AptBacterialDB

Method : Orientation selection based on Capture-SELEX

Total records found: 3

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_2109 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA10N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.N/A15Fluorescence Binding Assay28 ± 4 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_2110 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA7N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.EA7 binds to the lipid A region of LPS and exhibits a detection limit of 3 ng/mL and a linear range of 5–250 ng/mL.15Fluorescence Binding Assay102 ± 17 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_2111 https://doi.org/10.1007/s00604-017-2453-32017Orientation selection of broad-spectrum aptamers against lipopolysaccharides based on capture-SELEX by using magnetic nanoparticlesEA5N/AN/A5'-ATAGGAGTCACGACGACCAG-40N-TATGTGCGTCTACCTCTTGA-3'ssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50671)Lipopolysaccharide (LPS) lipid A domainIdentify aptamers that bind to the LPS target.N/A15Fluorescence Binding Assay149 ± 30 nMDetectionOrientation selection based on Capture-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A