AptBacterialDB

Determination of affinity method : Saturation Binding Assay

Total records found: 19

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0356 251855032014Selection of peptidoglycan-specific aptamers for bacterial cells identificationAntibac1TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC555'-TCGCGCGAGTCGTCTG-N24-CCGCATCGTCCTCCC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923) and Escherichia Coli (E. Coli) TOP10PeptidoglycanIdentify aptamers that bind to the peptidoglycan of bacterial cells.High binding affinity for S. aureus, as the radioactivity of Antibac1 was 28 times higher than the negative control.5Saturation Binding Assay0.415 + 0.047 μMDetectionSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0357 251855032014Selection of peptidoglycan-specific aptamers for bacterial cells identificationAntibac2TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC555'-TCGCGCGAGTCGTCTG-N24-CCGCATCGTCCTCCC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923) and Escherichia Coli (E. Coli) TOP10PeptidoglycanIdentify aptamers that bind to the peptidoglycan of bacterial cells.High binding affinity for S. aureus, as the radioactivity of Antibac2 was 22 times higher than the negative control.5Saturation Binding Assay1.261 + 0.280 μMDetectionSELEX5'-Radiolabelled (32P-labeled)N/AN/AN/AN/AN/A
ABdb_0935 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.1AGCGC(U^y)CGCGCGGCG(U^y)GC-ACAACAAA(U^y)G(U^y)GACCA(U^y)GCGA(U^y)(U^y)CCCCA(U^y)A(U^y)CCAGGCACA-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0936 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.2AGCGC(U^y)CGCGCGGCG(U^y)GC-GA(U^y)GCG(U^y)G(U^y)GG(U^y)G(U^y)GGAG(U^y)(U^y)G(U^y)G(U^y)GAG(U^y)GG(U^y)C(U^y)GCG(U^y)(U^y)G(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0937 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.5BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)(U^y)CA(U^y)GCCC(U^y)G(U^y)G(U^y)C(U^y)(U^y)GC(U^y)C(U^y)(U^y)G(U^y)GAG(U^y)(U^y)G(U^y)(U^y)G(U^y)G(U^y)CA-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0938 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.8AGCGC(U^y)CGCGCGGCG(U^y)GC-G(U^y)G(U^y)G(U^y)A(U^y)GCG(U^y)(U^y)CA(U^y)G(U^y)GG(U^y)GAGG(U^y)C(U^y)(U^y)GCG(U^y)C(U^y)G(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0939 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.10AGCGC(U^y)CGCGCGGCG(U^y)GC-GAG(U^y)G(U^y)G(U^y)G(U^y)GGG(U^y)CCGAG(U^y)GGG(U^y)GG(U^y)CAGGG(U^y)(U^y)(U^y)G(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.Showed the highest % binding to the DH5α cells, with per cent bound ranging from 2.9 to 21%.12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0940 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.14BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)C(U^y)G(U^y)G(U^y)GCGG(U^y)G(U^y)(U^y)A(U^y)GCGGG(U^y)(U^y)GGG(U^y)(U^y)G(U^y)(U^y)(U^y)G(U^y)G(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.Showed the highest % binding to the DH5α cells, with per cent bound ranging from 2.9 to 21%.12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0941 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.16BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)(U^y)(U^y)GCG(U^y)CCGGG(U^y)(U^y)(U^y)A(U^y)GCGGG(U^y)(U^y)G(U^y)C(U^y)CG(U^y)G(U^y)C(U^y)G(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0942 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.17BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)C(U^y)(U^y)G(U^y)(U^y)GGG(U^y)CGACGG(U^y)C(U^y)G(U^y)(U^y)CA(U^y)G(U^y)GGG(U^y)G(U^y)(U^y)G(U^y)CCC-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0943 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.18BGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)(U^y)G(U^y)G(U^y)(U^y)CACG(U^y)CA(U^y)(U^y)(U^y)CGCAC(U^y)CC(U^y)C(U^y)CAGC(U^y)ACG(U^y)(U^y)(U^y)(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.Showed the highest % binding to the DH5α cells, with per cent bound ranging from 2.9 to 21%.12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0944 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.24AGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)G(U^y)G(U^y)(U^y)(U^y)G(U^y)GG(U^y)ACGAGCG(U^y)G(U^y)G(U^y)GGA(U^y)GG(U^y)CCG(U^y)G(U^y)CA-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC765'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_0945 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.28AGCGC(U^y)CGCGCGGCG(U^y)GC-(U^y)CC(U^y)CGCG(U^y)(U^y)(U^y)GGA(U^y)(U^y)CA(U^y)G(U^y)(U^y)GG(U^y)(U^y)(U^y)G(U^y)CGG(U^y)G(U^y)A(U^y)(U^y)G(U^y)-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC775'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.Showed the highest % binding to the DH5α cells, with per cent bound ranging from 2.9 to 21%.12Saturation Binding Assay27.4 ± 18.7 nMDetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP) and Radiolabelled ([γ-32P]-labeled)N/AN/ABest CandidateN/AN/A
ABdb_0946 280099142017Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria8.30AGCGC(U^y)CGCGCGGCG(U^y)GC-ACAGAAAG(U^y)G(U^y)GGCCA(U^y)G(U^y)G(U^y)(U^y)G(U^y)G(U^y)CCC(U^y)GGCAGG(U^y)(U^y)AG(U^y)(U^y)(U^y)A-C(U^y)G(U^y)(U^y)GGCGCAGGCCGACGC815'-GCGCTCGCGCGGCGTGC-40N-CTGTTGGCGCAGGCCGACGC-3'ssDNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify modified aptamers that were isolated against Escherichia coli DH5α cells.N/A12Saturation Binding AssayN/ADetectionWhole Cell-SELEXPhenol-modified dUTP (dU^yTP)N/AN/AN/AN/AN/A
ABdb_1220 307797542019C4-HSL aptamers for blocking quorum sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysisA16CCATCCACACTCCGCAAGTGGGGAGGGGAGAGACGACGATCCTGTGGGTTTTCTGCAGTGAGTCGTGTTTTCGACTTATTGCGTCGGCTGCCTCTACAT995'-CCATCCACACTCCGCAAG-N30-TTTT-hybridsequence-TTTT-N10-CGTCGGCTGCCTCTACAT-3'ssDNAPseudomonas AeruginosaC4-HSL of the rhl system quorum sensing moleculeIdentify aptamers that inhibit biofilm formation and quorum sensing.Biofilm formation by P. aeruginosa was reduced by about 1/3.10Saturation Binding Assay28.47 nMTherapeuticsStructure Switching SELEXN/AAptamers caused no effect on bacterial growth.N/AN/AN/AN/A
ABdb_1221 307797542019C4-HSL aptamers for blocking quorum sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysisA46CCATCCACACTCCGCAAGTATGAGGACAGTTGGAGGGCGCGCCGTGTTTTTTCTGCAGTGAGTCGTGTTTCCTCGCTCACGCGTCGGCTGCCTCTACAT995'-CCATCCACACTCCGCAAG-N30-TTTT-hybridsequence-TTTT-N10-CGTCGGCTGCCTCTACAT-3'ssDNAPseudomonas AeruginosaC4-HSL of the rhl system quorum sensing moleculeIdentify aptamers that inhibit biofilm formation and quorum sensing.Biofilm formation by P. aeruginosa was reduced by about 1/3.10Saturation Binding AssayN/ATherapeuticsStructure Switching SELEXN/AAptamers caused no effect on bacterial growth.N/AN/AN/AN/A
ABdb_1222 307797542019C4-HSL aptamers for blocking quorum sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysisA1CCATCCACACTCCGCAAGTTTTAGGAGTAGTGGTGTGGGGACCGACTCTTTTCTGCAGTGAGTCGTGTTTTCGAGTCATCCCGTCGGCTGCCTCTACAT995'-CCATCCACACTCCGCAAG-N30-TTTT-hybridsequence-TTTT-N10-CGTCGGCTGCCTCTACAT-3'ssDNAPseudomonas AeruginosaC4-HSL of the rhl system quorum sensing moleculeIdentify aptamers that inhibit biofilm formation and quorum sensing.Biofilm formation by P. aeruginosa was reduced by about 1/3.10Saturation Binding AssayN/ATherapeuticsStructure Switching SELEXN/AAptamers caused no effect on bacterial growth.N/AN/AN/AN/A
ABdb_1223 307797542019C4-HSL aptamers for blocking quorum sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysisA2CCATCCACACTCCGCAAGTGAGATGTGGTGTAGGCAACTCGGCATAGATTTTCTGCAGTGAGTCGTGTTTTTTGACACGGCCGTCGGCTGCCTCTACAT995'-CCATCCACACTCCGCAAG-N30-TTTT-hybridsequence-TTTT-N10-CGTCGGCTGCCTCTACAT-3'ssDNAPseudomonas AeruginosaC4-HSL of the rhl system quorum sensing moleculeIdentify aptamers that inhibit biofilm formation and quorum sensing.Biofilm formation by P. aeruginosa was reduced by about 1/3.10Saturation Binding AssayN/ATherapeuticsStructure Switching SELEXN/AAptamers caused no effect on bacterial growth.N/AN/AN/AN/A
ABdb_2119 302453242018Aptamer functionalized MoS2-rGO nanocomposite based biosensor for the detection of Vi antigenAnti-Vi aptamers populationN/AN/A5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNASalmonella TyphiVi polysaccharide antigen (Vi antigen)Developed a novel aptamer functionalized MoS2-rGO-based electrochemical aptasensor for Vi polysaccharide antigen-mediated detection of enteric fever.Responded linearly in the range between 0.1 ng/mL to 1000 ng/mL with a detection limit of 100 pg/mL.6Saturation Binding Assay638.6 nMBiosensorSELEX5'-ThiolatedN/AN/AN/AN/AN/A