Determination of affinity method : Equilibrium Binding Assay

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AptBacDB_ID ⇅	PMID/DOI ⇅	Year ⇅	Title ⇅	Aptamer name ⇅	Sequence (5′ to 3′) ⇅	Length ⇅	Library ⇅	Type ⇅	Target Organism ⇅	Target ⇅	Objective/Mechanism ⇅	Outcome/Inhibitory effect ⇅	No. of selection round SELEX ⇅	Determination of affinity method ⇅	Kd value ⇅	Activity Role ⇅	Method ⇅	Modification ⇅	Cytotoxicity (cell viability) ⇅	Stability⇅	Potential Candidate ⇅	Half-life ⇅	Patent ⇅
ABdb_1971	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	4936–4	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin A (TcdA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	3.1 nM	Detection	SELEX	5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1972	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	4939–280	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin A (TcdA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	1.6 nM	Detection	SELEX	5-naphthylmethylaminocarbonyl-dU (NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1973	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	4943–51	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin A (TcdA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.	8	Equilibrium Binding Assay	1.3 nM	Detection	SELEX	5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1974	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5564–89	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin A (TcdA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	9.8 nM	Detection	SELEX	5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1975	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5570–54	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin A (TcdA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	6.6 nM	Detection	SELEX	5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1976	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	4937–55	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin B (TcdB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	0.76 nM	Detection	SELEX	5-tyrosylaminocarbonyl-dU (TyrdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1977	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	4938–17	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin B (TcdB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	0.45 nM	Detection	SELEX	5-benzylaminocarbonyl-dU (BndU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1978	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	4940–23	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin B (TcdB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.	8	Equilibrium Binding Assay	0.07 nM	Detection	SELEX	5-naphthylmethylaminocarbonyl-dU (NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1979	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	4944–30	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin B (TcdB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	0.08 nM	Detection	SELEX	5-tryptaminocarbonyl-dU (TrpdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1980	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5566–74	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin B (TcdB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	0.04 nM	Detection	SELEX	5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1981	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5573–4	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Toxin B (TcdB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	0.07 nM	Detection	SELEX	5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1982	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	4758–6	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Binary toxin, A chain (CdtA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	0.22 nM	Detection	SELEX	5-tryptaminocarbonyl-dU (TrpdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1983	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5574–49	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Binary toxin, A chain (CdtA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	1.1 nM	Detection	SELEX	5-phenylethyl-1-aminocarbonyl (PEdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1984	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5579–11	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Binary toxin, A chain (CdtA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	0.05 nM	Detection	SELEX	5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1985	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5579–12	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Binary toxin, A chain (CdtA)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	Developed toxin assays show a limit of detection of 1 pmol/L (300 pg/mL) and a 3-log dynamic range.	8	Equilibrium Binding Assay	0.69 nM	Detection	SELEX	5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	Best Candidate	N/A	N/A
ABdb_1986	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5556–51	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Binary toxin, B chain (CdtB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	6.9 nM	Detection	SELEX	5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A
ABdb_1987	23680240	2013	Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers	5556–67	N/A	N/A	N/A	ssDNA	Clostridium Difficile	Binary toxin, B chain (CdtB)	Identified slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin.	N/A	8	Equilibrium Binding Assay	4.4 nM	Detection	SELEX	5-(2-naphthylmethyl)aminocarbonyl (2NapdU) and 5′photocleavable biotin, a D-spacer and Cyanine3 (Cy3) Labeled; 3’-Inverted Thymidine (3'-idT)	N/A	N/A	N/A	N/A	N/A