AptBacterialDB

Determination of affinity method : Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)

Total records found: 13

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0984 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-03CATATCCGCGTCGCTGCGCTCAGACCCACCACCACGCACC405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.STC-03 showed better affinity to E. aerogenes, K. pneumoniae, C. freundii, and B. subtilis.18Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)27.2 nM (for E. coli), 11.9 nM (for E. aerogenes), 9.22 nM (for K. pneumoniae), 15.9 nM (for C. freundii), 9.97 nM (for B. subtilis), and 16.4 nM (for S. epidermidis)DetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0993 282725542017Broadly reactive aptamers targeting bacteria belonging to different genera using a sequential toggle cell-SELEXSTC-12GGACCGCAGGTGCACTGGGCGACGTCTCTGGGTGTGGTGT405'-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571), Enterobacter aerogenes (E. aerogenes) (KCTC 2190), Klebsiella pneumoniae (K. pneumoniae) (KCTC 2208), Citrobacter freundii (C. freundii) (KCTC 2006), Bacillus Subtilis (B. Subtilis) (KCTC 1022), and Staphylococcus Epidermidis (S. Epidermidis) (KCTC 1917)Whole cellIdentify aptamers with broad affinity for bacteria across different genera.STC-12 showed better affinity to E. coli and S. epidermidis.18Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)38.5 nM (for E. coli), 33.7 nM (for E. aerogenes), 16.3 nM (for K. pneumoniae), 25.6 nM (for C. freundii), 18.9 nM (for B. subtilis), and 13.7 nM (for S. epidermidis)DetectionSequential Toggle Cell-SELEX (STC-SELEX)3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1357 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20–5GCAATGGTACGGTACTTCC-ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)3.9 ± 0.6 nMDetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1358 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20–7GCAATGGTACGGTACTTCC-CGCAATACCAAAGTGGCGAGAGCGCTGTCTTGAGTGAGTGGTTGG-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)8 ± 0.9 nMDetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1359 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20–10GCAATGGTACGGTACTTCC-TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)10.1 ± 1.7 nMDetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1360 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-1GCAATGGTACGGTACTTCC-GATTAGCTACATTTGGTTGTTTACCGCTCTGCTTTCTATTATTT-CAAAAGTGCACGCTACTTTGCTAA875'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1361 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-2GCAATGGTACGGTACTTCC-TGTTAGTGTTTAAGGCCCAAAGTCGGTTCATCAGTACATTCCTCG-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1362 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-3GCAATGGTACGGTACTTCC-TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA-CAAAAGTGCACGCTACTTTGCTAA855'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1363 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-4GCAATGGTACGGTACTTCC-GATTGTGGTGGGGCCTCGAGATACCTGCGACCGGCATACTTGAAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1364 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-6GCAATGGTACGGTACTTCC-TTGCCCGTACACTGTCATCCTCGGCTTATAGCCATTATTGAAATT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1365 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-8GCAATGGTACGGTACTTCC-GGGCCTATACAGGCTTTTACTTCTGAGTTTGGTAGTTTCTTCGGA-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1366 318379672020Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method20-9GCAATGGTACGGTACTTCC-GCGAGGGCCAACGGTGGTTACGTCGCTACGGCGCTACTGGTTGAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAEscherichia Coli (E. Coli) (KCTC 2571)Whole cellIsolate aptamers against bacterial cells.Aptamer selection was much faster compared to SELEX-based aptamer isolation.20Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)N/ADetectionCentrifugation-based Partitioning Method3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1970 233816902013Selection of aptamers against inactive Vibrio alginolyticus and application in a qualitative detection assayPool of enriched aptamersN/AN/A5'-TCAGTCGCTTCGCCGTCTCCTTC-N35-GCACAAGAGGGAGACCCCAGAGGG-3'ssDNAVibrio AlginolyticusWhole cell (Inactivated)Identify aptamers against and qualitatively detect inactive Vibrio alginolyticus using PCR.V. alginolyticus could be detected at 100 cells/ml.15Micro-Fluorospectrophotometer (Fluorescence Spectroscopy)27.5 ± 9.2 nMDetectionN/AN/AN/AN/AN/AN/AN/A