AptBacterialDB

Determination of affinity method : Bead-based Affinity Chromatography

Total records found: 8

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AptBacDB_ID ⇅ PMID/DOI ⇅ Year ⇅ Title ⇅ Aptamer name ⇅ Sequence (5′ to 3′) ⇅ Length ⇅ Library ⇅ Type ⇅ Target Organism ⇅ Target ⇅ Objective/Mechanism ⇅ Outcome/Inhibitory effect ⇅ No. of selection round SELEX ⇅ Determination of affinity method ⇅ Kd value ⇅ Activity Role ⇅ Method ⇅ Modification ⇅ Cytotoxicity (cell viability) ⇅ Stability⇅ Potential Candidate ⇅ Half-life ⇅ Patent ⇅
ABdb_1040 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT916CATCCGTCACACCTGCTC-GGCAAAAAGGATTGCCCAGGTCTGCTGTCTAGCCGGATTC-GGTGTTGGCTCCCGTATC765'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.Detection limits of 2.1 ng/ml in buffer and 2.4 ng/ml in tap water, with a linear range from about 1 ng/ml to 1000 ng/ml.12Bead-based Affinity Chromatography48.5 ± 0.5  nMBiosensorSELEXAmidation (NH₂-(CH2)6) or Biotinylated and AlexaFluor 488 LabeledN/AN/ABest CandidateN/AN/A
ABdb_1041 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT910CATCCGTCACACCTGCTC-GTACACAGGAGCCAAACCCAACCAGACCAACCTGAAGCC-GGTGTTGGCTCCCGTATC755'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography54.6 ± 6.7 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1042 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT928CATCCGTCACACCTGCTC-CACCCGTCACACTTATCCGTCAACTGGGCTCCCACTGGG-GGTGTTGGCTCCCGTATC755'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography23.6 ± 1.2 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1043 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT931CATCCGTCACACCTGCTC-CCAGCGTGCGTCGAGGGGGACCCCTGTCAGCCCCCTCGCG-GGTGTTGGCTCCCGTATC765'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography50.2 ± 13.2 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1044 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT938CATCCGTCACACCTGCTC-CACCCGTCCACTCATCCGTCACACCTGTTCTCCCCATG-GGTGTTGGCTCCCGTATC745'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography23.2 ± 0.4 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1045 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT939CATCCGTCACACCTGCTC-CACCCGTCACACTTATCCGTCACACTGGGCCCCACTGGG-GGTGTTGGCTCCCGTATC755'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography41.6 ± 4.0 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1046 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT943CATCCGTCACACCTGCTC-CACCCGTCCACTCATCCGACACACCTGCTCTCCCCACTG-GGTGTTGGCTCCCGTATC755'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography26.8 ± 0.5 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A
ABdb_1047 294082002018Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAACT946CATCCGTCACACCTGCTC-CCCACAACCGGTCCGGTGTTCGGTCCCCGTATCACGCCCC-GGTGTTGGCTCCCGTATC765'-CATCCGTCACACCTGCTC-N40-GGTGTTGGCTCCCGTATC-3'ssDNAVibrio CholeraeCholera toxin (AB5-type toxin composed of a donut-shaped homopentameric B subunit (CT-B))Identify aptamers against and develop an MB-based sandwich ELAA to detect CT-B from spiked tap water.N/A12Bead-based Affinity Chromatography30.4 ± 1.5 nMBiosensorSELEXAlexaFluor 488 LabeledN/AN/AN/AN/AN/A