AptBacterialDB

Determination of affinity method : Fluorescence Microplate Reader (Fluorescence Spectroscopy)

Total records found: 10

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0103 217439202011Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties1–PAGCGCGGATCCCGCGC-GATGTGGGTGTAGTTGGAGGGTAAACGTT-CGCGCGAAGCTTGCG595'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNABacillus Anthracis (BA)Protective antigen (PA) of anthrax toxinDetection of PA based on the reduction in the fluorescence emission according to the formation of the aptamer-PA ternary complex.The limit of PA detection using this system was determined to be approximately 1 nM.8Fluorescence Microplate Reader (Fluorescence Spectroscopy)8.53 nMBiosensorSELEXN/AN/AN/AN/AN/AKorea Pat., KR101152354B1 (2012-06-13)
ABdb_0104 217439202011Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties2–PAGCGCGGATCCCGCGC-CAGACCGTAAGGGATGCCGCCTAAACACC-CGCGCGAAGCTTGCG595'-GCGCGGATCCCGCGC-N30-CGCGCGAAGCTTGCG-3'ssDNABacillus Anthracis (BA)Protective antigen (PA) of anthrax toxinDetection of PA based on the reduction in the fluorescence emission according to the formation of the aptamer-PA ternary complex.The limit of PA detection using this system was determined to be approximately 1 nM.8Fluorescence Microplate Reader (Fluorescence Spectroscopy)1.51 nMBiosensorSELEXN/AN/AN/AN/AN/AKorea Pat., KR101152354B1 (2012-06-13)
ABdb_1599 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE1TAGGGAAGAGAAGGACATATGA-CGATTACCGGAGCTGTGTCACCGGGCGCCAGTTGATGTGG-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1600 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE2TAGGGAAGAGAAGGACATATGA-TATGATGGGAGTAACGATTGTCCGACATGGTACCACCCCA-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1601 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE3TAGGGAAGAGAAGGACATATGA-GGTGCACCTCTCGCCCGAATCAGATCCCCACGGCGCCCCCTA-TTGACTAGTACATGACCACTTGA875'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1602 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE4TAGGGAAGAGAAGGACATATGA-ATCGGCTACGAGGTCCCGACTGCCGGACTGGCCCTTGGTC-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1603 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE5TAGGGAAGAGAAGGACATATGA-ACACCGCCACGATGCAACTGCCAAACGCACCGTACGCCTG-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1604 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE6TAGGGAAGAGAAGGACATATGA-CGAGAGGCCGCTGGGTCTAGACGTCCGGGTGCCACTCGCG-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1605 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE7TAGGGAAGAGAAGGACATATGA-TAACGCCTGTAGTACTCCACCTTAATCCGTTGCCAGGAAT-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.N/A7Fluorescence Microplate Reader (Fluorescence Spectroscopy)N/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1606 https://doi.org/10.1016/j.snb.2020.1291002021Naked eye colorimetric detection of Escherichia coli using aptamer conjugated graphene oxide enclosed Gold nanoparticlesE8TAGGGAAGAGAAGGACATATGA-CATTCTGCACCGCCATCAGTCTTTCTTCGATACCGCGTTT-TTGACTAGTACATGACCACTTGA855'-TAGGGAAGAGAAGGACATATGA-46N-TTGACTAGTACATGACCACTTGA-3'ssDNAEscherichia Coli (E. Coli) (MTCC no.1698)Whole cellIdentify an aptamer against E.coli and develop a detection platform using gold nanoparticles and graphene oxide with aptamer conjugation.The limit of detection observed visually was 10(2) cells/mL with GO coating and 10(3) cells/mL without GO. The detection limit in real-time coconut water samples was also 10(2) cells/mL.7Fluorescence Microplate Reader (Fluorescence Spectroscopy)15.90 ± 3.07 nMBiosensorWhole Cell-SELEX5'-FAM Labeled, 5'-Thiolated and 5'-Amidation (NH₂)N/AN/ABest CandidateN/AN/A