Method : Biolayer Interferometry based-SELEX (BLI-SELEX)

Total records found: 5

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_1470	32814208	2020	Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensor	Apt(Stx1.1)	ATCCAGAGTGACGCAGCA-GTAGTTTGTTGGTTATTACGGCGGGTTGCGATGGGTGCGAATCGG-TGGACACGGTGGCTTAGT	81	5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'	ssDNA	Escherichia Coli (E. Coli) O157:H7 str. Sakai	Shiga toxin subtype stx1 (Stx1.1)	Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.	LOD of 44.5 pg/mL for stx1 with minimal cross-reactivity and a dynamic response range from 50 pg/mL to 100 ng/mL.	7	Bio-Layer Interferometry (BLI)	8.27 nM (of peptide) and 47.2 pM (of protein)	Detection	Biolayer Interferometry based-SELEX (BLI-SELEX)	N/A	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1471	32814208	2020	Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensor	Apt(Stx1.3)	ATCCAGAGTGACGCAGCA-GGATAGGACGTCAAATTAGGGCCCGGTACAACGAAAGCCCACAAC-TGGACACGGTGGCTTAGT	81	5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'	ssDNA	Escherichia Coli (E. Coli) O157:H7 str. Sakai	Shiga toxin subtype stx1 (Stx1.2)	Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.	N/A	7	Bio-Layer Interferometry (BLI)	31.0 nM (of peptide) and 30.3 μΜ (of protein)	Detection	Biolayer Interferometry based-SELEX (BLI-SELEX)	N/A	N/A	N/A	N/A	N/A	N/A
ABdb_1472	32814208	2020	Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensor	Apt(Stx2.1)	ATCCAGAGTGACGCAGCA-GGGGGCAGGTTCATGGCTTGGGTGCGGTGGGCATGATTCGTGGTG-TGGACACGGTGGCTTAGT	81	5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'	ssDNA	Escherichia Coli (E. Coli) O157:H7 str. Sakai	Shiga toxin subtype stx2 (Stx2.1)	Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.	N/A	7	Bio-Layer Interferometry (BLI)	45.2 nM (of peptide) and 83.8 nM (of protein)	Detection	Biolayer Interferometry based-SELEX (BLI-SELEX)	N/A	N/A	N/A	N/A	N/A	N/A
ABdb_1473	32814208	2020	Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensor	Apt(Stx2.2)	ATCCAGAGTGACGCAGCA-TGTATCTCTTACTTAAGCCTTTGGTTCGGTAACAGCCTGGCATGC-TGGACACGGTGGCTTAGT	81	5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'	ssDNA	Escherichia Coli (E. Coli) O157:H7 str. Sakai	Shiga toxin subtype stx2 (Stx2.2)	Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.	N/A	7	Bio-Layer Interferometry (BLI)	110 nM (of peptide) and 25.2 μM (of protein)	Detection	Biolayer Interferometry based-SELEX (BLI-SELEX)	N/A	N/A	N/A	N/A	N/A	N/A
ABdb_1474	32814208	2020	Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensor	Apt(Stx2.3)	ATCCAGAGTGACGCAGCA-GGAAAGGACGTCAAATTAGGGGCCGGGACAACGAAAGCCCACAAC-TGGACACGGTGGCTTAGT	81	5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'	ssDNA	Escherichia Coli (E. Coli) O157:H7 str. Sakai	Shiga toxin subtype stx2 (Stx2.3)	Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.	LOD of 41.3 pg/mL for stx2 with minimal cross-reactivity and a dynamic response range from 50 pg/mL to 100 ng/mL.	7	Bio-Layer Interferometry (BLI)	4.6 nM (of peptide) and 28.6 pM (of protein)	Detection	Biolayer Interferometry based-SELEX (BLI-SELEX)	N/A	N/A	N/A	Best Candidate	N/A	N/A