Primary information |
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ID | antitb_1001 |
Peptide Name | Polydim-I |
Sequence | AVAGEKLWLLPHLLKMLLTPTP |
N-terminal Modification | Free |
C-terminal Modification | Free |
Chemical Modification | None |
Linear/ Cyclic | Linear |
Length | 22 |
Chirality | L |
Nature | Amphipathic |
Source | Natural |
Origin | Isolated from the venom of the Neotropical wasp Polybia dimorpha |
Species | Mycobacterium abscessus subsp. massiliense |
Strain | Mycobacterium abscessus subsp. massiliense isolates GO 01, GO 06, GO 07, GO 08, GO 13, and GO 18, CRM0020 (Mycobacterium abscessus subsp. massiliense), and ATCC19977 (Mycobacterium abscessus subsp. abscessus) |
Inhibition Concentartion | MIC = 60.8 μg/mL |
In vitro/In vivo | Both |
Cell Line | Peritoneal macrophages, J774 macrophages cells, human red blood cells |
Inhibition Concentartion | Treatment of infected macrophages with 7.6 μg/mL of Polydim-I decreased approximately 50% of the bacterial load |
Cytotoxicity | Non-cytotoxic, 10% cytotoxicity on J774 cells at concentrations above 121.6 μg/mL (10%) and concentrations up to 121.6 μg/mL presented less than 2.5% of hemolytic activity |
In vivo Model | 6 to 8 weeks old BALB/c and IFN-γKO (Knockout) female mice |
Lethal Dose | 2 mg/kg/mLW shows 90% reduction in bacterial load |
Immune Response | NA |
Mechanism of Action | Cell wall disruption |
Target | Cell wall |
Combination Therapy | None |
Other Activities | NA |
Pubmed ID | 26930596 |
Year of Publication | 2016 |
3-D Structure | View in Jmol or Download Structure |