Determination of affinity method : Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0090 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerTwo Arm ( from clone I-1)GGGUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAUAGAUAGUAA645'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Specifically bound to the lipopolysaccharide, which includes the O antigen from the O157:H7 strain, but not to the LPS from the K12 strain.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)~110 nMDetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/ABest CandidateN/AN/A
ABdb_0091 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerClone I-1GGGAUACCAGCUUAUUCAAUU-UGAUUCCAUCUUCCUGGACUGUCGAAAAUUCAGUAUCGGGAGGUUACGUAUUUGGUUUAU-AGAUAGUAAGUGCAAUCU995'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Bound to the E. coli O157:H7 strain 2.8-fold better than the control.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/AN/AN/AN/A
ABdb_0092 221662022011In vitro selection of Escherichia coli O157:H7-specific RNA aptamerClone III-2GGGAUACCAGCUUAUUCAAUU-CGCACUGUUUUGCCCAGCCGUGUGAAAUUGAUUAGACGUUUUGUAUGCGAUUUCGCCUUG-AGAUAGUAAGUGCAAUCU995'-GGGAUACCAGCUUAUUCAAUU-N60-AGAUAGUAAGUGCAAUCU-3'ssRNAEscherichia Coli (E. Coli) O157:H7(ATCC 43895)Whole cellIdentify aptamers that bind to and distinguish between the virulent serotype and the nonpathogenic strain of E.coli.Bound to the E. coli O157:H7 strain 2.3-fold better than the control.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSubtractive SELEX2'-Fluoro pyrimidines (2'-F-RNA) and A(16) extended on the 3'N/AN/AN/AN/AN/A
ABdb_0236 236895052013Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticlesSA17TCCCTACGGCGCTAAC-CCCCCCAGTCCGTCCTCCCAGCCTCACACC-GCCACCGTGCTACAAC625'-TCCCTACGGCGCTAAC-N30-GCCACCGTGCTACAAC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC: 6538DR)Whole cellIdentify aptamers that binds to and recognize strains of S.aureus using aptamer-conjugated gold nanoparticles.LOD was 312 bacterial cells, and the lower limit of detection of the instrument was 63 ± 21 GNPs/μl for 100-nm particles and 508 ± 176 GNPs/μl for 60-nm particles.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)35 nM and 3.03 nM for SA17-GNPsBiosensorWhole Cell-SELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0237 236895052013Rapid single cell detection of Staphylococcus aureus by aptamer-conjugated gold nanoparticlesSA61TCCCTACGGCGCTAAC-CTCCCAACCGCTCCACCCTGCCTCCGCCTC-GCCACCGTGCTACAAC625'-TCCCTACGGCGCTAAC-N30-GCCACCGTGCTACAAC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC: 6538DR)Whole cellIdentify aptamers that binds to and recognize strains of S.aureus using aptamer-conjugated gold nanoparticles.LOD was 1250 bacterial cells, and the lower limit of detection of the instrument was 63 ± 21 GNPs/μl for 100-nm particles and 508 ± 176 GNPs/μl for 60-nm particles.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)129 nM and 9.9 nM for SA61-GNPsBiosensorWhole Cell-SELEXBiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0273 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2GGGAGAGCGGAAGCGUGCUGGG-UAGUGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGA-CAUAACCCAGAGGUCGAUGGUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20.27 ± 4.372 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0274 236769112013In vitro selection of RNA aptamer specific to Salmonella typhimuriumClone I-2 Truncated 43-merGUGAGAGCCGUGAGUGAAAGGCCGCGACAAAGAUCGGAC395'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNASalmonella Typhimurium (S. Typhimurium) (ATCC 15277)OmpC proteinIdentify an RNase-resistant RNA aptamer that binds to the OmpC protein of S. Typhimurium.Bound only to Sal.Typhimurium, but not to S. aureus, E. coli O157:H7 and to that does not express the OmpC protein.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)27.64 ± 4.784 nMDetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA) and 5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0275 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #2GGGAGAGCGGAAGCGUGCUGGGCC-GGGAGUUUUGAUACGGCUUCAUGCAGUAAUGUUUUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.The level of binding of #2 RNA aptamer to S. aureus was 1.6-fold higher than that of the control aptamer.6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/ABest CandidateN/AN/A
ABdb_0276 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #10GGGAGAGCGGAAGCGUGCUGGGCC-GUUAAUACGGUGUCUUUUUCGGUCGUGUAUAAAACGGAAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0277 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #14GGGAGAGCGGAAGCGUGCUGGGCC-UAGGACAGUUCGUCCUCAUUACAUCGCCGCCUAACACAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0278 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #16GGGAGAGCGGAAGCGUGCUGGGCC-UUAGAAGUAGCCUGCUACGCAUGGUCGACUCAAGAAUCG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0279 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #18GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0280 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #21GGGAGAGCGGAAGCGUGCUGGGCC-AGUCUGACACGUAGACAGUUCUAUUACUUACGUCGAGA-CAUAACCCAGAGGUCGAUGGAUCCCC885'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0281 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #23GGGAGAGCGGAAGCGUGCUGGGCC-ACAGUGUUCUAAUGCGACAAUGGAGUCUGUGGCAAAGUGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0282 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #24GGGAGAGCGGAAGCGUGCUGGGCC-UCUCAGGCCGACAUUCUGAGAACGCGAGGCGUAUUGAAG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0283 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #26GGGAGAGCGGAAGCGUGCUGGGCC-UUCAAGUAGGGGCGGUUUACUAUCUGGAUCUUGUAGUUAU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0284 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #07GGGAGAGCGGAAGCGUGCUGGGCC-ACUUGGGGACGACGAGUAGAUAGUAAGGUGGAGACCUGGU-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0285 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #06GGGAGAGCGGAAGCGUGCUGGGCC-UAUGACAUAAGGUGGGCUGGGAAGCUAGAGCAUGUAAGG-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0286 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #05GGGAGAGCGGAAGCGUGCUGGGCC-GUGAAGAAAAAGGGGCGGAUUGGGUAGUAGGGAGGAGAUC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0287 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone #04GGGAGAGCGGAAGCGUGCUGGGCC-AGGAUAGGGGAUGAAGAAAAAAAGAAGGGUGCCGUGGCGC-CAUAACCCAGAGGUCGAUGGAUCCCC905'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0288 https://doi.org/10.1007/s13213-013-0720-z2013In vitro selection of RNA aptamer specific to Staphylococcus aureusClone G1GGGAGAGCGGAAGCGUGCUGGGCC-UCCGAACAGCGGAAGGUGGUUCGAAGUUGGGGCUUUGGA-CAUAACCCAGAGGUCGAUGGAUCCCC895'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3'ssRNAStaphylococcus aureus (S. aureus)Teichoic acidIdentify aptamers that bind to cell surface-exposed epitopes of the teichoic acid.N/A6Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_0358 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-27ACGGCTCGCACTCTCTGATTT-GGCATAGCTGCCGGGAGGGGGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.The binding signal of EcA5-27 for E. coli NSM59 was notably higher (~ 1.5-fold) than for laboratory strains of E. coli, and 8.5- and 56-fold to additional uropathogenic isolates compared with laboratory strains.5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)110 nMDetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/ABest CandidateN/AN/A
ABdb_0359 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-01ACGGCTCGCACTCTCTGATTT-CGGCACCCCGTCGCTATGTTGACC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0360 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-02ACGGCTCGCACTCTCTGATTT-GGGGAGGTGGCGACCGCTTCTCAG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0361 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-03ACGGCTCGCACTCTCTGATTT-GGGGTCAGATATTAAACCGTGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0362 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-04ACGGCTCGCACTCTCTGATTT-GGGAGAGGGAGTGGTCTGGGAGAG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0363 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-05ACGGCTCGCACTCTCTGATTT-GCGGGCTTCGACACAGTGGGGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0364 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-06ACGGCTCGCACTCTCTGATTT-GGGAGGGGCGGCGAAGGAGTGGCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0365 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-07ACGGCTCGCACTCTCTGATTT-GGAAGCGGTGGGGATCGTGTGTGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0366 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-08ACGGCTCGCACTCTCTGATTT-GGGAGCAAATCCGGAATGTGGGGC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0367 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-09ACGGCTCGCACTCTCTGATTT-GGCGGAGGGGTTCGGGGTTGGCGC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0368 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-10ACGGCTCGCACTCTCTGATTT-GGCGGGGCGTGGGGGATGTGTGTG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0369 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-11ACGGCTCGCACTCTCTGATTT-GGAATGCGAAGTGTGGCCTAGGGC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0370 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-12ACGGCTCGCACTCTCTGATTT-GGGCGGGGGGGGATTCCGAGGCGC-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0371 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-13ACGGCTCGCACTCTCTGATTT-GGGGGGGTGGCATTTTGGGGTGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0372 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-14ACGGCTCGCACTCTCTGATTT-GCGGGGAAAGAGAAGGAAGCGTCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0373 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-15ACGGCTCGCACTCTCTGATTT-GGGCCCGAGTGGCGGTAGTTTCAG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0374 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-16ACGGCTCGCACTCTCTGATTT-GGGGGGTGGTGAAGGCCTGGGGGA-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0375 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-17ACGGCTCGCACTCTCTGATTT-GGGCCGGAGGGGCGCCTGCACCCA-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0376 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-18ACGGCTCGCACTCTCTGATTT-GGGGTTAGGCGAGGGGGGTGGGTG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0377 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-19ACGGCTCGCACTCTCTGATTT-CGGACGGTAGGGAAGGGGGGGGCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0378 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-20ACGGCTCGCACTCTCTGATTT-GGCGAGGCAGGGTGCGGGGGCCCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0379 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-21ACGGCTCGCACTCTCTGATTT-GCGTGTGGTGGGTGAGGGGTCTGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0380 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-22ACGGCTCGCACTCTCTGATTT-GGGGATAGCAGGACAATGAGGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0381 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-23ACGGCTCGCACTCTCTGATTT-GGCCGCGTGTGTGTCCGACTGGTG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0382 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-24ACGGCTCGCACTCTCTGATTT-GGGCGAGGAGAGAGGCGGAGGGCG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0383 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-25ACGGCTCGCACTCTCTGATTT-GCCGTGGTGTGTGTGATGGTCGGT-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0384 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-26ACGGCTCGCACTCTCTGATTT-GGCTTGGCTCCTCACGGGGGGTGA-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0385 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-28ACGGCTCGCACTCTCTGATTT-GGAGGGGTTGACCATGACCGGGGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0386 250084642014Selection of DNA aptamers against uropathogenic Escherichia coli NSM59 by quantitative PCR controlled Cell-SELEXEcA5-29ACGGCTCGCACTCTCTGATTT-GGGGGAAGGGCCAATGGATTGTGG-TTTACTCCTGCGTGCTTCTCA665'-ACGGCTCGCACTCTCTGATTT-N24-TTTACTCCTGCGTGCTTCTCA-3'ssDNAEscherichia Coli (E. Coli) NSM59 Uropathogenic strainWhole cellIdentify aptamers that bind specifically to E. coli NSM59 cells.N/A5Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FITC and 5'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0506 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 36CCGTCAGCCGAGGACCACAC-TTGGTTGCTGAATCCCCTCGTCTTGGCTTTCTTTGTCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.Mtb36 aptamer bound to M. tuberculosis H37Ra bacteria more than 4 times for M. bovis and 70 times for E. coli bacteria.7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)5.09 ±1.43 nMDetectionWhole Cell-SELEXN/AN/AN/ABest CandidateN/AN/A
ABdb_0507 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 17CCGTCAGCCGAGGACCACAC-GCGTCAATATGCTCGAGTCCCTTACTCCGTAATCTTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0508 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 57CCGTCAGCCGAGGACCACAC-GAGGCGGGGCGTACATGATGGAGGTGTTGTGTTCTTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0509 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 25CCGTCAGCCGAGGACCACAC-ATAGGAAGTATGCGGTCGTAGTTAATCGCCTGTCCTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0510 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 32CCGTCAGCCGAGGACCACAC-CTGGGCTCACTCACTGGCGTATCATTCGTCCGCGGTGGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0511 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 3CCGTCAGCCGAGGACCACAC-CGCTTCACGTGTCAGTGAATTTTCTCCATCGTTTGGTGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0512 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 40CCGTCAGCCGAGGACCACAC-TGTAGCGCATCTACGGGCTCTTCATTACGTCTATATCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0513 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 33CCGTCAGCCGAGGACCACAC-TTCACACACGGGTATAAACGCTCTGGTATGTCAAGCCGGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0514 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 35CCGTCAGCCGAGGACCACAC-TCGGACCTAGTGCTAGGGTCTCTAAGAAGTATCGGGGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0515 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 49CCGTCAGCCGAGGACCACAC-TTCTCCGATCTTAGTCAACTTGGACCATGAATGCGGGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0516 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 46CCGTCAGCCGAGGACCACAC-TATTTACGCTGACCGACGATTTTCTATCAGAGTGCCGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0517 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 13CCGTCAGCCGAGGACCACAC-CGATCACTGTACAGTCCTGGATAAGCCGTTCTTTCCGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0518 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 11CCGTCAGCCGAGGACCACAC-CGCCGGCCTTCTTACAAGACCTGTTCAATTCCCAGTGTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0519 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 50CCGTCAGCCGAGGACCACAC-AGGCTCACGCCCTGTCAATACTGCCTCTTGTCCTTCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0520 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 38CCGTCAGCCGAGGACCACAC-ATTCGGCTGAAGACCCGAGCCGGTCATCCGGTGTTCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0521 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 28CCGTCAGCCGAGGACCACAC-CTCCATCACGTGTAGTCAGCTGACCATTGATCGTGCCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0522 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 8CCGTCAGCCGAGGACCACAC-CTATCCCGTGGTCCATTGCTTTTCCCGGTCTCTTCTCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0523 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 52CCGTCAGCCGAGGACCACAC-CTTTGTGCCGGGAAAAGACTGGCCTGTGTTGACTTGCTGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0524 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 2CCGTCAGCCGAGGACCACAC-CGGCGCTTTTCTCATCCTACCTCCGCTCTTACCTGTATGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0525 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 9CCGTCAGCCGAGGACCACAC-GCCGTGCTGAGTCTGTCAGCAGTTTGCTAGTCTTCCCTGC-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0526 265411622015Selection of Nucleic Acid Aptamers Specific for Mycobacterium tuberculosisMtb 44CCGTCAGCCGAGGACCACAC-TTACACGAAGGCTCGGCCCATCTCTCTCGTGTCACTTCGG-TTGGGTGCAATGAAT755'-CCGTCAGCCGAGGACCACAC-N40-TTGGGTGCAATGAAT-3'ssDNAMycobacterium Tuberculosis (H37Ra)Whole cellIdentify aptamer that specifically binds to M. tuberculosis H37Ra.N/A7Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_0761 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-52CATACGATTTAGGTGACACTATAG-CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.P12-52 bound strongly to all three Gram-positive bacteria tested (S. aureus, E. faecalis and E. freudini).12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)11.97 ± 2.94 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0762 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-31CATACGATTTAGGTGACACTATAG-CCCTCCGGGGGGGGGGGTCATCGGGATACCTGGTAAGGATA-ATTTCTCCTACTGGGATAGGTGGA895'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.The binding of P12-31 was 10-fold stronger than that of the random library.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)87.03 ± 17.32 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0763 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-55CATACGATTTAGGTGACACTATAG-CCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.The binding of P12-55 to E. coli cells was more than 100-fold stronger than that of the random library.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)161.0 ± 34.74 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0764 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-17CATACGATTTAGGTGACACTATAG-GACGGTGGCAGGGAAAGGGGTCGGGCATATGGCGGAGGGG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.P12-17 could only bind to E. faecalis. The binding of P12-17 was 10-fold stronger than that of the random library.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)56.33 ± 15.87 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0765 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-11CATACGATTTAGGTGACACTATAG-CCCTCCGGGGGGGTCATCGGGATACCTGGTAAGGATA-ATTTCTCCTACTGGGATAGGTGGA855'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0766 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-21CATACGATTTAGGTGACACTATAG-CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0767 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-9CATACGATTTAGGTGACACTATAG-TGCGGGCGGAGGACACGGACCCGTATGGGAGCAATGCACG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0768 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-15CATACGATTTAGGTGACACTATAG-GGCCACCCACAGGCACTCCGCTCATGAATCGTGGAGTCGG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0769 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-30CATACGATTTAGGTGACACTATAG-CACCACGGACACGATCCCAAGCTAGGAGGTGCGGCGGGGT-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0770 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-48CATACGATTTAGGTGACACTATAG-TGGCACAGCACGTCGCACGGTCCCCGGGAGGTGTTCACTG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0771 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-51CATACGATTTAGGTGACACTATAG-TCGCGAGTGCGTGTACGCCACACATCACAAAAGGGGTGTG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0772 271048342016Isolation of an Aptamer that Binds Specifically to E. coliP12-66CATACGATTTAGGTGACACTATAG-GGCAACATTCAGACATACCAGTACCCACTCGGACTTCCCG-ATTTCTCCTACTGGGATAGGTGGA885'-CATACGATTTAGGTGACACTATAG-N40-ATTTCTCCTACTGGGATAGGTGGA-3'ssDNAEscherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers that bind to E.coli and to three MNEC clinical isolates from septic patients.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0776 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc3 (31) -TruncatedUUCAAUUGCACGAAUUUGCUGUGUUUUUGGG315'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.Ec3(31)-biotin-streptavidin magnetic separation has LOD of 1.3 × 10(6) CFU/ml, Optical analytic technique has LOD of 5 × 10(4) CFU/ml, and Electrochemical Impedance Spectroscopy (EIS) has LOD of 2 × 10(4) CFU/mL of E. coli cells.12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)225 nMBiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-Biotinylated or Thiolated or Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_0777 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc3AUACCAGCUUAUUCAAUUGCACGAAUUUGCUGUGUUUUUGGGGGGGUCGGGGAGUAUAAGAUAGUAAGUGCAAUCU765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0778 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc2AUACCAGCUUAUUCAAUUCUUCUUUGAUCUGCUCGUAUCAUGGGACGGGAGGGUAUAGAUAGUAAGUGCAAUCU745'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0779 278711712016Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection FormatsEc5AUACCAGCUUAUUCAAUUCGAAUUUAAGUUUCUUUUCUGGUAUAGUGGUUGGGUGGGUAAGAUAGUAAGUGCAAUCU775'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssRNAEscherichia Coli (E. Coli) DH5αWhole cellIdentify an aptamer that binds specifically to E. coli, and use the minimized Ec-3(31) aptamer to develop detection assays.N/A12Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA) or 3'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0918 281195142017High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis AgentsAntibac1TCGCGCGAGTCGTCTG-GGGACAGGGAGTGCGCTGCTCCCC-CCGCATCGTCCTCCC55N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922), Klebsiella Pneumoniae (ATCC 27853), Proteus Mirabilis (ATCC 00557), Pseudomonas Aeruginosa (ATCC 700603), Staphylococcus aureus (S. aureus) (ATCC 29213), Streptococcus Pneumoniae (ATCC 23855), and Enterococcus Faecium (ATCC 29212), and Proteus Vulgaris, Morganella Morganii, Citrobacter Freundii, and Acinetobacter BaumanniiPeptidoglycanDevelop generic probes as biological recognition elements in biosensors for sepsis diagnosis in the clinical setting.Antibac1 bound with higher efficiency to both gram-positive (S. aureus, S. penumoniae, L. monocytogenes) and gram-negative (A. baumannii, C. freudii, K. pneumoniae, P. aeruginosa, P. mirabilis).N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)268.50 ± 54.34 nM (for A. baumannii); 51.74 ± 11.75 nM (for L. monocytogenes); 31.82 ± 4.38 nM (for E.coli); 170.10 ± 32.13 nM (for S.aureus) and 256.10 ± 47.89 nM (for K. pneumoniae)DiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_0919 281195142017High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis AgentsAntibac2TCGCGCGAGTCGTCTG-GGGGACTAGAGGACTTGTGCGGCC-CCGCATCGTCCTCCC55N/AssDNAEscherichia Coli (E. Coli) (ATCC 25922), Klebsiella Pneumoniae (ATCC 27853), Proteus Mirabilis (ATCC 00557), Pseudomonas Aeruginosa (ATCC 700603), Staphylococcus aureus (S. aureus) (ATCC 29213), Streptococcus Pneumoniae (ATCC 23855), and Enterococcus Faecium (ATCC 29212), and Proteus Vulgaris, Morganella Morganii, Citrobacter Freundii, and Acinetobacter BaumanniiPeptidoglycanDevelop generic probes as biological recognition elements in biosensors for sepsis diagnosis in the clinical setting.Antibac2 bound with higher efficiency to the gram-positive species S. aureus and E. faecium and to the gram-negative species (A. baumannii, C. freudii, K. pneumoniae, M. moraganii, P. aeruginosa, P. mirabilis, and E. coli).N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)71.92 ± 9.74 nM (for A. baumannii); 54.19 ± 12.09 nM (for L. monocytogenes); 62.43 ± 11.97 nM (for E.coli); 194.90 ± 38.55 nM (for S.aureus) and 195.90 ± 42.91 nM (for K. pneumoniae)DiagnosticN/AN/AN/AN/AN/AN/AN/A
ABdb_1386 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF501TAGGGAAGAGAAGGACATATGAT-TTTCTCAACGGGACCATCACTTACCTCAAGTACTTGGACG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1387 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF502TAGGGAAGAGAAGGACATATGAT-CCGGCTATCTCCCTACCGTGGCCGAGTACCTCAAACGTTT-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1388 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF503TAGGGAAGAGAAGGACATATGAT-GTCAACTCATTTATGGTGCTCCTCGTACCTCAGGTGGTTA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1389 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF504TAGGGAAGAGAAGGACATATGAT-GGCCATACCTCGTGCCTTCTGTGATCATCTCTATCAATTG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1390 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF505TAGGGAAGAGAAGGACATATGAT-CCTCTCTCTTACTGCTACTGGGCAGGGTACTCAATTACGT-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1391 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF506TAGGGAAGAGAAGGACATATGAT-CGGTCCCGACTCAATATTGTTCCCTCCCCTTATCAGGCGG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1392 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF507TAGGGAAGAGAAGGACATATGAT-TCCTCTAATCAACTCTATGCCTTATCCCCTTGGTCAGGAC-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1393 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF508TAGGGAAGAGAAGGACATATGAT-ACTGGCCTTGACACCCTGTTGTGGCTTGATGACAATAACA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.EF508 exhibited more than 20- to 800-fold higher binding to E. faecalis target cells than to non-target cells.11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)37 ± 4 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1394 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF509TAGGGAAGAGAAGGACATATGAT-CCTCACTCTTGACCCAAAGTGCATGCTCTATTCATTCGGA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1395 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF510TAGGGAAGAGAAGGACATATGAT-GCTTCTGTGCACATTAAGGCACTCGTCTTCACTGTGGTTC-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1396 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF511TAGGGAAGAGAAGGACATATGAT-CCTAACTCACTTACCAGCACGAGGTGCCTGTACCATCAAT-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1397 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF512TAGGGAAGAGAAGGACATATGAT-CTCTCATCACAGGAATTTGAATTTCCCTTGTGGACAGTAA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1398 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF513TAGGGAAGAGAAGGACATATGAT-GATGTGAATTCCGTCCCTTGGTCAGACACTTCAACACCGG-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1399 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF514TAGGGAAGAGAAGGACATATGAT-TCTCGACGCTATGATCAAGACGCAGTATGATGGCACATCA-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1400 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF515TAGGGAAGAGAAGGACATATGAT-TTAACCCTCATTTAATGGCCGCGTCAATCCGCAAAGGGTC-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1401 332623792020DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencingEF516TAGGGAAGAGAAGGACATATGAT-TTCCTTCGCAGGACACCGATGGCCAGGCGCGAGTCAATAT-TTGACTAGTACATGACCACTTGA865'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAEnterococcus faecalis (E. faecalis) DSM-20478Whole cellIdentify aptamers against E.faecalis and can discriminate them from other species.N/A11Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1444 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.01 (ID 5)TTTTTAAGCCCACAGACGWYCGGCAGGCACAGTYYGTCAAGGXCGYGCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitW = indole‐dU,Y = phenol‐dU,X = amine‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1445 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.02 (ID 6)TTTTTAAGCCCACCYCGCYGTGCAAGGCGAACGCCATCAGTGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitY = phenol‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1446 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.03 (ID 7)TTTTTAAGCCCACCYCCGWYCGAAGGCCACAGCYCATGCGCGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitY = phenol‐dU,W = indole‐dU, five additional Ts at the 5'-end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1447 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.04 (ID 8)TTTTTAACACGACCCCACYGTGCGAGCCGAACACCACCACGGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitY = phenol‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1448 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.05 (ID 9)TTTTTAACACGACXYAGCYGTGWGGGCCGAACACCAGGCACGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitX = amine‐dU,Y = phenol‐dU, five additional Ts at the 5'‐end, and CCATG at the 3′‐end, five additional Ts at the 5′‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1449 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.06 (ID 12)TTTTTAACACGACAGCAGWYCGGCGGGCACAGTGCGTGCGAGXCGYGCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.Aptamer ID 12 showed specific binding to Vp.2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitW = indole‐dU,Y = phenol‐dU,X = amine‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/ABest CandidateN/AN/A
ABdb_1450 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.07 (ID 13)TTTTTAACACGACCAWACYGTGGCAGACGAACGCCGTCACAGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitY = phenol‐dU,W = indole‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1451 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.08 (ID 14)TTTTTAAGCCCACGCGGCYGTGAGXCGCACAGCCAWAGCACGTGGGCCCATG52N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitW = indole‐dU,Y = phenol‐dU,X = amine‐dU, five additional Ts at the 5'‐end, and 3'-CCATGN/AN/AN/AN/AN/A
ABdb_1452 327246452020Selection of aptamers targeted to food-borne pathogenic bacteria Vibrio parahaemolyticusS.184004.T1.09 (ID 15)GCCCACTGAACTGTGGCGGGCACAGGATGTGGAAGTGGGC40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify high‐affinity aptamers that specifically recognize Vp.N/A2Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionAM X‐aptamer kitN/AN/AN/AN/AN/AN/A
ABdb_1608 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su12GGGAGUCGACCGACCAGAA-CAUACUGAGUAAGAUCGGAAAUUUCGGUGUAAGGCCACGG-UAUGUGCGUCUACAUCUAGACUCAU845'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.Significantly reduced biofilm formation by 61.2%.8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/ABest CandidateN/AN/A
ABdb_1609 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su057GGGAGUCGACCGACCAGAA-UGGAUGUAUGGAACUUGCAGAUCUUAACUGCACGAAGCGU-UAUGUGCGUCUACAUCUAGACUCAU845'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.N/A8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_1610 357450142022The Ability of Nuclease-Resistant RNA Aptamer against Streptococcus suis Serotype 2, Strain P1/7 to Reduce Biofilm Formation In VitroR8-su15GGGAGUCGACCGACCAGAA-ACACGUUGCUGAAACAUACCGAGUAACAUAAAGCGGGUG-UAUGUGCGUCUACAUCUAGACUCAU835'-AGTAATACGACTCACTATAGGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'ssRNAStreptococcus Suis serotype 2, strain P1/7Whole cellIdentify aptamers that inhibit the biofilm formation of the S. suis target strain.N/A8Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ATherapeuticsWhole Cell-SELEX2'-Fluoro pyrimidines (2'-F-RNA)N/AN/AN/AN/AN/A
ABdb_1680 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K3CCGGAATTCCTAATACGACTC-CCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)36,70 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1681 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K4CCGGAATTCCTAATACGACTC-CCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1682 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K6CCGGAATTCCTAATACGACTC-GCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K6 has the highest binding affinity to S. aureus BPA-12.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)17,01 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1683 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K13CCGGAATTCCTAATACGACTC-CACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)60,69 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1684 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K15CCGGAATTCCTAATACGACTC-CAGGACAGTACTCTGGACGGCAATACGTATATACGTACGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20,90 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1685 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K20CCGGAATTCCTAATACGACTC-CCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStaphylococcus aureus (S. aureus) BPA-12Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1686 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K3CCGGAATTCCTAATACGACTC-CCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)28,06 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1687 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K4CCGGAATTCCTAATACGACTC-CCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1688 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K6CCGGAATTCCTAATACGACTC-GCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)31,99 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1689 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K13CCGGAATTCCTAATACGACTC-CACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K13 and S15K15 have high binding affinity to E. coli EPEC 4.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)5,21 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1690 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K15CCGGAATTCCTAATACGACTC-CAGGACAGTACTCTGGACGGCAATACGTATATACGTACGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEscherichia Coli (E. Coli) EPEC 4Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K13 and S15K15 have high binding affinity to E. coli EPEC 4.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)8,89 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1691 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K20CCGGAATTCCTAATACGACTC-CCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1692 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K3CCGGAATTCCTAATACGACTC-CCAGCAGCAAGGTGCGGTACCCGGGGATGCGGGCTTGCTG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K3 and S15K13 have a high binding affinity for S. agalactiae.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)6,84 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1693 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K4CCGGAATTCCTAATACGACTC-CCCGGGCCCACAGGGTACGCGTCTGCGGCTGGCCGGTCCC-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1694 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K6CCGGAATTCCTAATACGACTC-GCGGGACGGGGAGTGCGCTGGGCATGTGGGCGCCGGGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)20,79 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1695 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K13CCGGAATTCCTAATACGACTC-CACGCGCAGGCAGCCACCGACCAGGTGCTCGTATGGTTGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.S15K3 and S15K13 have a high binding affinity for S. agalactiae.15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)6,53 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1696 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K15CCGGAATTCCTAATACGACTC-CAGGACAGTACTCTGGACGGCAATACGTATATACGTACGG-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)45,36 nMDetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1697 357763862022A sequential toggle cell-SELEX DNA aptamer for targeting Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteriaS15K20CCGGAATTCCTAATACGACTC-CCGGCGCCACGACATGGGCGCTGCCGGTGTGGTCGCGGGA-TATTGAAAACGCGGCCGCGG815'-CCGGAATTCCTAATACGACTC-N40-TATTGAAAACGCGGCCGCGG-3'ssDNAStreptococcus AgalactiaeWhole cellIsolate polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli.N/A15Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ADetectionSequential Toggle Cell-SELEX (STC-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1698 358968422022Gold nanoparticles (AuNP)-based aptasensor for enteropathogenic Escherichia coli detectionS8-7CCGGAATTCCTAATACGACTCGGTTCCGGTAAGATTAGATCATAACGTATGGCTAGCGCCATATTGAAAACGCGGCCGCGG81N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellAn AuNP-based aptasensor was developed for the detection of enteropathogenic Escherichia coli.N/AN/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)17.18 nMBiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1699 358968422022Gold nanoparticles (AuNP)-based aptasensor for enteropathogenic Escherichia coli detectionS8-7.2GGTTCCGGTAAGATTAGATCATAACGTATGGCTAGCGCCATATTGAAAACGCGGCCGCGG60N/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellAn AuNP-based aptasensor was developed for the detection of enteropathogenic Escherichia coli.LOD value of AuNP-based aptasensor after overnight incubation with a value of 10(5) CFU/mL.N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)N/ABiosensorN/AN/AN/AN/AN/AN/AN/A
ABdb_1700 358968422022Gold nanoparticles (AuNP)-based aptasensor for enteropathogenic Escherichia coli detectionS10-5N/AN/AN/AssDNAEnteropathogenic Escherichia Coli (E. Coli) (EPEC K1.1)Whole cellAn AuNP-based aptasensor was developed for the detection of enteropathogenic Escherichia coli.LOD of AuNP-based aptasensor was achieved at overnight incubation with a value of 10(6) CFU/mL.N/AReal-Time Quantitative Polymerase Chain Reaction (RT-qPCR)34.14 nMBiosensorN/AN/AN/AN/ABest CandidateN/AN/A