Method : Centrifugation-based Partitioning Method

Total records found: 10

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_1357 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20–5GCAATGGTACGGTACTTCC-ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)3.9 ± 0.6 nMDetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1358 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20–7GCAATGGTACGGTACTTCC-CGCAATACCAAAGTGGCGAGAGCGCTGTCTTGAGTGAGTGGTTGG-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)8 ± 0.9 nMDetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1359 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20–10GCAATGGTACGGTACTTCC-TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)10.1 ± 1.7 nMDetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1360 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-1GCAATGGTACGGTACTTCC-GATTAGCTACATTTGGTTGTTTACCGCTCTGCTTTCTATTATTT-CAAAAGTGCACGCTACTTTGCTAA875'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1361 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-2GCAATGGTACGGTACTTCC-TGTTAGTGTTTAAGGCCCAAAGTCGGTTCATCAGTACATTCCTCG-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1362 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-3GCAATGGTACGGTACTTCC-TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA-CAAAAGTGCACGCTACTTTGCTAA855'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1363 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-4GCAATGGTACGGTACTTCC-GATTGTGGTGGGGCCTCGAGATACCTGCGACCGGCATACTTGAAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1364 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-6GCAATGGTACGGTACTTCC-TTGCCCGTACACTGTCATCCTCGGCTTATAGCCATTATTGAAATT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1365 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-8GCAATGGTACGGTACTTCC-GGGCCTATACAGGCTTTTACTTCTGAGTTTGGTAGTTTCTTCGGA-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1366 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-9GCAATGGTACGGTACTTCC-GCGAGGGCCAACGGTGGTTACGTCGCTACGGCGCTACTGGTTGAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A