AptBacterialDB

Determination of affinity method : Radiolabeled Binding assay

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0696 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA20GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 70.86 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0697 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA23GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 61.50 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A
ABdb_0698 255337622015Identification of Staphylococcus aureus infection by aptamers directly radiolabeled with technetium-99mSA34GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA88N/AssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cell99mTc-labelled aptamers for bacterial infection identification.EC50 value of 99mTc labelled aptamer was 72.42 nM and displayed a target/non-target ratio of 4.0±0.5.N/ARadiolabeled Binding assayN/ADiagnosticN/A5'-Amidation (NH₂-(CH2)6) and 5'-Radiolabelled (32P-labeled)N/ARadiolabeled aptamers with 99mTc were stable in 0.9% saline solution, in plasma, and in the presence of excess cysteine (50-, 500-, and 5000-fold), even for long period of 24hr.N/AN/AN/A