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Details of TopicalPdb ID 1424

PRIMARY INFORMATION
ID1424
PMID2127341
Year1990
SequenceFA
NameThiol based metalloproteinase inhibitor
Length2
N-Terminal ModificationHSCH2 (DL)CH[CH2CH(CH3)2]CO
C-Terminal ModificationAmidation
Linear/ CyclicLinear
ChiralityD and L both were used separately
Chemical ModificationHSCH2 (DL)CH[CH2CH(CH3)2]CO-Phe-Ala-NH2 is the complete sequence with modifications
Origin of PeptideSynthetic thiol based metalloproteinase inhibitor
Nature of Peptide/CargoPotent inhibitor of corneal coliagenase, alkali-induced corneal ulceration and P. aeruginosa elastase
MechanismSynthetic thiol based metalloproteinase inhibitor
Cargo Sequence/StructureNone
Name of cargo
Not applicable
AssayPeptidase activity checked via enzyme assay, Clinical evaluation and scoring, Statistical analysis
Enhancerinhibitors were dissolved in 95% ethanol containing 1 mM acetic acid immediately prior to use,
Properties of enhancerNot mentioned
Concentration1mM solution of peptide dissolved in 95% ethanol containing 1mM acetic acid immediately prior to use
Incubation time26 hours
Tissue permeability (value with units)Average clinical score according to hours-post inoculation for topically and subconjunctivally applied bacterial inoculum(treatment till 26 hours post inoculation): Inhibitor=5.2/Control=5.8, where, 0=No corneal pathology; 1=Comeal infiltrate without significant melting; 2=Grade I melting (none to one-third of the corneal surface); 3=Grade II melting (one-third to two-thirds of the corneal surface) and/or mild bulging;
Tissue SampleOne eye of the New Zealand White rabbits weighing between 2 and 2.5 kg was used as test and the other as control
Ex vivo/In vivo/In vitroin vitro
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN.A.