Determination of affinity method : Quartz Crystal Microbalance (QCM)

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0702 254675002015Pathogen detection in complex samples by quartz crystal microbalance sensor coupled to aptamer functionalized core-shell type magnetic separationAptamerCTGATGTGTGGGTAGGTGTCGTTGATTTCTTCTGGTGGGG40N/AssDNASalmonella Typhimurium (S. Typhimurium)Whole cellDeveloped an aptamer-based QCM biosensor for the determination of Salmonella in food samples.Showed specific detection of Salmonella cells at 100 CFU/mL from a milk sample and a linear response range from 100 to 4 × 10(4) CFU/mL cells.N/AQuartz Crystal Microbalance (QCM)3259 CFU mL−1BiosensorN/A5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0746 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB5CAGTCCAGGACAGATTCGCGAG-CCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATC-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.LOD was 7.9 × 10(3) CFU/mL and a linear range from 7.9 × 10(2) to 7.9 × 10(6) CFU/mL of S. typhimurium with less than 1 h.10Quartz Crystal Microbalance (QCM)58.5 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0747 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumA24CAGTCCAGGACAGATTCGCGAG-GCACAATCCACCTCTCACCGCACGCCACGCACTGCCTCTGTCCCG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)19.63 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0748 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB30AATCGCTGAATGAAATCCACGTG-GGAAGTGTGTGGGTGACCAGAGGTGTGGTGATGGGATTGTCGTA-CCTCGCGAATCTGTCCTGGACTG905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)51.02 nMBiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0749 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB31CAGTCCAGGACAGATTCGCGAG-CCGCACCGCCCTATCCTCGTGTCCCTGCCCAACAGCGCCGCCCGC-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0750 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumA37CAGTCCAGGACAGATTCGCGAG-GTGGGGCGGGAAAGTGTGTGTAGGTGGTGGCGTTGTCACGGCGGG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0751 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB16CAGTCCAGGACAGATTCGCGAG-GGGCTGTGTGAGAAGTGGGTGTGGTGGTGGATATGCAACGTTGTA-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0752 279792722016QCM-based aptamer selection and detection of Salmonella typhimuriumB21CAGTCCAGGACAGATTCGCGAG-GTCAACACGAGAGGAGGGGAGTGGAATCAGGATAGGTGTGTAGGG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-45N-CACGTGGATTTCATTCAGCGATT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers that bind to and detect S. typhimurium using a QCM-based aptasensor.N/A10Quartz Crystal Microbalance (QCM)N/ABiosensorQCM-based SELEX5'-Amidation (NH₂-(CH2)6)N/AN/AN/AN/AN/A
ABdb_1067 292421482018Whole-bacterium SELEX of DNA aptamers for rapid detection of E.coli O157:H7 using a QCM sensorS1CAGTCCAGGACAGATTCGCGAG-TGGTCGTGGTGAGGTGCGTGTATGGGTGGTGGATGAGTGTGTGGC-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellIdentify aptamers specifically bound to Escherichia coli O157:H7 and develop a QCM-based aptasensor for its detection.The limit of detection (LOD) was 1.46 × 10^3 CFU/mL, with a total detection time of 50 minutes.19Quartz Crystal Microbalance (QCM)10.30 nMBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_1068 292421482018Whole-bacterium SELEX of DNA aptamers for rapid detection of E.coli O157:H7 using a QCM sensorS2CAGTCCAGGACAGATTCGCGAG-GCGGGAATAGGATGCGGCTGGAAGGAGAGGTGTTGGTGGGTGGTG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellIdentify aptamers specifically bound to Escherichia coli O157:H7 and develop a QCM-based aptasensor for its detection.N/A19Quartz Crystal Microbalance (QCM)N/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1069 292421482018Whole-bacterium SELEX of DNA aptamers for rapid detection of E.coli O157:H7 using a QCM sensorS3CAGTCCAGGACAGATTCGCGAG-GTGCGGTGACGTGAGGGGGAGAGGCGTTGGTGTAGGCTGTTGGTG-CACGTGGATTTCATTCAGCGATT905'-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 43888)Whole cellIdentify aptamers specifically bound to Escherichia coli O157:H7 and develop a QCM-based aptasensor for its detection.N/A19Quartz Crystal Microbalance (QCM)N/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A