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Secondary Structure Annotation
This module allow users to predict regular secondary structure in their protein. Basically secondary structures are assigned in a query protein based on its similarity with known protein structure in PDB. This module allow users to select desired BLAST parameters for performing similarity search. Advanced option further facilitate users in selecting most appropriate parameters. There are three non-redundant database againt which BLAST can be performed: 40%, 70% and 100%. The secondary structure information is displayed according to DSSP defination. User can select whether to perform annotation based on eight DSSP states or standard three states. In order to obtain more information on secondary structure states of DSSP
Click here
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Sequence Submission Form
Paste your Protein sequence in FASTA format (single sequence only)
Select number of top BLAST hits for annotation:
10
20
30
40
Expect Value (e-value):
1e-100
1e-10
1e-5
0.01
0.1
1
10
100
500
1000
2000
Weight Matrix:
BLOSUM80
BLOSUM62
BLOSUM45
PAM30
PAM70
Select level of redundancy
40%
70%
100%
Select secondary structure states
Eight DSSP state
[H,G,I,B,E,T,S,C]
Standard 3-states
[H=(G,H,I); E=(B,E); C=(T,S)]