AptBacterialDB

Determination of affinity method : Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0080 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 4RATCCGTCACACCTGCTCT-ACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA-TGGTGTTGGCTCCCGTAT735'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.Detect as few as 30 live, unlabeled E. coli per ml using FRET assay.5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0081 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 3RATCCGTCACACCTGCTCT-GTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCG-TGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.N/A5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/AN/AN/AN/A
ABdb_0082 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 8FATACGGGAGCCAACACCA-CGACTAACACGACCGTTGGGGGGGGCTCGCGCGGGC-AGAGCAGGTGTGACGGAT725'-ATACGGGAGCCAACACCA-N36-AGAGCAGGTGTGACGGAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.N/A5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/AN/AN/AN/A