Method : FluMag (NanoMag)-SELEX

Total records found: 10

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0263 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA1AGCAGCACAGAGGTCAGATG-AGGCGATTACGCTTCTTGTACTTCAATAACGACTCAACTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy92.17 ± 16.75 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0264 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA15/40AGCAGCACAGAGGTCAGATG-TACTTATGCATTTCCTCCCACGATCTTATTTGAGAGTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.Limit of detection of 8.7±10(-3) μg/mL with linear range of 0.01-10 μg/mL SEA.12Fluorescence Spectroscopy48.57 ± 6.52 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0265 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.2AGCAGCACAGAGGTCAGATG-ATGATCGTAGTCATTTAAAATTTGAATACTATCAAAGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence Spectroscopy235.00 ± 79.05 nMBiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0266 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA3AGCAGCACAGAGGTCAGATG-TACGAGCGGTGGTTTTACCCTGCAATACTTTTGGCTGTTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0267 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA25AGCAGCACAGAGGTCAGATG-ATAGATTTTTTTCATGATTCTTCATTTTTTTATTTGAAAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0268 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA29AGCAGCACAGAGGTCAGATG-TATTATCTTACTACGTTGCATCCTACTTTTATAGTCCCCT-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0269 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA30AGCAGCACAGAGGTCAGATG-GGATCTTCCCTCAATGTTTATTGTATATCTGTACTCGTAA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0270 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA10AGCAGCACAGAGGTCAGATG-TCCAATTCAATTCATTTCTGAACTTAGTCGGCACTTTGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0271 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA4AGCAGCACAGAGGTCAGATG-CTCCAGCAGCCTCATAGATACGTCGTATCTATTGTTTCCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0272 https:doi.org10.1039C3AY41576G2013Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin AA23.1AGCAGCACAGAGGTCAGATG-AAGGTCTTACATCAATTTATATATTTTTCCAATATCTGTC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and develop a fluorescent bioassay to detect SEA in the food sample.N/A12Fluorescence SpectroscopyN/ABiosensorFluMag (NanoMag)-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A