AptBacterialDB

Determination of affinity method : Enzyme-Linked Aptamer Assay (ELAA)

Total records found: 7

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0080 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 4RATCCGTCACACCTGCTCT-ACGGCGCTCCCAACAGGCCTCTCCTTACGGCATATTA-TGGTGTTGGCTCCCGTAT735'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.Detect as few as 30 live, unlabeled E. coli per ml using FRET assay.5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/ABest CandidateN/AN/A
ABdb_0081 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 3RATCCGTCACACCTGCTCT-GTCTGCGAGCGGGGCGCGGGCCCGGCGGGGGATGCG-TGGTGTTGGCTCCCGTAT725'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.N/A5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/AN/AN/AN/A
ABdb_0082 204430502010A novel screening method for competitive FRET-aptamers applied to E. coli assay developmentEcO 8FATACGGGAGCCAACACCA-CGACTAACACGACCGTTGGGGGGGGCTCGCGCGGGC-AGAGCAGGTGTGACGGAT725'-ATACGGGAGCCAACACCA-N36-AGAGCAGGTGTGACGGAT-3'ssDNAEscherichia Coli (E. Coli) strain 8739 (Crooks strain)Outer membrane protein (OMP)Identify highly effective DNA aptamers for detecting E. coli outer membrane proteins.N/A5Fluorescence Resonance Energy Transfer Assay (FRET) and Enzyme-Linked Aptamer Assay (ELAA)N/ABiosensorMagnetic Bead (MB)-based SELEX5'-Biotinylated and AlexaFluor 647 LabeledN/AN/AN/AN/AN/A
ABdb_0756 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS3ATACCAGCTTATTCAATT-CCCGCCTCTGAGCATTATTAATGTTATACCTTACGGCTGG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.Inhibited PBMCs proliferation with an inhibition rate of 84% and reduced IFN-γ, TNF-α, IL-2, and IL-6 secretions by 89%, 87%, 73%, 69%, respectively. PEGS3 allowed 79% of the mice to survive the lethal challenge.9Enzyme-Linked Aptamer Assay (ELAA)36.93 ± 7.29 nMTherapeutics/ImmunmodulatorySELEX5′-Polyethylene glycol (PEG) with 5'-Amidation (NH₂) and 3’-Inverted Thymidine (3'-idT) and 5'-Biotinylated or 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0757 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS6ATACCAGCTTATTCAATT-GCCGCGGAATTGTGGCTTGGTGTCGTGGTCAGGGGCTGG-AGATAGTAAGTGCAATCT755'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.N/A9Enzyme-Linked Aptamer Assay (ELAA)N/ATherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/ABest CandidateN/AN/A
ABdb_0758 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS12ATACCAGCTTATTCAATT-GCCACTGTGGACTTGTTATTCGTCTTTGCCGTCTAATTCG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.Inhibited PBMCs proliferation with an inhibition rate of 84%.9Enzyme-Linked Aptamer Assay (ELAA)79.35 ± 12.09 nMTherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A
ABdb_0759 271794222016Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonistS23ATACCAGCTTATTCAATT-GCAACAATGTTGTGTCAGCAAGTTGGTTGGCGGTGTATTG-AGATAGTAAGTGCAATCT765'-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3'ssDNAStaphylococcus aureus (S. aureus)Staphylococcal Enterotoxin A (SEA)Identify aptamers that bind to and block superantigen activity of SEA.N/A9Enzyme-Linked Aptamer Assay (ELAA)N/ATherapeutics/ImmunmodulatorySELEX5'-Biotinylated and 5'-FAM LabeledN/AN/AN/AN/AN/A