Method : Parallel m-SELEX (Membrane) and p-SELEX (Plate)

Total records found: 8

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_0446	24839553	2014	Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins	mA_Apt1	CACAGCGGGACAGUUUAGC-AUUAGCUAUCUUCCACGAUUCGAUCAGGCAGUACGUCGU-GGUAGGUGGGUGCGUCUAAA	78	5'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'	ssRNA	Escherichia Coli (E. Coli)	Shiga toxin 2 (Stx2)	Identify RNA aptamers against Stx1 and Stx2.	A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer mA_Apt1 (~40% cell survival).	5	Flow Cytometry	N/A	Detection	Parallel m-SELEX (Membrane) and p-SELEX (Plate)	2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 Labeled	None of the other aptamers neutralized the cytotoxic effects of Stx2.	N/A	Best Candidate	N/A	N/A
ABdb_0447	24839553	2014	Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins	mB_Apt23	CACAGCGGGACAGUUUAGC-ACAGUUAUCCGACUGCUAUUCGAUCAGGCAGUACGUAGC-GGUAGGUGGGUGCGUCUAAA	78	5'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'	ssRNA	Escherichia Coli (E. Coli)	Shiga toxin 2 (Stx2)	Identify RNA aptamers against Stx1 and Stx2.	Inhibited Stx2 binding weakly in approximately 50% of HeLa cells. A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer mB_Apt23 (~20% cell survival).	5	Flow Cytometry	N/A	Detection	Parallel m-SELEX (Membrane) and p-SELEX (Plate)	2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 Labeled	None of the other aptamers neutralized the cytotoxic effects of Stx2.	N/A	N/A	N/A	N/A
ABdb_0448	24839553	2014	Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins	mC_Apt41	CACAGCGGGACAGUUUAGC-CAGGCUGUUCUGACGCAUAAGGAAUGCGCUGUUGCAGAG-GGUAGGUGGGUGCGUCUAAA	78	5'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'	ssRNA	Escherichia Coli (E. Coli)	Shiga toxin 2 (Stx2)	Identify RNA aptamers against Stx1 and Stx2.	mC_Apt41 neutralized 0.007 pmole of Stx2 (≤60% cell survival).	5	Flow Cytometry	N/A	Detection	Parallel m-SELEX (Membrane) and p-SELEX (Plate)	2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 Labeled	None of the other aptamers neutralized the cytotoxic effects of Stx2.	N/A	N/A	N/A	N/A
ABdb_0449	24839553	2014	Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins	mD_Apt46	CACAGCGGGACAGUUUAGC-UUGGUCCUGCUUUGGAUAGUCGCGAAAGGGGUGCCACUG-GGUAGGUGGGUGCGUCUAAA	78	5'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'	ssRNA	Escherichia Coli (E. Coli)	Shiga toxin 2 (Stx2)	Identify RNA aptamers against Stx1 and Stx2.	Inhibited Stx2 binding weakly in approximately 50% of HeLa cells.	5	Flow Cytometry	N/A	Detection	Parallel m-SELEX (Membrane) and p-SELEX (Plate)	2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 Labeled	None of the other aptamers neutralized the cytotoxic effects of Stx2.	N/A	N/A	N/A	N/A
ABdb_0450	24839553	2014	Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins	pA_Apt1	CACAGCGGGACAGUUUAGC-ACAGUUAUCCGACUGCUAUUCGAUCAGGCAGUACGUAGC-GGUAGGUGGGUGCGUCUAAA	78	5'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'	ssRNA	Escherichia Coli (E. Coli)	Shiga toxin 2 (Stx2)	Identify RNA aptamers against Stx1 and Stx2.	Inhibited Stx2 binding weakly in approximately 50% of HeLa cells.	5	Flow Cytometry	N/A	Detection	Parallel m-SELEX (Membrane) and p-SELEX (Plate)	2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 Labeled	None of the other aptamers neutralized the cytotoxic effects of Stx2.	N/A	N/A	N/A	N/A
ABdb_0451	24839553	2014	Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins	pB_Apt14	CACAGCGGGACAGUUUAGC-ACCGAGCGGUUUUACGUCUCAAGUAGUAUCCCGUUUUGC-GGUAGGUGGGUGCGUCUAAA	78	5'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'	ssRNA	Escherichia Coli (E. Coli)	Shiga toxin 2 (Stx2)	Identify RNA aptamers against Stx1 and Stx2.	Did not inhibit Stx2 binding.	5	Flow Cytometry	N/A	Detection	Parallel m-SELEX (Membrane) and p-SELEX (Plate)	2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 Labeled	None of the other aptamers neutralized the cytotoxic effects of Stx2.	N/A	N/A	N/A	N/A
ABdb_0452	24839553	2014	Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins	pC_Apt22	CACAGCGGGACAGUUUAGC-AUUAGCUAUCUUCCACGAUUCGAUCAGGCAGUACGUCGU-GGUAGGUGGGUGCGUCUAAA	78	5'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'	ssRNA	Escherichia Coli (E. Coli)	Shiga toxin 2 (Stx2)	Identify RNA aptamers against Stx1 and Stx2.	A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer pC_Apt22 (≤60% cell survival).	5	Flow Cytometry	N/A	Detection	Parallel m-SELEX (Membrane) and p-SELEX (Plate)	2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 Labeled	None of the other aptamers neutralized the cytotoxic effects of Stx2.	N/A	N/A	N/A	N/A
ABdb_0453	24839553	2014	Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga Toxins	pD_Apt25	CACAGCGGGACAGUUUAGC-UUGCCAUCCUGUACUAUGCUCUAUCGGGCGGUUUAGUGAUCCUUCGUCCAACUAUC-GGUAGGUGGGUGCGUCUAAA	95	5'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'	ssRNA	Escherichia Coli (E. Coli)	Shiga toxin 2 (Stx2)	Identify RNA aptamers against Stx1 and Stx2.	Did not inhibit Stx2 binding.	5	Flow Cytometry	N/A	Detection	Parallel m-SELEX (Membrane) and p-SELEX (Plate)	2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 Labeled	None of the other aptamers neutralized the cytotoxic effects of Stx2.	N/A	N/A	N/A	N/A