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.N/A15Fluorescence Binding Assay28 ± 4 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_2110 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA7N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.EA7 binds to the lipid A region of LPS and exhibits a detection limit of 3 ng/mL and a linear range of 5–250 ng/mL.15Fluorescence Binding Assay102 ± 17 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_2111 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA5N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.N/A15Fluorescence Binding Assay149 ± 30 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_2112 287280092017Bridged Rebar Graphene functionalized aptasensor for pathogenic E. coli O78:K80:H11 detectionAnti-E.coli aptamerN/AN/A5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O78:K80:H11 (MTCC 726)Whole cellA novel fabrication method of functionalised Bridged Rebar Graphene (BRG) onto EIS-based nanostructured aptasensor for label-free detection of E. coli O78:K80:H11.LOD ~ 10(1) cfu/mL with a dynamic response range from 10(1) to 10(6) cfu/mL.12Bio-Layer Interferometry (BLI)14 nMBiosensorPhenylboronic acid (PBA) mediated SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_2113 https://doi.org/10.1016/j.snb.2017.02.0982017Selection of aptamers to Neisseria meningitidis and Streptococcus pneumoniae surface specific proteins and affinity assay using thin film AlN resonatorsPA05N/AN/AN/AssDNAStreptococcus PneumoniaeSurface-specific Protein PavAGenerated SOMAmers against surface-specific proteins PavA and FHbp and developed a gravimetric aptasensor for the detection of S. pneumoniae and N. meningitidis.N/A8Enzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionSELEX5'-DIG (Digoxigenin) Labeled and 5-tryptaminocarbonyl-dU (TrpdU))N/AN/AN/AN/AN/A
ABdb_2114 https://doi.org/10.1016/j.snb.2017.02.0982017Selection of aptamers to Neisseria meningitidis and Streptococcus pneumoniae surface specific proteins and affinity assay using thin film AlN resonatorsPA08N/AN/AN/AssDNAStreptococcus PneumoniaeSurface-specific Protein PavAGenerated SOMAmers against surface-specific proteins PavA and FHbp and developed a gravimetric aptasensor for the detection of S. pneumoniae and N. meningitidis.The polyclonal population PA08 showed a frequency shift four times greater and a faster reaction rate than FH05 when measured on the gravimetric sensor.8Enzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionSELEX5'-DIG (Digoxigenin) Labeled and 5-tryptaminocarbonyl-dU (TrpdU))N/AN/ABest CandidateN/AN/A
ABdb_2115 https://doi.org/10.1016/j.snb.2017.02.0982017Selection of aptamers to Neisseria meningitidis and Streptococcus pneumoniae surface specific proteins and affinity assay using thin film AlN resonatorsFH05N/AN/AN/AssDNANeisseria MeningitidisSurface-specific Protein FHbpGenerated SOMAmers against surface-specific proteins PavA and FHbp and developed a gravimetric aptasensor for the detection of S. pneumoniae and N. meningitidis.The polyclonal population PA08 showed a frequency shift four times greater and a faster reaction rate than FH05 when measured on the gravimetric sensor.8Enzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionSELEX5'-DIG (Digoxigenin) Labeled and 5-tryptaminocarbonyl-dU (TrpdU))N/AN/ABest CandidateN/AN/A
ABdb_2116 https://doi.org/10.1016/j.snb.2017.02.0982017Selection of aptamers to Neisseria meningitidis and Streptococcus pneumoniae surface specific proteins and affinity assay using thin film AlN resonatorsFH08N/AN/AN/AssDNANeisseria MeningitidisSurface-specific Protein FHbpGenerated SOMAmers against surface-specific proteins PavA and FHbp and developed a gravimetric aptasensor for the detection of S. pneumoniae and N. meningitidis.N/A8Enzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionSELEX5'-DIG (Digoxigenin) Labeled and 5-tryptaminocarbonyl-dU (TrpdU))N/AN/AN/AN/AN/A
ABdb_2119 302453242018Aptamer functionalized MoS2-rGO nanocomposite based biosensor for the detection of Vi antigenAnti-Vi aptamers populationN/AN/A5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNASalmonella TyphiVi polysaccharide antigen (Vi antigen)Developed a novel aptamer functionalized MoS2-rGO-based electrochemical aptasensor for Vi polysaccharide antigen-mediated detection of enteric fever.Responded linearly in the range between 0.1 ng/mL to 1000 ng/mL with a detection limit of 100 pg/mL.6Saturation Binding Assay638.6 nMBiosensorSELEX5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_2124 343991932021Aptamer-superparamagnetic nanoparticles capture coupling siderophore-Fe3+ scavenging actuated with carbon dots to confer an "off-on" mechanism for the ultrasensitive detection of Helicobacter pyloriHp-1N/AN/A5'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAHelicobacter Pylori (ATCC 700392)Whole cellAptamer-modified superparamagnetic nanoparticles (SPMNPs) are used to develop a triple-module biosensor to capture H. pylori.Exhibited a wide detection range of 10-10(7) CFU/mL and a limit of detection (LOD) as low as 1 CFU/mL.10Fluorescence Spectroscopy36.91 ± 11.1 nMBiosensorSELEX5'-FAM Labeled and 5'-Amidation (NH₂)N/AN/ABest CandidateN/AN/A
ABdb_2125 343991932021Aptamer-superparamagnetic nanoparticles capture coupling siderophore-Fe3+ scavenging actuated with carbon dots to confer an "off-on" mechanism for the ultrasensitive detection of Helicobacter pyloriHp-2N/AN/A5'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAHelicobacter Pylori (ATCC 700392)Whole cellAptamer-modified superparamagnetic nanoparticles (SPMNPs) capture H. pylori.N/A10Fluorescence Spectroscopy46.31 ± 11.31 nMBiosensorSELEX5'-FAM Labeled and 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_2126 343991932021Aptamer-superparamagnetic nanoparticles capture coupling siderophore-Fe3+ scavenging actuated with carbon dots to confer an "off-on" mechanism for the ultrasensitive detection of Helicobacter pyloriHp-3N/AN/A5'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAHelicobacter Pylori (ATCC 700392)Whole cellAptamer-modified superparamagnetic nanoparticles (SPMNPs) capture H. pylori.N/A10Fluorescence Spectroscopy60.76 ± 11.69 nMBiosensorSELEX5'-FAM Labeled and 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_2127 343991932021Aptamer-superparamagnetic nanoparticles capture coupling siderophore-Fe3+ scavenging actuated with carbon dots to confer an "off-on" mechanism for the ultrasensitive detection of Helicobacter pyloriHp-4N/AN/A5'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAHelicobacter Pylori (ATCC 700392)Whole cellAptamer-modified superparamagnetic nanoparticles (SPMNPs) capture H. pylori.N/A10Fluorescence Spectroscopy41.57 ± 16.18 nMBiosensorSELEX5'-FAM Labeled and 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_2128 343991932021Aptamer-superparamagnetic nanoparticles capture coupling siderophore-Fe3+ scavenging actuated with carbon dots to confer an "off-on" mechanism for the ultrasensitive detection of Helicobacter pyloriHp-5N/AN/A5'-TGGACCTTGCGATTGCGATTGACAGC-40N-GCAGACATGAGTCTCAGGAC-3'ssDNAHelicobacter Pylori (ATCC 700392)Whole cellAptamer-modified superparamagnetic nanoparticles (SPMNPs) capture H. pylori.N/A10Fluorescence Spectroscopy67.74 ± 10.24 nMBiosensorSELEX5'-FAM Labeled and 5'-Amidation (NH₂)N/AN/AN/AN/AN/A