AptBacterialDB

Determination of affinity method : Bioluminescence-based Membrane Blotting Assay

Total records found: 28

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0289 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA101ATTACTTACGCTATCTAAttt-GGGGGGTGGGTTGTTTGGGATGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0290 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA102ATTACTTACGCTATCTAAttt-GGGGGGGGAACATGTTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting Assay3.5 nM (for B4) and 1.4 nM (for DL1d-A6)DetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0291 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA103ATTACTTACGCTATCTAAttt-GGGGCGGGACTTATTTGGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0292 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA104ATTACTTACGCTATCTAAttt-GGGGGTGGGTGCTTTTGTGGTGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0293 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA105ATTACTTACGCTATCTAAttt-TAGGGGTGGGTTCAATTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0294 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA106ATTACTTACGCTATCTAAttt-GGGGGGCGGGTATTAATGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0295 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA107ATTACTTACGCTATCTAAttt-GTTTTCGTGGGGGGGTGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0296 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA108ATTACTTACGCTATCTAAttt-GCACTAAAGGGGAGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0297 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA109ATTACTTACGCTATCTAAttt-TTGCTTTAGGGGAGGTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting Assay7.7 nM (for B4) and 4.1 nM (for DL1d-A6)DetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0298 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA110ATTACTTACGCTATCTAAttt-TCGCTGATGGGGAGGAGGGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0299 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA111ATTACTTACGCTATCTAAttt-GCCCGATGGGGGTGGCGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0300 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G02ATTACTTACGCTATCTAAttt-TTGCTGTAGGGGAGGAGGGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Displayed the highest specificity, exhibiting a 36% higher specificity index than that of the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/ABest CandidateN/AN/A
ABdb_0301 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G19ATTACTTACGCTATCTAAttt-TTTTTCGGGGGGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0302 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G14ATTACTTACGCTATCTAAttt-TTGCTTTAGAGGAGGCGGGTGGAG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0303 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G11ATTACTTACGCTATCTAAttt-TCGGTGATGGGGAGGAGGCGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0304 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G03ATTACTTACGCTATCTAAttt-GTTTTATGGGGGTGGCGTGTGGCG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0305 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G06ATTACTTACGCTATCTAAttt-TCGCTGATGGGGAGGAGCGTGGGT-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0306 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G10ATTACTTACGCTATCTAAttt-TTACTTTTGGGGGGGCGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0307 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G02ATTACTTACGCTATCTAAttt-GCACGTTTGGGGAGGTTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0308 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G09ATTACTTACGCTATCTAAttt-TTTTTCGAGGGGAGATTGGGGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0309 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G20ATTACTTACGCTATCTAAttt-GCGCGAGTGGGGGGGTGGGTGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0310 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G04ATTACTTACGCTATCTAAttt-GCCCGCGTCGGGGGGTGGGGGGTC-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0311 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA1G01ATTACTTACGCTATCTAAttt-TCGCTATGGGGGTGGCGGCTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.Showed up to 26% higher specificity index compared with the original PmA109.6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0312 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G17ATTACTTACGCTATCTAAttt-TAGCTTTAGGGGTCGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0313 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G08ATTACTTACGCTATCTAAttt-GAGCTTTAGAGGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0314 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G04ATTACTTACGCTATCTAAttt-TTCCTTGGGGAGTGGTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0315 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G06ATTACTTACGCTATCTAAttt-GTTTTCGGGGGCTGGTGGTTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A
ABdb_0316 235687522013In silico maturation of binding-specificity of DNA aptamers against Proteus mirabilisPmA2G09ATTACTTACGCTATCTAAttt-TGGCTCGGGGGGTGTTGGGTGGGG-tttTTATCATCTGGTATGTTA665'-ATTACTTACGCTATCTAAttt-N24-tttTTATCATCTGGTATGTTA-3'ssDNAProteus Mirabilis (B4 and DL1d-A6 cells)Whole cellIdentify aptamers that show high affinity for P. mirabilis and improved affinity using ISM.N/A6Bioluminescence-based Membrane Blotting AssayN/ADetectionWhole Cell-SELEX with In Silico Maturation (ISM)N/AN/AN/AN/AN/AN/A