| PRIMARY INFORMATION |
|---|
| ID | 1424 |
| PMID | 2127341 |
| Year | 1990 |
| Sequence | FA |
| Name | Thiol based metalloproteinase inhibitor |
| Length | 2 |
| N-Terminal Modification | HSCH2 (DL)CH[CH2CH(CH3)2]CO |
| C-Terminal Modification | Amidation |
| Linear/ Cyclic | Linear |
| Chirality | D and L both were used separately |
| Chemical Modification | HSCH2 (DL)CH[CH2CH(CH3)2]CO-Phe-Ala-NH2 is the complete sequence with modifications |
| Origin of Peptide | Synthetic thiol based metalloproteinase inhibitor |
| Nature of Peptide/Cargo | Potent inhibitor of corneal coliagenase, alkali-induced corneal ulceration and P. aeruginosa elastase |
| Mechanism | Synthetic thiol based metalloproteinase inhibitor |
| Cargo Sequence/Structure | None |
Name of cargo
| Not applicable |
| Assay | Peptidase activity checked via enzyme assay, Clinical evaluation and scoring, Statistical analysis |
| Enhancer | inhibitors were dissolved in 95% ethanol containing 1 mM acetic acid immediately prior to use, |
| Properties of enhancer | Not mentioned |
| Concentration | 1mM solution of peptide dissolved in 95% ethanol containing 1mM acetic acid immediately prior to use |
| Incubation time | 26 hours |
| Tissue permeability (value with units) | Average clinical score according to hours-post inoculation for topically and subconjunctivally applied bacterial inoculum(treatment till 26 hours post inoculation): Inhibitor=5.2/Control=5.8, where, 0=No corneal pathology; 1=Comeal infiltrate without significant melting; 2=Grade I melting (none to one-third of the corneal surface); 3=Grade II melting (one-third to two-thirds of the corneal surface) and/or mild bulging; |
| Tissue Sample | One eye of the New Zealand White rabbits weighing between 2 and 2.5 kg was used as test and the other as control |
| Ex vivo/In vivo/In vitro | in vitro |
| SECONDARY INFORMATION |
|---|
| STRUCTURE | |
| SMILES | N.A. |
