AptBacterialDB

Determination of affinity method : Flow Cytometry

Total records found: 406

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0042 188033932008Selection of aptamers against live bacterial cellshemag1PAGCAGCACAGAGGTCAGATG-TAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGTG-CCTATGCGTGCTACCGTGAA785'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).The number of aptamer molecules binding to each cell (on average, ∼164 ± 47 aptamer molecules per cell).8Flow Cytometry13 ± 3 nMDetectionSELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0043 188033932008Selection of aptamers against live bacterial cellshemag1TAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGATG395'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0044 188033932008Selection of aptamers against live bacterial cellsmag1TGAGCCCCACTAAAGTTGCAATCATGTCGTCAGCTTTGGG405'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0045 188033932008Selection of aptamers against live bacterial cellshemag3CGTCGCGGCATATTTCCAGTGGAACGGTTACGATATGTTG405'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0046 188033932008Selection of aptamers against live bacterial cellsmag1PAGCAGCACAGAGGTCAGATG-TGAGCCCCAGTAAAGTTGCAATCATGTCGTCAGCTTTGGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0047 188033932008Selection of aptamers against live bacterial cellshemag3PAGCAGCACAGAGGTCAGATG-TCGTCGCGGCATATTTCCAGTGGAACGGTTACGATATGTTG-CCTATGCGTGCTACCGTGAA815'-AGCAGCACAGAGGTCAGATG-40N-CCTATGCGTGCTACCGTGAA-3'ssDNALactobacillum Acidophilius (ATCC 4356)Surface protein (S-layer protein)Identify aptamers that bind only specifically to cells with S-layers composed of similar S-proteins (L. acidophilus 4355, 4356, and 4357) and not to other cells (E. coli, S. bovis, and S. cerevisiae).N/A8Flow CytometryN/ADetectionSELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0049 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA20GCAATGGTACGGTACTTCC-GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellIdentify aptamers that bind to S. aureus strains, including 8325-4, 196, and 04018, and develop a probe to distinguish them from S. epidermidis, Streptococcus, and E. coli.EC50 = 70.86 ± 39.22nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0050 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA23GCAATGGTACGGTACTTCC-GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 = 61.50 ± 22.43nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0051 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA31GCAATGGTACGGTACTTCC-TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50=82.86 ± 33.20nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0052 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA34GCAATGGTACGGTACTTCC-CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 =72.42 ± 35.23nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0053 194980772009Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusSA43GCAATGGTACGGTACTTCC-TCGGCACGTTCTCAGTAGCGCTCGCTGGTCATCCCACAGCTACGT-CAAAAGTGCACGCTACTTTGCTAA885'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStaphylococcus aureus (S. aureus)Whole cellRecognition of different S. aureus strains and mixed aptamer probes distinguishes S. aureus strains, including 8325-4, 196 and 04018, from S. epidermidis, Streptococcus and E. coli.EC50 = 210.70 ± 135.91nM and with Multiple aptamers: 479.98 ± 209.94nM.5Flow CytometryN/ADetectionSubtractive SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0093 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A24PAGCAGCACAGAGGTCAGATG-GGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGG-CCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.20A24P forms a branched structure with high affinity for the target cell mixture (gated fluorescence intensity above library of 64%).20Flow Cytometry9.1 ± 0.8 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0094 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes15A3PTTCACGGTAGCACGCATAGG-GACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGG-CATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.Show a gated fluorescence above the background of 48%.20Flow Cytometry9.6 ± 0.3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0095 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A1CAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCC40N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.72% gated fluorescence intensity above the library.20Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0096 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A1PTTCACGGTAGCACGCATAGG-CAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCC-CATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.72% gated fluorescence intensity above the library.20Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0097 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A8CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC40N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.20A8 binding to the target GAS cells yielded 72% gated fluorescence above background.20Flow Cytometry4 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0098 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A8PAGCAGCACAGAGGTCAGATG-CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC-CCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.20A8P binding to the target GAS cells yielded 68% gated fluorescence above background.20Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0099 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A9CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG39N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.65% gated fluorescence intensity above the library.20Flow Cytometry9 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0100 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A9PAGCAGCACAGAGGTCAGATG-CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG-CCTATGCGTGCTACCGTGAA79N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.66% gated fluorescence intensity above the library.20Flow Cytometry9.1 ± 0.6 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0101 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A12PTTCACGGTAGCACGCATAGG-GCCCGACACTCGTCCACCCGATACCTCTCATGTGTCCC-CATCTGACCTCTGTGCTGCT78N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.N/A20Flow Cytometry25 ± 3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0102 215041822011DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes20A14PAGCAGCACAGAGGTCAGATG-GGCATGGGGAAGAGAAAGCGGGATAACTTCGTTACCGGGC-CCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesWhole cellIdentify aptamers against the various M-types of S. pyogenes.N/A20Flow Cytometry17 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0105 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF23ATACCAGCTTATTCAATT-CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.F23 showed great specificity to P. aeruginosa and no cross-reactivity with other bacterial species.15Flow Cytometry17.27 ± 5.00 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0106 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF12ATACCAGCTTATTCAATT-CCCCCGTTGCTTTCGCTTTTCCTTTTGCTTTTGTTCGCTTCGTCCCTGCTTCCTTTCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0107 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF8ATACCAGCTTATTCAATT-CCGTGGCGTTCTGTTTGTTCGTTGTTTTTTGGTTTTTCCTTCTTTTTTTGTTTTTGGTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0108 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF1ATACCAGCTTATTCAATT-GGCGCGCGTTGTGTGGTTTGGCTTTTTTCGTTTGTTTTTCTTGGGTGCTTGTCTTCGTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0109 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF20ATACCAGCTTATTCAATT-CCCTCGCCGTACGTTCTCCGTTTTGTCAGGTGTTGTTTTTTCCGTCCTCTTCTCGTGTGC-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0110 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF4ATACCAGCTTATTCAATT-CCACCGATTCCCTTCGATCGTATTCGTGTTGCTCTCGTGTTGTAATGCGTCCTGTGCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0111 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF6ATACCAGCTTATTCAATT-GGCACCGGCTTGTCTGTTTCTCTTGGTTCGCCGCTGGACTTTGTTAGCTCCCTCCGCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0112 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF7ATACCAGCTTATTCAATT-CCCCACAACCTGACCTTTCTATTCCGTTCCCCTTTTTTCAGCTTCTTTCCGGCCTCTGTT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0113 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF18ATACCAGCTTATTCAATT-CCACCACCGGGTTGTTTTTCCTGCAGGCCTTTATCGTTTTCCGCGCTCGATTCGGTCATG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0114 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF5ATACCAGCTTATTCAATT-CCACCGTGCTTTATATTACCCGTCCACCCTTCGGTCTTTCTCCCCGTGTCGCTCCGCTTT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0115 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF21ATACCAGCTTATTCAATT-CCACCGGGCCTCGTATTCGTACTGTTTGATTCTTTGGCTTTGTAGCGGACGTACTGGGTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0116 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF2ATACCAGCTTATTCAATT-CCCCCGCGCCCGTCTCTCTCAATCTCCGCATTCTTTAGTTCTCATCCTCCCTGTGTGTTT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0117 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF24ATACCAGCTTATTCAATT-GACACCCGGAGTAATTCCGTTTTAAGCGTCTTTCATGTGTTCCCTTTCGTTCCTCCCTTG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0118 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF15ATACCAGCTTATTCAATT-CCCAAGGTCGAGTTCCTAGTCTTACCGTATTTCACTTTCCTGGTTCTTACCTCCCCCTCA-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0119 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF22ATACCAGCTTATTCAATT-CGGCGCGAGTCGACTGCACAGGGTCCTATTGCTTATGGTCATGGTTTCGTTTCATCCTCG-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0120 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF16ATACCAGCTTATTCAATT-CCCGCGATATGACCATCCAGCTGCTCCTCTGTTATTGTTGGTTTTCCTGTTTTCCTTTGT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0121 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF17ATACCAGCTTATTCAATT-CCCCAGTGCTCAGCTTCTCCTCCGTCTCATTCTTCTGTAGGATCGTTCTGTTTTGGTACT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow Cytometry57.63 ± 11.64 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0122 209364922011Utility of aptamer-fluorescence in situ hybridization for rapid detection of Pseudomonas aeruginosaF10ATACCAGCTTATTCAATT-CCCACCGTCCTCGTGCTTAGTCTTTATGTTTCGTCCTTTTCTTTCTTCGTTCTGTCGCCT-AGATAGTAAGTGCAATCT965'-ATACCAGCTTATTCAATT-60N-AGATAGTAAGTGCAATCT-3'ssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellIdentify aptamers that specifically bind to and develop a rapid FISH assay to detect P. aeruginosa.N/A15Flow CytometryN/ADetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0124 223100302012Development of RNA aptamers for detection of Salmonella EnteritidisS25GGGUUCACUGCAGACUUGACGAAGCUU-GAGAGAUGCCCCCUGAUGTGCAUUCUUGUUGUGUUGCGGC-AAUGGAUCCACAUCTACGAAUUC905'-GGGUUCACUGCAGACUUGACGAAGCUU-N40-AAUGGAUCCACAUCUACGAAUUC-3'ssRNASalmonella Enteritidis (S. Enteritidis)Whole cellIdentify an aptamer that binds to the outer membrane protein C and specifically recognizes S. enteritidis without any cross-reactivity to other Salmonella serovars.Fluorescence intensity of S. Enteritidis enhanced as the concentration of the aptamer S25 increased from 5 to 30 μg.10Flow CytometryN/ADetectionMagnetic Bead (MB)-based SELEX5'-Fluoro-labeled with Fluorescein-maleimideN/AN/AN/AN/AN/A
ABdb_0125 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA3PATAGGAGTCACGACGACCAGAA-TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.Showed a high binding affinity of 76% for V. parahemolyticus and a low apparent binding affinity of 4% for other bacteria.9Flow Cytometry16.88 ± 1.92DetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0126 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA1TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG405'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A1 was 64%.9Flow Cytometry22.92 ± 4.72 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0127 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA1PATAGGAGTCACGACGACCAGAA-TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A1P was 67%.9Flow Cytometry21.45 ± 2.62 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0128 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA3TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT405'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A3 was 75%.9Flow Cytometry24.03 ± 5.18 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0129 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA17PATAGGAGTCACGACGACCAGAA-AGGCCGGCCCCTCTGAACTAGATGCAGGGGAGGCGAGCGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0130 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA18PATAGGAGTCACGACGACCAGAA-GCGGGCAGCTCCACCGGTAGGCTCCGAGTCATCACGGTCG-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.The gated fluorescence intensity above background for A18P was 62%.9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0131 224802092012Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticusA21PATAGGAGTCACGACGACCAGAA-TTTTGACGAAGTCAGTGCGTCGAAGCGCAAGCATTACAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify aptamers against V. parahemolyticus and develop molecular probes for its detection in food and environmental samples.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0157 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-1CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTG465'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0158 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-2ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCAT405'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0159 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-3GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGC395'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).Successfully detect S. typhimurium down to 600 CFU/mL (equivalent to 18 live cells in 30 μL of assay volume) and distinguish it from other Salmonella species, including S. enteritidis and S. choleraesuis.12Flow Cytometry25 nMDetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/ABest CandidateN/AN/A
ABdb_0160 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-4CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0161 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-5GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTCGCATAGGTAGTCCAGAAGCC615'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0162 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-6CTCCTCTGACTGTAACCACG-GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGCC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0163 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-7CTCCTCTGACTGTAACCACG-GTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGTC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0164 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-8CTCCTCTGACTGTAACCACG-GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0165 230754172012Aptamer-based viability impedimetric sensor for bacteriaSTYP-9CTCCTCTGACTGTAACCACG-ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCATC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cellIdentify aptamers against viable S. typhimurium and develop an aptamer-based impedimetric sensor for bacterial viability (AptaVISens-B).N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-Thiolated (HS-(CH2)6)N/AN/AN/AN/AN/A
ABdb_0197 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-3 (80nt)CTCCTCTGACTGTAACCACG-TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACT-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0198 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-3 (60nt)TCGGCAACAAGGTCACCCGGAGAAGATCGGTGGTCAAACTGCATAGGTAGTCCAGAAGCC605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry7.8 ± 6.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0199 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-6 (79nt)CTCCTCTGACTGTAACCACG-TAACGTACCAAAATGTTGGATTGGATGTTGTACTGGGTT-GCATAGGTAGTCCAGAAGCC795'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry53 ± 7 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0200 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-6_54TACCAAAATGTTGGATTGGATGTTGTACTGGGTTGCATAGGTAGTCCAGAAGCC545'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry6.3 ± 0.58 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0201 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-20_80CTCCTCTGACTGTAACCACG-CACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry28 ± 3.8 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0202 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-20_60CTCCTCTGACTGTAACCACGCACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis and 43 ± 4% (SE-20, 60nt) in S. enteritidis.12Flow Cytometry7.1 ± 0.62 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0203 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-22_80CTCCTCTGACTGTAACCACG-TATACGCGCTTGCCCCTTAGTCATACGAACTGATTCAATC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry30 ± 4.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0204 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-22_60CTCCTCTGACTGTAACCACGTATACGCGCTTGCCCCTTAGTCATACGAACTGATTCAATC605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry5.3 ± 0.7 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0205 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-11_60CTCCTCTGACTGTAACCACGAACGATTCAAGAACTGTTGGTTGTCGGCTTATTTTCGCCA605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry6.9 ± 0.4 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0206 233875112013Development of bacteriostatic DNA aptamers for salmonellaSE-34_80CTCCTCTGACTGTAACCACG-TGCCGCTAAACGCCGGCTCATCGTTATGCTTTTCATTGCA-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Enteritidis (S. Enteritidis)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).73 ± 15% inhibition ratio for S. enteritidis.12Flow Cytometry56 ± 7.1 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0207 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-1_40GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGC395'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0208 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-6_60GCCTCTAAGGCTCACCTTGAAGCGCCCGGACTAACCTGCTCGCATAGGTAGTCCAGAAGCC615'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0209 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-12_80CTCCTCTGACTGTAACCACG-GTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGTC-GCATAGGTAGTCCAGAAGCC805'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0210 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-12_60CTCCTCTGACTGTAACCACGGTGGTTTGATCACTATTGGGCCTTTGTGATGTCGGTAGT595'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium and 62 ± 12% (ST-12, 60nt) in S. typhimurium.12Flow Cytometry4.5 ± 0.4 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0211 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-20_80CTCCTCTGACTGTAACCACG-ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCATC-GCATAGGTAGTCCAGAAGCC815'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0212 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-20_40ATTGTACACCATGTGCAGAGATTTTCGGCACGAGGATCAT405'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow CytometryN/ATherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0213 233875112013Development of bacteriostatic DNA aptamers for salmonellaST-33_60CTCCTCTGACTGTAACCACGGTGGGAGAGATGCTATACAATCTTGTAAGGCGATGGACCG605'-CTCCTCTGACTGTAACCACG-N40-GCATAGGTAGTCCAGAAGCC-3'ssDNASalmonella Typhimurium (S. Typhimurium)Whole cell (bind to antibiotics resistant Salmonella)Identify an aptamer against the whole cell and induce strong depolarization of the bacterial cell membrane (Inhibition of growth of S. enteritidis and S. typhimurium in bacterial cultures and a decrease in their membrane potential).75 ± 15% inhibition ratio for S. typhimurium.12Flow Cytometry51.0 ± 4.3 nMTherapeuticsWhole Cell-SELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_0214 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST2PATAGGAGTCACGACGACCAGAA-AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.The limit of detection (LOD) was 25 cfu/mL, and the percentage gated fluorescence intensity above library background was 20% when ST9P was incubated with L. monocytogenes cells and 72% when incubated with S. typhimurium.9Flow Cytometry6.33 ± 0.58 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0215 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST2AGTAATGCCCGGTAGTTATTCAAAGATGAGTAGGAAAAGA405'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 82 ± 2.34% for ST2.9Flow Cytometry16.34 ± 0.45 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0216 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST3PATAGGAGTCACGACGACCAGAA-TTTCGGCTAAGGACGGGTGAAATACATTTAATAGGGTGGA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.N/A9Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0217 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST7PATAGGAGTCACGACGACCAGAA-TAAGTACAGGACTGGAGTATTAGCGGGGTCCATGCAAGGC-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.N/A9Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0218 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST9AATCAATAGAAGACAAAGTCCGAAACAGGTGTGACGGTAA405'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 72 ± 1.57% for ST9.9Flow Cytometry9.45 ± 0.63 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0219 234735452013Selection and characterization of aptamers against Salmonella typhimurium using whole-bacterium SELEXST9PATAGGAGTCACGACGACCAGAA-AATCAATAGAAGACAAAGTCCGAAACAGGTGTGACGGTAA-TATGTGCGTCTACCTCTTGACTAAT875'-ATAGGAGTCACGACGACCAGAA-40N-TATGTGCGTCTACCTCTTGACTAAT-3'ssDNASalmonella Typhimurium (S. Typhimurium)Outer membrane protein (Lipopolysaccharide (LPS) or lipoprotein) of whole cellIdentify an aptamer that binds to and develop a fluorescent bioassay based on aptamer-MNPs conjugate to detect S. typhimurium.Gated fluorescence intensity above background remained at 66 ± 1.45% for ST9P.9Flow Cytometry11.14 ± 0.12 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0238 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA1ATCCAGACGTGACGCAGC-ATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTG-TGGACACGGTGGCTTAGTA755'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA1 and DTMRSA3 showed the best specificity for MRSA.17Flow Cytometry1.6 ± 0.5 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/ABest CandidateN/AN/A
ABdb_0239 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA2ATCCAGAGTGACGCAGCA-CGACACGTTAGGTTGGTTAGGTTGGTTAGTTTCTTG-TGGACACGGTGGCTTA705'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA2 and DTMRSA4 bound to all three types of clinical bacterial strains.17Flow Cytometry2.0 ± 0.6 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0240 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA3ATCCAGAGTGACGCAGCA-GTAGATGGTTTGGTTGGTGTGGTTTCCTACTGATGTTGGG-TGGACACGGTGGCTTAGTA775'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA1 and DTMRSA3 showed the best specificity for MRSA.17Flow Cytometry1.3 ± 0.5 × 10(2) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0241 254361842013Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamersDTMRSA4ATCCAGAGTGACGCAGCA-TTATGGGGTGTGGTGGGGGGTTAATGCGTTGGTTATCCG-TGTGGACACGGTGGCTTA755'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMethicillin-resistant Staphylococcus aureus (MRSA)Whole cellIdentify aptamers against MRSA and conjugate them to AuNPs for their detection.DTMRSA2 and DTMRSA4 bound to all three types of clinical bacterial strains.17Flow Cytometry9.5 ± 2 × 10(1) nMDetectionWhole Cell-SELEX5'-Biotinylated or FITC Labeled or ThiolatedN/AN/AN/AN/AN/A
ABdb_0250 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-17AGTATACGTATTACCTGCAGC-GAGGGAAGAAGGGCCAGCACAGATCAGATCAATCGCTCCG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.Lbi-17 showed maximum binding of 78.7 ± 12.38% with an LOD of the combined AMC-qPCR method of 1.8 log10 CFU/500μl L.monocytogenes and ranged from 26% to 77%.6Flow Cytometry35.7 ± 8.02 μMBiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0251 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-16AGTATACGTATTACCTGCAGC-AAATACTTTAGATCTAAGAGTGTTTCGAAAAGACAACAGA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0252 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-118AGTATACGTATTACCTGCAGC-TTGAATTAATAGATTAGTATACTTGGGAATCGTCCTAATA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0253 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-200AGTATACGTATTACCTGCAGC-AGGAAGACAAATTCCGCCAAAAAGTGGATATAACCAATAA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0254 239423782013Nucleic acid aptamers for capture and detection of Listeria sppLbi-203AGTATACGTATTACCTGCAGC-ATAGAGTAGAAGCTACACTACGTAATCACAGACAGATCCA-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify aptamers against Listeria spp. and develop aptamer-mediated magnetic capture (AMC) for detecting these bacteria.N/A6Flow CytometryN/ABiosensorWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0331 234946202013Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sortingS8-7GTATACGTATTACCTGCAGC-CTGATGTGTGGGTAGGTGTCGTTGATTTCTTCTGGTGGGG-CGATATCTCGGAGATCTTGC805'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNASalmonella Typhimurium (S. Typhimurium) (Strain S913)Whole cellIdentifying aptamers selected by whole-cell SELEX and developing a qPCR-based capture-detection platform for Salmonella Typhimurium.N/A10Flow Cytometry1.73±0.54 μMDetectionWhole Cell-SELEX5'-BiotinylatedN/AN/ABest CandidateN/AN/A
ABdb_0332 234946202013Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sortingS8-46GTATACGTATTACCTGCAGC-CTTGGGCGGTTGGTGTGATGGGCTTTTTTCGTTGGGCCGG-CGATATCTCGGAGATCTTGC805'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNASalmonella Typhimurium (S. Typhimurium) (Strain S913)Whole cellIdentifying aptamers selected by whole-cell SELEX and developing a qPCR-based capture-detection platform for Salmonella Typhimurium.Achieved a Lower Limit of Detection (LOD) of 10(2)-10(3) CFU in a 290-μl sample, and the mean capture efficiency ranged from 3.6% to 12.6%.10Flow Cytometry0.74±0.20 μMDetectionWhole Cell-SELEX5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0396 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G6-25GGATCTGGTTAGTGAAGAGGGGGGAGGGGGGGTTGGGCTGTGGGGCGGTTAGTCGT56N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A6Flow CytometryN/ADetectionIn Silico Maturation (ISM)5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_0397 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-5ATACATCTTAAGTCTCGTGGGGGGAGGGGGGGTTGGTGGGCTTT44N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)β-structure of membrane associated proteinImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.Colorimetric assay demonstrated detection of S. mutans cells at a concentration range of 1 × 10(5)-10(8) CFU/mL.11Flow Cytometry33 nMDetectionIn Silico Maturation (ISM)5'-ThiolatedN/AN/ABest CandidateN/AN/A
ABdb_0398 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-6ATACTATCGCATTCCTTCCGAGGGGGGAGGGGGGGGTGGGGGTCGGT47N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)β-structure of membrane associated proteinImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.Showed the highest binding signals to S. mutans with a 16-fold increase over SMa#3-6-P1.11Flow Cytometry33 nMDetectionIn Silico Maturation (ISM)5'-Fluorescein LabeledN/AN/ABest CandidateN/AN/A
ABdb_0399 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#3-6-P1ATACCAGCTTATTCAATTGGGGGGAGGGGGGGTTGGGCTGTGGGGCGGTTAGCCGC56N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A3Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0400 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-20CTACGTCTAGATTCCAGTCGAGGGGGGAGGGGGGGTTTTGGATCGGT47N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A11Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0401 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G10-11CAACCTGCTTATCTAAGGGGGGGAGGGGGGGTTGTGGGTAGGT43N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A10Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0402 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G2-1CTAGCAGTTCATTCAATAGGGGGGACGGGGGGTTAGGCTAGACTATGTCTAATCA55N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A2Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0403 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G5-5ATACACGTAAGTACCATTGGGCGGGGGGGGTGTTGTTTTCTACTATGTGCAATCG55N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A5Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0404 240189052014Simultaneous improvement of specificity and affinity of aptamers against Streptococcus mutans by in silico maturation for biosensor developmentSMa#G11-8ACACCACTTAATTCCATGCGAGGGGGGAGGGGGGGTTCGGGGACGGC47N/AssDNAStreptococcus Mutans (ATCC 25175 and JCM 5175)Whole cellImprovement of affinity and specificity of aptamers against S. mutans using ISM and detection by aptamer-immobilised gold colloids.N/A11Flow CytometryN/ADetectionIn Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0435 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-2AGTATACGTATTACCTGCAGC-TGGGGGGTGGTTGGGGGTAGTATATCGGGTCAGTGGTGCG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0436 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-103AGTATACGTATTACCTGCAGC-GGGAGGCGTGAAGTGTGATGGTGTGGGGGGTAGTGGCCGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0437 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-109AGTATACGTATTACCTGCAGC-CCGCTCAACAAGGTGCTACAAAGATCCTGTGTCCCTTGGC-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0438 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-116AGTATACGTATTACCTGCAGC-TACTCGTTATTTCGTAGCACTTTTCCCCACCACCTTGGTG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.LM6-116 showed strong genus-specific binding affinity when screened against five different species within the Listeria genus.6Flow Cytometry74.4 ±52.69 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0439 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-118AGTATACGTATTACCTGCAGC-CTGGTATAGACGTTATTCGTCCGCTCTTGTATCCTTGTGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0440 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM6-121AGTATACGTATTACCTGCAGC-TGTCGGTGGGGGGTGGCTTGAGTACGTGACGTGGTGTGGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0441 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM12-2AGTATACGTATTACCTGCAGC-CCATTGTTCTATCGCTAGCTCGCTCTGGCCAGCTCCTTGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0442 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM12-6AGTATACGTATTACCTGCAGC-TCTGTGTTCCGTTTTCGATTCTTACTGTGTTTTCGGGTGC-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow Cytometry106.4 ±43.91 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0443 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM12-13AGTATACGTATTACCTGCAGC-TGGGGGGGGGGGGAGGGGTCGGTGAGCGTGGGAGGAGGAG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0444 248577732014Selection and characterization of DNA aptamers specific for Listeria speciesLM12-74AGTATACGTATTACCTGCAGC-CCGACCTTCTATCCTTCCAGTTTTTTGTAAACTCTTCTGG-CGATATCTCGGAGATCTTGC815'-AGTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNAListeria Monocytogenes (ATCC 19115)Whole cellIdentify an aptamer against Listeria spp. at different growth phases.N/A6Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0446 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmA_Apt1CACAGCGGGACAGUUUAGC-AUUAGCUAUCUUCCACGAUUCGAUCAGGCAGUACGUCGU-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer mA_Apt1 (~40% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/ABest CandidateN/AN/A
ABdb_0447 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmB_Apt23CACAGCGGGACAGUUUAGC-ACAGUUAUCCGACUGCUAUUCGAUCAGGCAGUACGUAGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells. A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer mB_Apt23 (~20% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0448 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmC_Apt41CACAGCGGGACAGUUUAGC-CAGGCUGUUCUGACGCAUAAGGAAUGCGCUGUUGCAGAG-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.mC_Apt41 neutralized 0.007 pmole of Stx2 (≤60% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0449 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinsmD_Apt46CACAGCGGGACAGUUUAGC-UUGGUCCUGCUUUGGAUAGUCGCGAAAGGGGUGCCACUG-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0450 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspA_Apt1CACAGCGGGACAGUUUAGC-ACAGUUAUCCGACUGCUAUUCGAUCAGGCAGUACGUAGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Inhibited Stx2 binding weakly in approximately 50% of HeLa cells.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0451 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspB_Apt14CACAGCGGGACAGUUUAGC-ACCGAGCGGUUUUACGUCUCAAGUAGUAUCCCGUUUUGC-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Did not inhibit Stx2 binding.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0452 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspC_Apt22CACAGCGGGACAGUUUAGC-AUUAGCUAUCUUCCACGAUUCGAUCAGGCAGUACGUCGU-GGUAGGUGGGUGCGUCUAAA785'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.A limited neutralization of Stx2-mediated cytotoxicity and death of HeLa cells was observed with aptamer pC_Apt22 (≤60% cell survival).5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0453 248395532014Selective Evolution of Ligands by Exponential Enrichment to Identify RNA Aptamers against Shiga ToxinspD_Apt25CACAGCGGGACAGUUUAGC-UUGCCAUCCUGUACUAUGCUCUAUCGGGCGGUUUAGUGAUCCUUCGUCCAACUAUC-GGUAGGUGGGUGCGUCUAAA955'-CACAGCGGGACAGTTTAGC-N56-GGTAGGTGGGTGCGTCTAAA-3'ssRNAEscherichia Coli (E. Coli)Shiga toxin 2 (Stx2)Identify RNA aptamers against Stx1 and Stx2.Did not inhibit Stx2 binding.5Flow CytometryN/ADetectionParallel m-SELEX (Membrane) and p-SELEX (Plate)2'-Fluoro pyrimidines (2'-F-RNA) and AlexaFluor 488 LabeledNone of the other aptamers neutralized the cytotoxic effects of Stx2.N/AN/AN/AN/A
ABdb_0559 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-CA 20CACACACGGAACCCCGACAACATACATACGGTGAGGGTGG40N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 55% increase in the gated fluorescence intensities above background.8Flow Cytometry7 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0560 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-CA 20PTTCACGGTAGCACGCATAGG-CACACACGGAACCCCGACAACATACATACGGTGAGGGTGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 61% increase in the gated fluorescence intensities above background.8Flow Cytometry12 ± 1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0561 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 9GGGGAGGAGAAAAAGAGGACCAGAGTAACGATTCGTGGGG40N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.Showed a 55% increase in the gated fluorescence intensities above background.8Flow Cytometry71 ± 23 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0562 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 9PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAAAAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.D-Cells 9P showed an increase in the gated fluorescence above background by 65%.8Flow Cytometry44 ± 8 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0563 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-Cells 1PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAAAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT79N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow Cytometry20 ± 3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0564 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesE-Cells 1GGGGAGGAGAAAAGAGGACCAGAGTAACGATTCGTGGGG39N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0565 266787952015An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenesD-Cells 20PTTCACGGTAGCACGCATAGG-GGGGAGGAGAAATAGAGGACCAGAGTAACGATTCGTGGGG-CATCTGACCTCTGTGCTGCT80N/AssDNAStreptococcus Pyogenes M typesM proteins (M11)Identify aptamers selective for S. pyogenes and some of its M-types.N/A8Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0676 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P (Parental)AGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.5′-α-Gal, 3′-FAM α20A24P significantly increased phagocytosis in the presence of either transgenic mouse IgG or hIVIG.N/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM Labeled, 5′-α-Gal or Biotin-TEG modifiedN/AN/AN/AN/AN/A
ABdb_0677 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A2AGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCT69N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0678 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A3AGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCC52N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.5′-α-Gal α20A24P.A3 significantly increased phagocytosis in the presence of either transgenic mouse IgG or human IVIG.N/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM Labeled and 5′-α-GalN/AN/AN/AN/AN/A
ABdb_0679 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A4AGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCA53N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0680 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A5AGGTCAGATGGGGGGAAGACAC22N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0681 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A6AAGGCCGGGGTGAAGTGTAGAGGCCTA27N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0682 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A8TGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGC43N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0683 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A24P.A9AGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTAT57N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0684 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer15A3PTTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGGCATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0685 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A1PTTCACGGTAGCACGCATAGGCAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCCCATCTGACCTCTGTGCTGCT80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0686 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A8PAGCAGCACAGAGGTCAGATGCCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCCCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0687 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A8CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC40N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0688 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A9PAGCAGCACAGAGGTCAGATGCACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGGCCTATGCGTGCTACCGTGAA79N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0689 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A9CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG39N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0690 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A12PTTCACGGTAGCACGCATAGGGCCCGACACTCGTCCACCCGATACCTCTCATGTGTCCCCATCTGACCTCTGTGCTGCT78N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0691 259403162015Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer20A14PAGCAGCACAGAGGTCAGATGGGCATGGGGAAGAGAAAGCGGGATAACTTCGTTACCGGGCCCTATGCGTGCTACCGTGAA80N/AssDNAGroup A Streptococcus (GAS) M serotypesM proteinEvaluate the ability of alphamer (aptamer conjugated to an α-Gal epitope at its 5′ end) to redirect pre-existing anti-Gal antibodies to the GAS surface and promote opsonophagocytic clearance in vitro.N/AN/AFlow CytometryN/ATherapeuticsN/A5' or 3'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0713 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp1TGAGCCCAAGCCCTGGTATG-TTCTTCCCTTTTATTAGTCCTGTATTCCTCTACTGTTGCC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.With sandwich Sp1 and Sp20, the aptamer assay achieved a concentration range for S. sonnei of 10(2) cfu/mL to 10(7) cfu/mL and a detection limit of 30 cfu/mL.12Flow Cytometry5.98 ± 0.835 nMDetectionWhole Cell-SELEX5'-FAM Labeled and BiotinylatedN/AN/AN/AN/AN/A
ABdb_0714 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp13TGAGCCCAAGCCCTGGTATG-TATCCGATTTTATCTGTCCAATATTCCCTGTGTCTACCTT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry97.38 ± 36.2 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0715 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp16TGAGCCCAAGCCCTGGTATG-TATCTTCACTTGACCTTTACCGCAATCGTCCATGTATCTG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry682.2 ± 125 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0716 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp19TGAGCCCAAGCCCTGGTATG-TTCTACCCAACTGCTTTAATAGTCACTGTCATAGTTCCAC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0717 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp20TGAGCCCAAGCCCTGGTATG-TCGTCGATTATTGTTTCAGTTCAGTTCCCCCGCGTTCCGA-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.With sandwich Sp1 and Sp20, the aptamer assay achieved a concentration range for S. sonnei of 10(2) cfu/mL to 10(7) cfu/mL and a detection limit of 30 cfu/mL.12Flow Cytometry14.32 ± 2.19 nMDetectionWhole Cell-SELEX5'-FAM Labeled and HEX (hexachlorofluorescein) LabeledN/AN/AN/AN/AN/A
ABdb_0718 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp22TGAGCCCAAGCCCTGGTATG-CCTACCTTAGTCCGTTTCGCTGTGTTTGTCCAATATTCCT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry4.98 ± 0.357 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0719 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp23TGAGCCCAAGCCCTGGTATG-CTTCACTTGACCCTTATCGCTTCTAACACAACCGCTTTTT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry5.735 ± 0.478 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0720 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp28TGAGCCCAAGCCCTGGTATG-TATTCCCCCTTATTTTAGCCATTTTGTTACATCTTCCGTC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry72.89 ± 22.16 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0721 https://doi.org/10.1039/C5AY00214A2015Selection, identification, and application of dual DNA aptamers against Shigella sonneiSp29TGAGCCCAAGCCCTGGTATG-ATATTCGTTCTTTTCGCCTTAACTTCTCGTGTTTCCTTAC-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAShigella Sonnei (ATCC 51334)Whole cellIdentify aptamers and develop a sensitive sandwich detection method using the dual aptamers Sp1 and Sp20 to detect S. sonnei.N/A12Flow Cytometry47.83 ± 9.7 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0780 281211692016Development of a DNA aptamer that binds specifically to group A Streptococcus serotype M312L18AGCCTGTTGTGAGCCTCCTAAC-TCCTCGAGGGGGGGGGGATGAAAAGGAAAACGCAACAA-CATGCTTATTCTTGTCTCC785'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAGroup A Streptococcus (GAS) serotype M3Whole cellIdentify aptamers against S. pyogenes serotype M3.74% efficiency toward the S. pyogenes serotype M3.12Flow Cytometry7.47 ± 1.72 pMDetectionWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0781 281211692016Development of a DNA aptamer that binds specifically to group A Streptococcus serotype M312L12AGCCTGTTGTGAGCCTCCTAAC-GAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA-CATGCTTATTCTTGTCTCC785'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAGroup A Streptococcus (GAS) serotype M3Whole cellIdentify aptamers against S. pyogenes serotype M3.N/A12Flow Cytometry8.25 ± 1.43 pMDetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0782 272348802016Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolatesAci49GCCTGTTGTGAGCCTCCTAAC-TACATGGTCAACCAAATTCTTGCAAATTCTGCATTCCTACTGT-CATGCTTATTCTTGTCTCC835'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAAcinetobacter Baumannii (ATCC 19606)Whole cellIdentify aptamers against and develop a sandwich enzyme-linked aptamer sorbent assay (ELASA) for the rapid detection of A. baumannii from clinical isolates.Display detection limit of 10(3) CFU/ml, and the sensitivity of the test toward the clinical isolates was 95.47%.12Flow Cytometry7.547 ± 1.353 pMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0783 272348802016Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolatesAci55GCCTGTTGTGAGCCTCCTAAC-GTTACATGGTCAACCAAATTCTTGCAAATTCTGCATTCCT-CATGCTTATTCTTGTCTCC805'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAAcinetobacter Baumannii (ATCC 19606)Whole cellIdentify aptamers against and develop a sandwich enzyme-linked aptamer sorbent assay (ELASA) for the rapid detection of A. baumannii from clinical isolates.N/A12Flow Cytometry10.70 ± 2:561 pMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0814 267685822016DNA aptamers for the detection of Haemophilus influenzae type b by cell SELEXClone 63GCCTGTTGTGAGCCTCCTAAC-AGGAGGCGAACGGGCAGGTTCTTGGGGAGACAAGAATAAG-CATGCTTATTCTTGTCTCC805'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAHaemophilus Influenzae type b (Hib) (ATCC 10211)Whole cellIdentify an aptamer against Hib for the detection of H. influenzae.Clone 63 could detect Hib in patients’ CSF samples at 60 CFU/mL.7Flow Cytometry28.46 pMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0815 267685822016DNA aptamers for the detection of Haemophilus influenzae type b by cell SELEXClone 2, 42, 45GCCTGTTGTGAGCCTCCTAAC-GCCAGGTGTTTTGGCGGTAGGGGTGAAACAACAGGGGG-CATGCTTATTCTTGTCTCC785'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAHaemophilus Influenzae type b (Hib) (ATCC 10211)Whole cellIdentify an aptamer against Hib for the detection of H. influenzae.N/A7Flow Cytometry47.10 pMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0830 295945922017Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assayApt22GCTGTGTGACTCCTGCAA-GCGGGAAGAGGGTAAGGGGAGGGAGGGTAACGCGGAGAAGGCAA-GCAGCTGTATCTTGTCTCC815'-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)Antigen 85 (Ag85A) (FbpA)Identify aptamers that bind to and develop a graphene oxide-based fluorometric assay to detect FbpA.Display LOD in PBS and serum of 1.5 nM and 2.1 nM, respectively, with an analytical linear range from 5 to 200 nM of FbpA.12Flow Cytometry62.95 nMBiosensorMagnetic Bead (MB)-based Protein SELEXATTO647N-labeledN/AN/ABest CandidateN/AN/A
ABdb_0831 295945922017Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assayApt8GCTGTGTGACTCCTGCAA-TCAGGAAAGAACTTAGGGGTGGGAGGAGGGTATAAATACGGAAT-GCAGCTGTATCTTGTCTCC815'-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3'ssDNAMycobacterium Tuberculosis (M.Tb.)Antigen 85 (Ag85A) (FbpA)Identify aptamers that bind to and develop a graphene oxide-based fluorometric assay to detect FbpA.N/A12Flow Cytometry202 nMBiosensorMagnetic Bead (MB)-based Protein SELEXATTO647N-labeledN/AN/AN/AN/AN/A
ABdb_0834 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN08ATGAGAGCGTCGGTGTGGTAACTTAATCCTCTAGTTTTAATTCTAGATACGGCGCATGCACTCTATGTAGGAGGGTGCGGAAGTA855'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry54.9 ± 7.8 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0835 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN27ATGAGAGCGTCGGTGTGGTAACTAGTCTGATTTCTATTTCCTTTAATTAGTCTGCACACATTGCATTGTAGGAGGGTGCGGAAGTA865'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry28.5 ± 4.9 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0836 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN27.SHTAATTAGTCTGCACACATTGCATTGTAGGAGGGTGCGGAAGTA435'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry26.9 ± 5.6 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0837 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN08.SHATGCACTCTATGTAGGAGGGTGCGGA265'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry52.9 ± 10.4 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0838 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN17.SHATCCTCCTATCTATCGGTAGTTGTAGGAGGGTGCGG365'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry29 ± 7.3 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0839 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaJN21.SHAGAGCGTCGGTGTGGTAACTGTTCAGGAGGATGACATTGTCGCCT455'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry18.1 ± 3.4 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0840 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaSt17Lp21ATCCTCCTAGGTAACTGTTCAGGATGTAGGAGGGT355'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry16.9 ± 3.9 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0841 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaSt21Lp17AAGCGTCGGTGTTCTATCGGTAGTTGACACCGACGCCT385'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry13.5 ± 2 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0842 289379982017Selection of DNA aptamers specific for live Pseudomonas aeruginosaSt08Lp17ATGCACTCTTCTATCGGTAGTTGAGGGTGCGG325'-ATGAGAGCGTCGGTGTGGTA-N45-TACTTCCGCACCCTCCTACA-3'ssDNAPseudomonas Aeruginosa 692 (PA692) (ATCC 14502)Whole live and biofilm derived PA692 P. aeruginosa cellsIdentify aptamers to P. aeruginosa grown as biofilms in microfluidic cells.All aptamers were selective for P. aeruginosa with minimal cross-reactivity.7Flow Cytometry12.5 ± 2.4 nMDetectionWhole Cell-SELEXN/AAptamers had no intrinsic bacteriostatic and/or bactericidal activity.N/AN/AN/AN/A
ABdb_0843 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-6CGTGATGATGTTGAGTTG-GGGTGATGGGTGCATGTGATGAAAGGGGTTCGTGCTATGCTGTTTTGTCTAATAATACTAGTCCTTGCCAAGGTTTATTC-CAGTAATGCCAACCAATCT1175'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.66.26% fluorescent cell intensity for E. coli O157 with less cross-reactivity.10Flow Cytometry107.6 ± 67.8 pMDetectionWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0844 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-5CGTGATGATGTTGAGTTG-GAGTTGCGTTGCTGAATGCATGGGCTGTACTAGGTTGGTGCTACTCTGTAATGTCTACTATTACTATTCCATCGCAACGTATACTG-CAGTAATGCCAACCAATCT1235'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.57.41% fluorescent cell intensity for E. coli O157 with less cross-reactivity.10Flow Cytometry120.4 ± 32.2 pMDetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0845 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-1CGTGATGATGTTGAGTTG-GCCGAGATTGAAGCTGGTGACCATTGGCCTTACTGGGATGCTCTTGTGATTACTACTACTAATCGTTGTGAACGTAAATGC-CAGTAATGCCAACCAATCT1185'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.N/A10Flow CytometryN/ADetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0846 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-2CGTGATGATGTTGAGTTG-GTGTTAATCCGTGTATGCCATGAGACCGGTAAGTCCTATTTTCGCTCCTGGAATGGTACAAGCTCATGTCAAGATATACTC-CAGTAATGCCAACCAATCT1185'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.N/A10Flow CytometryN/ADetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0847 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-3CGTGATGATGTTGAGTTG-GGGCAGATCCGTGCACATGATCTTAGCCGTACGTGTTCTGCGGTATAGTCTATTCATACTACCACTTCGCAATGTATATCG-CAGTAATGCCAACCAATCT1185'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.N/A10Flow CytometryN/ADetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0848 288185572017DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX methodAM-4CGTGATGATGTTGAGTTG-GTCGAGTACGGATCACTTGGAGATTGGCCATCGAGCTATTCTGTAATGTGTAATCCTACTACACCTTCGCATCGTTAATGC-CAGTAATGCCAACCAATCT1185'-CGTGATGATGTTGAGTTG-80N-CAGTAATGCCAACCAATCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers for the detection of E. coli O157 and selectively distinguish the pathogenic strain from other strains.N/A10Flow CytometryN/ADetectionWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_0849 289414532017Development of a DNA Aptamer for Screening Neisseria meningitidis Serogroup B by Cell SELEXK3GCCTGTTGTGAGCCTCCTAACGCCAGGCGTTTTGGCCGTAGGCGTGGGAGACAAGAATAAGCA635'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNANeisseria Meningitidis Serogroup B (ATCC 13090)Whole cellIdentity aptamers bind to and detect N. meningitidis in patients’ CSF samples.Detected 10(2) CFU of CSF isolated N. meningitidis.6Flow Cytometry28.3 ± 8.9 pMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0850 289414532017Development of a DNA Aptamer for Screening Neisseria meningitidis Serogroup B by Cell SELEXK4N/AN/A5'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNANeisseria Meningitidis Serogroup B (ATCC 13090)Whole cellIdentity aptamers bind to and detect N. meningitidis in patients’ CSF samples.N/A6Flow Cytometry39.1 ± 8.6 pMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0851 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-5AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.Display a LOD of 10(4) with a linear range from 10(4) to 10(7) cfu/mL.12Flow Cytometry10.69 ± 0.89 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0852 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-2AGCAGCACAGAGGTCAGATG-GCCTGGCCAGGTGCCCCGATATAGCGACGCCTTGCCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0853 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-4AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCCCGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0854 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-12AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0855 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-1AGCAGCACAGAGGTCAGATG-CGCCCTACACCACTCTCGGAGCGCTGTACGGCATCCCTGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0856 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-15AGCAGCACAGAGGTCAGATG-GCCCGCACCACACACAGATGTCCATGTGTGCGCTGCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0857 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-11AGCAGCACAGAGGTCAGATG-GCCTGGCCAGGTGCCCCGATATAGCGACGCCTTGCCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0858 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-23AGCAGCACAGAGGTCAGATG-GCCTGGCCAGGTGCCCCGATATAGCGACGCCTTGCCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0859 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-14AGCAGCACAGAGGTCAGATG-GCCTGGCCAGGTGCGCCGATATAGCCTGCCCTTGCCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0860 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-18AGCAGCACAGAGGTCAGATG-GCCTGGCCACGTGCGCGGATATAGCCTGCCCTTGGCCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0861 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-9AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCCCGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0862 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-25AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCCCGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0863 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-28AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCCCGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0864 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-7AGCAGCACAGAGGTCAGATG-GCCCCGGACGGCGGGAAGCCTCGTACCCCGGGTGAGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0865 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-6AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0866 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-17AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0867 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-21AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0868 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-26AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0869 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-27AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0870 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-30AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0871 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-29AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCCGGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0872 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-3AGCAGCACAGAGGTCAGATG-TGCGTGAGCGGTAGCCGGGTACGACCCACTGTGGTTGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0873 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-13AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0874 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-16AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0875 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-22AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0876 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-24AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0877 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-19AGCAGCACAGAGGTCAGATG-GTCACACGGCCCGTCTGCGGTGTGGGACGCCCGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0878 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-20AGCAGCACAGAGGTCAGATG-GTCACACCGGCCGTCTCCGGTCTGGCACGCCGGCTGTGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0879 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-8AGCAGCACAGAGGTCAGATG-GCCCGCGCCCCTTCTGCCGTTGGTCGGAACCCCTGTGCGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0880 284413402017Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidumCCFM641-10AGCAGCACAGAGGTCAGATG-GCCCGCGCGGGTTCTGCCGTTGGTGGGAACCCCTGTGCGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Bifidum (ATCC 29521)Membrane proteinIdentify aptamers that bind to and develop a sandwich-type colorimetric bioassay for the detection of B. bifidum.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0881 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB2pAGCAGCACAGAGGTCAGATG-CCGGGCAGCGGTCAATGCCGCACCTTCCATATGATCGGGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.The per cent gated fluorescence intensity above the background was 8.9% for BB2p.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0882 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10pAGCAGCACAGAGGTCAGATG-GGCCCCCTGCCTGCCAAAAAGGTGTTGCCAGGTTGGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.The per cent gated fluorescence intensity above the background was 11.2% for BB10p.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0883 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16pAGCAGCACAGAGGTCAGATG-CTCCCAGGCCGTTGGGGCGTTGCCTGCGTGCACCCGGGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.The per cent gated fluorescence intensity above the background was 7.0% for BB16p.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0884 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB2CCGGGCAGCGGTCAATGCCGCACCTTCCATATGATCGGGG405'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.BB2 showed a lower binding affinity than the aptamer BB2p.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0885 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10GGCCCCCTGCCTGCCAAAAAGGTGTTGCCAGGTTGGCGGC405'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.Demonstrated higher binding affinity than the original full-length aptamer.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0886 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16CTCCCAGGCCGTTGGGGCGTTGCCTGCGTGCACCCGGGGC405'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.Demonstrated higher binding affinity than the original full-length aptamer.12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0887 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-6fGGCCCCCTGCCTGCCAAAAAGGTGTTGCCAGGTT345'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0888 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-10fGGCCCCCTGCCTGCCAAAAAGGTGTTGCCA305'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0889 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-14fGGCCCCCTGCCTGCCAAAAAGGTGTT265'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0890 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-2rCCCCCTGCCTGCCAAAAAGGTGTTGCCAGGTTGGCGGC385'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0891 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-9rCCTGCCAAAAAGGTGTTGCCAGGTTGGCGGC315'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0892 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB10-13rCCAAAAAGGTGTTGCCAGGTTGGCGGC275'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0893 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16-11fCTCCCAGGCCGTTGGGGCGTTGCCTGCGT295'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.LOD of the assay was 1000 cfu/mL with a linear range from 10(3) to 10(7) cfu/mL.12Flow Cytometry18.66 ± 1.41 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0894 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16-13fCTCCCAGGCCGTTGGGGCGTTGCCTGC275'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0895 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16-15fCTCCCAGGCCGTTGGGGCGTTGCCT255'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0896 https://doi.org/10.1039/C6RA27672E2017Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breveBB16-8rCCGTTGGGGCGTTGCCTGCGTGCACCCGGGGC325'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABifidobacterium Breve (ATCC 15700)Whole cellIdentify aptamers that bind to and develop colorimetric sandwich-type assay to detect B. breve in milk samples.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1012 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB10TAGCTCACTCATTAGGCAC-TCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.A dual-labelled sandwich detection system was developed using biotinylated RAB10 and FITC-labelled RAB35, with a limit of detection (LOD) of 10^2 CFU/mL of S. aureus, and showed 2.7-fold more signal than other bacteria.10Flow Cytometry46 ± 24 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1013 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB20TAGCTCACTCATTAGGCAC-GCGTTACGTTAGTGGCCGCCTATGAGGACAGGCGGTTGTA-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry128 ± 45 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1014 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB28TAGCTCACTCATTAGGCAC-TGGACGTCGTGGCGGAGGTTTTATAAAACGGCGCCACTGT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry49 ± 39 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1015 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB35TAGCTCACTCATTAGGCAC-GGGGGGTTGTGCCATTTAAGATGACCGGTTGCCGCGATTT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.A dual-labelled sandwich detection system was developed using biotinylated RAB10 and FITC-labelled RAB35, with a limit of detection (LOD) of 10^2 CFU/mL of S. aureus, and showed 2.7-fold more signal than other bacteria.10Flow Cytometry34 ± 5 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1016 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB1TAGCTCACTCATTAGGCAC-CGGGTGGGCTCCAATATGAATCGCTTGCCCTGACGCTATCT-GCATAGTTAAGCCAGCC775'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry56 ± 87 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1017 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB3TAGCTCACTCATTAGGCAC-CGTAGTCTAGTGTCGATTAGTTTCCTTGAGACCTTGTGCT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry37 ± 112 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1018 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB5TAGCTCACTCATTAGGCAC-CGTAGTCTAGTGTCGATTAGTTTCCTTGCTATTGCAGACCTTGTGCT-GCATAGTTAAGCCAGCC835'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow Cytometry58 ± 14 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1019 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB9TAGCTCACTCATTAGGCAC-TCGAGAGGGATCTCGGGGCGTGCGATGATTTTGCCTTCAT-GCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1020 291320302018Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface componentsRAB21TAGCTCACTCATTAGGCAC-GGGGGGTTGTGCCATTTAAGATGACCGGTTGCCAAGATG-GCATAGTTAAGCCAGCC755'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAStaphylococcus aureus (S. aureus) (RAB 9001)Whole cellIdentify aptamers that bind to and develop a dual-labelled sandwich detection system for the detection of S.aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1021 290985172018Influence of aptamer-targeted antibiofilm agents for treatment of Pseudomonas aeruginosa biofilmsPA-ap1 (F23)CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG60N/AssDNAPseudomonas Aeruginosa (ATCC 27853)Whole cellAptamer-ciprofloxacin-SWNTs complex for antibiofilm activity.90% inhibitory efficiency of complex aptamer-ciprofloxacin-SWNTs on biofilm formation.16Flow Cytometry17.27 ± 5.00 nMTargeted Delivery/TherapeuticsWhole Cell-SELEX5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_1029 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-12AGCAGCACAGAGGTCAGATG-GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.Limit of detection of 70 cfu/mL with a range of 70–7100 cfu/mL.12Flow Cytometry44 ± 5 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1030 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-2AGCAGCACAGAGGTCAGATG-GTTCGGGGTCGGGGTGAGTGGGGCCTAGGAGTGGGGGCGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1031 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-8AGCAGCACAGAGGTCAGATG-ATGGGGGGCGGGGAGGTGGGTACAGGGTCGGGGATGGCAG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1032 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-10AGCAGCACAGAGGTCAGATG-CGGGCGGGGCGTGGGGTGTTGGAGTGGAGGGCGGGGCGGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow Cytometry54  ±  8 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1033 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-15AGCAGCACAGAGGTCAGATG-CAGGGTGCGGGAGGGCCAAAGGGGGGAGGGCCCGGGGGGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1034 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-21AGCAGCACAGAGGTCAGATG-GGGGGAGGCGCCGGGCAGGGGGCTCGGGGGAGGCGGGCGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1035 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-26AGCAGCACAGAGGTCAGATG-TTCAGGGCGGGGTAAACGGGAGGTGGGGGGGGCTTGGGAC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow Cytometry130  ±  24 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1036 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-28AGCAGCACAGAGGTCAGATG-TAATACTACCAACTTCTTTGCCTGGCGTAAGTAACAGTCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow Cytometry132 ± 18 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1037 296986722018Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chickenS-32AGCAGCACAGAGGTCAGATG-TACACAATGCTTCGATAATTGACGCTACTTCATTTTATTA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNAStreptococcus Pyogenes (ATCC 49399)Whole cellIdentify aptamers that bind to and develop an aptamer-based fluorescent bioassay to detect S. pyogenes in the cooked chicken.N/A12Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1048 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-2TCAGCACGT-ATAAGCATGAATTGACCAACCTAAACTTATTCATTTTCCAGCACCTCTAATATTACTGGC-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.75% binding affinity to V. parahaemolyticus than to other foodborne bacteria (less than 18%).8Flow Cytometry10.3 ± 4.5 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1049 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-1TCAGCACGT-AGCCCTATTTGTTTTCTCTTGTCTCTGTACTGCTGATGTTGGTCATTCTCTTTTCTCGGT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry19.2 ±  2.8  nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1050 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-3TCAGCACGT-TATCTAGTCAGATATCCAGACAGTCGCGGCTGGAAGCTTCTGTTAAGAATTTGAGATACT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry25.2  ±  3.1 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1051 306850342018Selection of highly specific aptamers to Vibrio parahaemolyticus using cell-SELEX powered by functionalized graphene oxide and rolling circle amplificationApt-4TCAGCACGT-GCGGGCATTATTGCACTCCACGGCGAGTAGTGCTCACAGAGTCATTTACACCGGTCGTAT-TCCACTGAGAGATCC845'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cell (lipopolysaccharides (LPS) and Outer membrane protein (OMP) on V. parahaemolyticus cell surface)Identify aptamers against a V. parahaemolyticus.N/A8Flow Cytometry17.2  ±  5.1 nMDetectionAdvanced Cell-SELEX (with PC-GO and CRM-RCA)5ʹ-Phosphorylation or 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1052 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H7Apt-1TGAGCCCAAGCCCTGGTATG-TTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry18.97 ± 1.73 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1053 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H8Apt-2TGAGCCCAAGCCCTGGTATG-TGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry20.73 ± 2.29 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1054 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H9Apt-3TGAGCCCAAGCCCTGGTATG-TAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry16.44 ± 2.16 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1055 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H10Apt-4TGAGCCCAAGCCCTGGTATG-AGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry15.13 ± 0.88 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1056 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H11Apt-5TGAGCCCAAGCCCTGGTATG-CGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTT-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Lipopolysaccharide (LPS)Identify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.Apt-5 shows a strong affinity for all stages of bacterial growth (adjustment, log, and stationary phases).14Flow Cytometry9.04 ± 2.08 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1057 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H12Apt-6TGAGCCCAAGCCCTGGTATG-GTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry36.67 ± 4.55 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1058 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H13Apt-7TGAGCCCAAGCCCTGGTATG-TGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry17.11 ± 0.31 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1059 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H14Apt-8TGAGCCCAAGCCCTGGTATG-TCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCA-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry24.68 ± 1.95 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1060 297567742018Selection, Identification, and Binding Mechanism Studies of an ssDNA Aptamer Targeted to Different Stages of E. coli O157:H15Apt-9TGAGCCCAAGCCCTGGTATG-TTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAG-GGCAGGTCTACTTTGGGATC805'-TGAGCCCAAGCCCTGGTATG-N40-GGCAGGTCTACTTTGGGATC-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellIdentify aptamers that bind to different phases (adjustment phase, log phase, and stationary phase) of bacteria E. coli O157:H7 in the actual sample.N/A14Flow Cytometry20.99 ± 0.37 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1226 314037642019Rapid Detection of Mycoplasma-Infected Cells by an ssDNA Aptamer ProbeAptamer A15-1GTGGGGTTGAAAACGCCGGAGAGGGTGTGTGGGTGGGGTA405'-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3'ssDNAMycoplasma-Infected Cells (Jeko-1 lymphoma cells)Whole cellDevelop an aptamer probe for the rapid detection of mycoplasma-infected cells.Mycoplasma-positive cells showed a strong signal, but no signal was detected in the mycoplasma-negative cells.15Flow Cytometry24.5 nMDetectionWhole Cell-SELEX5'-Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1258 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA3PATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT87N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.Exhibits a wide linear detection range (from 10(2) to 10(7) cfu/mL), and the detection limit could be as low as 10 cfu/mL.N/AFlow Cytometry50.76 ± 8.92 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1259 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA1TCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACT40N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry32.53 ± 6.86 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1260 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA2GCAAAGAAACAGTGACTCGTTGA23N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry37.44 ± 6.98 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1261 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA3GCAACGAAACAGTGACTCGTTGA23N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry34.61 ± 3.47 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1262 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4CAACGAAACAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry28.65 ± 3.05 nMBiosensorWhole Cell-SELEX5'-FAM Labeled and 5'-Biotinylated (Biotin-TTTTTTTTT)N/AN/ABest CandidateN/AN/A
ABdb_1263 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA5AACGAAACAGTGACTCGTT19N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry422.0 ± 21.93 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1264 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA6ACGAAACAGTGACTCGT17N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry412.9 ± 27.12 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1265 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-1CAACGAAACATTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1004 ± 187.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1266 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-2CAACGAAACAGTTACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1103 ± 180.2 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1267 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-3CAACGAAACTGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry958.9 ± 183.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1268 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-4CAACGAAACAGCGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1070 ± 73.05 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1269 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-5CAACGAAACAGTGTCTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1119 ± 187.1 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1270 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-6CAACGAAATAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry1070 ± 125.4 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1271 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-7CAACGAACCAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry423.2 ± 36.91 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1272 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-8CAACGATACAGTGACTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry467.0 ± 33.38 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1273 307210472019Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus DeterminationA4-9CAACGAAACAGTGAGTCGTTG21N/AssDNAVibrio Parahaemolyticus (ATCC 17802)Whole cellIdentify an aptamer against and develop a colorimetric aptasensor based on magnetic nanoparticles (MNPs) and G4 DNAzyme to detect V. parahemolyticus in contaminated salmon samples.N/AN/AFlow Cytometry376.4 ± 54.25 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1284 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA1GACGCTTACTCAGGTGTGACTCG-TCCATACCTCCTATTCCTATCTGATCACCATGAGCTCTCGCCTGAACTGG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1285 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA2GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.VA2 could recognize different V. alginolyticus prevalent in Beibu Gulf with high specificity and affinity.9Flow Cytometry14.31 ± 4.26 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledNo cytotoxic effects shown in vitro and in vivo.N/ABest CandidateN/AN/A
ABdb_1286 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA3GACGCTTACTCAGGTGTGACTCG-TATCTTGTCAGCATATAGCCGCCCTGTCTCGTGCCTCTAATTGGGCTTGT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1287 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA4GACGCTTACTCAGGTGTGACTCG-CTTGGTGGGGGGTGGCGGGTTGGTGATTAGTTGCGTGTACAACCGTTGCG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1288 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA5GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1289 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA6GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1290 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA7GACGCTTACTCAGGTGTGACTCG-GGGGCACTCTGGGGAAGGACGTAAGCGATAGCCGACCTCGTGGAATATTA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1291 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA8GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.VA8 could recognize different V. alginolyticus prevalent in Beibu Gulf with high specificity and affinity but less potent than VA2.9Flow Cytometry90.00 ± 13.51 nMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FAM LabeledNo cytotoxic effects shown in vitro and in vivo.N/ABest CandidateN/AN/A
ABdb_1292 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA9GACGCTTACTCAGGTGTGACTCG-TCACCGTCATACACGGTCACCTGTTCTGCTATCACCCTGACGTGATTGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1293 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA10GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1294 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA11GACGCTTACTCAGGTGTGACTCG-TGGCGCAAGGGAAGGTGTTGGGGGATGGTTGGTGGGGTGTGTGGTTGTAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1295 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA12GACGCTTACTCAGGTGTGACTCG-ATGGCTGACTATATAAGAGAACGGGGGCTGCGGTGGTCCCGATCTGGCAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1296 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA13GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1297 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA14GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1298 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA15GACGCTTACTCAGGTGTGACTCG-TGGCGATGTACGCACCGCCCTCAGACTCATTCAGCATATAGCCTAGTAAC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1299 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA16GACGCTTACTCAGGTGTGACTCG-GGGGTGGATTGGGTGGGGTGGTTGGTTCGTTTTAAAGTACTGTGTAAGCG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1300 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA17GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1301 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA18GACGCTTACTCAGGTGTGACTCG-GCTGCGTTAACATATAGGGCATTTGAGTGCGCGCATAGGGTTGTGGCGAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1302 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA19GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1303 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA20GACGCTTACTCAGGTGTGACTCG-TCCAGTCAGTATATAACTCCTCGACCCTGTGATCGGCTACGGTGGCAGTA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1304 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA21GACGCTTACTCAGGTGTGACTCG-GAGTGCTCGGTTGGTGTTGGGGGAGGGTGGTGGGTGACCATGCTACATGT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1305 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA22GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1306 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA23GACGCTTACTCAGGTGTGACTCG-GGGCGGGTGGACGGGGGTGGTATGGAGGTTGGAGTAAGCGGTTTCACCGA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1307 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA24GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1308 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA25GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1309 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA26GACGCTTACTCAGGTGTGACTCG-TCTGGTAGCATATAGCCGGCAGAGCACGGTCCCCTCATGGTTGGCACGTA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1310 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA27GACGCTTACTCAGGTGTGACTCG-GCCATGCTCACTATATAAGATATGTAAGTTGGACTACATATGTATGGCTG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1311 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA28GACGCTTACTCAGGTGTGACTCG-GCTGTGATCGTATGGACGCTGCAGTTGGATGACGCTATGAGGTATTCCAA-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1312 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA29GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1313 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA30GACGCTTACTCAGGTGTGACTCG-CGTTTTATTGGTGTGGGGCTGGGGTGGTGGGTGGCTCTACTGGTTCCGTT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1314 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA31GACGCTTACTCAGGTGTGACTCG-TCGGTCGGGTGGTTGGGGCGGGTGGTCGGTTTTTAAGTTGTGTCATTGTC-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1315 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA32GACGCTTACTCAGGTGTGACTCG-CACTATTGTCAAGTCACGAGTTAGGTACCTATGTGATCTGAGGTATCAA-CGAAGGACGCAGATGAAGTCTC945'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1316 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA33GACGCTTACTCAGGTGTGACTCG-CTACTCAGGTGACCCTCAAACGTATCTAGTTTGGCAGCGAGGTTCCTTCG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1317 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA34GACGCTTACTCAGGTGTGACTCG-TTGCGAATATAGCGACGAGTATCGTCAGAGTCGCCGGGTCCAGGTTCGA-CGAAGGACGCAGATGAAGTCTC945'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1318 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA35GACGCTTACTCAGGTGTGACTCG-GGTGGTGGGGATTTGGGGTGGTTGGTTCATCTATCTGGTCGTTACCGGGG-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1319 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA36GACGCTTACTCAGGTGTGACTCG-CGCGGCACCAGCACGACGGTTCGATTGGCTGCGGAAACCACGCACTGCAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1320 308595982019Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticusVA37GACGCTTACTCAGGTGTGACTCG-GGTGTTGGTGGGTGGCGGGGTGGTGGTTGGTGTTCCCGAACCTGATGAAT-CGAAGGACGCAGATGAAGTCTC955'-GACGCTTACTCAGGTGTGACTCG-50N-CGAAGGACGCAGATGAAGTCTC-3'ssDNAVibrio AlginolyticusWhole cellIdentify aptamers targeted against viable V. alginolyticus.N/A9Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1321 315633152019Rapid identification and quantitation of the viable cells of Lactobacillus casei in fermented dairy products using an aptamer-based strategy powered by a novel cell-SELEX protocolApt-1ATCGTATACCTAGAAGATATGACCGGCAGTTGATGAGATAGAGGCGCTGGTGGGTAGATA605'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNALactobacillus Casei (ATCC 393)Whole cellAn aptamer-based strategy using PEG and chitosan-modified graphene oxide, with complementary ring-mediated RCA, was developed for qualitative and quantitative detection of viable L. casei in dairy products.N/A8Flow Cytometry21.1 ± 2.7 nMDetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1322 315633152019Rapid identification and quantitation of the viable cells of Lactobacillus casei in fermented dairy products using an aptamer-based strategy powered by a novel cell-SELEX protocolApt-2GGCTCACCTACAGGCTGCGGAGTCATAGCATCGTGACAGAGTCGAGTGTCGACTATACGT605'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNALactobacillus Casei (ATCC 393)Whole cellAn aptamer-based strategy using PEG and chitosan-modified graphene oxide, with complementary ring-mediated RCA, was developed for qualitative and quantitative detection of viable L. casei in dairy products.Achieved a detection limit of 10(5) cfu/mL and selective detection of L. casei in commercial dairy drinks, with a dynamic range of 10(5) to 10(9) cfu/mL.8Flow Cytometry19.3 ± 3.7 nMDetectionWhole Cell-SELEX3'-Biotinylated (biotin-AAAAAAAAAA)N/AN/AN/AN/AN/A
ABdb_1323 315633152019Rapid identification and quantitation of the viable cells of Lactobacillus casei in fermented dairy products using an aptamer-based strategy powered by a novel cell-SELEX protocolApt-3TCGAAGTGAGCGCGCGGTGTGGTGACTGTGTTGCAGATGGATGCATGGAGGTGATGATGA605'-TCAGCACGT-N60-TCCACTGAGAGATCC-3'ssDNALactobacillus Casei (ATCC 393)Whole cellAn aptamer-based strategy using PEG and chitosan-modified graphene oxide, with complementary ring-mediated RCA, was developed for qualitative and quantitative detection of viable L. casei in dairy products.Achieved a detection limit of 10(5) cfu/mL and selective detection of L. casei in commercial dairy drinks, with a dynamic range of 10(5) to 10(9) cfu/mL.8Flow Cytometry15.6 ± 4.1 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1367 324995572020Identification of two aptamers binding to Legionella pneumophila with high affinity and specificityR10C5GCAATGGTACGGTACTTCCGGACAGTGCTGAAAACTGTGACCCCCCAAAAGTGCACGCTACTTTGCTAA695'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNALegionella Pneumophila (Lp 120292)Whole cellIdentify an aptamer against Lp and utilise it as a biorecognition element in a biosensor to detect Lp in real-time and in situ.Around 60% of lp120292 cells are stained by R10C5 and 20% of Pseudomonas strains.10Flow Cytometry116 nMBiosensorWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/APatent application no US 16/850,355
ABdb_1368 324995572020Identification of two aptamers binding to Legionella pneumophila with high affinity and specificityR10C1GCAATGGTACGGTACTTCCCCACCCCACGCTGCTCCCAAAAGTGCACGCTACTTTGCTAA605'-GCAATGGTACGGTACTTCC-N45-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNALegionella Pneumophila (Lp 120292)Whole cellIdentify an aptamer against Lp and utilise it as a biorecognition element in a biosensor to detect Lp in real-time and in situ.R10C1 shows significantly more binding to Lp than to Pseudomonas.10Flow Cytometry135 nMBiosensorWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/APatent application no US 16/850,355
ABdb_1415 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV8AGTATACGTATTACCTGCAGC-CAATCATGACCGCCCACCTCACTCG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cell (Cell wall protein)Isolate aptamers that specifically bind V. vulnificus across all culture phases.The LOD of the V8 from cytometry is 29.96 CFU/mL, and the linear range is 102–5 × 105 CFU/mL.13Flow Cytometry11.22 ± 1.30 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AV8 and V13 can tolerate diluted serum as well as oyster infusion.Best CandidateN/AN/A
ABdb_1416 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV9AGTATACGTATTACCTGCAGC-CCTGGACATCATTGAGTACTCGTCT-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1417 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV11AGTATACGTATTACCTGCAGC-TCCCAACCAATACCAGTACGTTGTA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1418 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV12AGTATACGTATTACCTGCAGC-TATGGATTTGCGTCATGTTTATGTG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1419 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV13AGTATACGTATTACCTGCAGC-CCAACCCTATGCTTCAACGGTCTTT-GCAAAGATCTCCGAGATATCG675'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow Cytometry15.47 ± 0.39 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AV8 and V13 can tolerate diluted serum as well as oyster infusion.Best CandidateN/AN/A
ABdb_1420 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV18AGTATACGTATTACCTGCAGC-TGTGGGTGGGTGGGTGGTATCTGCA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1421 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV20AGTATACGTATTACCTGCAGC-CATCCCCTCTCCTGTTGCCCTGACA-GCAAAGATCTCCGAGATATCG675'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1422 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV28AGTATACGTATTACCTGCAGC-CCTGGACATCATTGAGTACTCGTCT-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1423 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV31AGTATACGTATTACCTGCAGC-TGTGGGTGGGATTAGGTTCGGGTGG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1424 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV38AGTATACGTATTACCTGCAGC-CCAGACTTCAATCGCGTCAACCGTT-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1425 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV39AGTATACGTATTACCTGCAGC-TGATGGTTGTATGACTGGATGTCAA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1426 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV40AGTATACGTATTACCTGCAGC-TCCCCTTTGCATGGCGGTGACACTG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1427 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV41AGTATACGTATTACCTGCAGC-CACCTAGAACACATTGCAACATTAG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1428 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV44AGTATACGTATTACCTGCAGC-TGCTCCTCGACTGTTGTTAATCGTG-GCAGATCTCCGAGATATCG655'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1429 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV49AGTATACGTATTACCTGCAGC-TGACATCGTCTGACCTCCACAAGCA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1430 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV53AGTATACGTATTACCTGCAGC-TGGGTCCGTATGTTGGTGTATGTGA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1431 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV59AGTATACGTATTACCTGCAGC-TGTATACCCGACCGTACCGACGTAA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1432 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV69AGTATACGTATTACCTGCAGC-TCACCTTCACACACTCCCTTCTTCG-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1433 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyV71AGTATACGTATTACCTGCAGC-CCTGTACAAGCAGTATGTCAGCTGA-GCAAGATCTCCGAGATATCG665'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.N/A13Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1434 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyTV8CAATCATGACCGCCCACCTCACTCG255'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.The fluorescent intensity of V. vulnificus was significantly greater than that of other species.13Flow Cytometry17.44 ± 1.30 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1435 354936822020Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technologyTV13CCAACCCTATGCTTCAACGGTCTTT255'-AGTATACGTATTACCTGCAGC-N25-GCAAGATCTCCGAGATATCG-3'ssDNAVibrio Vulnificus (ATCC 27562)Whole cellIsolate aptamers that specifically bind V. vulnificus across all culture phases.The fluorescent intensity of V. vulnificus was significantly greater than that of other species.13Flow Cytometry13.21 ± 2.19 nmBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1441 324711282020Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell CultureA15-1GTGGGGTTGAAAACGCCGGAGAGGGTGTGTGGGTGGGGTA40N/AssDNAMycoplasma HyorhinisMycoplasma hyorhinis-infected cellsDevelop an aptamer cocktail for detecting multiple species of mycoplasma in infected cell lines.A15-1 could bind to M. hyorhinis, and mixed mycoplasma-infected cells were detectable within 30 min.N/AFlow CytometryN/ADetectionN/A5'-Biotinylated and Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1442 324711282020Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell CultureA16-1YTGGGTGGGGTTGTCGCTAGGGGTTTAAGGGGTCGTCGTGA40N/AssDNAMycoplasma HyorhinisMycoplasma hyorhinis-infected cellsDevelop an aptamer cocktail for detecting multiple species of mycoplasma in infected cell lines.A16-1Y could bind to M. hyorhinis and mixed mycoplasma-infected cells.N/AFlow CytometryN/ADetectionN/A5'-Biotinylated and Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1443 324711282020Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture#1JATCCAGAGTGACGCAGCAGCCAACGTGCTTTCTACCTTATTTTCCGTCACTCTCACTCTGGACACGGTGGCTTAGT76N/AssDNAMycoplasma HyorhinisMycoplasma hyorhinis-infected cellsDevelop an aptamer cocktail for detecting multiple species of mycoplasma in infected cell lines.#1J could bind to unclassified mycoplasma-infected cells, but not M. hyorhinis-infected cells.N/AFlow CytometryN/ADetectionN/A5'-Biotinylated and Cyanine3 (Cy3) LabeledN/AN/AN/AN/AN/A
ABdb_1476 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp1N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry18.10 ± 6.2 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1477 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp2N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry13.38 ± 3.8 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1478 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp3N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry11.46 ± 4.1 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1479 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp4N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry14.18 ± 4.3 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1480 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp5N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry21.78 ± 9.2 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1481 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp6TAGGGAAGAGAAGGACATATGAT-GCGCTGTGCGGATGTCATGATGTGCCTCTTCCCTGTGTCCGC-TTGACTAGTACATGACCACTTGA885'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.The detection limit of this aptasensor was as low as 10(5) CFU/ml within 10 minutes and a linear range of 10(5)-10(8) CFU/ml.10Flow Cytometry9.82 ± 3.6 nMBiosensorWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/ASubmission Number Petty patent no.: 1803001734
ABdb_1482 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp7N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry12.85 ± 4.1 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1483 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp8N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry32.90 ± 17.4 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1484 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp9N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry12.18 ± 4.3 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1485 319482822020Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samplesAp10N/AN/A5'-TAGGGAAGAGAAGGACATATGAT-N40-TTGACTAGTACATGACCACTTGA-3'ssDNAListeria Monocytogenes (ATCC 13932), Salmonella spp. (S. typhimurium ATCC 14028, S. anatum DMST 23906, S. albony DMST 24240, S. enteritidis DMST 15676) and Escherichia Coli (E. Coli) (ATCC 25922)Whole cellIdentify aptamers against Salmonella spp., Listeria monocytogenes, and Escherichia coli and using gold nanoparticles, develop a colorimetric aptasensor for simultaneous detection.N/A10Flow Cytometry32.85 ± 12.2 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/ASubmission Number Petty patent no.: 1803001734
ABdb_1490 355161862020Selection of potential aptamers for specific growth stage detection of Yersinia enterocoliticaM1AGCAGCACAGAGGTCAGATGATATAACCTTAATAAATAAAATATAAATTATTTAATCTTACCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-40N-TTCACGGTAGCACGCATAGG-3'ssDNAYersinia Enterocolitica (CICC 21669)Whole cellIdentified aptamers bound to the different growth stages of Y. enterocolitica.Showed good affinity to all different stages of bacteria (adjustment phase, log phase, and pre-stationary phase).10Flow Cytometry37.93 ± 7.88 nMDetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1491 355161862020Selection of potential aptamers for specific growth stage detection of Yersinia enterocoliticaM5AGCAGCACAGAGGTCAGATGCTTGGTTCCACCGTACTGACTGTAGTAAAATCTGATCACTCCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-40N-TTCACGGTAGCACGCATAGG-3'ssDNAYersinia Enterocolitica (CICC 21669)Whole cellIdentified aptamers bound to the different growth stages of Y. enterocolitica.Showed good affinity to all different stages of bacteria (adjustment phase, log phase, and pre-stationary phase).10Flow Cytometry74.96 ± 21.34 nMDetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1492 355161862020Selection of potential aptamers for specific growth stage detection of Yersinia enterocoliticaM7AGCAGCACAGAGGTCAGATGTAGTCGGTCTTCTTGTTTGAAACTGCTAATTTTGAAAAAACCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-40N-TTCACGGTAGCACGCATAGG-3'ssDNAYersinia Enterocolitica (CICC 21669)Whole cellIdentified aptamers bound to the different growth stages of Y. enterocolitica.Showed good affinity to all different stages of bacteria (adjustment phase, log phase, and pre-stationary phase).10Flow Cytometry73.02 ± 18.76 nMDetectionWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1494 329948962020Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1V.ch27GCCTGTTGTGAGCCTCCTAAC-GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCAC-CATGCTTATTCTTGTCTCC815'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAVibrio Cholerae O1 [Inaba (ATCC 39315) and Ogawa]Whole cellIdentify aptamers and develop aptamer-nanobody-based ELISA for V. cholerae O1 detection.The binding efficiency for V.ch27 was 53.3%.12Flow Cytometry20.186 ± 3.655 pMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1495 329948962020Aptamer-nanobody based ELASA for detection of Vibrio cholerae O2V.ch47GCCTGTTGTGAGCCTCCTAAC-CGTATTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTC-CATGCTTATTCTTGTCTCC795'-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3'ssDNAVibrio Cholerae O1 [Inaba (ATCC 39315) and Ogawa]Whole cellIdentify aptamers and develop aptamer-nanobody-based ELISA for V. cholerae O1 detection.Identify 10(4) CFU/ml with 25 pM of biotinylated aptamer and only 20 μg/ml of VHH without any cross-reactivity.12Flow Cytometry15.404 ± 4.776 pMDetectionWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1570 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI05TAGCTCACTCATTAGGCACGACTCACTCTTGAAGGGGTGAGGCATGGTGGTATCAGCATAGTTAAGCCAGCC725'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry144.4 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1571 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI08TAGCTCACTCATTAGGCACATGCACCGAGGTCAGAAGTGCCTGTAATACACACCCCTCGGCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry301.4 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1572 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI 9TAGCTCACTCATTAGGCACCGAGTGCAAGATGTCCTACCCGATGAAGTAGGTTGGGTCTGCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry279.7 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1573 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI23TAGCTCACTCATTAGGCACCTTGGCTTAAAATGATAGCTAGTGGCAGATTGTTAATTTGGCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.The assay is able to detect 10(2) CFU/mL cells.10Flow Cytometry231.2 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1574 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI27TAGCTCACTCATTAGGCACATGGCAAGGTTGCCTTTTTGAGCGCGCTGCATAGTTAAGCCAGCC645'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry328.9 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1575 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI31TAGCTCACTCATTAGGCACCAGCCGGAAGGACCAGCTAGCTCACTCATTAGGCACCGAAGTGTGGTGCATAGTTAAGCCAGCC835'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry186.1 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1576 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI37TAGCTCACTCATTAGGCACGAACGGGCTTGTCTTTCCATAATTCGTGCGAAAGTGCCGCATAGTTAAGCCAGCC745'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry176.7 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1577 335829482021Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water SamplesSHI42TAGCTCACTCATTAGGCACTAGCGGAAGCTTAGTGTAAACGAGTGATAATGTGTTATGTGCATAGTTAAGCCAGCC765'-TAGCTCACTCATTAGGCAC-40N-GCATAGTTAAGCCAGCC-3'ssDNAShigella Flexneri (ATCC 9199)Whole cellIdentify aptamers and develop a fluorescence-based assay for direct detection of S. flexneri from water samples.N/A10Flow Cytometry173.6 nMDetectionWhole Cell-SELEX5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1607 358467532022Selection and Identification of a DNA Aptamer for Multidrug-Resistant Acinetobacter baumannii Using an In-House Cell-SELEX MethodologyA01CAGGGGACGCACCAAGG-TTTTGTTTTTTCTTTGCTTCTTTTTGCTTTTTTTT-CCATGACCCGCGTGCTGCGTGA745'-CAGGGGACGCACCAAGG-N35-CCATGACCCGCGTGCTGCGTGAT-3'ssDNAMultidrug-resistant (MDR) Acinetobacter BaumanniiWhole cellIdentify aptamers against MDR A. baumannii.Preferential binding to A. baumannii over other species. 25% of positive events for A. baumannii above the 12.66% of the negative control.7Flow CytometryN/ADetectionWhole Cell-SELEX5'-FAM LabeledAptamer binding does not seem to interfere with the A. baumannii cell growth even after 24h (5.88E+02 x 1.45E+03; p=0.700).N/AN/AN/AN/A
ABdb_1673 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersEC1GGGGTCATAGTATCCTAGTTG-AATGATGACATTCCGAGGTACCCAGAGTGCGATACTAACCGGCCTGCTTG-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 25922)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry1.165 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1674 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersEC2GGGGTCATAGTATCCTAGTTG-CTCGCGCGCCCAATGTTTGCATCGATCTGATGGGCATAACGGCCTTGAAT-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 25922)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry10.26 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1675 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersEC3GGGGTCATAGTATCCTAGTTG-CCGACGAGACTTCCACAACGCTGCCAAGCCATCGTCCCCCCGCACTCAGG-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 (ATCC 25922)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry3.17 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1676 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersLM1GGGGTCATAGTATCCTAGTTG-CCGCCCGTAGAGGATTGTAGCCGCCTTTCGCCAACCTAAACCGAGACCTG-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry38.3 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1677 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersLM2GGGGTCATAGTATCCTAGTTG-TCGTTCATCTGAGCTCCTGGTATCGCGTGTGTATACGAGCCTCACATTCA-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry9.03 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1678 357009362022SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamersLM3GGGGTCATAGTATCCTAGTTG-CCGTCCCGGTAGTCTGAGCTTACAATGTCTGATTGGAAATGCACACCAAG-CCTGTAATTTATCTTCTGGAGCT945'-GGGGTCATAGTATCCTAGTTG-N50-CCTGTAATTTATCTTCTGGAGCT-3'ssDNAListeria Monocytogenes (ATCC 19115)Lipopolysaccharide (LPS)Identify aptamers that bind to LPS on Gram-negative bacteria and inhibit the LPS-induced inflammatory response, thereby inhibiting bacterial infections.When aptamers bind to the LPS, the macrophage polarization ability of LPS can decrease, and the secreted nitrite level diminishes.8Flow Cytometry8.89 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1725 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA37 (SA-10R-37)GCTTCCAGCTTATTGAAT-AAAGACGGGGGGGGGGACCGGCGTATGAGTGAAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.Achieved a limit of detection (LOD) of 39 CFUs and 414 CFUs in buffer and spiked tap water samples, respectively, with the cognate pair of SA37@Cy5 and SA81@FAM.10Flow Cytometry16.5 ± 3.41 nMBiosensorWhole Cell-SELEX5'-Cyanine5 (Cy5) LabeledN/AN/ABest CandidateN/AN/A
ABdb_1726 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA81 (SA-10R-81)GCTTCCAGCTATTGAA-AACGAGGCGCAGGGGGAGGGGGTGGTACAGATAAGATGGGG-GCGCTGAAGCGCGGAAGC755'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.Achieved a limit of detection (LOD) of 39 CFUs and 414 CFUs in buffer and spiked tap water samples, respectively, with the cognate pair of SA37@Cy5 and SA81@FAM.10Flow Cytometry14.47 ± 8.18 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1727 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA25 (SA-9R-25)GCTTCCAGCTTATTGAAT-GGGGAAGGTCGTCCGACGAACCCGGTCAGATAGGGTGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry44.92 ± 1.36 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1728 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA28 (SA-10R-28)GCTTCCAGCTTATTGAAT-GCGGCCACGGAGGGGGTGCCGGGCGTGGAATAAGATGTGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry77 ± 1.22 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1729 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA35 (SA-10R-35)GCTTCCAGCTTATTGAAT-CACAGGTGTGGGGAGGTCCCCATGGAGGTGGTTCAATG-GCGCTGAAGCGCGGAAGC745'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry58.77 ± 0.73 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1730 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA40 (SA-10R-40)GCTTCCAGCTTATTGAAT-CGACGTGAAGCAATCATGGGTGGGGTACGTCGGGTCATGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow Cytometry126.95 ± 0.51 nMBiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1731 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-9R-12GCTTCCAGCTTTATGAAT-TGGGCGTGGCGACGGTGTCAGGTCGGGGCGGTAAGACGAGG-GCGCTGAAGCGCGGAAGC775'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1732 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-9R-57GCTTCCAGCTTATTGAAT-GGAACGAGATGGGGCATGGCGCCACGGAGGGGAATCAAGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1733 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-1GCTTCCAGCTTATTGAAT-CGAGGGTAAGGGAAGTATGGCCGGTGCCCTGGAGTCAATG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1734 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-18GCTTCCAGCTTATTGAAT-CGGGTGGGGGGAGCGACAGACGGAAAAGCCTGGGTCAATAG-GCGCTGAAGCGCGGAAGC775'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1735 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-55GCTTCCAGCTTATTGAAT-GGAGGGGCAGGGCATGGAGCTCGACATTCATGAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1736 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-59GCTTCCAGCTTATGGAAT-GTGCCGAGGCGATACATGCACAGGCATGGTGGGATAAGGTCGGG-GCGCTGAAGCGCGGAAGC805'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1737 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-69GCTTCCAGCTTATTGAAT-CGGAGAGTCTGGCGCAGCCCTACGCCGATGTAAGATGGGG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1738 348473602022A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureusSA-10R-72GCTTCCAGCTTATTGAAT-CGAGGTGTGGGAGACAACGCTCCGTGACCGAGGGGAAATG-GCGCTGAAGCGCGGAAGC765'-GCTTCCAGCTTATTGAAT-40N-GCGCTGAAGCGCGGAAGC-3'ssDNAStaphylococcus aureus (S. aureus) (ATCC 25923)Whole cellA sandwich-type signal-on electrochemical aptasensor using a cognate aptamer pair was developed for the detection of S. aureus.N/A10Flow CytometryN/ABiosensorWhole Cell-SELEXN/AN/AN/AN/AN/AN/A
ABdb_1801 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB3AGCAGCACAGAGGTCAGATG-AGGCCCGGGTTTGGTTCTGGGGTTGGCGGGCTGCGTGAGC-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1802 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB4AGCAGCACAGAGGTCAGATG-GAAGGGTTTGGTGGTAAATTGCGTGGTTGGCTGTTGATGG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1803 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB6AGCAGCACAGAGGTCAGATG-GGCGGGTTTGGATCTTTGGTTGGCGCTTGTTTCTTTATGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1804 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB7AGCAGCACAGAGGTCAGATG-TCGGTCGGGTTTGGGTGTTGGTTGGCGATAAAGATAACTG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow Cytometry19.64 ± 4.11 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1805 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB11AGCAGCACAGAGGTCAGATG-GTGTAGGTCAATCGACGCCACCCATAGCAACCATCCTGCG-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow CytometryN/ABiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1806 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB14AGCAGCACAGAGGTCAGATG-GTGGGCAGTGGGGGGAGGGGGAGGTGGAGGTTAGACGTGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.N/A16Flow Cytometry28.64 ± 3.10 nMBiosensorWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1807 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB15AGCAGCACAGAGGTCAGATG-GGCGGGTTTGGATCTTTGGTTGGCGCCTGTTTCTTTATGA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.Achieve highly sensitive detection of B. cereus as low as 10 cfu/mL.16Flow Cytometry16.13 ± 4.98 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1808 https://doi.org/10.1016/j.aac.2023.07.0052023DNA aptamers selection and characterization for development of impedimetric aptasensor for Bacillus cereus at different growing stagesB16AGCAGCACAGAGGTCAGATG-ATATGTTTACGCCAGTGGTATTATTGGGGTTGATATGTCA-CCTATGCGTGCTACCGTGAA805'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'ssDNABacillus Cereus (CICC 10041)Whole cellIdentify aptamers against B. cereus across different growth phases and develop an impedance-based aptasensor immobilized on a GCE/Au surface for its detection.Achieve highly sensitive detection of B. cereus as low as 10 cfu/mL.16Flow Cytometry20.67 ± 5.23 nMBiosensorWhole Cell-SELEX5'-Thiolated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1810 374372662023Specific Instantaneous Detection of Klebsiella pneumoniae for UTI Diagnosis with a Plasmonic Gold Nanoparticle Conjugated AptasensorKPBA1GGCTGGATGGGGCGTGT-GGAGCCCCGTTAGAATATCAGAGGTGGTGG-CAACGGTGCGGACAGCG645'-GGCTGGATGGGGCGTGT-30N-CAACGGTGCGGACAGCG-3'ssDNAKlebsiella Pneumoniae (MTCC-7028)Whole cellDeveloped localized surface plasmon resonance (LSPR) aptasensor using a tailor-made plasmonic aptamer-gold nanoparticle (AuNP) for detection of Klebsiella pneumoniae.LoD as low as 3.4 × 10(3) CFU/mL within 5 min.10Flow CytometryN/ABiosensorWhole Cell-SELEX5′-Thiolated (5′-/5ThioMC6-D) and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1871 386698462024Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensorsS. enterica aptamerTATGGCGGCGTCACCCGACGGGGACTTGACATTATGACAG40N/AssDNASalmonella Typhimurium (S. Typhimurium) (ATCC 14028)Whole cellDeveloped an aptamer-functionalized CNT-FET biosensor for the detection of these foodborne pathogens.Could detect S. enterica at a limit of detection (LOD) as low as 1 CFU in PBS buffer with a linear range from 62.5–6.4 × 10(4) CFU/mL.N/AFlow Cytometry8.9 ± 2.0 nMBiosensorN/A5'-Thiolated (HS-(CH2)6), 5'-FAM Labeled and 3'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1872 386698462024Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensorsS. aureus aptamerAACGAGGCGCAGGGGGAGGGGGTGGTACAGATAAGATGGGG41N/AssDNAStaphylococcus aureus (S. aureus) (CMCC(B) 26003)Whole cellDeveloped an aptamer-functionalized CNT-FET biosensor for the detection of these foodborne pathogens.Could detect S. aureus at a limit of detection (LOD) as low as 1.2 CFU in PBS buffer with a linear range from 10(2)-1.28 × 10(4) CFU/mL.N/AFlow Cytometry0.65 ± 0.2 μMBiosensorN/A5'-Thiolated (HS-(CH2)6), 5'-FAM Labeled and 3'-Cyanine5 (Cy5) LabeledN/AN/AN/AN/AN/A
ABdb_1883 414312702025Bioimaging with fluorescent nucleic-acid aptamers for the specific detection and quantification of Pseudomonas aeruginosa alone and in heterogeneous bacterial populationsF23ATACCAGCTTATTCAATT-CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG-AGATAGTAAGTGCAATCT96N/AssDNAPseudomonas Aeruginosa (ATCC 15692)Whole cellDevelop a fluorescent aptamer for detecting Pseudomonas aeruginosa.73.79% of live P. aeruginosa cells (3102/4204) were labeled by the Cy5‐F23 aptamer, compared to only 0.06% of live S. aureus (4/6767).15Flow Cytometry17.27 ± 5.00 nMImagingWhole Cell-SELEX5'-Cyanine5 (Cy5) LabeledN/AThe aptamer remains highly stable for 72 hours.N/AN/AN/A
ABdb_1896 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS8CGTACGGTCGACGCTAGC-ACACCCCCCAGGATCATAAATTCTCTCGCTGTAGC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1897 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS10CGTACGGTCGACGCTAGC-ACCAGCCATAGTCACATCAAAACTAACTCACATTC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1898 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS12CGTACGGTCGACGCTAGC-CCCATACTCATCACCATTCACATCACTCACCTAGC-CACGTGGAGCTCGGATGC715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1899 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS1GGATCCGAGCTCCACGTG-GCTAGGTGAGTGATGTGAATGGTGATGAGTATGGG-GCTAGCGTCGACCGTACG715'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S1, the Ru-modified oligonucleotide, bound S. pneumoniae R6 with slightly lower affinity than S9.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry125 ± 91 nMDetectionSELEX and Mod-SELEXT=dURu(bpy)TP1, 5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1900 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS9ACACCCCCCAGGATCATAAATTCTCTCGCTGTAGC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S9 bound better to the bacterial target than the modified sequence S1.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry118 ± 1 nMDetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_1901 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS11ACCAGCCATAGTCACATCAAAACTAACTCACATTC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).S11 exhibited a lower propensity at binding to the bacterial target.10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow Cytometry541 ± 1 nMDetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1902 412051602025Selection of Ruthenium Polypyridyl Complex-Modified Aptamers for Photodynamic Therapy against Streptococcus PneumoniaS13CCCATACTCATCACCATTCACATCACTCACCTAGC355'-CGTACGGTCGACGCTAGC-35N-CACGTGGAGCTCGGATGC-3'ssDNAStreptococcus Pneumoniae R6Whole cellAptamer-functionalized ruthenium polypyridyl complexes against S. pneumoniae with targeted photodynamic therapy (PDT).N/A10 rounds (with standard DNA chemistry) and 12 (for the mod-SELEX)Flow CytometryN/ADetectionSELEX and Mod-SELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_1958 205825872010Selection and characterization of DNA aptamers with binding selectivity to Campylobacter jejuni using whole-cell SELEXONS-23N/AN/A5'-GTATACGTATTACCTGCAGC-N40-CGATATCTCGGAGATCTTGC-3'ssDNACampylobacter Jejuni (A9a)Whole cell (bind via proteinaceous nature for cell surface target)Identify aptamers against C. jejuni and detect them in a mixed cell population.ONS-23 showed high binding affinity (25–36%) for all other C. jejuni strains screened (inclusivity) and low apparent binding affinity (1–5%) with non-C. jejuni strains (exclusivity).10Flow Cytometry292.8 ± 53.1 nMDetectionWhole Cell-SELEX5'-FAM LabeledN/AN/AN/AN/AWO Patent WO/2011/097420
ABdb_1964 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK2N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Significantly decreased macrophage invasion from 64.58% (without aptamers) to 33.02% (with 10th aptamer pool), and 26.01% (with NK2).10Flow Cytometry31 ± 4 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_1965 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK1N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry48 ± 13 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1966 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK7N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry35 ± 9 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1967 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK8N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry107 ± 44 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1968 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK10N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry95 ± 28 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_1969 216437492012Aptamer inhibits Mycobacterium tuberculosis (H37Rv) invasion of macrophageNK20N/AN/AN/AssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 93009)Outer membrane protein (OMP)Inhibits the H37Rv invasion of macrophages in vitro and activates the infection of macrophages.Binding affinities are as follows: NK2 > NK7 > NK1 > NK20 > NK10 > NK8.10Flow Cytometry55 ± 16 nMTherapeuticsWhole Cell-SELEXFITC LabeledN/AN/AN/AN/AN/A
ABdb_2017 271512932016Identification of ssDNA aptamers specific to clinical isolates of Streptococcus mutans strains with different cariogenicityH19N/AN/A5'-GCAATGGTACGGTACTTCC-45N-CAAAAGTGCACGCTACTTTGCTAA-3'ssDNAStreptococcus MutansWhole cellIdentify a high-affinity aptamer against cariogenic S. mutans strains.Aptamer H19 showed strong binding, as indicated by higher fluorescence intensity, with Strains 12 and 17 compared with other strains.9Flow Cytometry69.45 ± 38.53 nMDetectionSubtractive SELEX5'-FAM LabeledN/AN/AN/AN/AN/A