Length : 131 - 140

Total records found: 10

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_0009	15023071	2004	In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA	F1-1	GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-ACUGUCCUCCCUUCAGAGAGCGCGGGACCCUUAACUUGGGGCCCACGAACAGCUUCAGUUCCGUCUCGGCGU-CAUAUGUGCGUCUACAUGGAUCCUCA	140	5'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'	ssRNA	Escherichia Coli (E. Coli)	C-terminal ribonuclease domain of Colicin E3	Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.	Addition of aptamer F1-1 in a 1000-fold excess over the colicin E3 CRD completely restored protein synthesis.	12	Nitrocellulose Filter Binding Assay	2 ± 11 nM	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0010	15023071	2004	In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA	F2-1	GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-UCCCUGGCCCAAGAUCCUAAUAAAGUUUUUUCGGACCGGAGCGAAACCACUAUCCUCUUAAGCAAUCUGU-CAUAUGUGCGUCUACAUGGAUCCUCA	138	5'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'	ssRNA	Escherichia Coli (E. Coli)	C-terminal ribonuclease domain of Colicin E3	Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.	Addition of aptamer F2-1 in a 1000-fold excess over the colicin E3 CRD completely restored protein synthesis.	12	Nitrocellulose Filter Binding Assay	14 ± 2 nM	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	Best Candidate	N/A	N/A
ABdb_0011	15023071	2004	In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA	F3-1	GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GUACAACACAUCAUUACGGCUGCUAUUGGCUCCAAGCGUCUUUCUCCCUGGUCAAUAGUCCAGCCACCACG-CAUAUGUGCGUCUACAUGGAUCCUCA	139	5'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'	ssRNA	Escherichia Coli (E. Coli)	C-terminal ribonuclease domain of Colicin E3	Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.	N/A	12	Nitrocellulose Filter Binding Assay	4 ± 15 nM	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0012	15023071	2004	In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA	F4-1	GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGAGGGCUCGGACUCUGAUUGCCUCCCCGCACC-CAUAUGUGCGUCUACAUGGAUCCUCA	138	5'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'	ssRNA	Escherichia Coli (E. Coli)	C-terminal ribonuclease domain of Colicin E3	Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.	N/A	12	Nitrocellulose Filter Binding Assay	7 ± 1 nM	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0013	15023071	2004	In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA	F5-1	GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GUCAGCUGCUCGCGGGAUCGAUCCAUUCGGUGGCCAUGCUCCGGAAGAGGCUUCGCAAGACUCAGG-CAUAUGUGCGUCUACAUGGAUCCUCA	134	5'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'	ssRNA	Escherichia Coli (E. Coli)	C-terminal ribonuclease domain of Colicin E3	Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.	N/A	12	Nitrocellulose Filter Binding Assay	10 ± 1 nM	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0014	15023071	2004	In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA	F4-2	GGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGCUU-GACAUCUGUAAGUAAGAUUCUAUCUGCAAAGCGGUUAGGGGGGCUCGGACUCUGAUUGCCUCCCCGCACC-CAUAUGUGCGUCUACAUGGAUCCUCA	138	5'-GGTAATACGACTCACTATAGGGAGAATTCCGACCAGAAGCTT-N72-CATATGTGCGTCTACATGGATCCTCA-3'	ssRNA	Escherichia Coli (E. Coli)	C-terminal ribonuclease domain of Colicin E3	Identify aptamers that bind to colicin E3 CRD and inhibit the nuclease activity.	N/A	12	Nitrocellulose Filter Binding Assay	294 ± 170 nM	Therapeutics	SELEX	5'-Radiolabelled (32P-labeled)	N/A	N/A	N/A	N/A	N/A
ABdb_0405	24813997	2014	Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEX	G43	UAAUACGACUCACUAUAGGGAGACAAGACUAGACGCUCAA-AAUAGGAACUUAGAACAACCCUCUCCCUCAUGUAGCGAACCCGAACCAGG-UUCGACAUGAGACUCACAACAGUUCCCUUUAGUGAGGGUUAAUU	134	N/A	ssRNA	Mycobacterium Tuberculosis (H37Rv)	EsxG protein	Identify aptamers against EsxG over its homologue EsxA.	The affinity of aptamer G43 to EsxG was about ten times higher than the affinity of aptamer G78 and also showed no significant binding to EsxA.	5	Surface Plasmon Resonance (SPR)	8.04 ± 1.9 nM	Detection	SPR based SELEX	N/A	N/A	N/A	Best Candidate	N/A	N/A
ABdb_0406	24813997	2014	Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEX	G78	UAAUACGACUCACUAUAGGGAGACAAGACUAGACGCUCAA-GCGUUUAAACGUUGCACCGUCGUUGACGGGACCGCUUGAGACAUUCGCUC-UUCGACAUGAGACUCACAACAGUUCCCUUUAGUGAGGGUUAAUU	134	N/A	ssRNA	Mycobacterium Tuberculosis (H37Rv)	EsxG protein	Identify aptamers against EsxG over its homologue EsxA.	G78 showed some binding to EsxA, though a significantly lower binding signal was observed (p < 0.01) when compared to that of EsxG.	5	Surface Plasmon Resonance (SPR)	78.85 ± 9.4 nM	Detection	SPR based SELEX	N/A	N/A	N/A	N/A	N/A	N/A
ABdb_0407	24813997	2014	Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEX	G31	UAAUACGACUCACUAUAGGGAGACAAGACUAGACGCUCAA-UAACGACUCACCAACACAUCGACUAAUUUCCCUAAAUGACUUAACUGAAU-UUCGACAUGAGACUCACAACAGUUCCCUUUAGUGAGGGUUAAUU	134	N/A	ssRNA	Mycobacterium Tuberculosis (H37Rv)	EsxG protein	Identify aptamers against EsxG over its homologue EsxA.	N/A	5	Surface Plasmon Resonance (SPR)	N/A	Detection	SPR based SELEX	N/A	N/A	N/A	N/A	N/A	N/A
ABdb_0408	24813997	2014	Selection of RNA aptamers against the M. tuberculosis EsxG protein using surface plasmon resonance-based SELEX	G70	UAAUACGACUCACUAUAGGGAGACAAGACUAGACGCUCAA-ACUCGUGACACUUACGAACGACCUAUGGCGGGAGAAUCCUAGCCGCUACG-UUCGACAUGAGACUCACAACAGUUCCCUUUAGUGAGGGUUAAUU	134	N/A	ssRNA	Mycobacterium Tuberculosis (H37Rv)	EsxG protein	Identify aptamers against EsxG over its homologue EsxA.	N/A	5	Surface Plasmon Resonance (SPR)	N/A	Detection	SPR based SELEX	N/A	N/A	N/A	N/A	N/A	N/A