AptBacterialDB

Length : 101 - 110

Total records found: 23

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0138 225206542012Evaluation of the clinical value of ELISA based on MPT64 antibody aptamer for serological diagnosis of pulmonary tuberculosisMPT64-A1GGGAGCTCAGAATAAACGCTCAAA-GGGAGCTCAGAATAAACGCTCAAAAACGCTCAAGAGGCCCGGATCTTCGACATGAGGCCCGGATC-CGACATGAGGCCCGGATC1075'-GGGAGCTCAGAATAAACGCTCAAA-N35-CGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv)MPT64 antibodyIdentify aptamers against MPT64 antibody and develop a sandwich ELISA for the serological diagnosis of pulmonary TB (PTB).LOD was 2.5 mg/L, with a linear range varying from 10 mg/L to 800 mg/L.12Enzyme-Linked Immunosorbent Assay (ELISA)N/ABiosensorSELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0220 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC4CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGGCGTGGGGGCAATGCCTGCTTGTAGGCTTCCCCTGTGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.C4 showed strong specific binding to S. Typhimurium, and the increase in the fluorescence intensity of C4 was less than 30% against E. coli and S. aureus.10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0221 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC1CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGTCGGAGCCAGGATGCGAGGTCTGTAGGTCTGCGGGCGG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0223 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGGGCGTGGCTCAGAGTGGGGGTCGGTCAGTCTTGTGCGCG-GGTGGATCCATATTCCTACTCG1025'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0224 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC5CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGACGCCTGCGTGGCTGAGGCTTCGGTCTGCGCCG-GGTGGATCCATATTCCTACTCG1015'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0225 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC6CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CGTGCGGCGGGCAGGATGGGATGCTGTAGGTCTGCGGGCGCGCG-GGTGGATCCATATTCCTACTCG1045'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0226 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC7CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CGTGCGGAGCCAGGATGGGAGGTCTGTAGGTCTGCGGGGCGCG-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0228 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisC9CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGTGCGGGCCGGATGGGAGCTCTGTAGGTTGCGGGCGCGG-GGTGGATCCATATTCCTACTCG1015'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0231 239786342013Identification of Salmonella Typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysisCA3/SA-3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGGGGCCAGGATGCGAGGTCTGTAGGTCTGCGGCGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that detect and specifically bind to the surface of S. Typhimurium.N/A10Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0566 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA2CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-ACGGGCGTGGGAGGCAATGCCTTGCTTGTAGGCTTCCCCTGTGCGCG-GGTGGATCCATATTCCTACTCG1075'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0567 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA14CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACACCGCAGCAGTGGGAACGTTTCAGCCATGCAAGCATCACGCCCGT-GGTGGATCCATATTCCTACTCG1085'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence Spectroscopy3.49 ± 1.43 nMDetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0568 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersA15CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACGCGCAAACAGATTAACACTCCGCCTAAGTCTGCCGCACGC-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0570 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB3CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGCGGAGCCAGGATGGGAGGTCTGTAGGTCTGCGGGGCGTG-GGTGGATCCATATTCCTACTCG1045'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0571 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB6CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGTCGGTGTCTGCCGGGGGATGTGGAGGCTGGGTGTTGCGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.Showed specific binding to S. aureus.16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0572 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB7CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-GCGTGGGCGGGCTACCTGGCTAGTACGCCATGATGCCTGCACGCG-GGTGGATCCATATTCCTACTCG1055'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_0573 258847912015Comparison of whole-cell SELEX methods for the identification of Staphylococcus aureus-specific DNA aptamersB15CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-CACGCGCAAACAGATTAACACTCCGCCTAAGTCTGCCGCACGC-GGTGGATCCATATTCCTACTCG1035'-CGGATGCGAATTCCCTAATACGACTCACTATAGGGCGT-N40-GGTGGATCCATATTCCTACTCG-3'ssDNAStaphylococcus aureus (S. aureus) (KCCM 12103)Whole cellIdentify aptamers against bacterial surface molecules and live Staphylococcus aureus.N/A16Fluorescence SpectroscopyN/ADetectionWhole Cell-SELEXFAM LabeledN/AN/AN/AN/AN/A
ABdb_1133 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS8GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-GTTTCCGTATGCGCAGCGCCATACACCTGTCTGATCTCCCC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1143 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS18GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-TGTAGGCGTAACCTCCTACTCTGTGCACTGTCTAAAGTGAC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1144 301659342018Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. TyphimuriumS19GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-CGCGGCGTACGCTATCTTGTAACCTCCCGAACTATAACCAC-AGATAGTAAGTGCAATCT1015'-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNASalmonella Typhimurium (S. Typhimurium) ( ATCC 19585)Whole cellIdentify aptamers and develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium.N/A10Surface Plasmon Resonance (SPR)N/ADetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1333 310801982019Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens10A-S2AAAAAAAAAA-TTTTT-GCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTTGCTAA103N/AssDNAStaphylococcus aureus (S. aureus)Whole cellDeveloped a SPR-based aptasenor using polyadenine-mediated immobilization of aptamers on a gold substrate for the detection of E. coli or S. aureus.N/AN/AN/AN/ABiosensorN/A5'-PolyA (10A) and Five thymine (5T)N/AN/AN/AN/AN/A
ABdb_1773 379216342023Aptamer and DNAzyme-Functionalized Cu-MOF Hybrid Nanozymes for the Monitoring and Management of Bacteria-Infected WoundsAptamerGGGAAAGGGAAAGGGAAAGGG-TTTTTT-ATCCAGACGTGACGCAGCATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTGTGGACACGGTGGCTTAGTA102N/AssDNAMethicillin-resistant Staphylococcus aureus (MRSA)MRSA surface componentsG-quadruplex/hemin DNAzyme-aptamer probes and tannic acid-chelated Au nanoparticle (Au-TA)-decorated Cu-based MOF nanosheets (termed GATC) with triple-enzyme activities were developed for visual detection and efficient antibacterial therapy.~99.7% MRSA inactivation at 3 μg/mL, with simultaneous colorimetric monitoring of infection.N/AN/AN/ATherapeuticsN/A5'-ThiolatedAt 50 μg/mL GATC, the cells showed high viability (>90%), indicating negligible cytotoxicity and good cytocompatibility. The hemolysis rate of the GATC nanozyme was low (<5%).N/AN/AN/AN/A
ABdb_1811 https://doi.org/10.3390/fishes81004772023A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in AquacultureAptamerGAGAGAGAATATAAGGGAAAAAAAATCAGTCGCTTCGCCGTCTCCTTCGGGGGCGCGGTGAGGGGCTGCACAAGAGGGAGGCACAAGAGGGAGACCCCAGAGGG104N/AssDNAVibrio Alginolyticus (ATCC 17749)Whole cellDeveloped a method using an HCR-based multivalent aptamer (multi-Apt) for the detection of Vibrio alginolyticus.Obtained a linear range from 10 to 10(7) CFU/mL, and the limit of detection (LOD) is 3 CFU/mL.N/AN/AN/ADetectionN/ASA-HRP or 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1852 377516322024A novel paper-based electrochemiluminescence biosensor for non-destructive detection of pathogenic bacteria in real samplesAptamerGCAATGGTACGGTACTTCCTCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGTCAAAAGTGCACGCTACTTGCTAACCCCCCTTAATCCCCCC104N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 29213)Whole cellDeveloped a paper-based electrochemiluminescence (ECL) aptasensor based on a Ru-MOF-5 NFs modified Indium tin oxide (ITO) electrode for the detection of S. aureus.Exhibited a linear range from 5 to 10(8) CFU/mL, and a limit of detection (LOD) of 1 CFU/mL.N/AN/AN/ABiosensorN/A5'-COOH (Carboxylated)N/AAfter 9 days at 4°C, the ECL signal of aptsensor retained 95.8% of its initial value, suggesting satisfactory storage stability.N/AN/AN/A