AptBacterialDB

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Total records found: 633

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0072 195691562009Immediate detection of living bacteria at ultralow concentrations using a carbon nanotube based potentiometric aptasensorAptamerGGGAACAGUCCGAGCCUCACUGUUAUCCGAUAGCAGCGCGGGAUGAGGGUCAAUGCGUCAUAGGAUCCCGC71N/AssRNASalmonella Typhi (CECT 409)Type IVB Pili structural proteinsDevelop a potentiometric biosensor using an aptamer-SWCNT for selectively detecting a single CFU of ST in close to real time.Display detection range from 0.2 CFU/mL (1 CFU in 5 mL PBS) to 10(6) CFU/mL within 60sec.N/AN/AN/ABiosensorN/A3'-Amidation ((CH2)5-NH₂)N/AN/AN/AN/AN/A
ABdb_0073 195058162009A sensitive method to detect Escherichia coli based on immunomagnetic separation and real-time PCR amplification of aptamersE. coli specific RNA aptamerGGGAGAGCGGAAGCGUGCUGGGUCGCAGUUUGCGCGCGUUCCAAGUUCUCUCAUCACGGAAUACAUAACCCAGAGGUCGAU81N/AssRNAEscherichia Coli (E. Coli) DH5αWhole cellDevelop an aptamer-based, antibody-conjugated magnetic-bead sandwich assay to detect E. coli.Showed a wide dynamic range from 10¹ to 10(7) E. coli per ml and achieved a detection limit of 10 E. coli in a 1 ml sample.N/AN/AN/ABiosensorN/A2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0078 203377672010Antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from foodA8ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria Monocytogenes (F4244)Internalin A (InlA)Antibody-aptamer functionalized fibre‐optic biosensor for specific detection of Listeria monocytogenes from food products.The detection limit of this sensor was 1 × 10(3) CFU/ml, and it could successfully detect a lower inoculum size of 10(2) CFU/25g.N/AN/AN/ABiosensorN/AAlexaFluor 647 Labeled or BiotinylatedN/AN/AN/AN/AN/A
ABdb_0079 209610522010Real-time potentiometric detection of bacteria in complex samplesAptamerGGGAGAGCGGAAGCGUGCUGGGUCGCAGUUUGCGCGCGUUCCAAGUUCUCUCAUCACGGAAUACAUAACCCAGAGGUCGAU81N/AssRNAEscherichia Coli (E. Coli) CECT 675 cellsWhole cellPotentiometric aptamer-based biosensor to detect E. coli CECT 675 as a nonpathogenic surrogate for pathogenic E. coli O157:H7 in complex liquid samples.Display LOD of 10(4) CFU/mL with a linear range from 4 to ∼10(4) CFU/mL. The lowest bacterial count detected in complex matrices was 12 CFU in 2 mL of milk (6 CFU/mL) and 26 CFU in 1 mL of apple juice.N/AN/AN/ABiosensorN/A3'-Amidation ((CH2)5-NH₂)N/AN/AN/AN/AN/A
ABdb_0123 218751072011Identification of environmental reservoirs of nontyphoidal salmonellosis: aptamer-assisted bioconcentration and subsequent detection of salmonella typhimurium by quantitative polymerase chain reactionSal aptamerCTCACCAGGAGATTACAACATGG-TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG-AGCTCAGACCAAAAGTGACCATC865'-CTCACCAGGAGATTACAACATGG-N40-AGCTCAGACCAAAAGTGACCATC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDetection of Salmonella Typhimurium by serovar-specific DNA aptamer-conjugated dynabeads in water samples.Sediments from the selected sampling locations also exhibit Salmonella Typhimurium in the range of 3.37 × 10(4) to 3.08 × 10(9) cfu/100 g.N/AN/AN/ADetectionN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0136 231906952012Aptasensors for rapid detection of Escherichia coli O157:H7 and Salmonella typhimuriumA1CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7 (CICC 21530)Whole cellAptasensors based on label-free aptamers and gold nanoparticles (AuNPs) for the detection of E. coli O157:H7 and Salmonella typhimurium.Detects as low as 10(5) CFU/ml of target bacteria within 20 min or less, with 100% specificity.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0137 231906952012Aptasensors for rapid detection of Escherichia coli O157:H7 and Salmonella typhimuriumA2CCAAAGGCTACGCGTTAACGTGGTGTTGG29N/AssDNASalmonella Typhimurium (S. Typhimurium) (CMCC 50115)Whole cellAptasensors based on label-free aptamers and gold nanoparticles (AuNPs) for the detection of E. coli O157:H7 and Salmonella typhimurium.Detects as low as 10(5) CFU/ml of target bacteria within 20 min or less, with 100% specificity.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0139 221541692012Label-free detection of Staphylococcus aureus in skin using real-time potentiometric biosensors based on carbon nanotubes and aptamersNH2-AptamerGCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA single-walled carbon nanotube (SWCNT) functionalized with an aptamer-based biosensor to detect Staphylococcus aureus in real-time.With covalent functionalization, the minimum concentration detected was 8 × 10(2) CFU/mL; with the non-covalent approach, it was 10(7) CFU/mL.N/AN/AN/ADetectionN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0140 221541692012Label-free detection of Staphylococcus aureus in skin using real-time potentiometric biosensors based on carbon nanotubes and aptamersPyr-AptamerGCAATGGTACGGTACTTCCGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTATCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA single-walled carbon nanotube (SWCNT) functionalized with an aptamer-based biosensor to detect Staphylococcus aureus in real-time.With covalent functionalization, the minimum concentration detected was 8 × 10(2) CFU/mL; with the non-covalent approach, it was 10(7) CFU/mL.N/AN/AN/ADetectionN/A3'-C3-PyrN/AN/AN/AN/AN/A
ABdb_0141 224445662012Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella Typhimurium and Staphylococcus aureusAptamer1TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50761)Whole cellDeveloped a luminescent bioassay for the simultaneous detection of S. Typhimurium and S. aureus using aptamer-conjugated MNPs and UCNPs (NaY0.78F4:Yb0.2, Tm0.02 UCNPs modified aptamer 1 and NaY0.28F4:Yb0.70, Er0.02 UCNPs modified aptamer 2).Display linear range of 10(1)-10(5) cfu/mL with a limit of detection of 5 cfu/mL for S. Typhimurium.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0142 224445662012Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella Typhimurium and Staphylococcus aureusAptamer2GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a luminescent bioassay for the simultaneous detection of S. Typhimurium and S. aureus using aptamer-conjugated MNPs and UCNPs (NaY0.78F4:Yb0.2, Tm0.02 UCNPs modified aptamer 1 and NaY0.28F4:Yb0.70, Er0.02 UCNPs modified aptamer 2).Display linear range of 10(1)-10(5) cfu/mL with a limit of detection of 8 cfu/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0143 231450452012An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7E17F-72ATCCGTCACACCTGCTCTATCAAATGTGCAGATATCAAGACGATTTGTACAAGATGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O157:H7Lipopolysaccharide (LPS)Develop a colorimetric aptasensor employing PDA vesicles for the determination of E. coli O157:H7.N/AN/AN/AN/ABiosensorN/A5'-Amidation (NH₂) and FITC LabeledN/AN/AN/AN/AN/A
ABdb_0144 231450452012An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7E17F-37ATCAAATGTGCAGATATCAAGACGATTTGTACAAGAT37N/AssDNAEscherichia Coli (E. Coli) O157:H7Lipopolysaccharide (LPS)Develop a colorimetric aptasensor employing PDA vesicles for the determination of E. coli O157:H7.Could detect cellular concentrations in a range of 10^4 to 10^8 CFU/ml within 2 hours with a detection limit of 10(4) CFU/ml.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) and FITC LabeledN/AN/AN/AN/AN/A
ABdb_0145 231450452012An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7E18R-72ATACGGGAGCCAACACCATTCTATCGTTCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGGAT72N/AssDNAEscherichia Coli (E. Coli) O157:H7Lipopolysaccharide (LPS)Develop a colorimetric aptasensor employing PDA vesicles for the determination of E. coli O157:H7.N/AN/AN/AN/ABiosensorN/A5'-Amidation (NH₂) and FITC LabeledN/AN/AN/AN/AN/A
ABdb_0146 231450452012An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7E18R-42CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Lipopolysaccharide (LPS)Develop a colorimetric aptasensor employing PDA vesicles for the determination of E. coli O157:H7.Could detect cellular concentrations in a range of 10^4 to 10^8 CFU/ml within 2 hours with a detection limit of 10(4) CFU/ml.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) and FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0186 https://doi.org/10.1002/elan.2011007002012A Rapid and Sensitive Aptamer-Based Electrochemical Biosensor for Direct Detection of Escherichia Coli O111L9FATCCGTCACACCTGCTCTACTGGCCGGCTCAGCATGACTAAGAAGGAAGTTATGTGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O111Lipopolysaccharide (LPS)Developed an electrochemical biosensor based on target-induced aptamer displacement for the detection of E. coli O111.Achieved a detection limit of 112 CFU/mL in PBS and 305 CFU/mL in milk within 3.5 hours.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0321 https:doi.org10.1007s13765-013-3019-72013Potential of fluorophore labeled aptamers for Pseudomonas aeruginosa detection in drinking waterP.aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 10145)Whole cellDevelop a fluorophore-labelled aptamer to detect P. aeruginosa in drinking water.The limit of detection for P. aeruginosa was 5.07 cells/mL, with a linear dynamic range of 5.64 to 100 cells/mL.N/AN/AN/ABiosensorN/AFITC LabeledN/AN/AN/AN/AN/A
ABdb_0333 238720602013Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteriaA1GGGAACAGUCCGAGCCUCACUGUUAUCCGAUAGCAGCGCGGGAUGAGGGUCAAUGCGUCAUAGGAUCCCGC71N/AssRNASalmonella Typhi (CECT 409)Type IVB Pili structural proteinsDevelop a potentiometric biosensor based on carbon nanotubes (transducer layer) and aptamers (sensing layer) for the detection of bacteria.Display linear range from 0.2 CFU/mL (1 CFU in 5 mL PBS) to 10(6) CFU/mL of ST and LOD of 0.2 CFU/mL.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0334 238720602013Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteriaA2GGGAGAGCGGAAGCGUGCUGGGUCGCAGUUUGCGCGCGUUCCAAGUUCUCUCAUCACGGAAUACAUAACCCAGAGGUCGAU81N/AssRNAEscherichia Coli (E. Coli) (CECT 675)Type IVB Pili structural proteinsDevelop a potentiometric biosensor based on carbon nanotubes (transducer layer) and aptamers (sensing layer) for the detection of bacteria.Display linear range from 4 to 10(4) CFU/mL and LOD of 4 CFU/mL, and the signal was stable after 120 s.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0335 238720602013Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteriaA3GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CECT 4630)Type IVB Pili structural proteinsDevelop a potentiometric biosensor based on carbon nanotubes (transducer layer) and aptamers (sensing layer) for the detection of bacteria.Display linear range from 8 × 10(2) CFU/mL–10(8) CFU/mL with LOD of 8 x10(2) CFU/mL and the signal was stable for at least one hour.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0338 239293942013A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detectionL. acidophilus (FALA)ATCCGTCACACCTGCTCTACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTATGGTGTTGGCTCCCGTAT73N/AssDNALactobacillum Acidophilius (ATCC 4356)Whole cellDeveloped an aptamer-functionalized graphene oxide (GO) nano-biosensor for multiplexed pathogen detection.Detection limits (LOD) of 11.0 cfu/mL (L. acidophilus).N/AN/A13 nMBiosensorN/A5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0339 239293942013A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detectionS. aureus (FASA)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped an aptamer-functionalized graphene oxide (GO) nano-biosensor for multiplexed pathogen detection.Detection limits (LOD) of 800.0 cfu/mL (S. aureus) and linear ranges of 10(4)–10(6) cfu/mL.N/AN/A35 nMBiosensorN/A5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0340 239293942013A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detectionS. enterica (FASE)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-functionalized graphene oxide (GO) nano-biosensor for multiplexed pathogen detection.Detection limits (LOD) of 61.0 cfu/mL (S. enterica) and linear ranges of 42.2–675.0 cfu/mL.N/AN/AN/ABiosensorN/A5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0341 https://doi.org/10.1016/j.snb.2013.01.0622013A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteinsECA IGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCGC37N/AssDNAEscherichia Coli (E. Coli) (ATCC® 8739™)Outer membrane protein (OMP)Developed a label-free aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins (OMPs).Demonstrated in a dynamic detection range of 1 × 10(-7)–2 × 10(-6) M.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0342 https://doi.org/10.1016/j.snb.2013.01.0622013A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteinsECA IIACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA37N/AssDNAEscherichia Coli (E. Coli) (ATCC® 8739™)Outer membrane protein (OMP)Developed a label-free aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins (OMPs).Demonstrated in a dynamic detection range of 1 × 10(-7)–2 × 10(-6) M.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0413 244451152014An aptamer-based electrochemical biosensor for the detection of SalmonellaAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellAptamer-based electrochemical biosensor for Salmonella detection.A detection limit of 3 cfu/mL was achieved, with a linear range of 2.4 cfu/mL to 2.4 × 10^3 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0414 243259832014Graphene-based potentiometric biosensor for the immediate detection of living bacteriaSA20GCAATGGTACGGTACTTCCGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTATCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CECT 4630)Whole cellDeveloped a potentiometric aptasensor based on graphene oxide (GO-covalently conjugated aptamer) and reduced graphene oxide (RGO-non-covalently conjugated aptamer) to detect S. aureus.Able to detect 1 CFU/mL in a few minutes.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0415 243259832014Graphene-based potentiometric biosensor for the immediate detection of living bacteriaSA31GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CECT 4630)Whole cellDeveloped a potentiometric aptasensor based on graphene oxide (GO-covalently conjugated aptamer) and reduced graphene oxide (RGO-non-covalently conjugated aptamer) to detect S. aureus.Able to detect 1 CFU/mL in a few minutes.N/AN/AN/ABiosensorN/A3'-C3-PyrN/AN/AN/AN/AN/A
ABdb_0416 248045562014Aptamer-fluorescent silica nanoparticles bioconjugates based dual-color flow cytometry for specific detection of Staphylococcus aureusAptamerGCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDevelop aptamer recognition and FSiNPs label-based dual-colour flow cytometry assay (Aptamer/FSiNPs-DCFCM) for the detection of S. aureus.Detects as low as 1.5×10(2) and 7.6×10(2) cells/mL S. aureus in buffer and spiked milk, respectively.N/AN/AN/ABiosensorN/A5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0427 242800492014Aptamer cocktails: enhancement of sensing signals compared to single use of aptamers for detection of bacteriaE1GCAATGGTACGGTACTTCCACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (KCTC 2571)Surface proteinDeveloped an aptamer cocktail with different components on the cell surface to enhance the sensing signal for bacterial detection.In electrochemical detection, the cocktail achieved an approximately 3.7 × 10^2 CFU/mL LOD (18-fold lower than the average single aptamer).N/AN/A12.4 nMBiosensorN/A5'-FAM Labeled and 3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0428 242800492014Aptamer cocktails: enhancement of sensing signals compared to single use of aptamers for detection of bacteriaE2GCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (KCTC 2571)Surface proteinDeveloped an aptamer cocktail with different components on the cell surface to enhance the sensing signal for bacterial detection.In electrochemical detection, the cocktail achieved an approximately 3.7 × 10^2 CFU/mL LOD (18-fold lower than the average single aptamer).N/AN/A25.2 nMBiosensorN/A5'-FAM Labeled and 3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0429 242800492014Aptamer cocktails: enhancement of sensing signals compared to single use of aptamers for detection of bacteriaE10GCAATGGTACGGTACTTCCGTTGCACTGTGCGGCCGAGCTGCCCCCTGGTTTGTGAATACCCTGGGCAAAAGTGCACGCTACTTTGCTAA90N/AssDNAEscherichia Coli (E. Coli) (KCTC 2571)Surface proteinDeveloped an aptamer cocktail with different components on the cell surface to enhance the sensing signal for bacterial detection.In electrochemical detection, the cocktail achieved an approximately 3.7 × 10^2 CFU/mL LOD (18-fold lower than the average single aptamer).N/AN/A14.2 nMBiosensorN/A5'-FAM Labeled and 3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0430 245686252014Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labelsApt₁GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a multiplex method for the simultaneous detection of three pathogenic bacteria using multicolour upconversion nanoparticles (UCNPs) coupled with aptamers.The linear range showed from 50-10(6) cfu/mL, and the limits of detection were 25 cfu/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0431 245686252014Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labelsApt₂ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a multiplex method for the simultaneous detection of three pathogenic bacteria using multicolour upconversion nanoparticles (UCNPs) coupled with aptamers.The linear range showed from 50-10(6) cfu/mL, and the limits of detection were 10 cfu/mL for V. parahemolyticus.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0432 245686252014Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labelsApt₃ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a multiplex method for the simultaneous detection of three pathogenic bacteria using multicolour upconversion nanoparticles (UCNPs) coupled with aptamers.The linear range showed from 50-10(6) cfu/mL, and the limits of detection were 15 cfu/mL for S. typhimurium.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0433 246505832014A universal fluorescent aptasensor based on AccuBlue dye for the detection of pathogenic bacteriaST-aptAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA universal fluorescent aptasensor based on the AccuBlue dye was developed for the detection of pathogenic bacteria.Exhibit a linear concentration range of 50 to 10(6) cfu/mL, and the LOD was 25 cfu/mL in 1.5 h for S. typhimurium.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0434 246505832014A universal fluorescent aptasensor based on AccuBlue dye for the detection of pathogenic bacteriaVP-aptTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellAptamer is hybridized with complementary DNA (cDNA) to form fluorescent dsDNA.Exhibit a linear concentration range of 50 to 10(6) cfu/mL, and the LOD was 35 cfu/mL in 1.5 h for V. parahaemolyticus.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0445 252201632014Potentiometric Aptasensing of Listeria monocytogenes Using Protamine as an IndicatorLM aptamerATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria MonocytogenesInternalin A (InlA)Develop a label-free potentiometric aptasensor for selective detection of L. monocytogenes.LM can be detected down to 10 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0454 https://doi.org/10.1016/j.foodcont.2013.09.0462014A visual detection method for Salmonella Typhimurium based on aptamer recognition and nanogold labelingAptamer 1TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (ATCC 50761)Whole cellDeveloped a sandwich detection method based on the recognition of aptamers coupled with nanogold labelling and silver signal amplification for the detection of Salmonella Typhimurium.Obtained a linear range of 10-10(6) cfu/mL, and the detection limit was observed to be 7 cfu/mL.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0455 https://doi.org/10.1016/j.foodcont.2013.09.0462014A visual detection method for Salmonella Typhimurium based on aptamer recognition and nanogold labelingAptamer 2TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (ATCC 50761)Whole cellDeveloped a sandwich detection method based on the recognition of aptamers coupled with nanogold labelling and silver signal amplification for the detection of Salmonella Typhimurium.Obtained a linear range of 10-10(6) cfu/mL, and the detection limit was observed to be 7 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0456 https://doi.org/10.1007/s00604-014-1170-42014Fluorescent aptasensor for the determination of Salmonella typhimurium based on a graphene oxide platformAptamerGGGAGCTCAGAATAAACGCTCAAGGGCAGGTGTTATGTGTACTGCTACAGTGTGGTTGTTCGACATGAGGCCCGGAC77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (CMCC 50115)Whole cellDeveloped a fluorescent aptasensor based on a graphene oxide platform for the determination of S. typhimurium.Displays a linear response to bacteria in the concentration range from 1 × 10(3) to 1 × 10(8) CFU/mL, with a detection limit of 100 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0457 249138712014A sensitive gold nanoparticle-based colorimetric aptasensor for Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a gold nanoparticle-based colorimetric aptasensor for S. aureus using tyramine signal amplification (TSA) technology.Exhibited a linear detection range from 10 to 10⁶ cfu/mL with a limit of detection (LOD) of 9 cfu/mL.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0458 251883922014Gold nanoparticle-based enzyme-linked antibody-aptamer sandwich assay for detection of Salmonella TyphimuriumSTM-binding aptamerATCCGTCACACCTGCTCTGGAGCAATATGGTGGAGAAACGTGGTGTTGGCTCCCGTAT58N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (CMCC 50115)Whole cellDevelop a gold nanoparticle-based enzyme-linked antibody-aptamer sandwich (nano-ELAAS) method for quantitative detection of STM.Quantitative detection range: 1 × 10³ to 1 × 10⁸ CFU/mL and LOD: 1 × 10³ CFU/mL, and a selectivity of >10-fold for STM in samples containing other bacteria at higher concentration, with an assay time of less than 3 h.N/ALPS-Aptamer Plate Binding Assay19.59 ± 0.35 nMBiosensorN/A5'-Amidation (NH₂-(CH2)6) and 5'-FITC Labeled and 5'-BiotinylatedN/AMMP-aptamers and nanoprobes were stored at 4°C for 2 weeks, no obvious change of the S/B was observed.N/AN/AN/A
ABdb_0459 254378032014Application of DNA Aptamers and Quantum Dots to Lateral Flow Test Strips for Detection of Foodborne Pathogens with Improved Sensitivity versus Colloidal GoldEcO 3RCACACCTGCTCTGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCGTGGTGTTGGCTC60N/AssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain) and Escherichia Coli (E. Coli) O157:H7Whole cellSandwich (EcO 3R/4F) Lateral Flow Assay (aptamer-Qdot LF strip) for the detection of E.coli.Sandwich combination (EcO 3R-conjugate and EcO 4F-capture aptamer) yielded a visible limit of detection (LOD) of ~3,000 E. coli 8739 and ~6,000 E. coli O157:H7 in buffer.N/AN/AN/ABiosensorN/A5'-Biotinylated and 3′-DIG (Digoxigenin) LabeledN/AN/AN/AN/AU.S. Patent Application 13/136,820
ABdb_0460 254378032014Application of DNA Aptamers and Quantum Dots to Lateral Flow Test Strips for Detection of Foodborne Pathogens with Improved Sensitivity versus Colloidal GoldEcO 4FATACGGGAGCCAACACCATAATATGCCGTAAGGAGAGGCCTGTTGGGAGCGCCGTAGAGCAGGTGTGACGGAT73N/AssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain) and Escherichia Coli (E. Coli) O157:H7Whole cellSandwich Lateral Flow Assay (aptamer-Qdot LF strip) for the detection of E.coli.Sandwich combination (EcO 3R-conjugate and EcO 4F-capture aptamer) yielded a visible limit of detection (LOD) of ~3,000 E. coli 8739 and ~6,000 E. coli O157:H7 in buffer.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AU.S. Patent Application 13/136,820
ABdb_0461 https://doi.org/10.1007/s00604-014-1406-32014Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles.Apt1 ( V. parahaemolyticus aptamer)ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDevelop a dual fluorescence resonance energy transfer (FRET) from gQDs and rQDs and amorphous carbon nanoparticles (CNPs) for simultaneous detection of V. parahaemolyticus and S. typhimurium.LOD: 25 cfu/mL and linear range from 50 to 10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0462 https://doi.org/10.1007/s00604-014-1406-32014Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles.Apt2 (S. typhimurium aptamer)ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028) (ATCC 14028)Whole cellDevelop a dual fluorescence resonance energy transfer (FRET) from gQDs and rQDs and amorphous carbon nanoparticles (CNPs) for simultaneous detection of V. parahaemolyticus and S. typhimurium.LOD: 35 cfu/mL and linear range from 50 to 10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0466 244919612014Lateral flow biosensor for DNA extraction-free detection of Salmonella based on aptamer mediated strand displacement amplificationCapture-aptamer (c-aptamer)TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCCGAACAAGCTGAGGATGAAGAACAACGGCT89N/AssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (OMP)The gold-nanoparticle-based lateral flow biosensor was developed for the rapid detection of S. enteritidis.As low as 10(1) CFU of S. enteritidis per mL was detected.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0467 244919612014Lateral flow biosensor for DNA extraction-free detection of Salmonella based on aptamer mediated strand displacement amplificationAmplify-aptamer (a-aptamer)TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCCGAACAAGCTGAGGATGAAGAACAACGGCT89N/AssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (OMP)The gold-nanoparticle-based lateral flow biosensor was developed for the rapid detection of S. enteritidis.As low as 10(1) CFU of S. enteritidis per mL was detected.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0499 242874072014Aptamer biosensor for sensitive detection of toxin A of Clostridium difficile using gold nanoparticles synthesized by Bacillus stearothermophilusAptamerTAGTGATGCCTTTGTTGAGA-AGACTTGGGGGCAGGGTCGTGAGGACGTACGTGCGAGTGT-TCTTCATCGTCCACTAAATT805'-TAGTGATGCCTTTGTTGAGA-N40-TCTTCATCGTCCACTAAATT-3'ssDNAClostridium DifficileToxin A (TOA)Developed an electrochemical biosensor to detect TOA based on Nafion-thionine-GNPS-modified screen-printed electrode (SPE).Shows a linear range of 0–200 ng/mL with the detection limit of 1 nM for TOA.N/AN/AN/ABiosensorN/A5'-FITC LabeledN/A87.6% of its original response remaining after storage for 2 weeks at 4°C in PBS (pH 7.0).N/AN/AN/A
ABdb_0500 250162532014Evanescent wave DNA-aptamer biosensor based on long period gratings for the specific recognition of E. coli outer membrane proteinsAptamerGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCG36N/AssDNAEscherichia Coli (E. Coli) (ATCC® 8739™)Outer membrane protein (OMP)A label-free remote evanescent wave fibre optic sensor using long-period gratings (LPGs) was developed for the detection of EcOMPs.Displayed linear responses in the range of 0.1 nM to 10 nM EcOMPs and LOD of 10 nM in less than 1 h.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0501 https://doi.org/10.1039/C4RA01901F2014A simple aptamer biosensor for Salmonellae enteritidis based on fluorescence-switch signaling graphene oxideS-aptamerTCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCC60N/AssDNASalmonella Enteritidis (S. Enteritidis) (ATCC 13076)Whole cellDeveloped a fluorescent aptasensor with an aptamer functionalized into graphene oxide to detect S. enteritidis.Can detect as low as 40 CFU/mL of S. enteritidis in 30 min.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0540 258914722015Antibiotic loaded nanocapsules functionalized with aptamer gates for targeted destruction of pathogenSA20hpGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAptamer-gated nanocapsules for the specific targeting of vancomycin to bacteria for the controlled release of vancomycin.15-fold increase of efficacy for vancomycin with MIC of 0.420 μg/mL.N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-AlexaFluor 488 Labeled and 3'-(PEG)-AGGGCGC-BH1MICs of vancomycin-nanoparticles for S. epidermidis (control) were found to be 6.295 µg/mL.N/AN/AN/AN/A
ABdb_0672 254762862015Aptamer-functionalized localized surface plasmon resonance sensor for the multiplexed detection of different bacterial speciesLac-aptAGCAGCACAGAGGTCAGATGTAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGTGCCTATGCGTGCTACCGTGAA78N/AssDNALactobacillus Acidophilus (KCTC 3164)Whole cellThe aptamer-immobilized LSPR sensor was developed for bacteria detection.Showed a detection limit of 30 cfu per assay.N/AN/A13 ± 3 nMBiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0673 254762862015Aptamer-functionalized localized surface plasmon resonance sensor for the multiplexed detection of different bacterial speciesSty-aptTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 2421)Whole cellThe aptamer-immobilized LSPR sensor was developed for bacteria detection.Showed a detection limit of 30 cfu per assay.N/AN/AN/ABiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0674 254762862015Aptamer-functionalized localized surface plasmon resonance sensor for the multiplexed detection of different bacterial speciesPae-aptCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 15692)Whole cellThe aptamer-immobilized LSPR sensor was developed for bacteria detection.Showed a detection limit of 30 cfu per assay.N/AN/A17.27 ± 5 nMBiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0675 https://doi.org/10.1007/s00604-015-1649-72015Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubesAnti-Salmonella aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50761)Whole cellDevelop a label-free impedimetric aptasensor based on an amino-modified aptamer bound to rGO-MWCNT composite for the detection of Salmonella.Exhibits a linear range from 75 to 7.5 × 10(5) cfu/mL and a detection limit of 25 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0676 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P (Parental)AGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.5′-α-Gal, 3′-FAM α20A24P significantly increased phagocytosis in the presence of either transgenic mouse IgG or hIVIG.N/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM Labeled, 5′-α-Gal or Biotin-TEG modifiedN/AN/AN/AN/AN/A
ABdb_0677 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A2AGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCT69N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0678 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A3AGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCC52N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.5′-α-Gal α20A24P.A3 significantly increased phagocytosis in the presence of either transgenic mouse IgG or human IVIG.N/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM Labeled and 5′-α-GalN/AN/AN/AN/AN/A
ABdb_0679 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A4AGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCA53N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0680 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A5AGGTCAGATGGGGGGAAGACAC22N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0681 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A6AAGGCCGGGGTGAAGTGTAGAGGCCTA27N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0682 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A8TGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGC43N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0683 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A9AGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTAT57N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0684 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer15A3PTTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGGCATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0685 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A1PTTCACGGTAGCACGCATAGGCAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCCCATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0686 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A8PAGCAGCACAGAGGTCAGATGCCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCCCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0687 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A8CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC40N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0688 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A9PAGCAGCACAGAGGTCAGATGCACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGGCCTATGCGTGCTACCGTGAA79N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0689 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A9CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG39N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0690 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A12PTTCACGGTAGCACGCATAGGGCCCGACACTCGTCCACCCGATACCTCTCATGTGTCCCCATCTGACCTCTGTGCTGCT78N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0691 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A14PAGCAGCACAGAGGTCAGATGGGCATGGGGAAGAGAAAGCGGGATAACTTCGTTACCGGGCCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0692 263022562015Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticusApt 1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a colorimetric aptasensor system based on sandwich-type complex (AuNPs−HRP−aptamer−target−aptamer−MNPs) to detect V. parahemolyticus.Linear detection range: 10 to 10^6 cfu/mL, with a limit of detection (LOD) of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0693 263022562015Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticusApt 2TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a colorimetric aptasensor system based on sandwich-type complex (AuNPs−HRP−aptamer−target−aptamer−MNPs) to detect V. parahemolyticus.Linear detection range: 10 to 10^6 cfu/mL, with a limit of detection (LOD) of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0694 257022752015A sensitive lateral flow biosensor for Escherichia coli O157:H7 detection based on aptamer mediated strand displacement amplificationAmplification-aptamer (a-aptamer)ATCAAATGTGCAGATATCAAGACGATTTGTACAAGATCCATGCTGAGGT49N/AssDNAEscherichia Coli (E. Coli) O157:H7Outer membrane protein (OMP)Developed a Lateral Flow Biosensor (LFB) with gold nanoparticle (AuNP) probes for the detection of E.coli.Can detect as low as 10 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0695 257022752015A sensitive lateral flow biosensor for Escherichia coli O157:H7 detection based on aptamer mediated strand displacement amplificationCapture-aptamer (c-aptamer)TTTCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG45N/AssDNAEscherichia Coli (E. Coli) O157:H7Outer membrane protein (OMP)Developed a Lateral Flow Biosensor (LFB) with gold nanoparticle (AuNP) probes for the detection of E.coli.Can detect as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0696 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA20GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 70.86 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0697 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA23GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 61.50 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0698 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA34GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 72.42 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0699 254611752015A new aptamer/graphene interdigitated gold electrode piezoelectric sensor for rapid and specific detection of Staphylococcus aureusS. aureus aptamer.GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped an aptamer/graphene interdigitated gold electrode piezoelectric sensor to detect Staphylococcus aureus.Achieved a detection limit of 41 cfu/mL with a linear range of 4.1×10(1) to 4.1×10(5) cfu/mL. The detection is rapid, completing in less than 60 minutes.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0700 262417352015Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellA GNPs-modified Raman-based biosensor was developed for the simultaneous detection of S. typhimurium and S. aureus.Linear detection range of 10^2 to 10^7 cfu/mL for both; LOD of 15 cfu/mL for S. typhimurium.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0701 262417352015Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA GNPs-modified Raman-based biosensor was developed for the simultaneous detection of S. typhimurium and S. aureus.Linear detection range of 10^2 to 10^7 cfu/mL for both; LOD of 35 cfu/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0702 254675002015Pathogen detection in complex samples by quartz crystal microbalance sensor coupled to aptamer functionalized core-shell type magnetic separationAptamerCTGATGTGTGGGTAGGTGTCGTTGATTTCTTCTGGTGGGG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an aptamer-based QCM biosensor for the determination of Salmonella in food samples.Showed specific detection of Salmonella cells at 100 CFU/mL from a milk sample and a linear response range from 100 to 4 × 10(4) CFU/mL cells.N/AQuartz Crystal Microbalance (QCM)3259 CFU mL−1BiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0703 https://doi.org/10.1080/00032719.2015.10362782015Aptamer Immobilized Magnetoelastic Sensor for the Determination of Staphylococcus aureusS. aureus aptamer.GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDevelop an aptamer-based magnetoelastic sensor for the detection of S. aureus.Linear dynamic range of 10^1–10^11 cfu/mL and detection limit of 5 cfu/mL.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_0704 https://doi.org/10.1039/C4AY02880E2015Rapid and sensitive detection of Salmonella typhimurium using aptamer-conjugated carbon dots as fluorescence probeS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop a fluorescence probe based on amino-modified aptamer conjugated to carboxyl-modified carbon dots (CDs) for detection of Salmonella typhimurium.Limit of Detection (LOD): 50 cfu mL⁻¹ and linear range of 10(3)-10(5) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0705 https://doi.org/10.1039/C4AY02662D2015A chronopotentiometric flow injection system for aptasensing of E. coli O157EcO 4 RevACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA37N/AssDNAEscherichia Coli (E. Coli) O157 (ATCC 35150)Whole cellDevelop a potentiometric aptasensor employing simple flow injection analysis system for the detection of E. coli O157.N/AN/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0706 https://doi.org/10.1039/C4AY02662D2015A chronopotentiometric flow injection system for aptasensing of E. coli O157EcO 3 RevGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCG36N/AssDNAEscherichia Coli (E. Coli) O157 (ATCC 35150)Whole cellDevelop a potentiometric aptasensor employing simple flow injection analysis system for the detection of E. coli O157.Exhibited a linear range of 10-10(4) cfu/mL and the detection limit of 10 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0707 https://doi.org/10.1039/C4AY02662D2015A chronopotentiometric flow injection system for aptasensing of E. coli O157E17F-37ATCAAATGTGCAGATATCAAGACGATTTGTACAAGAT37N/AssDNAEscherichia Coli (E. Coli) O157 (ATCC 35150)Whole cellDevelop a potentiometric aptasensor employing simple flow injection analysis system for the detection of E. coli O157.N/AN/AN/AN/ABiosensorN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_0708 https://doi.org/10.1039/C4AY02662D2015A chronopotentiometric flow injection system for aptasensing of E. coli O157E18R-42CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157 (ATCC 35150)Whole cellDevelop a potentiometric aptasensor employing simple flow injection analysis system for the detection of E. coli O157.N/AN/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0709 https://doi.org/10.1016/j.lwt.2015.07.0212015Identification and characteristics of aptamers against inactivated Vibrio alginolyticusAptamer #7TCAGTCGCTTCGCCGTCTCCTTC-GGGGGCGCGGTGAGGGGCTGCACAAGAGGGAG-GCACAAGAGGGAGACCCCAGAGGG79N/AssDNAVibrio AlginolyticusWhole cellDevelop an aptamer detection assay for inactivated V. alginolyticus.The detection limit for aptamer #7 for inactivated V. alginolyticus was 10 cells/ml.N/AFluorescence Spectroscopy28.39 ± 11.46 nMDetectionN/A5'-DIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0710 https://doi.org/10.1016/j.lwt.2015.07.0212015Identification and characteristics of aptamers against inactivated Vibrio alginolyticusAptamer #8TCAGTCGCTTCGCCGTCTCCTTC-AGCCGGGGTGGTCAGTAGGAGCAGCACAAGAGGGA-GCACAAGAGGGAGACCCCAGAGGG82N/AssDNAVibrio AlginolyticusWhole cellDevelop an aptamer detection assay for inactivated V. alginolyticus.The detection limit for aptamer #8 for inactivated V. alginolyticus was 10 cells/ml.N/AFluorescence Spectroscopy12.82 ± 6.00 nMDetectionN/A5'-DIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0711 https://doi.org/10.1016/j.lwt.2015.07.0212015Identification and characteristics of aptamers against inactivated Vibrio alginolyticusAptamer #23TCAGTCGCTTCGCCGTCTCCTTC-GTAGGAGGTAGTCGGAGAGGCGAATGAGAGGGGAAGCACAAGAGGGA-GCACAAGAGGGAGACCCCAGAGGG94N/AssDNAVibrio AlginolyticusWhole cellDevelop an aptamer detection assay for inactivated V. alginolyticus.The detection limit for aptamer #23 for inactivated V. alginolyticus was 10(3) cells/ml.N/AFluorescence Spectroscopy46.89 ± 15.87 nMDetectionN/A5'-DIG (Digoxigenin) LabeledN/AN/AN/AN/AN/A
ABdb_0712 https://doi.org/10.1080/00032719.2015.10529742015Determination of Shigella flexneri by a Novel Fluorescent AptasensorAptamer1CCTCATGTCGAACAGCAACACTGCAACACTGTATAGTCCTGTGTGCCTTGAGCGTTTATTCTGAGCT67N/AssDNAShigella Flexneri (CGMCC 1.1868)Whole cellDeveloped a homogeneous fluorescent aptasensor using a dye-labelled aptamer and graphene oxide, using target recycling amplification for Shigella flexneri detection.A linear relationship was displayed from 500 to 10(9) CFU/mL with a limit of detection of 100 CFU/mL.N/AN/A29 ± 4 nMBiosensorN/ACarboxyfluorescein-GGGCCC at the 5' and 3' endsN/AN/AN/AN/AN/A
ABdb_0722 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 10GATGATGGACGTATATCGTCTCCCATGAATTCAGTCGGACAGCG44N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0723 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 22ATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGCG41N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0724 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 45ATGGACGAATATCGTCTCCCAGTGAATTCAGTCGGACAGC40N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0725 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 60CGCCCACCCATAATGGATCAGGGCGGGCACCACGATG37N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0726 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 1CGAAGGGGCTATGCCGCCTACATAGACCGTCACGA35N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0727 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 2GGCCGGCAATACGGCCGAGCCCGGGGTTCCTCCGA35N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0728 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 3GCCACGCGCAGCAATCAAACCCGGCCCCCTGCTCC35N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0729 https://doi.org/10.1039/C5AY02298C2015Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplificationApt 4TGGCCAGAGTACGAGTAAGGGAGGTCACAACCTTA35N/AssDNASalmonella Paratyphi AWhole cellDeveloped an aptasensor composed of a designed aptamer (DA) and two short FAM-modified sequences (probe 1 and probe 2) for fluorimetric determination of S. paratyphi A via DNase I-mediated cyclic signal amplification.Obtained a concentration ranging from 1 × 10(2) to 1 × 10(11) cells/mL with a detection limit of 1 × 10(2) cells/mL.N/AN/AN/ABiosensorN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_0730 274242152016Gold nanoparticle-DNA aptamer conjugate-assisted delivery of antimicrobial peptide effectively eliminates intracellular Salmonella enterica serovar TyphimuriumAptHis (6H7)GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC40N/AssDNASalmonella typhimurium infected HeLa cellsS. typhimurium Whole cellHis-aptamer conjugated AuNP platform for the loading of His-tagged AMP (C-terminally hexahistidine-tagged A3-APO (A3-APOHis) AMPs) to eliminate intracellular S. Typhimurium cells.80% viability increase of infected HeLa cells and 100% survival rate of infected mice.N/AN/AN/ATargeted Delivery/TherapeuticsN/A3'-Thiolated (A10-Thiol) and Cyanine5 (Cy5) LabeledAuNP-Apt(His)-A3-APO(His) led to the increased viability of S. Typhimurium-infected HeLa cells.∼80% of A3-APOHis remained in serum after 48 h of incubation with AuNP-Apt(His)-A3-APO(His).N/AN/AN/A
ABdb_0760 https:doi.org10.1016j.carbon.2016.04.0142016Biodegradable graphene oxide and polyaptamer DNA hybrid hydrogels for implantable drug deliveryPolyaptamer (PA)ATCGGCTAGCACGTACAGAACTAAAAAAAAAAAA-GTCGGCTTAGCCTCAACCCCC-AAGGCAAAAAGGCAT70N/AssDNAEscherichia Coli (E. Coli) and Staphylococcus aureus (S. aureus)Kanamycin (Kan)Kan-loaded PA-GO (Kan/PA-GO) hybrid hydrogels for antibacterial effects.Significantly reduced the viability of E. coli and S. aureus to 24.2 ± 4.8% and 17.7 ± 0.7%, respectively.N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-PhosphateN/AN/AN/AN/AN/A
ABdb_0773 274278912016An aptamer cocktail-functionalized photocatalyst with enhanced antibacterial efficiency towards target bacteriaE1GCAATGGTACGGTACTTCCACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli)Whole cellComposite (TiO2-Apc) for enhanced photodynamic inactivation of target-specific bacteria.TiO₂-Apc particles killed approximately 80% of E. coli after 15 min, whereas the raw TiO₂ particles killed only 20% and the TiO₂-Aps particles killed 60% of E. coli after 15 min of UV irradiation.N/AFluorescence Spectroscopy12.4 nMTherapeuticsN/A3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0774 274278912016An aptamer cocktail-functionalized photocatalyst with enhanced antibacterial efficiency towards target bacteriaE2GCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli)Whole cellEnhanced photodynamic inactivation via aptamer-functionalized TiO₂ particles.TiO₂-Apc particles killed approximately 80% of E. coli after 15 min, whereas the raw TiO₂ particles killed only 20% and the TiO₂-Aps particles killed 60% of E. coli after 15 min of UV irradiation.N/AFluorescence Spectroscopy25.2 nMTherapeuticsN/A3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0775 274278912016An aptamer cocktail-functionalized photocatalyst with enhanced antibacterial efficiency towards target bacteriaE10GCAATGGTACGGTACTTCCGTTGCACTGTGCGGCCGAGCTGCCCCCTGGTTTGTGAATACCCTGGGCAAAAGTGCACGCTACTTTGCTAA90N/AssDNAEscherichia Coli (E. Coli)Whole cellEnhanced photodynamic inactivation via aptamer-functionalized TiO₂ particles.TiO₂-Apc particles killed approximately 80% of E. coli after 15 min, whereas the raw TiO₂ particles killed only 20% and the TiO₂-Aps particles killed 60% of E. coli after 15 min of UV irradiation.N/AFluorescence Spectroscopy14.2 nMTherapeuticsN/A3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0784 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8ATACCAGCTTATTCAATT-AGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT-ACAATCGTAATCAGTTAG765'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.PA#2/8 and its truncated variant PA#2/8[S1-58] were able to differentiate between the highly Protein A-producing S. aureus Cowan strain.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)101.4 ± 5.9 nM (of 3′-biotinylated)  and 189.9 ± 13.0 nM (of 5′-biotinylated)DetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0785 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT585'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.PA#2/8 and its truncated variant PA#2/8[S1-58] were able to differentiate between the highly Protein A-producing S. aureus Cowan strain.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)11.3 ± 1.4 nM (of 3′-biotinylated)  and 23.7 ± 2.0 nM (of 5′-biotinylated)DetectionN/A5'-and 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0786 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-50]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTC505'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0787 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S1-43]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGG435'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0788 276505762016G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONAPA#2/8[S28-50]AGGGGGATAGAGGGGGTGGGTTC235'-ATACCAGCTTATTCAATT-N40-ACAATCGTAATCAGTTAG-3'ssDNAStaphylococcus aureus (S. aureus) (P3838, P6031)Staphylococcus aureus Protein A (SpA)Develop ELONA to recognise and bind to Staphylococcus aureus presenting Protein A on the cell surface.N/AN/AEnzyme-Linked Oligonucleotide Assay (ELONA)N/ADetectionN/A5'-and 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0789 265925932016Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7EA1CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDevelop aptamer conjugated graphene oxide (GO)/iron nanocomposites for guanine chemiluminescence detection of E. Coli O157:H7.N/AN/AN/AN/ABiosensorN/A3'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0790 265925932016Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7EA2CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDevelop aptamer conjugated graphene oxide (GO)/iron nanocomposites for guanine chemiluminescence detection of E. Coli O157:H7.N/AN/AN/AN/ABiosensorN/A3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0791 265925932016Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7EA3CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDevelop aptamer conjugated graphene oxide (GO)/iron nanocomposites for guanine chemiluminescence detection of E. Coli O157:H7.N/AN/AN/AN/ABiosensorN/A3'-Pacific BlueN/AN/AN/AN/AN/A
ABdb_0792 265925932016Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7EA4CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDevelop aptamer conjugated graphene oxide (GO)/iron nanocomposites for guanine chemiluminescence detection of E. Coli O157:H7.N/AN/AN/AN/ABiosensorN/A3'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0793 265925932016Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7EA5CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDevelop aptamer conjugated graphene oxide (GO)/iron nanocomposites for guanine chemiluminescence detection of E. Coli O157:H7.N/AN/AN/AN/ABiosensorN/A3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0794 265925932016Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7EA6GGGGGGTTTTCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG52N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDevelop aptamer conjugated graphene oxide (GO)/iron nanocomposites for guanine chemiluminescence detection of E. Coli O157:H7.The limit of detection (LOD) was as low as 4.5×10(3) cfu/ml, with a linear range of 10(4)-10(7) cfu/ml.N/AN/AN/ABiosensorN/A3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0795 263345902016Label-free and highly sensitive electrochemical detection of E. coli based on rolling circle amplifications coupled peroxidase-mimicking DNAzyme amplificationAnti-E. coli aptamerGCAATGGTACGGTACTTCCACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellLabel-free electrochemical biosensor using RCA coupled DNAzyme amplification for detecting E. coli.Exhibits detection limits of 8 cfu/mL and a detection range of 5 orders of magnitude.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0805 273185662016Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugatesAptamerGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAptamer-functionalized silica magnetic nanoparticles-based assay for on-site determination of S. aureus.Detects S. aureus cells as low as 682 CFU in whole blood, with a linear range from 800 CFU/ml blood to 104 CFU/ml blood.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0806 265998602016Salmonella typhimurium detection using a surface-enhanced Raman scattering-based aptasensorApt1AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA SERS-based aptasensor bearing Au@Ag core/shell nanoparticles (NPs) functionalized with apt 1 and X-rhodamine (ROX)-modified apt 2 was developed for the detection of bacteria.A calibration curve is obtained in the range of 15 to 1.5 × 10(6) CFU/mL with a limit of detection of 15 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0807 265998602016Salmonella typhimurium detection using a surface-enhanced Raman scattering-based aptasensorApt2AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA SERS-based aptasensor bearing Au@Ag core/shell nanoparticles (NPs) functionalized with apt 1 and X-rhodamine (ROX)-modified apt 2 was developed for the detection of bacteria.A calibration curve is obtained in the range of 15 to 1.5 × 10(6) CFU/mL with a limit of detection of 15 CFU/mL.N/AN/AN/ABiosensorN/A5'-X-rhodamine (ROX) modifiedN/AN/AN/AN/AN/A
ABdb_0808 268949872016Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sampleAptamer (N45)TTTGGTCCTTGTCTTATGTCCAGAATGCGAGGAAAGTCTATAGCAGAGGAGATGTGTGAACCGAGTAAATTTCTCCTACTGGGATAGGTGGATTAT96N/AssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (OMP)Developed a label-free impedimetric aptamer-based biosensor for S. typhimurium detection.Rapid (30 min) detection with a concentration range of 1×10(1) to 1×10(8) CFU/mL and limit of detection (LOD) of 6 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0809 274117752016Rapid and Selective Detection of Pathogenic Bacteria in Bloodstream Infections with Aptamer-Based RecognitionApt-S. aureusTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAACTTTTTTTTT71N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAptamer-based capture platform using Fe3O4@mTiO2 magnetic nanoparticles for the detection of bacteria.Display a favourable bacterial-capture efficiency of about 80% even at low infectious doses (10–2000 CFU/mL).N/AN/AN/ADetectionN/A3'-Amidation (NH₂-(CH2)7) and 3'-FAM Labeled ((CH2)7-FAM)N/AN/AN/AN/AN/A
ABdb_0810 274117752016Rapid and Selective Detection of Pathogenic Bacteria in Bloodstream Infections with Aptamer-Based RecognitionApt-E. coliATCCGTCACACCTGCTCTACTGGCCGGCTCAGCATGACTAAGAAGGAAGTTATGTGGTGTTGGCTCCCGTATTTTTTTTTT81N/AssDNAEscherichia Coli (E. Coli)Whole cellAptamer-based capture platform using Fe3O4@mTiO2 magnetic nanoparticles for the detection of bacteria.Display a favourable bacterial-capture efficiency of about 80% even at low infectious doses (10–2000 CFU/mL).N/AN/AN/ADetectionN/A3'-Amidation (NH₂-(CH2)7)N/AN/AN/AN/AN/A
ABdb_0811 274047352016Highly sensitive detection of lipopolysaccharides using an aptasensor based on hybridization chain reactionDetection probeGTCGAATGCTCTGCCTGGAAGAGTTGTTAGCAGGGA36N/AssDNAEscherichia Coli (E. Coli) O111:B4Lipopolysaccharide (LPS)Developed an aptasensor assay based on hybridization chain reaction (HCR) to detect LPS.Display a relatively low detection limit (1.73 ng/mL) with a linear response range of 1–10(5) ng/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0812 266411082016Dual Recognition Strategy for Specific and Sensitive Detection of Bacteria Using Aptamer-Coated Magnetic Beads and Antibiotic-Capped Gold NanoclustersSA-aptamerGCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellAptamer-coated magnetic beads (Apt-MB) and Van-functionalized fluorescent gold nanoclusters (AuNCs@Van) were employed for specific capture of SA.Sensitive quantification of SA in the range of 32–10(8) cfu/mL with the detection limit of 16 cfu/mL via fluorescence intensity.N/AN/AN/ABiosensorN/A5′-Biotinylated and 5′-Cyanine3 (Cy3) and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0813 274275912016Duplex Identification of Staphylococcus aureus by Aptamer and Gold NanoparticlesSA23GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA45N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDevelop an aptamer-AuNPs-based colorimetric method for duplex identification of S. aureus.AuNPs as a sensor could well distinguish S. aureus from S. enteritidis and P. mirabilis.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0816 270637162016Magnetic Nanoparticles-based Aptasensor Using Gold Nanoparticles as Colorimetric Probes for the Detection of Salmonella typhimuriumS. typhimurium aptamerATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor for S. typhimurium detection using gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs).The assay shows a linear response over a range of 25 to 10(5) cfu/mL, and the detection limit was improved to 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (for Apt1) and 5'-Biotinylated (for Apt2)N/AN/AN/AN/AN/A
ABdb_0817 269712742016Dual-excitation upconverting nanoparticle and quantum dot aptasensor for multiplexed food pathogen detectionS. aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDual-excitation aptasensing platform based on the luminescent nanoparticles (QDs and UCNPs) for the detection of Salmonella typhimurium and Staphylococcus aureus.Linear detection range: 50 to 10⁶ cfu/mL; LOD: 16 cfu/mL (S. aureus).N/AN/A35 nMBiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0818 269712742016Dual-excitation upconverting nanoparticle and quantum dot aptasensor for multiplexed food pathogen detectionS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDual-excitation aptasensing platform based on the luminescent nanoparticles (QDs and UCNPs) for the detection of Salmonella typhimurium and Staphylococcus aureus.Linear detection range: 50 to 10⁶ cfu/mL; LOD: 28 cfu/mL (S. typhimurium).N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0819 272095742016A paper based graphene-nanocauliflower hybrid composite for point of care biosensingRNA AptamerGGGUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAUAGAUAGUAA64N/AssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)O-antigenDevelop electrochemical biosensing platform using graphene paper functionalized with fractal platinum nanocauliflower for detection of E. coli O157:H7.Detection limit (LOD) of ~4 CFU/mL with linear range of 4 to 10(5) CFU/mL and a response time of 12 min.N/AN/A110 nMBiosensorN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0820 https://doi.org/10.1021/acssensors.6b003332016Quantitative Label-Free Listeria Analysis Based On Aptamer Modified Nanoporous SensorLM AptamerATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria MonocytogenesWhole cellDeveloped a nanoporous sensor with aptamer combined porous anodic aluminium oxide membrane for detection of L. monocytogenes.Achieved detection within 10 min, with a high sensitivity of 100 CFU/mL and a good linear range between 100 and 1250 CFU/mL.N/AN/AN/ABiosensorN/A5′-CHON/AN/AN/AN/AN/A
ABdb_0821 https://doi.org/10.1016/j.foodcont.2015.11.0312016Vibrio parahaemolyticus detection aptasensor using surface-enhanced Raman scatteringAptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellA SERS-based aptasensor was developed using Au@Ag core-shell nanoparticles for the detection of V. parahaemolyticus.Achieved a concentration in the linear range of 10–10(6) cfu/mL, with a limit of detection of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (for apt 1) and 5'-Cyanine3 (Cy3) Labeled (for apt 2)N/AN/AN/AN/AN/A
ABdb_0822 https://doi.org/10.1039/C6AY02623K2016SERS aptasensor detection of Salmonella typhimurium using a magnetic gold nanoparticle and gold nanoparticle based sandwich structureS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 50761)Whole cellDeveloped a SERS aptasensor using aptamer-functionalized magnetic gold nanoparticles (MGNPs) and mercaptobenzoic acid (4-MBA) functionalized gold nanoparticles (GNPs) based sandwich structure for the detection of S. typhimurium.Exhibit a good linear range from 10 cfu/mL to 10(7) cfu/mL, and the LOD is 5 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0832 282066802017A single-stranded DNA aptamer against mannose-capped lipoarabinomannan enhances anti-tuberculosis activity of macrophages through downregulation of lipid-sensing nuclear receptor peroxisome proliferator-activated receptor γ expressionZXL1GCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GGCGCCATAGCGACGGGGCCATTCCAAGAA-GGGTCAATGCGTCATA88N/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Mannose-capped lipoarabinomannan (ManLAM) epitopeDownregulate PPARγ expression by blocking the binding of ManLAM to MR on macrophages, and ZXL1 enhances IL-1β and IL-12 mRNA expression and cytokine production in ManLAM-treated macrophages but decreases IL-10 production.The percentage of macrophages that took up iH37Rv decreased from 15.9% to 7.2%, thereby inhibiting ManLAM-induced immunosuppression of DCs.N/AN/AN/ATherapeutics/ImmunmodulatoryN/ABiotinylatedN/AN/AN/AN/AN/A
ABdb_0833 287780622017DNA aptamer functionalized gold nanostructures for molecular recognition and photothermal inactivation of methicillin-Resistant Staphylococcus aureusAptamerATCCAGACGTGACGCAGC-ATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTG-TGGACACGGTGGCTTAGTA755'-ATCCAGACGTGACGCAGC-N38-TGGACACGGTGGCTTAGTA-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA) standard strain 43300Whole cellAptamer-functionalized gold nanorods (Apt@Au NRs) for inactivation of MRSA with targeted photothermal therapy (PTT).Over 95% inactivation of MRSA cells within 2 min.N/AN/AN/ATherapeuticsN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0918 281195142017High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis AgentsAntibac1TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC55N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922), Klebsiella Pneumoniae (ATCC 27853), Proteus Mirabilis (ATCC 00557), Pseudomonas Aeruginosa (ATCC 700603), Staphylococcus aureus (S. aureus) (ATCC 29213), Streptococcus Pneumoniae (ATCC 23855), and Enterococcus Faecium (ATCC 29212), and Proteus Vulgaris, Morganella Morganii, Citrobacter Freundii, and Acinetobacter BaumanniiPeptidoglycanDevelop generic probes as biological recognition elements in biosensors for sepsis diagnosis in the clinical setting.Antibac1 bound with higher efficiency to both gram-positive (S. aureus, S. penumoniae, L. monocytogenes) and gram-negative (A. baumannii, C. freudii, K. pneumoniae, P. aeruginosa, P. mirabilis).N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)268.50 ± 54.34 nM (for A. baumannii); 51.74 ± 11.75 nM (for L. monocytogenes); 31.82 ± 4.38 nM (for E.coli); 170.10 ± 32.13 nM (for S.aureus) and 256.10 ± 47.89 nM (for K. pneumoniae)DiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_0919 281195142017High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis AgentsAntibac2TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC55N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922), Klebsiella Pneumoniae (ATCC 27853), Proteus Mirabilis (ATCC 00557), Pseudomonas Aeruginosa (ATCC 700603), Staphylococcus aureus (S. aureus) (ATCC 29213), Streptococcus Pneumoniae (ATCC 23855), and Enterococcus Faecium (ATCC 29212), and Proteus Vulgaris, Morganella Morganii, Citrobacter Freundii, and Acinetobacter BaumanniiPeptidoglycanDevelop generic probes as biological recognition elements in biosensors for sepsis diagnosis in the clinical setting.Antibac2 bound with higher efficiency to the gram-positive species S. aureus and E. faecium and to the gram-negative species (A. baumannii, C. freudii, K. pneumoniae, M. moraganii, P. aeruginosa, P. mirabilis, and E. coli).N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)71.92 ± 9.74 nM (for A. baumannii); 54.19 ± 12.09 nM (for L. monocytogenes); 62.43 ± 11.97 nM (for E.coli); 194.90 ± 38.55 nM (for S.aureus) and 195.90 ± 42.91 nM (for K. pneumoniae)DiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_0932 276965542017Evaluation of Staphylococcus aureus DNA aptamer by enzyme-linked aptamer assay and isothermal titration calorimetrySA31TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA45N/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellELAA and ITC to study impact of temperature and ion concentration in the binding of aptamer to its target on surface of bacteria.Both temperature and salt concentration directly influenced the 3D structure of the DNA molecule under specified experimental conditions, thereby altering the aptamer's affinity for its target.N/AN/AN/ADetectionN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0934 278258862017Upconversion nanoparticles based FRET aptasensor for rapid and ultrasenstive bacteria detectionE.coli aptamerGCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (ATCC 8739)Whole cellUpconversion nanoparticles (UCNPs) based FRET aptasensor (UCNPs-cDNA from AuNPs-aptamers) for detecting E. coli in tap/pond water and milk.Display a detection range of 5–10(6) cfu/mL and a detection limit of 3 cfu/mL in 20 min.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0948 284149752017Fullerene-doped polyaniline as new redox nanoprobe and catalyst in electrochemical aptasensor for ultrasensitive detection of Mycobacterium tuberculosis MPT64 antigen in human serumMBA ITGGGAGCTGATGTCGCATGGGTTTTGATCACATGA35N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinElectrochemical aptasensor based on GNPs-C60-PAn labelled with signal aptamer for the determination of MTB antigen.A wide linear detection range of 0.02 to 1000 pg/mL was obtained, with a detection limit of 20 fg/mL for MPT64.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0949 284149752017Fullerene-doped polyaniline as new redox nanoprobe and catalyst in electrochemical aptasensor for ultrasensitive detection of Mycobacterium tuberculosis MPT64 antigen in human serumMBA IITTCGGGAATGATTATCAAATTTATGCCCTCTGAT34N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinElectrochemical aptasensor based on GNPs-C60-PAn labelled with signal aptamer for the determination of MTB antigen.A wide linear detection range of 0.02 to 1000 pg/mL was obtained, with a detection limit of 20 fg/mL for MPT64.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0950 https://doi.org/10.1007/s00604-017-2142-22017A magnetic relaxation switch aptasensor for the rapid detection of Pseudomonas aeruginosa using superparamagnetic nanoparticlesAptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellDevelop a magnetic relaxation switch (MRSw) aptasensor (SPIO-aptamer) for the determination of Pseudomonas aeruginosa in real food and drinking water samples.Exhibit a linear range from 10(0) cfu/mL to 10(6) cfu/mL, and a detection limit of 50 cfu/mL was obtained within 40 mins.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0951 https://doi.org/10.1016/j.snb.2017.09.1212017Novel impedimetric aptasensor for label-free detection of Escherichia coli O157:H7E. coli specific aptamerATCCGTCACACCTGCTCTGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCGTGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped a novel impedance-based aptasensor for the detection of E. coli O157:H7.Exhibited a broad range from 10(1) to 10(5) cfu/mL with the LOD about 2.9 × 10(2) cfu/mL with a detection time of 30 min.N/AN/AN/ABiosensorN/A5'-disulfide-modifiedN/AN/AN/AN/AN/A
ABdb_0952 https://doi.org/10.1016/j.foodcont.2017.07.0162017SERS aptasensor for Salmonella typhimurium detection based on spiny gold nanoparticlesS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a novel SGNPs-based SERS aptasensor functionalized with p-MBA for the detection of S. typhimurium.Display linear range 10(1) cfu/mL to 10(5) cfu/mL, with a limit of detection (LOD) of 4 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated or BiotinylatedN/AN/AN/AN/AN/A
ABdb_0953 291608512017Development of An Impedimetric Aptasensor for the Detection of Staphylococcus aureusPA#2/8[S1-58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) (DSM 20231)Staphylococcus aureus Protein A (SpA)Developed an impedimetric aptasensor for the detection of S. aureus.Showed a limit of detection of 10 CFU/mL within 10 minutes.N/AElectrochemical impedance spectroscopy (EIS)18.5 ± 1.8BiosensorN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0954 290516202017Antimicrobial peptide-loaded gold nanoparticle-DNA aptamer conjugates as highly effective antibacterial therapeutics against Vibrio vulnificusApt(His)GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC40N/AssDNAVibrio Vulnificus (MO6-24/O)V. vulnificus-infected HeLa cellsThe AuNP-AptHis conjugate delivers the HPA3P(His) peptide into host cells, inducing bacterial cell death by disrupting membrane integrity.Compared with that of buffer- or AuNP-AptHis-incubated cells, the number of viable V. vulnificus cells in the AuNP-AptHis-HPA3PHis-treated cells decreased by 90% in 10 min. All infected mice injected with AuNP-AptHis-HPA3PHis survived until 120 h.N/AN/AN/ATargeted Delivery/TherapeuticsN/A3'-Thiolated (A10-Thiol)HPA3PHis and AuNP-Apt(His)-HPA3P(His) did not exhibit cytotoxicity against HeLa cells and mouse erythrocytes up to 10 µM.N/AN/AN/AN/A
ABdb_0955 290712022017An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7E. coli aptamerCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellDevelop an electrochemical aptasensor for rapid and sensitive detection of E. coli O157:H7 that relies on aptamer and urease-modified GNPs.Able to detect E. coli as low as 10(1) CFU/mL in 3 h, and the mean recovery of E. coli in the spiked pasteurized milk was ~99%.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0956 288034752017Intuitive Label-Free SERS Detection of Bacteria Using Aptamer-Based in Situ Silver Nanoparticles SynthesisS.aureus-AptamerSTCCCTACGGCGCTAACCTCCCAACCGCTCCACCCTGCCTCCGCCTCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (CICC 21600)Whole cellDeveloped a SERS-based aptasensor using aptamer@AgNPs for the detection of bacteria.Exhibited a linear concentration, ranging from 10(1) to 10(7) cfu/mL with the detection limit of 1.5 cfu/mL.N/AN/AN/ABiosensorN/AFAM LabeledN/AN/AN/AN/AN/A
ABdb_0957 288034752017Intuitive Label-Free SERS Detection of Bacteria Using Aptamer-Based in Situ Silver Nanoparticles SynthesisL.mono-AptamerLTACTATCGCGGAGACAGCGCGGGAGGCACCGGGGA35N/AssDNAListeria Monocytogenes (CICC 21633)Whole cellDeveloped a SERS-based aptasensor using aptamer@AgNPs for the detection of bacteria.N/AN/AN/AN/ABiosensorN/AFAM LabeledN/AN/AN/AN/AN/A
ABdb_0958 281076862017A novel aptasensor for the colorimetric detection of S. typhimurium based on gold nanoparticlesS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellColorimetric aptasensor was developed based on color changing GNPs for the detection of S. typhimurium.Quantitative detection of S. typhimurium from 10(2) to 10(7) cfu/mL with a detection limit as low as 56 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0959 281079302017A chemiluminescent aptasensor based on rolling circle amplification and Co2+/N-(aminobutyl)-N-(ethylisoluminol) functional flowerlike gold nanoparticles for Salmonella typhimurium detectionS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellChemiluminescent aptasensor based on rolling circle amplification (RCA) using Co2+/ABEI-AuNFs-cDNA complex was developed for the detection of S. typhimurium.The limit of detection (LOD) was 10 cfu/mL. The method exhibited excellent selectivity over non-target bacteria.N/AN/AN/ABiosensorN/A3'-Biotinylated (Biotin-TTTTTT)N/AN/AN/AN/AN/A
ABdb_0960 290306712017Graphene-based label-free electrochemical aptasensor for rapid and sensitive detection of foodborne pathogenS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellrGO-azophloxine (AP) nanocomposite aptasensor was developed for the detection of foodborne pathogens.Exhibited a linear range of detection from 10(8) to 10(1) cfu/mL with a detection limit of 10(1) cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AAptasensor showed good stability for up to 20 days, with a 5.1% increase in current response when stored in ultrapure water at 4°C.N/AN/AN/A
ABdb_0961 286457562017An aptamer-based PCR method coupled with magnetic immunoseparation for sensitive detection of Salmonella Typhimurium in ground turkeyS. Typhimurium aptamerCAGTCCAGGACAGATTCGCGAGCCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATCCACGTGGATTTCATTCAGCGATT90N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-based PCR method coupled with magnetic immunoseparation was developed to detect S. Typhimurium from ground turkey.Able to detect 10(2) CFU/mL of S. Typhimurium in pure culture, and 10(3) CFU/mL of S. Typhimurium in ground turkey.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0962 295947122017Fluorometric graphene oxide-based detection of Salmonella enteritis using a truncated DNA aptamerSE54FTACCAAAATGTTGGATTGGATGTTGTACTGGGTTGCATAGGTAGTCCAGAAGCC54N/AssDNASalmonella Enteritidis (S. Enteritidis) (ATCC 13076)Whole cellTruncated aptamer was used to develop a fluorometric graphene oxide (GO) based assay for the detection of S. enteriditis.LOD of 38 and 25 cfu/mL for the full length SE54 and SE54T, respectively, with a linear response from 10(2) to 10(7) cfu/mL.N/AFluorescence Spectroscopy6.3 nMDetectionN/A5'-Fluorescein LabeledN/AN/AN/AN/AN/A
ABdb_0963 295947122017Fluorometric graphene oxide-based detection of Salmonella enteritis using a truncated DNA aptamerSE54TGGATTGGATGTTGTACTGGGTTGCATAGG29N/AssDNASalmonella Enteritidis (S. Enteritidis) (ATCC 13076)Whole cellTruncated aptamer was used to develop a fluorometric graphene oxide (GO) based assay for the detection of S. enteriditis.LOD of 38 and 25 cfu/mL for the full length SE54 and SE54T, respectively with a linear response from 10(2) to 10(7) cfu/mL.N/AFluorescence Spectroscopy3.2 nMDetectionN/A5'-Fluorescein LabeledN/AN/ABest CandidateN/AN/A
ABdb_0964 289878792017Thioaptamer targeted discoidal microparticles increase self immunity and reduce Mycobacterium tuberculosis burden in miceCD44 Thioaptamer (CD44TA)GAGATTCATCACGCGCATAGTCTTGGGA*CGGTGTTA*A*A*CGA*A*A*GGGGA*CGA*CCCGA*CTA*TGCGA*TGA*TGTCTTC74N/AssDNAMycobacterium Tuberculosis (M.Tb.)CD44 receptor (Mtb-infected murine macrophages)Develop a targeting system using CD44 thioaptamers (TA)-conjugated discoidal silicon mesoporous microparticles (SMP) to enhance accumulation of these agents/carriers in the infected macrophages in the lungs.CD44TA-SMP treatment increased IP-10 (CXCL-10), MCP-1, MCP-2, and RANTES, with no change in IL-6 release compared to the control.N/AN/AN/ATherapeuticsN/A*= 5'-monothio-dA and 5'-Cyanine5 (Cy5) LabeledIn uninfected murine and human macrophages, SMP and CD44TA-SMP did not affect the cell viability.N/AN/AN/AN/A
ABdb_0965 282877152017Dual-Recognition Förster Resonance Energy Transfer Based Platform for One-Step Sensitive Detection of Pathogenic Bacteria Using Fluorescent Vancomycin-Gold Nanoclusters and Aptamer-Gold NanoparticlesAptamerGCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Bacterial cell wallDevelop a dual-molecular FRET platform based on the recognition of bacterial cell walls by antibiotic and aptamer molecules, respectively, for the detection of pathogenic bacteria.Shows a linear concentration of Staph. aureus in the range from 20 to 10(8) cfu/mL with a detection limit of 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated and Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_0966 https://doi.org/10.1016/j.snb.2017.07.0742017A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphoneDirect Dot-Blot AptamerGGCGCCATAGCGACGGGGCCATTCCAAGAA30N/AssDNAMycobacterium Tuberculosis (H37Ra)Mannose-capped lipoarabinomannan (ManLAM) epitopeSmartphone-based method using enzyme-linked aptamers for point-of-need M.tb detection.The direct dot-blot system provided a lower limit of quantitation of 10(4) CFU/mL and greater accuracy.N/AN/AN/ADetectionN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0967 https://doi.org/10.1016/j.snb.2017.07.0742017A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphoneIndirect Dot-Blot AptamerGCGGAATTCTAATACGACTCACTATAGGGAACAGTCCGAGCC-GGCGCCATAGCGACGGGGCCATTCCAAGAA-GGGTCAATGCGTCATA88N/AssDNAMycobacterium Tuberculosis (H37Ra)Mannose-capped lipoarabinomannan (ManLAM) epitopeSmartphone-based method using enzyme-linked aptamers for point-of-need M.tb detection.The limit of quantitation (LOQ) for indirect assays was 10(5) CFU/mL.N/AN/AN/ADetectionN/AN/AN/AN/AN/AN/AN/A
ABdb_0968 278165852017A rapid and visual aptasensor for Lipopolysaccharides detection based on the bulb-like triplex turn-on switch coupled with HCR-HRP nanostructuresBLA1TCTCCCTTTAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCTTTTCCCTCT57N/AssDNAEscherichia Coli (E. Coli) O111:B4 and O111:B5Lipopolysaccharide (LPS)Developed a turn-on sensor based on a bulb-like triplex turn-on switch (BTTS) for the detection of lipopolysaccharides.N/AN/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0969 278165852017A rapid and visual aptasensor for Lipopolysaccharides detection based on the bulb-like triplex turn-on switch coupled with HCR-HRP nanostructuresBLA 2TCTCTCCCTTTAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCTTTTCCCTCTCT61N/AssDNAEscherichia Coli (E. Coli) O111:B4 and O111:B5Lipopolysaccharide (LPS)Developed a turn-on sensor based on a bulb-like triplex turn-on switch (BTTS) for the detection of lipopolysaccharides.N/AN/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0970 278165852017A rapid and visual aptasensor for Lipopolysaccharides detection based on the bulb-like triplex turn-on switch coupled with HCR-HRP nanostructuresBLA 3TCTCTCTCCCTTTAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCTTTTCCCTCTCTCT65N/AssDNAEscherichia Coli (E. Coli) O111:B4 and O111:B5Lipopolysaccharide (LPS)Developed a turn-on sensor based on a bulb-like triplex turn-on switch (BTTS) for the detection of lipopolysaccharides.The sensor has a linear calibration range of 1-150 ng/mL and a detection limit of 50 pg/mL, quantitatively, with the portable spectrophotometer, and 20 ng/mL, semi-quantitatively, with the naked eye.N/AN/AN/ABiosensorN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_0971 278165852017A rapid and visual aptasensor for Lipopolysaccharides detection based on the bulb-like triplex turn-on switch coupled with HCR-HRP nanostructuresBLA 4TCTCTCTCTCCCTTTAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCTTTTCCCTCTCTCTCT69N/AssDNAEscherichia Coli (E. Coli) O111:B4 and O111:B5Lipopolysaccharide (LPS)Developed a turn-on sensor based on a bulb-like triplex turn-on switch (BTTS) for the detection of lipopolysaccharides.N/AN/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0972 289106782017Colorimetric aptasensor for the detection of Salmonella enterica serovar typhimurium using ZnFe2O4-reduced graphene oxide nanostructures as an effective peroxidase mimeticsApt 1 (Capture)AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellThe colorimetric aptasensor platform was developed based on ZnFe2O4/rGO nanostructures, which act as an artificial enzyme (mimetic) that catalyses the oxidation of TMB by H2O2 to detect S. typhimurium.The limit of detection (LOD) of 11 cfu/mL, and the detection range was 11 to 1.10 × 10(5) cfu/mL of S. typhimurium in buffer solution.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0973 289106782017Colorimetric aptasensor for the detection of Salmonella enterica serovar typhimurium using ZnFe2O4-reduced graphene oxide nanostructures as an effective peroxidase mimeticsApt 2 (Signal)AAAAAAAAAAAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA52N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellThe colorimetric aptasensor platform was developed based on ZnFe2O4/rGO nanostructures, which act as an artificial enzyme (mimetic) that catalyses the oxidation of TMB by H2O2 to detect S. typhimurium.Limit of detection (LOD) of 11 cfu/mLN/AN/AN/ABiosensorN/A5'-PolyA (12A)N/AN/AN/AN/AN/A
ABdb_0974 279514522017(1→3)-β-D-glucan aptamers labeled with technetium-99m: Biodistribution and imaging in experimental models of bacterial and fungal infectionSeq6CGACTGACGCCTCCAAGCCACACGTGTGAGAAGGGTGTTATCATGTATTTCGTGTTCCTTTCGTCATTCCTTTGTCTGCATGCATCGCTACGTG94N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)(1 → 3)-β-D-glucansRadiolabeled aptamers were evaluated to identify infectious foci caused by bacterial cells.The target/non-target ratios were 3.17 ± 0.22 for seq6 in S. aureus-infected animals.N/AN/A0.303 ± 0.067 μMImagingN/A5'-Radiolabelled (99mTc labeled)N/AIn vitro stability of seq6 aptamer labelled with 99mTc in the presence of 0.9% saline, plasma, and cysteine excess (molar ratio) was above 84.2 ± 2.9% in all cases at 24hrs.N/AN/AN/A
ABdb_0975 287158742017Detection of bacterial infection by a technetium-99m-labeled peptidoglycan aptamerAntibac1TCGCGCGAGTCGTCTGGGGACAGGGAGTGCGCTGCTCCCCCCGCACGTCCTCCC54N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)PeptidoglycanRadiolabeled aptamers were evaluated for biodistribution studies and scintigraphic imaging in infection‐bearing mice.Achieved a high target-to-non-target (T/NT) ratio of 4.7 ± 0.9 at 1.5 h and 4.6 ± 0.1 at 3.0 h in bacterial-infected models.N/ABinding Assay0.170 ± 0.032 μMImagingN/A5'-Inverted Thymidine (5'-idT), 3'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (99mTc labeled)N/ARadiolabeling yields were superior to 90% and 99mTc‐Antibac1 was highly stable in the presence of saline, plasma, and cysteine up to 6 h.N/ADistribution half-life: 6.7 min and Elimination half-life: 41.3 min.N/A
ABdb_0976 288604622017Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meatAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 2421)Whole cellDevelop a portable LSPR biosensor-based device on gold nanoparticles (AuNPs) for the detection of Salmonella typhimurium in pork meat samples.Limit of detection (LOD) of 10(4) cfu/mL in both pure culture and spiked pork samples, and total analysis time of 30-35 minutes.N/AN/AN/ABiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_0977 286246202017AgBr nanoparticles/3D nitrogen-doped graphene hydrogel for fabricating all-solid-state luminol-electrochemiluminescence Escherichia coli aptasensorsE. coli aptamerGCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped a ECL aptasensor based on amine-functionalized E. coli aptamer and luminol/AgBr/3DNGH for the detection of E. coli.Linear response range from 0.5 to 500 cfu/mL and Limit of Detection (LOD) of 0.17 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/ANo obvious change was observed for the response of a BSA/aptamer/GA/CHIT/luminol/AgBr/3DNGH/GCE when stored at 4°C for 12 d, indicating an acceptable stability of the aptasensor.N/AN/AN/A
ABdb_0978 275263402017Aptamer biosensor for Salmonella typhimurium detection based on luminescence energy transfer from Mn2+-doped NaYF4:Yb, Tm upconverting nanoparticles to gold nanorodsS. typhimurium aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellA LET method based on UCNPs and gold nanorods (Au NRs) was developed for the determination of Salmonella typhimurium.Linear range: 12 to 5×10^5 cfu/mL and Limit of Detection (LOD): 11 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0980 286917952017Copper-Based Metal-Organic Framework Nanoparticles with Peroxidase-Like Activity for Sensitive Colorimetric Detection of Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA colorimetric method for the detection of S. aureus was developed using aptamer-modified Cu-MOF nanoparticles.The linear range for S. aureus detection is 50-10,000 CFU/mL, with a detection limit of 20 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0981 281591082017An enhanced chemiluminescence resonance energy transfer aptasensor based on rolling circle amplification and WS2 nanosheet for Staphylococcus aureus detectionAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellA chemiluminescence resonance energy transfer aptasensor was developed for the detection of S. aureus with Co2+ enhanced N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functional flowerlike gold nanoparticles (Co2+/ABEI-AuNFs) and WS2 nanosheet.The CL signal was found to be linear within the range of 50 cfu/mL to 1.5 × 10(5) cfu/mL, and the limit of detection was 15 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_0997 https://doi.org/10.1007/s12161-017-0864-82017Fabricating a Novel Raman Spectroscopy-Based Aptasensor for Rapidly Sensing Salmonella typhimuriumS. typhimurium aptamer (STA)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a surface-enhanced Raman scattering (SERS) based aptasensor with Raman active molecule attached GNRs and cDNA for the detection of Salmonella typhimurium (ST).Observed linear ST concentration from 56 to 56 × 10(7) cfu/mL with a LOD of 9 cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_0998 287955342017Graphene Field-Effect Transistors for the Sensitive and Selective Detection of Escherichia coli Using Pyrene-Tagged DNA AptamerAptamerGCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (ATCC 8739)Whole cellDeveloped a biosensing platform using graphene field-effect transistors (APG-FETs) with the aid of pyrene-tagged DNA aptamers for E. coli detection.Achieve a detection limit of 10(2) CFU/mL for E. coli within 72 s.N/AN/AN/ABiosensorN/A5'-(pyrene derivate)-TTTTN/AN/AN/AN/AN/A
ABdb_0999 https://doi.org/10.1007/s00604-017-2383-02017Rolling circle amplification based amperometric aptamer/immuno hybrid biosensor for ultrasensitive detection of Vibrio parahaemolyticusVp-aptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped an antibody-aptamer-based hetero-sandwich amperometric biosensor for the detection of V. parahaemolyticus.Achieved a range of 2.2 to 2.2 x10(8) cfu/mL, and the limit of detection is as low as 2 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AAfter 2 weeks of storage at 4°C, the fabricated electrochemical aptasensor retains about 95.2% of its initial sensing current response.N/AN/AN/A
ABdb_1000 https://doi.org/10.1016/j.jelechem.2017.10.0542017Development of an aptasensor using reduced graphene oxide chitosan complex to detect SalmonellaAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a rGO-CHI electrochemical aptasensor to detect Salmonella.Had a wide detection range of 10(1) to 10(6) CFU/mL with a low limit of detection of 10(1) CFU/mL.N/AN/AN/ABiosensorN/A3'-Thiolated ((C12)-SH) and 5'-Amidation ((C12)-NH₂)N/AN/AN/AN/AN/A
ABdb_1001 https://doi.org/10.1016/j.snb.2017.05.1462017A simple dendrimer-aptamer based microfluidic platform for E. coli O157:H7 detection and signal intensification by rolling circle amplificationAptamerATCCGTCACACCTGCTCTATCAAATGTGCAGATATCAAGACGATTTGTACAAGATGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDeveloped a dendrimer-aptamer microfluidic detection system with Rolling circle amplification (RCA) for the detection of E. coli O157:H7 cells.RCA was able to enhance detection signals by up to 50 times, and the limit of detection of the system was reduced to 10(2) cells/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) and 5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1002 293574052018Combat biofilm by bacteriostatic aptamer-functionalized graphene oxideST-3 (or ST-33)CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG60N/AssDNASalmonella Typhimurium (S. Typhimurium)BiofilmAptamer-GO conjugate reduces the cellular membrane potential to inhibit biofilm formation.93.3 ± 3.4% inhibition ratio at the initial stage of biofilm formation and 84.6 ± 5.1% degradation ratio on formed biofilms.12N/AN/ATherapeuticsN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1066 298966412018Aptamer based SERS detection of Salmonella typhimurium using DNA-assembled gold nanodimersAptamer (ssDNA1)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDevelop SERS-based aptasensor using DNA-assembled gold nanodimers (AuNDs) for detection of S. typhimurium.Display a linear range in the 10(2) to 10(7) cfu/mL, and the limit of detection is 35 cfu/mL within 1hr.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1070 302437662018Aptamer-based fluorometric assay for direct identification of methicillin-resistant Staphylococcus aureus from clinical samplesM23CCATCCACACTCCGCAAGGGTGCCCCGGGGGGCTGTTCAGCGTGGTGGTGGGATGCCGTTTTGGTCCTTAGTCTCCGTCGTCGGCTGCCTCTACAT96N/AssDNAMethicillin-resistant Staphylococcus aureus strain N315 (MRSA)Penicillin binding protein 2a (PBP2a)Aptamer-based fluorometric assay for detection of MRSA in clinical samples coupled with immunomagnetic separation.Exhibited a detection limit of 2.63 × 10(3) and 1.38 × 10(3) CFU/mL in PBS and spiked nasal swab, respectively, within 2 h.N/AN/A21.9  nMBiosensorN/A5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1072 300141632018Selective capture and sensitive fluorometric determination of Pseudomonas aeruginosa by using aptamer modified magnetic nanoparticlesP. aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellA fluorometric assay for the detection of the food pathogen P. aeruginosa based on hybridization of aptamer and FAM-cDNA (aptamer&FAM-cDNA@MNPs).Display a linear range between 10 and 10(8) cfu/mL, with a detection limit as low as 1 cfu/mL. The detection process can be finished within <1.5 h.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1074 302479072018Graphene Oxide Quantum Dots Assisted Construction of Fluorescent Aptasensor for Rapid Detection of Pseudomonas aeruginosa in Food SamplesP.aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 15442)Whole cellDevelop a fluorescent aptasensor based on DNA hybridization and fluorescence resonance energy transfer for the detection of P. aeruginosa.Shows a wide linear range of 1.28 × 10(3)-2.00 × 10(7) cfu/mL with a detection limit of 100 cfu/mL within 2 h.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1080 https://doi.org/10.1002/slct.2018010082018Exploiting Stokes and anti-Stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applicationsS. aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTCCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellAptamer-functionalized QD and UCNP nanoprobes conjugated with partially complementary DNA-modified magnetic beads for separation of different bacteria.The limit of detection was 15 cfu/mL for S. aureus with a linear range from 10(2)-10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1081 https://doi.org/10.1002/slct.2018010082018Exploiting Stokes and anti-Stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applicationsL. monocytogenes aptamerTACTATCGCGGAGACAGCGCGGGAGGCACCGGGGA35N/AssDNAListeria Monocytogenes (ATCC 19115)Whole cellaptamer-functionalized QD and UCNP nanoprobes that were conjugated with partially complementary DNA-modified magnetic beads for separation.The limit of detection was 28 cfu/mL for L. monocytogenes with a linear range from 10(2)-10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1082 https://doi.org/10.1002/slct.2018010082018Exploiting Stokes and anti-Stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applicationsP. aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellaptamer-functionalized QD and UCNP nanoprobes that were conjugated with partially complementary DNA-modified magnetic beads for separation.The limit of detection was 25 cfu/mL for P. aeruginosa with a linear range from 10(2)-10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1083 https://doi.org/10.1002/slct.2018010082018Exploiting Stokes and anti-Stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applicationsS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellaptamer-functionalized QD and UCNP nanoprobes that were conjugated with partially complementary DNA-modified magnetic beads for separation.The limit of detection was 12 cfu/mL for S. typhimurium with a linear range from 10(2)-10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1084 297296422018Design and fabrication of an electrochemical aptasensor using Au nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite for rapid and sensitive detection of Staphylococcus aureusStaphylococcus aureus aptamerTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC46N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an impedimetric aptasensor using AuNPs/CNPs/CNFs nanocomposites for the rapid detection of S. aureus.Exhibited a wide linear dynamic range 1.2 × 10(1) to 1.2 × 10(8) CFU/mL with a LOD of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1093 303505642018Aptamer-Based TB Antigen Tests for the Rapid Diagnosis of Pulmonary Tuberculosis: Potential Utility in Screening for TuberculosisH63 SL-2-M6AGGGCTTTTTTTTTTTTTAGTTCGTTTG28N/AssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenDeveloped an Aptamer ALISA and an electrochemical sensor (ECS) for the direct detection of HspX in sputum.Aptamer ALISA demonstrated excellent sensitivity of 94.1% (95% CI, 86.8–98%) and specificity of 100% (95% CI, 98.4–100%). Aptamer-based ECS detected HspX at ∼13 pM.N/AN/AN/ABiosensorN/AALISA: 5′-Biotinylated and ECS: 5'-Methylene Blue (MB) and 3'-ThiolatedN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1094 304138942018An impedimetric aptasensor for Shigella dysenteriae using a gold nanoparticle-modified glassy carbon electrodeAptamerATAGGAGTCACGACGACCAGAACGGAACTAGCGTTTAAATGCCAGGACTGAAGTAGGCAGGGATTAGTCAAGAGGTAGACGCACATA87N/AssDNAShigella Dysenteriae (PTCC 1188)Outer membrane protein (OMP)Developed an impedimetric aptasensor using GCE modified with AuNPs for the detection of S. dysenteriae.Display linear dynamic range that extends from 10(1) to 10(6) CFU/mL and a limit of detection of 100 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1121 301871422018Colorimetric aptasensor for Campylobacter jejuni cells by exploiting the peroxidase like activity of Au@Pd nanoparticlesaptamerGCAAGATCTCCGAGATATCGTGCTGGGGGGTGGTTTGTTTGGGTCGGTTGTTTTGGTTGGGCTGCAGGTAATACGTATACT81N/AssDNACampylobacter JejuniSurface proteinDevelop a colorimetric aptasensor based on an aptamer and Au@Pd NPs peroxidase for the determination of C. jejuni in the milk sample.The Limit of Detection (LOD) achieved was 10 CFU/mL in phosphate-buffered saline and 100 CFU/mL in milk samples with a linear range from 10 to 10(6) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1122 296494752018Use of DNA aptamer for sandwich type detection of Listeria monocytogenesLM6-116AGTATACGTATTACCTGCAGCTACTCGTTATTTCGTAGCACTTTTCCCCACCACCTTGGTGCGATATCTCGGAGATCTTGC81N/AssDNAListeria Monocytogenes (ATCC 19115)Surface proteinDevelop a two-site binding sandwich assay for capture and detection of L. monocytogenes.Display LOD of 2.5 CFU L. monocytogenes in 500 μl buffer and 10 CFU/25 g turkey deli meat sample.N/AN/A74.4 ± 52.69 nMBiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1123 294140892018A sensitive Potentiometric resolved ratiometric Photoelectrochemical aptasensor for Escherichia coli detection fabricated with non-metallic nanomaterialsE. coli aptamerGCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli)Surface proteinDevelops a sensitive potentiometric resolved ratiometric photoelectrochemical aptasensor for the detection of E. coli.Achieved an extremely low detection limit (LOD) of 0.66 cfu/mL and showed a wide linear range (2.9 cfu/mL to 2.9 × 10(6) cfu/mL).N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1124 298706922018Carbon nanotube-based aptasensor for sensitive electrochemical detection of whole-cell SalmonellaSalmonella aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an amino-modified aptasensor using MWCNTs-deposited ITO electrode for the detection of Salmonella.It possesses a linear range from 10 mV/s to 90 mV /s, and the detection limit was 5.5 × 10(1) cfu/mL and 6.7 × 10(1) cfu/mL for S. Enteritidis and S. Typhimurium, respectively.N/AN/AN/ABiosensorN/AAmidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1125 334186732018Fast and Sensitive Detection of Salmonella in Milk Samples Using Aptamer-Functionalized Magnetic Silica Solid Phase and MCM-41-Aptamer Gate SystemS. enterica specific aptamerTATGGCGGCGTCACCCGACGGGGACTT27N/AssDNASalmonella EntericaWhole cellDeveloped an aptamer-gated MCM-41 silica system (Fe3O4@SiO2@pGMA and MCM-41 particles) to detect S. enterica in food samples.The linear range is from 2000 CFU/mL to 104 CFU/mL in milk, with the LOD of 2336 cells.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1150 295503462018An aptasensor for staphylococcus aureus based on nicking enzyme amplification reaction and rolling circle amplificationAptamerTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA chemiluminescence aptasensor for S. aureus detection based on aptamer recognition and the DNA amplification cycle (NEAR-RCA) was developed.Detect S. aureus specifically with a good linear correlation at 5-10(4) CFU/mL and limit of detection (LoD) of 5 CFU/mL.N/AN/A210.70 ± 135.91 nMBiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1151 297773092018Fluorometric determination of Vibrio parahaemolyticus using an F0F1-ATPase-based aptamer and labeled chromatophoresV. parahaemolyticus aptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped an F0F1-ATPase-based aptasensor for the fluorometric determination of V. parahaemolyticus.The relative fluorescence varies linearly over the 15 to 1.5 × 10(6) cfu/mL Vibrio parahaemolyticus concentration range, and the limit of detection is 15 cfu/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1180 322548372018Sensitive and specific detection of clinical bacteria via vancomycin-modified Fe3O4@Au nanoparticles and aptamer-functionalized SERS tagsSa1GCAATGGTACGGTACTTCCACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDevelop a dual-recognition SERS platform using vancomycin-modified Fe3O4@Au magnetic nanoparticles (Fe3O4@Au-Van MNPs) for the detection of E. coli and S. aureus.The LOD of the platform was 20 cells/mL for S. aureus, with a capture efficiency of up to 88.89% within 15 min.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6) and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1181 322548372018Sensitive and specific detection of clinical bacteria via vancomycin-modified Fe3O4@Au nanoparticles and aptamer-functionalized SERS tagsE1GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli)Whole cellDevelop a dual-recognition SERS platform using vancomycin-modified Fe3O4@Au magnetic nanoparticles (Fe3O4@Au-Van MNPs) for the detection of E. coli and S. aureus.The LOD of the platform was 50 cells/mL for E. coli, and capture efficiency reached 74.96% within 15 min.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6) and 5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1182 299016742018Aptamer immobilization on amino-functionalized metal-organic frameworks: an ultrasensitive platform for the electrochemical diagnostic of Escherichia coli O157:H7E.coli aptamerCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellElectrochemical biosensor based on amino-functionalized metal-organic frameworks (MOFs) for detection of Escherichia coli O157:H7.Display linear range of 2.1×10(1) to 2.1×10(7) CFU/mL with a LOQ of 21 CFU/mL and LOD of 2 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1188 288878142018Construction and application of an electrochemical biosensor based on an endotoxin aptamerEAQ2ATGAGAGCGTCGGTGTGGTATGCTCACCCCCTGCGGCGCCGTTACGCGGTCCTTGTGTAGGAGGGTGCGGAAGTA75N/AssDNAEscherichia Coli (E. Coli) 055:B5 (L2880)Lipopolysaccharide (LPS)An electrochemical aptasensor was developed using an aptamer assembled on a gold electrode surface using 3-mercaptopropionic acid (MPA) as an intermediate linker to detect endotoxin.N/AN/ABio-Layer Interferometry (BLI)32.5 nMBiosensorN/A5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1189 288878142018Construction and application of an electrochemical biosensor based on an endotoxin aptamerEAQ3ATGAGAGCGTCGGTGTGGTATCGCCGCGCTCGCCGATCGTTCCTCCCCCCCCGTCTGTAGGAGGGTGCGGAAGTA75N/AssDNAEscherichia Coli (E. Coli) 055:B5 (L2880)Lipopolysaccharide (LPS)An electrochemical aptasensor was developed using an aptamer assembled on a gold electrode surface using 3-mercaptopropionic acid (MPA) as an intermediate linker to detect endotoxin.Showed a low detection limit of 0.001 EU/mL and a linear range of 0.001–1 EU/mL.N/ABio-Layer Interferometry (BLI)22.9 nMBiosensorN/A5'-Amidation (NH₂) and 5'-BiotinylatedN/AThe biosensor maintained a steady EIS response value after 1 week of storage in the PBS (10 mM, pH 7.4) at 4°C, and the response value still occupied 90%.Best CandidateN/AN/A
ABdb_1190 290782012018Gold atomic cluster mediated electrochemical aptasensor for the detection of lipopolysaccharideLPS aptamerCTTCTGCCCGCCTCCTTCCTAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCAGGAGACGAGATAGGCGGACACT86N/AssDNAEscherichia Coli (E. Coli) O111:B4Lipopolysaccharide (LPS)Developed an aptamer immobilized gold atomic cluster mediated, ultrasensitive electrochemical biosensor (Apt/AuAC/Au) for LPS detection.Achieved a detection of LPS down to 7.94 × 10–21 M level with a wide dynamic range from 0.01 attomolar to 1 pM.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AThe Apt/AuAC/Au electrode offers excellent stability when stored at 4°C for 1 week, with 95.54% of the signal retained.N/AN/AN/A
ABdb_1191 304210382018Dual-aptamer-based voltammetric biosensor for the Mycobacterium tuberculosis antigen MPT64 by using a gold electrode modified with a peroxidase loaded composite consisting of gold nanoparticles and a Zr(IV)/terephthalate metal-organic frameworkAPT-ITGGGAGCTGATGTCGCATGGGTTTTGATCACATGA35N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinA sandwich-like dual-aptamer strategy employing an ultrasensitive aptasensor for the voltammetric determination of M. tuberculosis. antigen MPT64 in human serum.Achieved an ultrasensitive detection limit of 10 fg/mL and a wide linear response range from 0.02 to 1000 pg/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1192 304210382018Dual-aptamer-based voltammetric biosensor for the Mycobacterium tuberculosis antigen MPT64 by using a gold electrode modified with a peroxidase loaded composite consisting of gold nanoparticles and a Zr(IV)/terephthalate metal-organic frameworkAPT-IITTCGGGAATGATTATCAAATTTATGCCCTCTGAT34N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinA sandwich-like dual-aptamer strategy where two different aptamers are immobilized on a gold electrode to synergistically bind the target MPT64 proteinAchieved an ultrasensitive detection limit of 10 fg/mL and a wide linear response range from 0.02 to 1000 pg/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1193 299072942018New colorimetric aptasensor for rapid on-site detection of Campylobacter jejuni and Campylobacter coli in chicken carcass samplesONS-23TAACAAGGGACAGTAGACCAACAGGAAATCAAAGGCCGTGGGAA42N/AssDNACampylobacter Jejuni (ATCC 33560, NCTC11168, 2 human isolates, and 3 food isolates)Whole cellA 2-stage label-free aptasensing platform was developed to detect C. jejuni and C. coli.Detection limit (LOD) of 7.2×10^5 CFU/mL and linear range from 10(5) to 10(8) CFU/mL for C. jejuni.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1194 299072942018New colorimetric aptasensor for rapid on-site detection of Campylobacter jejuni and Campylobacter coli in chicken carcass samplesONS-23TAACAAGGGACAGTAGACCAACAGGAAATCAAAGGCCGTGGGAA42N/AssDNACampylobacter Coli (ATCC 33559 and 3 food isolates)Whole cellAptamers normally adsorb to gold nanoparticles (AuNPs) to protect them from salt-induced aggregation.Detection limit (LOD) of 5.6×10^5 CFU/mL with a strong correlation (from 10(5) to 10(8) CFU/mL) for C. coli.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1195 300191372018Aptamer based voltammetric biosensor for Mycobacterium tuberculosis antigen ESAT-6 using a nanohybrid material composed of reduced graphene oxide and a metal-organic frameworkESAT-6 binding aptamer (EBA)GGGAGCTCAGAATAAACGCTCAACCTCCCTGGGTCACCATAGACTCCATCTAAGATGCTTCGACATGAGGCCCGGATC78N/AssDNAMycobacterium Tuberculosis (M.Tb.)ESAT-6 (6-kDa early secretory antigenic target)Develop a voltammetric aptasensor employing P-MOF-rGO nanohybrid as the sensitive sensing platform for ultrasensitive detection of ESAT-6.Linear range: 1.0×10(−4) to 2.0×10(2) ng/mL and Limit of Detection (LOD): 3.3×10(−5) ng/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/ACompared with the initial current response, the aptsensor response decreased only about 3.5% after 50 cycles’ continuous CV scans.N/AN/AN/A
ABdb_1196 300810312018Label-free aptasensors based on fluorescent screening assays for the detection of Salmonella typhimuriumS. typhimurium binding Aptamer (Apt1)CCAAAGGCTACGCGTTAACGTGGTGTTGG29N/AssDNASalmonella Typhimurium (S. Typhimurium) (MTCC 3232)Whole cellA label-free fluorescent aptasensor was developed for the detection of Salmonella typhimurium using SYBR Green I.Achieved a linear range of approximately 1530-96938 CFU/mL and a detection limit of 733 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1197 300810312018Label-free aptasensors based on fluorescent screening assays for the detection of Salmonella typhimuriumS. typhimurium binding Aptamer (Apt2)ATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (MTCC 3232)Whole cellAn aptasensor based on FRET between Rhodamine B and gold nanoparticles for the detection of Salmonella typhimurium was developed.Achieved concentrations ranging from 1530 to 96938 CFU/mL, with a detection limit of 464 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1198 303595192018Ω-Shaped Fiber-Optic Probe-Based Localized Surface Plasmon Resonance Biosensor for Real-Time Detection of Salmonella TyphimuriumAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-immobilized Ω-shaped fiber-optic localized surface plasmon resonance (FOLSPR) biosensor for S. Typhimurium detection.Achieved high detection sensitivity for S. Typhimurium down to 128 CFU/mL within a linear range from 5 × 10(2) to 1 × 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1199 https://doi.org/10.1016/j.snb.2017.08.1602018Nanoporous gold as a suitable substrate for preparation of a new sensitive electrochemical aptasensor for detection of Salmonella typhimuriumAnti-S. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a label-free electrochemical aptasensor based on nanoporous gold (NPG/Au/GCE) for the detection of Salmonella typhimurium.Capable of detecting S. typhimurium in a wide linear dynamic range 6.5 × 10(2) to 6.5 × 10(8) CFU/mL with a LOD of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1200 295338182018Culture-free, highly sensitive, quantitative detection of bacteria from minimally processed samples using fluorescence imaging by smartphoneS. aureus–specific aptamer (Sap)TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (USA300)Surface proteinDeveloped a smartphone-based detection using aptamer-functionalized fluorescent magnetic nanoparticles (FMNPs) for Staphylococcus aureus.Showed a minimum detectable concentration as low as 10 cfu/ml in a peanut milk sample within 10 min.N/AN/A3.03 nMBiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1201 300014872018Magnetism-Resolved Separation and Fluorescence Quantification for Near-Simultaneous Detection of Multiple PathogensE. coli aptamerATCCGTCACACCTGCTCTATCAAATGTGCAGATATCAAGACGATTTGTACAAGATGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O157:H7 (CICC 21530)Whole cellAptamer-modified fluorescent-magnetic multifunctional nanoprobes (apt-FMNPs) for near-simultaneous detection of multiple pathogens.The detection limits were 100 cfu/mL in milk, 80 cfu/mL in human serum, and 80 cfu/mL in human urine for E. coli.N/AN/AN/ABiosensorN/A5'-Biotinylated and 3'-Cyanine3 (Cy3) LabeledBacterial viability remained about 100% during incubation with apt-FMNPs nanoprobes at successive incubation time points of 0, 0.5, 1, 3, 6, 12, and 18 h.N/AN/AN/AN/A
ABdb_1202 300014872018Magnetism-Resolved Separation and Fluorescence Quantification for Near-Simultaneous Detection of Multiple PathogensS. typ aptamerATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellAptamer-modified fluorescent-magnetic multifunctional nanoprobes (apt-FMNPs) for near-simultaneous detection of multiple pathogens.The detection limits of 150 cfu/mL in milk, 120 cfu/mL in human serum, and 120 cfu/mL in human urine for S. typ.N/AN/AN/ABiosensorN/A5'-Biotinylated and 3'-FAM LabeledBacterial viability remained about 100% during incubation with apt-FMNPs nanoprobes at successive incubation time points of 0, 0.5, 1, 3, 6, 12, and 18 h.N/AN/AN/AN/A
ABdb_1224 306374362019Aptamer-mediated colorimetric and electrochemical detection of Pseudomonas aeruginosa utilizing peroxidase-mimic activity of gold NanoZymeF23CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellAptamer-mediated tunable NanoZyme colorimetric and electrochemical sensors for the detection of Pseudomonas aeruginosa.Possess a detection limit of the electrochemical sensor of ~ 60 CFU/mL in water within 10 min.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1225 312163062019Aptamer-based fluorometric determination of Salmonella Typhimurium using Fe3O4 magnetic separation and CdTe quantum dotsAptamer (ssDNA1)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Outer membrane protein (OMP)Develop an aptamer-based (Apt-MNPs) fluorescence assay for the determination of Salmonella Typhimurium.Exhibit a linear range of 10 to 10(10) cfu/mL with LOD of 1 cfu/mL within 2 h, and is successfully applied to the analysis of spiked food samples with good recoveries from 90% to 105%.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_1227 308778872019A new aptamer/polyadenylated DNA interdigitated gold electrode piezoelectric sensor for rapid detection of Pseudomonas aeruginosaP. aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellDeveloped a piezoelectric sensor (Au IDE-MSPQC) based on magnetic bead/aptamer/polyadenylated-DNA for the detection of P. aeruginosa.The limits of detection (LOD) of the method were as low as 9 CFU/mL in buffer and 52 CFU/mL in a simulated blood sample.N/AN/AN/ABiosensorN/A5'-Biotinylated (Biotin-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1228 311835762019Surface-enhanced Raman spectroscopic single step detection of Vibrio parahaemolyticus using gold coated polydimethylsiloxane as the active substrate and aptamer modified gold nanoparticlesV.P. AptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellAptamer-based SERS method using Au-PDMS film for the detection of V. parahaemolyticus.The detection limit is 12 cfu/mL with a wide linear range from 1.2 × 10(2) to 1.2 × 10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1229 314724132019Vertical capacitance aptasensors for real-time monitoring of bacterial growth and antibiotic susceptibility in bloodE. coli aptamerGCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellVertical-type aptamer-functionalized sensor (aptasensor) for monitoring bacterial growth and antibiotic susceptibility in blood in real-time.Detect bacteria growth in blood at 10(0)-10(3) CFU/mL in real-time within 12 hours, and the MIC for E. coli was approximately 0.1 and 1 μg/mL for gentamicin and amikacin, respectively.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1230 314724132019Vertical capacitance aptasensors for real-time monitoring of bacterial growth and antibiotic susceptibility in bloodP. aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellVertical-type aptamer-functionalized sensor (aptasensor) for monitoring bacterial growth and antibiotic susceptibility in blood in real-time.Detect bacteria growth in blood at 10(0)-10(3) CFU/mL in real-time within 12 hours and the MIC for S. aureus was approximately 1 and 0.1 μg/mL for gentamicin and amikacin, respectively,N/AN/AN/ABiosensorN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_1231 314724132019Vertical capacitance aptasensors for real-time monitoring of bacterial growth and antibiotic susceptibility in bloodS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellVertical-type aptamer-functionalized sensor (aptasensor) for monitoring bacterial growth and antibiotic susceptibility in blood in real-time.Detect bacteria growth in blood at 10(0)-10(3) CFU/mL in real-time within 12 hours and the MIC for P. aeruginosa was approximately 1 μg/mL for both gentamicin and amikacin.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1232 https:doi.org10.1039C9AY01509D2019A nano-sized chitosan particle based electrochemical aptasensor for sensitive detection of P. aeruginosaP.aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellDevelop an aptasensor based on a glassy carbon electrode (GCE) modified with nano-sized chitosan particles (NCs) for sensitive and simultaneous detection of P. aeruginosa.Displayed a low detection limit of 3 CFU/mL, wide linearity of 10(1)–10(7) CFU/mL and a recovery rate from 93.2% to 124%.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1234 313621882019Dual-aptamers labeled polydopamine-polyethyleneimine copolymer dots assisted engineering a fluorescence biosensor for sensitive detection of Pseudomonas aeruginosa in food samplesP. aeruginosa aptamer1CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 15442)Whole cellA dual aptamer labelled PDA-PEI copolymer dots-based biosensor for detection and quantification of P. aeruginosa.The LOD of P. aeruginosa is as low as 1 cfu/mL with a linear range from 10(1)–10(7) cfu/mL in 1.5h.N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1235 313621882019Dual-aptamers labeled polydopamine-polyethyleneimine copolymer dots assisted engineering a fluorescence biosensor for sensitive detection of Pseudomonas aeruginosa in food samplesP. aeruginosa aptamer2ATGCACTCTTCTATCGGTAGTTGAGGGTGCGG32N/AssDNAPseudomonas Aeruginosa (ATCC 15442)Whole cellA dual aptamer labelled PDA-PEI copolymer dots-based biosensor for detection and quantification of P. aeruginosa.The LOD of P. aeruginosa is as low as 1 cfu/mL with a linear range from 10(1)–10(7) cfu/mL in 1.5h.N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1236 312791712019A novel enzyme-free electrochemical biosensor for rapid detection of Pseudomonas aeruginosa based on high catalytic Cu-ZrMOF and conductive Super PP-aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellDevelop a Cu-ZrMOF@Aptamer@DNA nanocomposite-based enzyme-free electrochemical biosensor to detect P. aeruginosa.Exhibit a wide linearity range of 10-10(6) CFU/mL and a low limit of detection of 2 CFU/mL within 120 min.N/AN/AN/ABiosensorN/A5'-PhosphateN/AThe biosensor had acceptable storage stability (98.1% and 83.9%) within 7 days of storage at 4°C.N/AN/AN/A
ABdb_1237 316558992019Impedimetric aptasensor for Pseudomonas aeruginosa by using a glassy carbon electrode modified with silver nanoparticlesP.aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellDevelop an aptasensor based on an immobilised NH2-aptamer that was covalently attached to the AgNP/GCE surface for the detection of P. aeruginosa.The impedance increases on going from 10(2) to 10(7) CFU/mL concentrations of P. aeruginosa, and the detection limit is 33 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1245 308256572019A reduced graphene oxide-titanium dioxide nanocomposite based electrochemical aptasensor for rapid and sensitive detection of Salmonella entericaS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a rGO-TiO2 electrochemical aptasensor for the detection of Salmonella bacteria.Exhibited high sensitivity with a wide detection range (10(8) to 10(1) cfu/mL), a low detection limit of 10(1) cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AShowed good durability up to 21 days with a 10% decrease in signal after 30 days of non-use.N/AN/AN/A
ABdb_1246 https://doi.org/10.1016/j.foodcont.2019.1068062019A competitive enzyme linked aptasensor with rolling circle amplification (ELARCA) assay for colorimetric detection of Listeria monocytogenesAptamerTATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT48N/AssDNAListeria Monocytogenes (CMCC 54007)Whole cellDeveloped an enzyme-linked aptasensor with rolling circle amplification (ELARCA) assay for the detection of L. monocytogenes.Showed a limit of detection (LOD) of 4.6 × 10(2) CFU/mL in pure culture, and in fresh lettuce showed an LOD of 6.1 × 10(3) CFU/g.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1247 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA27CCTTCTAACAGAATGTTGTTAGATAGC27N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.Detection limits (3σ/S) obtained within 30 minutes using LA27 with respect to LPS, Pa-LPS, and Ecoli-LPS were 38.7, 88.0, and 154 ng/mL, respectively.N/AIsothermal Titration Calorimetry (ITC)46.2 ± 9.5 nMBiosensorN/A5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1248 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA80ATAGGAGTCACGACGACCAGCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTCTATGTGCGTCTACCTCTTGA80N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.Detection limits (3σ/S) obtained using LA80 with respect to LPS, Pa-LPS, and Ecoli-LPS were 0.185, 2.56 and 2.82 μg/mL, respectively.N/AIsothermal Titration Calorimetry (ITC)102 ± 17 nMBiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1249 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA60TCACGACGACCAGCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTCTATGTGC60N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1250 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA54CGACGACCAGCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTCTATG54N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1251 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA48CGACCAGCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTCT48N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1252 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA40CCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGCTGGTC40N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1253 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA36GCCTTCTAACAGAATGTTGTTAGATAGCTTTGAAGC36N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.LA36 has good specificity.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1254 307711022019Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamersLA22TTCTAACAGAATGTTGTTAGAT22N/AssDNASalmonella Typhimurium (S. Typhimurium), Escherichia Coli (E. Coli) 055:B5 and Pseudomonas Aeruginosa 10Lipopolysaccharide (LPS)Developed an aptamer-based graphene oxide-based fluorescence polarization assay to detect LPSs from S. typhimurium, P. aeruginosa 10 and E. coli 055:B5.N/AN/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1255 306086832019Visualized Detection of Vibrio parahaemolyticus in Food Samples Using Dual-Functional Aptamers and Cut-Assisted Rolling Circle AmplificationA-AptTACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCAAAAAAAAAA71N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellA biosensor using Dual-Apt and cut-assisted rolling circle amplification (CA-RCA) for rapid and visualized detection of Vibrio parahaemolyticus.Shows a good linear correlation in the range from 10(6) to 10 CFU/mL and LOD as low as 10 CFU/mL (g) in real food samples.N/AN/AN/ABiosensorN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1256 306086832019Visualized Detection of Vibrio parahaemolyticus in Food Samples Using Dual-Functional Aptamers and Cut-Assisted Rolling Circle AmplificationD-AptCCTCAGCATAAGCATGAATTGACCAACCTAAACTTATTCATTTTCCAGCACCTCTAATATTACTGGCGCAGTGCACCCACCCACCCACCC90N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellA biosensor using Dual-Apt and cut-assisted rolling circle amplification (CA-RCA) for rapid and visualized detection of Vibrio parahaemolyticus.Shows a good linear correlation in the range from 10(6) to 10 CFU/mL and LOD as low as 10 CFU/mL (g) in real food samples.N/AN/AN/ABiosensorN/A5'-Phosphate (3'-end phosphorylation)N/AN/AN/AN/AN/A
ABdb_1257 313077212019Dual-recognition surface-enhanced Raman scattering(SERS)biosensor for pathogenic bacteria detection by using vancomycin-SERS tags and aptamer-Fe3O4@AuS. aureus-aptamerGCAATGGTACGGTACTTCCACTTAGGTCGAGGTTAGTTTGTCTTGCTGGCGCATCCACTGAGCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a dual-recognition SERS biosensor using Vancomycin-Au@MBA and aptamer-Fe3O4@Au for the detection of pathogenic bacteria.A detection limit of 3 cells/mL with a wide dynamic linear range from 10 to 10(7) cells/mL can be achieved within 50 min.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1274 306375072019Colorimetric detection and typing of E. coli lipopolysaccharides based on a dual aptamer-functionalized gold nanoparticle probeSpecific aptamer (S-probe)ATCCGTCACCCCTGCTCTCGTCGCTATGAAGTAACAAAGATAGGAGCAATCGGGTGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O111:B4Lipopolysaccharide (LPS)Develop a dual aptamer-based colorimetric method for the identification of E.coli LPS.Can detect LPS in concentrations between 2.5 and 20 μg/mL and with a 1 μg/mL detection limit.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1275 306375072019Colorimetric detection and typing of E. coli lipopolysaccharides based on a dual aptamer-functionalized gold nanoparticle probeGeneral aptamer (G-probe)AGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT39N/AssDNAEscherichia Coli (E. Coli) O111:B4 and O111:B5EthanolamineDevelop a dual aptamer-based colorimetric method for the identification of E.coli LPS.Can detect LPS in concentrations between 2.5 and 20 μg/mL and with a 1 μg/mL detection limit.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1276 303521982019A novel aptamer-based test for the rapid and accurate diagnosis of pleural tuberculosisH63 SL-2 M6AGGGCTTTTTTTTTTTTTAGTTCGTTTG28N/AssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenAptamer-Linked Immobilized Sorbent Assay (ALISA) was developed to detect pleural TB (pTB).Detects pTB with ~93% sensitivity and ~98% specificity.N/AN/AN/ADiagnosticN/A5'-BiotinylatedN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1277 309886112019Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitisH63 SL-2 M6AGGGCTTTTTTTTTTTTTAGTTCGTTTG28N/AssDNAMycobacterium Tuberculosis (M.Tb.)HspX antigenDeveloped a structural switching electrochemical aptasensor using methylene blue (MB) and an aptamer for the detection of HspX in CSF samples for the diagnosis of TBM.Can detect as low as 10 pg HspX in CSF with ~95% sensitivity and ~97.5% specificity in ≤30 minutes.N/AN/AN/ADiagnosticN/A5'-Methylene Blue (MB) and 3'-C6 thiol modificationN/AN/AN/AN/AIndian Patent application no. 201611001550
ABdb_1278 313808722019A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamersSA31TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA45N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDevelop a transcription aptasensor by using a light-up RNA aptamer for culture-free detection of intact foodborne pathogens.The dynamic range was 10(2)-10(6) CFU/mL, and the LOD of the transcription aptasensor was estimated to be 77.0 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1279 316173472019Ultrasensitive Fluorometric Angling Determination of Staphylococcus aureus in Vitro and Fluorescence Imaging in Vivo Using Carbon Dots with Full-Color EmissionS. aureus aptamer (Apt)GCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellAn ultrasensitive magnetic fluorescence aptasensor was designed using Fe3O4/CD for the separation and detection of S. aureus.The FRET-based aptasensor achieved a wide linear range of 50–10(7) CFU/mL and a detection limit of 8 CFU/mL.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1280 310361832019Rapid and label-free detection of Brucella melitensis in milk and milk products using an aptasensorB. melitensis aptamerGAGAGTAAAGGCCATCGGCGGCCATTTATGTTGTACCC38N/AssDNABrucella MelitensisWhole cellA QCM-aptasensor [Fe3O4 @SiO2 @p(PEG-MA-GMA)] was developed for the determination of B. melitensis from food samples.The detection limits of the QCM aptasensor were in the range 1.0(2)–1.0(7) CFU/mL, with recoveries up to 79% and LOD of 100 CFU/mL.15N/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1281 317180452019Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using AcoustophoresisGN6 aptamerATACCAGCTTATTCAATTGGGTGAGGGGGGGTTCACAACGTTAAAGATAGACGGGGGAAGATAGTAAGTGCAATCT76N/AssDNAGram-negative BacteriaWhole cellDevelop aptamer-modified microbeads and an acoustophoresis method for capturing and displacing gram-negative bacteria.It achieved high recovery (up to 98%) and high purity (up to 99.5%).N/AN/AN/ADetectionN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1324 317328072019A glassy carbon electrode modified with reduced graphene oxide and gold nanoparticles for electrochemical aptasensing of lipopolysaccharides from Escherichia coli bacteriaLPS AptamerCTTCTGCCCGCCTCCTTCCTAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCAGGAGACGAGATAGGCGGACACT86N/AssDNAEscherichia Coli (E. Coli) 055:B5Lipopolysaccharide (LPS)An electrochemical aptasensor immobilized on a GCE modified with reduced graphene oxide and gold nanoparticles (RGO/AuNPs) was developed for the voltammetric determination of LPS from E. coli 055:B5.Limit of Detection (LOD) of 1 fg/mL (using Mg/CQD media) or 30 fg/mL (using hexacyanoferrate).N/AN/A12 nMBiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1325 312021032019A sandwich-type electrochemical aptasensor for Mycobacterium tuberculosis MPT64 antigen detection using C60NPs decorated N-CNTs/GO nanocomposite coupled with conductive PEI-functionalized metal-organic frameworkMPT64 antigen aptamer Ⅰ (MAA Ⅰ)TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA35N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDevelop a sandwich-type electrochemical aptasensor using (C60NPs-N-CNTs/GO) for the detection of MPT64 antigen.Showed a wide linear range from 1 fg/mL to 1 ng/mL with a limit of detection (LOD) as low as 0.33 fg/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AOxidation current remains 94.5% of the initial level after 80 cycles, and peak current remains approximately 98.7%, 93.5%, and 90.6% of the initial peak current after 7, 14, and 21 days, respectively.N/AN/AN/A
ABdb_1326 312021032019A sandwich-type electrochemical aptasensor for Mycobacterium tuberculosis MPT64 antigen detection using C60NPs decorated N-CNTs/GO nanocomposite coupled with conductive PEI-functionalized metal-organic frameworkMPT64 antigen aptamer Ⅱ (MAA Ⅱ)TTCGGGAATGATTATCAAATTTATGCCCTCTGAT34N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDevelop a sandwich-type electrochemical aptasensor using (C60NPs-N-CNTs/GO) for the detection of MPT64 antigen.Showed a wide linear range from 1 fg/mL to 1 ng/mL with a limit of detection (LOD) as low as 0.33 fg/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AOxidation current remains 94.5% of the initial level after 80 cycles, and peak current remains approximately 98.7%, 93.5%, and 90.6% of the initial peak current after 7, 14, and 21 days, respectively.N/AN/AN/A
ABdb_1327 314469522019Aptamer-based SERS biosensor for whole cell analytical detection of E. coli O157:H7a-aptamerATCAAATGTGCAGATATCAAGACGATTTGTACAAGATCCATGCTGAGGTGGTCATAGCTGATCCTACC68N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellSERS-based aptasensor using 4-aminothiophenol-gold nanoparticle complexes was developed for the detection of E. coli O157:H7.Low concentrations of E. coli O157:H7 were detected and quantified within 20 min in both pure culture (∼10(1) CFU/mL) and ground beef samples (∼10(2) CFU/mL), and a linear range from 10(2) to 10(6) CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1328 https://doi.org/10.3390/app90202952019Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice ExtractAptamer (Seq.1)CAGCTCAGAAGCTTGATCCTACCAGTAGACTTTCAACTTTACTGCCATCGTGTGCCCTAAGACTCGAAGTCGTGCATCTG80N/AssDNAEscherichia Coli (E. Coli) (CICC 10372)Whole cellDeveloped an aptamer-based electrochemical biosensor for the rapid detection of E. coli in liquorice extract.Concentrations ranging from 5.0 × 10^2 CFU/mL to 5.0 × 10^7 CFU/mL exhibited a linear trend, with a detection limit of 80 CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1329 314105762019Electrochemical determination of Salmonella typhimurium by using aptamer-loaded gold nanoparticles and a composite prepared from a metal-organic framework (type UiO-67) and grapheneAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellAn aptamer-based assay based on a metal-organic framework-graphene composite of type UiO-67/GR and an aptamer-gold nanoparticles-horseradish peroxidase (Apt-AuNP-HRP) conjugate is described for the determination of S.typhimurium.Linear detection range from 2 × 10(1) – 2 × 10(8) cfu/mL and a lower detection limit of 5 cfu/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/A91.3% of the original current is retained after storage at 4°C for 14 days with the modified electrodes, allowing monitoring of 2 × 10(5) cfu/mL S. typhimurium.N/AN/AN/A
ABdb_1330 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens10A-E1AAAAAAAAAA-TTTTT-ATCAAATGTGCAGATATCAAGACGATTTGTACAAGAT52N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.The detection limit of the current SPR aptasensor is 1 x 10(5) CFU/mL for E. coli.N/AN/AN/ABiosensorN/A5'-PolyA (10A) and Five thymine (5T)N/AN/ABest CandidateN/AN/A
ABdb_1331 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens10A-E2AAAAAAAAAA-TTTTT-CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG57N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (10A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1332 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens10A-S1AAAAAAAAAA-TTTTT-TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC77N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.The detection limit of the current SPR aptasensors is 1 x 10(6) CFU/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-PolyA (10A) and Five thymine (5T)N/AN/ABest CandidateN/AN/A
ABdb_1333 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens10A-S2AAAAAAAAAA-TTTTT-GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA103N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (10A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1334 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens30A-E1AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-TTTTT-ATCAAATGTGCAGATATCAAGACGATTTGTACAAGAT72N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (30A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1335 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens30A-E2AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-TTTTT-CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG77N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (30A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1336 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens30A-S1AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-TTTTT-TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC97N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (30A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1337 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens30A-S2AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-TTTTT-GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA123N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (30A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1338 306220362019An electrochemical aptasensor for staphylococcal enterotoxin B detection based on reduced graphene oxide and gold nano-urchinsAptamer SEBTGCAGGATCCGGTATCCGTGCACACACACCCAACAACCAGCTGCCGCACCGGAGGAATTCTCGT64N/AssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin B (SEB)An electrochemical aptasensor was developed using a screen-printed electrode modified with reduced graphene oxide (rGO) and gold nano-urchins (AuNUs) for the detection of SEB.A wide linear range of 5.0–500.0 fM was observed, with a detection limit of 0.21 fM.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1339 305220852019A label-free impedimetric aptasensor for the detection of Bacillus anthracis spore simulantBAS-6RATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGATGGTGTTGGCTCCCGTAT71N/AssDNABacillus Cereus (ATCC 11778 and ATCC 14579)B. anthracis spore simulant (B. cereus spore 14579)Developed an impedimetric DNA-based aptasensor based on an aptamer immolized on a gold screen-printed electrode for a single-step detection of B. cereus spores.Spores were detected with a linear range between 10(4) CFU/ml and 5 × 10(6) CFU/ml and a detection limit of 3 × 10(3) CFU/ml.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1340 300786222019Electrochemical aptasensor using optimized surface chemistry for the detection of Mycobacterium tuberculosis secreted protein MPT64 in human serumAptamerTCACTTCAAATGTGCGCTTC-GTCAACAGTCCAGTTGGGAGGTCGCTTATAGCTGCTTGTC-CGTCAAAACAGGGGGTAGAA80N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDeveloped an electrochemical aptasensor using thiol PEGylated aptamer HS-(CH2)6-OP(O)2O-(CH2CH2O)6-TTTTT-aptamer and 6-mercaptohexanol for the detection of MPT64.A spiked human serum sample displays a limit of detection of 81 pM in 30 minutes with the long linker aptamer/MCH.N/AN/A8.92 nMBiosensorN/AHS-(CH2)6-OP(O)2O-(CH2CH2O)6-5'-TTTTT-aptamer-3' and HS-(CH2)6-5'-TTTTT-aptamer-3'N/AN/AN/AN/AN/A
ABdb_1344 310331562019Inkjet Printed Nanopatterned Aptamer-Based Sensors for Improved Optical Detection of Foodborne Pathogensa-aptamerATCAAATGTGCAGATATCAAGACGATTTGTACAAGATCCATGCTGAGGTGGTCATAGCTGATCCTACC68N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDeveloped an inkjet-printed nanopatterned aptamer-based sensor for optical detection of E. coli O157:H7.Display a LOD of 25 CFU/mL in pure culture and 233 CFU/mL in ground beef.N/ABio-Layer Interferometry (BLI)N/ABiosensorN/A5'-Carboxy and 5'-Biotin-TEGN/AN/AN/AN/AN/A
ABdb_1345 307974662019Aptamer surface functionalization of microfluidic devices using dendrimers as multi-handled templates and its application in sensitive detections of foodborne pathogenic bacteriaE. coli O157:H7 aptamerCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellA microfluidic system using a dendrimer-aptamer modified surface was developed for sensitive detection of E. coli O157:H7.The G7-apatamer modified detection system achieved a low limit of detection of 10(2) cells/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) and 3'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1347 311964182019Functional chimera aptamer and molecular beacon based fluorescent detection of Staphylococcus aureus with strand displacement-target recycling amplificationAptamerCACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT48N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a fluorescent detection of S. aureus based on a functional chimaera and a molecular beacon.Display a broad concentration range from 80 CFU/mL to 8 × 10(6) CFU/mL and the detection limit of 39 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1348 313182032019Engineering DNA-Nanozyme Interfaces for Rapid Detection of Dental BacteriaS. mutans-binding aptamerATACTATCGCATTCCTTCCGAGGGGGGAGGGGGGGGTGGGGGTCGGT47N/AssDNAStreptococcus Mutans (ATCC 25175)Whole cellDeveloped a DNA-engineered nanozyme interface using aptamers for the detection of dental bacteria.The detection limit is 12 CFU/mL, and the linear range is 0 to 10(9) CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1350 323331132020Inhibitory effects of aptamer targeted teicoplanin encapsulated PLGA nanoparticles for Staphylococcus aureus strainsSA20GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus) susceptible strains and MRSAWhole cellAptamer-PLGA nanoparticles (Apt-teicoplanin-PLGA NPs) for the delivery of teicoplanin antibiotic.MICs of teicoplanin decreased by 32- and 64-fold for susceptible strains and MRSA strains, respectively.N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1351 329810122020Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamerSELEX 10 colony 5CCGGAATTCCTAATACGACTCTACAAAAAGTCGTGGTCCAGTTGCCATGTGTAAAACTGTGTATTGAAAACGCGGCCGCGG81N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellInterfering with the biofilm formation via abolishing the motility and quorum sensing.Exhibited the highest biofilm inhibition towards EPEC K1.1 shown by lowest OD value of 0.126.N/AN/AN/ATherapeuticsN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_1352 329810122020Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamerSELEX 8 colony 1CCGGAATTCCTAATACGACTCTGCGGACTGTATGCGGTACGGTCGAAAATAGTGAAGGTGCTATTGAAAACGCGGCCGCGG81N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellInterfering with the biofilm formation via abolishing the motility and quorum sensing.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1353 329810122020Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamerSELEX 8 colony 4CCGGAATTCCTAATACGACTCACTACGCACGGCGCGAGTAAATCGATCATGGTACTGTGGCTATTGAAAACGCGGCCGCGG81N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellInterfering with the biofilm formation via abolishing the motility and quorum sensing.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1354 329810122020Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamerSELEX 8 colony 7CCGGAATTCCTAATACGACTCGGTTCCGGTAAGATTAGATCATAACGTATGGCTAGCGCCATATTGAAAACGCGGCCGCGG81N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellInterfering with the biofilm formation via abolishing the motility and quorum sensing.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1355 329810122020Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamerSELEX 9 colony 3CCGGAATTCCTAATACGACTCGAAAACGTACCACTGGGATGGGTTGTGGGAGAGGGCCAGGTATTGAAAACGCGGCCGCGG81N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellInterfering with the biofilm formation via abolishing the motility and quorum sensing.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1356 329810122020Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamerSELEX 10 colony 10CCGGAATTCCTAATACGACTCATCCAGCAGATGTGCGCGGGTTGGTGGGGGAACGGTGGGATATTGAAAACGCGGCCGCGG81N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellInterfering with the biofilm formation via abolishing the motility and quorum sensing.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1369 327925732020Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolatesAptamer 1GCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (U433, U556-ESBL, B12327, U5307, U6267-ESBL)Whole cellElectrical AST (e-AST) chips composed of 60 aptamer-functionalized capacitance sensors to detect bacteria and measure the antibiotic susceptibility to 11 antibiotics at five different concentrations.The discrepancies between e-AST and BMD tests were estimated to be 2.20% mE, 0.38% ME, and 0.38%, which are lower than the FDA requirements (mE ≤ 10%, ME ≤ 3%, and VME ≤ 1.5%).N/AN/AN/ADiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_1370 327925732020Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolatesAptamer 2TACATGGTCAACCAAATTCTTGCAAATTCTGCATTCCTACTGT43N/AssDNAAcinetobacter Baumannii (P762, T800, R4197, R4299, R4356)Whole cellElectrical AST (e-AST) chips composed of 60 aptamer-functionalized capacitance sensors to detect bacteria and measure the antibiotic susceptibility to 11 antibiotics at five different concentrations.The discrepancies between e-AST and BMD tests were estimated to be 2.20% mE, 0.38% ME, and 0.38%, which are lower than the FDA requirements (mE ≤ 10%, ME ≤ 3%, and VME ≤ 1.5%).N/AN/AN/ADiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_1371 327925732020Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolatesAptamer 3N/AN/A5'-GCAATGGTACGGTACTTCC-(N45)-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAKlebsiella Pneumoniae (PAE1, PAE2, PAE3, PAE4, PAE5)Whole cellElectrical AST (e-AST) chips composed of 60 aptamer-functionalized capacitance sensors to detect bacteria and measure the antibiotic susceptibility to 11 antibiotics at five different concentrations.The discrepancies between e-AST and BMD tests were estimated to be 2.20% mE, 0.38% ME, and 0.38%, which are lower than the FDA requirements (mE ≤ 10%, ME ≤ 3%, and VME ≤ 1.5%).N/AN/AN/ADiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_1372 327925732020Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolatesAptamer 4CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (KP1-ESBL, KP2-ESBL, KP3, KP4-ESBL, KP5-ESBL)Whole cellElectrical AST (e-AST) chips composed of 60 aptamer-functionalized capacitance sensors to detect bacteria and measure the antibiotic susceptibility to 11 antibiotics at five different concentrations.The discrepancies between e-AST and BMD tests were estimated to be 2.20% mE, 0.38% ME, and 0.38%, which are lower than the FDA requirements (mE ≤ 10%, ME ≤ 3%, and VME ≤ 1.5%).N/AN/AN/ADiagnosticN/A3'-Thiolated (SH-(CH2)3)N/AN/AN/AN/AN/A
ABdb_1373 327925732020Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolatesAptamer 5GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (P101, T82, C970, R4308, R4319)Whole cellElectrical AST (e-AST) chips composed of 60 aptamer-functionalized capacitance sensors to detect bacteria and measure the antibiotic susceptibility to 11 antibiotics at five different concentrations.The discrepancies between e-AST and BMD tests were estimated to be 2.20% mE, 0.38% ME, and 0.38%, which are lower than the FDA requirements (mE ≤ 10%, ME ≤ 3%, and VME ≤ 1.5%).N/AN/AN/ADiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_1374 327925732020Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolatesAptamer 6ATCCAGAGTGACGCAGCACGACACGTTAGGTTGGTTAGGTTGGTTAGTTTCTTGTGGACACGGTGGCTTA70N/AssDNAEnterococcus Faecalis (R1238, U554, U5179, U4879, U5064)Whole cellElectrical AST (e-AST) chips composed of 60 aptamer-functionalized capacitance sensors to detect bacteria and measure the antibiotic susceptibility to 11 antibiotics at five different concentrations.The discrepancies between e-AST and BMD tests were estimated to be 2.20% mE, 0.38% ME, and 0.38%, which are lower than the FDA requirements (mE ≤ 10%, ME ≤ 3%, and VME ≤ 1.5%).N/AN/AN/ADiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_1375 324518002020Inhibition of Salmonella enteritidis biofilms by Salmonella invasion protein-targeting aptamerApt17TAGGGAAGAGAAGGACATATGAT-GCAATGGAACCGCTGAACGACCCTAGCATTATCAGTGTGG-TTGACTAGTACATGACCACTTGA86N/AssDNASalmonella Enteritidis (S. Enteritidis) TM 6 andTM 68Salmonella invasion proteinA (SipA)An aptamer targets the SipA protein to inhibit Salmonella biofilm formation by interfering with the T3SS.Co-incubation of Apt17 with ampicillin MIC/10 for 24 h inhibited the biofilms of S. enteritidis TM 6 and S. enteritidis TM 68 by 12.5% and 20.9% respectively.N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1376 327852022020Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver NanoclustersNC1TACTTCCGCACCCTCCTACA20N/AssDNAPseudomonas Aeruginosa (ATCC 10145)BiofilmDNA aptamer-enclosed silver nanoclusters (Ag-NC) were used to prevent biofilms.Potency of an aptamer-DNA enclosed Ag-NC with a decrease in the net Ld wrt control follows NC2 > NC1 > NC5 > positive control ≈ NC3 > NC6.N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1377 327852022020Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver NanoclustersNC2CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 10145)BiofilmDNA aptamer-enclosed silver nanoclusters (Ag-NC) were used to prevent biofilms.Potency of an aptamer-DNA enclosed Ag-NC with a decrease in the net Ld wrt control follows NC2 > NC1 > NC5 > positive control ≈ NC3 > NC6.N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1378 327852022020Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver NanoclustersNC3CCCTTTCCCTTTCCCATTCCCGTTCCCTTTCCCTTTCCCATTCCCGTTA49N/AssDNAPseudomonas Aeruginosa (ATCC 10145)BiofilmDNA aptamer-enclosed silver nanoclusters (Ag-NC) were used to prevent biofilms.Potency of an aptamer-DNA enclosed Ag-NC with a decrease in the net Ld wrt control follows NC2 > NC1 > NC5 > positive control ≈ NC3 > NC6.N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1379 327852022020Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver NanoclustersNC5ATGAGAGCGTCGGTGTGGTA-CCCTTTCCCTTTCCCATTCCCGTTCCCTTTCCCTTTCCCATTCCCGTTA-TACTTCCGCACCCTCCTACA89N/AssDNAPseudomonas Aeruginosa (ATCC 10145)BiofilmDNA aptamer-enclosed silver nanoclusters (Ag-NC) were used to prevent biofilms.Potency of an aptamer-DNA enclosed Ag-NC with a decrease in the net Ld wrt control follows NC2 > NC1 > NC5 > positive control ≈ NC3 > NC6.N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1380 327852022020Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver NanoclustersNC6ATGAGAGCGTCGGTGTGGTA20N/AssDNAPseudomonas Aeruginosa (ATCC 10145)BiofilmDNA aptamer-enclosed silver nanoclusters (Ag-NC) were used to prevent biofilms.Potency of an aptamer-DNA enclosed Ag-NC with a decrease in the net Ld wrt control follows NC2 > NC1 > NC5 > positive control ≈ NC3 > NC6.N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1381 322893692020Accurate MRSA identification through dual-functional aptamer and CRISPR-Cas12a assisted rolling circle amplificationApt AATATACACCCCACCTCGCTCCCGTGACACTAATGCTATTTTTT43N/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA) (ATCC 17802)Staphylococcus aureus Protein A (SpA)Dual-functional technology based on aptamer and CRISPR-Cas12a-assisted RCA for detection of MRSA.Fluorescence intensity shows a linear correlation with the logarithm of MRSA concentration, ranging from 10(6) to 10(2) CFU/mL, spanning six orders of magnitude.N/AN/AN/ADetectionN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1382 322893692020Accurate MRSA identification through dual-functional aptamer and CRISPR-Cas12a assisted rolling circle amplificationApt BCACCCCACCTCGCTCCCGTGACACTAATGCTATTTTTT38N/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA) (ATCC 17802)Penicillin binding protein 2a (PBP2a)Dual-functional technology based on aptamer and CRISPR-Cas12a-assisted RCA for detection of MRSA.Fluorescence intensity shows a linear correlation with the logarithm of MRSA concentration, ranging from 10(6) to 10(2) CFU/mL, spanning six orders of magnitude.N/AN/AN/ADetectionN/A3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1383 322912462020Dual aptamer assay for detection of Acinetobacter baumannii on an electromagnetically-driven microfluidic platformAptamerACAGCACCACAGACCACATATCACATGCTGTCGCCTTGCGATATCAATTCCAGTGATGTTTGTCTTCCTGCC72N/AssDNAAcinetobacter BaumanniiWhole cellDual aptamer assay to diagnose AB by using an electromagnetically-driven microfluidic system.Within 30 minutes, a limit of detection of only 100 CFU/reaction and a range of 10^2 to 10^5 CFU/reaction was obtained.N/AN/A6.8 ± 1.9 nMBiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1384 329938652020A universal signal-on electrochemical assay for rapid on-site quantitation of vibrio parahaemolyticus using aptamer modified magnetic metal-organic framework and phenylboronic acid-ferrocene co-immobilized nanolabelAptamerTTTTTTTTTCAACGAAACAGTGACTCGTTG30N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a signal-on type electrochemical aptasensor (Fe3O4@NMOF-Apt) to rapidly and on-site detect V.P.Detects V.P in the range of 10–10(9) cfu/mL with an LOD of 3 cfu/mL and within only 20 min.N/ALinearized adsorption isotherm16.8 nM (with V.P.) and 2.2 ± 0.2 nM (with Fe3O4@NMOF-Apt)BiosensorN/A5'-Amidation (NH₂)N/ANo significant difference in the measured blank and signals was observed over 3 months with aptasensor, when 91.3% of the initial signal was obtained.N/AN/AN/A
ABdb_1385 329053292020Simple Colorimetric Assay for Vibrio parahaemolyticus Detection Using Aptamer-Functionalized NanoparticlesV.P. AptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a visual colorimetric assay using aptamer-conjugated MNPs and AuNPs for the detection of V. parahaemolyticus.Shows a linear range of 10-10(6) cfu/mL, with a limit of detection of 2.4 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) or ThiolatedN/AN/AN/AN/AN/A
ABdb_1402 320378082020Gold Nanobones Enhanced Ultrasensitive Surface-Enhanced Raman Scattering Aptasensor for Detecting Escherichia coli O157:H7Apt-1AAAAAAAAAAAAAAAAAAAACCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG62N/AssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellDeveloped a one-pot step method based on capture probe (MNPs + Apt-2) and the signal probe (GNR(Apt‑1+RhB)) for SERS detection of E. coli O157:H7.Exhibited a linear range of 10-10,000 cfu/mL with a limit of detection of 3 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1403 320378082020Gold Nanobones Enhanced Ultrasensitive Surface-Enhanced Raman Scattering Aptasensor for Detecting Escherichia coli O157:H7Apt-2CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellDeveloped a one-pot step method based on capture probe (MNPs + Apt-2) and the signal probe (GNR(Apt‑1+RhB)) for SERS detection of E. coli O157:H7.Exhibited a linear range of 10-10,000 cfu/mL with a limit of detection of 3 cfu/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1410 https://doi.org/10.1007/s12161-020-01821-42020Fluorescent Turn-on Aptasensor of Staphylococcus aureus Based on the FRET Between Green Carbon Quantum Dot and Gold NanoparticleStaphylococcus aureus aptamerGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped FRET-based aptasensor with CQDs and GNPs for the detection of S. aureus.Linear detection range of 10⁸ to 10¹ CFU/mL with a detection limit (LOD) of 10 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1411 328929352020Naked-eye based point-of-care detection of E.coli O157: H7 by a signal-amplified microfluidic aptasensorAnti-E.coli O157: H7 aptamerATCCGTCACACCTGCTCTGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCGTGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O157:H7 (BNCC 191201)Whole cellDeveloped an eye-based aptasensor (EA-Sensor) for the detection of E.coli O157:H7.Display a linear range of 500–5 × 10(7) CFU/mL and LOD of 250 CFU/mL and 400 CFU/mL for buffered and milk samples.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1412 https://doi.org/10.1111/jfs.128682020Ultrasensitive detection of Listeria monocytogenes using solid-state electrochemiluminescence biosensing based on the quenching effect of ferrocene on ruthenium pyridineAptamer (ssDNA)ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria Monocytogenes (ATCC 19115, Serotype 4 b)Whole cellDeveloped an ECL biosensing switch system based on the specific recognition of an aptamer and the destruction of pyridine ruthenium by ferrocene for the detection of L. monocytogenes.Produced a good linear relationship over the concentration range of 1.4 × 10(1)–1.4 × 10(6) CFU/ml and a detection limit of 4 CFU/ml.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1413 https://doi.org/10.3390/IECB2020-070792020Detection of Listeria innocua by Acoustic AptasensorAptamerTACTATCGCGGAGACAGCGCGGGAGGCACCGGGGA35N/AssDNAListeria InnocuaWhole cellQCM-based aptasensor for the detection of pathogenic bacteria Listeria innocua.The achieved limit of detection was approximately 1.6 × 10(3) CFU/mL and broad range of 5 × 10(3)-10(6) CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1414 https://doi.org/10.1016/j.foodcont.2020.1078082020Development of a fluorescence aptasensor for rapid and sensitive detection of Listeria monocytogenes in foodL. monocytogenes AptamerGGGAGCTCAGAATAAACGCTCAATACTATCGCGGGACAGCGCGGGAGGCACCGGGGATTCGACATGAGGCCCGGATC77N/AssDNAListeria Monocytogenes (ATCC 19115)Whole cellA fluorescence aptasensor based on aptamer-UCNP and aptamer-MNP complex was developed for the detection of L. monocytogenes.A low limit of detection of 8 cfu/mL was estimated from the range of 68 to 68 × 10(6) cfu/mL.N/AN/A48.74 ± 3.11 nMBiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1436 323506132020A fluorescent aptasensor for Staphylococcus aureus based on strand displacement amplification and self-assembled DNA hexagonal structureAptamerCACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT48N/AssDNAStaphylococcus aureus (S. aureus)Whole cellA fluorescent aptasensor based on MB-apt-cDNA duplex for S. aureus in milk samples.Exhibits a broad linear range from 7 to 7 × 10(7) CFU/mL, with a detection limit of 1.7 CFU/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1437 325943192020SiC-functionalized fluorescent aptasensor for determination of Proteus mirabilisAptamerTTGCTGTAGGGGAGGAGGGTGGGT24N/AssDNAProteus Mirabilis (ATCC 12453)Whole cellDeveloped a fluorescent aptasensor based on aptamer-modified SiC quantum dots (DNA-SiC QDs) for the determination of Proteus mirabilis.The linear range is from 10(3) to 10(8) CFU/mL, and the limit of detection is 526 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1438 320706112020Rapid and sensitive detection of Salmonella Typhimurium using nickel nanowire bridge for electrochemical impedance amplificationAptamerCAGTCCAGGACAGATTCGCGAGCCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATCCACGTGGATTTCATTCAGCGATT90N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an electrochemical aptasensor using an aptamer-coated gold interdigitated microelectrode and antibody-modified NiNWs to detect Salmonella typhimurium.This electrochemical aptasensor quantitatively detected Salmonella at concentrations ranging from 10(2) to 10(6) CFU/mL within 2 h, with a detection limit of 80 CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1439 317262722020CdS quantum dots/Au nanoparticles/ZnO nanowire array for self-powered photoelectrochemical detection of Escherichia coli O157:H7E. coli aptamerATCCGTCACACCTGCTCTACTGGCCGGCTCAGCATGACTAAGAAGGAAGTTATGTGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O157:H7Surface proteinDeveloped a photoelectrochemical (PEC) platform (CdS QDs/Au NPs/ZnO NWs) for the detection of E. coli O157:H7.Exhibited a wide linear range of 10-10(7) CFU/mL with the detection limit as low as 1.125 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AAfter 15 days, the PEC aptasensor still retained 87% of its initial sensitivity.N/AN/AN/A
ABdb_1440 316550502020Rapid and sensitive detection of Salmonella with reduced graphene oxide-carbon nanotube based electrochemical aptasensorS. Typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop a biosensor using reduced graphene oxide-carbon nanotubes (rGO-CNT) nanocomposite via the hydrothermal method for label-free electrochemical detection of S. enterica.Display a wide linear dynamic range from 10(1) until 10(8) cfu/mL with a 10(1) cfu/mL of the limit of detection.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AssDNA/rGO-CNT/GCE aptasensor showed good to excellent stability when stored for 20 days in ultrapure water at 4°C.N/AN/AN/A
ABdb_1441 324711282020Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell CultureA15-1GTGGGGTTGAAAACGCCGGAGAGGGTGTGTGGGTGGGGTA40N/AssDNAMycoplasma HyorhinisMycoplasma hyorhinis-infected cellsDevelop an aptamer cocktail for detecting multiple species of mycoplasma in infected cell lines.A15-1 could bind to M. hyorhinis, and mixed mycoplasma-infected cells were detectable within 30 min.N/AFlow CytometryN/ADetectionN/A5'-Biotinylated and Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1442 324711282020Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell CultureA16-1YTGGGTGGGGTTGTCGCTAGGGGTTTAAGGGGTCGTCGTGA40N/AssDNAMycoplasma HyorhinisMycoplasma hyorhinis-infected cellsDevelop an aptamer cocktail for detecting multiple species of mycoplasma in infected cell lines.A16-1Y could bind to M. hyorhinis and mixed mycoplasma-infected cells.N/AFlow CytometryN/ADetectionN/A5'-Biotinylated and Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1443 324711282020Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture#1JATCCAGAGTGACGCAGCAGCCAACGTGCTTTCTACCTTATTTTCCGTCACTCTCACTCTGGACACGGTGGCTTAGT76N/AssDNAMycoplasma HyorhinisMycoplasma hyorhinis-infected cellsDevelop an aptamer cocktail for detecting multiple species of mycoplasma in infected cell lines.#1J could bind to unclassified mycoplasma-infected cells, but not M. hyorhinis-infected cells.N/AFlow CytometryN/ADetectionN/A5'-Biotinylated and Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1453 345676192020An aptamer-based shear horizontal surface acoustic wave biosensor with a CVD-grown single-layered graphene film for high-sensitivity detection of a label-free endotoxinAptamerCTTCTGCCCGCCTCCTTCC-TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA-GGAGACGAGATAGGCGGACACT86N/AssDNAEscherichia Coli (E. Coli) 055:B5 (L4524)Endotoxin (Lipopolysaccharide (LPS))Developed SH-SAW biosensor with chemical vapour deposition (CVD)-grown single-layered graphene (SLG) for endotoxin detection.The biosensor exhibited a linear detection range of 0-100 ng/mL and a detection limit (LOD) of 3.53 ng/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1454 332417942020Rapid and highly sensitive detection of Salmonella typhimurium in lettuce by using magnetic fluorescent nanoparticlesS. typhimurium aptamerAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Outer membrane protein (OMP)Develop a fluorescent sensor (FMNCs-Apt), based on Fe3O4 magnetic nanoparticles and aptamer-modified carbon quantum dots for the detection of S. typhimurium in lettuce.Detection limit (LOD) of 100 CFU/mL in vegetable washing solution and 138 CFU/mL in lettuce samples with a linear range of 10(3)-10(6) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1468 320959392020A fluorometric assay for rapid enrichment and determination of bacteria by using zirconium-metal organic frameworks as both capture surface and signal amplification tagAb-AptTACATGGTCAACCAAATTCTTGCAAATTCTGCATTCCTACTGT43N/AssDNAAcinetobacter BaumanniiWhole cellA fluorometric assay was developed to determine A. baumannii in blood samples by utilizing Zr-MOFs as capture probe (denoted as Zr-mMOF-p-Ab-Apt) and signal probe (denoted as F@UIO-66-NH2-p-LPS-Apt).The limit of detection of A. baumannii in blood samples is 10 cfu/mL with a linear range of 10(1)–10(5) cfu/mL.N/AN/AN/ABiosensorN/A5'-Phosphate and 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1469 320959392020A fluorometric assay for rapid enrichment and determination of bacteria by using zirconium-metal organic frameworks as both capture surface and signal amplification tagLPS-AptCTTCTGCCCGCCTCCTTCCTAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCAGGAGACGAGATAGGCGGACACT86N/AssDNAAcinetobacter BaumanniiLipopolysaccharide (LPS)A fluorometric assay was developed to determine A. baumannii in blood samples by utilizing Zr-MOFs as capture probe (denoted as Zr-mMOF-p-Ab-Apt) and signal probe (denoted as F@UIO-66-NH2-p-LPS-Apt).The limit of detection of A. baumannii in blood samples is 10 cfu/mL with a linear range of 10(1)–10(5) cfu/mL.N/AN/AN/ABiosensorN/A5'-Phosphate and 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1475 327748122020Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensorApt 2ATCAAATGTGCAGATATCAAGACGATTTGTACAAGAT37N/AssDNAEscherichia Coli (E. Coli) O157:H7 (EHEC) (NTCC 12900)Whole cellDeveloped an aptamer-based AuNPs bioassay to detect EHEC in contaminated water.Exhibited a good linear response over a wide concentration range of 876 to 107 CFU/mL and a low detection limit (LOD) of 263 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1486 321519092020Surface-enhanced Raman spectroscopic-based aptasensor for Shigella sonnei using a dual-functional metal complex-ligated gold nanoparticles dimerS. Sonnei aptamerTGAGCCCAAGCCCTGGTATGTTCTTCCCTTTTATTAGTCCTGTATTCCTCTACTGTTGCCGGCAGGTCTACTTTGGGATC80N/AssDNAShigella Sonnei (ATCC 51334)Whole cellDeveloped a SERS aptasensor using a composite material integrated with the Raman active 4-MBA ligand of the Eu-complex and citrate-stabilised Au nanoparticles (cit-Au NPs) for the detection of S. sonnei.Showed a good linear relationship in the range of 10–10(6) cfu/mL with a limit of detection (LOD) as low as 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1487 323475302020A sensitive and rapid bacterial antibiotic susceptibility test method by surface enhanced Raman spectroscopyE. coli O157:H7 aptamerGCAATGGTACGGTACTTCCCGCAGTTTGGGAAGGGTGATCGCACTATCAGAGGATTCCGTTCGGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) O157:H7 (CICC: 21530)Whole cellDeveloped a rapid antibiotic susceptibility test (AST) method and determined the MIC value by the Bacteria-aptamer@AgNPs-SERS method.When treated with 2(−3) μg/mL tigecycline for 1 h, the Raman peak intensity of E. coli O157: H7 was 882 a.u., and when the concentration of antibiotics was sub-MIC (2(−4) and 2(−5) μg/mL), the peak value of 735 cm−1 increased, and the Raman intensity was 3382 a.u. and 4213 a.u.N/AN/AN/ADetectionN/AN/AN/AN/AN/AN/AN/A
ABdb_1488 323475302020A sensitive and rapid bacterial antibiotic susceptibility test method by surface enhanced Raman spectroscopyS. aureus aptamerTCCCTACGGCGCTAACCTCCCAACCGCTCCACCCTGCCTCCGCCTCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (CICC 21600)Whole cellDeveloped a rapid antibiotic susceptibility test (AST) method and determined the MIC value by the Bacteria-aptamer@AgNPs-SERS method.When treated with 2(−1) μg/mL vancomycin for 1 h, the Raman peak intensity of S. aureus was 556 a.u., and when the concentration of antibiotics was sub-MIC (2(−2) and 2(−3) μg/mL), the peak value of 735 cm−1 increased, and the Raman intensity was 2177 a.u. and 2903 a.u.N/AN/AN/ADetectionN/AN/AN/AN/AN/AN/AN/A
ABdb_1489 https://doi.org/10.1016/j.foodcont.2019.1067612020Designing an aptamer based magnetic and upconversion nanoparticles conjugated fluorescence sensor for screening Escherichia coli in foodE.coli aptamerATCCGTCACACCTGCTCTGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCGTGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) (ATCC 8739)Whole cellA novel upconversion fluorescence sensor using magnetic nanoparticles (MNPs) and cDNA-upconversion nanoparticles (UCNPs) for E.coli was developed.Achieved a lower limit of detection (10 cfu/mL) in the linear range of 58–58 × 10(6) cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1493 328001402020Developing a dual-RCA microfluidic platform for sensitive E. coli O157:H7 whole-cell detectionsAptamerATCCGTCACACCTGCTCTATCAAATGTGCAGATATCAAGACGATTTGTACAAGATGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O157:H7Lipopolysaccharide (LPS)Developed a dual-RCA microfluidic platform for sensitive detection of E. coli O157:H7 cells.Achieved a linear range of 10(2) - 10(5) cells/mL with the limit of detection of 80 cells/mL in both orange juice and milk, thereby increasing overall detection signal intensities by approximately 250 times.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) and 3'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1496 https://doi.org/10.1016/j.sbsr.2019.1003132020Aptamer-NanoZyme mediated sensing platform for the rapid detection of Escherichia coli in fruit juiceAptamer P12–31(EC 12–31 TRUNC)CCCTCCGGGGGGGTCATCGGGATACCTGGTAAGGATA37N/AssDNAEscherichia Coli (E. Coli)Whole cellAn aptamer and NanoZyme-based colorimetric and electrochemical assay was developed for EC detection in fruit juice.Displayed superior linearity in the range of 10–10(9) CFUs/mL and a low-end detection limit of ~10 CFU within 5 min.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1497 https://doi.org/10.1016/j.foodcont.2020.1072812020A novel AuNPs colorimetric sensor for sensitively detecting viable Salmonella typhimurium based on dual aptamersApt1GAGGAAAGTCTATAGCAGAGGAGATGTGTGAACCGAGTAA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor based on AuNPs for visual detection of viable S. typhimurium.Showed a linear range from 3.3 × 10(1) to 3.3 × 10(6) CFU/mL and the detection limit of 33 CFU/mL in pure culture and 95 CFU/mL in spiked milk.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1498 https://doi.org/10.1016/j.foodcont.2020.1072812020A novel AuNPs colorimetric sensor for sensitively detecting viable Salmonella typhimurium based on dual aptamersApt2CTCCTCTGACTGTAACCACGGAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGCCGCATAGGTAGTCCAGAAGCC80N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor based on AuNPs for visual detection of viable S. typhimurium.Showed a linear range from 3.3 × 10(1) to 3.3 × 10(6) CFU/mL and the detection limit of 33 CFU/mL in pure culture and 95 CFU/mL in spiked milk.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1499 322008872020DNA aptamer-based non-faradaic impedance biosensor for detecting E. coliOMP Ag1 aptamerATCCGTCACACCTGCTCT-ACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA-TGGTGTTGGCTCCCGTAT73N/AssDNAEscherichia Coli (E. Coli) BL21 strainOuter membrane protein Ag1 (E. coli OMP Ag1)Developed an impedance-based biosensor using aptamer-functionalized Interdigitated Electrode (IDE) arrays to detect E. coli.Detection limit of 9 cfu/mL and linear concentration range of 25–1000 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1500 320000582020A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrateApt 1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a SERS aptasensor using Apt-Au-PDMS film for simultaneous multiple-pathogen detection.Can selectively detect 18 cfu/mL cells.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1501 320000582020A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrateApt 2AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a SERS aptasensor using Apt-Au-PDMS film for simultaneous multiple-pathogen detection.Can selectively detect 27 cfu/mL cells.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1502 326007572020A novel fluorometric aptasensor based on carbon nanocomposite for sensitive detection of Escherichia coli O157:H7 in milkAnti-E. coli O157:H7 aptamerCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 25922)Lipopolysaccharide (LPS)CIP@MWCNT-based fluorometric aptasensor for detection of E. coli O157:H7.The detection limit was as low as 7.15 × 10(3) cfu/mL in pure culture and 3.15 × 10(2) cfu/mL in contaminated milk with a linear range of 10(3)-10(6) cfu/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AThe fluorescent values of the CIP@MWCNT-based aptasensor were above 91.2% of the initial value at 4°C and −20°C after 4 wk.N/AN/AN/A
ABdb_1503 343474272021Aptamer-Functionalized DNA-Silver Nanocluster Nanofilm for Visual Detection and Elimination of BacteriaApt-GTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAACGGGTGGGGTGGGGTGGGG80N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDNA-templated silver nanoclusters (N4-AgNC/Apt-G) for the visual detection and effective elimination of bacteria.After treatment with 5 μM N4-AgNCs/Apt-G, a significantly reduced number of colonies was observed. At the lowest inhibitory concentration of 6.25 μM, the number of colonies decreased by only 4-fold, which is significantly less than the 7-fold reduction observed at 5 μM. Obtained recovery rates ranging from 88% to 115% of S. aureus.N/AN/AN/ADiagnostic/TherapeuticsN/AN/AN4-AgNCs/Apt-G was found to be nontoxic at low concentrations, and cell viability was more than 80% at a concentration of 6.25 μM.N/AN/AN/AN/A
ABdb_1540 346649412021Efficient Eradication of Bacterial Biofilms with Highly Specific Graphene-Based Nanocomposite SheetsS. Typhimurium aptamer (ST-NH2)TTTTTAAGCCCACTGGCGTTCGGACATCACAGCTCGTGCAGGTCGTGCCATG52N/AssDNASalmonella Typhimurium (S. Typhimurium) (CMCC 50115)Whole cellICG@GO-Apt nanosheets for eradication of biofilm associated with Salmonella Typhimurium.Shows an efficient biofilm elimination with an efficiency of greater than 99.99% in an abscess formation model.N/AN/AN/ATargeted Delivery/TherapeuticsN/A3'-Amidation (NH₂) or TAMRA, 5'-FAM Labeled or Amidation (NH₂)ICG@GO-Apt NSs had almost no cytotoxicity to HEK 293 cells, with more than 90% and lacked hemolytic activities.N/AN/AN/AN/A
ABdb_1541 332851252021An aptamer biosensor based dual signal amplification system for the detection of salmonella typhimuriumS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ASI 1174)Whole cellDeveloped a signal-cascade-amplification method named “immuno-HCR-SERS” for the detection of S. typhimurium.Highly sensitive detection showing a linear range from 10 to 10(5) CFU/mL, with a limit of detection (LOD) of 6 CFU/mL in 3.5 h.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1542 348288202021A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of Salmonella typhimurium in MilkCapture aptamer (Bapt)GAGGAAAGTCTATAGCAGAGGAGATGTGTGAACCGAGTAA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colourimetric assay based on aptamer-catalysed hairpin assembly signal amplification strategy (Y-CHA ) for the detection of S. typhimurium in milk.Exhibited a concentration range of 10(2) to 10(6) CFU/mL, with a sensitivity of 2.4 × 10(2) CFU/mL under optimal conditions and 2.8 × 10(3) CFU/mL in real milk samples.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1543 348288202021A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of Salmonella typhimurium in MilkTarget aptamer (Tapt)TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colourimetric assay based on aptamer-catalysed hairpin assembly signal amplification strategy (Y-CHA ) for the detection of S. typhimurium in milk.Exhibited a concentration range of 10(2) to 10(6) CFU/mL, with a sensitivity of 2.4 × 10(2) CFU/mL under optimal conditions and 2.8 × 10(3) CFU/mL in real milk samples.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1544 340516012021Sensitive detection of S. Aureus using aptamer- and vancomycin -copper nanoclusters as dual recognition strategyAptamerGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped an aptamer- and antibiotic-based dual detection sensor, combines copper nanoclusters (CuNCs) for the detection of S. aureus.Showed a linear range of 10(2)-10(8) CFU/mL, and a detection limit of 80 CFU/mL after 45 min.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1545 https://doi.org/10.1016/j.electacta.2021.1396332021A new electrochemical aptasensor based on gold/nitrogen-doped carbon nano-onions for the detection of Staphylococcus aureusAnti-S. aureus aptamerTCGGCACGTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC43N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an electrochemical aptasensor based on SPEs modified with AuNPs and NCNOs for the detection of S. aureus.Measured S. aureus quickly with a limit of detection of 3 CFU/mL in the linear range of 10–10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1546 346609932021Detection of Listeria monocytogenes Using Luminol-Functionalized AuNF-Labeled Aptamer Recognition and Magnetic SeparationAptamerATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria Monocytogenes (ATCC 19115)Whole cellDeveloped an aptamer-linked biofunctional magnetic nanomaterial system for highly sensitive and specific LM detection.Display a linear concentration range 1.0 × 10(1)-1.0 × 10(5) CFU/mL and detect L. monocytogenes at levels as low as 6 CFU/mL in milk samples.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1547 341072102021A Low-Field Magnetic Resonance Imaging Aptasensor for the Rapid and Visual Sensing of Pseudomonas aeruginosa in Food, Juice, and WaterAnti-P. aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellDeveloped a low-field magnetic resonance imaging (LF-MRI) aptasensor based on the difference in magnetic behaviour of two magnetic nanoparticles with diameters of 10 (MN10) and 400 nm (MN400) for the rapid detection of P. aeruginosa.Show a detection limit of 100 cfu/mL with a wide linear range from 3.1 × 10(2) to 3.1 × 10(7) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1548 343960092021DNA Aptamer-Conjugated Magnetic Graphene Oxide for Pathogenic Bacteria Aggregation: Selective and Enhanced Photothermal Therapy for Effective and Rapid KillingMRSA aptamerATCCAGACGTGACGCAGC-ATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTG-TGGACACGGTGGCTTAGTA755'-ATCCAGACGTGACGCAGC-N38-TGGACACGGTGGCTTAGTA-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellApt@MGO nanoplatform for selective and rapid eradication of MRSA under NIR laser irradiation.Apt@MGO resulted in ∼78% MRSA and over >97% MRSA cell inactivation in dispersed and aggregated states, respectively, under 200 seconds of exposure to NIR irradiation (808 nm, 1.1 W cm–2).N/AN/AN/ATargeted Delivery/TherapeuticsN/A3'-Amidation (NH₂) and 5'-FITC LabeledThe aptamer itself did not cause any cell activation when incubated with MRSA cells, and also under no NIR laser illumination.N/AN/AN/AN/A
ABdb_1549 330760892021Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimuriumAptamer1AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor using gold nanoclusters (aptamers@BSA-AuNCs) for the detection of S. typhimurium.Exhibited a wide linear range of 10(1)-10(6) cfu/mL with a detection limit as low as 1 cfu/mL.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1550 330760892021Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimuriumAptamer2TATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric aptasensor using gold nanoclusters (aptamers@BSA-AuNCs) for the detection of S. typhimurium.Exhibited a wide linear range of 10(1)-10(6) cfu/mL with a detection limit as low as 1 cfu/mL.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1551 330761472021Ratiometric fluorescence resonance energy transfer aptasensor for highly sensitive and selective detection of Acinetobacter baumannii bacteria in urine sample using carbon dots as optical nanoprobesAb aptamerCAGCACCACAGACCACATATCACATGCTGTCGCCTTGCGATATCAATTCCAGTGATGTTTGTCTTCCTGCC71N/AssDNAAcinetobacter BaumanniiWhole cellDevelop an ingenious ratiometric fluorescent aptasensor for the detection of (Ab) bacteria based on FRET between ortho-phenylenediamine carbon dots (o-CD), nitrogen-doped carbon nanodots (NCND), and graphene oxide (GO).Display linear range from 2.0 × 10(3) to 4.5 × 10(7) cfu/mL and the low detection limit of 3.0 × 10(2) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AAfter a storage period of 4 weeks, over 90% of the initial response remained of the aptasenosr.N/AN/AN/A
ABdb_1552 https://doi.org/10.1016/j.microc.2021.1063882021Rapid and sensitive determination of Pseudomonas aeruginosa by using a glassy carbon electrode modified with gold nanoparticles and aptamer-imprinted polydopamineAptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellDevelop an electrochemical sensor based on a combination of aptasensing and molecular imprinting employing GCE/AuNP/Aptamer-MIP for ultrasensitive detection of P. aeruginosa.Exhibited a wide linear dynamic concentration range of 10(1) to 10(7) CFU/ml with a low detection limit of 1 CFU/ml.N/AN/AN/ADetectionN/A5'-Amidation (NH₂)N/AThe GCE/AuNP/Aptamer-MIP aptasensor signal remained unchanged, and only a 3% decrease in the peak current occurred after 50 repetitive cycles.N/AN/AN/A
ABdb_1553 345787622021Aptasensor for the Detection of Mycobacterium tuberculosis in Sputum Utilising CFP10-ESAT6 Protein as a Selective BiomarkerAptamerGCCTGTTGTGAGCCTCCTAACCCCATCTTATACGTATATGGACTCATCTCGACCCCCGATAGGCTTGGTACATGCTTATTCTTGTCTCCC90N/AssDNAMycobacterium Tuberculosis (M.Tb.)ESAT6/CFP10 fusion proteins (EC proteins)Developed a portable electrochemical aptamer-antibody-based sandwich biosensor via CapApt/GP/PANI-modified SPGE surface for M. tb. detection in clinical sputum samples.Detection of the CFP10-ESAT6 antigen ranged from 5 to 500 ng/mL, with a detection limit of 1.5 ng/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1554 349402682021One-Step Fabrication of Stimuli-Responsive Chitosan-Platinum Brushes for Listeria monocytogenes DetectionAptamerATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria Monocytogenes (ATCC 15313)Internalin A (InlA)Developed a label-free electrochemical biosensor using a new one-step simultaneous sonoelectrodeposition of platinum and chitosan (CHI/Pt) to create a biomimetic nanostructure for Listeria monocytogenes detection.LOD of the CHI/Pt-aptamer brushes in PBS was 2.5 ± 0.3 CFU/mL, 3.3 ± 0.9 CFU/mL in chicken broth.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1555 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorE18R-42CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) (CICC 10899)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1556 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorE17F-37ATCAAATGTGCAGATATCAAGACGATTTGTACAAGAT37N/AssDNAEscherichia Coli (E. Coli) (CICC 10899)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1557 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA1TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG40N/AssDNAVibrio Parahaemolyticus (CICC 21617)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1558 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA3TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (CICC 21617)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1559 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA18PATAGGAGTCACGACGACCAGAAGCGGGCAGCTCCACCGGTAGGCTCCGAGTCATCACGGTCGTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (CICC 21617)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1560 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorB1PATAGGAGTCACGACGACCAGGGGGGCGGGCGGGCCAGGCGAGGGGCAGGGCGTGGAGGGCTATGTGCGTCTACCTCTTGA80N/AssDNACronobacter Sakazakii (CICC 21562)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/A56.56 ± 10.51 nMBiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1561 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorB16PATAGGAGTCACGACGACCAGGGATGGGGCCGCGCGGGGTGGGGGCGCGAGGGCCGGCGGGTATGTGCGTCTACCTCTTGA80N/AssDNACronobacter Sakazakii (CICC 21562)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/A49.83 ± 7.69 nMBiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1562 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorB25PATAGGAGTCACGACGACCAGGCCGCCCGGGGACGGGGGCGGCGGGGAGGAGGGCGGCGGGTATGTGCGTCTACCTCTTGA80N/AssDNACronobacter Sakazakii (CICC 21562)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Detection limit as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1563 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA6GGGCAGGTGTTATGTGTACTGCTACAGTGTGGTTG35N/AssDNASalmonella Typhimurium (S. Typhimurium) (CICC 21484)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Show a linear range for monoaptasensor in the range of 1 × 10(1) to 1 × 10(9) CFU/mL and for polyaptasensor ranging from 1 × 10(1) to 1 × 10(7) CFU/mL with an LOD of 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1564 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA16GGCAGGACAACAGCGTGTAGTATCAGCTTACGGTG35N/AssDNASalmonella Typhimurium (S. Typhimurium) (CICC 21484)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Show a linear range for monoaptasensor in the range of 1 × 10(1) to 1 × 10(9) CFU/mL and for polyaptasensor ranging from 1 × 10(1) to 1 × 10(7) CFU/mL with an LOD of 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1565 345534392021The systemic characterization of aptamer cocktail for bacterial detection studied by graphene oxide-based fluorescence resonance energy transfer aptasensorA18PCCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG35N/AssDNASalmonella Typhimurium (S. Typhimurium) (CICC 21484)Whole cellDeveloped a fluorescence resonance energy transfer polyaptasensor based on an aptamer cocktail/graphene oxide for bacterial detection.Show a linear range for monoaptasensor in the range of 1 × 10(1) to 1 × 10(9) CFU/mL and for polyaptasensor ranging from 1 × 10(1) to 1 × 10(7) CFU/mL with an LOD of 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1566 347313142021An electrochemical aptasensor for Mycobacterium tuberculosis ESAT-6 antigen detection using bimetallic organic frameworkEBAGGGAGCTCAGAATAAACGCTCAACCTCCCTGGGTCACCATAGACTCCATCTAAGATGCTTCGACATGAGGCCCGGATC78N/AssDNAMycobacterium Tuberculosis (M.Tb.)ESAT-6 (6-kDa early secretory antigenic target)A label-free electrochemical aptasensor was developed using NG@Zr-MOF-on-Ce-MOF@Tb nanohybrid for sensitive detection of ESAT-6.Displayed a wide linear range from 100 fg/mL to 10 ng/mL with a limit of detection (LOD) of 12 fg/mL.N/AN/AN/ABiosensorN/A5'-Phosphate (PO4(-3))N/AThe current remained at 95.77% and 88.72% of the initial current after storing at at 4℃ for 8 days and 16 days, respectively.N/AN/AN/A
ABdb_1567 337808522021Microfluidic thread-based electrochemical aptasensor for rapid detection of Vibrio parahaemolyticusAptamerATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a thread-based microfluidic nano-aptasensor based on MoS2 modified threaded electrodes for V. parahaemolyticus detection.The proposed aptasensor has a dynamic detection range of 10–10(6) CFU/mL, a detection limit of 5.74 CFU/mL, and an assay time of 30 min.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1568 334217622021Controllable design of a nano-bio aptasensing interface based on tetrahedral framework nucleic acids in an integrated microfluidic platformAptamerATCCGTCACACCTGCTCTATCAAATGTGCAGATATCAAGACGATTTGTACAAGATGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O157: H7 (ATCC 43889)Whole cellDeveloped a tetrahedral framework nucleic acids-based aptasensing interface in the microfluidic system for E. coli O157: H7 detection and antimicrobial susceptibility testing (AST) determination.A calibration curve was constructed over 10(1) to 10(5) CFU/mL, with a limit of detection of 10(1) CFU/mL.N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-CGGAATTCCG)N/AN/AN/AN/AN/A
ABdb_1569 334797972021A turn-on-type fluorescence resonance energy transfer aptasensor for vibrio detection using aptamer-modified polyhedral oligomeric silsesquioxane-perovskite quantum dots/Ti3C2 MXenes composite probesVP aptamerTTTTTTTTTCAACGAAACAGTGACTCGTTG30N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a turn-on–type aptasensor based on FRET pair using aptamer modified polyhedral oligomeric silsesquioxane-perovskite quantum dots (POSS-PQDs-Apt) as signal probe and titanium carbide (Ti3C2) MXenes as quencher for Vibrio parahaemolyticus (VP) determination.Under optimized conditions, the assay can determine VP in the concentration range 10(2) - 10(6) cfu/mL with the detection limit (LOD) of 30 cfu/mL and 100 cfu/mL by naked eye in aquaculture water.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1578 338364312021Ultra-sensitive photoelectrochemical aptamer biosensor for detecting E. coli O157:H7 based on nonmetallic plasmonic two-dimensional hydrated defective tungsten oxide nanosheets coupling with nitrogen-doped graphene quantum dots (dWO3•H2O@N-GQDs)E.coli O157:H7 aptamerGCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDeveloped a novel PEC aptasensor based on dWO3•H2O@N-GQDs for ultrasensitive detection of E. coli O157:H7.Achieved a limit of detection of 0.05 CFU/mL and a wide linear detection range from 0.1 to 10(4) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1579 https://doi.org/10.1016/j.snb.2021.1303372021Electrochemical aptasensor for simultaneous detection of foodborne pathogens based on a double stirring bars-assisted signal amplification strategyApt-V.PTGGCTAGCTCAGTCATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTCCGCG60N/AssDNAVibrio ParahaemolyticusWhole cellDeveloped a double stirring bar-based signal-amplified strategy using aptamer-embedded tetrahedral DNA nanostructures for the simultaneous electrochemical detection of Vibrio parahaemolyticus (V.P) and Salmonella typhimurium (S.T).Display a lower detection limit of 4 CFU/mL for V.P. within a detection range of 10–10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1580 https://doi.org/10.1016/j.snb.2021.1303372021Electrochemical aptasensor for simultaneous detection of foodborne pathogens based on a double stirring bars-assisted signal amplification strategyApt-S.TTGGCTAGCTCAGTCATATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAGCCGCG60N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped a double stirring bar-based signal-amplified strategy using aptamer-embedded tetrahedral DNA nanostructures for the simultaneous electrochemical detection of Vibrio parahaemolyticus (V.P) and Salmonella typhimurium (S.T).Display a lower detection limit of 7 CFU/mL for S.T. within a detection range of 10–10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1581 333031522021Hollow carbon nanocapsules-based nitrogen-doped carbon nanofibers with rosary-like structure as a high surface substrate for impedimetric detection of Pseudomonas aeruginosaAptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellDeveloped an electrochemical aptasensor based on hollow carbon nanocapsule-based nitrogen-doped carbon nanofibers (CNCNF) with a rosary-like structure for the detection of PA.The linear range and LOD of the aptasensor were 101-107 CFU/ml and 1 CFU/ml.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AAfter 8 days of storage in Fe(CN)6 3-/4- at 4°C, the electrode was found to be 89% of its initial activity.N/AN/AN/A
ABdb_1582 https://doi.org/10.1016/j.microc.2021.1061322021A novel photoelectrochemical aptamer sensor based on rare-earth doped Bi2WO6 and Ag2S for the rapid detection of Vibrio parahaemolyticusAptamerTCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a novel photoelectrochemical aptasensor based on rare-earth doped Bi2WO6 and Ag2S for the detection of V. parahaemolyticus.Showed a wide linear range from 3.2 × 10(2) CFU/mL to 3.2 × 10(8) CFU/mL, and a detection limit of 40 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AAfter 2 weeks of storage at 4°C in a refrigerator, the biosensor retained 95% of its initial response.N/AN/AN/A
ABdb_1583 349454472021A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes DetectionAptamerATACCAGCTTATTCAATTCCAAAAGCGCACCCATATATGTTCTATGTCCCCCACCTCGAGATTGCACTTACTATCT76N/AssDNAListeria Monocytogenes (ATCC 19115)Whole cellDeveloped a photoelectrochemical aptasensor based on WO3, CdTe QDs, and Exo I auxiliary signal amplification for the detection of L. monocytogenes.Showed a detection limit of 45 CFU/mL over the concentration range of 1.3 × 10(1) - 1.3 × 10 (7) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1584 340537672021A novel smartphone-based colorimetric aptasensor for on-site detection of Escherichia coli O157:H7 in milkAptamer (Apt)CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellA colorimetric aptasensor was developed using GNP-Apt coupling with an MWCNT@CIP probe for detecting E. coli O157:H7 in milk.Display a concentration of 8.43 × 10(3) cfu/mL in pure culture, and 5.24 × 10(2) cfu/mL in artificially contaminated milk after 1h.N/AN/AN/ABiosensorN/A5'-Thiolated (SH-C6)N/AN/AN/AN/AN/A
ABdb_1585 349707242021Colorimetric determination of Listeria monocytogenes using aptamer and urease dual-labeled magnetic nanoparticles and cucurbit[7]uril-mediated supramolecular assembly of gold nanoparticleAptamerTTTTTTTTTTATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT57N/AssDNAListeria Monocytogenes (ATCC 19111)Whole cellDeveloped a colorimetric strategy using apt-MNP-urease conjugate for the detection of L. monocytogenes.Achieved a concentration range from 10 to 10(6) cfu/mL, and the visual determination can be done down to 10 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1586 340744322021One-step colorimetric detection of Staphylococcus aureus based on target-induced shielding against the peroxidase mimicking activity of aptamer-functionalized gold-coated iron oxide nanocompositesAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped a colorimetric sensor using apt-Fe3O4/Au nanocomposites for the detection of Staphylococcus aureus.Display a linear range from 10 to 10(6) CFU/mL, with the visible limit of detection as low as 10 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1587 335903782021Sensitive colorimetric aptasensor based on g-C3N4@Cu2O composites for detection of Salmonella typhimurium in food and waterAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA colorimetric aptasensor using graphitic carbon nitride (g-C3N4) nanosheets and copper oxide(I) (Cu2O) nanocrystals was developed for detecting S. typhimurium.Exhibited linear range from 1.5 × 10(1) to 1.5 × 10(5) CFU/ml, with a detection limit of 15 CFU/ml.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1588 347535772021An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tagsS. aureus-aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an aptasensor using WGA modified-Fe3O4@Au MNPs and Streptavidin (SA)/aptamer co-functionalized SERS tags for S. aureus and L. mono detection.The LOD for S. aureus was 3 cells/mL and a favorable linear relation (10-10(7) cells/mL).N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_1589 347535772021An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tagsL. mono-aptamerATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria MonocytogenesWhole cellDeveloped an aptasensor using WGA modified-Fe3O4@Au MNPs and Streptavidin (SA)/aptamer co-functionalized SERS tags for S. aureus and L. mono detection.The LOD for L. mono was 5 cells/mL and a favorable linear relation (10-10(7) cells/mL).N/AN/AN/ABiosensorN/A5'-Biotinylated (biotin-C6)N/AN/AN/AN/AN/A
ABdb_1590 338949562021A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognitionE. coli-aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 35150)Staphylococcus aureus Protein A (SpA)Developed a SERS aptasensor using aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) and PA modified-SERS tags (Au@DTNB@PA) for the detection of bacteria.The limit of detection (LOD) was 10 cells/mL for E. coli.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1591 338949562021A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognitionL. mono-aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNAListeria MonocytogenesStaphylococcus aureus Protein A (SpA)Developed a SERS aptasensor using aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) and PA modified-SERS tags (Au@DTNB@PA) for the detection of bacteria.The limit of detection (LOD) was 10 cells/mL for L. mono.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1592 338949562021A universal SERS-label immunoassay for pathogen bacteria detection based on Fe3O4@Au-aptamer separation and antibody-protein A orientation recognitionS. typhi-aptamerATAGGAGTCACGACGACCAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGCGTCTACCTCTTGACTAAT87N/AssDNASalmonella Typhimurium (S. Typhimurium)Staphylococcus aureus Protein A (SpA)Developed a SERS aptasensor using aptamer-conjugated Fe3O4@Au magnetic nanoparticles (MNPs) and PA modified-SERS tags (Au@DTNB@PA) for the detection of bacteria.The limit of detection (LOD) for S. typhi was 25 cells/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1593 340415802021Fabrication of gold/silver nanodimer SERS probes for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an aptamer-facilitated gold/silver nanodimer SERS probe for the simultaneous detection of the two bacteria with the help of magnetic separation enrichment.Achieved a linear range of 10(2) to 10(7) cfu/mL, with a detection limit of 50 cfu/mL.N/AN/AN/ABiosensorN/A5′-SH-A-Cy3 and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1594 340415802021Fabrication of gold/silver nanodimer SERS probes for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureusS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an aptamer-facilitated gold/silver nanodimer SERS probe for the simultaneous detection of the two bacteria with the help of magnetic separation enrichment.Achieved a linear range of 3.2 × 10(2) to 3.2 × 10(7) cfu/mL, with a detection limit of 96 cfu/mL.N/AN/AN/ABiosensorN/A5′-SH-A-Rox and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1595 348692162021Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7AptamerCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellA carbon dots-Fe3O4 nanomaterial (CDs-Fe3O4)-based electrochemical aptasensor for E. coli O157:H7 detection was developed.Exhibited the detection range of 10–10(8) CFU/ml, and the detection limit of 6.88 CFU/ml.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1596 344067472021Upconversion Nanoprobes Based on a Horseradish Peroxidase-Regulated Dual-Mode Strategy for the Ultrasensitive Detection of Staphylococcus aureus in MeatS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a colorimetric and fluorescent dual-mode nanoprobe using apt-MNPs and HRP-UCNPs-cDNA for the detection of S. aureus.Obtained limits of detection of 22 CFU/mL for fluorescence and 20 CFU/mL for colorimetry in a linear range of 56–5.6 × 10(6) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1597 https://doi.org/10.1007/s13738-021-02384-92021Rapid and sensitive detection of Escherichia coli O157:H7 based on silver nanocluster fluorescent probeE. coli O157:H7 aptamerTGAGCCCAAGCCCTGGTATGCGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTTGGCAGGTCTACTTTGGGATC80N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDetection of E. coli O157:H7 in food based on aptamer-modified silver nanocluster fluorescent probes.The linear range was 10–10(6) CFU/mL, and the detection limit was 0.2549 CFU/mL in the buffer and 0.6031 CFU/mL in the milk simulation sample.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1598 10.33263/BRIAC112.870287152021Novel Competitive Voltammetric Aptasensor Based on Electrospun Carbon Nanofibers-Gold Nanoparticles Modified Graphite Electrode for Salmonella enterica serovar DetectionAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an electrochemical aptasensor based on aptamer-linked GNPs/CNF-Chi/GEs for the detection of Salmonella enterica Serovar.Exhibited a linear range of 10 to 10(5) CFU/mL with the limit of detection (LOD) 1.223 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1611 355737942022Aptamer-Targeted Drug Delivery for Staphylococcus aureus BiofilmSA31GCAATGGTACGGTACTTCC-TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (DSM 20231)Whole cellAptamer-targeted liposomes encapsulating antibiotics for accumulation and delivery to eradicate S. aureus biofilm.SA31-modified liposomes fully eradicated all viable, culturable bacteria in all biofilm samples.N/AN/AN/ATargeted Delivery/TherapeuticsN/A3'-5-propargylamino-ddUTP-Cy5 or 3'-N3 modifiedN/ASA23 appeared more stable than the other aptamers in plasma over a 16-hour incubation period.N/AN/AN/A
ABdb_1614 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateAptBHAAACCTGCTTATCTAAGGGGGGGAGGGGGGGTAGTGGGTGGGT43N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.AptBH-AgNPs at a concentration of 100 μg/mL after 48 h inhibited 43% of the biofilm formation and degraded 63% of the formed biofilm.N/AN/AN/ATherapeuticsN/A3'-BiotinylatedAgNPs-AptBH is weakly toxic, with cell viability of 96% even at 100 μg/mL. At 75 and 100 μg/mL, these concentrations caused ∼approximately 9% and 11% hemolysis, respectively.AgNPs-AptBH complex has not decomposed over time for 360 min in the cell culture medium.Best CandidateN/AN/A
ABdb_1615 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G10-11CAACCTGCTTATCTAAGGGGGGGAGGGGGGGTTGTGGGTAGGT43N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1616 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G11-6ATACTATCGCATTCCTTCCGAGGGGGGAGGGGGGGGTGGGGGTCGGT47N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1617 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G11-5ATACATCTTAAGTCTCGTGGGGGGAGGGGGGGTTGGTGGGCTTT44N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1618 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G11-20CTACGTCTAGATTCCAGTCGAGGGGGGAGGGGGGGTTTTGGATCGGT47N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1619 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateSMa#G10-6ACACCAGCGTATTCTCTTGGGGGGAGGGGGGGTTGGGGGTCGGT44N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1620 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-1GCATCGGTCCTGAAGTTGCTCTAGTGCCCGTGTGCTCAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1621 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-2GGGCTAGCCCCGGATCACCACTTTCCCTGCTTGATGCAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1622 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-3TGTAACGGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1623 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-4TGGCACCGTGGAGTCGTGTTGAGGAGGCGCAATGCGTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1624 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-5CCTAACGTTCTCTCCTCGCTCCTCAAGGAGCCACGCTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1625 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-6CGGTTTTCGCGCTATTTCCGTACAACCCGCGACGCCTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1626 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateASM-7GTGTTTCGCGCTATTTCCGTACAACCCGCGGACGCCTAA39N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1627 362768992022Investigating the anti-streptococcal biofilm effect of ssDNA aptamer-silver nanoparticles complex on a titanium-based substrateFAMGCAATGGTACGGTACTTCCCAAAAGTGCACGCTACTTTGCTAA43N/AssDNAStreptococcus Mutans (PTCC 1683)Fibronectin/fibrinogen-binding protein (FBP)Silver nanoparticles-aptamer complex (AptBH-AgNPs) that binds to the surface receptors of streptococcal strains and inhibits biofilm formation.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1628 358424602022DNA-aptamer-nanographene oxide as a targeted bio-theragnostic system in antimicrobial photodynamic therapy against Porphyromonas gingivalisAptamerTATGCCAGCATTTCGCCAACGGTGGTCATACAGTGTGAA39N/AssDNAPorphyromonas GingivalisWhole cellDNA-aptamer-NGO, a targeted bio-theragnostic system against P. gingivalis for antimicrobial photodynamic therapy (aPDT).MIC of DNA-aptamer-NGO was 62.5 nM, and MBC was 125 nM. aPDT using 1/2 × and 1/4 × MBC of DNA-aptamer-NGO plus irradiation of the diode laser light (1 min) has a significantly anti-biofilm effect; percentage of apoptosis cells treated with DNA-aptamer-NGO at 1/2 × and 1/4 × MIC plus diode laser were 13.9% and 12.7%; and 1/2 × MIC of DNA-aptamer-NGO, resulted in 40.3% inactivation of metabolic activity compared to 1/4 × MBC of DNA-aptamer-NGO, where only 22.2% inactivation.N/AN/AN/ADiagnostic/TherapeuticsN/AFAM LabeledAt high concentrations of DNA-aptamer-NGO, incubated for 24 hours with human red blood cells, the percentage did not exceed 5%. Also, at different concentrations, the mean percentage of HGF cell viability ranges from 95.2% to 87.6%.N/AN/AN/AN/A
ABdb_1639 https:doi.org10.1016j.arabjc.2022.1042742022Application of G-quadruplex aptamer conjugated MSNs to deliver ampicillin for suppressing S. aureus biofilm on mice bonePA763’-ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCTACAATCGTAATCAGTTAG-5’76N/AssDNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)MSNs-APT-AMP nanosystem for antibiofilm activity against S. aureus biofilm.N/AN/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1640 https:doi.org10.1016j.arabjc.2022.1042742022Application of G-quadruplex aptamer conjugated MSNs to deliver ampicillin for suppressing S. aureus biofilm on mice bonePA633’-ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCTACAAT-5’63N/AssDNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)MSNs-APT-AMP nanosystem for antibiofilm activity against S. aureus biofilm.No significant biofilm on the surface of the bone after 48 h treatment with 100 µg/mL of the three-component system.N/AN/AN/ATherapeuticsN/AN/ANo significant toxicity at 100 µg/mL for MCF-7 cells in 48 h.N/ABest CandidateN/AN/A
ABdb_1641 https:doi.org10.1016j.snb.2022.1327522022Novel nanozyme-catalyzed and magnetically assisted colorimetric biosensor for Staphylococcus aureus detection with a low matrix effect from complex environmentsSA31GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Staphylococcus aureus Protein A (SpA)Vancomycin-modified magnetic nanoparticles (Fe3O4 @Au-Van MNPs) combined with an aptamer were prepared for S. aureus detection.The biosensor can distinguish more than 100 CFU/mL of pathogens by the naked eye and LOD of 2 CFU/mL for S. aureus.N/AN/AN/ABiosensorN/AFAM LabeledN/AN/AN/AN/AN/A
ABdb_1648 359343732022A surface-enhanced Raman scattering aptasensor for Escherichia coli detection based on high-performance 3D substrate and hot spot effectE. coli AptCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCG45N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped SERS aptasensor composed of Apt-AgNPs-CS gel and GNS@4-MBA-Apt NPs for detection of E. coli in food.Display a detection limit of 3.46 CFU/mL with a wide dynamic linear range from 3.2 × 10(1) to 3.2 × 10(7) CFU/mL and a good recovery rate (>90%).N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1649 https://doi.org/10.1021/acsanm.2c015482022Zirconium-Based Metal–Organic Framework and Ti3C2Tx Nanosheet-Based Faraday Cage-Type Electrochemical Aptasensor for Escherichia coli DetectionAptamerCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7Lipopolysaccharide (LPS)A Faraday-cage-type electrochemical aptasensor based on Zr-Fc MOF/AuNPs/4-MPBA framework was developed for the detection of E. coli O157:H7.Display a detection limit of 3 CFU/mL and a linear range of 10-1.0 × 10(5) CFU/mL.N/AN/AN/ABiosensorN/A5'-PhosphateN/A95.6% of the initial current of the aptasensor was reserved when stored for 14 days at 4°C.N/AN/AN/A
ABdb_1650 358691072022An innovative dual recognition aptasensor for specific detection of Staphylococcus aureus based on Au/Fe3O4 binary hybridAptGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 6538)Whole cellDeveloped an imprinted aptasensor (apt-AuNPs@ Fe3O4) for the quantification of S. aureus.Exhibited a wide linear range of 10(1)-10(7) CFU/mL with a Limit of Detection (LOD ) of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AThe aptasensor retained approximately 95% of its original activity for four weeks.N/AN/AN/A
ABdb_1651 358297782022An ultrasensitive sandwich-type electrochemical aptasensor using silver nanoparticle/titanium carbide nanocomposites for the determination of Staphylococcus aureus in milkAptsGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CICC 23656)Whole cellDeveloped a novel sandwich-type electrochemical aptasensor using AgNPs@Ti3C2 nanocomposites for the detection of S. aureus.Showed a low detection limit (LOD) of 1 CFU/mL and a linear range of 52–5.2 × 10(7) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/A92.68% of the initial current of the aptasensor was reserved when stored for 15 days at 4°C.N/AN/AN/A
ABdb_1652 358702832022An ultrasensitive and dual-recognition SERS biosensor based on Fe3O4@Au-Teicoplanin and aptamer functionalized Au@Ag nanoparticles for detection of Staphylococcus aureusS. aureus AptGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellThe SERS biosensor based on Fe3O4@Au-Tcp NPs as a capture probe and Au@Ag-DTNB-Apt NPs as a signal probe for the detection of S. aureus.Showed detection limit of 1.09 CFU/mL with a broad dynamic linear range of 7.6 × 10(1)-7.6 × 10(7) CFU/mL within 50 min.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1653 352003472022Paper-Based Electrodes Conjugated with Tungsten Disulfide Nanostructure and Aptamer for Impedimetric Detection of Listeria monocytogenesAptamerATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria MonocytogenesWhole cellDeveloped an aptasensor based on ePAD that employs a tungsten disulfide (WS2)/aptamer hybrid for the detection of L. monocytogenes.The limit of detection (LoD) was 10 CFU/mL, with a linear range of 10(1)-10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AAptasensor gives a stable impedance response over a period of 15 days.N/AN/AN/A
ABdb_1654 359343422022Sandwich fluorometric method for dual-role recognition of Listeria monocytogenes based on antibiotic-affinity strategy and fluorescence quenching effectAptamerTTTTTTTTTTATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT57N/AssDNAListeria Monocytogenes (ATCC 19111)Whole cellDeveloped a sandwich fluorimetric method (MNPs-Van) for dual-role recognition of L. monocytogenes.Achieved a low detection limit (LOD) of 2.8 × 10(2) CFU/mL in 1.5 h with a concentration range over 10(2)-2 × 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1655 https://doi.org/10.1016/j.snb.2022.1316542022Dual recognition and highly sensitive detection of Listeria monocytogenes in food by fluorescence enhancement effect based on Fe3O4@ZIF-8-aptamerL. monocytogenes aptamerTACTATCGCGGAGACAGCGCGGGAGGCACCGGGGA35N/AssDNAListeria Monocytogenes (ATCC 15313)Whole cellDeveloped a fluorescence enhancement effect-based detection strategy based on Fe3O4@ZIF-8@aptamer for L. monocytogenes.The linear range for detection of pure culture was 1.4 × 10(1) to 1.4 × 10(7) CFU/mL, with a detection limit of 0.88 CFU/mL in about 70 min.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1656 364185442022Colorimetric detection of Pseudomonas aeruginosa by aptamer-functionalized gold nanoparticlesAptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellDeveloped a colorimetric biosensor using aptamer-functionalized AuNPs for identifying P. aeruginosa.P. aeruginosa was detected after 5 h for concentrations from 10(8) to 10(5) CFU/mL, with 10(5) and 10(4) CFU/mL being the detection limits for colour change by the naked eye and UV-Vis spectrometry, respectively.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1657 358842432022Label-Free Electrochemical Aptasensor for the Detection of the 3-O-C12-HSL Quorum-Sensing Molecule in Pseudomonas aeruginosaAPTGCAATGGTACGGTACTTCCCGGGGCCCGCTTCTGGTGCGGTGTACTAGTGACCGCAAAAGTGCACGCTACTTTGCTAA78N/AssDNAPseudomonas Aeruginosa (ATCC 27853)N-3-oxo-dodecanoyl L-homoserine lactone (3-O-C12-HSL)An electrochemical aptasensor using a carbon-based SPE modified with AuNPs was developed for the detection of 3-O-C12-HSL.Exhibited a logarithmic range from 0.5 to 30 µM, with a limit of detection of 145 ng mL−1 (0.5 µM).N/ASurface Plasmon Resonance (SPR)106.7 ± 9.3 nMBiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1658 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K3CCGGAATTCCTAATACGACTCCCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTGTATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellDetermine the antibiofilm activity and binding specificity of the polyclonal DNA aptamers on S. aureus BPA-12 and E. coli EPEC 4.Showed the percentage of antibiofilm activity against S. aureus BPA-12 (25.8%) and E. coli EPEC 4 (0.3%).N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1659 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K4CCGGAATTCCTAATACGACTCCCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCCTATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the percentage of antibiofilm activity against S. aureus BPA-12 (26.3%) and E. coli EPEC 4 (2.8%).N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1660 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K6CCGGAATTCCTAATACGACTCGCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGATATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the highest percentage of antibiofilm activity against S. aureus BPA-12 (37.4%).N/AN/AN/ATherapeuticsN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_1661 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K13CCGGAATTCCTAATACGACTCCACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGGTATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the percentage of antibiofilm activity against S. aureus BPA-12 (31.8%) and E. coli EPEC 4 (9.4%).N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1662 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K15CCGGAATTCCTAATACGACTCCAGGACAGTACTCTGGACGGCAATACGTATATACGTACGGTATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the percentage of antibiofilm activity against S. aureus BPA-12 (19.1%) and E. coli EPEC 4 (-1.3%).N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_1663 https://doi.org/10.48022/mbl.2206.060012022Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coliS15K20CCGGAATTCCTAATACGACTCCCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGATATTGAAAACGCGGCCGCGG81N/AssDNAStaphylococcus aureus (S. aureus) BPA-12 and Escherichia Coli (E. Coli) EPEC 4Whole cellInhibition of biofilm formation by folding into unique three-dimensional structures and attaching to a specific target.Showed the highest percentage of antibiofilm activity against E. coli EPEC 4 (15.4%).N/AN/AN/ATherapeuticsN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_1671 343290202022Targeted mesoporous silica nanoparticles for improved inhibition of disinfectant resistant Listeria monocytogenes and lower environmental pollutionA15-HP-AmTACTATCGCGGAGACAGCGCGGGAGGCACCGGGGATAGTA40N/AssDNAListeria Monocytogenes (ATCC 19115 and ATCC 7644)Whole cellBAC-encapsulated, aptamer-functionalized silica nanoparticles (AptBACNP) effectively killed only the target bacterium, L. monocytogenes, at lower doses, but not other bacteria.Inhibition (MIC value) of BAC resistant Listeria strains with 8 times less the usual disinfectant dose.N/AN/AN/ATherapeuticsN/A5'-Amidation (NH₂)MSNPs are slightly cytotoxic to erythrocytes and MCF-7 cells at concentrations more than 25 µg/ml.N/AN/AN/AN/A
ABdb_1672 358504612022Aptamer decorated emodin nanoparticles-assisted delivery of dermcidin-derived peptide DCD-1L: Photoactive bio-theragnostic agent for Enterococcus faecalis biofilm destructionAptamerTAGGGAAGAGAAGGACATATGATACTGGCCTTGACACCCTGTTGTGGCTTGATGACAATAACATTGACTAGTACATGACCACTTGA86N/AssDNAEnterococcus FaecalisWhole cellApt@EmoNp-DCD-1L that binds E. faecalis, enabling targeted delivery of emodin nanoparticles and DCD-1L for anti-biofilm activity.aPDT using Apt@EmoNp-DCD-1L caused ≈99.99% reduction of E. faecalis viability and strong biofilm disruption at sub-MIC concentrations (7.8 and 15.6 µM).N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-FAM Labeled and 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1679 350312532022Architecting of an aptasensor for the staphylococcus aureus analysis by modification of the screen-printed carbon electrode with aptamer/Ag-Cs-Gr QDs/NTiO2AptamerTCGGCACGTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC43N/AssDNAStaphylococcus aureus (S. aureus)Whole cellAn electrochemical aptasensor employing Apt/Ag–Cs-Gr QDs/NTiO2/SPCE was developed for the detection of S. aureus.Limit of Detection (LOD) of 3.3 CFU/mL and dynamic range of 10–5 × 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/ACompared with the initial current response, the aptsensor response changed less than 10% after 100 cycles’ continuous CV scans.N/AN/AN/A
ABdb_1698 358968422022Gold nanoparticles (AuNP)-based aptasensor for enteropathogenic Escherichia coli detectionS8-7CCGGAATTCCTAATACGACTCGGTTCCGGTAAGATTAGATCATAACGTATGGCTAGCGCCATATTGAAAACGCGGCCGCGG81N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellAn AuNP-based aptasensor was developed for the detection of enteropathogenic Escherichia coli.N/AN/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)17.18 nMBiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1699 358968422022Gold nanoparticles (AuNP)-based aptasensor for enteropathogenic Escherichia coli detectionS8-7.2GGTTCCGGTAAGATTAGATCATAACGTATGGCTAGCGCCATATTGAAAACGCGGCCGCGG60N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellAn AuNP-based aptasensor was developed for the detection of enteropathogenic Escherichia coli.LOD value of AuNP-based aptasensor after overnight incubation with a value of 10(5) CFU/mL.N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1700 358968422022Gold nanoparticles (AuNP)-based aptasensor for enteropathogenic Escherichia coli detectionS10-5N/AN/AN/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellAn AuNP-based aptasensor was developed for the detection of enteropathogenic Escherichia coli.LOD of AuNP-based aptasensor was achieved at overnight incubation with a value of 10(6) CFU/mL.N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)34.14 nMBiosensorN/AN/AN/AN/ABest CandidateN/AN/A
ABdb_1713 363545052022Simultaneous Amperometric Aptasensor Based on Diazonium Grafted Screen-Printed Carbon Electrode for Detection of CFP10 and MPT64 Biomarkers for Early Tuberculosis DiagnosisMPT64 aptamerTTTTTTTTTTGGGAGCTGATGTCGCATGGGTTTTGATCACATGA44N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinAmperometric dual aptasensor for the simultaneous detection of M. tb. secreted antigens CFP10 and MPT64 for the diagnosis of TB.Achieved a detection limit of 1.82 ng/mL and a linear range of 0.75–250 ng/mL for MPT64 antigen within 2.5 h.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1714 363545052022Simultaneous Amperometric Aptasensor Based on Diazonium Grafted Screen-Printed Carbon Electrode for Detection of CFP10 and MPT64 Biomarkers for Early Tuberculosis DiagnosisCFP10 aptamerN/AN/AN/AN/AMycobacterium Tuberculosis (M.Tb.)CFP10 antigenAmperometric dual aptasensor for the simultaneous detection of M. tb. secreted antigens CFP10 and MPT64 for the diagnosis of TB.Achieved a detection limit of 1.68 ng/mL and a linear range of 0.5–100 ng/mL for CFP10 antigen within 2.5 h.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1715 354482892022Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride NanosphereS. typhimurium AptTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDevelop an off-on fluorescence aptasensor for the detection of S. typhimurium and use HCNS-Cap for disinfection.The fluorescence assay showed a linear range of 30 to 3 × 10(4) CFU/mL and a detection limit of 13 CFU/mL. The bactericidal efficiency of HCNS-Cap (95.0%) within 12 h was better than that of HCNS (85.1%) and Cap (72.9%).N/AN/AN/ADiagnostic/TherapeuticsN/ACyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1716 362058282022Aptamer-AuNP-conjugated carboxymethyl chitosan-functionalized graphene oxide for colorimetric identification of Salmonella typhimuriumS. typhimurium AptamerGCGCTCGGCCTCCTCTGCCATCTCATTCGCGAGCC35N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop a novel aptamer-AuNP-conjugated carboxymethyl chitosan–functionalized graphene oxide (CMC/GO@Apt-Au NP) probe for the determination of S. typhimurium.Achieved a linear range from 10(2) to 10(7) CFU/mL with a limit of detection (LOD) of 10 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AThe relative response of aptasensor decreased to ~ 77% within 11 days of storage.N/AN/AN/A
ABdb_1717 346934712022Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12aAptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellAn electrochemical biosensor platform based on HCR-based CRISPR-Cas12a for specific detection of S. typhimurium.Selectively and sensitively quantify S. typhimurium in samples with detection limits of 20 CFU/mL and a linear range of 10-10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1718 360589392022Multiple amplification-based fluorometric aptasensor for highly sensitive detection of Staphylococcus aureusAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped a fluorometric aptasensor for the detection of S. aureus using magnetic nanoparticles (MNPs).The proposed fluorometric aptasensor displays LOD of (1.23 cfu/mL, a wide linear range (1 ~ 10(8) cfu/mL), and a fast detection speed (~ 1.5 h).N/AN/AN/ABiosensorN/A5'-Biotinylated (Biotin-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1719 349864392022Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readoutAptamerGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCG36N/AssDNAEscherichia Coli (E. Coli) O157: H7Whole cellDeveloped a potentiometric aptasensor by electrogenerated chemiluminescence (ECL) using SWCNTs-aptamer modified electrode to detect E. coli.Shows a highly sensitive response to E. coli O157: H7 in the linear range of 5-1000 CFU/mL with a low detection limit of 2 CFU/mL.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1724 352709992022Detection of E. coli Bacteria in Milk by an Acoustic Wave Aptasensor with an Anti-Fouling CoatingAptamerATCCGTCACACCTGCTCTACGGCGCTCCCCAACAGGCCTCTCCTTACGGCATATTATGGTGTTGGCTCCCGTAT74N/AssDNAEscherichia Coli (E. Coli) DH5αWhole cellDeveloped an electromagnetic piezoelectric acoustic sensor device (EMPAS) based on SiO2-MEG-NH2-Aptamer for the detection of E.coli. in milk samples.Selective measurement of E. coli in PBS and in cow’s milk samples down to limits of detection of 35 and 8 CFU/mL, respectively, with a linear range of 10–10(7) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1740 361089852022Naked-eye detection of Staphylococcus aureus in powdered milk and infant formula using gold nanoparticlesAnti-S. aureus aptamer (Apt1)TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (FPR3757 USA300)Whole cellDeveloped a colorimetric LSPR aptasensor using gold nanoparticles for the detection of S. aureus in milk and infant formula.Could be visually detected within 30 min, with detection limits of 7.5 × 10(4) CFU/mL and 8.4 × 10(4) CFU/mL in milk and infant formula, respectively.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1741 348029262022Rapid and selective detection of Bacillus cereus in food using cDNA-based up-conversion fluorescence spectrum copy and aptamer modified magnetic separationAptamerAGCAGCACAGAGGTCAGATGCCCCCCTTTTATCCGTCGGCATGATGTCTCCCGATCCGGTCCTATGCGTGCTA73N/AssDNABacillus CereusWhole cellDeveloped an aptansensor for Bacillus cereus detection based on up-conversion and magnetic nanomaterials (UCNPs-cDNA-Apt-MNPs complex).Display a linear range of 49-49 × 10(6) cfu/mL under the optimal conditions with a detection limit of 22 cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1742 361993432022Dual-mode sensor based on the synergy of magnetic separation and functionalized probes for the ultrasensitive detection of Clostridium perfringensDNA walker AptamerTTTTTTTTTTTTTTTTTTTTTACAGAGCACGGGAATGTTACTGCCTGTTCAACGGCAGTAACATTAGC68N/AssDNAClostridium PerfringensC. perfringens genomic DNADeveloped a dual-mode aptasensor using the synergy between fluorescent and electrochemical signals based on a DNA walker and hybridization chain reaction (HCR) for Clostridium perfringens detection.Displayed an excellent analytical performance for C. perfringens at a concentration of 1 to 10(8) CFU/g and a minimum concentration of 1 CFU/g in real samples.N/AN/AN/ABiosensorN/AFAM LabeledN/AN/AN/AN/AN/A
ABdb_1743 361920572022Aptamer-based colorimetric detection of methicillin-resistant Staphylococcus aureus by using a CRISPR/Cas12a system and recombinase polymerase amplificationAptamer 2CCTCCCTCCCTCCCTTTTTCCCACCCACCCACC33N/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellDeveloped an aptamer-based colorimetry sensor using a CRISPR/Cas12a system and recombinase polymerase amplification (RPA) for sensitive detection of MRSA.MRSA was detected as low as 8 CFU/mL and can be quantified with a linear range of 10 CFU/mL to 10(5) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1744 352752702022DNAzyme-controlled plasmonic coupling for SERS-based determination of Salmonella typhimurium using hybridization chain reaction self-assembled G-quadruplexAptAGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA40N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 1925)Whole cellDeveloped a SERS aptasensor based on hybridization chain reaction (HCR) self-assembled G-quadruplex DNAzyme (GQH DNAzyme)-controlled plasmonic coupling for S. typhimurium detection.Display a linear range from 5 to 5.0 × 10(5) cfu/mL and the limit of detection (LOD) of 4 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1745 https://doi.org/10.1016/j.snb.2021.1308392022A dual-mode aptasensor for foodborne pathogens detection using Pt, phenylboric acid and ferrocene modified Ti3C2 MXenes nanoprobeV.P-AptTTTTTTCACCCCACCTCGCTCCCGTGACACTAATGCTA38N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped a dual-mode aptasensor based on electrochemical and colorimetric modes using PBA-Fc@Pt@MXenes nanoprobe for the detection of V.P in shrimps.Showed a LOD and linear range of 5 CFU/mL and 10(1) to 10 (8) CFU/mL by electrochemical mode and 30 CFU/mL and 10(2) to 10 (8) CFU/mL by coulometric mode.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AThe electrochemical signal retained 89.6% of its original value after 2 weeks.N/AN/AN/A
ABdb_1746 https://doi.org/10.1016/j.snb.2021.1308792022Aptamer-conjugated magnetic Fe3O4@Au core-shell multifunctional nanoprobe: A three-in-one aptasensor for selective capture, sensitive SERS detection and efficient near-infrared light triggered photothermal therapy of Staphylococcus aureusAptGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CMCC 26003)Whole cellDeveloped an aptasensor based on Fe3O4@Au nanocomposites (NCs) for capture, SERS detection, and photothermal therapy (PTT) of S. aureus.The detection limit is 25 cfu/mL, the cell capture efficiency (CCE) is as high as 68% and it has a high photothermal conversion efficiency of 39.28%.N/AN/AN/ADiagnostic/TherapeuticsN/A5'-ThiolatedThe cytotoxicity of Fe3O4@Au-Apt NCs to S. aureus is negligible.The SERS intensity of S. aureus on the Fe3O4@Au-Apt NCs remains almost unchanged within 12 days.N/AN/AN/A
ABdb_1747 375129482023Alpha-Gal Bound Aptamer and Vancomycin Synergistically Reduce Staphylococcus aureus Infection In VivoαSA31ATGATCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA49N/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA) (ATCC 33591)Whole cellαSA31 rescue α-1, 3-galactosyltransferase (−/−) knockout (GTKO) mice from induced MRSA sepsis.3.5-fold more (p < 0.05) pretreated MRSA were phagocytized when also treated with αSA31 and 7/12 mice treated with vancomycin plus αSA31 survived in-vivo.N/AN/AN/ATherapeuticsN/A5'-α-gal and 5'-Amidation (NH₂-(CH2)6)No toxicity was observed, even at 10,000 µg/kg/day.αSA31NH2 was significantly more stable (p < 0.01) in human serum, as no degradation was observed after 24 h at 37°C.N/A5.222 h in Human serum.N/A
ABdb_1768 https:doi.org10.1016j.snb.2023.1342182023Dual-mode sensing platform based on aptamer-tunable catalytic activity of mesoporous polydopamine/MnO2 nanozymes for detecting S. aureusSA31GCAATGGTACGGTACTTCCTCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGACAAAAGTGCACGCTACTTTGCTA87N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellColourimetric-electrochemical sensing platform to detect S. aureus based on a dual-modal strategy.Shows a low detection limit in 3 CFU/mL with a linear range between 5 and 10(7) CFU/mL and in a real sample with the recovery of 95.15%− 115.28%.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1770 381570412023A point-of-care aptasensor based on the upconversion nanoparticles/MoS2 FRET system for the detection of Pseudomonas aeruginosa infectionAptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellDevelop an aptasensor based on FRET between UCNPs-apt/MoS2 for rapid detection of P. aeruginosa.Exhibit a wide linear detection range 8.7 × 10 ~ 8.7 × 10(7) cfu/mL, and a low limit of detection (LOD) of 15.5 cfu/mL within 1.5 h.N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1771 https://doi.org/10.1016/j.colsurfa.2023.1319552023MoS2 nanosheets based label-free colorimetric aptasensor for Escherichia coli O157: H7 detectionAptamerATCCGTCACACCTGCTCTGTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCGTGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDeveloped colorimetric aptasensor based on aptamer-functionalized MoS2 nanosheets for the detection of E. coli O157: H7.Detection of E. coli O157: H7 with a Limit of detection (LOD) of 116 CFU/mL and a linear concentration range of 500–5000 CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1772 372867532023An ultrasensitive aptamer-based fluorescent on/off system for trace amount evaluation of Yersinia enterocolitica in food samplesAptamerAGCAGCACAGAGGTCAGATGCTTGGTTCCACCGTACTGACTGTAGTAAATCTGATCACTCCTATGCGTGCTACCGTGAA79N/AssDNAYersinia Enterocolitica (PTCC 1786)Whole cellFluorescence-based aptasensor using graphene oxide (GO) as a quenching platform was developed for the detection of Y. enterocolitica.Exhibited a wide linear response in the concentration range 10 to 1.0 × 10(9) CFU/mL and the limit of detection (LOD) was 3 CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AFAM-aptamer-GO shows better stability in acidic environments, and pH 7 showed the greatest increase. It shows no obvious changes in fluorescence intensity within 60 days.N/AN/AN/A
ABdb_1773 379216342023Aptamer and DNAzyme-Functionalized Cu-MOF Hybrid Nanozymes for the Monitoring and Management of Bacteria-Infected WoundsAptamerGGGAAAGGGAAAGGGAAAGGG-TTTTTT-ATCCAGACGTGACGCAGCATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTGTGGACACGGTGGCTTAGTA102N/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA)MRSA surface componentsG-quadruplex/hemin DNAzyme-aptamer probes and tannic acid-chelated Au nanoparticle (Au-TA)-decorated Cu-based MOF nanosheets (termed GATC) with triple-enzyme activities were developed for visual detection and efficient antibacterial therapy.~99.7% MRSA inactivation at 3 μg/mL, with simultaneous colorimetric monitoring of infection.N/AN/AN/ATherapeuticsN/A5'-ThiolatedAt 50 μg/mL GATC, the cells showed high viability (>90%), indicating negligible cytotoxicity and good cytocompatibility. The hemolysis rate of the GATC nanozyme was low (<5%).N/AN/AN/AN/A
ABdb_1774 372441922023High-throughput, highly sensitive and rapid SERS detection of Escherichia coli O157:H7 using aptamer-modified Au@macroporous silica magnetic photonic microsphere arrayAptamerTGGTCGTGGTGAGGTGCGTGTATGGGTGGTGGATGAGTGTGTGGC45N/AssDNAEscherichia Coli (E. Coli) O157:H7 (CICC 21530)Whole cellAn aptamer-Au@MMSPM SERS array biochip method was developed for the detection of E. coli O157:H7.Gave a wide linear detection range (10–10(6) CFU/mL) and low limit of detection (2.20 CFU/mL) for E. coli O157:H7.N/AN/AN/ABiosensorN/A5'-Thiolated (SH-C12) (of Aptamer 1) and 3'-Amidation (NH₂-(CH2)6) (for Aptamer 2)N/AN/AN/AN/AN/A
ABdb_1775 374491202023Colorimetric aptasensor for detection of Bacillus cytotoxicus spores in milk and ready-to-use foodBAS-6RATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGATGGTGTTGGCTCCCGTAT71N/AssDNABacillus Cereus ssp. cytotoxicus (NVH 391-98A)B. cytotoxicus sporesDevelop a colorimetric assay employing AuNPs and magnetic particles (MPs) for the detection of Bacillus cytotoxicus spores in food.Achieving the naked-eye limit of detection as low as 10(2) cfu/mL in water and milk, and 10(4) cfu/mL in mashed potatoes.N/AN/AN/ABiosensorN/A5′-Thiolated and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1776 369069922023An ultrasensitive electrochemical aptasensor using Tyramide-assisted enzyme multiplication for the detection of Staphylococcus aureusSA37GCTTCCAGCTTATTGAATAAAGACGGGGGGGGGGACCGGCGTATGAGTGAAGATGGGGGCGCTGAAGCGCGGAAGC76N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellAn electrochemical aptasensor based on the tyramide signal amplification (TSA) technology was developed for the detection of S. aureus.Achieved a limit of detection (LOD) of 3 CFU/mL in buffer and 8 CFU/mL in both tap water and beef broth.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1777 368320382023Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu2WO4(OH)2 and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus DetectionAptTCGGCACGTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC43N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an aptasensing Platform based on a Green Nanocomposite consisting of Spongin Decorated Cu2WO4(OH)2 and AgNPs for Staphylococcus aureus detection.Measured S. aureus under a linear concentration range of 10–10(8) CFU/mL and a limit of quantification and detection of 12 and 1 CFU/mL, respectively.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1781 373272072023FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected AptamersA14ACTGTCCACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT54N/AssDNAStaphylococcus aureus (S. aureus)Iron-regulated Surface Determinant Protein A (IsdA)Develop a FRET-based aptasensor for single-molecule detection of IsdA.LOD value of A14 DNA was 485 pM.N/AFluorescence Resonance Energy Transfer Assay (FRET)4 ± 2 nMDetectionN/A5'-Cyanine3 (Cy3) Labeled or 5'-Biotinylated and 5′ end by six nucleotides (ACT GTC)N/AN/AN/AN/AN/A
ABdb_1782 365492132023Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tagsApt1GCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellA SERS platform using aptamers modified-Fe3O4@SiO2-Au NCs was developed for simultaneous detection of E. coli and S. aureus.The detection limit for E. coli was as low as 10 cfu/mL, and the linear concentration ranged from 10(1)-10(8) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) or 5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1783 365492132023Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tagsApt2GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CMCC 26003)Whole cellA SERS platform using aptamers modified-Fe3O4@SiO2-Au NCs was developed for simultaneous detection of E. coli and S. aureus.The detection limit for S. aureus was as low as 10 cfu/mL, and the linear concentration ranged from 10(1)-10(8) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂) or 5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1784 https://doi.org/10.1016/j.snb.2023.1335542023A novel Aptamer-induced CHA amplification strategy for ultrasensitive detection of Staphylococcus aureus and NIR-triggered photothermal bactericidal Activity based on aptamer-modified magnetic Fe3O4@AuNRsAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a Fe3O4 @AuNRs-aptasensor based on CHA for ultrasensitive detection of S. aureus.Achieved a linear response ranging from 10(2) to 10(6) CFU/mL and a detection limit of 10(1) CFU/mL under optimal conditions.N/AN/AN/ABiosensorN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1785 https://doi.org/10.1016/j.cclet.2022.1081022023Rapid detection of pathogenic bacteria based on a universal dual-recognition FRET sensing system constructed with aptamer-quantum dots and lectin-gold nanoparticlesAptamerATCCGTCACACCTGCTCTATCAAATGTGCAGATATCAAGACGATTTGTACAAGATGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) (CICC 21530)Whole cellDeveloped a dual-recognition-based sandwich FRET sensor using Aptamer-QDs and Con A-AuNPs for the detection of E. coli.Achieved a linear detection range from 10(2) cfu/mL to 2 × 10(8) cfu/mL with the detection limit of 45 cfu/mL for E. coli, and LOD in the milk and orange juice was 300 cfu/mL and 200 cfu/mL, respectively.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1786 https://doi.org/10.1016/j.snb.2023.1337452023Dual recognition strategy for the rapid and precise detection of Bacillus cereus using post-modified nano-MOF and aptamerAptamerAGCAGCACAGAGGTCAGATGCCCCCCTTTTATCCGTCGGCATGATGTCTCCCGATCCGGTCCTATGCGTGCTA73N/AssDNABacillus Cereus (CICC 10041)Whole cellA dual-recognition strategy using a fluorescent probe, FcMBL-coated nano-MIL-53(Al)-NH2, for detecting B. cereus was developed.Shows a linear range of 20–2 × 10(8) CFU/mL with a limit of detection of 4 CFU/mL within 60 min.N/AN/AN/ABiosensorN/A5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1787 371964082023A SERS bioassay based on vancomycin-modified PEI-interlayered nanocomposite and aptamer-functionalized SERS tags for synchronous detection of Acinetobacter baumannii and Klebsiella pneumoniaeK2AATCAGGCTCAGCATGGAGTTGCGAGGCCAATATCCGGTTAAGCG45N/AssDNAKlebsiella Pneumoniae (KP)Whole cellA double-edged sword SERS aptasensor was developed using SPION-PEI-Au-Van nanocomposites for synchronous detection of K. pneumoniae and A. baumannii in food and clinical samples.Shows a limit of detection was 10 cells/mL with a linear range of 50-10(5) cells/mL for both KP and AB.N/AN/A5.377 nMBiosensorN/A5'-Amidation (NH₂-(CH2)6) and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1788 371964082023A SERS bioassay based on vancomycin-modified PEI-interlayered nanocomposite and aptamer-functionalized SERS tags for synchronous detection of Acinetobacter baumannii and Klebsiella pneumoniaeABACAGCACCACAGACCACATATCACATGCTGTCGCCTTGCGATATCAATTCCAGTGATGTTTGTCTTCCTGCC72N/AssDNAAcinetobacter BaumanniiWhole cellA double-edged sword SERS aptasensor was developed using SPION-PEI-Au-Van nanocomposites for synchronous detection of K. pneumoniae and A. baumannii in food and clinical samples.Shows a limit of detection was 10 cells/mL with a linear range of 50-10(5) cells/mL for both KP and AB.N/AN/A6.8 nMBiosensorN/A5'-Cyanine5 (Cy5) Labeled and 5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1792 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt (mono-apt)GAGAGAGAATATAAGGGAAAAAAAAAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG82N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.N/AN/AEnzyme-Linked Immunosorbent Assay (ELISA)389.63 nM (with mono-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1793 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt1 (multi-apt)GAAGTGTACGTAGCCTGATTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1794 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt2 (multi-apt)TCGTCTACACTACTGCTCTTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1795 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt3 (multi-apt)TCTCCAATGACGTACCTGTTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1796 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt4 (multi-apt)GTTGCAGTACTCTACGAGTTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1797 https://doi.org/10.1016/j.snb.2022.1328602023A HCR based multivalent aptamer amplifier for ultrasensitive detection of SalmonellaApt5 (multi-apt)TGTACTGGACAAGTGACGTTAAAAAAAAAAAAGTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG77N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a hybridization chain reaction (HCR) based multivalent aptamer (multi-Apt) as an effective signal amplifier for the sensitive detection of Salmonella.Could detect Salmonella with multi-apt as low as 7 cfu/mL with a broad detection range of 10 to 10(7) cfu/mL.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.72 nM (with multi-apt)BiosensorN/A5'-Biotinylated and 5'-FAM LabeledN/AThe signals generated by the multi-Apt amplifier with different storage times at 4°C have remained unchanged over the past month.N/AN/AN/A
ABdb_1798 371870622023A universal bacterial sensor created by integrating a light modulating aptamer complex with photoelectrochemical signal readoutPeptidoglycan aptamerGCAGTTGCGGGACAGGGAGTGCGCTGCTCCCC32N/AssDNAEscherichia Coli (E. Coli)PeptidoglycanDeveloped a signal-on photoelectrochemical biosensor for detecting peptidoglycan.Demonstrate a limit-of-detection of 82 pg/mL (13 pM) in buffer and 239 pg/mL (37 pM) in urine for peptidoglycan and 1913 CFU/mL for Escherichia coli in urine.N/AN/A0.415 ± 0.047 μMBiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1799 369619212023Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell LevelS. aureus aptamerTCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellAn electrochemical sandwich sensor was developed for the detection of a single bacterial cell based on dual recognition by the bacteria-imprinted polymer film (BIF) and aptamer (Au@Fc-Apt).The sensor could detect as low as 10 CFU/mL in a milk sample and 1 CFU/mL in PBS with a linear range from 10 to 10(5) CFU/mL for S. aureus.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AAfter storage at 4°C for 17 days, the sensor still retained about 92% of the initial detection signal.N/AN/AN/A
ABdb_1800 https://doi.org/10.1002/celc.2023002572023Rolling Circle Amplification/G-Quadruplex-Based Dual-Signal Ratiometric Electrochemical Aptasensor for Ultrasensitive Detection of Pathogenic BacteriaAptamer (P1)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA87N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923 and ATCC 29213)Whole cellDeveloped a ratiometric dual-signal electrochemical biosensor for ultrasensitive detection of S. aureus based on an aptamer recognition-induced rolling circle amplification (RCA)/G-quadruplex strategy.Showed a good linear relationship between 10–10(6) CFU/mL with a detection limit of 10 CFU/mL.N/AN/AN/ABiosensorN/AFerrocene (Fc) conjugatedN/AN/AN/AN/AN/A
ABdb_1809 376199022023Development of a label-free impedimetric aptasensor for the detection of Acinetobacter baumannii bacteriaAptamerACAGCACCACAGACCACATATCACATGCTGTCGCCTTGCGATATCAATTCCAGTGATGTTTGTCTTCCTGCC72N/AssDNAAcinetobacter Baumannii (ATCC 19606)Whole cellAn electrochemical aptasensor was developed using an aptamer immobilized on the surface of a CSPE modified with the nanocomposite Fe3O4@SiO2@Glyoxal (Gly) for A. baumannii detection.Specifically detect A. baumannii in the concentration range from 1.0 × 10(3)–1.0 × 10(8) CFU/mL and with a detection limit of 150 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AThe current response from 0 day to 28 days was 94.3%, 90%, and 86.5% of the primary Rct response of the aptasensor remained at 4°C after 14, 21 and 28 days, respectively.N/AN/AN/A
ABdb_1811 https://doi.org/10.3390/fishes81004772023A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in AquacultureAptamerGAGAGAGAATATAAGGGAAAAAAAATCAGTCGCTTCGCCGTCTCCTTCGGGGGCGCGGTGAGGGGCTGCACAAGAGGGAGGCACAAGAGGGAGACCCCAGAGGG104N/AssDNAVibrio Alginolyticus (ATCC 17749)Whole cellDeveloped a method using an HCR-based multivalent aptamer (multi-Apt) for the detection of Vibrio alginolyticus.Obtained a linear range from 10 to 10(7) CFU/mL, and the limit of detection (LOD) is 3 CFU/mL.N/AN/AN/ADetectionN/ASA-HRP or 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1812 378937442023A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent AptamerApt1GAAGTGTACGTAGCCTGATTCTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG60N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric/fluorescent dual-mode method based on a DNA-nanotriangle programmed multivalent aptamer (NTri-Multi-Apt) for the detection of Salmonella.Achieved a linear range of 1.0 × 10(2)–1.0 × 10(7) CFU/mL and LODs of 316 and 60 CFU/mL for colorimetric and fluorescent detection, respectively.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.89 nMBiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1813 378937442023A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent AptamerApt2TACTGCTCTTCTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG50N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric/fluorescent dual-mode method based on a DNA-nanotriangle programmed multivalent aptamer (NTri-Multi-Apt) for the detection of Salmonella.Achieved a linear range of 1.0 × 10(2)–1.0 × 10(7) CFU/mL and LODs of 316 and 60 CFU/mL for colorimetric and fluorescent detection, respectively.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.89 nMBiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1814 378937442023A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent AptamerApt3CGTACCTGTTCTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG50N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a colorimetric/fluorescent dual-mode method based on a DNA-nanotriangle programmed multivalent aptamer (NTri-Multi-Apt) for the detection of Salmonella.Achieved a linear range of 1.0 × 10(2)–1.0 × 10(7) CFU/mL and LODs of 316 and 60 CFU/mL for colorimetric and fluorescent detection, respectively.N/AEnzyme-Linked Immunosorbent Assay (ELISA)11.89 nMBiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1815 https://doi.org/10.1016/j.cej.2023.1430992023Design nanoprobe based on DNA tetrahedron supported hybridization chain reaction and its application to in situ analysis of bacteriaAptamer (AA)AGTGCGCAGCTAGCAAGGCCTTTTTTTTTTGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA118N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 6538)Whole cellDeveloped a nanoprobe (DTAAT) based on DNA tetrahedral supported hybridization chain reaction (HCR) for in situ analysis of SA.Detect SA with the detection limit as low as 26 CFU/mL and linear range of 50-5×10(6) CFU/mL.N/AN/AN/ABiosensorN/A5'-FAM Labeled and 3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1816 364936742023Sandwich-Type Electrochemical Aptasensor for Highly Sensitive and Selective Detection of Pseudomonas Aeruginosa Bacteria Using a Dual Signal Amplification StrategyF23CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 15442)Whole cellDeveloped a sandwich-type electrochemical aptasensor using a carbon screen-printed electrode (MIL101(Cr)/MWCNT) and AgNPs/C-g-C3N4/Apt for P. aeruginosa detection.Display a limit of detection of 1 CFU/mL and a linear range of 10 to 10(7) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AThe DPV current intensity of the aptasensor for 10(5) CFU/mL of P. aeruginosa was approximately 92.4% of its initial signals after 21 days stored at 4°C.N/AN/AN/A
ABdb_1817 367057342023A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS techniqueS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (CICC 10473)Whole cellDeveloped an assembled aptasensor based on Fe3O4@Au@Ag nanocomposites for the detection of various pathogenic bacteria.Showed a linear range of 10-10(7) CFU/mL, with a detection limit of 5.70 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1818 367057342023A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS techniqueE. coli aptamerCAGCTCAGAAGCTTGATCCTACCAGTAGACTTTCAACTTTACTGCCATCGTGTGCCCTAAGACTCGAAGTCGTGCATCTG80N/AssDNAEscherichia Coli (E. Coli) (CICC 10372)Whole cellDeveloped an assembled aptasensor based on Fe3O4@Au@Ag nanocomposites for the detection of various pathogenic bacteria.Showed a linear range of 10-10(7) CFU/mL, with a detection limit of 2.10 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1819 367057342023A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS techniqueS. flexneri aptamerCAGCAACACTGCAACACTGTATAGTCCTGTGTGCC35N/AssDNAShigella Flexneri (CGMCC 1.1868)Whole cellDeveloped an assembled aptasensor based on Fe3O4@Au@Ag nanocomposites for the detection of various pathogenic bacteria.Showed a linear range of 10-10(7) CFU/mL, with a detection limit of 2.72 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1820 367057342023A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS techniqueP. aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (CICC 21636)Whole cellDeveloped an assembled aptasensor based on Fe3O4@Au@Ag nanocomposites for the detection of various pathogenic bacteria.Showed a linear range of 10-10(7) CFU/mL, with a detection limit of 3.84 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1821 367057342023A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS techniqueS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an assembled aptasensor based on Fe3O4@Au@Ag nanocomposites for the detection of various pathogenic bacteria.Showed a linear range of 10-10(7) CFU/mL, with a detection limit of 2.27 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1822 367057342023A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS techniqueL. monocytogenes aptamerTACTATCGCGGAGACAGCGCGGGAGGCACCGGGGA35N/AssDNAListeria Monocytogenes (CICC 21635)Whole cellDeveloped an assembled aptasensor based on Fe3O4@Au@Ag nanocomposites for the detection of various pathogenic bacteria.Showed a linear range of 10-10(7) CFU/mL, with a detection limit of 3.16 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1823 361669242023Ultrasensitive multicolor electrochromic sensor built on closed bipolar electrode: Application in the visual detection of Pseudomonas aeruginosaAptamerTTTTTCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG65N/AssDNAPseudomonas Aeruginosa (ATCC 10145)Whole cellAn ultrasensitive multicolour electrochromic platform based on a closed bipolar electrode (BPE) was developed for visual sensing of P. aeruginosa.The detection limit was as low as 0.33 CFU/mL, and the linear range was 10(0)-10(8) CFU/mL within 30 min by the naked eye.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1824 https://doi.org/10.1016/j.microc.2023.1086052023A SERS biosensor based on aptamer-based Fe3O4@SiO2@Ag magnetic recognition and embedded SERS probes for ultrasensitive simultaneous detection of Staphylococcus aureus and Escherichia coliS. aureus aptamer (P1)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (BNCC 186335)Whole cellDeveloped a SERS biosensor based on aptamer-modified Fe3O4@SiO2@Ag and Au-MPBA/DTNB@Ag for simultaneous detection of S.aureus and E.coli.Rapidly detect S. aureus with the limit of detection (LOD) of 1 CFU/mL in 20 min and a linear range from 10(1) ∼ 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1825 https://doi.org/10.1016/j.microc.2023.1086052023A SERS biosensor based on aptamer-based Fe3O4@SiO2@Ag magnetic recognition and embedded SERS probes for ultrasensitive simultaneous detection of Staphylococcus aureus and Escherichia coliE. coli aptamer (P2)ATCCGTCACACCTGCTCTACTGGCCGGCTCAGCATGACTAAGAAGGAAGTTATGTGGTGTTGGCTCCCGTAT72N/AssDNAEscherichia Coli (E. Coli) (BNCC 336902)Whole cellDeveloped a SERS biosensor based on aptamer-modified Fe3O4@SiO2@Ag and Au-MPBA/DTNB@Ag for simultaneous detection of S.aureus and E.coli.Rapidly detect E. coli with the limit of detection (LOD) of 1 CFU/mL in 20 min and a linear range from 10(1) ∼ 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1826 https://doi.org/10.1016/j.microc.2023.1086812023An electrochemical and colorimetric dual-mode aptasensor for Staphylococcus aureus based on a multifunctional MOF and magnetic separation techniqueS. aureus AptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellDeveloped a colorimetric and electrochemical dual-mode aptasensor based on ssDNA-Au/CuMOF and MB-Apt for S. aureus detection.Display a broad linear range from 10 to 10(8) CFU/mL, with colorimetric minimum resolution of 48 CFU/mL, and electrochemical detection limit of 5 CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1827 373112992023Joint concanavalin A-aptamer enabled dual recognition for anti-interference visual detection of Salmonella typhimurium in complex food matricesS. typhimurium aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellA dual recognition platform based on Concanavalin A (Con A)-aptamer joint strategy for sensitive determination of S. typhimurium.Exhibited linear range of 7.0 × 10(1) ∼ 7.0 × 10(9) CFU/mL, along with a detection limit as low as 23 CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1828 374666982023A signal-off aptasensor for the determination of Acinetobacter baumannii by using methylene blue as an electrochemical probeAptACAGCACCACAGACCACATATCACATGCTGTCGCCTTGCGATATCAATTCCAGTGATGTTTGTCTTCCTGCC72N/AssDNAAcinetobacter Baumannii (ATCC 19606)Whole cellDeveloped an electrochemical aptasensor with Apt/MWCNT@Fe3O4@SiO2-Cl/CSPE nanocomposite to detect A. baumannii.Display a linear range of 10.0–1.0 × 10(7) CFU/mL and a detection limit of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AThe current density of the aptasensor for 10(5) CFU/mL of A. baumannii was approximately 92.3% of its initial signals after 21 days.N/AN/AN/A
ABdb_1830 382503552024Rapid Detection and Identification of Vancomycin-Sensitive Bacteria Using an Electrochemical Apta-SensorApt1TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (RN4220)Whole cellA portable electrochemical biosensor was developed for the identification of Gram-positive bacteria based on a vancomycin-modified screen-printed carbon electrode.Demonstrated that capture was achieved in 10 min, with a limit of detection of only 2.7 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1831 382503552024Rapid Detection and Identification of Vancomycin-Sensitive Bacteria Using an Electrochemical Apta-SensorSp14AGCAGCACAGGGTCAGATGATATGTTTACGCCAGTGGTATTATTGGGGTTGATATGTCACCTATGCGTG69N/AssDNABacillus Cereus (ATCC 14579)Whole cellA portable electrochemical biosensor was developed for the identification of Gram-positive bacteria based on a vancomycin-modified screen-printed carbon electrode.Demonstrated that capture was achieved in 10 min, with a limit of detection of only 2.4 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1838 https://doi.org/10.1002/adfm.2024034402024Next-Generation Wound Care: Aptamer-Conjugated Polydiacetylene/Polyurethane Nanofibrous Biosensors for Selective In Situ Colorimetric Detection of PseudomonasF23CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellDeveloped an aptamer-modified polydiacetylene-based electrospun nanofibrous wound dressing for the detection of Pseudomonas aeruginosa.The LOD for the aptamer-modified membrane P-F23 was 1.42 × 10(6) CFU/cm^2.N/AN/AN/ABiosensorN/AAmidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1839 https://doi.org/10.1002/adfm.2024034402024Next-Generation Wound Care: Aptamer-Conjugated Polydiacetylene/Polyurethane Nanofibrous Biosensors for Selective In Situ Colorimetric Detection of PseudomonasSt21Lp17AAGCGTCGGTGTTCTATCGGTAGTTGACACCGACGCCT38N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellDeveloped an aptamer-modified polydiacetylene-based electrospun nanofibrous wound dressing for the detection of Pseudomonas aeruginosa.The LOD for the aptamer-modified membrane P-St21Lp17 was 4.46 × 10(6) CFU/cm^2.N/AN/AN/ABiosensorN/AAmidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1840 387706562024Sensitive and on-Site Detection of Staphylococcus aureus Based on CRISPR/Cas 13a-Assisted Chemiluminescence Resonance Energy TransferS. aureus AptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDevelop a CRISPR/Cas 13a-assisted CRET-based strategy for the detection of S. aureus in real samples.Successfully detected S. aureus in drinking water and milk with detection limits of 20 and 30 cfu/mL, respectively, within the recovery of 90.07–105.50%.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1841 397279012024Competitive Electrochemical Apta-Assay Based on cDNA-Ferrocene and MXenes for Staphylococcus aureus Surface Protein A DetectionAPTATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Staphylococcus aureus Protein A (SpA)Developed a competitive aptasensor based on a ferrocene (Fc)-labeled cDNA hybridized (cDNA-Fc S13) on a specific aptamer (APT) for PrA in the presence of MXene nanosheets for the indirect detection of S. aureus.Displayed a linear range of 10–125 nM, an LOD of 3.33 nM, and a response time under 40 min.N/AN/AN/ABiosensorN/A3'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1842 395332982024Rapid detection of Brucella cells using a gold nanoparticle-based aptasensor via a simple colorimetric methodB. melitensis-specific aptamerGAGAGTAAAGGCCATCGGCGGCCATTTATGTTGTACCC38N/AssDNABrucella Melitensis strain Rev. 1 vaccine and Brucella Abortus strain RB51 vaccineWhole cellDetection of Brucella cells using a biosensor, based on gold nanoparticles (GNPs) and a specific aptamer, via a colorimetric reaction.Able to detect 1.5 × 10(1) CFU/mL with a linear range of 1.5 × 10(1)-1.5 × 10(8) CFU/mL of the bacterial cells.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1843 392126952024Visual fluorescence detection of Listeria monocytogenes with CRISPR-Cas12a aptasensorAptamerATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT47N/AssDNAListeria Monocytogenes (ATCC 19115)Whole cellDeveloped an aptasensor utilizing carboxylated magnetic beads and Cas12a to detect L. monocytogenes.Demonstrates excellent sensitivity towards L. monocytogenes, with a lowest detection limit (LOD) of 57.15 CFU/mL and a linear range of 4×10(2) to 4×10(7) CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1844 381509652024Colourimetric and SERS dual-mode aptasensor using Au@Ag and magnetic nanoparticles for the detection of Campylobacter jejuniONS-23GCAAGATCTCCGAGATATCGTGCTGGGGGGTGGTTTGTTTGGGTCGGTTGTTTTGGTTGGGCTGCAGGTAATACGTATACT81N/AssDNACampylobacter Jejuni (ATCC 33291)Whole cellDeveloped a dual-mode aptasensor using aptamer-conjugated Au@Ag NPs for the effective detection of C. jejuni.Exhibit a wider linear range (1.8 × 10(1)–10(8) CFU/mL and a lower limit of detection of 6 CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (for apt-1) and 5′–NH2 (for apt-2)N/AN/AN/AN/AN/A
ABdb_1845 380068172024Electrocatalytic aptasensor for bacterial detection exploiting ferricyanide reduction by methylene blue on mixed PEG/aptamer monolayersAptamerATCCGTCACACCTGCTCTACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTATGGTGTTGGCTCCCGTAT73N/AssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Developed a disposable electrocatalytic “off–on” sensor for E. coli on Au SPE.Exhibited a linear range of 10 to 1000 CFU/mL and LOD of 10 CFU/mL E. coli in 30 min.N/AN/AN/ABiosensorN/A3'-Thiolated (thiol-C6)N/AN/AN/AN/AN/A
ABdb_1846 381692792024"Five birds one stone" tri-modal monitoring driven lab-on-magnetic aptasensor for accurate pathogen detection and enhanced germicidal applicationAnti-S. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 65389)Whole cellA fluorescence-colorimetric-photothermal tri-modal sensing platform was developed for the detection of S. aureus by apt/KCl@CDs and magnetic multi-walled carbon nanotube composites (M-MWCNTs).The detection limits of fluorescence, colorimetric and photothermal models were 4.81 cfu/mL, 3.40 cfu/mL and 6.74 cfu/mL, respectively with the same linear range of 10 - 1.0 ×10(7) cfu/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1847 380402922024One-pot rapid visual detection of E. coli O157:H7 by label-free AuNP-based plasmonic-aptasensor in water sample(PGM) aptamerCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43895)Whole cellA label-free biosensor was developed using an aptamer and single-pot Au nanoparticle (AuNP) for the detection of E. coli O157:H7.Could identify target bacteria with as few as 250 CFU/ml in 15 min or less.N/AN/AN/ABiosensorN/A5'-Dodeca-G (G12)N/AAfter five days (stored at 4°C) from synthesizing PG-apt-AuNPs, its performance in detecting 10(5) CFU/ml of E. coli O157:H7 is similar to that of newly synthesized biosensor.N/AN/AN/A
ABdb_1848 384525912024Aptamer-mediated double strand displacement amplification with microchip electrophoresis for ultrasensitive detection of Salmonella typhimuriumAptamer (Apt)CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT59N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-mediated double SDA-MCE method for ultrasensitive detection of S. typhimurium.Achieved a linear range of 30–1.0 × 10(5) CFU/mL and the limit of detection for S. typhimurium down to 6 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1849 https://doi.org/10.1016/j.biosx.2023.1004362024DNA origami-enhanced binding of aptamers to Staphylococcus aureus cellsPA#2/8 [1–58]ATACCAGCTTATTCAATTAGCAACATGAGGGGGATAGAGGGGGTGGGTTCTCTCGGCT58N/AssDNAStaphylococcus aureus (S. aureus) SA5Staphylococcus aureus Protein A (SpA)Developed a DNA origami-based biosensor for the detection of SA.Five-times higher affinity of the aptamer-functionalized DNA origami compared to pure aptamers.N/AEnzyme-Linked Oligonucleotide Assay (ELONA)160 ± 9 nMBiosensorN/A5'-PolyA (21A) or PolyT (21 T)N/AN/AN/AN/AN/A
ABdb_1850 https://doi.org/10.1016/j.electacta.2024.1442402024Label-free electrochemical aptasensor for detection of Acinetobacter baumannii: Unveiling the kinetic behavior of reduced graphene oxide v/s graphene oxideAptamerACAGCACCACAGACCACATATCACATGCTGTCGCCTTGCGATATCAATTCCAGTGATGTTTGTCTTCCTGCC72N/AssDNAAcinetobacter Baumannii (ATCC BAA-2800)Whole cellDeveloped an electrochemical aptasensor that employed a screen-printed carbon electrode modified with both graphene oxide (GO) and reduced graphene oxide (rGO) for the detection of AB.Achieved an impressive limit of detection of 10 CFU/mL and a linear range of 10 to 10(9) CFU/mL.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AThe current response of aptasensor exhibited negligible changes in the CV signal even after the 4-week storage period.N/AN/AN/A
ABdb_1851 381804962024Development of an electrochemical sensitive aptasensor based on a zeolite imidazolate framework-8 and gold nanoparticles for the determination of Staphylococcus aureus bacteriaAptamerTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC46N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an electrochemical aptasensor based on a glassy carbon electrode (GCE) modified with zeolite imidazolate framework-8 (ZIF 8) and gold nanoparticles (AuNPs) for the detection of S. aureus.Showed a linear response in the concentration range of 1.5 × 10(1) to 1.5 × 10(7) CFU mL−1 and a detection limit of 3.4 CFU/mL.N/AN/A(9.04 ± 1.27) × 10(−1) nMBiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AResponse of the proposed aptasensor reached 98.9%, 94.3%, and 92.8% of the initial response on the first, second, and third days, respectively, when kept at 4°C.N/AN/AN/A
ABdb_1852 377516322024A novel paper-based electrochemiluminescence biosensor for non-destructive detection of pathogenic bacteria in real samplesAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTGCTAACCCCCCTTAATCCCCCC104N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a paper-based electrochemiluminescence (ECL) aptasensor based on a Ru-MOF-5 NFs modified Indium tin oxide (ITO) electrode for the detection of S. aureus.Exhibited a linear range from 5 to 10(8) CFU/mL, and a limit of detection (LOD) of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AAfter 9 days at 4°C, the ECL signal of aptsensor retained 95.8% of its initial value, suggesting satisfactory storage stability.N/AN/AN/A
ABdb_1853 https://doi.org/10.1016/j.microc.2024.1114282024Colorimetric and fluorescent dual-mode detection of Escherichia coli using Fe3O4 nanoparticle coated with fluorescein-labeled aptamerE. coli aptamerATCCGTCACACCTGCTCT-ACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA-TGGTGTTGGCTCCCGTAT73N/AssDNAEscherichia Coli (E. Coli) BL21 strainOuter membrane protein Ag1 (E. coli OMP Ag1)Developed a dual-mode colorimetric and fluorescence assay employing Fe3O4 nanoparticles (NPs) coated with FAM-Apt for the identification of E. coli.Acheived an impressive limit of detection (LOD) of 10 CFU/mL in the colorimetric mode and 6 CFU/mL in the fluorescence mode.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1854 390963252024Colorimetric aptasensor for Listeria monocytogenes detection using dual functional Fe3O4@MIL-100(Fe) with magnetic separation and oxidase-like activities in food samplesAptamerTACTATCGCGGGAGACAGCGCGGGAGGCACCGGGGA36N/AssDNAListeria Monocytogenes (ATCC 15313)Whole cellDeveloped a colorimetric aptasensor based on magnetic responsiveness and oxidase-like activity of Fe3O4@MIL-100(Fe) for the detection of L.monocytogenes.The linear range was from 10(2) to 10(7) CFU/mL, with the limit of detection as low as 14 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1855 388059472024Aptamer-functionalized Fe3O4/MWCNTs@Mo-CDs nanozyme for rapid colorimetric detection toward Escherichia coliE. coli aptamerGCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (ATCC 8099)Whole cellPeroxidase (POD)-like Fe3O4/MWCNTs@Mo-CDs (FMMC) nanozyme was developed for the colorimetric detection of E. coli.Had a wide linear range of 10(1)–10(6) CFU/mL, low LOD of 0.978 CFU/mL.N/AN/AN/ABiosensorN/AFAM LabeledN/AN/AN/AN/AN/A
ABdb_1869 376398932024Nano-biosensor based on manganese dioxide nanosheets and carbon dots for dual-mode determination of Staphylococcus aureusAptamer1TCGGCACGTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTC43N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a fluorescence and colorimetry dual-mode sensor to detect S. aureus by MnO2 NSs and CDs.Had a broad linear range of 37 ∼ 3.7 × 10(6) CFU/mL and low detection limits of 9 CFU/mL (ratiometric fluorescence) and 22 CFU/mL (colorimetry).N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1870 376398932024Nano-biosensor based on manganese dioxide nanosheets and carbon dots for dual-mode determination of Staphylococcus aureusAptamer2GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT45N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a fluorescence and colorimetry dual-mode sensor to detect S. aureus by MnO2 NSs and CDs.Had a broad linear range of 37 ∼ 3.7 × 10(6) CFU/mL and low detection limits of 9 CFU/mL (ratiometric fluorescence) and 22 CFU/mL (colorimetry).N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1871 386698462024Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensorsS. enterica aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-functionalized CNT-FET biosensor for the detection of these foodborne pathogens.Could detect S. enterica at a limit of detection (LOD) as low as 1 CFU in PBS buffer with a linear range from 62.5–6.4 × 10(4) CFU/mL.N/AFlow Cytometry8.9 ± 2.0 nMBiosensorN/A5'-Thiolated (HS-(CH2)6), 5'-FAM Labeled and 3'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1872 386698462024Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensorsS. aureus aptamerAACGAGGCGCAGGGGGAGGGGGTGGTACAGATAAGATGGGG41N/AssDNAStaphylococcus aureus (S. aureus) (CMCC(B) 26003)Whole cellDeveloped an aptamer-functionalized CNT-FET biosensor for the detection of these foodborne pathogens.Could detect S. aureus at a limit of detection (LOD) as low as 1.2 CFU in PBS buffer with a linear range from 10(2)-1.28 × 10(4) CFU/mL.N/AFlow Cytometry0.65 ± 0.2 μMBiosensorN/A5'-Thiolated (HS-(CH2)6), 5'-FAM Labeled and 3'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1890 403089702025Aptamer-functionalized graphene quantum dots combined with artificial intelligence detect bacteria for urinary tract infectionsAptamerCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCG45N/AssDNAEscherichia Coli (E. Coli)Whole cellDevelop an aptamer-functionalized graphene quantum dots integrated with an artificial intelligence detection system (AG-AI detection system) for the detection of E. coli.Exhibits a wide linearity (10(3)-10(9) CFU/mL) and a low detection limit (3.38×10(2) CFU/mL).N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1891 408555242025Inhibition of Enterotoxigenic Escherichia coli adhesion via aptamers prevents infection in IPEC-J2 cellsK88-Apt A04GGGAGCTCAGAATAAACGCTCAACCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTGTTCGACATGAGGCCCGGATC78N/AssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)K88 fimbriae proteinAn aptamer was identified to effectively inhibit the adhesion of ETEC K88 to intestinal epithelial cell IPEC-J2 and reduce ETEC K88-induced cytotoxicity in IPEC-J2 cells in vitro.K88-Apt A04 may more effectively displace ETEC K88 and induce less cytotoxicity than K88-Apt 37, as it resulted in lower TNF-α expression.N/AN/A34.51 ± 9.15 nMTherapeuticsN/A5'-FAM LabeledK88-Apt A04 indicated the lowest cytotoxicity (5.18%) at 100 nMN/ABest CandidateN/AN/A
ABdb_1892 408555242025Inhibition of Enterotoxigenic Escherichia coli adhesion via aptamers prevents infection in IPEC-J2 cellsK88-Apt 37GGAGACCGTACCATCTGTTCGTGGAAGCGCTTTGCTCGTCCATTAGCCTTGTGCTCGTGC60N/AssDNAEscherichia Coli (E. Coli) ETEC K88 (CVCC 216)Whole cellAn aptamer was identified to effectively inhibit the adhesion of ETEC K88 to intestinal epithelial cell IPEC-J2 and reduce ETEC K88-induced cytotoxicity in IPEC-J2 cells in vitro.K88-Apt A04 may more effectively displace ETEC K88 and induce less cytotoxicity than K88-Apt 37, as it resulted in lower TNF-α expression.N/AN/A21.68 ± 4.65 nMTherapeuticsN/A5'-FAM LabeledK88-Apt 37 revealed the lowest cytotoxicity (8.77%) at 50 nM.N/AN/AN/AN/A
ABdb_1893 412456392025Simultaneous and sensitive detection of Mycobacterium tuberculosis and SARS-CoV-2 antigens employing an electrochemical impedance spectroscopy aptasensorMPT64 aptamer sequence (17)GTCAACAGTCCAGTTGGGAGGTCGCTTATAGCTGCTTGTC40N/AssDNAMycobacterium Tuberculosis (M.Tb.)MPT64 proteinDevelop an EIS-based aptasensor fabricated to detect MPT64 and S-glycoprotein simultaneously, employing a dual-platform approach on a screen-printed gold electrode (SPGE).Achieved detection limits of 0.053 pg/mL in buffer and in human serum of 0.085 pg/mL with a linear range of 0.01 pg/mL to 10 pg/mL in both buffer and human serum.N/AN/A8.92 nMBiosensorN/A5'-Thiolated (HS(CH2)6-(CH2CH2O)6-TTTTT)N/AThe aptasensor maintained good storage stability for up to 22 days.N/AN/AN/A
ABdb_1894 397933722025Aptamer-molecularly imprinted polymer sensors for the detection of bacteria in waterLPS AptamerTAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCA45N/AssDNAEscherichia Coli (E. Coli) DH5αLipopolysaccharide (LPS)Developed an electrochemical sensor based on aptamer-molecularly imprinted polymer (Apta-MIP) for the multiplexed detection of Staphylococcus aureus and Escherichia coli.Can detect E. coli with a limit of detection of 2 CFU/mL and exhibited a broad dynamic range from 1 CFU/mL to 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1895 397933722025Aptamer-molecularly imprinted polymer sensors for the detection of bacteria in waterS. aureus aptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) strain SH1000Whole cellDeveloped an electrochemical sensor based on aptamer-molecularly imprinted polymer (Apta-MIP) for the multiplexed detection of Staphylococcus aureus and Escherichia coli.Can detect S. aureus with a limit of detection of 4 CFU/mL and exhibited a broad dynamic range from 1 CFU/mL to 10(8) CFU/mL.N/AN/AN/ABiosensorN/A5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1903 413395962025Self-reduced Pd nanoparticles on NiMn-LDHs with superior catalytic activity for signal amplification in electrochemical aptasensing of Mycobacterium tuberculosis ESAT-6 antigenAptGGGAGCTCAGAATAAACGCTCAACCTCCCTGGGTCACCATAGACTCCATCTAAGATGCTTCGACATGAGGCCCGGATC78N/AssDNAMycobacterium Tuberculosis (M.Tb.)ESAT-6 (6-kDa early secretory antigenic target)Developed an electrochemical aptasensor using NiMn-layered double hydroxides@palladium nanoparticles (NiMn-LDHs@PdNPs) for detecting the ESAT-6 antigen in human serum.The aptasensor exhibited a wide linear detection range of 75 pg/mL to 10 ng/mL with a low limit of detection (LOD) of 0.629 pg/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂-(CH2)6) and 5'-BiotinylatedN/AAptasensor was stored at 4°C for and after 30 days, the current remained approximately 80% of the initial value.N/AN/AN/A
ABdb_1904 405175692025Ultrasensitive electrochemical aptasensor for Pseudomonas aeruginosa detection using N-doped MWCNTs/AgNPs nanocompositeF23CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellDeveloped an electrochemical P.A. biosensor based on N-MWCNTs/AgNPs-10/Apt for the detection of P. aeruginosa.Exhibited a wide linear detection range from 10(-1) to 10(6) CFU/mL, and the limit of detection is 0.0798 CFU/mL.N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1905 411236782025Portable AI-driven electrochemical aptasensor for real-time Staphylococcus aureus detection in food and beveragesIsdA-specific aptamerGCGCACGCGUGUGUAGUACACACGAUCGCGCGCACAAUAU40N/AssRNAStaphylococcus aureus (S. aureus) (ATCC 25923)Iron-regulated Surface Determinant Protein A (IsdA)Developed a portable electrochemical aptasensor integrated with machine learning using SPCE/AuNPs for the detection of S. aureus in food and beverage samples.The lowest detection limit for S. aureus was 1 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1906 409479252025Smartphone-Based Fluorescence/Colorimetric Dual-Mode Aptasensor for the Detection of Salmonella Using Multivalent Aptamer and CHA AmplificationAptamerGTGAGAGTGGGTCATCGAAAAAACTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTCTGATGTCGGTAGT82N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped a dual-mode portable fluorescent/colorimetric aptasensor, leveraging multivalent aptamer competitive assays and catalytic hairpin assembly (CHA) for the detection of Salmonella.Enables the quantitative detection of Salmonella within a linear range of 10 to 10(7) CFU/mL, with detection limits of 28 CFU/mL for colorimetric detection and 10 CFU/mL for fluorescent detection.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1907 411492872025Real-Time and Selective Detection of Pseudomonas aeruginosa in Beef Samples Using a g-C3N4-Doped Multimetallic Perovskite-Based Electrochemical AptasensorP. aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas AeruginosaWhole cellDeveloped an electrochemical aptasensor based on aptamer functionalized FeCoCuNiO-g-C3N4 nanocomposite for the detection of P. aeruginosa in food samples.Exhibited a low detection limit of 3.03 CFU/mL and over a range of 1 × 10(1)–1 × 10(7) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1908 408017762025Ultrasensitive Aptamer-Based Metal-Organic Framework-on-Metal-Organic Framework Platform for Clinical Detection of KPC-2 Klebsiella pneumoniae and Multidrug-Resistant Acinetobacter baumanniiKPC-2 KP aptamerGGCAGGACACCGTAACGGGTATGCAGCTATCCCGGGCGCTGTCTGAAGATCGTGTGCTGC60N/AssDNAKlebsiella Pneumoniae carbapenemase 2-expressing K. Pneumoniae (KPC-2 KP)Klebsiella Pneumoniae Carbapenemase 2 (KPC-2) serine β-lactamaseDeveloped a dual nanozyme-powered colorimetric aptasensor leveraging a cascade amplification mechanism, a metal-organic framework (MOF)-on-MOF nanostructure with peroxidase-like activity for detection of KPC-2 K. pneumoniae and MDR-AB.The system achieves selective bacterial capture within 40 min, quantifying 10–10(8) CFU/mL with a detection limit of 7 CFU/mL for KPC-2 KP.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1909 408017762025Ultrasensitive Aptamer-Based Metal-Organic Framework-on-Metal-Organic Framework Platform for Clinical Detection of KPC-2 Klebsiella pneumoniae and Multidrug-Resistant Acinetobacter baumanniiMDR-AB aptamerCAGGGGACGCACCAAGGTTTTGTTTTTTCTTTGCTTCTTTTTGCTTTTTTTTCCATGACCCGCGTGCTGCGTGA74N/AssDNAMultidrug-resistant (MDR) Acinetobacter BaumanniiWhole cellDeveloped a dual nanozyme-powered colorimetric aptasensor leveraging a cascade amplification mechanism, a metal-organic framework (MOF)-on-MOF nanostructure with peroxidase-like activity for detection of KPC-2 K. pneumoniae and MDR-AB.The system achieves selective bacterial capture within 40 min, quantifying 10–10(8) CFU/mL with a detection limit of 6 CFU/mL for MDR-AB.N/AN/AN/ABiosensorN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1910 409313012025A novel fluorescent aptasensor using magnetic silica nanospheres and Ag@MOF nanocomposites for extraction and detection of Salmonella Typhimurium in eggsAptamerTATGGCGGCGTCACCCGACGGGGACTTGCATTATGACAG39N/AssDNASalmonella Typhimurium (S. Typhimurium) (PTCC 1709)Whole cellDeveloped a fluorescent platform based on an aptamer-Ag@MOF and Fe3O4@KCC‐1 probes for the detection of S. Typhimurium in eggs.Achieved a linear detection range of 7.5 to 7.5 × 10⁶ CFU/mL, a detection limit of 4.61 CFU/mL, and a total detection time of 90 min.N/AN/AN/ABiosensorN/A3'-ThiolatedN/AN/AN/AN/AN/A
ABdb_1911 408390542025SERS-based aptasensor for culture-free detection of Escherichia coli in urinary tract infection diagnosisAptamerGCAATGGTACGGTACTTCCCCATGAGTGTTGTGAAATGTTGGGACACTAGGTGGCATAGAGCCGCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellDeveloped a SERS-based aptasensor for the detection of E. coli.Demonstrated a detection limit of 5.9 × 10(3) CFU/mL, which is well below the UTI cutoff value.N/AN/AN/ABiosensorN/A3'-BHQ2N/AN/AN/AN/AN/A
ABdb_1912 404372832025Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO2 nanosheets system for Escherichia coli detectionAwIATCAAATGTGCAGATATCAAGACGATTTGTACAAGATCCATGCTGAGGTGGTCATAGCTGATCCTACCTGTACAAATCGTCTTGAT86N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellDeveloped a label-free fluorescent detection system leveraging target-triggered hybridization chain reaction (HCR) amplification for E. coli detection.Exhibited a detection limit of 17 CFU/mL and a broad dynamic range from 5.0 × 10(1) ~ 5.0 × 10(7) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1913 4018787020253D DNA walkers integrated with self-reporting MOFs: Pioneering ratiometric electrochemical sensing for Staphylococcus aureusS. aureus aptamer (Apt)GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a ratiometric electrochemical aptasensor utilizing a magnetic 3D DNA walking machine and self-assembly of MOF for the sensitive detection of S. aureus.Demonstrates a detection range from 10 to 2 × 10(5) CFU/mL and achieves a low LOD of 2.3 CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AAfter a duration of seven days, the ratio value maintained 91.89% of its initial intensity.N/AN/AN/A
ABdb_1914 403357842025A novel strategy for ultrasensitive detection and effective inactivation of Staphylococcus aureus based on Fe3O4-QCS-PEI-Cu-aptamer and ladder-branch HCRAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped an integrated strategy based on Fe3O4-quaternary ammonium chitosan-polyetherimide-Cu-aptamer (Fe3O4-QCS-PEI-Cu-aptamer) and ladder-branch hybridization chain reaction (HCR) for the detection and inactivation of S. aureus.The sensor demonstrated a limit of detection (LOD) of 3 CFU/mL for S. aureus and, under near-infrared (NIR) irradiation, achieved an antimicrobial efficiency of 99.946%.N/AN/AN/ADiagnostic/TherapeuticsN/A3'-COOH (Carboxylated)N/AN/AN/AN/AN/A
ABdb_1926 401369432025Nanoparticle-Enhanced Acoustic Wave Biosensor Detection of Pseudomonas aeruginosa in FoodP. aeruginosa aptamerCCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa PAO1Whole cellDeveloped a biosensor for detecting P. aeruginosa in whole milk samples using an aptamer functionalized antifouling linking molecule 3-(2-mercaptoethanoxy)propanoic acid (HS-MEG-COOH), and another one with the addition of AuNPs.Exhibited a linear range 10(2)-10(5) CFU/mL and limit of detection (LOD) of 86 CFU/mL in PBS and 157 CFU/mL in milk, and with AuNPs, reducing the extrapolated LOD to 68 CFU/mL in PBS and 46 CFU/mL in milk.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1927 394060882025Laser-induced graphene-based aptasensor for the selective detection of Escherichia coli in urine samplesP12-55CATACGATTTAGGTGACACTATAGCCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGAATTTCTCCTACTGGGATAGGTGGA88N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellDeveloped an impedance biosensor using a Laser-Induced Graphene-based (LIG) electrode for the detection of E. coli in urine samples.The aptasensor showed a linear response to E. coli over the range 10(0)-10(3) CFU/mL and LOD of 9 CFU/mL in PBS.N/AN/AN/ABiosensorN/A3'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1928 409166372025Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary ElectrophoresisAP1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellDeveloped an aptamer-based capillary sieving electrophoresis (CE)-Laser-induced fluorescence (LIF) detection of three bacteria, Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus.The measured limit of detection (LOD) for V. parahaemolyticus was 3.70 × 10(6) CFU/mL and a linear range of 0.80-124 × 10(7) CFU/mL.N/AN/AN/ADetectionN/A5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1929 409166372025Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary ElectrophoresisAP2CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellDeveloped an aptamer-based capillary sieving electrophoresis (CE)-Laser-induced fluorescence (LIF) detection of three bacteria, Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus.The measured limit of detection (LOD) for E. coli was 1.28 × 10(7) CFU/mL and a linear range of 6.50 - 153 × 10(7) CFU/mL.N/AN/AN/ADetectionN/AExtension chain for AP2 (T2) , 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1930 409166372025Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary ElectrophoresisAP3TCCCTACGGCGCTAACCTCCCAACCGCTCCACCCTGCCTCCGCCTCGCCACCGTGCTACAAC62N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 6538)Whole cellDeveloped an aptamer-based capillary sieving electrophoresis (CE)-Laser-induced fluorescence (LIF) detection of three bacteria, Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus.The measured limit of detection (LOD) for S. aureus was 1.67 × 10(7) CFU/mL and a linear range of 3.00-150 × 10(7) CFU/mL.N/AN/AN/ADetectionN/AExtension chain for AP3 (T3), 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1931 399618262025Self-protective DNAzyme-based dual-responsive three-way Y-probe for simultaneous determination of multiple pathogenic bacteriaAPT(S)CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT59N/AssDNASalmonella Typhimurium (S. Typhimurium) (KCTC 1925)Whole cellDeveloped a DNAzyme-based self-protecting dual-response three-way Y nanoprobe (SD-DTY) for the simultaneous detection of two foodborne pathogens.The limit of detection was 3.2 cfu/mL, and the linear range was 10-10(5) cfu/mL, with a 2-hour response time.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1932 399618262025Self-protective DNAzyme-based dual-responsive three-way Y-probe for simultaneous determination of multiple pathogenic bacteriaAPT(E)CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACACCTACCCATCGGA54N/AssDNAEscherichia Coli (E. Coli) O157:H7 (KCTC 2571)Whole cellDeveloped a DNAzyme-based self-protecting dual-response three-way Y nanoprobe (SD-DTY) for the simultaneous detection of two foodborne pathogens.The limit of detection was 3.7 cfu/mL, and the linear range was 10-10(5) cfu/mL, with a 2-hour response time.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1933 https://doi.org/10.1016/j.snb.2024.1366092025Ultrasensitive electrochemical biosensor for bacteria detection based on Fe3O4@COF-AuNPs and trigging isothermal circular amplificationAptamerCCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG42N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped an ultrasensitive electrochemical biosensor based on magnetic nanocomposite material Fe3O4@COF-AuNPs and triggering isothermal circular amplification (TICA) for E. coli detection.The detection limit (LOD) was 10 CFU/mL, with a linear range of 10(2)−10(9) CFU/mL, and the detection time was 1 h.N/AN/AN/ABiosensorN/AN/AN/AThe sensor was stored at 4°C, and the signal value decreased by only 6.28 % over 30 days.N/AN/AN/A
ABdb_1945 413440102026SERS-based Aptasensor using Au NSs@Ag NPs/magnetic graphene oxide assemblies for simultaneous detection of Escherichia coli and Staphylococcus aureusApt1 & Apt3ATCCGTCACACCTGCTCTACTGGCCGGCTCAGCATGACTAAGAAGGAAGTTATGTGGTGTTGGCTCCCGTATTTTTTTTTT81N/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped a SERS aptasensor based on aptamer-magnetic graphene oxide (MGO) and dual-functional Au NSs@Ag NPs probes for simultaneous detection of E. coli and S. aureus.The SERS intensity shows a good linear relationship over a range of 50 to 10(6) CFU/mL, with a detection limit of 16 CFU/mL for E. coli.N/AN/AN/ABiosensorN/A5'-Thiolated (for apt1 & apt2) and 5'-Amidation (NH₂) (for apt3 & apt4)N/AN/AN/AN/AN/A
ABdb_1946 413440102026SERS-based Aptasensor using Au NSs@Ag NPs/magnetic graphene oxide assemblies for simultaneous detection of Escherichia coli and Staphylococcus aureusApt2 & Apt4TCCCTACGGCGCTAACCCCCCCAGTCCGTCCTCCCAGCCTCACACCGCCACCGTGCTACAACTTTTTTTTT71N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SERS aptasensor based on aptamer-magnetic graphene oxide (MGO) and dual-functional Au NSs@Ag NPs probes for simultaneous detection of E. coli and S. aureus.The SERS intensity shows a good linear relationship over a range of 50 to 10(6) CFU/mL, with a detection limit of 20 CFU/mL for S. aureus.N/AN/AN/ABiosensorN/A5'-Thiolated (for apt1 & apt2) and 5'-Amidation (NH₂) (for apt3 & apt4)N/AN/AN/AN/AN/A
ABdb_1947 412897762026Improving electrochemical aptasensor sensitivity for Bacillus cereus spore detection in food safety applicationsApt1CATCCGTCACACCTGCTCGGTGCAGACCCATAGGGGGGGCGTGCGGATGTGCGGAGTAGGGTGTTCGCTCCCGTATC77N/AssDNABacillus Cereus strain INRA-SV-S51B. cereus sporesDeveloped an electrochemical aptasensor for the detection of B. cereus spores.An aptasensor based on three aptamers exhibited a wide dynamic range (10(2)–10(7) CFU/mL) and a low limit of detection (∼1 CFU/mL) using only 15 μL of sample.N/AN/AN/ABiosensorN/A5′-HO(CH2)6SS(CH2)6(T)5 group, or 5'-TexasRed LabeledN/AN/AN/AN/AN/A
ABdb_1948 412897762026Improving electrochemical aptasensor sensitivity for Bacillus cereus spore detection in food safety applicationsApt2CATCCGTCACACCTGCTCCCCAATGAAGCGAGATGGACGCCTAGCACCCCCCGCGTCCGGTGTTGGCTCCCGTATC76N/AssDNABacillus Cereus strain INRA-SV-S51B. cereus sporesDeveloped an electrochemical aptasensor for the detection of B. cereus spores.An aptasensor based on three aptamers exhibited a wide dynamic range (10(2)–10(7) CFU/mL) and a low limit of detection (∼1 CFU/mL) using only 15 μL of sample.N/AN/AN/ABiosensorN/A5′-HO(CH2)6SS(CH2)6(T)5 group, or 5'-TexasRed LabeledN/AN/AN/AN/AN/A
ABdb_1949 412897762026Improving electrochemical aptasensor sensitivity for Bacillus cereus spore detection in food safety applicationsBAS6ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGATGGTGTTGGCTCCCGTAT71N/AssDNABacillus Cereus strain INRA-SV-S51B. cereus sporesDeveloped an electrochemical aptasensor for the detection of B. cereus spores.An aptasensor based on three aptamers exhibited a wide dynamic range (10(2)–10(7) CFU/mL) and a low limit of detection (∼1 CFU/mL) using only 15 μL of sample.N/AN/AN/ABiosensorN/A5′-HO(CH2)6SS(CH2)6(T)5 group, or 5'-TexasRed LabeledN/AN/AN/AN/AN/A
ABdb_1950 411351622026Silver-decorated nanobeads for high-performance and flexible analysis of Shigella dysenteriae utilizing a paper-based aptasensorAptamerATAGGAGTCACGACGACCAGAACGGAACTAGCGTTTAAATGCCAGGACTGAAGTAGCAGGGATTAGTCAAGAGGTAGACGCACATA86N/AssDNAShigella DysenteriaeWhole cellDeveloped an Ag/MBs-modified paper-based aptasensor to detect S. dysenteriae.MB-based assays achieve broad linearity (DPV = 10(2)–10(8) CFU/mL, EIS = 10(1)–10(9) CFU/mL) and high sensitivity (DPV = 90 CFU/mL, EIS = 8.09 CFU/mL).N/AN/AN/ABiosensorN/A5'-Amidation (NH₂)N/ALong stability of up to 28 days at 4°C.N/AN/AN/A
ABdb_1970 233816902013Selection of aptamers against inactive Vibrio alginolyticus and application in a qualitative detection assayPool of enriched aptamersN/AN/A5'-TCAGTCGCTTCGCCGTCTCCTTC-N35-GCACAAGAGGGAGACCCCAGAGGG-3'ssDNAVibrio AlginolyticusWhole cell (Inactivated)Identify aptamers against and qualitatively detect inactive Vibrio alginolyticus using PCR.V. alginolyticus could be detected at 100 cells/ml.15Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)27.5 ± 9.2 nMDetectionN/AN/AN/AN/AN/AN/AN/A
ABdb_2009 https://doi.org/10.1016/j.lwt.2013.12.0122014Development and evaluation of aptamer magnetic capture assay in conjunction with real-time PCR for detection of Campylobacter jejuniAptamer 229N/AN/AN/AssDNACampylobacter Jejuni (A9a)Whole cellDevelop an aptamer-based magnetic capture-qPCR (AMC-qPCR) assay for the detection of Campylobacter jejuni.Capture efficiency was 10–13% at the detection limit of 1.1 log10/300 μl C. jejuni cells and a range of 1.0–2.0 log10 CFU per sample (0.3-10 ml).N/AN/AN/ADetectionN/A5'-BiotinylatedN/AN/AN/AN/AWO Patent WO/2011/097420
ABdb_2023 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications4948-1N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85A (Rv3804c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.05 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2024 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications4953-64N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85A (Rv3804c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.06 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2025 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12073-8N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85A (Rv3804c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.09 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2026 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12073-32N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85A (Rv3804c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.16 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2027 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12092-7N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85A (Rv3804c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.06 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2028 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14492-7N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85A (Rv3804c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.24 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2029 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14492-11N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85A (Rv3804c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.21 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2030 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14504-6N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85A (Rv3804c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.29 pM, 0.97 pM, 0.92 pM and 7.3 × 10(6) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.07 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2031 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications4949-52N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85B (Rv1886c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay6.39 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2032 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications4954-5N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85B (Rv1886c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.31 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2033 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12074-5N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85B (Rv1886c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.27 pM, 0.18 pM, 0.30 pM and 5.5 × 10(5) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.08 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2034 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12074-11N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85B (Rv1886c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.36 pM, 0.72 pM, 0.18 pM and 1.8 × 10(6) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.14 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2035 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12093-26N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85B (Rv1886c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.26 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2036 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14493-5N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85B (Rv1886c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay1.94 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2037 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14493-16N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85B (Rv1886c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.29 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2038 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14505-57N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85B (Rv1886c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 4.15 pM, 5.13 pM, 55.47 pM and 4 × 10(7) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.13 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2039 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications4950-27N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.36 pM, 1.80 pM, 0.61 pM and 3.4 × 10(8) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.03 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2040 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications4955-49N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.26 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2041 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications5569-2N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.23 pM, 0.28 pM, 0.36 pM and 2.6 × 10(7) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.01 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2042 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications5575-1N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.03 nMBiosensorN/APEdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2043 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12075-16N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 7.39 pM, 5.38 pM, 8.51 pM and 4.9 × 10(8) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.05 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2044 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12075-40N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.13 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2045 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14494-53N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.03 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2046 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14494-124N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.14 pM, 0.07 pM, 0.06 pM and 2.7 × 10(7) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.02 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2047 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14506-48N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.89 pM, 1.98 pM, 2.10 pM and 3.3 × 10(8) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.04 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2048 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14506-76N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)A85C (Rv0129c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.03 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2049 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7604-59N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)ACR (Rv2031c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay17.5 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2050 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7616-43N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)ACR (Rv2031c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay20.3 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2051 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14483-8N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)ACR (Rv2031c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay32 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2052 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7610-49N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CF30 (Rv0577 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay5.41 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2053 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7618-50N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CF30 (Rv0577 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay2.2 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2054 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12089-13N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CF30 (Rv0577 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay1.83 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2055 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7595-51N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH10 (Rv3418c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.43 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2056 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7600-67N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH10 (Rv3418c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay9.45 nMBiosensorN/ABndU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2057 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7608-61N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH10 (Rv3418c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay11.1 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2058 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12067-1N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH10 (Rv3418c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay7.55 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2059 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14488-1N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH10 (Rv3418c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.40 pM, 0.84 pM, 1.94 pM and 1.1 × 10(6) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.53 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2060 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14488-3N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH10 (Rv3418c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.05 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2061 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7592-57N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH602 (Rv0440 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.26 pM, 1.47 pM, 1.98 pM and 3.0 × 10(7) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.46 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2062 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7605-11N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH602 (Rv0440 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay1.84 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2063 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14484-4N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH602 (Rv0440 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.34 pM, 0.56 pM, 1.20 pM and 2.2 × 10(8) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.5 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2064 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14484-33N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH602 (Rv0440 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay3.03 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2065 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14498-14N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)CH602 (Rv0440 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay7.39 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2066 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7606-49N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)DNAK (Rv0350 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.59 pM, 1.53 pM, 2.19 pM and 1.6 × 10(7) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.66 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2067 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14486-2N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)DNAK (Rv0350 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.31 pM, 0.78 pM, 3.03 pM and 7.4 × 10(6) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay1.03 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2068 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7612-56N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)ESXA (Rv3875 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay41.4 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2069 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7620-5N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)ESXA (Rv3875 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay54.7 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2070 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications5557-2N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)ESXB (Rv3874 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.12 pM, 0.32 pM, 0.13 pM and 2.2 × 10(6) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.54 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2071 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications5562-95N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)ESXB (Rv3874 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay4.52 nMBiosensorN/APEdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2072 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7598-15N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)KAD (Rv0733 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay1.11 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2073 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12090-3N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)KAD (Rv0733 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay13.2 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2074 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14491-10N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)KAD (Rv0733 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.20 pM, 1.11 pM, 24.55 pM and 7.9 × 10(7) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.08 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2075 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14491-43N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)KAD (Rv0733 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.14 pM, 0.34 pM, 6.19 pM and 3.5 × 10(7) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.12 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2076 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14502-11N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)KAD (Rv0733 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay1.28 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2077 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14502-14N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)KAD (Rv0733 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.83 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2078 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14487-46N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MASZ (Rv1837c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay6.6 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2079 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14499-2N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MASZ (Rv1837c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay8.84 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2080 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14999-10N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MASZ (Rv1837c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay5.27 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2081 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14999-49N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MASZ (Rv1837c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay4.67 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2082 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15005-35N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MASZ (Rv1837c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay4.61 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2083 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15005-42N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MASZ (Rv1837c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay4.08 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2084 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15013-1N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MASZ (Rv1837c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay8.06 nMBiosensorN/ABndU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2085 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15013-72N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MASZ (Rv1837c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay8.54 nMBiosensorN/ABndU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2086 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7615-18N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MPT64 protein (Rv1980c)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay12.3 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2087 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14496-43N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MPT64 protein (Rv1980c)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay2.07 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2088 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications5560-59N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MPT51 protein (Rv3803c)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay13.9 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2089 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7619-25N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MPT51 protein (Rv3803c)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay11.1 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2090 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14503-5N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MPT51 protein (Rv3803c)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay21.1 nMBiosensorN/APPdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2091 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15001-2N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MTB12 (Rv2376c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.18 pM, 0.11 pM, 0.42 pM and 1.1 × 10(6) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.04 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2092 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15001-29N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MTB12 (Rv2376c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.06 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2093 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15001-182N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MTB12 (Rv2376c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.18 pM, 1.68 pM, 0.93 pM and 3.4 × 10(5) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.05 nMBiosensorN/ANapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2094 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15007-1N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MTB12 (Rv2376c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.24 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2095 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15007-43N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MTB12 (Rv2376c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.18 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2096 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications15007-48N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)MTB12 (Rv2376c gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.18 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2097 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications5558-86N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)PSTS1 (Rv0934 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay11.5 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2098 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7622-15N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)PSTS1 (Rv0934 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay6.68 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2099 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14485-44N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)PSTS1 (Rv0934 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay5.75 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2100 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14485-59N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)PSTS1 (Rv0934 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay9.1 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2101 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7587-49N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)RL7 (Rv0652 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.26 pM, 2.47 pM, 1.05 pM and 1.2 × 10(8) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.07 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2102 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7596-2N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)RL7 (Rv0652 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.Exhibit LOD of 0.08 pM, 0.70 pM, 1.35 pM and 3.4 × 10(7) cells/ml in Buffer, Normal serum(40%), Urine protein (100 μg) and serum bacterial load, respectively.N/ARadiolabel Equilibration Binding Assay0.13 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/ABest CandidateN/AN/A
ABdb_2103 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications12087-24N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)RL7 (Rv0652 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay11.1 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2104 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14489-14N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)RL7 (Rv0652 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.11 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2105 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14489-18N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)RL7 (Rv0652 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.04 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2106 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications7609-13N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)TPX (Rv1932 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay3.56 nMBiosensorN/ATrpdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2107 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14490-6N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)TPX (Rv1932 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay0.72 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2108 287941782017Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications14490-127N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (NR-14865; BEI Resources)TPX (Rv1932 gene)Slow-off-rate modified aptamer (SOMAmer) for detecting TB.N/AN/ARadiolabel Equilibration Binding Assay1.5 nMBiosensorN/A2NapdU and 5′PBDC (photocleavable biotin, D spacer, Cyanine3 (Cy3) Labeled) and 3’-Inverted Thymidine (3'-idT)N/AN/AN/AN/AN/A
ABdb_2117 283421482017Further characterization and independent validation of a DNA aptamer-quantum dot-based magnetic sandwich assay for CampylobacterAptamerN/AN/AN/AssDNACampylobacter Jejuni (ATCC 29428)Whole cellDeveloped an aptamer-MB and aptamer-QD sandwich assay for the detection of C.jejuni.Establishes a LOD between 5 and 10 C. jejuni cells/mL in sterile buffer (PBS) and chicken rinsate.N/AN/AN/ABiosensorN/A5'-BiotinylatedN/AN/AN/AN/AU.S. Patent No. 9562900
ABdb_2118 303824042018A bimodal (SERS and colorimetric) aptasensor for the detection of Pseudomonas aeruginosaP. aeruginosa aptamerN/AN/AN/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellThe dual-mode aptasensor based on SERS and colorimetry assay for the determination of P. aeruginosa.LOD of the aptasensor was 20 cfu/mL for SERS mode and 50 cfu·mL−1 for color mode with a linear range from 10^2 cfu/mL to 10^6 cfu/mL.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_2120 https://doi.org/10.1016/j.jelechem.2018.07.0022018Target-induced aptamer displacement on gold nanoparticles and rolling circle amplification for ultrasensitive live Salmonella typhimurium electrochemical biosensingS. typhimurium (STM)-binding aptamer.N/AN/AN/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDevelop an aptasensor for ultrasensitive detection of live S. typhimurium by combining target-induced aptamer displacement on gold nanoparticles (AuNPs) deposited electrode with rolling circle amplification (RCA).Showed a LOD of 16 CFU/mL with a wide linear detection range of 20 to 2 × 10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AWhen the sensor was stored at 4°C for 10 days, the electrical signal response was 90% of that obtained at the time of preparation.N/AN/AN/A
ABdb_2121 317001252019An aptasensor for the detection of Mycobacterium tuberculosis secreted immunogenic protein MPT64 in clinical samples towards tuberculosis detectionAptamerN/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv)MPT64 proteinElectrochemical impedance spectroscopy (EIS) aptasenosor based on an IDE for the detection of MPT64.Display LOD of 4.1 fM and the specificity and sensitivity for the sputum sample analysis 100% and 76.47%, respectively, and for the serum sample analysis 100% and 88.24%, respectively.N/AN/A8.92 nMBiosensorN/A5'-Thiolated (HS-(CH6)6-OP(O)2O-(CH2CH2O)6-5′-TTTTT)N/AN/AN/AN/AN/A
ABdb_2122 333674182021Photo-responsive functional gold nanocapsules for inactivation of community-acquired, highly virulent, multidrug-resistant MRSAAptamerN/AN/A5'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA) “superbug” (USA300)Whole cellAptamers functionalization of Cur@mPEG-SH@GNRs for PTT and ROS.Blocked biofilm formation and killed all of the trapped bacteria in 30 min during NIR stimulation.N/AN/AN/ATargeted Delivery/TherapeuticsN/A5'-ThiolatedN/AN/AN/AN/AN/A
ABdb_2123 333955712021Aptasensor for the detection of Methicillin resistant Staphylococcus aureus on contaminated surfacesAT0033N/AN/AN/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA) (ATCC 25923)Penicillin binding protein 2a (PBP2a)Developed a colorimetric aptasensor for the detection of MRSA on contaminated surfaces.The visual detection limit was less than 100 CFU/ml and, theoretically, 2 CFU/ml, while the linear range of quantitation was 10(2)–10(5) CFU/ml.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_2129 357654982022Single-stranded DNA aptamer-based rolling circle amplification as anti-chicken Salmonella bacteriostaticAnti-Salmonella DNA aptamerN/AN/AN/AssDNASalmonella Typhimurium (S. Typhimurium) and Salmonella Enteritidis (S. Enteritidis)Whole cellIdentify an aptamer targeting Salmonella in the form of RCA-p that could inhibit bacterial growth, multiplication, and viability.Significant reduction in bacterial viability.N/AN/AN/ATherapeuticsN/AN/AN/AN/AN/AN/AN/A
ABdb_2130 377734212023Sensitive detection of Escherichia coli in diverse foodstuffs by electrochemical aptasensor based on 2D porphyrin-based COFAptamerN/AN/AN/AssDNAEscherichia Coli (E. Coli)Whole cellDeveloped an electrochemical aptasensor using two-dimensional porphyrin-based covalent organic framework (Tph-TDC-COF) for the detection of E.coli.Demonstrated an extremely low detection limit of 0.17 CFU/mL for E.coli detection within a linear range of 10 to 1 × 10(8) CFU/mL.N/AN/AN/ABiosensorN/AN/AN/AThe aptasensor recovered to 104.3% after 15 days of storage at 4°C in 0.01 M phosphate buffer, indicating excellent stability.N/AN/AN/A
ABdb_2131 https://doi.org/10.1016/j.bioana.2024.05.0042024Aptamer-carbon quantum dots and silver nanoparticles construct a FRET sensor for sensitive detection of E. coliE. coli aptamerN/AN/AN/AssDNAEscherichia Coli (E. Coli) BL21 strainWhole cellDeveloped a FRET-based fluorescent biosensor by combining CQDs with Ag NPs for the rapid detection of E. coli (BL21).The sensor has a linear range of 2×10(3) ∼ to 2×10(8) CFU/mL and a low detection limit of 77 CFU/mL for E. coli.N/AN/AN/ABiosensorN/AN/AN/AN/AN/AN/AN/A