AptBacterialDB

Determination of affinity method : Bio-Layer Interferometry (BLI)

Total records found: 28

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_0552 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12UGUAAUUCUGCCAUUCUUUUUGGGGCGGAAUACAGGAUGU405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.fmA12-functionalized AgNPs resulted in specific S. aureus antimicrobial activity with only a 1.6 ± 1.0% survival of 12598 cells and 51.9 ± 2.0% survival of 10832 cells.8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)95 nM (by SPR) and 67.1 ± 6.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/A76% fmA12 survives in 10% mouse serum at 37°C over 24 h, and in 50% serum, 85.0% fmA12 survives after 5 h.N/A~52 h for fmA12.N/A
ABdb_0553 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmF07AUACGCUGCUCACUAAAGUCGGGAGUACUCCAAUGUUUUC405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)38.9 ± 4.1 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/ABest CandidateN/AN/A
ABdb_0554 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmE09AUCUAGCAAGCACUUGCAAACAUUCGAUAACGGGGAUUC395'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)418 ± 112 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0555 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmG12UGCCAAUGUAACUCAACACUCUUCAUUGAGCUGUCGGGCA405'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI)475 nM (by SPR) and 220 ± 24 nM (by BLI)Targeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0556 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ3N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.7 ± 3.5 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0557 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ6N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)63.1 ± 7.3 μMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0558 254437902015Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterialsfmA12Δ9N/AN/A5'-TAATACGACTCACTATAGGGAGAGAACAATGACCTG-N40-GAGTGCATTGCATCACGTCAGTAG-3'ssRNAStaphylococcus aureus (S. aureus)Staphylococcus aureus Protein A (SpA)Identify 2′-fully modified aptamers against SpA and deliver aggregation-prone silver nanoparticles (AgNPs) to S. aureus for SpA-dependent cell killing.N/A8Bio-Layer Interferometry (BLI)69.1 ± 16.7 nMTargeted Delivery/TherapeuticsSELEX2'-O-methyl and 2'-Fluoro pyrimidines (2′-F-dGTP and 2′-OMe-dATP/dCTP/dUTP); 5'-Amidation (NH₂)N/AN/AN/AN/AN/A
ABdb_0633 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisMA1GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.Sandwich ELISA based on MA2 and MA1 display a linear range of 8 × 10(4) to 128 × 10(4) CFU with the detection limit of 1 × 10(4) CFU.10Bio-Layer Interferometry (BLI)12.02 nMBiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0634 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisMA2GGGAGCTCAGAATAAACGCTCAA-TATCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.Sandwich ELISA based on MA2 and MA1 display a linear range of 8 × 10(4) to 128 × 10(4) CFU with the detection limit of 1 × 10(4) CFU.10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0635 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE6-15GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACCGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/ABest CandidateN/AN/A
ABdb_0636 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-3GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACCGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0637 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-4GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGACTGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0638 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE7-12GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAGTAGACTGGTGGCTCTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0639 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP9-5GGGAGCTCAGAATAAACGCTCAA-CGGATTAGTCAATAGTCTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0640 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisE9-16GGGAGCTCAGAATAAACGCTCAA-CGGGCTAGTCAATAGACTGGTGGCTTTGTGTGGTG-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0641 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP6-5GGGAGCTCAGAATAAACGCTCAA-TATCCCTATTCCGCTCCATGCTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0642 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP10-4GGGAGCTCAGAATAAACGCTCAA-TACCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_0643 261945582015Identification and application of ssDNA aptamers against H₃₇Rv in the detection of Mycobacterium tuberculosisP10-8GGGAGCTCAGAATAAACGCTCAA-CATCCCTATTCCGCTCCATGTTGCGTACCCGTGCC-TTCGACATGAGGCCCGGATC785'-GGGAGCTCAGAATAAACGCTCAA-N35-TTCGACATGAGGCCCGGATC-3'ssDNAMycobacterium Tuberculosis (H37Rv) (ATCC 27294)Whole cellIdentify aptamers to effectively capture or discriminate MTB strains.N/A10Bio-Layer Interferometry (BLI)N/ABiosensorWhole Cell-SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1188 288878142018Construction and application of an electrochemical biosensor based on an endotoxin aptamerEAQ2ATGAGAGCGTCGGTGTGGTATGCTCACCCCCTGCGGCGCCGTTACGCGGTCCTTGTGTAGGAGGGTGCGGAAGTA75N/AssDNAEscherichia Coli (E. Coli) 055:B5 (L2880)Lipopolysaccharide (LPS)An electrochemical aptasensor was developed using an aptamer assembled on a gold electrode surface using 3-mercaptopropionic acid (MPA) as an intermediate linker to detect endotoxin.N/AN/ABio-Layer Interferometry (BLI)32.5 nMBiosensorN/A5'-Amidation (NH₂) and 5'-BiotinylatedN/AN/AN/AN/AN/A
ABdb_1189 288878142018Construction and application of an electrochemical biosensor based on an endotoxin aptamerEAQ3ATGAGAGCGTCGGTGTGGTATCGCCGCGCTCGCCGATCGTTCCTCCCCCCCCGTCTGTAGGAGGGTGCGGAAGTA75N/AssDNAEscherichia Coli (E. Coli) 055:B5 (L2880)Lipopolysaccharide (LPS)An electrochemical aptasensor was developed using an aptamer assembled on a gold electrode surface using 3-mercaptopropionic acid (MPA) as an intermediate linker to detect endotoxin.Showed a low detection limit of 0.001 EU/mL and a linear range of 0.001–1 EU/mL.N/ABio-Layer Interferometry (BLI)22.9 nMBiosensorN/A5'-Amidation (NH₂) and 5'-BiotinylatedN/AThe biosensor maintained a steady EIS response value after 1 week of storage in the PBS (10 mM, pH 7.4) at 4°C, and the response value still occupied 90%.Best CandidateN/AN/A
ABdb_1344 310331562019Inkjet Printed Nanopatterned Aptamer-Based Sensors for Improved Optical Detection of Foodborne Pathogensa-aptamerATCAAATGTGCAGATATCAAGACGATTTGTACAAGATCCATGCTGAGGTGGTCATAGCTGATCCTACC68N/AssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDeveloped an inkjet-printed nanopatterned aptamer-based sensor for optical detection of E. coli O157:H7.Display a LOD of 25 CFU/mL in pure culture and 233 CFU/mL in ground beef.N/ABio-Layer Interferometry (BLI)N/ABiosensorN/A5'-Carboxy and 5'-Biotin-TEGN/AN/AN/AN/AN/A
ABdb_1404 326859852020Electrochemical aptasensor using boron-carbon nanorods decorated by nickel nanoparticles for detection of E. coli O157:H7Anti-E. coli O157:H7 aptamerATCCAGAGTGACGCAGCA-GGGTGGCGAGACTGGGCGGGTGTCGGGAAGTGAACCGGTGGCGTG-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7Whole cellDeveloped a label-free impedimetric aptasensor for the detection of E. coli O157:H7 employing boron-carbon nanorods decorated by nickel nanoparticles (BC-Ni) nanostructured platform.Detect E. coli O157:H7 selectively with a detection limit of 10 cfu and a dynamic detection range of 10(0) to 10(5) cfu in water, juice, and faecal samples.15Bio-Layer Interferometry (BLI)69.73 nMBiosensorMicrotiter Plate-based Cell SELEX5'-Biotinylated and 5'-FITC LabeledN/AN/AN/AN/AN/A
ABdb_1470 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx1.1)ATCCAGAGTGACGCAGCA-GTAGTTTGTTGGTTATTACGGCGGGTTGCGATGGGTGCGAATCGG-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx1 (Stx1.1)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.LOD of 44.5 pg/mL for stx1 with minimal cross-reactivity and a dynamic response range from 50 pg/mL to 100 ng/mL.7Bio-Layer Interferometry (BLI)8.27 nM (of peptide) and 47.2 pM (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_1471 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx1.3)ATCCAGAGTGACGCAGCA-GGATAGGACGTCAAATTAGGGCCCGGTACAACGAAAGCCCACAAC-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx1 (Stx1.2)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.N/A7Bio-Layer Interferometry (BLI)31.0 nM (of peptide) and 30.3 μΜ (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1472 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx2.1)ATCCAGAGTGACGCAGCA-GGGGGCAGGTTCATGGCTTGGGTGCGGTGGGCATGATTCGTGGTG-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx2 (Stx2.1)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.N/A7Bio-Layer Interferometry (BLI)45.2 nM (of peptide) and 83.8 nM (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1473 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx2.2)ATCCAGAGTGACGCAGCA-TGTATCTCTTACTTAAGCCTTTGGTTCGGTAACAGCCTGGCATGC-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx2 (Stx2.2)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.N/A7Bio-Layer Interferometry (BLI)110 nM (of peptide) and 25.2 μM (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/AN/AN/AN/A
ABdb_1474 328142082020Biolayer interferometry-SELEX for Shiga toxin antigenic-peptide aptamers & detection via chitosan-WSe2 aptasensorApt(Stx2.3)ATCCAGAGTGACGCAGCA-GGAAAGGACGTCAAATTAGGGGCCGGGACAACGAAAGCCCACAAC-TGGACACGGTGGCTTAGT815'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O157:H7 str. SakaiShiga toxin subtype stx2 (Stx2.3)Identify anti-Shiga toxin aptamers and develop an Apta/Chito-WSe2 voltammetric detection platform.LOD of 41.3 pg/mL for stx2 with minimal cross-reactivity and a dynamic response range from 50 pg/mL to 100 ng/mL.7Bio-Layer Interferometry (BLI)4.6 nM (of peptide) and 28.6 pM (of protein)DetectionBiolayer Interferometry based-SELEX (BLI-SELEX)N/AN/AN/ABest CandidateN/AN/A
ABdb_2112 287280092017Bridged Rebar Graphene functionalized aptasensor for pathogenic E. coli O78:K80:H11 detectionAnti-E.coli aptamerN/AN/A5'-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3'ssDNAEscherichia Coli (E. Coli) O78:K80:H11 (MTCC 726)Whole cellA novel fabrication method of functionalised Bridged Rebar Graphene (BRG) onto EIS-based nanostructured aptasensor for label-free detection of E. coli O78:K80:H11.LOD ~ 10(1) cfu/mL with a dynamic response range from 10(1) to 10(6) cfu/mL.12Bio-Layer Interferometry (BLI)14 nMBiosensorPhenylboronic acid (PBA) mediated SELEX5'-FITC LabeledN/AN/AN/AN/AN/A